e-proceeding - VIT

MESSAGE FROM PRESIDENT BioMET-2018 International Conference is being organized, by the Center for Biomaterials, Cellular and Molecular Theranostics (CBCMT), Vellore Institute of Technology (VIT), Vellore, India on thematic topics of “Bio-Materials, Bio-Engineering and Bio-Theranostics” from 24th -28th, July, 2018. Two days pre-conference workshops are organized on 24th and 25th July followed by three days conference from 26th July –28th July. It gives me great pleasure to welcome both International and National delegates to Vellore Institute of Technology, Vellore, India. The Society for Biomaterials and Artificial Organs (SBAOI), was established three decades ago, to bring the biomaterial, medical device professionals and clinicians to a common platform and to promote health care related research in India. The conference is held annually and this year BioMET 2018 is being held at VIT, Vellore. BioMET 2018 provides a platform where large number of researchers across the globe will gather to deliver plenary, invited talks and poster presentations on various thematic topics. I am sure that professionals and budding young researchers will be benefited from the deliberations and would contribute to various emerging areas of research. We have 41 speakers from 14 countries and 40 speakers from India, apart from clinicians and industry. A special Research Industry Networking (RIN) session is arranged to debate on challenges and identify collaborators to work on solutions. Special session on paper writing is being conducted by M/S Springer. Other important events being organized are an interactive session with young scientists and a talk on IPR. I congratulate the organizing committee members for their tireless efforts in organizing BioMET 2018. I am sure the conference will be a great success. Finally, a warm welcome to all the delegates. Enjoy your stay in Vellore.

(Veena Koul)

MESSAGE Dear Colleagues,

It is with the greatest pleasure that we welcome you to Vellore, Tamil Nadu, India, and to the 1st BIOMET Congress. This is YOUR meeting and on behalf of the Organizing Committee and the many committed scientists, industry partners, and other associates who have contributed to building this event, it is our wish that you enjoy and live this experience as if it was your first international conference. The unique organizing team at VIT, the committed International College of Faculty Members, and the different Committees, have poured their heart and soul into dreaming, planning and finalizing this meeting with us for the past 2 years.

The furnished and up-to-date Tutorials, the list of international and national speakers, the attention to networking, the building of lunch and learns sessions, and many more events were conceived based on the vision of what many of you had conveyed to us as we were planning the meeting. So now you are here, and we encourage you to make the most of it.

This 1st BIOMET Congress is dedicated to the many scientists, engineers and clinicians, from India and worldwide, who have dedicated energies and efforts to building a scientific knowledge that is having impact on people’s health around the world. Biomaterials, bioengineering and biotheranostics saved the life or improved its quality for millions around the globe in the last decades. But many more has yet to be saved, future has never been so promising in this field. Finally, we wish you all a very prosperous and inspiring Congress and hope that we have made your time in Vellore a most worthy and personal experience for you and your colleagues.

International Conference on Biomaterials, BioEngineering & BioTheranostics (BioMET 2018) 24th - 28th July 2018

Pre-Conference Workshop 24th July 2018 Sessions 7:00 AM - 9:00 AM Registration at Ambedkar Auditorium, Technology Tower, Ground Floor 9:00 AM – 9:40 AM Workshop Inauguration at Ambedkar Auditorium, Technology Tower, Ground Floor Session – B1

Session – A1

Venue: Ambedkar Auditorium (Technology Tower, Ground Floor) Stem cells for health 9:45 AM - 1:00 PM Dr. Roberto Fanganiello, Brazil Dr. Ketul.C.Popat, USA Dr. P. V. G. K. Sarma, India.

Session A1 continues

Venue: Kamaraj Auditorium (Technology Tower, Seventh Floor) Biological scaffolds and natural biomaterials for regenerative medicine I 9:45 AM - 1:00 PM Dr. Jayachandran Kizhakkedathu, Canada Dr. Rodrigo S Vieira, Brazil Dr. Nicholas Dunne, Ireland 11:00 AM – 11:15 AM Tea Break Session B1 continues

Session – C1 Venue: Smart Classroom (Technology Tower, Room No 513: Fifth Floor) Surfaces and Interfaces-I: Basics to Advanced Modification/ Characterization 9:45 AM - 1:00 PM Dr. Arun K Kota, USA Dr. Jean J Pireaux, Belgium Dr. Franck Clement, France

Session C1 continues

1:00 PM – 1:50 PM Lunch at Foodys Session – A2 Gene Therapy: From Basics to Challenges 2:00 PM – 4:00 PM Dr. Gabriele Candiani, Italy Dr. Dwaipayan Sen, India

Session A2 continues

Session – B2

Session – E1

Biological scaffolds and Natural Biomaterials for Regenerative Medicine II 2:00 PM – 4:00 PM Dr. Alina Sionkowska, Poland Dr. Francesca Boccafoschi, Italy 3:00 PM – 3:15 PM Tea Break

Degradable Metals for Bio-absorbable Implants

Session B2 continues

Session E1 continues

2:00 PM – 4:00 PM Dr. Maurizio Vedani, Italy Dr. Diego Mantovani, Canada

4:30 PM – 6:30 PM Springer Workshop on “Publishing with Impact” at Ambedkar Auditorium, Technology Tower, Ground Floor 7:00 PM – 8:00 PM Dinner at Greeno

CENTRE FOR BIOMATERIALS, CELLULAR AND MOLECULAR THERANOSTICS VELLORE INSTITUTE OF TECHNOLOGY, VELLORE, INDIA.


Session – B3

Pre-Conference Workshop 25th July 2018 Sessions Session – D1

Venue: Ambedkar Auditorium (Technology Tower, Ground Floor)

Venue: Kamaraj Auditorium (Technology Tower, Seventh Floor)

Biofabrication for 3D Bioprinting for Regenerative Medicine 9:00 AM – 12:30 PM Dr. Lakshmi S Nair, USA Dr. Harishkumar Madhyastha, Japan Dr. Abhay Pandit, Ireland Dr. Amit Bandyopadhyay, USA

Antibacterial Approaches in Biomaterials and Health 9:00 AM – 12:30 PM Dr. Gabriele Candiani, Italy Dr. Thomas J Webster, USA Dr. Diego Mantovani, Canada

Session – E2 Venue: Smart Classroom (Technology Tower, Room No 513: Fifth Floor) Advanced Biomaterials for Next Generation of Implants 9:00 AM – 12:30 PM Dr. AP Rosifini, Brazil Dr. Ramesh Raghavendra, Ireland Dr. Ketul C Popat, USA

11:00 AM – 11:15 AM Tea Break Session B3 continues

Session D1 continues


12:30 PM – 1:20 PM Lunch at Foodys Session – C2 Surfaces and Interfaces-II: Surface Modifications Interfacing Biology 1:30 PM – 5:00 PM Dr. Michael Tatoulian, France Dr. Yasuharu Ohgoe, Japan Dr. Jose Ricardo Ferreira, Brazil

Session – E3

Session – D2

Advanced Biomaterials for Next Generation of Implants II

Translation Research, Commercialization & Regulatory

1:30 PM – 5:00 PM Dr. Thomas J Webster Dr. Dheepa Srinivasan, India Dr. L A Rocha , Brazil Dr. Ana Ribeiro, Brazil

1:30 PM – 5:00 PM Dr. Raul Rosales Ibanez, Mexico Dr. Shirley Motaung, South Africa Dr. Francesca Boccafoschi, Italy

3:00 PM – 3:15 PM Tea Break Session C2 continues


Session D2 continues

7:00 PM – 8:00 PM Dinner at Greeno



DAY 1: 26th July 2018 Morning Session 7:00 AM - 9:00 AM Registration at Technology Tower, Ground Floor 9:00 AM – 9:15 AM Conference Introduction Session – I Venue: Ambedkar Auditorium (Technology Tower, Ground Floor) Theme: Advances in Biomaterials

Session - II Venue: Kamaraj Auditorium (Technology Tower, Seventh Floor) Theme: Surface Engineering I Surfaces and Interfaces

Session – III Venue: VOC Gallery (Technology Tower, Second Floor) Theme: 3D Printing /Tissue Engineering

Chair: Dr. Caroline Richard 9:30 AM – 9:55 AM Invited talk on "Redefining Identity of Disease, Tissues and Cells – A Biomaterials Paradigm" by Dr. Abhay Pandit, National University of Ireland, Galway, Ireland. 9:55 AM – 10:20 AM Invited talk on "Design, Fabricating and Commercializing in-the-body Nano sensors: The Future of Health" by Dr. Thomas J Webster, Northeastern University, Boston, USA.

Chair: Alina Sionkowska 9:30 AM – 9:55 AM Invited talk on "Superomniphobic Surfaces: Design & Applications" by Dr. Arun Kota, Colorado State University, USA.

Chair: Dr. M. Nageswara Rao 9:30 AM -9:55 AM Invited talk on “Clinical Significance of 3D Printing in Bone Health” by Dr. Susmita Bose, Washington State University, USA.

9:55 AM – 10:20 AM Invited talk on "Plasma Processing for Surface Modification: Biomedical Applications" by Dr. Michael Tatoulian, PSL Research University, Paris, France.

9:55 AM – 10:20 AM Invited talk on "Thinking beyond ‘Black-Box’: Quantitative Process Physics of 3D Inkjet Powder Printing and Adaptability to Bioceramic Scaffolds Unveiled" by Dr. Bikramjit Basu, Indian Institute of Science, Bangalore, India. 10:20 AM – 10:45 AM Invited talk on "Current Trends and Ireland's Activities on 3D Printing of Biomaterials" by Dr. Ramesh Raghavendra, South Eastern Applied Materials Research Centre, Ireland.

10:20 AM – 10:45 AM Invited talk on "Advanced Metallic Alloys and Modern Processing Techniques in the Biodegradable Material Field" by Dr. Maurizio Vedani, Politecnico di Milano, Italy.

10:20 AM – 10:45 AM Invited talk on "Micro/NanoEngineering of Material Surfaces for Tissue Engineering and Regenerative Medicine" by Dr. Ketul C Popat, Colorado State University, USA.



Session – I Venue: Ambedkar Auditorium (Technology Tower, Ground Floor) Theme: Advances in Biomaterials Chair: Dr. Caroline Richard 10:45 AM – 11:10 AM Invited talk on "Engineering of Biopolymers for Smart Healthcare" by Dr. Bhuvanesh Gupta, Bioengineering Laboratory, Department of Textile Technology, Indian Institute of Technology, New Delhi, India.

DAY 1: 26th July 2018 Morning Session Session - II Session – III Venue: Kamaraj Auditorium Venue: VOC Gallery (Technology Tower, Seventh Floor) (Technology Tower, Second Floor) Theme: Surface Engineering I Theme: 3D Printing /Tissue Engineering Surfaces and Interfaces Chair: Dr. Alina Sionkowska 10:45 AM – 11:10 AM Invited talk on "Effect of Plasma Treatment and Foreign Element Contents on A -C:H Film Depositions for Biomedical Applications" by Dr. Yasuharu Ohgoe, Tokyo Denki University, Japan.

Chair: Dr. M. Nageswara Rao 10:45 AM – 11:10 AM Invited talk on "Fabrication of Nanostructured Biomaterial Implants: The Art of the Possible" by Dr. Bala Vaidhyanathan, Loughborough University, U.K.

11:10 AM – 11: 30 AM Tea Break 11:30 AM – 11:55 AM

11:30 AM – 11:55 AM Invited talk on "Targeting DDR Kinases: Novel Therapeutics Towards Cancer Treatment" by Dr. Sivapriya Kirubakaran, IIT Gandhinagar, Gujarat, India. 11:55 AM – 12:20 PM Invited talk on "3D Triple Cell Culture in Bioreactors from Collagen Gel Scaffolds: Innovation in Advanced In Vitro Models, Cosmetics and Regenerative Medicine" by Dr. Diego Mantovani, Laval University, Quebec City, Canada.

Invited talk on "Cold Atmospheric Plasmas for Biomedical Applications. Afterglows and Ionization Waves in Biology and Medicine" By Dr. Franck Clement, Pau University- Uppa, France. 11:55 AM – 12:20 PM Invited talk on "Engineering MultiBiofunctional Materials for Orthopaedic Applications" By Dr. Kaushik Chatterjee, Indian Institute of Science, Bangalore, India.

11:30 AM – 11:55 AM Invited talk on "Repair of Alveolar Cleft Defect in Vietnamese Pigs with Mesenchymal Stem Cells and Biohybrid Scaffolds: A Preliminary Report" By Dr. Raul Rosalez Ibanez, Tissue Engineering Lab, Mexico City, Mexico. 11:55 AM – 12:20 PM Invited talk on "Smart Nanocuetical Hybrid in Tissue Resilience during Diabetic Wound Healing" by Dr. Harish Kumar Madhyastha, Miyazaki University, Miyazaki, Japan.

12:30 PM - 1:00 PM Lunch at CS Hall 1:00 PM - 2:00 PM Poster and Sponsor Exhibition at Anna Auditorium



DAY 1: 26th July 2018 Afternoon Session Session - IV Session - V Session – VI Venue: Ambedkar Auditorium Venue: Kamaraj Auditorium Venue: VOC Gallery (Technology Tower, Ground Floor) (Technology Tower, Seventh Floor) (Technology Tower, Second Floor) Theme: Mechanobiology and Theme: Clinical Translation Theme: Advances in Nanotechnology Biomechanics Chair: Dr. Louise M. Jennings

Chair: Dr. Diego Mantovani

Chair: Dr. Kaushik Chatterjee

2:20 PM – 2:45 PM Invited talk on "’Fault lines and foot faults’, Ankle and foot biomechanics in Bipedalism” by Dr. Henry Prakash M, Christian Medical College, Vellore, India. . 2:45 PM – 3:10 PM

2:20 PM – 2:45 PM Invited talk on “Bench to bed translational research in BiomaterialsAre we in the right direction?” by Dr. Geetha Manivasagam, VIT, Vellore.

2:20 PM – 2:45 PM Invited talk on "Biopolymeric Composites for Biomedical and Cosmetic Applications" by Dr. Alina Sionkowska, Nicolaus Copernicus University in Torun, Poland.

2:45 PM – 3:10 PM

2:45 PM – 3:10 PM

Invited talk on "Neuromuscular Control, Injury and Rehabilitation" by Dr. Suresh Devasahayam, Christian Medical College, Vellore, India.

Invited talk on “Biomaterials in Orthopedics” by Dr. Rex Chandra Bose, Rex Ortho Hospital, Coimbatore, India.

3:10 PM – 3:35 PM Invited talk on “Rehabilitation Engineering in Clinical Practices” by Dr. Rajdeep Ojha, Department of Bioengineering Christian Medical College, Vellore, India.

3:10 PM – 3:35 PM Invited talk on "The Holy Grail Of Articular Cartilage Regeneration Current Status And Challenges" by Dr.Manasseh Nithyananth, Department of Orthopedics, Christian Medical College, Vellore, India.

3:35 PM – 4:00 PM Invited talk on "Design And Development of Passive Polycentric Knee Joint and Ankle Joint for Transfemoral Amputees" by Dr. Kanagaraj S, IIT Guwahati, India.

3:35 PM – 4:00 PM Invited talk on “Clinical Translation of Maxillofacial Anomaly” by Dr. Manikandhan R, Meenakshi Ammal Dental College and Hospital, Chennai, India.

Invited talk on "The Two Faces of Titanium Dioxide Nanoparticles BioCamouflage in Human Osteoblastic Cell Models" by Dr. Ana Lopes Ribeiro, University of Grande Rio, Brazil. 3:10 PM – 3:35 PM Invited talk on "Surface Modifications of New Titanium Alloys for Biomedical Applications - Bulk and Surface Properties" By Dr. Ana Paula Rosifini Alves Claro, Universidade Estadual Paulista (Unesp), Guaratingueta Campus, Brazil. 3:35 PM – 4:00 PM Invited talk on "Multifunctional CaP Nanocarriers for Management of Bone Infections" by Dr. Sampath Kumar TS, IIT Madras, India.

4:00 PM - 4:20 PM Tea Break at Anna Auditorium



DAY 1: 26th July 2018 4:30 PM – 5:30 PM Inaugural Ceremony at Anna Auditorium 5:30 PM – 5:45 PM MoU signing at Anna Auditorium 5:45 PM – 6:00 PM Group photo at Anna Auditorium 6:00 PM – 6:45 PM Plenary Lecture Chair: Dr. C P Sharma "TRANSLATING MOLECULAR BIOENGINEERING FROM THE LAB TO THE PATIENT" by Dr. Ashutosh Chilkoti, Duke University, USA C P Sharma Awardee (SBAOI) for 2018 Venue: Anna Auditorium 7:00 PM - 8:00 PM Conference Dinner at Foodys



Session - VII Venue: Ambedkar Auditorium (Technology Tower, Ground Floor)

DAY 2: 27th July 2018 Morning Session Session – VIII Session – IX Venue: Kamaraj Auditorium Venue: VOC Gallery (Technology Tower, Seventh Floor) (Technology Tower, Second Floor) Theme: Commercialization of Medical Devices

Theme: Drug Delivery

Theme: Cell and Gene Therapy

Chair: Dr. Abhay Pandit 9:00 AM - 9:25 AM Invited Talk on "Redox Sensitive Nano Platforms for Drug and Gene Delivery" by Dr. Veena Koul, IIT Delhi, India.

Chair: Dr. Jose Ricardo Muniz Ferreira 9:00 AM - 9:25 AM Invited Talk on "Non-Viral Gene Delivery Vectors: What is known and what is not" by Dr. Gabriele Candiani, Politecnico Di Milano, Milan, Italy.

Chair: Dr. Bikramjit Basu 9:00 AM - 9:25 AM Invited Talk on "Studies on Deformation of Pre-Contoured Fracture Fixation Plates for their Applications in Orthopaedic Surgeries" by Dr. Prasad Yarlagadda, Queensland University of Technology, Australia.

9:25 AM – 9:50 AM Invited talk on "Injectable Hydrogel Systems with Tunable Properties for Biomedical Applications" by Dr. Lakshmi S Nair, University of Connecticut, USA.

9:25 AM – 9:50 AM Invited Talk on "Fluorescent Biosensors for Cellular Dynamics and Discoveries in Natural Light Sensing and Eye Brain Regeneration" by Dr. Akash Gulyani, Institute of stem cell biology and regenerative medicine, Bangalore, India. 9:50 AM – 10:15 AM Invited Talk on "Therapeutic genome editing for beta hemoglobinopathies” by Dr. Mohankumar K Murugesan, CSCR, CMC, Vellore.

9:25 AM – 9:50 AM Invited Talk on "Development of Health Care Products: Nutraceuticals and Radioprotectors" by Dr. Indira Priyadarsini, Bhabha Atomic Research Centre, Mumbai, India.

9:50 AM – 10:15 AM Invited Talk on “Carbon Dots for Drug Delivery and Cellular Microenvironment Sensing" by Dr. Neetu Singh, IIT Delhi, India. 10:15 AM – 10: 40 AM Invited talk on “NanoparticleMediated Peptide-Conjugated Targeted Drug Delivery in Breast Cancer” by Dr. Gopal C Kundu, National Centre For Cell Science, Pune, India.

9:50 AM – 10:15 AM Invited Talk on "An Insight in to Magnesium Based Materials for Biomedical Applications" by Dr. Manoj Gupta, National university of Singapore, Singapore.

10:15 AM – 10: 40 AM Invited talk on “Breaking the Barriers with Cell and Gene Therapy: From Research to Reward” by Dr. Dwaipayan Sen, VIT, Vellore, India.

10:40 AM - 10:55 AM Tea Break


International Conference on Biomaterials, BioEngineering & BioTheranostics (BioMET 2018) Session - X Venue: Ambedkar Auditorium (Technology Tower, Ground Floor)

24th - 28th July 2018 DAY 2: 27th July 2018 Morning Session Session – XI Session – XII Venue: Kamaraj Auditorium Venue: VOC Gallery (Technology Tower, Seventh Floor) (Technology Tower, Second Floor)

Theme: Tissue Engineering -II

Theme: Regenerative medicine I

Theme: BioTribology and BioCorrosion

Chair: Dr. Veena Koul 10:55 AM - 11:20 AM Invited Talk on "Injectable Biomimitic Whitlockite Nanoparticles in bone" by Dr. R. Jayakumar, Amrita Vishwa Vidyapeetham, Kerala, India.

Chair: Dr. Yasuharu Ohgoe 10:55 AM - 11:20 AM Invited Talk on "Active Coatings For Cardiovascular Stent Applications: from Materials and Surface Science to Biological Performances" by Dr. Jean Jacques Pireaux, University of Namur, Belgium.

11: 20 AM – 11:45 AM Invited Talk on "IVD Degeneration and its Consequences-an Injectable Tissue-Engineered Therapy" by Dr. Annie John, University of Kerala, India.

11: 20 AM – 11:45 AM Invited Talk on "Transcriptional and Molecular Events Underlying Heterogeneity in the Osteopotential of Human Mesenchymal Stromal Cells" by Dr. Roberto Dalto Fanganiello, University of Sao Paulo, Brazil. 11:45 AM – 12:10 PM

Chair: Dr. Caroline Richard 10:55 AM - 11:20 AM Invited Talk on "Engineering '50 active years after 50' through MultiDisciplinary Research, Innovation, Knowledge Creation and Translation" by Dr. Louise M. Jennings, Institute of Medical and Biological Engineering, University of Leeds, Leeds, UK. 11: 20 AM – 11:45 AM Invited Talk on "Pre-clinical Assessment of an All Polymer Knee Replacement" by Dr. Raelene Cowie, Institute of Medical and Biological Engineering, University of Leeds, Leeds, UK.

11:45 AM – 12:10 PM Invited Talk on "Three Dimensional Porous, Biomolecule Supplemented Silk-PVA Composite Scaffold Reinforced with Natural Eggshell Membrane for Human Knee Meniscal Tissue Engineering" by Dr. R. Selvakumar, PSG Institute of Advanced Studies, Coimbatore. 12:10 PM – 12: 35 PM Invited Talk on "Bio-Additive Manufacturing" by Dr. Arumaikkannu, Anna University, Tamilnadu, India. 12:35 PM – 1: 00 PM Invited talk on "Application for Additive Manufacturing Towards Improving Human Comfort” by Dr. Rajesh Ranganathan, Coimbatore Institute of Technology, Coimbatore.

11:45 AM – 12:10 PM

Invited Talk on "Sulfonated-Modified Chitosan for Blood-Interacting Material" by Rodrigo S. Vieira University of Ceara, Brazil.

Invited Talk on "Tribocorrosion of Doped Titanium dioxide nanotubes thin films Developed for Osseointegrated Implants" by Dr. Luís Augusto Rocha, UNESP - Universidade Estadual Paulista, Sao Paulo, Brazil.

12:10 PM – 12: 35 PM Invited Talk on “Optical Coherence Tomography Use in Optimisation of Coronary Angioplasty” by Dr. Binoy John, MD DM, Credence Hospital, Trivandrum, Kerala, India. 12:35 PM – 1: 00 PM Invited Talk on "Long Acting Polymer Therapeutics for Systemic Iron Excretion" by Dr. Jayachandran Kizhakkedathu, The University of British Columbia, Vancouver, BC, Canada. 1:00 PM - 1:30 PM Lunch at CS Hall

12:10 PM – 12: 35 PM Invited talk on “Quantum MechanicsApplications to Devices, Materials and Mechanisms” by Dr. R. Asokamani, Tamil Nadu Academy of Sciences, Chennai, India.


International Conference on Biomaterials, BioEngineering & BioTheranostics (BioMET 2018) 24th - 28th July 2018 DAY 2: 27th July 2018 Afternoon Session 1:30 PM - 2:30PM Poster Session and Exhibition at Anna Auditorium / CS Hall (150 Posters and Stalls by Companies) Session - XIII Session – XIV Session – XV Venue: Ambedkar Auditorium (Technology Tower, Ground Floor) Theme: Stem cell technology/applications

Venue: Kamaraj Auditorium (Technology Tower, Seventh Floor)

Venue: VOC Gallery (Technology Tower, Second Floor)

Theme: Bioceramics

Theme: Drug Delivery – II

Chair: Dr. Gopal C Kundu

Chair: Dr. Bala Vaidhyanathan

Chair: Dr. Jean Jacques Pireaux

2:40 PM - 3:05 PM Invited Talk on "How Dental Pulp Stem Cells can Open New Avenues in the Development of New Tissue Engineering Strategies?" by Dr. Jose Ricardo Muniz Ferreira, Sao Paulo, Brazil. 3:05 PM – 3:30 PM Invited Talk on "Biological dysfunctions in vascular aneurysms: who is the guilty?" by Dr. Francesca Boccafoschi, University of Piemonte Orientale, Novara, Italy. 3:30 PM – 3:55 PM

2:40 PM - 3:05 PM Invited Talk on "Biological and Luminescence Response of TelluriteLanthanide-Reinforced Hydroxyapatite Composites" by Dr. Sooraj Hussain Nandyala, University of Birmingham, UK.

2:40 PM - 3:05 PM Invited talk on "Engineering 3D Approaches to Model Cancer Metastasis" by Dr. S C Kundu, University of Minho, Portugal.

3:05 PM – 3:30 PM Invited Talk on "Nanostructured Bio active glass: From Synthesis to Materials design for Biomedical Applications" by Dr. Balakumar S, National centre for Nanoscience and nano technology, Chennai, India. 3:30 PM – 3:55 PM

3:05 PM – 3:30 PM Invited Talk on "Electrospun nanotextiles as Drug Eluting Implantable Depot for Ovarian Cancer therapy" by Dr. Deepthy Menon, Amrita Vishwa Vidyapeetham, Kerala, India. 3:30 PM – 3:55 PM

Invited Talk on "Bioceramics From Orthopedic To Oncology” by Dr. A.M. Ballamurugan, Bharathiar University, Coimbatore, India.

Invited Talk on "The Role of Plant Doped Scaffolds for Tissue Engineering" by Dr. Michael Pillay, Vaal University of Technology, Vanderbijilpark, South Africa.

Invited Talk on "Human CD34+ Cells Differentiate to Multiple Cell Lineages Making it an Effective Cell in the Regenerative medicine" by Dr. P. V. G. K. Sarma, SVIMS, Tirupati, India.

4:00PM - 4:20PM Tea Break 4:25 PM – 6:00 PM 4:25 PM – 6:00 PM 4:25 PM – 6:00 PM Rapid Fire Session Rapid Fire Session Rapid Fire Session Oral presentations by participants Oral presentations by participants Oral presentations by participants 6:30 PM to 7:15 PM Cultural Program at Anna Auditorium Performance by MYTHRI-Centre for Arts, Chennai & CBCMT Dance Team 7:30 PM to 8:30 PM Conference Dinner at Foodys



DAY 2: 27th July 2018 Afternoon Session 3:00 PM – 5:00 PM General Body Meeting of Society for Biomaterials and Artificial Organs and Society for Tissue Engineering and Regenerative Medicine Venue: Technology Tower, Ground Floor, Shakespeare Gallery

DAY 2: 27th July 2018 Afternoon Session Young Scientist Meeting 5.15 PM - 6.15 PM Technology Tower, Room No 311: Third Floor Dr. Bikramjit Basu, IISc, Bangalore Dr. Debrupa Lahiri Dr. Sonia Kapoor

Dr. Rohidas Arote Dr. Saralasrita Mohanty

Dr. Sanjay Singh Dr. Ashutosh Dubey Dr. Saurabh Gupta Dr. Niladri Maiti

Dr. Falguni Pati Dr. Amit Jaiswal Dr. Amisha Desai Dr. Durgalakshmi D

CBCMT Centre Members 6:30 PM to 7:15 PM Cultural Program at Anna Auditorium Performance by MYTHRI-Centre for Arts, Chennai & CBCMT Dance Team 7:00 PM to 8:00 PM Conference Dinner at Foodys



DAY 3: 28th July 2018 Morning Session Session – XVI Session - XVII Venue: Ambedkar Auditorium (Technology Tower, Ground Floor)

Venue: Smart Classroom (Technology Tower Room No.: 513, Fifth Floor)

Theme: Tissue Engineering –III

Theme: Regenerative Medicine -II

Chair: Dr. Jayachandran Kizhakkedathu

Chair: Dr. Francesca Boccafoschi

9:00 AM – 9:25 AM Invited talk on "Advanced Biomaterials for Tissue Engineering and Regenerative Medicine: Innovations, Interventions and Applications" by Dr. Ashok Kumar, IIT Kanpur, India.

