Profile of the cellular immune response induced in

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CBA (Cytometric Bead Array) in spleen cells from previously immunized ... were collected by the BD FACSDIVA® software and analyzed using the. FlowJo X® ...
Profile of the cellular immune response induced in C57Bl/6j mice immunized with the association of different strains of Toxoplasma gondii tachyzoites irradiated by Cobalt-60 Costa,

1 A. ,

Melo,

1 T.S. ;

do Nascimento,

2 N.

& Galisteo Jr,

1,3 A.J.

1. Instituto de Medicina Tropical de São Paulo - IMTSP / USP – Lab. de Protozoologia 2. Instituto de Pesquisas Energéticas e Nucleares – IPEN / Centro de Biotecnologia 3. Hospital das Clínicas da FMUSP – HC / LIM49 e-mail: [email protected] ABSTRACT Toxoplasmosis is a disease described worldwide that affects about 1/5 of the human population. In immunocompetent patients, significant ocular involvement and frequent congenital involvement can occur, with severe sequelae. The disease is acquired by oral ingestion of infectious forms such as cysts and oocysts and the mucous membranes are the first place to combat the agent. In toxoplasmosis, the use of irradiated parasites induces effective post-challenge protective immunity using T. gondii cystogenic strains. In this work we evaluated the cellular immune response of C57Bl/6j mice immunized simultaneously with three different strains of T. gondii tachyzoites irradiated at 255 Gy of Cobalt 60 (Co-60). We quantified the production of cytokines: IL-10, IL-6, IL-4, TNF-alpha, IFN-γ and IL-2 by CBA (Cytometric Bead Array) in spleen cells from previously immunized mice. The cytokine profile showed a classical profile of Th1 response with a typical increase of IFN-γ in relation to the other cytokines in all groups evaluated. In the groups immunized with the VEG 255 Gy strain and the RH+VEG 255 Gy and RH+ME-49+VEG 255 Gy groups we observed a significant difference in IL-2 production (p