plants of Sinningia sp were used as starting material (fig.2). For disinfection of foliar material was used Domestos 10% for. 30min, then rinsed five times in sterile.
Annals of RSCB
Vol. XIV, Issue 2
IN VITRO MULTIPLICATION AND ACCLIMATIZATION AT SINNINGIA HYBRIDA Lia Mladin, Oana Sicora, C. Chis, M. Caprar, G. Hranean, C. Sicora BIOLOGICAL RESEARCH CENTER, BOTANICAL GARDEN “VASILE FATI”, JIBOU, ROMANIA
Summary At the Biological Research Center Jibou we multiplied in vitro Sinningia hibrida in order to support the microproduction of ornamental plants from our Botanical Garden. We succeeded to obtain mature plants in a relatively short time, using two special MS media, for culture initiation and for rooting. The survival rate using this method was around 80%. Key words: Sinningia, in vitro multiplication, acclimatization, ornamental plant microproduction
Introduction Theoretical discoveries in plant tissue and cell culture field are in constant relationship with the practical applicability of the results. At the Biological Research Center from Jibou, in vitro plant material supports the microproduction of ornamental plants. Sinningia is a valued plant for the beauty and color of its flowers and it was included in the replication plan for the year 2009. Sinningia speciosa (Gloxinia), from Gesneriaceae Familiy, was brought to Europe at the beginning of the XIX-th centrury from Brazil and is the specia from which come most existing varieties in culture (1). It is a plant with short flower stem, hairy oval leaves, toothed on the edges. Abundant flowers are large, soft, bell shaped and delicate fringed on the edges. Their color may vary from white to pink and purple. The most spectacular gloxinias are bicolor: light color outside and a more intense color inside. So, there are cultivars with dark purple flower with white neck, dark purple embroidered with white, mostly red embroidered with white (fig.1).
Fig.1. Sinningia’s cultivars
Sinningia hybrida summarizes most cultivars. Taking into account the amount of decorative flowers and their size, the following varieties were chosen to be multiplied: Emperor William dark purple embroidered with, Emperor Frederick, red flower embroidered with white, Crispa meteor with red flower.
Materials and methods The leaves of medium size young plants of Sinningia sp were used as starting material (fig.2). For disinfection of foliar material was used Domestos 10% for 30min, then rinsed five times in sterile water (3). 289
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Media used were MS supplemented with 0,5ml/l ANA, 2ml/l BAP, sucrose 30g/l, agar4g/l, pH 5,8, for initial step and MS supplemented with 2ml/l ANA, sucrose 30g/l, agar 4g/l, pH 5,8 for rooting.
Results and disscution Culture was started using a lot of mother plants grown in one of the modules of pilot greenhouses. In vitro cultures are initiated from the foliar fragments, and in a relatively short time (8-12weeks) are produced plants ready to be repassed on rooting media (2). Thus, within 3-4 months is possible to obtain plants for acclimatization, able to bloom in the same year.
Fig. 3. Foliar fragments
In 10 weeks the cuttings developed a large number of plantulae, with a typical conformation, that filled the culture flask (fig.4).
Fig. 2. Mother plant Fig. 4. Large number of plantulae
The difference between classical culture of seed tubers and cuttings of leaves is that micropropagation in vitro provides in about the same time (8-9 months) plant material about 30 times larger on a smaller area. Per 1 square meter, about 100 foliar fragments can be initiated (using 50ml Erlenmeyer flask) that can generate in approximate 20 weeks up to 3000 finished plants. The disinfected leaves were cut into 1-2cm2 (fig. 3) pieces and placed on MS medium.
These plantulae were transferred on MS media for rooting (fig.5).
Fig. 5. Shoot on MS rooting media
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Annals of RSCB
Vol. XIV, Issue 2
For acclimatization step (fig.6 and 7) was used a substrate composed of peat and perlite (50:50), a photoperiod of 16h, temperature between 220C-250C and relative humidity of 80-90%. In the first two weeks the pots with seedlings were kept in the incubator in order to keep the humidity high, a very important factor in acclimatization (4).
Conclusions 1. The MS media supplemented with 0,5ml/l ANA, 2ml/l BAP was effective for micropropagation of selected Sinningia cultivars. 2. The MS media chosen for rooting, MS supplemented with 2ml/l ANA has provided a large percentage of rooted seedlings 3. In a short time (approximate 6 months) were obtained mature plants, with an 80% survival rate.
Reference Elena Selaru, Plante de apartament, Ed. Ceres, Bucuresti, 2004. Dorina Cachita-Cosma, Corneliu Petrescu, Curs practice de culture de tesuturi in vitro cu aplicatii in legumicultura si floricultura, Curs postuniversitar, 1985; Elena Rakosy-Tican, Inginerie genetica vegetala, Ed. Casa Cartii de Stiinta, Cluj-Napoca, 2005; Dorina Cachita-Cosma, Constantin Deliu, Lenuta Rakosy-Tican, Aurel Ardelean, Tratat de biotehnologie vegetala, Ed. Dacia, Cluj Napoca, 2004;
Fig. 6. Initial step of acclimatization
Fig. 7. Final step of acclimatization
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