9:00 AM – 9:25 AM Invited Talk on "Scaffold Fabrication for Bone Tissue Regeneration" by Dr. A. M. Kuthe, Visvesvaraya National Institute of Technology, Nagpur, India.

9:25 AM – 9:50 AM Invited Talk on "Electrospun Nanofibrous Polymer, Bioceramic, Graphene Scaffolds" by Dr. T. M. Sridhar, University of Madras, Chennai, India.

9:25 AM – 9:50 AM Invited Talk on "The Potential of Medicinal Plants in the Development of Tissue Engineering Products" by Dr. Shirley Motaung, Tshwane University of Technology, Pretoria, South Africa.

9:50 AM - 10:00 AM Tea Break


International Conference on Biomaterials, BioEngineering & BioTheranostics (BioMET 2018) 24th - 28th July 2018 DAY 3: 28th July 2018 Morning Session Young Scientist Forum Venue: Kamaraj Auditorium, (Technology Tower, Seventh Floor) Chair: Dr. R Asokamani 9:00 AM – 9:15 AM Invited talk on “Surface Engineering of Metallic Orthopaedic Implants for Sustained Drug Release” by Dr. Debrupa Lahiri, IIT Roorkee, India. 9:15 AM – 9:30 AM Invited talk on “Targeting Alternatively Activated m2 Macrophages Using Protein Nanoparticles: an Approach to Modulate Tumor Microenvironment” by Dr. Sonia Kapoor, Amity Institute of Technology, Noida, India. 9:30 AM – 9:45 AM Invited talk on “Gold-core/ cerium-oxide Shell Nanostructure: A Redox Active Nanozyme Mimicking Triple Enzyme Activities”, by Dr. Sanjay Singh, Institute of Life Sciences, Ahmedabad University, India. 9:45 AM – 10:00 AM Invited talk on “Piezoelectric Bioimplants for Polarized Bone Applications” by Dr. Ashutosh Kumar Dubey, IIT (BHU), Varanasi, India. 10:00 AM – 10:15 AM Invited talk on “Zirconia- The Implant Material For Future” by Dr. Saurabh Gupta, IAOCI, Bangalore, India. 10:15 AM – 10:30 AM Invited talk on “A Study on the Effect of Acid Catalyst in the Synthesis of Bioglass 45S5 Composition Prepared by Sol-Gel Method” by Dr. Durgalakshmi D, Anna University, India. 10:30 AM - 10:45 AM Tea Break Young Scientist Forum Venue: Kamaraj Auditorium, (Technology Tower, Seventh Floor) Chair: Dr. Ketul C Popat 10:45 AM – 11:00 AM Invited talk on “Design of Multifunctional Nanomaterials for Cancer Gene Therapy” by Dr. Rohidas Arote, Seol National University, Republic of Korea. 11:00 AM – 11:15 AM Invited talk on “BioMechanical Analysis of the CNC Machined Alumina Based Monolith Dental Crown” by Dr. Saralasrita Mohanty, NISER, Bhubaneswar, India. 11:15 AM – 11:30 AM Invited talk on “3D Bioprinted In Vitro Tissue/Organ Models” by Dr. Falguni Pati, IIT Hyderabad, India. 11:30 AM – 11:45 AM Invited talk on “Plasmonic Nanorattles: Engineering its Structure for Applications in Catalysis, Sensing and Theranostics” by Dr. Amit Jaiswal, IIT Mandi, India. 11:45 AM – 12:00 PM Invited talk on “The Functional Performance of Biological (Decellularised) Heart Valve Roots” by Amisha Desai, University of Leeds, Leeds, UK.


International Conference on Biomaterials, BioEngineering & BioTheranostics (BioMET 2018) 24th - 28th July 2018 DAY 3: 28th July 2018 Morning Session 10:10 AM - 12:40 AM Research Industry Network Venue: Ambedkar Auditorium (Technology Tower, Ground Floor) 10:10 AM – 10:20 AM Inauguration of RIN session by Dr Anita Aggarwal. Sci E, TDT, DST Introduction to RIN by co-organizer Dr. Kaladhar K, Amrita School of Pharmacy, Kerala, India. Presentation of Bezwada Biomedical, USA 10:20 AM – 10:30 AM Talk by representative from Hi Media 10:30 AM – 10:40 AM Talk by Mr. Aroop Kumar Dutta, Director, ExCel Matrix Biological Devices P. Ltd 10:40 AM – 10:50 AM Talk by representative from Carl Zeiss 10:50 AM – 11: 00 AM Talk by representative from Jayon 11:00 AM – 11:10 AM Talk by representative from Anton Paar 11:10 AM – 11:20 AM Talk by Dr. Debdutt Patro, Ducom Instruments 11:20 AM – 11:30 AM Talk by Dr. Sachin J. Shenoy, SCTIMST, Kerala, India. 11:30 AM – 11:40 AM Talk by Dr. Sabitha M, Amrita School of Pharmacy, Kerala, India. 11:40 AM – 12:40 PM Panel Discussion on Clinical challenges in health care / medical device Venue: Ambedkar Auditorium (Technology Tower, Ground Floor) Members: Jayon Implants, TTK Healthcare, CTARS, Mr. Aroop Kumar Dutta (ExCel matrix), Dr. Debdutt Patro (Ducom), Dr. Pradeep Poonnoose (CMC Vellore), Dr. Suresh Devasahayam (CMC), Dr. Subha Narayan Rath (IIT Hyderabad), Dr. Deepak Shivarathre (Sparsh Hospitals, Bangalore) & all Delegates and Participants 12:50 PM – 1:20 PM Lunch at CS Hall DAY 3: 28th July 2018 Afternoon Session Chair: Dr. Geetha Manivasagam 1:30PM – 2:00 PM Invited Talk on "Introduction to Center for Biomaterials at Korea Institute of Science and Technology" by Dr. Seok Hyun-kwang , Korea Institute of Science and Technology, South Korea. Venue: Anna Auditorium 2:00 PM – 2:20 PM Invited Talk by Kalyanaraman Subramaniam, Director, Intellectual Property Mentors Group, Bangalore, India. Venue: Anna Auditorium 2:20 PM – 2:40 PM Invited Talk by Dr. D. Viswanadham, Chief Manager - Innovation, IKP Knowledge Park, Hyderabad, India. Venue: Anna Auditorium Valedictory Function (2:45PM - 3:30PM) Venue: Anna Auditorium CENTRE FOR BIOMATERIALS, CELLULAR AND MOLECULAR THERANOSTICS VELLORE INSTITUTE OF TECHNOLOGY, VELLORE, INDIA.

Contents S.No Speaker Name

Topic of presentation

Page No

Session- I 1

Dr. Abhay Pandit

2

Dr. Amit Bandyopadhyay

3

Dr. Maurizio Vedani

4

Dr. Nicholas J Dunne

5 6 7

Dr. Bhuvanesh Gupta Dr. Sivapriya Kirubakaran Dr. Diego Mantovani

8

Dr. Arun Kota

9

Dr. Michael Tatoulian

10

Dr. Ketul C Popat

11

Dr. Yasuharu Ohgoe

12

Dr. Franck Clement

13

Dr. Kaushik Chatterjee

14 15

Dr. Susmita Bose Dr. Bikramjit Basu

16

Dr. Ramesh Raghavendra

17

Dr. Bala Vaidhyanathan

18

Dr. Raul Rosalez Ibanez

19

Dr. Harish Kumar Madhyastha

20

Dr. Henry Prakash M

21

Dr. Kanagaraj S

Redefining Identity of Disease, Tissues and Cells – A Biomaterials Paradigm 3D Printing of Hard Materials for Structural and Biomedical Applications Advanced Metallic Alloys and Modern Processing Techniques in the Biodegradable Material Field Calcium Phosphate based Biomaterials for Bone Tissue Engineering Applications Engineering of Biopolymers for Smart Healthcare Targeting DDR Kinases: Novel Therapeutics Towards Cancer Treatment 3D triple cell culture in bioreactors from collagen gel scaffolds: innovation in advanced in vitro models, cosmetics and regenerative medicine Session- II Superomniphobic Surfaces: Design & Applications Plasma Processing for Surface Modification: Biomedical Applications" Micro/Nano-Engineering of Material Surfaces for Tissue Engineering and Regenerative Medicine Effect of Plasma Treatment and Foreign Element Contents on A -C:H Film Depositions for Biomedical Applications Cold Atmospheric Plasmas for Biomedical Applications. Afterglows and Ionization Waves in Biology and Medicine Engineering Multi-Biofunctional Materials for Orthopaedic Applications Session – III Clinical Significance of 3D Printing in Bone Health Thinking beyond ‘Black-Box’: Quantitative Process Physics of 3D Inkjet Powder Printing and Adaptability to Bioceramic Scaffolds Unveiled Current Trends and Ireland's Activities on 3D Printing of Biomaterials Fabrication of Nanostructured Biomaterial Implants: The Art of the Possible Repair of Alveolar Cleft Defect in Vietnamese Pigs with Mesenchymal Stem Cells and Biohybrid Scaffolds: A Preliminary Report Smart Nanocuetical Hybrid in Tissue Resilience during Diabetic Wound Healing Session - IV Fault lines and foot faults’, Ankle and foot biomechanics in Bipedalism Design And Development of Passive Polycentric Knee Joint and Ankle Joint for Transfemoral Amputees"

Session – V

i

1 2 3 3 4 5 6

7 8 9 10

12

13

13 14

15 16 17

18

19 19

22

Dr. Geetha Manivasagam

23

Dr. Manasseh Nithyananth

24

Dr. Alina Sionkowska

25

Dr. Ana Paula Rosifini Alves Claro

26

Dr. Sampath Kumar TS

Dr. Ashutosh Chilkoti 27

28

Dr. Gopal C Kundu

29

Dr. Veena Koul

30

Dr. Lakshmi S Nair

31

Dr. Neetu Singh

32

Dr. Gabriele Candiani

33

Dr. Akash Gulyani

34

Dr. Vijayalakshmi Venkatesan

35

Dr. Thomas J Webster

36

Dr. Indira Priyadarsini

37

Dr. Prasad Yarlagadda

38

Dr. Manoj Gupta

39

Dr. S C Kundu

40

Dr. Annie John

41

Dr. R. Selvakumar

"Bench to bed translational research in biomaterials-Are we in the right direction?" The holy grail of articular cartilage regenerationcurrent status and challenges Session - VI Biopolymeric composites for biomedical and cosmetic applications Surface modifications of new Titanium alloys for biomedical applications - Bulk and Surface properties Multifunctional CaP nanocarriers for management of bone infections Plenary Lecture Translating molecular bioengineering from the lab to the patient Session - VII Nanoparticle-Mediated Peptide-Conjugated Targeted Drug Delivery in Breast Cancer Redox Sensitive Nano Platforms for Drug and Gene Delivery Injectable Hydrogel Systems with Tunable Properties for Biomedical Applications Carbon Dots for Drug Delivery and Cellular Microenvironment Sensing Session – VIII Non-Viral Gene Delivery Vectors: What is known and what is not Fluorescent Biosensors for Cellular Dynamics and Discoveries in Natural Light Sensing and Eye Brain Regeneration Mesenchymal Stem Cells to Avert Preclinical Diabetic state - Our Experience(s) from Obese Mutant Rat Model System Session - IX Design, Fabricating and Commercialzing in-thebody Nano sensors: The Future of Health Development of Health Care Products: Nutraceuticals and Radioprotectors. Studies on Deformation of Pre-Contoured Fracture Fixation Plates for their Applications in Orthopaedic Surgeries An Insight in to Magnesium Based Materials for Biomedical Applications

20 21

21 22

23

24

24 25 26 27

28 29

30

31 32 33

34

Session - X Engineering 3D Approaches to Model Cancer 35 Metastasis" IVD Degeneration and its Consequences-an 36 Injectable Tissue-Engineered Therapy Three Dimensional Porous, Biomolecule 37 Supplemented Silk-PVA Composite Scaffold Reinforced with Natural Eggshell Membrane for

ii

42

Dr. Arumaikkannu

43

Dr. Jean Jacques Pireaux

44

Dr. Roberto Dalto Fanganiello

45

Rodrigo S. Vieira

46

Dr. Jayachandran Kizhakkedathu

47

Dr. Louise M. Jennings

48

Dr. Raelene Cowie

49

Dr. Luis Augsto Rocha

50

Dr. Jose Ricardo Muniz Ferreira

51

Dr. Francesca Boccafoschi

52 Dr. P. V. G. K. Sarma

Human Knee Meniscal Tissue Engineering Bio-Additive Manufacturing Session – XI Active Coatings For Cardiovascular Stent Applications : from Materials and Surface Science to Biological Performances Transcriptional and Molecular Events Underlying Heterogeneity in the Osteopotential of Human Mesenchymal Stromal Cells Sulfonated-Modified Chitosan for BloodInteracting Material Long Acting Polymer Therapeutics for Systemic Iron Excretion Session – XII Engineering '50 active years after 50' through Multi-Disciplinary Research, Innovation, Knowledge Creation and Translation Pre-clinical Assessment of an All Polymer Knee Replacemen" by Dr. Raelene Cowie Tribocorrosion of Doped Titanium dioxide nanotubes thin films Developed for Osseointegrated Implants

38

39

40

41 42

43

44 45

Session - XIII How dental pulp stem cells can open new avenues 46 in the development of new tissue engineering strategies? Decellurized tissues: a promising approach for 47 tissue engineering and regenerative medicine Human CD34+ cells differentiate to multiple cell 48 lineages making it an effective cell in the renegerative medicine

54

Dr. Sooraj Hussian Nandyala Dr. Balakumar S

55

Dr. A. M. Ballamurugan

Session – XIV Biological and Luminescence response of Tellurite- 49 Lanthanide-reinforced hydroxyapatite composite Nanostructured bioactive glass: from synthesis to 50 materials design for biomedical applications Bioceramics from orthopedic to oncology 51

56 57

Dr. R Jayakumar

Injectable biomimitic whitlockite nano particles in bone

53

Session – XV

58

Dr. Deepthy Menon Dr. Michael Pillay

59 60

Dr. Ashok Kumar

61 62

Dr. A. M. Kuthe

Dr. T. M. Sridhar

52 Electrospun nanotextiles as drug eluting 53 implantable depot for ovarian cancer therapy The role of plant doped scaffolds for tissue 54 engineering Session – XVI Advanced biomaterials for tissue engineering and regenerative medicine: Innovations, Interventions Electrospun Nanofibrous Polymer, Bioceramic, and Applications Graphene Scaffolds

56 57

SESSION XVII

Dr. Shirley Motaung

Scaffold fabrication for bone tissue regeneration The potential of medicinal plants in the development of tissue engineering products

iii

58 59

Young scientist forum S.No

Speaker Name

Topic of presentation

Page no

1

Dr. Debrupa Lahiri

60

2

Dr. Sonia Kapoor

3

Dr. Sanjay Singh

4

Dr. Ashutosh Kumar Dubey Dr. Saurabh Gupta

Surface engineering of metallic orthopaedic implants for sustained drug release Targeting alternatively activated M2 macrophages using protein nanoparticles: An approach to modulate tumor microenvironment Gold-Core/Cerium-Oxide shell nanostructure: A redox active nanozyme mimicking triple enzyme activities Piezoelectric bioimplants for polarized bone applications Zirconia - The implant material for future Design of multifunctional nanomaterials for cancer gene therapy Biomechanical analysis of the CNC machined alumina based monolith dental crown 3D Bioprinted in vitro Tissue/Organ models Plasmonic Nanorattles: Engineering its Structure for Applications in Catalysis, Sensing and Theranostics The Functional Performance of Biological (Decellularised) Heart Valve Roots A study on the effect of acid catalyst in the synthesis of bioglass 45S5 composition prepared by sol-gel method

63

5 6 7 8 9 10

Dr. Rohidas Arote Dr. Sarlasrita Mohanty Dr. Falguni Pati Dr. Amit Jaiswal Ms. Amisha Desai

11 Dr. Durga Lakshmi D

iv

61

61

62 63

64 64 65 66 67

RAPID FIRE ORAL PRESENTATIONS S.No

Easy chair abstract no

1

72

Gopinath Perumal, Amrita Anti-bacterial activity of Friction stir processed 68 Chakrabarti, Harpreet Singh austenitic stainless steel with S.coccus and Grewal, Harpreet Singh E.coli Arora, Soumya Pati and Shailja Singh

2

45

Anjana J, Ullas Mony, K.T Shalumon, Bioengineered Braided Multiscale J.P. Chen and R Jayakumar Scaffolds for Tendon Reconstruction

3

173

Arun Kumar Teotia, Aman Nikhil and Critical size cranial defect healing using 69 Ashok Kumar functionalized bilayered scaffold

4

177

Piyali Das, Samit Kumar Nandi, Piyali Development and characterization of 70 Basak, Manoj Kumar R, Debrupa decellularized caprine choncal (auricular) Lahiri and Biswnath Kundu cartilage and its feasibility of nano-mechanical characterization

5

185

Anshu Dubey, Satish Jaiswal and Fabrication, Mechanical and Corrosion 71 Debrupa Lahiri Properties of Functionally Graded Mg-3Zn/HA composite through Spark Plasma Sintering for Biomedical Application

6

198

Gururaj Parande, Vyasaraj Manakari, The effect of microalloyed dysprosium on the 72 Wan Ying Sng and Manoj Gupta mechanical, damping and corrosion behaviour (International) OF Magnesium-Zinc biomedical alloy

7

200

Gururaj Parande, Vyasaraj Manakari Mechanical and corrosion and Manoj Gupta magnesium-zinc-based nanocomposites targeting applications

8

202

Pushpalatha C, Swaroop Hegde and In vitro remineralization effect of novel cocoa Deveswaran R varnish on early enamel carious lesion

9

242

Emon Barua, Ashish B. Deoghare, Effect of pre-treatment and calcination process 74 Payel Deb, Sumit Das Lala and on micro-structural and physico-chemical Sushovan Chatterjee properties of hydroxyapatite derived from chicken bone bio-waste

10

142

Priyadarshani Choudhary and Sujoy K Graphene-based Antimicrobial Coating for 75 Das Biomedical Application

11

115

Rohit Goyal, S. Krishna Prasad and P. Fast Responsive Soft Bio-mimetic Robotic 75 Lakshmi Madhuri Actuators

12

Authors

Title

Page No

Fibrous 68

behaviour of 72 hydroxyapatite orthopaedic

73

Swati Sharma, Nitu Bhaskar, Biomimetic porous high density 76 Suryasarathi Bose, Bikramjit Basu polyethylene/polyethylene-grafted-maleic anhydride scaffold with improved in vitro cytocompatibility

v

13

127

Archini Paruthi, Sanjay and Superb K. Misra

Singh Study on variation in reactivity and bandgap of 77 copper oxide and nickel oxide nanoparticles towards understanding cellular toxicity

14

151

Bupesh Raja V.K., Arja Sri Sai, Bandi In vitro biocompatibility studies on lanthanum Vedaraj Goud and Dr.Manoj Gupta oxide nanoparticles 78

15

152

Aarti R. Deshmukh and Beom Soo Ultrasound assisted green synthesis of silver 78 Kim (international) nanoparticles using fenugreek seeds extract and their antibacterial activity

16

74

Viswanathan Haribabu, Palani Label-free Magneto-fluorescent Nanoclusters 79 Sharmiladevi, Abubacker Sulaiman for Multimodal Imaging Farook, Koyeli Girigoswami and Agnishwar Girigoswami

17

34

R Shiji, M J Manu, K Raveendran Biocompatible and pH sensitive polysaccharide 80 Pillai and T T Sreelekha - gold nanoclusters for real-time tumor imaging

18

94

Radhika Poojari

19

144

Rasmi Ranjan Behera, Apurba Das, Effect of Laser Surface Texturing on 82 Varun Saxena, Pamu Dobbidi, Lalit Bioactivity and Protein Adsorption of Biphasic Pandey and Mamilla Ravi Sankar Calcium Phosphate Coated Ti-6Al-4V Alloy

20

132

Delma Dcruz Kamalasanan

21

133

Jeevna Rajeev, Sabitha Mangalathillam Development of a and Kaladhar Kamalasanan niosomal formulation

22

21

Bappa Maiti

Reduction Responsive Nanovesicles for 84 Efficacious Drug Delivery to Sensitive and Drug Resistant Cancer Cells

23

227

Pasupuleti Santhosh Kumar, Chodimella Chandrasekhar, Lokanathan Srikanth and P.V.G.K. Sarma

In vitro differentiation potential of 84 megakaryocytes to functional platelets from cultured human hematopoietic CD34+ stem cells

24

---

Asish Kumar Panda, Ravi Kumar K., Electrical stimuli mediated stem cell 85 Amanuel Gebrekrstos, Suryasarathi differentiation on PVDF/MWCNT Bose, Bikramjit Basu composite for neural patch application

25

---

Subhadip Basu, Aritri Ghosh, Ananya (Fe/Sr) co-doped biphasic calcium phosphate 86 Barui and Bikramjit Basu with tailored osteoblast cell functionality

26

---

Dhulika Ravinuthala1,2 and Alan M Assessment of Laminin-α2 derived peptides on 86 Punnoose1* cell survival, cell adhesion and differentiation potential of cultured mouse myoblasts cell line.

27

109

Prasoon Kumar, Arpana Panicker and A simple, scalable method of fabrication of 87 Kaushik Chatterjee nanofibrous cell culture inserts by direct deposition of electrospun nanofibers on 3D printed substrates

Smart Nanoengineered Green Materials for Cancer Immunotherapy

and

81

Kaladhar Development of a medicated Kajal based drug 82 delivery system for the prophylaxis of allergic conjunctivitis

vi

novel

aspirin loaded 83

28

123

Yogesh Verma, Ranjan Verma, Ajay Thermo sensitive Poloxamer 407 hydrogel 88 Singh and Gangenahalli Gurudutta containing LiCl, alginate and growth factors, improves hemostasis, wound healing and hair regrowth: Application at high altitudes

29

125

Asheesh Gupta, Gaurav Keshri

30

229

Roopesh Pai, Shiny Velayudhan and Spheroid Sandwich Culture as an In Vitro 89 Anil Kumar P.R Model System for Evaluation of Drug Induced Hepatotoxicity

31

49

Sivashanmugam A, Seunghun S. Lee, Injectable CaSO4-Lactoferrin-Substance P 90 Inseon Kim, Nathaniel S. Hwang and Hydrogel for Enhancing Stem Cell Migration in Jayakumar Rangasamy Cranial Bone Regeneration

32

81

Juhi Chakraborty Ghosh

33

143

Sriya Yeleswarapu, Soumya Sethi, Structural and functional mimicking of 91 Shibu Chameettachal and Falguni Pati intrathoracic airway tissues by 3D printing technology – a roadmap for lung tissue engineering

34

56

Uday Roopavath, Amit Shukla, Surya A Novel One Step Fabrication of Multi- 92 S. and Subha Rath Functional Microfluidic Devices using FDM and DLP based 3D Printing

35

66

Amit Shukla, Eshwaramoorthi, Surya Narayan Rath

36

---

37

---

Srimanta Barui, Asish K. Panda, Kuppuraj R., Saptarshi Basu, Bikramjit Basu Vidushi Sharma, Srimanta Barui, Bikramjit Basu

Prasun Roy and Bioactive Composite Dressings Accelerate 88 Cutaneous Wound Healing

and Dr.Sourabh Chemically decorated decellularized cornea as 91 full thickness stromal substitute

Sindhuja 3D Fabrication of Perfusion Based Bioreactor 93 S. and Subha to Investigate Cell Survival in 3D Bioprinted Blood Vessels

vii

Inkjet printing of metallic biomaterial, process 94 physics and strength reliability 3D inkjet powder printing of UHMWPE: A 95 correlated study of 3D microstructure and mechanical properties

DAY 1: POSTER Sl no

Label

Ano

Authors

Title

Page No

Theme: 3D printing 1

3DP-1

6

Sudipto Datta, Ranjit Barua, Veena Vyas, Ankita Das, Ananya Barui, Amit Roychowdhury and Pallab Datta

Design and Development of 96 Alginate:Polyglutamic Acid scafflods by 3D bioprinting and studying their Mechanical, Structural and Cell Viability Properties. Aarushi Sharma, Shikha 3D-Bioprinting of Cartilage 96 Chawla and Sourabh Ghosh Shilpa Ajit, Shiny Velayudhan Optimization of Photo crosslinkable 97 and Anil Kumar P.R Bio ink with UV Protective Molecules for 3D Bio printing Amrita Natarajan and Prabha OSTEOCHONDRO MIMETIC 98 D Nair GRADIENT 3D PRINTED BASED CONSTRUCTS FOR ENHANCING TISSUE REGENERATION Rahul V G and Prabha Nair A comparative study on 3D printed 98 and electrospun elastomeric tracheal constructs Venkatesh Vakucherla,. Development of coaxial 3D bone 99 Mohan Varma D S and. Geetha scaffold Manivasagam Asheesh Maheshwari, Tushita Controlling 3D printed Prosthetic 100 Bhattacharya, Soumya Limb and providing Tactile feedback Ranjan, Snehashish Paul, using Biologically Inspired Textile Gyanendrai Panigrah, Gwen Force Sensor Millett and Neha Sinha Jaivignesh M, Suresh Babu A In vitro Biocompatibility Study of 100 and Arumaikkannu G Additive Manufactured Titanium Cellular Structures for Mandibular Implants Theme: Orthopedic

2

3DP-2

64

3

3DP-3

171

4

3DP-4

174

5

3DP-5

186

6

3DP-6

268

7

3DP-7

276

8

3DP-9

136

9

ORT-2

203

Bargavi P, Chitra S, Combination of nanostructured 101 Durgalakshmi D, Rajashree P Bioglass-Alumina composite thin films and Balakumar S for Orthopaedic – Joint replacement implants applications Theme: Biomaterials

10

BMAT-1

37

11

BMAT-2

79

12

BMAT-3

141

13

BMAT-4

154

Selvakumar Gopika, Kuttalam Synthesis and Characterization of Iyappan and Lonchin Suguna Polydatin Embedded PullulanCollagen Scaffold for Tissue Repair and Regeneration Socrates Radhakrishnan, Mineralized silicon incorporated native Sakthivel Nagarajan, Mikhael fibrillar collagen matrix for biomedical Bechelany and Narayana application Kalkura Subbaraya Eva C Das, Anil Kumar P R, Synthetic Osteogenic Matrix using Jisha Babu, Sameer Dhavan, Polymeric Dendritic Peptides for Kumary T V, Haridas V and treating Human Periodontal defects – Manoj Komath design and in vitro evaluation. Suruchi Poddar, Piyush Sunil Stabilization and Characterization of Agarwal, Ajay Kumar Sahi, Psyllium Husk (Isabgol) Scaffolds for

viii

102

103

104

104

Kiran Yellappa Vajanthri and Wound Care Applications Sanjeev Kumar Mahto 14

BMAT-5

155

15

BMAT-6

162

16

BMAT-7

179

Chinchu K Sabu, Prabha D Nair and Lynda V Thomas

17

BMAT-8

181

Purva Gupta, Syed Muntazir Andrabi and Ashok Kumar

18

BMAT-9

182

Adarsh R K and Dr. Manoj Komath

19

BMAT-10

13

20

BMAT-11

197

21

BMAT-12

93

Chetna Verma, Deepak Pathania, Poonam Negi and Bhuvanesh Gupta Souvik Ghosh, Ananya Shrivastava, Dr. Debrupa Lahiri, Pallavi Jha, Partha Roy and Pallavi Gupta Radhakrishnan Vidya

22

BMAT-13

228

23

BMAT-14

235

24

BMAT-15

284

Poonam R. Inamdar1*, Parixit Terpyridine based copper complexes: 113 Bhandurge1, A. Sheela2* Protein binding agents as biomimetics

25

BMAT-16

121

26

BMAT-21

76

27

BMAT-24

145

28

BMAT-26

213

Dhivyaa Anandan, Sonal S, Sourangshu Chakraborty and Amit Kumar Jaiswal V Sharathkumar Reddy, Jyoteesh G, Pearlin Hamwed, Subrahmanyam J and Geetha Manivasagam Mamatha M Pillai, Sathishkumar G, Shadi Houshyar, Rajiv Padhye and Amitava Bhattacharyya Dhivyaa Anandan, Manisha Srinivas Raghavan, Prerana Sensharma, Arunai Nambiraj N and Amit Kumar Jaiswal

Neelima Varshney, Ajay Kumar Sahi and Sanjeev Kumar Mahto Catherine Martin, Subathra Radhakrishnan and Narayana Kalkura

Fabrication of Macroporous Three dimensional PDMS Scaffold For Tissue Engineering Applications Improving Safety of Major Liver Surgery by augmentation of Liver function using Biocompatible Scaffolds with Hepatic Progenitor Cells A novel chitosan-xanthan gum/Polycaprolactone layered scaffold for cartilage tissue engineering Polymeric composite cryogels for adsorption of toxic ammonia from blood during acute liver failure conditions Graded membranes of quarternised chitosan with nano calcium phosphate filler for periodontal tissue regeneration - Preparation and properties. Smart Biomaterial by graft functionalization of Tragacanth Gum

105

Comparative Analysis of Neural Cell Behavior on Anisotropic Polymeric Scaffolds Reinforced with Aligned Carbon Nanofillers Toxicology of Biomaterials Through Food-chain in Ranipet Vellore districtTamilnadu Vidya,R.,VIT University,Vellore Devlina Ghosh, Mugdha Crosslinking of Poly-vinyl alcohol and Joglekar, Dhivyaa Anandan Gum based Scaffolds: A comparison and Amit Kumar Jaiswal between Glutaraldehyde and Sodium trimetaphosphate Brindha J, Kaushik Chanda and A single pot method for Protein based Balamurali Mm biomaterial generation

110

ix

106

107

108

108

109

111

111

112

Development of acemannan containing 114 composite scaffolds for bone tissue engineering applications Development of bioactive Titanium 114 composite foam for orthopedic applications Nanocomposite coated silk fibroin 115 based conducting 3D braided scaffolds for peripheral nerve tissue engineering Gum based 3d composite scaffolds for 116 bone tissue engineering applications

29

BMAT-28

30

---

G.Priya1, R.Anitha2, R.Akila2, A preliminary study towards 116 U.Narendra Kumar3 and investigation of bacterial interaction I.Manjubala1* with the composite scaffolds Theme: Antimicrobial Biomaterials

AMB-1

30

Emmanuel Joseph, Amrita Patil, Swarali Hirlekar, Abhijit Shete, Anuya Nisal and Asmita Prabhune

31

AMB-2

156

32

AMB-3

168

33

SE-1

29

34

SE-2

44

35

SE-3

69

36

SE-4

183

37

SE-5

220

38

SE-6

240

39

SE-7

250

40

SE-8

293

41

SE-16

114

Functional biocompatible implant 117 coatings based on Silk fibroin and glycomonoterpene exhibiting improved crack resistance, excellent anti-biofilm and quorum quenching activity Manohara Dhulappa Jalageri, Design and antimicrobial activity of 118 Yashoda Malgar Puttaiahgowda piperazine polymer nanocomposites and Hariprasad Shetty Akshatha Nagaraja and M. P Environmental friendly polymer 119 Yashoda nanocomposite against pathogenic microorganisms Theme: Surface Engineering Shazia Shaikh, Sunita Kedia, Deepti Singh, Ananda Guha Majumdar, Mahesh Subramanian and Sucharita Sinha Aleena Mary Cherian, Manitha B Nair, Divya Suresh, Shantikumar V Nair, Vijayakumar M and Deepthy Menon Gopinath Perumal, Amrita Chakrabarti, Aditya Ayyagari, Dr. Sundeep Mukherjee, Dr. Harpreet Singh Arora, Dr. Harpreet Singh Grewal, Dr. Soumya Pati and Dr. Shailja Singh Jay Panji, Balaji Ramachandran, Hemalatha Kanniyappan, Sudip Chakraborty and Vignesh Muthuvijayan Gobi Saravanan Kaliaraj and Vinita Vishwakarma

Yubraj Lamichhane, Gurpreet Singh, Amandeep Singh Bhui, Purushottam Kumar, Prabin Kumar Mukhiya and Bikram Thapa Jesslyn Kim Ean Tan, Geetha Manivasagam and Poovarasi Balan Ashwini K Patil1, D.Davidson Jebaseelan2, Geetha Manivasagam3 Pearlin Hameed, Vasanth Gopal, Stefan Bjorklund, Ashish

x

Nanosecond Laser Surface Treatment 119 of Ti6Al4V Bio-alloy for Improved Performance

Stable titania nanotexturing on metal coronary stent surface improved endothelialization reduced smooth muscle proliferation The influence of phase change grain refinement in SS316L biomedical applications

bare 120 for and cell and 121 for

Surface Modifications for Enhancing 122 the Biocompatibility of PET

Biological and electrochemical 122 behavior of Zirconia modified 316L SS for Potential Biomedical Applications Surface modification of AISI 316L 123 Stainless Steel with HAp nanoparticles using PMEDM for enhanced biocompatibility Surface modifications of Mg-based 124 biomedical applications: A review Surface Morphology studies of pre- 124 sintered Dental Yttria Stabilized Zirconia Axial Suspension Plasma Spraying: A 125 Potential Alternate Approach For

Ganvir, Dwaipayan Sen, Conventional Air Plasma Spraying Of Nicolaie Markocsan and Geetha Hap To Enhance Osteointegration Of Manivasagam Ti Implants. Theme: Tissue Engineering 42

TE-1

275

Neelima Thottappillil Prabha Damodaran Nair

and Dual source electrospun scaffold facilitates differentiation of adipose stem cells to smooth muscle cells for vascular tissue engineering Gopal Agarwal and Akshay Electro-conductive collagen-graphene Srivastava hydrogel conduit for spinal cord regeneration Somnath Maji, Tarun Extracellular matrix functionalized Agarwal and Tapas Maiti PAMAM based hetero-macroporous scaffold for enhanced differentiation of human mesenchymal stem cell microtissue: Potential for bone tissue engineering application. Pathmanapan Srinivetha and Fibrin Hydrogel Incorporated with Anandasadagopan Sureshkumar Graphene Oxide Functionalized Superparamagnetic NanoHydroxyapatite as Scaffolds for Bone Repair – In vitro and In vivo Study Kalyani E, Siva Sankari M, Silver Sulfadiazine Incorporated Rajan Vk, Dr Raja Biswas and Human Adipose Derived Decellularized Extracellular MatrixDr R Jayakumar Chitosan Hydrogel Bandage For Burn Wound Healing and Infectious Condition Ankush Dewle, Heta Thakar GAG modified Iso-electrically and Dr. Akshay Srivastava Focused Align Collagen Type-I patch for Annulus Fibrosus Repair and Regeneration. Vineeth C A, Annie John, Fabrication of decellularised scaffold Josna Joseph and Annie from rabbit Achilles tendon – a natural substitute to repair damaged tendon. Abraham Rebu Sundar, Annie Preliminary screening of bioceramics Abraham, Josna Joseph, for bone tissue engineering Suresh Babu, H.K Varma and applications Annie John Sagar Nilawar and Kaushik Poly(ester amide)s Based On Olive Oil Chatterjee for Tissue Engineering

126

43

TE-2

32

44

TE-3

35

45

TE-4

38

46

TE-5

42

47

TE-6

70

48

TE-7

88

49

TE-8

102

50

TE-9

105

51

TE-10

138

Narmadha Rajeshwaran, Elakkiya Venugopal and Selvakumar Rajendran Aadinath W and Vignesh Muthuvijayan

133

52

TE-11

163

53

TE-12

209

Udhaya Kumar, Chellappa Muralidharan, Siddan Gowthaman, Madurai Sugunalaxmi and Chellan Rose

54

TE-13

137

Elakkiya Venugopal, Amitava Bhattacharyya and Selvakumar Rajendran

xi

Development of chitosan based thermoreversible hydrogel for tissue engineering application Stimuli-responsive Pickering emulsion-templated porous scaffolds for tissue engineering applications Fabrication of Curcumin-TiO2 Nanoparticle Functionalized CollagenMontmorillonite Nanocomposite as Anti-Microbial Matrix for Wound Healing: an in vitro and in vivo investigation Bone and cartilage tissue engineering using electrospun PCL nanofibers blended with active phytochemicals from Wattakaka volubilis

127

128

129

129

130

131

132

132

134

135

136

55

TE-16

20

56

TE-17

33

57

BTE-1

54

58

BTE-2

61

59

BTE-3

67

60

BTE-4

61

Tarun Agarwal, Aruja Rustagi Functionalization of filter paper matrix 136 and Tapas Maiti with mouse hepatocyte-derived extracellular matrix for development of 3D human liver model for drug screening applications Geeta Kumari Wasupalli and Hydrothermally treated polyelectrolyte 137 Devendra Verma complex scaffolds for bone tissue engineering Theme: Bone Tissue Engineering Biolmed Innovations, Raeesa Sayyad, Rucha Deshpande, Shalm Radhakrishnan Salunke, Anuya Nisal and Premanath Venugopalan N. Vijay Kumar, Raghav Soni, Shibu Chameettachal, Falguni Pati and Subha Narayan Rath Asif Ali and Yuvraj Singh Negi

In-vivo and In-vitro studies on 138 SeriossTM, a novel osteoconductive Silk Fibroin based bone graft substitute

118

N Valarmathi, N Arunai Nambi Raj and S Sumathi

140

BTE-5

170

62

BTE-6

196

Grace Felciya S J, Giriprasath Ramanathan and Uma Tiruchirapalli Sivagnanam Jissy Joseph, Arun .A. Rauf and Boby.T. Edwin

63

BTE-7

222

64

BTE-8

246

65

BTE-9

260

66

BTE-10

272

67

BTE-11

41

68

WH-1

16

69

WH-2

26

Rutusmita Mishra, Ritu Varshney, Neeladrisingha Das, Dr. Debabrata Sircar and Prof. Partha Roy Sneha Gupta, Parvaiz Ahmad Shiekh and Ashok Kumar Kaul

Synthesis and Optimization of PCLBioactive Glass Composite Scaffold for bone Tissue Engineering Effect of carbon based fillers on properties of Chitosan/PVA/β-TCP based composite scaffold for bone tissue engineering In vitro biological performance of Copper-hydroxyapatite/chitosan/ polyvinyl pyrrolidone composite for bone tissue engineering Biodegradable cellulose-based biomaterial for Bone Tissue Regeneration Antioxidant property of biomaterial scaffold for bone tissue engineering

139

139

141

141

Synthesis and characterization of a 142 bioactive polymer hybrid scaffold for bone tissue engineering Antioxidant based polymer scaffold 143 with oxygen releasing properties in combination with bone substitutes for treating critical sized bone defects Fucoidan-Based Composite Beads for 144 Bone Tissue Repair and Regeneration

Venkatesan Jayachandran, Sukumaran Anil and Sudha P.N Yashodhan Ektare, Anup Kale, Biocompatibility of PAEK- 144 Vedashree Sirdeshmukh, Kunal Hydroxyapatite Nanocomposites for Tillu and Ishan Mahabal Bone Implantation Krishanu Ghosal, Upama Municipal Plastic Waste Derived 145 Bhattacharjee and Kishor Biopolymer for Bone-Cartilage Tissue Regeneration Sarkar Theme: Wound Healing Preethi Jenifer and Fabricated approach for an effective 146 Mangathayaru Kalachaveedu wound dressing material using a Natural Gum impregnated with Acalypha indica extract Rajalekshmy G P and Dr. Physico-chemical Characterization of 147 Rekha M R Alginate Based Wound Care Material for Chronic wound Management

xii

70

WH-3

180

Vimala Devi Mohan, Shiny Pj Electrospun nanofibrous construct 147 and Uma Tiruchirapalli augmented with bacitracin-layered Sivagnanam double hydroxides for burn wound healing Shiny John, Vimala Devi A dual- functioning therapeutic 148 Mohan and Uma Ts dressing for burn wound management

71

WH-4

187

72

WH-5

279

73

WH-6

74

WH-7

POOJA R1, KETANKUMAR Synthesis of bacterial cellulose and 149 VADODARIA2 AND herbal extract for the development of VIDHYA.S*1 wound dressing R. Mala, A. S. Ruby Celsia and Multi functional nano fibrous mat for 149 V.Varalakshmi diabetic wound healing Saranya Sugumar1*, Nanoemulsion loaded thin film 150 Ramamurthy Nagarajan1, Joyce development with plant based oils Nirmala1, Natarajan against wound causing Chandrasekaran2 microorganisms Theme: BioEngineering

75

BENG-1

159

Juna Konikkara

76

WC-1

78

Renji Rajendran and V Raj

77

WC-2

271

78

WC-3

188

79

CER-1

149

80

CER-2

211

81

CER-3

214

82

CER-4

243

83

CER-5

244

84

CER-6

256

Advanced bandages to stop 150 haemorrhage from the lower limb extremities Theme: Wear and Corrosion

Fabrication of TiO2mineral 151 substituted fha/biopolymer coatings with enhanced mechanical, antibacterial and corrosion resistance properties T V V L N Rao, Ahmad Majdi Squeeze Film Analysis of Three- 152 Abdul Rani and Geetha layered Parallel Plate and Partial Manivasagam Journal Bearing Lubricated with Couple Stress Fluids for Skeletal Joint Applications Satish Jaiswal, Shubham Effect of Multi Axial Hot Forging on 153 Agrawal and Debrupa Lahiri Corrosion resistance, Mechanical and Biocompatibility Behaviour of Mg3Zn Alloy Theme: Ceramic Nishad K V, Eva C Das, Manoj Calcium-strontium-silicate composite 153 Komath and Unnikrishnan G cement for endodontic repair – design and in vitro studies Abhijit Vyas, Arnav Kaushal, Synthesis of Novel Modified 154 Sanchita Bandyopadhyay-Ghosh Fluorcanasite based Glass-ceramics for and Subrata Bandhu Ghosh Biomedical Application. Chitra S, Bargavi P, Rajashree Insight on Calcium Fluoride reinforced 155 P and Balakumar S Bioglass for Dentin Remineralization Sumit Das Lala, Ashish B. Deoghare, Payel Deb, Emon Barua and Sushovan Chatterjee Payel Deb, Ashish B. Deoghare, Emon Barua and Sumit Das Lala G Vidhya and E.K. Girija

xiii

Characterization of hydroxyapatite 156 derived from eggshells for medical implants Synthesis of hydroxyapatite from 156 Labeo rohita fish scale for biomedical application Comparative study of hydroxyapatite 157 prepared from eggshells and synthetic precursors by microwave irradiation method for medical applications

85

CER-8

146

M Mohan Babu

86

HYG-1

19

Ravindra Badhe

87

HYG-2

43

88

HYG-3

51

89

HYG-4

52

90

HYG-5

55

91

HYG-6

85

92

HYG-7

89

93

HYG-8

192

94

HYG-9

205

95

HYG-10

252

96

HYG-11

15

97

HYG-12

17

The Effect of ZrO2 doped bioactive 158 glass structural properties of ZnONa2O-CaO-P2O5 bioglass system analysis Theme: Hydrogel

Formulation and Development of 159 Chitosan–Gelatin Composite Biomaterial Based Hydrogel for Wound Healing Ramanathan Yegappan, Injectable Angiogenic and Osteogenic 159 Vignesh Selvaprithiviraj, Stimulating Porous Carrageenan Nanocomposite Hydrogel Sivashanmugam Amirthalingam, Annapoorna Mohandas and Jayakumar Rangasamy Shiva Muthuswamy, Vignesh Injectable Antibacterial and 160 Selvaprithiviraj, Aparna Neutrophilic Attractant ĸ-Carrageenan Viswanathan, Jayakumar Hydrogel for Efficient Wound Healing Rangasamy and Raja Biswas Rituparna Saha and Prakriti Development of cost-effective 161 Tayalia phytochemical incorporated gelatinchitosan gels for skin tissue regeneration Aathira Pradeep, M Adhesive, Hemostatic and 162 Nivedhitha Sundaram, Raja Antibacterial in-situ Gel for Preventing Biswas, Praveen Varma and Bleeding and Controlling Infections Jayakumar Rangasamy after Cardiac Surgery Santosh Kumar B Y, G C Physico-mechanical Properties of 163 Mohan Kumar and Arun M Cuttlebone Derived Hydroxyapatite Isloor Loaded PVA Hydrogel Implants for Articular Cartilages Kapender Phogat and Sanchita Novel Bio-nanocomposite Hydrogel as 164 Bandyopadhyay-Ghosh Injectable Bone Scaffold Vinita Dhaware, Sayam Sen Biopolymer/ PPO-Galactose Blended Gupta and David Diaz Diaz Hydrogels for Liver Tissue Engineering Debabrata Nayak, Subrata Development of Novel Injectable Bandhu Ghosh and Sanchita Nanocomposite Hydrogel for Bone Bandyopadhyay-Ghosh Tissue Engineering Functionalization of silk-fibroin Privita Edwina Ra G exhibits improved spreading of fibroblasts over 2D hydrogel Vimal Rohan K, Bavya M C Injectable Nanoformulation Based and Rohit Srivastava Hydrogel for Sepsis Management Anurup Mukhopadhyay, Monika Rajput, Ananya Barui, Pallab Datta, Shiv Sekhar Chatterjee, Nishith Kumar Pal, Rabibrata Mukherjee and Jyotirmoy Chatterjee

xiv

165

165

166

167

Honey Embedded Dual Crosslinked 168 Sodium Alginate Hydrogel for Cutaneous Wound Healing Application

DAY 2: POSTER Sl No.

Label

A.No

Authors

Title

Page No

Theme: Biomechanics 1

BMECH-1

18

Ajithkumar V and Sharmila Nageswaran

2

BMECH-2

130

V Chaithanya Vinay and Mohan Varma D S

3

BMECH-3

161

4

BMECH-4

184

Usha Rani Thirunavukkarasu, Snekhalatha Umapathy, Ramji Kalidoss, Ravi Chandran Thirunavukkarasu and Kumar J.S Sakthivel Sankaran

5

BMECH-5

219

Rohit Goyal

6

BMECH-6

249

Prashanth R Kubasad and Dr. Somasekhara Rao Todeti

7

BMECH-7

282

8

BMECH-8

255

Archana.R1, Manish Baldia2, Devakumar2, J.B.Jeeva1, Arokiaraj Xavier1, Mathew Joseph2 Varun Sharma, Nenad Grujovic, Fatima Zivic, Dragan Adamovic and Bogdan Vasiljević (International)

Computational Analysis of Suitable 170 Material for the Substitution of Nucleus Pulposus in Lumbar Region Simulation studies of auxetic liner for 170 prosthetic limb application Thermal Imaging in Plantar Region: 171 An Assessment Tool for Early Detection of Foot Complications in Type II Diabetes Mellitus

Design of stability controlled reciprocating gait prosthesis module for above knee amputees Soft Actuation Technologies in Robotics and Challenges Review on mechanisms of ankle foot orthosis to improve gait of individuals with foot drop Strength analysis of Cranioplasty flap materials

171

172 173

173

Numerical modeling of porous PMMA 173 bone cement under compressive loading

Theme: Biosensor 9

BSEN-1

160

Ramji Kalidoss, Snekhalatha Umapathy and Usha Rani

10

BSEN-2

263

11

BSEN-3

285

Nivedha S, Ramesh Babu P and Senthilnathan K A.l.G.N.Aditya1, B.Jones2, Elizabeth Rufus3

12

BSEN-4

262

Melwin G, Ramesh Babu P and Senthilnathan K

13

BSEN-5

265

Gandhi C, Ramesh Babu P and Senthilnathan K

14

CR-1

40

S Rajkumar and M Prabaharan

Diagnosis of Diabetes Mellitus via 175 Feature Extraction of Exhaled Breath Print by means of an array of Graphene based gas sensors Nearly D shaped biosensor using a 175 photonic crystal fiber. Surface Cell Adhesion on Active 176 Substrates for Bio-sensors - A future step for Single Cell Capture D shaped biosensor with MoS2 as a 176 sensing layer using a conventional telecommunication optical fiber Designing a biosensor using a 177 metamaterial absorber

Theme: Cancer Research Multifunctional core-shell gold 177 nanoparticles as a theranostic agent for cancer therapy

xv

15

CR-2

108

Nalinee Kanth Kadiyala, Badal Kumar Mandal, Dwaipayan Sen and L Vinod Kumar Reddy

16

CR-3

47

Debjani Ghosh, Krishanu Ghosal and Kishor Sarkar

17

CR-4

158

18

CR-5

193

Balashanmugam Pannerselvam, Sucharithra Ganesh, Tamilselvi Alagumuthu, Agnes Mary S and Swarna V Kanth Jaya Thilakan, Sudhir Goel and Neha Arya

19

CR-6

238

20

CR-7

113

21

CR-8

254

22

CT-1

165

Raunak Kumar Das, Sourangshu Chakraborty, Christopher Ngan, Mikaela Cicciarello and Noel Whitaker

Induction of Epithelio- mesenchymal 183 transition (EMT) changes by tumour viruses

23

CT-2

233

Rajavenkatesh K, Aishwarya S, Purna Sai K and Thennarasu S

24

CT-3

57

Lekshmi Krishna, Dr. Murugeshwari Ponnalagu and Dr. Debashish Das

Design and synthesis of N-terminal 184 Labeled undecapeptide: a case study towards the targeting cancer cell Decellularized human amniotic 184 membrane exhibits protective role under oxidative stress to maintain the

Sivasubramanian Murugappan, Shrikant Kirwale, Venkatesh Pooladanda, Sowjanya Thatikonda and Chandraiah Godugu Reetoja Nag, Mousumi Pal, Ranjan Paul, Jyotirmoy Chatterjee and Raunak Das Ripon Sarkar, Kabita Chatterjee and Ananya Barui

One pot green synthesised Zinc Oxide 178 nanoparticles decorated reduced graphene oxide nanocomposite (ZnONPs-rGO-NC) as advanced materials for novel anticancer theranostics Green Synthesis of Carbon 179 Dot@Silver Nanoparticles: A Potential Nanocomposite for Breast Cancer Theranostics Preparation, Characterisation and 179 Anticancer activity of PVA and PCL based Electrospun Nanofibers

In situ mineralization of collagen- 180 based hydrogels as an in vitro breast cancer model SeNPs Induce Autophagic Mediated 181 Apoptotic Cell Death in Human Keratinocytes

Chaos and loss of information during 182 progression of oral cancer Gas Phase Cigarette Smoke Extract 182 Alters Cell Morphology and Intracellular Properties of Primary Oral Keratinocyte

Theme: Cell Therapy

cell functionality of cultured ARPE-19 cells.

25

CT-4

80

Josna Joseph and Annie Abraham

26

CT-5

164

Aswathy M.R, Dr.Roy Joseph and Dr.Praveen K S

Extracellular matrix based three 185 dimensional cell culture systems for in situ cytocompatibility evaluation of select phytochemicals Heat shock protein 70 mediated 186 myoblast proliferation and differentiation on alginate dialdehyde -gelatin hydrogel system

Theme: Gene Therapy

xvi

27

CT-7

48

Rima Saha, Rapti Chakraborty and Kishor Sarkar

Reversible Addition-Fragmentation 187 chain Transfer (RAFT) Polymerization Mediated Cationic Poly(amino acid) for Efficient Gene Carrier

Theme: Drug Delivery 28

DDEL-1

36

Smrutirekha Mishra and Leena Nebhani

29

DDEL-2

84

Ifra Mirza and Sampa Saha

30

DDEL-3

9

Bavya M C, Vimal Rohan K and Rohit Srivastava

31

DDEL-4

65

32

DDEL-5

166

Piyush Gupta, Sreenath Pappuru, Santosh Gupta, Debashis Chakraborty and Rama Verma Renju Radhakrishnan and Kaladhar Kamalasanan

33

DDEL-6

178

34

DDEL-7

189

35

DDEL-8

201

Gayathri P P and Dr. Vidhya Viswanad

36

DDEL-9

71

Ayan Chatterjee, Srijeet Tripathy, Milan Sasmal, Tapas K Maiti and Tarun K Bhattacharyya

37

DDEL-10

106

Abhishek Sinha and Suresh Palamadai Krishnan

38

DDEL-11

140

Vothani Sarath Babu, Chittoor Pallavi and Kedam Thyagaraju

39

DDEL-12

269

Dhanaja Pillai and Swati Gupta

40

DDEL-13

283

S. Kalaiarasan1, Sk Ershadul Haque2 Angappan Sheela1*

41

DDEL-14

73

Ahmaduddin Khan, Amit Kumar Jaiswal and Niroj Kumar Sahu

Krishnapriya M, Vyshma K.V, Megha Hansen and Kaladhar Kamalasanan Joseph Jose and Vignesh Muthuvijayan

xvii

Synthesis and Characterization of Stimuli Responsive Polymer Grafted Organically Modified Mesoporous Silica and its Application in Targeted Drug Delivery Polymer brushes grown cup shaped particle via surface initiated ATRP for triggered drug delivery Nanoengineered dual release graft for pain and inflammation management in osteoarthritis Enhanced anti-cancerous activity of dual-drug loaded core-shell nanoparticles composed of metal-free fully alternating copolymer Degradation of gelatin in various acid conditions and analysis of components

188

188

189

190

191

Foating drug delivery system using 191 Diclofenac sodium as a model drug A novel and simple technique for separation of liposomes from unloaded drug molecules Formulation and evaluation of betamethasone dipropionate loaded emulgel for cutaneous lupus erythematosus Zinc oxide nanoparticle conjugated peptide delivery to mature murine dendritic cells tested by microfluidic single cell level pairing with T lymphocytes A Comparative Study of Nano-Stealth Delivery Systems for Luteolin to Mediate Caspase-3 Induction in HaCaT cells Formulation development and in-vitro characterization of biodegradable Nanoparticles of rosuvastatin drug by using solvent evaporation method. Brinzolamide -2 Hydroxypropyl Beta Cyclodextrin Inclusion Complex loaded Chitosan Nanogels for Ocular Delivery Polymeric Blend Microparticles for Sustained Release of Metformin

192

193

193

194

195

196

197

pH Labile PEGylated Mesoporous 198 Magnetite Nanocluster for Drug Delivery and Hyperthermia Applications

42

DDEL-15

---

Andrew Ebenazer, Jonathan Sampath Franklyne, Nisha Tiwari, Prakash Amruth Raj .Ch, Amitava Mukherjee, N. Chandrasekaran ([email protected])

Study on extended drug release of 199 Itraconazole in chitosan coated thyme oil based Microemulsion

Theme: Nanotechnology 43

NTECH-1

31

Krishna Kumari Swain and Sunil Bhand

44

NTECH-2

87

Gayathri Sundar, Josna Joseph, Annie John and Annie Abraham

45

NTECH-3

111

Thanusu Parandhaman and Sujoy K Das

46

NTECH-4

147

Hemalatha Kanniyappan and Vignesh Muthuvijayan

47

NTECH-5

150

48

NTECH-6

195

49

NTECH-7

210

Ramya Kannan, Vignesh Muthuvijayan and Edamana Prasad Tharun Selvam Mahendran, Arkajyoti Sarkar, Rushenka Vashti Christopher, Shreya Srinivasan, Shreya Natarajan, Aasha M and Sahabudeen S Mayank Bhushan and Yogesh Kumar

50

NTECH-8

7

Radhika Poojari, Rohit Srivastava and Dulal Panda

51

NTECH-9

167

M. Joyce Nirmala and Ramamurthy Nagarajan

52

NTECH10

199

Varun Saxena and Lalit Pandey

53

NTECH11

223

54

NTECH12

224

Neeladrisingha Das, Suparna Saha, Lokesh Kumar, Sandip Nathani, Subhashish Samantaray, Debabrata Sircar, Parimi Suresh and Partha Roy Sruthi Alex, Debolina Chakraborty, T. Monisha Priya, Natarajan

xviii

A Colorimetric magnetic nano particle 199 based enzyme assay for sensitive detection of Cd(II) ions in drinking water. Phytofabrication and characterization 200 of silver nanoparticles using Mirabilis jalapa tuber extract for Skin Burns Effect of Multifunctional GrapheneSilver Nanocomposite on Antibacterial and Anti-biofouling Material and Toxicity Profile in Zebrafish Synthesis and characterization of mesoporous silica nanoparticles (MSNs) embedded polymeric hydrogels (PH-MSNs) for drug delivery applications Mechanistic study on the antibacterial activity of lower generation selfassembled amphiphilic dendrimers Neuroprotective Properties of Cerium Oxide Nanoparticles against Bisphenol-A Induced Autism Spectrum Disorder - related Symptoms in Drosophila Melanogaster

201

202

202

203

Study of Antibacterial Applications of 204 Iron/Manganese Oxide Nanocomposites along with their Structural, Magnetic and Cytotoxic Characteristics Molecular Intersection of a 3-in-1 205 Nanomedicine Targeting Microtubules, ERK Tyrosine Kinases with Profound Nuclear Modulations, and Quantum Imaging for Hepatocellular Carcinoma Therapy Development of Cumin Oil 205 Nanoemulsion and its Applications Antibacterial mechanism of Fe(III) 206 doped ZnO nanoantibiotic under dark conditions Anti-diabetic effect of Trivanga 207 bhasma - An ancient Indian Nanomedicine

Folic acid-conjugated gold nanorods 208 for cisplatin loading, their release profiles, and potential drug delivery

Chandrasekaran, Ramshankar Vijayalakshmi and Amitava Mukherjee

application

Zinc Oxides nanorods deposited 208 cellulose sheet for antimicrobial activity In vitro release and cytotoxic studies 209 of aliginate nanocarrier for the antitumor drug: sunitinib Hyperthermic, antimicrobial and in- 210 vitro biocompatibility studies on synthesized cobalt ferrite nanoparticles for biomedical applications.

55

NTECH13

234

Priyank Bhutiya and Nirendra Misra

56

NTECH14

237

57

NTECH15

264

58

NTECH16

277

59

NTECH17

291

60

NTECH18

46

D. Sangeetha Thanaraj, Jjohnjoseph Jayapal and Shiva Shankar M Lickmichand M, Crystal Sara Shaji, Valarmathi N, Amy Sarah Benjamin, R K Arun Kumar, Sunita Nayak, Radha Saraswathy, Sumathi S and Arunai Nambi Raj N Sapna Rangarajan, Vidya R, Geetanjali Chimote, Shilpa Verekar, Sunil Deshmukh, Arun Balakrishnan, Somesh Sharma and Jayesh Bellare Siripireddy Balaji1, Badal Kumar Mandal1,*, Dwaipayan Sen2, L.Vinod Kumar Reddy2 Pratik Das and Kishor Sarkar

61

NTECH19

267

Amy Sarah Benjamin, Arunai Nambi Raj and Sunita Nayak

Evaluation of Antibacterial efficacy 210 and biofilm disruption potential of silver nanoparticles synthesized from novel strain of Flammulina velutipes Biogenic Ceria nanoparticles (CeO2 211 NPs) for Photocatalytic and Cytotoxic activity One-pot Facile Green Synthesis of 212 Gelatin Functionalized Silver Nanoparticle for Bimodal Targeted Therapy of Hepatocellular Carcinoma Synthesis and Characterization of 212 Genepin Cross-linked Gelatin Coated Magnetic Nanoparticles for Hyperthermic Therapy

Theme: Theraputics 62

THER-1

266

Venkatesan K, Dr Jomon Raphael C, Dr Mathew Varghese K, Dr Paul Gopu G, Sivakumar P, Minu Boban, Dr Arunai Nambi Raj N, Dr Senthilnathan K and Dr Ramesh Babu P

Radiomics analysis of MRI images in 213 Astrocytoma and glioma patients

Theme: Stem Cell 63

STMC-1

215

Erfath Thanjeem Begum Moghal and Dwaipayan Sen

64

STMC-2

216

Erfath Thanjeem Begum Moghal and Dwaipayan Sen

65

STMC-3

217

Moghal Erfath Thanjeem Begum, Himadri Shekhaar Baul, Venkatesh Katari and Dwaipayan Sen

xix

DOR agonist (SNC-80) exhibits anti- 215 parkinsonian effect via downregulating UPR/oxidative stress signals and inflammatory response in vivo Effect of DOR activation on crosstalk 215 between oxidative stress by the inhibition of PARP-1 and inflammatory response in vitro model of PD The novel miRNAs regulated with 216 DOR activation against ER stress in an in vitro model of Parkinson’s disease (PD).

66

STMC-4

226

67

STMC-5

99

68

STMC-6

153

69

STMC-7

157

70

STMC-8

116

71

STMC-9

120

72

STMC-10

122

Duraimurugan Muniswami and Dwaipayan Sen

73

STMC-11

175

Jijo Wilson J and Prabha D Nair

74

STMC-12

191

75

STMC-13

253

Suchandra Bagchi, Amrita Chaudhary, Anurup Mukhopadhyay, Palash Dhara, Monika Rajput, Ananya Barui, Rabibrata Mukherjee and Jyotirmoy Chatterjee Ankita Das, Ripon Sarkar and Ananya Barui

76

STMC-14

134

Madhubanti Mullick and Dwaipayan Sen

77

STMC-16

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Venkatesh Katari, Pearlin Hameed, Ankita Parida, Shounak Ghosh and Dwaipayan Sen

Madhubanti Mullick, Pearlin Hameed, Moghal Erfath Thanjeem Begum and Dwaipayan Sen Santhosh Kacham, Sreenivasa Rao Parcha and Sachin Shukla Subathra Radhakrishnan, Shanmugaapriya Sellathamby, Catherine Ann Martin, Mettu Srinivas Reddy and Mohammed Rela Priyanka Sarkar and Kavitha Thirumurugan Pearlin Hameed, Geetha Manivasagam and Dr. Dwaipayan Sen L Vinod Kumar Reddy and Dwaipayan Sen

Amelioration of Acetaminophen- 217 induced liver injury in vivo using delta-opioid receptor activated human mesenchymal stem cells Invitro transdifferentiation of human 217 Umbilical Cord Mesenchymal Stem Cells (hUC-MSC) into corneal/limbal epithelial cells. Nucleostemin a prognostic marker of 218 impaired stemness during in vitro expansion of Infrapatellar fat pad derived stem cells RNF213-a plausible link between 219 adipogenesis and angiogenesis Small molecule mediated osteogenic 220 of human Mesenchymal stem cells for segmental bone defects. Activation of Hu-MSCs with DOR 221 agonist SNC-80 can promote cardiomyocyte differentiation, angiogenesis and anti-inflammation Secretome of human umbilical cord 221 stem cells - therapeutic effects Three dimensional polymeric matrices 222 encapsulating mesenchymal stem cells for augmenting the differentiation in to Islet like clusters. Nanopatterned Honey Silk Fibroin 223 Matrix Provides Mechanical Clues for Mesenchymal Stem Cells

Biocompatibility of electrospun PVA- 224 honey nanofibers on umbilical cord derived mesenchymal stem cells. The delta opioid peptide DADLE 224 represses hypoxia-reperfusion mimicked stress mediated apoptotic cell death in human mesenchymal stem cells in part by downregulating the unfolded protein response and ROS along with enhanced antiinflammatory effect. Design and development of three- 225 dimensional human Neural stem cells using natural hydrogels

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Abstracts Prof. Abhay Pandit Professor, Director Network of Excellence for Functional Biomaterials (NFB) National University of Ireland Galway E-mail: [email protected] Professor Abhay Pandit is the Established Professor in Biomaterials. He is the Director of a Science Foundation Ireland funded Centre for Research in Medical Devices (CÚRAM) at the the National University of Ireland, Galway. He obtained a PhD from the University of Alabama at Birmingham, where his postgraduate work focussed on the modification of a fibrin scaffold to deliver a therapeutic biomolecule and resulted in a clinical trial at the Burn Centre. Prof Pandit has over twenty-five years of experience in the field of biomaterials. After a seven-year stint in industry he has worked in academia for the last twelve years. His research is funded by Science Foundation Ireland, the 7th EU Framework programme, Enterprise Ireland, Health Research Board, the AO Foundation and industry sources, and in excess of €78 million. He is the author of 4 patents and has licensed three technologies to medical device companies. He has published more than 180 manuscripts in high-impact factor publications. He was inducted as an International Fellow in Biomaterials Science and Engineering by the International Union of Societies for Biomaterials Science and Engineering (IUSBSE). He has been elected as a Council Member of the Tissue Engineering and Regenerative Medicine and the International Society and European Society for Biomaterials. Redefining identity of disease, tissues and cells – a Biomaterials paradigm Abhay Pandit CÚRAM, Center for Research in Medical Devices, National University of Ireland, Galway, Ireland [email protected] Abstract: Biomaterials are no longer considered innate structures and using functionalisation and biofabrication strategies to modulate a desired response whether it is a host or implant is currently an important focus in current research paradigms. Fundamentally, a thorough understanding the host response will enable us to design proper functionalisation and biofabrication strategies. The input from the host response needs to be weighed in depending on the host disease condition. In addition, biomaterials themselves provide immense therapeutic benefits which needs to be accounted in the design paradigm. Using functionalisation strategies such as enzymatic and hyperbranched linking systems, we have been able to link biomolecules to different structural moieties. The programmed assembly of biomolecules into higher-order selforganized systems is central to innumerable biological processes and development of the next generation of biofabricated scaffolds. Recent design efforts have utilized a developmental biology approach toward both understanding and engineering supramolecular protein assemblies. Structural moieties have taken a variety of different forms such as nanofibers and nanoparticulate. This approach has resulted in functionalisation of micro and nanoparticles with biomolecules that include designed peptide motifs, growth factors and a multitude of gene vector systems. In addition, nature itself has abundant structural complexity that can be biofabricated for harnessing in key targeted clinical applications. This talk will elucidate some of these ongoing strategies in our laboratory. 1

Amit Bandyopadhyay, Ph.D. Herman and Brita Lindholm Endowed Chair Professor Fellow WSAS, NAI, AAAS, ASM International, AIMBE and ACerS School of Mechanical and Materials Engineering Washington State University, Pullman, WA 99164-2920 E-mail: [email protected] Amit Bandyopadhyay is a Herman and Brita Lindholm Endowed Chair Professor in the School of Mechanical and Materials Engineering (MME) at Washington State University. His research expertise lies with 3D Printing or additive manufacturing of metallic and ceramic materials and their composites towards structural, bio- and piezoelectric applications. Prof. Bandyopadhyay published over 300 technical papers including over 200 journal papers, inventor of 17 issued patents, and edited 10 books. His research papers have been cited over 13250 times by various research groups and his current “h” index is 62. Prof. Bandyopadhyay supervised / supervising 18 Ph.D. and 30 MS students in Mechanical Engineering, Materials Science and Engineering, and Physics. Among others, Prof. Bandyopadhyay received the CAREER award from the US National Science Foundation, and the Young Investigator Program (YIP) Award from the US Office of Naval Research. Prof. Bandyopadhyay is a Fellow of the American Ceramic Society (ACerS), American Society for Materials (ASM International), American Institute for Medical and Biological Engineering (AIMBE), American Association for the Advancement of Science (AAAS) and National Academy of Inventors (NAI). In 2017, he was elected to the Washington State Academy of Sciences.

3D Printing of Hard Materials for Structural and Biomedical Applications Amit Bandyopadhyay School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920 E-mail: [email protected] Abstract: 3D Printing (3DP) is an approach to process parts directly from its computer aided design (CAD) file. 3DP is changing the landscapes of current industrial practices. On-demand manufacturing using 3DP technologies is a new trend that will significantly influence many industries and product design protocols. Since there is no need for any part specific tooling, different parts can be built using the same machine. Most of these parts are net-shape or near netshape that may or may not require any finishing operation. We have worked on 3DP of hard materials, primarily metals and ceramics, over the last two decades. We have manufactured parts with compositional, functional and structural gradation mostly for space and biomedical applications. My presentation will focus some of the key success stories from our research, as well as current challenges in the field.

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Prof. Maurizio Vedani Full Professor Chief of the Material Section a Department of Mechanical Engineering Email maurizio.vedani(at)polimi.it Expertise • Microstructure and mechanical behaviour during manufacturing and service of several metals and metallic alloys Awards and honors • Authored or co-authored more than 240 scientific papers • Co-ordinates the Scientific Board of the Department of Mechanical Engineering • Deputy Director of the same department • President of the "Physical metallurgy and materials science” Technical Committee of the Italian Association for Metallurgy • President of the “Light Metals” Committee in the same association since 2013-end Advanced metallic alloys and modern processing techniques in the biodegradable material field” Maurizio Vedani Politecnico di Milano, Dept. of Mechanical Engineering (Italy) Abstract: In the contribution, a brief summary of recent achievement obtained at Politecnico di Milano in the field of innovative biodegradable metals and of related processing will be given. The topics covered span from an overview of the fundamental physics principles used to improve strength of metals, to the effects of elements on biocompatibility and corrosion behavior, up to the most advanced metal-forming processes such as Equal Channel angular pressing and additive manufacturing and their opportunities for the development of customized products. Results will particularly be focused on Magnesium alloys processed by different methods and on innovative Zinc alloys.

Nicholas Dunne Dublin City University, Dublin, Ireland Email- [email protected] Background: Professor Nicholas Dunne is the Chair of Mechanical and Manufacturing Engineering in the School of Mechanical and Manufacturing and the Director of the Medical Engineering Research Centre Engineering (MedEng) at DCU. Professor Dunne is also a Visiting Research Professor of Biomaterials Engineering at the School of Pharmacy at Queen's University of Belfast, an Adjunct Professor of the School of Mechanical Engineering at Trinity College Dublin and Principal Investigator in the Trinity Centre for Bioengineering. Expertise: materials science, engineering and biology Awards and honors Orthopaedic Research Society/British Orthopaedic Research Society Fellowship (2008) and the RAEng/Leverhulme Trust Senior Research Fellow Award (2010) 3

Calcium Phosphate Based Biomaterials for Bone Tissue Engineering Applications Nicholas J Dunne1-3 1. Centre for Medical Engineering Research, School of Mechanical and Manufacturing Engineering, Dublin City University, Stokes Building, Collins Avenue, Dublin 9, Ireland 2. School of Pharmacy, Queen’s University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK 3. Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland [email protected] Abstract: Currently, strong needs in orthopaedics have still not been fulfilled, in terms of bone and joint substitution or in the repair and regeneration of bone defects following disease or traumatic injury. Notwithstanding this fact, significant developments in the biomaterials field have been made over the last half century - where materials intended for biomedical applications have progressed through three different generations, namely bioinert materials (first generation), bioactive and biodegradable materials (second generation) and materials purposely designed to accelerate specific stimuli or responses at a molecular level (third generation). This paper will focus on the interdisciplinary work being conducted in our laboratory in the Medical Engineering Research Centre at Dublin City University - relating to the development and in vitro/ in vivo characterisation of calcium phosphate based biomaterials (i.e. injectable cements, 3D printed scaffolds and stimuli responsive ceramic-polymer gels) for bone tissue engineering applications, as well as the presentation of the different approaches that we are using to meet the challenges faced by calcium phosphate based biomaterials within orthopaedics.

Bhuvanesh Gupta Indian Institute of Technology Delhi Email: [email protected] Bhuvanesh Gupta is the professor of Polymer in the Department of Textile Technology at Indian Institute of Technology, New Delhi. Prof. Gupta heads the Bioengineering group in the department. The major interests of the group are in polymer functionalization, biomaterials, hydrogels, biocompatible structures, nanomaterials and tissue engineering. The diversity of the group is in international collaborations in the field of bioengineering and biomaterials. The bio activities are well supported by Indian and European funding agencies. Bhuvanesh Gupta is associated with Indian Institute of Technology Delhi since 1996. He is guiding a group of scientists and students dedicated to the field of polymeric biomaterials and bioengineering. After a post-doctoral assignment at Paris, senior scientist assignment in Switzerland, he was associated with different laboratories in Europe. He has been member of several professional societies along with the President of Asian Polymer Association, Society of Biomaterials & Artificial organs, India-North and has been collaborating with several laboratories in India and Abroad.

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Engineering of Biopolymers for Smart Healthcare Bhuvanesh Gupta and Chetna Verma Bioengineering Laboratory, Department of Textile Technology, Indian Institute of Technology, New Delhi-110016, India. Email: [email protected] Abstract: Hydrogels and hydrocolloids have been an advantage to human healthcare due to their very innovative and beneficial life support features. Hydrogels have excellent biocompatibility, hydrophilicity, processability and sometimes they are bioactive in nature as well. A series of hydrogels based on biopolymers, such as chitosan, pectin and dextran have shown enormous potential in wound care system. These hydrogels may be combined with appropriate ingredients to develop a system which would offer optimum healing to the wound. Herbal bioactive agents have been used for this application. However, the development of such materials requires a close collaboration between medicine, microbiology and materials chemistry. The development of polymers with bioactive coating is an important area of research focused on solving the problem of contamination by infection in wound care systems. We have observed that a bioactive component may be incorporated within the hydrogel matrix to make it infection resistant. Herbal drugs and essential oils have been incorporated into either natural hydrogels like chitosan, pectin and dextran by blending approach. A wide range of natural bioactive agents such as aloe vera, curcumin, sandal wood oil, clove oil and honey are available to develop excellent materials for wound care. These dressings have been evaluated in wound healing using mouse as the animal model. Excellent healing with minimum scar by hydrogel dressings has been observed. References 1. S. Anjum, A. Arora, MS Alam, and B. Gupta, Int J Pharm, 2016, 92, 508. 2. S. Anjum, A. Gupta, D. Sharma, A. Kapil, A. Sharma, and B. Gupta, Mat. Sci. Eng. C. 2016, 64, 157.

Targeting DDR kinases: Novel therapeutics towards cancer treatment Sivapriya Kirubakaran Chemistry & Bioengineering , IIT-Gandhinagar, Palaj, Gujarat-382355, India [email protected] Abstract: Cancer is considered to be a major killer in recent times worldwide. One of the causes is the deregulation of the kinase a primary mechanism by which cancer cells evade normal physiological constraints on the growth and survival. Such aberrant functions of the kinases in a cancer cell have highlighted them as one of the most successful families of drug targets. Innovative approaches in chemical biology have played a key role in validating the importance of kinases as molecular targets. However, the detailed understanding of the protein structure and the mechanisms of protein–drug interaction through biochemical and biophysical techniques demands a method for the production of an active protein of exceptional stability and purity on a large scale. We will present the recent developments in the discovery of small molecules towards the most important kinases that are part of DNA damage and repair (DDR) pathway such as ATR, ATM and TLK developed in our lab. The talk would include the importance of interdiciplinary science as an overall aim.

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Prof. Diego Mantovani Professor, Department of Mining, Metallurgy and Materials Engineering Director, Biomaterials and Bioengineering laboratory Laval University, Canada Email: [email protected] Website : http: // www .lbb.gmn.ulaval.ca Holder of the Canada Research Chair in Biomaterials and Bioengineering for the Innovation in Surgery, professor at the Department of Materials Engineering at Laval University, adjunct director at the Division of Regenerative Medicine of the Research Center of the CHU de Québec, Diego Mantovani is a recognised specialist in biomaterials. At the frontier between engineering, medicine and biology, within his team, their works aim to improve the clinical performances of medical devices for functional replacement, and to envisage the next generations of biomaterials to develop artificial organs enhancing the quality of the life of patients. He has authored more than 260 original articles, holds 4 patents, and presented more than 120 keynotes, invited and seminar lectures worldwide in the field of advanced materials for biomedical applications. His H-Factor is 42 (May 2018) and his works received more than 6600 citations. In 2012, he was nominated Fellow of the International Union of Societies for Biomaterials Science & Engineering (FBSE) for his leadership and contribution to biomaterials for medical devices. He was Executive Co-Chair of the 10th World Biomaterials Congress 2016. He is advisor of three medical devices consortium in the Americas, Asia and Europe.

3D triple cell culture in bioreactors from collagen gel scaffolds: innovation in advanced in vitro models, cosmetics, and regenerative medicine Diego Mantovani Lab for Biomaterials and Bioengineering, Canada Research Chair Tier I for the Innovation in Surgery Dept of Min-Met-Materials Engineering & CHU de Québec Research Center Laval University, Québec City, Canada [email protected]; www.lbb.ulaval.ca

Abstract: Over the last 50 years, biomaterials, prostheses and implants saved and prolonged the life of millions of humans around the globe. Today, nano-biotechnology, nanomaterials and surface modifications provides a new insight to the current problem of biomaterial complications, and even allows us to envisage strategies for the organ shortage. In this talk, creative strategies for mixing vascular cells and collagen-based materials will be targeted with the overall aim to envisage today how far innovation can bring tomorrow solutions for regenerative medicine. Collagen gel is a commonly used scaffold in vascular tissue engineering due to its biological properties including a high potential for supporting and guiding vascular cells in the regeneration process. With the aim to regenerate the vascular wall, the approach we deployed consisted in first reproducing the media, which provide the high elastic properties of the vessel wall, thus making it an essential and effective component for blood and nutrients transportation. Starting 6

from an original method aimed to process collagen and smooth muscle cells (SMCs), we developed an endothelialised two layers collagen cell-based tubular scaffold. The external layer was composed of fibroblasts (FBs) and SMCs seeded within collagen. The middle layer was composed of SMCs seeded within collagen, and endothelial cells (ECs) were culture on the lumen of the construct. The construct was expected to provide vascular tissue remodeling due to cells/cells and cells/matrix interactions and to produce an engineered tissue with hierarchical structure close to that of blood vessel walls. It was also expected to provide a valid in vitro model for further studies of vascular patho-physiology. The middle and external layer were mold around a mandrel, directly in the bioreactor chamber. Then, the mandrel was removed and a ECs solution was perfused inside the lumen. The interaction between cells enhanced the matrix remodeling and the properties of the arterial construct resulted strongly improved. This shows that vascular cells tri-culture using collagen gel scaffold is a valid strategy for the regeneration of the vascular tissue. The overall take home message of this talk is aimed to show how 3D pluri-culture of appropriate material/cell/environment represent the today bottleneck in regenerative medicine and which are few of the strategies that have to be investigated to push forward innovation in the field.

Prof. Arun K. Kota Assistant Professor, Dept. of Mechanical Engineering, Colorado State University (CSU), USA Email: [email protected] Prof. Arun K. Kota received a B.S. in Chemical Engineering from Andhra University in 2001, M.S. in Chemical Engineering from Clarkson University in 2003, and Ph.D. in Mechanical Engineering from the University of Maryland in 2008. Prior to joining Colorado State University in 2013, he was a Postdoctoral Fellow in Materials Science and Engineering at the University of Michigan. His work has so far resulted in 6 patents and 30+ publications, which are cited 2100+ times. His work was highlighted by Bloomberg TV, NBC News, Wall Street Journal, Washington Times and several other prestigious newspapers, magazines and websites. He received the NSF Career Award (2018), Best Science Paper Award from the Institution of Civil Engineers (2014), the Teaching Excellence Award from Colorado State University (2016) and the Summer Faculty Fellowship Award from the Air Force Office of Scientific Research (2016).

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Superomniphobic Surfaces: Design & Applications Arun K. Kota Department of Mechanical Engineering Department of Chemical and Biological Engineering School of Biomedical Engineering School of Advanced Materials Discovery Colorado State University, Fort Collins, CO Abstract: Recent years have seen significant advances in the understanding of super-repellent surfaces, i.e., surfaces with extreme repellency to liquids. Surfaces with extreme repellency to high surface tension liquids like water are considered to be superhydrophobic. Surfaces with extreme repellency to low surface tension liquids like oils and alcohols are considered to be superoleophobic. Surfaces that are both superhydrophobic and superoleophobic are considered superomniphobic, i.e., extremely repellent to virtually any liquid. In this presentation, the fundamental chemical and physical principles of designing superomniphobic surfaces will be discussed. Based on these fundamental principles, novel applications of superomniphobic surfaces in chemical shielding, inexpensive surface tension sensors, enhanced hemocompatibility, and enhanced condensation heat transfer will be discussed.

Michael Tatoulian Chimie ParisTech, PSL Research University, CNRS, Institut de Recherche de Chimie Paris, 75005 Paris, France Email : michael‐[email protected] Michael Tatoulian received the Ph.D. degree in chemical engineering from Pierre et Marie Curie University (UPMC)–Paris 6 (Paris, France). Michael Tatoulian is currently full professor in chemical engineering at Chimie Paristech, and director of the LGPPTS (Laboratory of Plasma processing and surface treatment (LGPPTS) since 2012. He is the author of 105 scientific papers (48 in International refereed journals -62 in conference proceedings), 2 patents, 1 keynote, 2 plenary presentations and 24 invited talks, (10 in international conferences, 14 in laboratory or university seminars). His group is specialized in the surface modifications of different materials (polymers, glass, steel…) by plasma technology. In particular, the “plasma team” has developed in the last ten years, lot of work in the plasma polymerization of organic and inorganic coatings, in low pressure or atmospheric pressure systems, enlarging the fields of applications of the plasma technology. Since 2005, Prof. M. Tatoulian developed specific collaborations to enlarge the application of plasma technology to the development and fabrication of microsystems ; (i) with Dr. Patrick Tabeling (ESPCI) to control the synthesis of o/w/o emulsions in microfluidic devices, (ii) with Prof. D. Bonn (ENS) for the development of catalytic microreactors aiming to provide fundamental insight in their reaction mechanism, and (iii) with Prof. E. Dufour-Gergam (IEFParis Sud) to develop microfabrication technology ("MEMS Process By Film Transfer Using Fluorocarbon Anti-adhesive Layer").

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Plasma Processing for surface modification : Biomedical Applications C. Guyon and M. Tatoulian Chimie ParisTech, PSL Research University, CNRS, Institut de Recherche de Chimie Paris, 75005 Paris, France [email protected] Abstract: Plasma processes started to be applied for surface modification of materials in the 70’s, in the fields of microelectronics and semiconductors. Since then, enormous advancements in the basic, diagnostic and experimental aspects of plasma sciences have been made, so that many other science areas and industrial fields have been permeated by plasma processes: polymers, textiles, biomaterials, microfluidics, composite materials, paper, packaging, automobile, waste treatment, to mention but a few. More specifically for biomaterials, many applications such as the production of biosensors, protein immobilization, improved cell adhesion or improved biocompatibility require the presence of specific surface chemical functions. The advantages of plasma lie in the ability to generate active species that are chemically and physically actives (excited atomic, molecular, ionic and radical species) at low temperature. We begin with a brief overview of the physics and chemistry of cold plasmas. Next, interaction mechanisms between plasma and a material will be examined: these include the 3 main effects, namely, (i) cleaning/ablation, (ii) modification of surface-chemical structure, and (iii) plasma enhanced chemical vapour deposition (PECVD). In this presentation, we will report recent advances in the successful use of low pressure plasmas for surface modifications and thin film growth. Considering the actual environmental constraints, emphasis will also be put on the atmospheric pressure plasma jet process, as a promising technology for surface treatments. Keywords: Plasma functionalization, surface modifications, thin film coatings, atmospheric pressure plasma jet

Ketul C Popat Associate Professor School of Biomedical Engineering, Colorado State University, USA Email: [email protected] Dr. Popat is an Associate Professor in the Department of Mechanical Engineering/School of Biomedical Engineering at Colorado State University. Prior to that, he was working as a Research Specialist in the Department of Physiology at University of California, San Francisco. He has authored over 85 peer-reviewed publications in journals such as Langmuir, Biomaterials, Journal of Orthopedic Research, Journal of Biomedical Materials Research, etc. and has and hindex of 37. He has also presented his work at numerous national and international level conferences. He received his Ph.D. in Bioengineering from University of Illinois at Chicago in 2003, M.S. in Chemical Engineering from Illinois Institute of Technology, Chicago in 2000 and B.E. in Chemical Engineering from M. S. University in India in 1998.

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Micro/Nano-engineering of material surfaces for tissue engineering and regenerative medicine Ketul C. Popat Associate Professor Biomaterials and Surface Micro/Nano-engineering Laboratory Department of Mechanical Engineering/School of Biomedical Engineering Colorado State University, Fort Collins CO Abstract: Surfaces that contain micro- and nanoscale features in a well-controlled and “engineered” manner have been shown to significantly affect cellular and subcellular function of various biological systems. Our research is focused towards using the tools of micro- and nanotechnology for application in biomaterials and tissue engineering. The goal of current research is to design implants that induce controlled, guided, and rapid healing. In addition to acceleration of normal wound healing phenomena, these implants should result in the formation of a characteristic interfacial layer with adequate biomechanical properties. To achieve these goals, however, a better understanding of events at the tissue-material interface is needed, as well as the development of new materials and approaches that promote biointegration. Our work proposes the use of well-controlled nanostructured interfaces to enhance implant integration. We hypothesize that controlled nanoscale architectures can promote cell differentiation and matrix production, and enhance short-term and long-term integration. Moreover, the ability to create model nano-dimensional constructs that mimics physiological systems can aid in studying complex tissue interactions in terms of cell communication, response to matrix geometry, and effect of external chemical stimuli. By understanding how physical surface parameters influence cellular adhesion and differentiation, we can more effectively design biomaterial interfaces that can be used in a clinical setting.

Dr. Yasuharu Ohgoe Associate Professor Faculty of Science and Engineering Department of Science Tokyo Denki University Email: [email protected] Background:

Electronic and mechanical Engineering

Expertise: Biomaterials, Surface modification, Plasma processing, DLC (Diamond-like Carbon) films Awards and honors • Tokyo Denki University Yujiro Niwa Award • Best Title Award at the 34th Annual Artificial Heart and Auxiliary Circulation Council • IEEE MGA GOLD (Graduates of the Last Decade) Award • IEEE R 10 GOLD (Graduates of the Last Decade) Award

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Effects of plasma treatment and foreign element contents on a-C:H film depositions for biomedical applications Yasuharu Ohgoe Tokyo Denki University, Japan Abstract: Hydrogenated amorphous carbon (a-C:H) including diamond-like carbon has outstanding properties such as hardness, low friction, and chemical inertness for preventive corrosion. Many kinds of a-C:H film coatings can be produced by various deposition techniques, and recent trials of the coatings for medical devices have indicated that they are promising for blood interfacing applications such as artificial heart valves, blood pumps, and stents are already commercially available. Additionally, a-C:H film has been also expected to be a biological compatible material for potential cell culture matrix and bio-medical applications. However, it is not easy to decide properties of the a-C:H films and optimize deposition method for each performance on biomedical applications [1]. It is well known that among the many possible ways to modify biological properties of a-C:H films, surface modification using plasma treatment and incorporation of foreign elements during film deposition have shown to be especially powerful. In our research works, we have focused on cell and blood compatibility of a-C:H films as a biological responses. In order to optimize the film properties for cell behavior, osteogenesis, and hemolysis as a biological performance, a-C:H film surface were changed by oxygen plasma treatment or added nitrogen [2], zinc [3], fluorine [4] as a foreign element. The results indicate that with the oxygen plasma treatment and the nitrogen contents, the cell adhesion and proliferation was enhanced. Additionally, the results suggested the possibility that osteogenesis could be enhanced by the Zn that is released from Zn-DLC. On the other hand, the results indicate that with the fluorine contents, the surface energy decreases, while the hemolytic performance tends to improvement. The effects of the plasma treatment and the foreign element contents on the a-C:H film depositions and biocompatible properties of the film the performance will be discussed at the conference. Reference 1.

2.

Y. Ohgoe, K. K. Hirakuri, H. Saitoh, T. Nakahigashi, N. Ohtake, A. Hirata, K. Kanda, M. Hiratsuka, Y. Fukui, “Classification of DLC films in terms of biological response”, Surface & Coatings Technology Vol. 207 No. 1, 350-354, 2012. Y. Ohgoe, T. Wada, Y. Shiraishi, H. Miura, K. K. Hirakuri, A. Funakubo, T. Yambe, and Y. Fukui, “Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and invitro tests”, Mater. Res. Soc. Symp. Proc. (2012 MRS Fall Meeting) Vol. 1498, mrsf12-1498-l09-30, 2013.

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Franck Clement Associate Professor, University of Pau, Pau, France Email: [email protected]

Background: • Ph.D. (Plasma Physics), University of Pau Expertise: • Plasma Chemistry • Dielectric Barrier Discharge • Plasma Medicine • Atmospheric Plasma Cold Atmospheric Plasmas for biomedical applications. Afterglows and Ionization Waves in Biology and Medicine. Franck Clément Pau University – UPPA, France Abstract: An increasing use of Cold Atmospheric Plasmas (CAPs) is realized in biology and medicine since twenty years. Numerous applications are developed around the world as for instance sterilization, decontamination, hemostasis, wound healing and cancerology. This presentation will be focused on demonstrations of how specific electrical discharges in gases allow to develop CAP technologies at atmospheric pressure and temperatures closed to the room one, thus well adapted for biological applications. More specifically two processes will be described. The first one, called « afterglow », is issued from plasma reactors from which electrical charges are removed. Reactivity is transferred to long-lived reactive species, which are transferred on biological substrates by the propagation of hydrodynamic waves. The second system, called « ionization wave », produce electromagnetic waves containing sufficient energies for gas ionization, during and along their propagation. This way, plasmas with electrical charges can be transferred directly on biological substrates. In all cases, CAPs contain various energy components as electrical charges (electrons, ions), photons (UV-visible), Reactive Species (as Reactive Oxygen and Nitrogen Species: ROS, RNS). It is important to note that thermal and electrical field effects may occur. For that the user of such processes has to specifically control the energy distribution of these gases for an optimization of the applicative development. Based on state-of-the-art of the biomedical applications developed around the world, we will describe these both technologies. Comparisons of the different energy sources will be realized in order to identify the main components, which are involved in such devices. Particular attention will be done on electrical fields and Reactive Oxygen and Nitrogen Species. Methods and techniques used for these characterizations will be presented. The multidisciplinary approach combines physical, chemical and biological methods that operate at different timescales, which must be taken into account for the analysis of CAPs interactions on living organisms. The necessity to work with specialists in many fields will be shown trough our studies conducted over the last ten years.

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Dr Kaushik Chatterjee Associate Professor, Department of Materials Engineering Indian Institute of Science Email: kchatterjee[AT]iisc.ac.in Background: Metallurgical Engineering, Bioengineering. Expertise: Biomaterials; Biomedical engineering; Tissue engineering; Regenerative medicine; Cancer; Stem cells; Medical implants. Awards and honors: • • • •

DAE/BRNS: Young Scientist Research Award 2012 ACS Combinatorial Science (Journal): Young Investigator Panel Member 2012 DST: Ramanujan Fellowship 2011 – ’16 NIST: Distinguished Research Associate Award 2010

Engineering multi-biofunctional materials for orthopaedic applications Kaushik Chatterjee Department of Materials Engineering, Indian Institute of Science [email protected] Abstract: Disorders of the bone resulting from disease and trauma are a major disease burden. The current strategies of autografts and allografts suffer from several limitations. This has motivated the development of biomaterials for replacement and regeneration of the bone tissue. In our group, we work on engineering materials for use in biomedical devices and as scaffolds for bone tissue regeneration. We have developed several nanoscale surface engineering techniques to improve the performance of titanium and its alloys for use in orthopaedic devices. In one strategy, we have developed nanoscale pillars inspired by insect wings that are bactercidal and yet compatible with human stem cells. In another approach, we induce surface nanocrystallization through severe surface deformation that enhances the fatigue properties and also the cellular response to these materials. Toward preparing scaffolds for bone tissue engineering, we are exploring the use of nanoparticle for improving the bioactivity of polymers. We have demonstrated that graphene can fuctionalized to impart a combination of osteogenic, angiogenic and bactericidal activity to the polymer composite. In a different approach, we have prepared nanocomposite polymer fibers incorporating ceramic nanoparticles that elute bioactive ions. Taken together, we have developed several strategies to impart multifunctional properties to materials for use in biomedical applications.

Susmita Bose Herman and Brita Lindholm Endowed Chair Professor School of mechanical and materials engineering, School of Mechanical and Materials Engineering Washington State University Email: [email protected] Prof. Bose has interdisciplinary research interests in the areas of chemistry, biology, materials science and engineering, involving calcium phosphate based bioceramics, surface modification of load bearing implants, 3-D printing of bone tissue engineering scaffolds, drug delivery, in 13

vitro and in vivo bone cell material interaction study. Prof. Bose is the editorial board member of several international journals, including Additive Manufacturing, Acta Biomaterialia, Journal of the American Ceramic Society, International Journal of Nanomedicine. Clinical Significance of 3D Printing in Bone Health Susmita Bose School of mechanical and materials engineering, School of Mechanical and Materials Engineering, Washington State University Abstract: 3D printing (3DP) or additive manufacturing technology enables us to make devices with controlled geometry / architecture and chemistry depending on clinical needs. Patients with special anatomical needs or defect complexity, patient matched devices are necessary sometimes, where 3DP can play a significant role to make multifunctional biomedical devices. There are an estimated one million bone grafting procedures performed annually in the U.S. and a few million worldwide to repair fractures, craniomaxillofacial defects, bone defects, tumors, as well as hip and knee replacements due to the aging population, increase in musculoskeletal disorders and sports related injuries. 3DP resorbable calcium phosphate (CaP) ceramics, a bone like material, show significant promise towards bone tissue engineering and surface modified metallic load bearing hip knee devices. Dopant chemistry plays a vital role in controlling their mechanical strength, degradation kinetics, as well as biological properties. 3D interconnected channels provide pathways for micronutrients, improved tissue – material interactions, osteogenesis and angiogenesis with these 3DP scaffolds. Chemistry modification can help with different protein expressions which are responsible for improved biological performance, as well as infection control. It is important to realize 3DP in healthcare would involve visualization and planning, personalization, functional integration and manufacturing economics. The presentation will include recent scientific and technological advances as well as challenges towards developing next generation biomedical devices using 3DP for bone disorders. It is worth noting that the convergence of different disciplines from academic researchers to physicians and industry facilitation are needed to effectively use 3DP technology in health.

Bikramjit Basu Professor Materials Research Centre, Indian Institute of Science (IISc), Bangalore E-mail: [email protected] Dr. Bikramjit Basu is currently a Professor at the Materials Research Center and holds Associate Faculty position at Center for Biosystems Science and Engineering, Indian Institute of Science (IISc), Bangalore. After his undergraduate and postgraduate degree in Metallurgical Engineering, he earned his PhD in Ceramics at Katholieke Universiteit Leuven, Belgium in March, 2001. Following a brief post-doctoral stint at University of California, Santa Barbara; he joined IIT Kanpur in November 2001 as Assistant Professor and remained there till May, 2011, when he moved to IISc. While on lien at IISc, he was promoted to the rank of full Professor at IIT Kanpur in March, 2012. Prof. Basu’s contributions in Engineering Science have been widely recognised. He is one of the youngest recipients from the Metallurgy/Materials Science community as well as the only Biomaterial Scientist in the complete history of India’s most coveted science award, Shanti Swarup Bhatnagar award (2013). He remains the only Indian from India to receive the prestigious ‘Coble Award for Young Scholars’ (2008) from the American Ceramic Society. He 14

is an elected Fellow of the American Institute of Medical and Biological Engineering (2017), Indian National Academy of Engineering (2015), Society for Biomaterials and Artificial Organs (2014) and National Academy of Sciences, India (2013). He is on the advisory board of several National R & D labs and medical device manufacturing companies in India. Thinking beyond ‘Black-Box’: Quantitative Process Physics of 3D Inkjet Powder Printing and adaptability to bioceramic scaffolds Unveiled Srimanta Barui and Bikramjit Basu* Materials Research Centre and Center for BioSystems Science and Engineering and Translational Center of Excellence on Biomaterials for Orthopedic and Dental Applications Indian Institute of Science, Bangalore 560012, India. Abstract: Over the last few decades, significant attempts were largely directed to re-create functional musculoskeletal systems with considerable potential to treat various types of human diseases (e.g. osteoarthritis, osteoporosis), without investing much efforts to quantitatively understand the process physics of 3D inkjet powder printing. In this backdrop, I will present multiscale measurements and analysis to develop understanding in this line. In particular reference to our ongoing multi-institutional center of excellence on biomaterials, I will thereafter present some of our recent results to demonstrate the efficacy of the 3D powder printing to fabricate Zirconia, Calcium Phosphate and magnesium phosphate-based bioceramic scaffolds. A major emphasis will be placed on the binder formulation, post-processing treatment, and microcomputed tomography of interconnected porous architecture together with the strength relaibility. This presentation will conclude with the speaker’s thoughts on translational research programs and with a few examples of interdisciplinary academic programs to enable young researchers to think laterally, while blending sufficient knowledge of biological systems with engineering sciences to develop next generation biomedical materials. To illustrate this aspect, one of the most recent translational attempts to treat decompressive craniectomy using 3D powder printed patient-specific cranial prosthesis will be presented.

Current trends and Ireland's Activities on 3D printing of BioMaterials Ramesh Raghavendra SEAM Centre Director, Waterford Institute of Technology, Waterford, Ireland Abstract: 3D printing (also known as Additive Manufacturing) has evolved tremendously over the last decade as a disruptive way of manufacturing in a wide range of industries for varying applications. Today nearly all facets of human life from biomedical to aerospace, automotive to construction, food to fashion industry have all been directly affected by 3D printing as they research to exploit this technology to meet their individual needs. The 3D printing process commences with a 3D digital image from a newly generated CAD software design or a digital scan of an existing component. A computer slice is then generated that slices this image into cross sectional layers that are sent to the additive manufacturing equipment that creates the component using a layer upon layer selective deposition technique. What makes 3D printing manufacturing process so disruptive is the design freedom it allows, creating the capacity to manufacture complex components shapes with internal geometries and infrastructure which could not be replicated using conventional subtractive process. Ireland is now taking the lead having embraced the possibilities the 3D printing offers to its manufacturing base with both state and private sectors begun to invest heavily in this area over the last few years. This talk will highlight the key 3D printing technologies and how the sectors that have utilised this technology have witnessed a paradigm shift, especially the health sector. 15

Particular emphasis of the talk will be directed towards Ireland’s focus on Additive Manufacturing and its activities related to 3D printing of biomaterials.

Bala Vidhyanathan Professor of Advanced Materials and Processing Department of Materials, Loughborough University, UK Dr. Bala Vidhyanathan a B.Sc in Physics from Bharathiar University and an M.Sc in Materials Science from PSG College of Technology and a Ph.D in Solid State & Structural Chemistry from Indian Institute of Science, Bangalore, India. He spent three years Post Doctoral Fellowship at the Materials Research Laboratory of the Pennsylvania State University, USA and then joined Loughborough University, UK as a Senior Research Associate. He worked as a Lead-Scientist in General Electric Corporation - Global Research (GE-GRC) between 2004-06 before joining the Department of Materials as a Lecturer in September 2006. His area of research interst is  Functional Ceramics, Nanocrystalline materials, Electroceramics, Microwave processing, Microwave-materials interactions, Flash Sintering  Glasses, Functional coatings, SOFCs  Non-Si Photovoltaics and Hydrogen generation, Ceramics for bio-medical applications  Powder synthesis, Sol-gel methods  Smart materials for sensing, security and sports Fabrication of Nanostructured Biomaterial Implants: The Art of the Possible B. Vaidhyanathan*, K. Annapoorani and Y. Chen Department of Materials, Loughborough University, UK Abstract: The processing of nanocrystalline ceramic powders into useful engineering components has been investigated via a series of research projects each focusing on a different stage of the manufacturing route viz., (i) the ability to control the agglomerates present in the powder resulting in the production of a free-flowing and crushable powder for die pressing, (ii) the formation of a low viscosity but high solids content nano suspensions suitable for slip casting, 2D/3D printing and (iii) the use of novel field assisted sintering techniques. This holistic approach helped to transfer the developments achieved in each stage of the manufacturing process to the next and resulted in the ability to form, for the first time, fully dense, complex ceramic components whilst restricting the grain growth to a minimum. The methodology has been employed to develop various nanostructured zirconia based ceramic components exhibiting vastly superior hydrothermal ageing resistance and mechanical performance suitable for use in biomedical implants (eg., hip/knee prosthesis, finger joints, dental and jaw repairs), surgical tools, petro-chemical valve parts as well as for ballistic armour applications. These novel advancements are covered by a series of patents and papers and this talk will provide an overview of some of these developments. Key Words: nanostructured ceramics, 3D printing, biomedical implants, field assisted sintering.

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ROSALES-IBANEZ RAUL Universidad Nacional Autónoma of México, Mexico Email - [email protected]

Repair of alveolar cleft defect in vietnamese pigs with mesenchymal stem cells and biohybrid scaffolds: A preliminary report Rosales-Ibáñez Raúl Facultad de Estudios Superiores Iztacala, UNAM Tissue Engineering Lab, Mexico City [email protected] Since the concept of tissue engineering was first introduced, researchers have been seeking a solution for bone repair and regeneration, by combining stem cells, growth factors and the use of a porous scaffold1. The design and composition of the scaffold itself, is critical for its ability to promote and allow the cells to adhere, so that they may proliferate and differentiate. Oral derived mesenchymal stem cells for use in tissue engineering approaches, has great potential for solving clinical and surgical problems related to such craniofacial anomalies2. Within the bone defects, we have the craniofacial anomalies as the cleft palate. Clefts, of lip and palate, are congenital deformities of the craniofacial region, with a gap in the upper lip and roof of the mouth. The prevalence of this group of malformations is around 1 in 700 live births in the UK and USA and 1 in 500–700 worldwide3 Cleft palates account for 75% of all craniofacial defects encountered in the US each year, affecting nearly 225,000 children per annum4 According to WHO data, the incidence of cleft lip and palate is 69 out of 10,000 live births around the globe3 In general, the success of surgical treatment is determined by several criteria, such as fistula incidence, hearing problems, maxillary growth, and speech development5. The osteogenic, osteoinductive, and osteoconductive properties of autografts make them the gold standard approach for treating bone defects in cleft palate6. However, autografts do present some limitations: firstly, the graft may fail as a consequence of lack of integration into the host tissue: secondly, donor site morbidity, chronic postoperative pain, hypersensitivity and infection may occur7. In our approach, several materials are used like ceramic, polymers and biocomposites, as well as different sources of stem cells. In that regard, we used dental pulp and palate stem cells from vietnamese pigs and strontium folate (SrFO) derivative was synthesized and loaded into biohybrid scaffolds obtained through lyophilisation of semi-interpenetrating networks of chitosan polyethylene glycol dimethacrylate and beta tri-calcium phosphate (βTCP) fabricated8-9. The objective of this study is to perform bone tissue engineering, using mesenchymal stem cells and a biohydride scaffold with analysis of bone neoformation by means of radiographs, computed tomography and histological techniques. 1.- Yang W, J Biomed Mater Res A. (2015 Jul; 103, 7:2251-9) 2.- Páll E. Microsc Microanal. (2015 Oct; 21, 5:1249-63) 3.- Mossey P. International Collaborative Research on Craniofacial Anomalies, Technical Report 2004, p. 1211.

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Harishkumar Madhyastha Faculty of Applied Physiology, University of Miyazaki, Japan Email: [email protected] Background: • Ph.D. (Applied Bioscience), Sardar Patel University, India • Ph.D. (Medicine), University of Miyazaki, Japan • Msc ( Experimental Biology), Saurastra University • Bsc (Life Science) Mangalore University Expertise: • miRNA mediated wound healing • Arsenic induced skin carcinogenesis Awards and honors • Grantee JRF, UGC, Schweizer Radio und Fernsehen-Council for Scientific and Industrial Research, Government of India. • Member of Japanese Society Agricultural Chemistry and Biotechnology, Japanese Society Physiology, Japanese Society Nepal Association Biotechnology, Indian National Science Academy

Smart Nanocuetical Hybrid in Tissue Resilience during Diabetic Wound healing. Madhyastha Harishkumar*, Madhyastha Radha, Queen Intan Nurrahmah, Eshika Chakraborty, Arjita Ghosh, Raja Sudhakaran, Nakajima Yuichi and Maruyama Masugi Department of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki, 899-1692, JAPAN.  School of Biosciences and Technology, VIT university, Vellore, Tamilnadu, INDIA

BACKGROUND: Skin is the outermost protective layer of the human body, and the largest organ comprising 15% of body weight and 2 m2 surface area. Skin wound healing is an orderly organized process, involving overlapping stages of blood clot formation, inflammation, reepithelialization, granulation tissue formation, neovascularization, and tissue remodeling. On the contrary diabetic wound healing is a complex process with un-orchestrated cellular functions which ultimately delay the healing process. METHODS: The four major types of problematic wounds include ulcers (diabetic, venous, pressure) and burn wounds. Tunable and adjunct healing compound incorporated therapeutic materials which increase angiogenesis, collagen deposition, cell proliferation, and growth factors concentrations, while decreasing inflammation and enzymatic degradation of the extracellular matrix is gaining importance in the field of biomedical sciences. Here we focused on nutraceuticals like curcumin, quercetin and C-pc caged hybrid nano formulations on wound healing processes. Related literature and information were assessed, analyzed and compiled from different scientific sources such as Medline, pubmed and other standard scientific databases. RESULTS:Recent advances in regenerative medicine and nanotechnology are revolutionizing the field of wound healing. Some of the nanocuetical products are used to treat chronic wounds, for example, diabetic foot ulcers, venous leg ulcers, pyoderma gangrenosum, and pressure ulcers. CONCLUSION: The challenges faced and usefulness of the nano-therapies and nanoparticles as pharmaceutical adjuncts will help in treatment of diabetic wounds; however, their optimal use will require future clinical evaluation and human studies. KEYWORDS: Diabetic, wound healing, Nano-technology, healing management. 18

“Fault Lines and Foot Faults” Ankle and Foot Biomechanics in Bipedalism Henry Prakash M Dept of Physical Medicine and Rehabilitation, Christian Medical College Vellore Vellore TN. India [email protected] Abstract: The development of bipedal plantigrade (flat) progression is purely a human accomplishment. We share two legged locomotion with some flightless birds such as an Ostrich, and an arched plantigrade foot with the bears. However, the orthograde (upright) position is exclusively human and permits us to view the world in an upright manner, although not always acting in an upright way! The key processes that drove this evolution took millions of years and was evolved to achieve, a base of support with minimal energetics, to absorb impact forces, store part of these forces and release it for progression. Increase in energy efficiency is also achieved by reducing the excursion of the centre of mass of the whole body. These aspects have brought us a long way. Are we regressing and loosing these advantages to aesthetics!

Dr. S. Kanagaraj Professor Indian Institute of Technology Guwahati [email protected] Background: Mechanical Engineering Expertise: Biomaterials, Carbon nanotubes based nanocomposites, Biomedical devices and implants, Materials characterization, Prosthetic and orthotic devices Awards and honors: • BIRAC-SRISTI GYTI Award 2016 worth of Rs. 1 lakh for fixing the prosthetic knee for 5 amputees • Received Silver Award for Best Innovator at 7th India Innovation Initiative - i3 National Fair 2015 (Recognizing India's top innovator& potential entrepreneurs) organized by DST, AICTE and CII • The prosthetic and orthotic products developed by us were displayed in Make in India exhibition during 13-18th Feb’2016 at BKC, Mumbai • DSIR-PRISM Award Design and development of passive polycentric knee joint and ankle joint for trans-femoral amputees S Kanagaraj Department of Mechanical Engineering, Indian Institute of Technology Guwahati, India [email protected] Abstract: According to World Health Organization (WHO), there are about 30 million amputees currently living in developing countries like Asia, Africa and South America etc. According to the 2011 census, there are 5.4 million locomotor disabled persons in India. It is estimated that around 5-15% of them acquire a prosthetic medical device, and up to 80% of amputees are not able to bear the cost of prosthesis. A knee joint is one of the strongest and most important joints in the human body. It allows the lower leg to move relative to the thigh while supporting weight of the body. Movements at the knee joint are essential to many activities, including walking, running, sitting and standing. The ankle joint provides stability during weight bearing condition and allows the mobility of the foot. The kinematics of ankle include both talocrural hinge joint, 19

which provides planter-flexion and dorsiflexion, and subtalar gliding joint, which provides both inversion and eversion. The above knee amputation is a surgical interference that severs the thigh segment between the hip and knee joint. Though many prosthetic devices are available in the market, which can generate sophisticated and versatile functional joint movements equivalent to that of human lower limb, each one of them has inherent limitations in different aspects. The commercially available prosthetic devices are either expensive or produce many technical problems leading to mismatch of the gait pattern of both sound and prosthetic leg. In order to overcome the same, an attempt is made to design and develop a light weight and user friendly passive artificial limb having prosthetic knee joint and ankle joint with improved functionality with affordability. The knee joint was designed based on the Grashof's criterion, stance phase stability, extension bias and the maximum flexion angle. The polymeric-passive-polycentricprosthetic knee joint having significant advantages over conventional knee joints was designed, developed and trialed with good number of amputees. In case of ankle joint, it was designed to accommodate all kind of its kinematics and the working model of the same having 2 DOF was developed in order to accommodate the prosthetic leg in normal and uneven surfaces.

Figure 1. (a) Patient trial on 1st version of prosthetic knee joint (b) 3rd version of a knee joint (c) Dynamic foot

Bench to bed translational research in Biomaterials– Are we in the right direction? Magesh Sankar, Vasanth Gopal, Jithin Vishnu, Geetha Manivasagam Centre for Biomaterials, Cellular & Molecular Theranostics (CBCMT), VIT, Vellore Abstract: Biomaterials field has unfolded to greater extent in the past 50 years. Nevertheless, researchers have failed to develop an ideal material to meet the real-time difficulties. Earlier, the selection of materials to treat damaged tissues was based on their availability in market. Also, the choice of materials by clinicians is peered towards the strength property of materials for replacements of damaged tissues. Although, the scenario of consecutive implants failure made the researchers, spend a ball of time to develop an ideal material outright with biomedical applications. In spite of the fact, several researchers are working towards the development of ideal material with desirable properties through bulk processing and surface modifications. Yet, all stand in the gap between the translational research and bench work. In this talk, the author will take you through the retrospective current approaches in the field of biomedical research. The answer to the “why?” will be resolved by the ideologies of materials with high ability of antibacterial property and potential remodeling ossification (osteogenesis) processes in area of bone tissue engineering. In accordance with that, the talk will go in hand with the phenomenon of materials up righted with high resistance to biotribological failures for orthopedic applications and medical stents with optimum properties.

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The holy grail of articular cartilage regeneration Manasseh Nithyananth Christian Medical College Vellore, Vellore TN. India Abstract: Osteoarthritis is a widely prevalent disease causing pain and significant disability in older people all over the world. Though joint replacement surgery is available for end stage disease, there are significant side effects and morbidity associated with it. Cartilage regeneration might offer a curative solution for this problem. Many promising advances have been made in the field of cartilage regeneration. Though very widely researched, clinically applicable solution is yet to be found. In this talk, the current status of articular cartilage regeneration and its challenges are explored.

Biopolymeric composites for biomedical and cosmetic applications Alina Sionkowska Department of Chemistry of Biomaterials and Cosmetics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarin 7 street, 87-100 Torun, POLAND email: [email protected] Background: Single biopolymers are widely used in biomedical and cosmetic applications. The new approach is application of the blend of two or even more polymers or biopolymers [1].. Materials based on the blend can be useful in biomedical and cosmetic applications. Methods: Biopolymeric composites were prepared using the blend of three natural polymers, namely collagen, hyaluronic acid and chitosan. Into the biopolymer blend inorganic particles such as iron oxide and hydroxyapatite were incorporated. Thin films and 3D sponges were obtained. The properties of obtained materials were studied using infrared spectroscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Mechanical properties, moisture content as well as porosity and density of 3D matereials were measured. Results: It was found that thin films and 3D sponges made of collagen, hyaluronic acid and chitosan blend with magnetic particles and/or hydroxyapatite have high ability of swelling. Cross-linking process of the materials by chemical cross-linkers increases the swelling degree and alters the mechanical properties. Biopolymeric blends containing magnetic particles show magmetc properties. \Film forming properties of biopolymeric blends are useful in cosmetic preparations. The biological properties of biopolymeric materials were acceptable for biomedical applications. Conclusions: New materials based on the blend of three biopolymers can be potentially used in medical and cosmetic applications. Keywords: Polymer blends, collagen, hyaluronic acid, chitosan, biopolymers, biomaterials, cosmetics. References 1. A. Sionkowska. Prog. Polym. Sci. 2011 ; 36 : 1254. 2. A. Sionkowska, B. Kaczmarek, M. Michalska, K. Lewandowska, S. Grabska. Pure and Applied Chemistry 2017; 89(12): 1829.

Acknowledgements Financial support from the National Science Centre (NCN, Poland) Grant No UMO-2013/11/B/ST8/04444 is gratefully acknowledged. 21

Prof. Ana Paula Rosifini Alves Claro, Universidade Estadual Paulista (UNESP), Brazil E-mail: [email protected] She holds a PhD in Mechanical Engineering from the State University of Campinas (1998), a Master's Degree in Mechanical Engineering from the State University of Campinas (1993) and a degree in Mechanical Engineering, Faculty (1990). She is a Collaborating Professor at Université Laval, Quebec, Canada and Coordinator of the Post-Graduate Program in Mechanical Engineering, Campus de Guaratinguetá. He has experience in the Surface Processing and Treatment of Materials for applications in the Biomedical area, which led to the establishment of international partnerships with Dr Ketul Popat (CSU- Fort Collins, CO, USA), Dr Veronique Migonney (Université Paris 13, Paris, France), Dr Hans J. Griesser (UNISA, Adelaide), Dr Geetha Manivasagam (VIT, India) and Dr Diego Mantovani, Université Laval, Quebec, Canada). Surface Modifications of New Titanium Alloys for Biomedical Applications - Bulk and Surface Properties Alves Claro, A P R 1 1 UNESP – São Paulo State University, School of Engineering, Materials and Technology Department, Guaratinguetá Campus, SP, Brazil Abstract: Since 1960s commercially pure titanium have been used in manufacture of biomedical devices such as dental implants, screws, plates and others. Titanium grade 5 (Ti6Al4V) is quite used, however, the use of vanadium and aluminum in your composition have been contested and attributed some diseases. This resulted in the study of new titanium alloys without cytotoxic elements with better mechanical properties and biocompatibility. Besides, surface properties will be decisive in the success of devices after implantation. When the biomaterial is inserted into the biological environmental a series of events occurs, and some studies have attributed the macro, micro and nano topography to successful response. Several techniques can be used to change these surfaces: mechanical, physical and chemical. Among chemical techniques, the anodic oxidation enables the formation of nanoporous and homogeneous layer on the surface makes the bioactive surface promote differentiation of osteoblasts and bone matrix production. In last years, our group have been studied new titanium alloys and techniques for your functionalization. TiO2 nanotubes were obtained using anodic oxidation on the titanium alloy surfaces associated to incorporation of bacterial agents and drug . Our studies showed that is possible to obtain bacterial coatings successful.

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Sampathkumar T S Professor, Medical Materials Laboratory, Department of Metallurgical & Materials Engineering, Indian Institute of Technology Madras, Chennai Email: [email protected] Dr. T.S. Sampath Kumar is a Professor of Metallurgical and Materials Engineering and head of Medical Materials Laboratory at the Indian Institute of Technology Madras. He received his PhD in materials engineering from Indian Institute of Science, Bangalore in 1986. His current research areas are nanostructured biomaterials, antimicrobial ceramics and delivery systems, injectable bone cements and value added biomaterials from natural wastes. Prof. Sampath Kumar is Fellow, Biomaterials and Artificial Organs (FBAO) and Fellow of the International Medical Sciences Academy (FIMSA). He is presently, President of the Society for tissue engineering and regenerative medicine (India) for 2011-1014 and Vice-President of the Society for Biomaterials and Artificial Organs India (SBAOI) from 2008 onwards. He received the 1994 University Grants Commission Career Award and his research guidance is marked by frequent advisee awards: Sudarsan Bhatt Memorial Awards (2007 & 2009) for best PhD thesis of IIT Madras, best paper awards (Journal of Biomaterials and Tissue Engineering, March 2012; Student paper contest TMS 2011, USA & BIO 2011; International Research Scholars Symposium 2008), best presentation awards (Asian BioCeramics Symposium 2008 India & 2010 Indonesia; ICCS 2010, ISRS 2006, ASTRA 2003 held 1st time in India) and student travel awards (BIO 2011, TMS 2011 USA, Biomaterials-Elsevier 2008 Nepal); Innovation Potential of Students Projects Award 2000 by Indian National Academy of Engineering and Vishwakarma Prize 2012-13 by IIT Madras for undergraduate projects. Prof. Sampath Kumar co-authored a book “Biomaterials: A Nano Approach” published by CRC Press and has recently written a chapter on “Physical and Chemical Characterization of Biomaterials’’ in ‘Characterization of Biomaterials’ published by Elsevier Science & Technology Books. He has published more than 100 papers in peer reviewed journals with many papers having well over 100 citations and edited 3 conference proceedings in journals. He has presented about 150 inaugural address, plenary & keynote talks and invited lectures. He has 3 Indian patents to his credit.

Multifunctional CaP nanocarriers for management of bone infections T.S.Sampath Kumar Medical Materials Laboratory, Department of Metallurgical & Materials Engineering, Indian Institute of Technology Madras, Chennai 600036, India Email: [email protected] Abstract: Bioceramic calcium phosphates (CaP) like hydroxyapatite (HA) resemble natural bone in both structure and chemical composition. CaP nanoparticles, owing to its bioactivity and high surface area has been commonly used as bone substitutes in bone tissue regeneration and as a drug carrier in drug and gene delivery systems. Recent advances in CaP have focused on imparting multifunctionality through ion substitutions in various fields such as bone regeneration, controlled delivery of drugs and medical imaging. In this talk a comprehensive 23

treatment for bone infections based on CaP nanoparticles with features such as combined drug delivery, extended antibacterial activity and multi-mode contrast ability will be presented. The preparation of ion substituted HA through synthetic and natural sources will be explained. Codelivery of antibiotics and anti-inflammatory drugs, biocompatibility and antibacterial cvaluation, and CT/MRI imaging studies of the ion substituted CaP will be discussed. The in vivo animal studies for evaluating the degradation and new bone formation in CaP filled cranial bone defects will also be presented. In addition, the future scope of multifunctional CaP platforms will also be highlighted. Translating Molecular Bioengineering from the Lab to the Patient Ashutosh chilkoti Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA Abstract: This talk will highlight recent work from my laboratory that illustrates the clinical translation of molecular bioengineering technologies for point-of-care clinical diagnostics and drug delivery. In the first example, I will discuss a point-of-care diagnostic —the D4 assay — that we have developed, in which all reagents are printed and stored on a “non-fouling”— protein and cell resistant—polymer brush. The D4 assay has a speed and sensitivity that is as good or better than commercially available point-of-care tests and is far simpler, cheaper more rugged, and does not require a cold-chain. In the area of drug delivery, I will highlight two technologies: (1) an injectable delivery system based on thermally sensitive polypeptides for the sustained and tunable release of peptide drugs from a subcutaneous injection site that we have developed for treatment of type 2 diabetes; and (2) attachment-triggered self-assembly of recombinant peptide polymers that packages small molecules into soluble polymer nanoparticles that can improve the efficacy of many cancer chemotherapeutics.

Dr. Gopal C. Kundu Scientist 'G', National Centre for Cell Science, NCCS Complex, Pune 411 007, Maharashtra Email: [email protected]

Dr. Gopal Kundu has obtained his Ph.D. in chemistry from Bose Institute, Kolkata, India (1989). He did his post-doctoral research in US from 1989 to 1998. He joined as Scientist-D at National Centre for Cell Science (NCCS) in 1998 and at present he is Scientist-G. His area of research at NCCS is tumor biology, cancer stem cells, angiogenesis, cancer therapeutics and nanomedicine. He has received several awards including Fellows Award for Research Excellence from USA; National Bioscience Award; Shanti Swarup Bhatnagar Prize; International Award in Oncology, Greece; International Young Investigator Award, USA and 7th National Grassroots Innovation Award, Rashtrapati Bhavan, India. He is Fellow of National Academy of Sciences and Indian Academy of Sciences. He has published 84 papers including Nature Medicine, Science, PNAS, Cancer Research, J. of Invest. Dermatology, JBC, TCB, Oncogene, Nanomedicine etc and one US patent. He serves as Editorial Board Member of Current Molecular Medicine, Molecular Medicine Reports, American Journal of Cancer Research and Associate Editor of Molecular Cancer and Journal of Cancer Metastasis and Treatment. Dr. Kundu is also acting as Adjunct Visiting Professor at Curtin University, Perth, Australia.

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Nanoparticle-mediated Peptide-Conjugated Targeted Drug Delivery in Breast Cancer Amit S. Yadav and Gopal C. Kundu National Centre for Cell Science, NCCS Complex, Pune 411 007, India; E-mail: [email protected] Abstract: Breast cancer is a complex disease and most breast cancer treatments are limited to chemotherapy, radiation, and surgery. Substantial advances in breast cancer treatments have resulted in a significant decrease in mortality. However, existing breast cancer therapies often result in high toxicity and nonspecific side effects. Therefore, better targeted delivery and increased efficacy of drugs are crucial to overcome these effects. Application of nanotechnology or nanoparticle-mediated drug delivery can resolve some of these issues and these areas of research are expanding dramatically. In this study, cRGD peptide conjugated chitosan nanoparticles loaded with anticancer drug raloxifene or andrographolide or in combination of both are being synthesized and characterized with well-defined properties. Enhanced uptake of Cy5.5 conjugated RGD CHNP was studied in triple negative and αvβ3 integrin over-expressing breast cancer cells. The decrease in cell viability was observed by MTT assay after treatment with drug encapsulated nanoparticles and RGD conjugated nanoparticles exhibit enhanced inhibition of cell viability in these breast cancer cells. Finally, andrographolide or raloxifene encapsulated RGD-CHNPs significantly inhibited the breast tumor growth suggesting that RGD conjugated chitosan nanoparticles could be an effective approach for targeted therapeutic delivery in different sub-types of breast cancer.

Prof. Veena Koul Professor Indian Institute of Technology Delhi Hauz Khas,New Delhi-110016 veenak[at]cbme.iitd.ac.in Dr. Veena Koul received her PhD from Kashmir University in Medicinal Chemistry In the year 1986 she joined as a Senior Scientific officer in Centre for Biomedical Engineering at IIT Delhi and contributed significantly in high impact Projects like Clinical trials on Male Injectable Contraceptive, Equipment for Mass Health Care, UNFDP funded project on National Centre for Evaluation of Female Contraceptives. In the year, 1997, she joined as an assistant professor in the Centre. Her research interests are mainly focused in the area of drug delivery using polymeric systems for biomedical applications and medical devices. She has over 120 international publications and conference papers. EXPERTISE: Biomaterials, Medical Devices, Clinical Diagnostics, Drug Delivery Systems, Medical Chemistry and Polymer Chemistry.

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Redox Sensitive Nano platforms for Drug & Gene delivery Veena Koul Centre for Biomedical Engineering,Indian Institute of Technology, New Delhi & AIIMS, New Delhi Abstract: Redox sensitive nanosystems are smart nanocarriers which can trigger the release of loaded cargos in response to redox conditions. Tumor cells overexpress glutathione (GSH), a tripeptide which modulates the cellular redox microenvironment, in comparison to normal healthy cells. GSH levels in cancer cells range from 2-10 mM, which is 100-1000 times higher than that of blood and extracellular fluids and ˜ 3-4 fold higher than that of normal cells. Incorporation of redox sensitive linkages in the nanosystems shall facilitate their immediate release of loaded active agents within the redox microenvironment of cancer cells. In case of nanocarriers designed for gene delivery, the sheddable hydrophilic shell is generally composed of cationic structures which could electrostatically bind and condense the nucleic acids. Redox sensitive nanoplatforms can also be designed for co-delivery of drugs and genes in which the inner core entrapping the drug molecules would comprise of hydrophobic parts to which cationic groups containing hydrophilic structures are linked via disulfide linkages. Co-delivery of drugs and nucleic acids is gaining importance in the treatment of various diseases like cancer due to its ability to overcome both pump related and non-pump related drug resistances. Nanocarriers can overcome the efflux pump related drug resistance by following endocytotic pathway during their cellular uptake and simultaneous delivery of nucleic acids, like siRNA, miRNA, shRNA etc., can knock down the non-pump mediated anti apoptotic defense mechanism of cancer cells. The major challenge in accomplishing this therapeutic strategy is the designing of an efficient nanocarrier which could load and co-deliver both the therapeutic agents simultaneously and specifically to cancer cells. The talk will present how redox sensitive polymeric micelles based on alternating co-polymers like poly (styrene –alt-maleic anhydride) have been developed by our own research group and depicted excellent co-delivery efficacy of anticancer drug and nucleic acid, in vitro and in vivo. However, only a limited number of these nanosystmes have been taken for clinical trials. These redox sensitive nanosystems need to be properly investigated in clinical settings, which in turn would help in developing clinically efficient therapeutic nanoformulations.

Lakshmi S. Nair Associate Professor, Department of Orthopedic Surgery, UConn Health CenterPh.D., SCTIMST (1999) University of Connecticut Health Center 263 Farmington Avenue, Unit MC 3711, Farmington, CT 06030 Email: [email protected] Background • Postdoctoral Fellow, Department of Chemical Engineering, Drexel University, 2001-2003 • Scientist-B, Polymer Chemistry, SCTIMST, India, 2000-2001 Expertise • Biomaterial Design and Synthesis, Injectable Hydrogels • Protein and Small Molecule Delivery • Nanoparticles, Cell Instructive Polymer Matrices to Direct Regeneration • Platelet Rich Plasma Delivery • Gene Activated Matrices to Enhance Tissue Regeneration • Biomaterial based Strategies for Pain Management

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Injectable hydrogel systems with tunable properties for biomedical applications Lakshmi S. Nair Department of Orthopaedic Surgery, Department of Biomedical Engineering, Department of Material Science and Engineering, Institute for Regenerative Engineering, University of Connecticut, USA Abstract: Injectable hydrogels are attractive alternatives to implantable delivery systems as they can provide a cell friendly mild environment and allow for minimally invasive administration via endoscopic and percutaneous procedures. We have developed injectable hydrogels as well as composite systems as localized delivery vehicle via mild enzyme mediated/chemical crosslinking of biocompatible polymers. We have investigated the feasibility to modulate the physical, mechanical and biological properties of these injectable hydrogels by varying its chemistry and crosslinking methods. Our studies on the localized delivery of cells, proteins and small molecules using injectable hydrogels/composites for various biomedical applications will be discussed.

Neetu Singh Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, 110016, India. All India Institute of Medical Sciences, Ansari Nagar, New Delhi,110029, India. E-mail: [email protected] Dr. Neetu Singh did her MSc from University of Mumbai, India, Phd from Georgia Institute of Technology, Atlanta, GA, USA, she has been a post-doctoral associate in Massachusetts Institute of Technology, Cambridge, MA, USA. She is a recipient of the “Innovative Young Biotechnologist award 2013” by DBT, India. Neetu Singh’s group is interested in integrating concepts and skills from chemistry, materials science, and biology to design nano-structured materials with enhanced functionality for applications in biomedical implants, cancer diagnostics, and drug delivery. Her group particularly focused on seeking a systematic understanding of the bio-activity of nanostructures and developing specific "structure-bioactivity" relationships. She believes that such a rational approach is necessary for systematically advancing nano-materials and biomedical technologies of the future. Projects in her lab can be broadly categorized into the following sub-areas: (1) Structure-bioactivity relationships in design of biologically relevant nanostructures. (2) Development of new functional nanomaterials. Carbon Dots for Drug Delivery and Cellular Microenvironment Sensing Neetu Singh Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, 110016, India. All India Institute of Medical Sciences, Ansari Nagar, New Delhi,110029, India. E-mail: [email protected]; Tel: + 91-11-26591422 Abstract: The growing need for new, functional, biologically active materials has arisen from our inability to detect and treat many diseases with traditional therapies or procedures. Nanomaterials are showing great potential in the fields of biomedicine and biology not only due to their unique size-dependent properties but also since their size is on a similar scale as that of proteins and other biomolecules. This presentation will focus on strategies for making carbon 27

dots customized to have desirable properties for various bio-applications in drug delivery and bio-imaging. For instance, despite the promising photo-physical properties of fluorescent graphene quantum dots (GQDs), their cellular toxicity needs to be addressed before their full potential could be completely realized in biomedicine. A simple method for mitigating the toxicity of GQDs by embedding them in PEG matrix will be discussed. These more biocompatible p-GQDs have enhanced loading and efficient intracellular delivery of therapeutics. In yet another example, the talk will highlight the development of a fluorescence carbon dot based platform, which can detect changes in pH in the cell microenvironment. pH values in living system plays important role in deciding rate of chemical reactions, health of organ, tissue or body. Variation in pH can be used to detect and monitor a disease. Organic pH sensitive fluorescent dyes have been developed which show pH sensitive change in fluorescence output. These dyes suffer from problems of photo bleaching or toxicity or both. The developed carbon dot based pH nanosensor can be encapsulated with live cells in transparent spherical PEG microgel using droplet based microfluidics and UV photo-polymerization. The fluorescent carbon dots have excitation dependent emission, which was found sensitive to changes in pH in the range of 10 to 4. The intensity of emission increases as the pH is lowered and becomes more acidic. Since, the nanosensors can sense pH changes in the physiologically relevant range of 7.6 to 5.8, the developed system can be used to study biological process or disease progression in 3D. As an example of the application of these pH nanosensors, development of a platform for sensing bacterial growth will be discussed.The platform can differentiate between resistant and non-resistant strains of E.coli in ~ 6 hours, which is better than currently used clinical methods.

Gabriele Candiani Associate Professor, Department of Chemistry, Polytechnico of Milano, Italy Email: [email protected] Background: • Ph.D. (Biomedical Science) • M.Sc. (Biological Science) Expertise: • Micro and nanoparticles for drug and gene delivery • Tissue Engineering • Antibacterial materials for indoor air treatment systems Non-viral gene delivery vectors: what is known and what is not. Gabriele Candiani Department of Chemistry, Materials and Chemical Engineering “Giulio Natta”, Politecnico di Milano, Milan, 20131, Italy. Email: [email protected] Abstract: Gene delivery is the transfer of genetic material into recipient cells to alter some functions. As the spontaneous entry of intact nucleic acids into cells is unfortunately very inefficient, cationic lipids and polymers, collectively known as non-viral delivery systems, have made their breakthrough in basic and medical research. Such molecules do self-assemble with polyanionic nucleic acids to give rise to nano- and micro-particles that are taken up by cells to elicit their function. Since non-viral vectors currently available do unfortunately suffer from low 28

transfection efficiency and remarkable toxicity, there is an urgent need of more effective vehicles and new tools for the straightforward and quantitative assessment of transfection efficiency and cytotoxicity at once. This talk will chronicle the road towards the development of more and more effective gene delivery vectors. In this regard, major strides forward have been recently made in the development of stimuli-responsive gene delivery vectors that actively respond to changes in the (micro)environment (e.g., cell enzymes, redox status and pH) by altering their properties and behavior. Besides, easy-to-use lab-on-chip platforms to perform transfection assays for unbiased, high-throughput selection of more and more effective gene delivery vectors will be presented as well. Selected references:

 Maiolo D*, Colombo J, Beretta J, Malloggi C, Candiani G*, Baldelli Bombelli F. The polyplex, protein corona, cell interplay: Tips and drawbacks. Colloids Surf B Biointerfaces. (2018). DOI: 10.1016/j.colsurfb.2018.01.040  Giupponi E, Visone R, Occhetta P, Colombo F, Rasponi M, Candiani G*. Development of a microfluidic platform for high-throughput screening of non-viral gene delivery vectors. Biotechnol Bioeng. (2018). DOI: 10.1002/bit.26506

Dr. Akash Gulyani Assistant Professor - Institute for Stem Cell Biology and Regenerative Medicine, National Center for Biological Sciences Email: [email protected]

Background B.Sc Hons: Chemistry (BHU, Varanasi, India); M Sc: Biotechnology (M.S University, Baroda, India); Ph.D: Biochemistry and Metabolic Engineering (IIT, Mumbai, India) Research Interest Protein engineering and directed evolution to build highly specific and sensitive fluorescence based biosensors for critical signalling proteins that are misregulated in disease states. Fluorescent biosensors for cellular dynamics and discoveries in natural light sensing and eye-brain regeneration Akash Gulyani Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, India Abstract: I will present our multi-faceted approach to sensing dynamics across scales, with emphasis on building new sensing methods and tools. Cell behavior is controlled by highly dynamic signaling networks wherein the activities of key signaling proteins are tightly regulated inside the cell. I will present the development of fluorescent biosensors based on engineered protein binders that recognize 'active' signaling proteins or conformations in live cells. I will describe how a new biosensor for a specific Src family kinase, Fyn shows how signaling systems are tightly compartmentalized inside the cell. Biosensor imaging also reveals that Fyn activity is spatially-temporally regulated and is subject to cross-talk between cell adhesion receptors and growth factor receptors (receptor tyrosine kinases RTKs). Additionally, I will also present the development of novel mitochondrial probes and dyes help visualize unexplored patterns in mitochondrial activity and organization in live cells. 29

I will end my talk by showcasing our recent work where we have discovered new kinds of light sensing in nature. Our work reveals how organisms possessing simple eyes and brain can accomplish sophisticated gradient and spectral sensing, while also performing complex neural processing. I will also show how several planarian flatworm species have highly acute and sophisticated light sensing independent of the eye. These findings not only impact on our understanding of how light is sensed in nature but also allow us to study eye-brain regeneration using simple organisms in unprecedented ways.

Dr. Vijayalakshmi Venkatesan Scientist ‘F’ & HoD, Stem Cell Biology National Institute of Nutrition Jamai-Osmania PO, Hyderabad E-mail: [email protected] Dr. Vijayalakshmi carried out her post doctoral research at the Centre for Cellular and Molecular Biology (CCMB), Hyderabad and worked in the research area of Cell biology towards “Understanding the Cellular and Molecular Regulation of the Genomic and Non-genomic responses of Estradiol and modulation of the Transglutaminase activity in the Vaginal epithelial Cells”. Subsequently, joined as Faculty, Scientist ‘D’ (DBT-Supported) at Centre for Liver Research, Owaisi Hospital & Research Centre, Hyderabad and worked in the area of Hepatobiology and cellular therapy in the management of acute liver failure. Joined NIN in 2002, and currently heading the stem cell facility at NIN. The primary focus has been to harness the potential(s) of stem cells isolated from the adult tissue /progenitors and we are trying to understand the nutrient interactions in the microenvironment of the stem cells and their programming towards the insulin secreting cells. This is essentially from the primary cultures islets, intra islet precursors and mesenchymal cells. This also involves the Tissue culture work and using the state-of-th-art techniques such as Confocal microscopy, FAX and PCR the cellular and molecular characterization is being undertaken. Her Division is also developing the cell lines from the adipose, pancreatic and Kidney tissues of the NIN mutant Obese rats funded by DBTwith NIN-NCCS as an alternate to the in vivo system. The efficacy of Human Umbilical Cord Serum(HUCS) as an alternative to FCS is also being investigated in primary cultures,stem cells and cell lines.

Mesenchymal Stem Cells to Avert Preclinical Diabetic state - Our Experience(s) from Obese Mutant rat model system : Vijayalakshmi Venkatesan Stem Cell Lab and Biochemistry, National Institute of Nutrition, Hyderabad, India.

Abstract: Obesity, Type 2 diabetes and CVDs constitute a deadly metabolic triad of noncommunicable diseases (NCDs), and form the major public health concerns across the globe more so in Asian Countries like India. The etiology is multifactorial with inflammation viewed as the predominant pathology underlining NCDs. The impetus obtained from our earlier studies using obese Mutant rat model (Muts) demonstrated metabolic dysfunctions,altered cross talk 30

between systemic to progenitor pool,and with significant inflammatory milieu in adipose tissue and pancreas , exacerbating with age ( 6-9 month>1-5 months) . Addressing these events in Muts, we have in the present study explored for the beneficial effects of adult stem cells i.e Mesenchymal stem cells (MSCs) ,which are endowed with inherent immunomodulatory, anti inflammatory functions and to initiate repair process at the site of injury owing to its autocrine and paracrine secretions. Thus,Intramuscular injection of hPMSCs (1x106) to Muts showed significant beneficial effects to overcome the metabolic insult in Muts.These include: reduction in Insulin Resistance (HOMA-IR), Impaired Glucose Tolerance, reduced cellular stress and inflammation and improved insulin secretory functions. Corroborating with our other findings, in vivo tracking of the fluorescent / DiD labelled hPMSCs in Muts showed increased homing at the region of the visceral adipose and seems to have negated the inflammatory milieu vis a vis enhanced immunomodulation. Our findings advocate for the possible clinical applications of MSCs in the management of preclinical diabetes either to prevent or delay the metabolic alterations leading to frank diabetes.

Dr. Thomas J Webster Chair and Professor of Chemical Engineering Northeastern University, Boston, MA 02115 Email: [email protected] Professor Webster directs the Nanomedicine Laboratory which designs, synthesizes, and evaluates nanomaterials for various implant applications. Dr. Thomas J. Webster’s degrees are in chemical engineering from the University of Pittsburgh (B.S., 1995) and in biomedical engineering from Rensselaer Polytechnic Institute (M.S., 1997; Ph.D., 2000). He is currently the Department Chair and Professor of Chemical Engineering at Northeastern University in Boston. His research explores the use of nanotechnology in numerous applications. Specifically, his research addresses the design, synthesis, and evaluation of nanophase materials (that is, materials with fundamental length scales less than 100 nm) as more effective biomedical devices. He has completed extensive studies on the use of nanophase materials to regenerate tissues and has graduated/supervised over 109 visiting faculty, clinical fellows, post-doctoral students, and thesis completing B.S., M.S., and Ph.D. students. To date, his lab group has generated over 9 textbooks, 48 book chapters, 306 invited presentations, at least 403 peer-reviewed literature articles, at least 567 conference presentations, and 32 provisional or full patents. His H index is 47. Some of these patents led to the formation of 9 companies.

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Design, fabricating, and commercializing in-the-body nano sensors: The future of health Thomas J. Webster Art Zafiropoulo Chair, Department of Chemical Engineering, Northeastern University, Boston, MA, 02115, USA; Editor, International Journal of Nanomedicine; Associate Editor, Nanomedicine: NBM; Past President, US Society For Biomaterials; Fellow, AANM, AIMBE, BMES, FSBE, and NAI Abstract: Synthetic materials used in medical devices today are typically composed of micron sized particles, grains, and/or fiber dimensions. Although human cells are on the micron scale, their individual components, e.g. proteins, are composed of nanometer features. By modifying only the nanofeatures on material surfaces without changing surface chemistry, it is possible to increase tissue growth of any human tissue by controlling the endogenous adsorption of adhesive proteins onto the material surface. In addition, our group has shown that these same nanofeatures and nano-modifications can reduce bacterial growth without using antibiotics, which may further accelerate the growth of antibiotic resistant microbes. Inflammation can also be decreased through the use of nanomaterials. Nanomedicine has been shown to stimulate the growth and differentiation of stem cells, which may someday be used to treat incurable disorders, such as neural damage. However, in moving beyond tissue engineering and medical devices, it is clear that for many diseases, we need real time monitoring of body health. In this manner, some of the same materials utilized above are being used to develop implantable sensors that can both monitor and heal diseased cells. This invited talk will highlight some of these advancements, particularly those approved by the FDA

Prof. K. Indira Priyadarsini Head, Radiation Chemistry & Biology Section [email protected] Development of Health Care Products: Nutraceuticals and radioprotectors K. Indira Priyadarsini Chemistry Division, Bhabha Atomic Research Centre, Mumbai-400085, India E-mail: [email protected] Abstract: There is a significant raise in lifestyle diseases mainly due to changes in living habits, dietary issues, industrialisation and pollution. This has led to growing demand of health care products as preventive measures, especially from dietary sources. Nutraceuticals, “food or parts of food that provide medical or health benefits, including the prevention and treatment of diseas”, are gaining significance world wide as nutritional therapeutics especially for life style diseases. In the past, our group did extensive research on turmeric, a spice used regularly by Indians, and its active ingredient curcumin, a polyphenol. As a spin off from this research we have developed a nutraceutical, a dietary protein supplement providing daily necessary supplementation of curcumin. Similarly several nano-bioavaialble curcumin formulations have been prepared which find applications in development of curcumin enriched functional foods. Two such technologies were transferred to the Indian industry not only to provide value addition to turmeric but also to use our country’s biodiversity. 32

Another important research programme in health care products is development of novel selenium compounds as radiotherapy adjuvants. Selenium is a micronutrient for humans and plays a role cellular homeostasis through regulating redox enzymes. In this direction several aliphatic water soluble selenium compounds were synthesised and screened for radioprotection in cell lines. Based on these results, one compound, diselenodipropionic acid (DSePA,) was identified. DSePA showed potential radioprotection ability under in vivo models, especially as an adjuvant for preventing pneumonitis, a serious side effect during thoracic radiotherapy. Some of these findings will be discussed in the lecture.

Prasad KDV Yarlagadda Queensland University of Technology, Australia Email - [email protected] Prof. Prasad Yarlagadda worked in industry and university over 30 years. He is currently Professor in Smart Systems and also member of Injury Prevention and Trauma Management group in Queensland University of Technology, Australia. He had number of distinguished appointments in various universities in India, China and Australia. He received more than $15M funding for his research and he published 500 papers. In 2016 Professor Yarlagadda was awarded Order of Australia Medal and included in Queens Birthday Honors list in recognition of his outstanding service to Engineering Profession, Australia. He is Editor in Chief and Deputy Editor-in-chief of 4 international journals, and also editorial board member of 18 ISI listed Journals. IN addition he is also held number of leadership roles in Professional societies as President, Society of Manufacturing Engineers and Fellow of many professional organizations such as Engineers Australia, World Academy of Manufacturing and Materials ASME, SME.

Studies on deformation of pre-contoured fracture fixation plates for their applications in orthopaedic surgeries Prasad KDV Yarlagadda School of Chemistry, Physics and Mechanical Engineering Science and Engineering Faculty Queensland University of Technology 2, George Stret, Brisbane Qld 4001, Australia Abstract: The ultimate objective of the bone fracture treatment is to stabilize the fracture and restore the function of the limb. Anatomically pre-contoured fracture fixation plates are a treatment option for bone fractures. A well-fitting plate can be used as a tool for anatomical reduction of the fractured bone. However, recent studies showed that some plates fit poorly for many patients due to considerable shape variations between bones of the same anatomical site. Therefore, the plates have to be manually fitted and deformed by surgeons to fit each patient optimally. The process is time-intensive and labour-intensive, and could lead to adverse clinical implications such as wound infection or plate failure. This talk addresses a new iterative method to simulate the patient-specific deformation of an optimally fitting plate for pre-operative planning purposes and an innovative automatic machine development which facilitates the deformation of the bone fracture fixation plates. . It further demonstrates the validation of the method through a case study. The proposed method involves 33

the integration of four commercially available software tools, Matlab, Rapidform2006, SolidWorks, and ANSYS, each performing specific tasks to obtain a plate shape that fits optimally for an individual tibia and is mechanically safe. A typical challenge when crossing multiple platforms is to ensure correct data transfer. We present an example of the implementation of the proposed method to demonstrate successful data transfer between the four platforms and the feasibility of the method.

Dr Manoj Gupta Associate Professor Department of Mechanical Engineering, National University of Singapore, Singapore 117 576 Email: [email protected] Background  PhD-University of California at Irvine, USA, MEng, IISc, Bangalore, India (Gold Medallist)  BEng, VRCE, Nagpur, India   

Expertise Development of Energy Efficient Techniques such as Microwave Processing and Disintegrated Melt Deposition Processing. Development of light-weight materials with current focus on magnesium based nanocomposites. Development of lead free solders with good strength and exceptional ductility.

An Insight into Magnesium Based Materials for Biomedical Applications M. Gupta Department of Mechanical Engineering, National University of Singapore, Singapore 117 576 Email: [email protected] Abstract: Magnesium is an essential element required by human body for performing multiple biological functions. Humans need about 25-400 mg of magnesium every day which is second only to calcium. The intrinsic requirement of magnesium by human body has triggered widespread interest in research community to utilize it as a non-permanent implant. Most targeted applications are envisioned in orthopedic domain while investigations are also ongoing to use it as stents and surgical clips. As an example, use of titanium clips lead to post-operative complications and problems associated with diagnostic imaging which can be circumvented using dissolvable magnesium clips. In view of the potential benefits of magnesium based materials in biomedical sector, this talk will focus in providing an insight into emerging magnesium based materials which can be potential biomaterials in very near future. Keywords: Magnesium; composites, alloys, implants

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Dr. S. C. Kundu ERA Chair Full Professor at 3Bs Research Group, Headquarters of the European Institute of Tissue Engineering and Regenerative Medicine, University of Minho, Portugal. Email: [email protected] Dr. SC Kundu received his under graduate degree (B.Sc Hons.) in Agricultural Sciences from Visva Bharati University, Santineketan, India, master degree (M. Sc.) in Genetics and Plant Breeding and PhD in genetics from Banaras Hindu University, Varanasi, India. Dr. Kundu received his post-doctoral training at Institute of Molecular Biology, Moscow; Department of Biology, York University, Canada; Medical University, Lubeck, Germany; Department of Biology & Biochemistry, Brunel University, UK. Research Area:  Silk biomaterial matrices  3D cancer models  Cell culture  Drug screening  Bioactive molecule delivery Awards and honors: • Distinguished Invited Professor at Dankook University, South Korea. • Published 160 major research articles in journals such as Chromosoma, Experimental Cell Research, Cytogenetics and Cell Genetics, Euro J Cell Biology, Methods in Enzymology, J Biological Chemistry, Biotechnology and Bioengineering, Acta Biomaterialia, Biomedical Microdevices, Biomaterials, Macromolecular Bioscience, Progress in Polymer Science, Biotech Advances, Soft Matter, Tissue Engineering A, Nanoscale, Advanced Functional Materials, Advanced Materials and others. • Editorial board member of Biomaterials, Scientific Reports, Journal of Regenerative Medicine & Tissue Engineering, Journal of Biological Engineering, Journal of Biomedical Materials. • Fellow of the National Academy of Sciences, India, West Bengal Academy of Sciences, India and Alexander von Humboldt, Germany. Engineering 3D approaches to model cancer metastasis Banani Kundu1,2, Virginia Brancato1,2, David Caballero1,2, Joaquim M Oliveira1,2,3, Vitor M Correlo1,2,3, Rui L Reis1,2,3 and Subhas C Kundu1,2 1

3B’s Research Group, I3Bs – Biomaterials, Biodegradable and Biomimetics,University of Minho; 2ICVS/3B’s – PT Government Associate Laboratory, Braga/Guimarães 4805-017, Portugal; 3The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, AvePark, 4805-017 Barco, Guimarães, Portugal. Background: Engineered 3D approaches offer lucid recapitulation of physiological cancerous micro-niche in vitro using advanced biomaterials and culture techniques. Current breakthrough integrates the approaches of tissue engineering (biomaterials, cells and signaling molecules) and microfluidic technologies to provide control over precision.

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Methods: 3D tumor organoids were generated using proteins (collagen, silk) or polysaccharide (gellan gum) in static (hydrogels, scaffolds) and dynamic (microfluidic) environments. The micro-environments contributed to the spatial, rheological and mechanical stimuli to cells. The state-of-the-art bioengineering approaches included human cancer cells (breast, bone, and lung), endothelial cells, cancer associated fibroblasts and stem cells – in order to mimic the multi-cellular content of the metastatic niche. Tumor organoids’ behavior was monitored using biochemical assays (alamar blue, live-dead, and pico-green assay), imaging techniques (phase-contrast, confocal, scanning electron microscopy), gene analysis, histology and immuno-histochemistry. Results: Using 3D silk-based breast cancer model, both non-invasive and metastatic breast cancer cells and normal mammary fibroblasts were co-seeded resulting in an altered expression of collagen I, MMP-2, MMP-3 and MMP-9. This reveals the detail insight mechanisms of organogenesis. The biochemical and mechanical dynamics of multi-cellular cancer stroma were recapitulated using blends of silk and gellan gum to elucidate extracellular matrix based therapeutic targets, which drive the formation of cancer spheroids. Using a microfluidic device, metastatic lung organoids embedded within a 3D collagen matrix developed continuous and elongated invading protrusions towards the channel mimicking human blood vessel. The injection of a pharmacological inhibitor of the Rhopathway through the blood vessel-like channel resulted in a perturbation of the protrusion dynamics. Conclusion: 3D bioengineered models show the applicability to assess the mechanisms that govern cancer cell invasion and early stage screening of potential cancer therapeutics. Acknowledgement: European Union Framework Programme for Research and Innovation Horizon 2020 under grant agreement nº 668983 – FoReCaST Keywords: Cancer, metastasis, biomaterials, microfluidics, silk

IVD degeneration and its consequences - an injectable tissue-engineered therapy Annie John Advanced Centre for Tissue Engineering, Dept. of Biochemistry, University of Kerala, Kariavattom, Thiruvananthapuram - 695 581, India. Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Thiruvananthapuram - 695012, India. E mail: [email protected] Abstract: The skeleton is a dynamic system that confronts posture changes in life. The intervertebral disc (IVD) of the skeleton comprises of the nucleus pulposus (NP) and Annulus Fibrosis (AF) which act as cushions between the vertebrae of the vertebral column. IVD degeneration gradually happens in early life due to unusual sitting, trauma, genetic inheritance and impaired nutrient exchange between NP and blood vessels. The NP is the first tissue to initiate degeneration and subsequently the IVD height will collapse, probably leading to low back pain which has emerged as the most expensive global healthcare problem. Treatment modalities involve conservative management/surgical intervention/NP replacement. Over the last decade, there has been a surge of interest in applying tissue-engineering principles (scaffold and cells) to treat problems associated with IVD. Alginate Strontium (Alg/Sr) hydrogel is a promising scaffold which can encapsulate cells for secreting extracellular matrix. Alg/Sr 36

hydrogel was tested for its strength, safety in vitro and further by mimicking an in vivo 3D structure incorporating cells. This ‘combination product’ was ultimately injected in situ at the damaged NP site of the lumbar vertebrae in New Zealand White Rabbits to restore the collapsed disc height. The aim of an injectable tissue-engineered therapy is to repair the degeneration of the disc at the cellular level and increase the ECM - a relief for low back pain commonly occurring during the second or third decade of life.

Dr. R. Selvakumar Associate Professor in Nanobiotechnology PSG Institute of Advanced Studies, Coimbatore-641004, Tamil Nadu, India E-Mail: [email protected]

Dr.R.Selvakumar completed his Ph.D in Biotechnology from Bharathiar University India and currently working as Associate Professor in Nanobiotechnology, at PSG Institute of Advanced Studies, Coimbatore, India. His area of interest are Bone and cartilage Tissue Engineering, Biomaterials-biomolecule interaction for Tissue Engineering application. He is a recipient of several international fellowships like, Endeavour research fellowship, Australia, WARI fellowship, USA and Blaustein fellowship, Israel and national fellowships like CSIR-SRF from Govt. of India. He has more 56 publications that have been cited over 523 times. His H- index is 13. He has filed three patents and has transferred his technology on Design, fabrication & method of producing micro, meso and nanoporous three dimensional (3D) scaffolds for Tissue Engineering using removable needle technology, to Intellectual Ventures, USA. He is a active reviewer in various journals. Three dimensional porous, biomolecule supplemented Silk-PVA composite scaffold reinforced with natural eggshell membrane for human knee meniscal tissue engineering Mamatha M. Pillai1, J. Gopinathan2, R. Senthil Kumar2, G. Sathish Kumar2, S. Shanthakumari3, K. Santosh Sahanand4, Amitava Bhattacharyya2*, R. Selvakumar1* 1 2

Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore-641004, India Nanoscience and technology laboratory, Dept of Electronics and Communication Engineering, PSG College of Technology, Coimbatore-641004, India 3 Department of Pathology, PSG Institute of Medical Sciences and Research, Coimbatore641004, India 4 Ortho One Orthopaedic Speciality Centre, Coimbatore-641005, India *Correspondence to: Dr. R Selvakumar, email: [email protected], Dr. Amitava Bhattacharyya, email: [email protected]

Abstract: Natural biomaterial silk fibroin (SF) and a synthetic polymer poly vinyl alcohol (PVA) along with autoclaved egg shell membrane (AESM) based 3D porous scaffolds were developed and analyzed in vitro and in vivo to study its potential application in human knee meniscus tissue engineering. 3:1 SF-PVA scaffolds were reinforced with AESM powder using different concentrations (1-3%) to enhance the biomechanical properties of scaffolds. Further to improve cell attachment and proliferation these 3D porous scaffolds were functionalized using a unique combination of biomolecules (UCM- biotin-20 µg/ml, glucose-60 µg/ml, CS-60 µg/ml and proline-20 µg/ml). A comprehensive analysis of developed 3D scaffolds was carried out on structural, mechanical and biological functionalities including FESEM, porosity, functional 37

group analysis, swelling ratio, mechanical characterization, in vitro degradation, and in vitro meniscus tissue engineering. To analyze the biocompatibility scaffolds were subcutaneously implanted in New Zealand white rabbits and immunohistochemical analysis was performed. The results showed that mechanical properties of AESM added scaffolds enhanced as the concentration increases. Cytotoxicity and SEM analysis indicated that the developed 3D porous scaffolds were nontoxic and exhibited better cell proliferation in SF-PVA- AESM 3% scaffold. In vivo results indicate that SF-PVA scaffold reinforced with 3%AESM is a promising scaffold for meniscus tissue engineering application. Keywords: SF-PVA composites, 3D porous scaffold, meniscus tissue engineering, autoclaved egg shell membrane, reinforcement, functionalization In vitro biocompatibility study of additive manufactured titanium cellular structures for mandibular implants 1* M. Jaivignesh , A. Suresh Babu2, G. Arumaikkannu3 1 Research Scholar, 2Assistant Professor (S.G), 3Professor Department of Manufacturing Engineering, College of Engineering Guindy, Anna University, Chennai - 600 025, Tamil Nadu, India. BACKGROUND: Cellular structures assist in bone tissue formation since the pores allow migration and proliferation of live cells. The main objective of in-vitro biocompatibility test is to identify the physiological tolerance of the bone implants and the tissue growth around the foreign material. In this work, mandibular implant is considered for application of cellular structures and Ti64 being a biomaterial, is selected for study. METHODS: The Ti64 cellular structures were designed with 10mm x 10mm x 3mm size, pore size 1 mm and inter-pore distance 1.6 mm and were fabricated using direct metal laser sintering process. Nine samples were fabricated to study three samples each in three different time periods. The cellular response of was assessed in terms of cell viability and proliferation to the surface. MG-63 sarchoma cell were used for this study to mimic the bone cells associated with mandibular implant. Cells were grown in 75cm2 flask containing Dulbecco’s modified Eagle’s medium (DMEM; Sigma) the mediums were supplemented with 10% Fetal bovine Serum (FBS; invitrogen), 1.5 g/l sodium bicarbonate, 10,000 Units/ml penicillin, 10 mg/ml streptomycin and 25μg/ml Ampotericin B. The viability of cells was assessed by standard MTT (3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. This assay is based on the reduction of soluble yellow tetrazolium salt to insoluble purple formazan crystals by metabolically active cells. Only live cells are able to take up the tetrazolium salt. In-vitro analysis of cellular structure was carried out by measuring the viable cells after a particular time interval of 12th hour, 24th hour and 36th hour. RESULTS: On an average, the control samples showed 100 % viability whereas treatment samples showed 139.8 % viability i.e there is an improvement in cell growth with respect to Ti64 cellular structures. CONCLUSION: Thus the direct metal laser sintered Ti64 cellular structure is suitable for mandibular implant application. KEYWORDS: Biocompatibility, Cellular Structures, Direct Metal Laser Sintering, Mandibular Implant, Ti64

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Jean Jacques Pireaux Department of Physics, Research Group on Carbon Nanostructures (CARBONNAGe), University of Namur, Belgium Email: [email protected] Background: • Doctor of Science (with thesis) Expertise: • Development of catalytic surfaces working at room temperature for the depollution of domestic air • Long life materials for hydrogen-air PEM fuel cells • Versatile Catalyst Materials Fabrication by Low-Pressure Plasma Treatments Active coatings for cardiovascular stent applications: from materials and surface science to biological performances V. Montano-Machado1, P. Chevallier1, S. Turgeon1 and D. Mantovani1 C. Noël2, L. Houssiau2 and Jean-Jacques Pireaux2 1 : Laboratory for Biomaterials and Bioengineering, Laval University, Québec, Québec G1V 0A6, Canada 2 : Namur Institute of Structured Matter (NISM-LISE), University of Namur, rue de Bruxelles 61, B-5000 NAMUR, Belgium [email protected]

Abstract: Metallic stents for cardiovascular applications can be coated to improve their passive biocompatibility in the blood circulatory system, and prevent restenosis ; this can be obtained with PTFE-like fluorocarbon or TiO2-like coatings … that are expected not to trigger a bioresponse. Active bio-coatings are those targeting directly a bio-response, like e.g. a fibronectin (FN) coating to promote endothelialization, or a phosphorylcholine (PRC) coating to attain hemocompatibility. In a materials scientist’s approach, we ‘ll briefly review (1) the different physical and chemical methods –like vacuum plasma, solution coating, chemical grafting- used to deposit a thin and adherent film of a given nature, and (2) the characterization spectroscopies used to ascertain the physico-chemical properties (elemental, chemical and molecular composition, structure) of the deposited layers ; X-ray Photoelectron Spectroscopy(XPS), contact angle (CA) and Time-of Flight Secondary Ion Mass Spetrometry (ToF-SIMS) will deserve particular attention. As demonstration example, the case of a coating containing both FN and PRC molecules, will be detailed. Prepared by four different methods, the bi-layers are first characterized, and then evaluated for their biological performances, using haemolysis rate measurement and endothelial cell viability test. Grafted FN exhibits denser coatings, while adsorbed PRC presents a better homogeneity ; these are probably the reasons why the layer preseting a combination of grated fibronectin and adsorbed phosphorylcholine exhibits the best biological endothelialization and hemocompatibility properties.

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Roberto Fanganiello Universidad Nacional Autónoma of México, Mexico Email – [email protected]

Roberto D. Fanganiello holds a Bachelor of Science (BSc) in Biology from the University of Sao Paulo, with emphasis in molecular and cellular biology, a Doctor of Philosophy (PhD) in Human Molecular Genetics from the Department of Genetics and Evolution at the University of Sao Paulo and the Department of Orthopaedics and Rehabilitation at the Yale University School of Medicine, and a post-doctoral fellowship in Tissue Bioengineering and Stem Cell-based Regenerative Medicine from the Institute of Biosciences and the Human Genome and Stem Cell Research Center at the University of Sao Paulo. Expertise- molecular genetics, biochemical pathways and cellular events underpinning human craniofacial dysmorphologies, genetic counselling for patients with craniofacial syndromes, musculoskeletal/bone tissue bioengineering based on adult stem cells in association with biomimetic scaffolds and molecular dissection of stem cell differentiation signalling pathways Honors and awards Roberto is an active member of the American Society for Human Genetics (ASHG), International Society for Cellular Therapy (ISCT, as an industrial partner as well as part of the Commercialization Committee and Strategies for Commercialization Track subcommittee), and the International Anaplastology Association (IAA, as part of the board of directors). Transcriptional and molecular events underlying heterogeneity in the osteopotential of human mesenchymal stromal cells Roberto Fanganiello Universidad Nacional Autónoma of México, Mexico Abstract: Accurate understanding of the molecular mechanisms underpinning mesenchymal stem cells (MSCs) differentiation potential is pivotal to accomplishing a solid translation of MSCs-based experimental therapeutics to clinical practice. This knowledge also enables the use of exogenous factors and/or selection of MSCs` subpopulations to optimize this potential. We searched for markers predictive of osteopotential in two types of MSCs: stem cells from human deciduous teeth (SHED) and human adipose-derived stem cells (hASCs) and we report that IGF2 and CD105 expression levels are predictive of osteopotential in these MSCs. IGF2 is among the top upregulated transcripts before and during osteodifferentiation in SHED, with intrinsically higher osteopotential than hASCs, and exogenous activation/inhibition of IGF2 pathway lead respectively to higher and lower osteopotential in SHED and hASCs. We found IGF2 to be biallelically expressed in SHED, with a 4 % methylation increase in the imprinting control region within the IGF2-H19 locus. This is due to 2 specific CpG sites and we suggest that SHED IGF2 upregulation is attributable to loss of imprinting. CD105 expression is inversely correlated with osteopotential in these MSCs and we validated that CD105 can be used to select for subpopulations of SHED and hASCs with higher osteopotential. Last, we show that hsa-mir1287 regulates CD105 expression and propose that fine-tuning of hsa-mir-1287 levels could be used to control osteopotential in SHED and in hASCs. These findings help us to have a better discernment of the molecular basis of MSCs` osteogenic plasticity and open new perspectives to leverage osteogenic potential in MSCs. 40

Rodrigo S. Vieira Universidad Nacional Autónoma of México, Mexico Graduate at Chemical Engineering from Universidade Federal do Ceará (2001), master’s at Chemical Engineering from Universidade Estadual de Campinas (2004) and Phd at Chemical Engineering from Universidade Estadual de Campinas (2008). Sulfonated-modified chitosan for blood-interacting material Anaftália Morais(1); Clayton Campelo(2); Pascale Chevallier(2); Diego Mantovani(2); Rodrigo S. Vieira(1) 1. Chemical Engineering Department, Federal University of Ceará, FORTALEZA, Ceara, Brazil. 2. Faculté des sciences et de génie, Laval University, Quebec, QC, Canada. Abstract: Thrombosis constitute the main clinical problem when blood-interacting devices are implanted in the body. Coatings with thin polymer layers represent a recognized strategy to modulate interactions between the material surface and the blood environment. To provide the implant success, the coating should limit platelets adhesion and delay the clot formation. Scientists have focused in many strategies, using chemical and physical modifications, to improving polymers hemocompatibility. One possibility is the introduction of sulfate or sulfonate groups in chitosan chain. This natural polymer has attracted attention due to its potential to act as a biomaterial, to mimic the effects promoted by heparin, one of the most used anticoagulant. Sulfur-containing chitosan, shows the ability to reduce proteins adsorption, decrease thrombogenic properties and limit clot formation. In this context, we produced two types of chitosan membranes: one with chlorossulfonic acid, to form 2,N-3,6,O-sulfated chitosan, and other with 5-Formyl-2-furansulfonic acid sodium salt (FFSA), to obtain Nsulfonated chitosan. The membranes were characterized and its effects over proteins adsorption and platelet adhesion evaluated. 1H-13C NMR and FT-IR analysis confirmed the N and O substitution of chitosan when treated with chlorossulfonic acid, while FTIR and XPS analysis evidenced the sulfonation by FFSA. The 2,N-3,6,O-sulfated chitosan showed decrease in the bovine serum albumin-BSA (36.8%) and fibrinogen (20%) adsorption, and in the platelet adhesion (93.7%), which was observed by SEM images. For N-sulfonated chitosan, a more pronounced adsorption rate was observed at pH 5.0 than at pH 7.4, and the adsorption equilibrium was achieved, in both cases, after approximately 20 min. The platelet adhesion was about 50% lower than that of native chitosan. Afterwards, stainless steel surfaces, commonly used for cardiovascular applications, were coated with sulfonated chitosan, by using dopamine and PEG as anchors, and the effect of these grafted surfaces on platelet adhesion and clot formation were investigated. Surface characterization techniques evidenced that the coating formation was successful, and the sulfonated chitosan grafted sample exhibited a higher roughness and hydrophilicity, if compared to native chitosan one. Moreover, sulfonated surface limited platelet activation and the process of clot formation, thus confirming its high biological performances in blood. In conclusion, this sulfonated-modified chitosan has potential to be used as blood-interacting material. 41

Jayachandran Kizhakkedathu Professor, Life Sciences Institute, The University of British Columbia Email: [email protected]

• • •

Background: Ph.D. (Chemistry), Indian Institute of Chemical Technology, 2000 M.Sc. (Chemistry), Mahatma Gandhi University, India, 1994 B.Sc. (Chemistry), Mahatma Gandhi University, India, 1992

Expertise: • Macromolecular Therapeutics, • Cell-Surface Engineering, • Proteomic Reagents, • Blood-Compatible Surfaces and Devices Awards and honors: • UBC Award for Excellence in Basic Science Research, 2013 • Career Investigator Award, Michael Smith Foundation for Health Research, July 2011 – June 2019 • Excellence in Research and Discovery, UBC Department of Pathology, 2011 New Investigator Award in Transfusion Science, Canadian Institute of Health Research, March 2005 – February 2010

Long Acting Polymer Therapeutics for Systemic Iron Excretion Jayachandran N. Kizhakkedathu Centre for Blood Research, Department of Pathology and Laboratory Medicine, Department of Chemistry, The University of British Columbia, Vancouver, BC, Canada Email: [email protected] Abstract: Advances in biomaterials have greatly benefited modern medicine. Specifically, the design and development of novel therapeutics and materials for tissue replacement has improved the health of millions of people. Focus of my laboratory at the Centre for Blood Research, University of British Columbia (www.cbr.ubc.ca) is to innovate therapeutics for blood disorders and immunological rejection by understanding the molecular level interactions of tailored polymers with biological systems. In this presentation, I will discuss our ongoing effort in the development of polymer therapeutics for the treatment of systemic iron overload. Systemic iron overload occurs in transfusion-dependent hemoglobinopathies such as sickle cell disease and thalassemias, and in acquired anemias such as myelodysplastic syndromes (MDS). Current treatments with Iron (III) specific chelators have significant limitations, including short circulation time, severe toxicity, low efficiency, non-compliance and lack of target organ specificity. I will discuss the design of new long acting therapeutic polymers to specifically bind iron. These therapeutic polymers are designed to provide long circulation, biodegradation and with high iron (III) binding efficiency. The hemocompatibility, cell toxicity, in vivo tolerance and systemic iron excretion of these agents in mouse models will be discussed. References: Abbina, S et al. Hyperbranched polyglycerols: recent advances in synthesis, biocompatibility and biomedical applications. Journal of Materials Chemistry B: Materials for Biology and Medicine 2017, 5, 9249-9277. Imran ul-haq, M et al. Design of long circulating nontoxic dendritic polymers for the removal of iron in vivo. ACS Nano 2013, 7, 10704.

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Dr. Louise M.Jennings Associate Professor of Medical Engineering in the School of Mechanical Engineering,University of Leeds Email: [email protected] Dr Jennings is an Associate Professor of Medical Engineering in the School of Mechanical Engineering. Following a spell in industry Dr Jennings came to Leeds to study for a PhD in cardiovascular bioengineering. Her current research interests now include the area of musculoskeletal interventions in the knee, hip and knee artificial joint replacement and cardiovascular biomechanical function. In all of these areas Dr Jennings’ interests are predominantly experimental, developing functional pre-clinical simulation and research methods that can then be applied to medical devices and interventions. A Chartered Mechanical Engineer with the Institution of Mechanical Engineers, Dr Jennings continues to work closely with industry, applying pre-clinical research methods to medical devices in commercial development in order to enhance their safety and reliability, with established collaborations with DePuy Synthes, Vascutek Terumo, Invibio, Mathys Medical, and Biocomposites Ltd. Dr Jennings also works closely with Simulation Solutions to develop the next generation of physical hip and knee joint wear simulators. Engineering ‘50 active years after 50®’ through multi-disciplinary research, innovation, knowledge creation and translation. L.M.Jennings1 1Institute of Medical and Biological Engineering, University of Leeds, Leeds, UK

Abstract: With a vision of 50 active years after 50® we are dedicated to improving the quality of life of an ageing population. Driven by clinical challenges, in the Institute of Medical & Biological Engineering at the University of Leeds we undertake solution focused pioneering research in medical technology. Specifically, our focus is on longer lasting joint replacements, early tissue sparing and repair interventions, and biological scaffolds for tissue regeneration; supported by computational and experimental simulation systems for design and pre-clinical testing. Our current research activities include the hip, knee, spine and ankle joints as well as cardiovascular devices. With the world’s largest academic capability for experimental simulation and evaluation of joint replacements, early tissue sparing and repair interventions, and cardiovascular devices, complemented with our computational modelling capability, we are able to pre-clinically evaluate potential new treatments and therapies. Such potential new treatments and therapies may be those we have developed ourselves, such as our patented decellularisation methods to produce biological scaffolds for tissue engineering and repair, or through collaborations with industry or other academics. Such pre-clinical assessments should be performed prior to animal studies or clinical trials as they can potentially reduce risk to industry investors, the health service and also patients during introduction of new technology. Our stratified approach to preclinical testing takes into account variations in surgical delivery, variations in kinematics/inputs, variations in the patient population and degradation of the biomaterials technology, and indeed combinations of all these different conditions. Testing under such a wide portfolio of stratified conditions cannot be achieved using experimental simulation alone, as too many experimental simulations are needed. Hence we combine this with a computational modelling approach. Once 43

standardised pre-clinical simulation methods have been developed we incorporate these into international standards, an example is the development of a new hip wear testing standard under edge loading conditions that has been recently been published (ISO14242-4). Through our multidisciplinary approach in combining expertise in science, engineering, innovation and medicine we are uniquely able to translate scientific discovery and research into the treatment of human diseases and disabilities and practical clinical applications.

Pre-clinical Assessment of an All Polymer Knee Replacement Cowie RM1, Briscoe A2, Fisher J1, Jennings LM1 1 Institute of Medical and Biological Engineering, University of Leeds, Leeds, UK 2 Invibio Ltd, Thornton Cleveleys, UK Background PEEK has been considered as an alternative arthroplasty bearing material due to the biocompatibility of its wear debris. In this study, PEEK-OPTIMA™ has been investigated as an alternative to cobalt chrome in the femoral component of total knee replacements . This would give a non-metallic implant of lower weight and would potentially reduce stress shielding. A series of experimental studies of both the patellofemoral and tibiofemoral joint were carried out to investigate the wear performance of this implant. Methods Experimental wear simulation of both the tibiofemoral and patellofemoral joints was carried out using 6 station Pro-sim simulators with input profiles to replicate a walking gait cycle. For all tests, the all polymer knee replacement (collaboration partners Maxx Orthopedics Inc., Plymouth Meeting, PA, USA and Invibio Knee Ltd, Thornton-Cleveleys, UK) was investigated in parallel with a conventional metal-on-polyethylene implant (MAXX Othhopedics Inc.) of similar initial surface topography and geometry. Minimum n=3 carried out for each study. 25% bovine serum supplemented with 0.03% sodium azide was used as a lubricant, the wear of the UHMWPE tibial component was assessed by gravimetric analysis and the articulating surfaces measured using profilometry. Results The wear of UHMWPE in both the tibiofemoral and patellofemoral joints was low, 2.5 µM concentration induced cytotoxicity on HaCaT cells by autophagy mediated apoptotic cell death by increasing autophagy through the formation of acidic lysosomes and autophagosomes, and it was confirmed by elevation in the expression of autophagy regulated proteins Beclin 1 and LC-3B. Furthermore, SeNPs, induced chromatin codensation and nuclear fragmentation was evaluated by DAPI staining. In further mechanistic evaluation SeNPs were found to activate the Akt, WNT and NF-κB signaling pathways along with elevated ROS levels. CONCLUSION: The novel insights of current study are SeNPs induce autophagy mediated apoptotic cell death in human keratinocytes cells by upregulating the autophagy and apoptosis regulated protein expressions in human keratinocytes. KEYWORDS: SeNPs, Keratinocytes, Cytotoxicity, Autophagy, Apoptosis

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CR-7: Chaos and loss of information during progression of oral cancer Reetoja Nag 1,Mousumi Pal 2,Ranjan Rashmi Paul 2,Jyotirmoy Chatterjee 3,Raunak Kumar Das 1,4 1

Centre for Biomaterials,Cellular and Molecular Theranostics,Vellore Institute of Technology, Vellore, India 2 Department of Oral and Maxillofacial Pathology,Guru Nanak Institute of Dental Sciences and Research, Kolkata, India 3 School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India 4 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Australia

BACKGROUND: Diagnosis of oral cancer using digital image processing is becoming increasingly common in case of pathology. As precancerous conditions like Oral Submucous Fibrosis (OSF) initiate in the basal layer of the tissue, it is imperative to study the changes seen in cell nuclei of the basal layer as well the suprabasal layer in Haematoxylin and Eosin stained tissue as they represent histopathologic developments observed during progression of oral epithelial dysplasia. Earlier, shape, size, area, perimeter and aspect ratio of the basal cell nuclei have been studied and have provided clinically important diagnostic information. METHODS: The irregular shape of basal cell nuclei can be compared to fractals which are irregular geometric figures that have the property of self similarity, which is an important principle of chaos theory. Fractal structures are characterized by their fractal dimension. On the other hand, entropy is an important measure which describes the degree of chaoticity and also gives the average rate of information loss about the position of the basal cell nuclei. Entropy can also be used to calculate the quantum de-coherence of the basal cell nuclei in it’s different states during progression of dysplasia. Entropy and fractal dimension are interdependent. Entropy, fractal dimension and quantum de-coherence of the basal cell nuclear features of oral H&E stained images can be calculated in MATLAB or ImageJ Software. RESULTS AND CONCLUSION: The main aim of our study is to successfully segment out the basal cell nuclei in H&E stained images of oral epithelium in normal, OSF(without dysplasia) and OSF(with severe dysplasia) cases and calculate their entropy, fractal dimension and quantum de-coherence. This will provide knowledge about degree of chaocity and information loss during dysplastic changes during progression of cancer which will ultimately help in early determination of neoplasia. KEYWORDS: Oral Submucous Fibrosis, Dysplasia, Chaos, Fractal Dimension, Entropy

CR-8: Gas Phase Cigarette Smoke Extract Alters Cell Morphology and Intracellular Properties of Primary Oral Keratinocyte Ripon Sarkar1, Kabita Chatterjee2, Ananya Barui1 1. Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and technology, Shibpur, India 2. Department of Oral and Maxillofacial Pathology, Buddha Institute of Dental Sciences, Patna, India BACKGROUND: Cigarette smoking is a global health problem and is the major risk factor for the development of oral cancer. Chronic exposure of cigarette smoke leads to the progression of oral squamous cell carcinoma. Cigarette smoke extract (CSE) has a carcinogenic effect on oral keratinocyte cells in vitro. The compounds that present in the gas phase of cigarette smoke 182

extract effects in the biology of oral keratinocyte cells. The aim of this study is to determine the consequence of CSE-treatment NHOK cell viability and imbalance of intracellular biomolecules. METHODS: Human primary oral keratinocyte (NHOK) cells were isolated from normal tissue collected from buccal region of the mouth floor. After reach to 70-80% confluency, NHOK cells were treated with 0, 1, 3 and 5% CSE for 12 and 24 h respectively. Cell morphology, viability and autofluorescence were determined. RESULTS: After treatment with different concentration of CSE, cell morphology was drastically changed. The mean blue, green and red autofluorescence intensity significantly increased in 1% (2.36±0.52, 1.31±0.29 and 2.28±0.14 respectively), 3% (2.55±0.44, 1.93±0.78 and 3.02±0.62 respectively) and 5% (8.82±1.04, 2.99±0.66 and 3.34±0.81 respectively) CSE treated NHOK cells compare to control (1.60±0.66, 1.24±0.31 and 1.65±0.50 respectively). In addition, increased concentration of CSE reduces the percent of cell viability after 12h (95.93±0.75, 73.77±5.28, 66.66±3.59 for 1%, 3% and 5% respectively) and 24h (59.10±16.18, 45.51±7.77, 31.18±6.38 for 1%, 3% and 5% respectively) treatment . CONCLUSION: The study demonstrates that gas phase of CSE affects the cell morphology, autofluorescence property as well as cell viability on primary oral keratinocyte cells. KEYWORDS: Cigarette smoke extract, Keratinocyte cell, Autofluorescence, Cell viability.

THEME- CELL THERAPY CT-1: Induction of Epithelio- mesenchymal transition (EMT) changes by tumour viruses Raunak Kumar Das 1,2, Sourangshu Chakraborty 2, Christopher Ngan 1, Mikaela Cicciarello1, Noel Whitaker1. 1 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney Australia 2 Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology, Vellore, India BACKGROUND: Cancer is a global disease which poses difficult questions for clinicians as well as researchers. There has been extensive research into the mechanisms by which normal cells are tumourigenically transformed (including loss of replication control, anchorage independence, evasion of immunity and ignoring external regulatory signals). Combined with metastasis, this makes cancer cells a deadly disease. Current cancer research pitches EMT as the mechanism at the forefront for endowing a transformed epithelial cell with migratory potential to escape to distant anatomic sites and cause metastasis. Emerging evidence implicates tumourigenic viruses, such as Epstein-Barr Virus (EBV) and Human Papillomavirus (HPV) in initiation of EMT and cancer. The current study aims to investigate the induction of EMT by HPV infected cells. METHODS: The HPV-negative cervical cancer cell line (C-33A) was chosen for this study. C33A was transfected retro-virally with HPV 16 E6/E7 and HPV 18 E6/E7. Control and transduced cells were then analysed for the expression and localisation of β-catenin and c-myc by immunocytochemistry. Furthermore, proteins and RNA were isolated from the control and 183

transduced cells for the analysis of EMT markers and transcription factors such as SNAIL, TWIST and ZEB. RESULTS: Nuclear translocation of β-catenin and increased expression of c-myc was observed in the C-33A transduced with HPV 16 E6/E7 and HPV 18 E6/E7. EMT master regulators were also found to be increased in the transduced cells along with bias in expression of p63 isoforms. CONCLUSION: The observed changes indicate HPV E6 and E7 oncoproteins are associated with the initiation of EMT and, thereby, have an important role in the metastatic transformation of oncogenic virus infected cells. KEYWORDS: EMT, HPV, β-catenin

CT-2: Design and synthesis of N-terminal Labeled undecapeptide: a case study towards the targeting cancer cell 1 Rajavenkatesh K , Aishwarya S 2, PurnaSai K 2, Thennarasu S 1. 1. Organic and Bio-Organic Chemistry Laboratory, CSIR-CLRI (Central Leather Research Institute), Adyar, Chennai-600020, India. 2. Biological Materials Laboratory, CSIR-CLRI (Central Leather Research Institute), Adyar, Chennai-600020, India. Owing to the life style and food habits, cancer has become one of the most dreadful diseases in recent years, and an average of 8.2 million deaths are accounted every year,1 amongst which lung, stomach, liver, colon, and breast cancer are predominant. The majority of interventions for cancer have not been successful due to the reduced bioavailability of drugs, reduced cell membrane permeability to drugs and the toxicity to normal cells leading to several side effects. To mitigate these effects, it is an absolute requirement that the drugs are targeted specifically to the cancer cells. One such delivery system includes design of peptides that can identify cellular epitopes on cancer cells, and there by, differentiate cancer cells from normal cells. We have designed and synthesized a N-terminal labeled undecapeptide and examined its potential as well as biocompatibility for targeting cancerous cells. Interestingly the synthesized undecapeptide displays the following features: (i) surface physical activity, (ii)membrane penetrating ability, (iii) uptake of peptide by the cells and (iv) strong electrostatic interactions with lipid membrane. CT-3- Decellularized human amniotic membrane exhibits protective role under oxidative stress to maintain the cell functionality of cultured ARPE-19 cells. Lekshmi Krishna1,2, Murali Subramani1, Murugeswari Ponnulagu1, Kaushik Chatterjee3, Rohit Shetty4, Debashish Das1* 1. Stem Cell Research Lab, Grow Laboratories, Narayana Nethralaya Foundation, Bangalore, Karnataka, India. 2. School Of Bioscience And Technology, Vit University, Vellore, Tamil Nadu, India. 3. Department Of Materials Engineering, Indian Institute Of Science, Bangalore, Karnataka, India. 4. Department Of Vitreo-Retinal Services, Narayana Nethralaya Eye Hospital, Bangalore, Karnataka, India.

BACKGROUND: Oxidative stress is the leading causes of several retinal aging diseases. Cell therapy using decellularized human amniotic membrane (dHAM) has shown a potential therapeutic role. But the underlying mechanisms for maintaining the physiological properties of 184

transplanted cells and their survival in a diseased milieu has remained unanswered. Hence, the objective was to investigate the potential role of decellularized human amniotic membrane in maintaining the cellular properties of retinal pigment epithelium in an oxidative stress environment. METHODS: ARPE-19 cells were cultured on tissue culture dishes or decellularized human amniotic membrane in hyperoxia conditions. Gene expression, immunofluorescence staining, ELISA assays were performed to investigate the cellular attributes. Assays were performed for assessing the reactive oxygen species, proliferation, apoptosis, and secretion of vascular endothelial factors status. RESULTS: The results revealed reduced reactive oxygen species (p≤0.0001), and apoptosis (p≤0.05) in cells cultured on decellularized human amniotic membrane compared to those on tissue culture in oxidative stress conditions. The secreted vascular endothelial growth factor was significantly reduced (p≤ 0.01). CONCLUSION: These findings provide insights into the underlying mechanism implying that ARPE-19 cells not only grow better but also maintain their physiological properties when grown on decellularized human amniotic membrane in an oxidative stress condition. Keywords: Decellularized human amniotic membrane, retinal pigment epithelium, oxidative stress.

CT-4: Extracellular matrix based three dimensional cell culture systems for in situ cytocompatibility evaluation of select phytochemicals Josna Joseph*, Annie Abraham* Advanced Centre for Tissue Engineering, Dept. of Biochemistry, University of Kerala, Thiruvananthapuram, INDIA Email: [email protected] BACKGROUND: Three dimensional cell culture systems are better biomimetic tissue models over 2D cell culture systems by providing enhanced cell interactions and better simulation of in vivo biological environment. Also, they could act as platforms for drug toxicity and cytocompatibility evaluation. In this context, the main objective of our study is to construct an extracellular matrix (ECM) based three dimensional annulus - core cell culture system for cytocompatibility evaluation of select phytochemicals with tissue regenerative properties. METHODS: A three-dimensional migration assay model was set up in 3% agarose-coated 24well culture plates by surrounding Rat tail vein Collagen I - Genipin cores (1cm diameter) containing 1x105 L929 fibroblast cells/ml with an annular tissue simulant of Collagen-Genipin gel with select tissue regenerative phytochemicals. Cellular migration into the phytochemicalladen inner core was analysed quantitatively by counting cells and measuring the migration distance (n=5 fields) from the gel interface to the farthest cell body (n=3 gels) under phase contrast microscopy. This was further confirmed by the Calcein AM- Ethidium homodimer staining and confocal imaging. Physico- chemical characterisation and phytochemical incorporation in the hydrogels was assessed by X-ray diffraction analysis, FTIR, NMR spectroscopy and gelation time and rheology parameters were also assessed.

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RESULTS: L929 fibroblasts proliferated and migrated chemotactically in collagen-genipin hydrogels. The migration distance and the number of cells migrating into phytochemical laden gel cores was significantly higher in phytochemical incorporated group when compared with the blank control group.The physico-chemical characteristics, gelation time, rheology parameters of the hydrogels were found to be favourable for the cell survival and proliferation within this gels. CONCLUSION: This study proves that this ECM annulus-core three dimensional gel construct could be an ideal in situ cytocompatibility evaluation platform, which could be further developed with different type of cells and drug/ mitogen combinations. KEYWORDS: hydrogels.

3D

cell

culture,

Cytocompatibility,

Phytochemicals,Collagen,Genipin,

CT-5- Heat shock protein 70 mediated myoblast proliferation and differentiation on alginate dialdehyde-gelatin hydrogel system Aswathy M. R1, Roy Joseph2, Praveen K. S1 1. Division of Tissue Culture, Department of Applied Biology 2. Division of Polymeric Medical Devices, Department of Medical Devices Engineering, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura- 695012, Thiruvananthapuram, Kerala,India

BACKGROUND: Heat shock protein (Hsp) performs multiple housekeeping functions in cells. The 70-kDa heat shock protein (Hsp70) assist a wide range of cellular and physiological processes. This includes the folding, assembly and stabilization of newly synthesized proteins, refolding of misfolded and aggregated proteins, membrane translocation of organellar and secretory proteins, and control of the activity of regulatory proteins. The role of Hsp`s are critical in cells surviving in stressful conditions. Emerging evidences indicates the role of Hsp’s in both cardiac and skeletal muscle cells due to their inherent property. In addition it is also reported, Hsp’s may be expressed in cell containing hydrogel system for the cells to perform normal function. METHODS: Present study investigated the role of Hsp 70 in Alginate dialdehyde (ADA) – Gelatin hydrogel system encapsulated with C2C12 mouse myoblast cells. Tunable ADA- Gelatin system was synthesized by cross linking periodate-oxidized sodium alginate with gelatin in the presence of small concentrations of borax which catalyze the reaction. Hsp 70 chemical inhibitor was used to determine and elucidate the effect of Hsp 70 in C2C12 mouse myoblast encapsulated in ADA-Gelatin hydrogel system. The protein level and gene level expression studies were carried out to study the impact. RESULTS: Protein level and gene level expression studies the present study found that ADAGelatin hydrogel system supports myoblast cell proliferation and differentiation in Hsp 70 dependent manner. CONCLUSION:The present study suggests the novel role played by Hsp 70 in myoblast differentiation and proliferation.

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CT-7: Reversible Addition-Fragmentation chain Transfer (RAFT) Polymerization Mediated Cationic Poly(amino acid) for Efficient Gene Carrier Rima Saha, Rapti Chakraborty and Kishor Sarkar* Gene Therapy and Tissue Engineering Lab, Department of Polymer Science and Technology, 92, A.P.C. Road, University of Calcutta, Kolkata- 700009, West Bengal, India * E. mail: [email protected] BACKGROUND: Controlled living radical polymerization (CLRP) is one of the most attractive polymerization technique. Among the various CLRP techniques such as NMP, ATRP etc., RAFT polymerization has been renowned as the most versatile technique due to its compatibility with wide range of functional monomers, reaction media, narrow molecular weight distribution and lack of toxic metals as used in ATRP. From the past decade, gene therapy has become promising modern therapeutic tool to treat incurable genetic diseases from Alzheimer to cardiovascular problems to cancer. Despite the remarkable transfection efficiency of viral vectors, severe toxicity, immunogenicity and high production cost limit its clinical application. Polymeric nonviral vectors have shown extensive interest due to its comparatively low toxicity, ease of production and nonimmunogenicity. However poor transfection efficiency is its major drawback. Due to the nontoxicity of amino acids, herein we hypothesize to develop amino acid based nontoxic nonviral gene carrier by RAFT polymerization method for gene therapy applications. METHODS: In this work, we synthesized a series of polymers with various architectures such as brush, block and graft polymer containing glycine, leucine and phenyalanine amino acids conjugated with hydroethylmethacrylate (HEMA) monomers. The synthesis of polymers was confirmed by FTIR, NMR and DLS. In vitro toxicity was observed on cancer cell such as HeLa, HepG2 and MCF7 and normal cell such as stem cell. DNA complexation capability of the polymer was characterized by agarose gel electrophoresis and ethidium bromide displacement assay. RESULTS: In vitro toxicity shows that the polymer are totally nontoxic toward both cancer cell and normal cell even at very high concentration of 1 mg/ml. The polymer also showed extensively high pDNA complexation capability at very low N/P ratio although it was dependent on amino acid types. CONCLUSIONS: Therefore, the amino acid based polymers may be attractive candidate for gene therapy application. KEYWORDS: Living Polymerization; RAFT Polymerization; Cationic Polymer; Amino Acid; Gene Therapy

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THEME : DRUG DELIVERY DDEL-1: Synthesis and Characterization of Stimuli Responsive Polymer Grafted Organically Modified Mesoporous Silica and its Application in Targeted Drug Delivery Mishra Smrutirekha* and Nebhani Leena¹* ¹Department of Materials Science and Engineering, Indian Institute of Technology, Delhi Hauz Khas, 110016, India *Corresponding author. Tel.: +91 11 2659 6691; fax: +91 11 2659 1421. *E-mail address: [email protected], [email protected] Background: Mesoporous silica is considered one of the efficient material for drug delivery application due to its biocompatibility and uniform pore volume. In order to carry the required drug in the pores it is an important challenge to choose efficient gatekeepers and to graft it onto the surface of mesoporous silica. In the present work, grafting of poly N-isopropyl acrylamide (PNIPAM) was performed via reversible addition-fragmentation chain transfer (RAFT) polymerization. RAFT functionalities were anchored to the surface of mesoporous silica then Nisopropyl acrylamide was polymerized onto the surface of the mesoporous silica. Methods: RAFT functionalized mesoporous silica nanoparticles were grafted with PNIPAM via reversible addition-fragmentation chain transfer polymerization. Results: The resultant mesoporous silica were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), nitrogen adsorption isotherms (BET) and solid state NMR. The PNIPAM can control the access of the guest molecules to and from the mesoporous silica due to its thermoresponsive behaviour. Thus to study the thermoresponsive nature of PNIPAM dye loading experiments were carried out and was finally characterized by confocal laser scanning microscopy. Conclusion: These results suggest that the PNIPAM grafted mesoporous silica can be used for drug loading and release studies. Keywords: Mesoporous silica nanoparticles, RAFT polymerization, organically modified silica nanoparticles, targeted drug delivery.

DDEL-2: Polymer brushes grown cup shaped particle via surface initiated ATRP for triggered drug delivery Ifra* and Saha Sampa¹* [email protected], [email protected] ¹Department of Materials Science and Engineering, Indian Institute of Technology, Delhi Hauz Khas, 110016, India *Corresponding author. Tel.: +91 11 26596692; fax: +91 11 26596692. *E-mail address: [email protected], [email protected] Background: Shape and size of the particles are the physical aspects that play an important role in the release profile for drug delivery applications. Electrohydrodynamic Jetting technique facilitates the fabrication of particles with controllable shape and size only by changing some solution and processing parameters. In present work, we have optimized the fabrication of cup shaped particles of a newly synthesized copolymer and observed the mechanism of shape change 188

with respect to change in solvent system, concentration of solution, flow rate etc. Also, different types of polymer brushes were grown from particle surface, resulting shape switching of the system and opening a new way to triggered drug delivery. Experiments Poly(2-hydroxyethyl methacrylate) (PHEMA) , Poly(poly(ethylene glycol) metharylate) (PEGMA) and Poly(2-(dimethylamino)ethyl methacrylate (PDMAEMA) were grown from cup shaped particles fabricated from a newly synthesized copolymer of HEMA and MMA(PMMA-co-PHEMA) via Electrohydrodynamic Jetting technique. Results: Synthesis of PMMA-co-PHEMA was confirmed by Nuclear Magnetic Resonance Spectroscopy (NMR) and Fourier transform infrared spectroscopy (FTIR). Shape and size of fabricated particles were confirmed by Scanning Electron Microscopy (SEM) and Optical Microscope (OM). Dye loading experiments were also carried out and characterized by Confocal Laser Scanning Microscope (CLSM). Conclusion: These polymer brush grown particles can be used for triggered drug delivery application and also for the adsorption of water soluble proteins and peptides etc. Keywords: Electrohydrodynamic Jetting, cup shaped particles, Polymer brushes, shape switching.

DDEL-3 Nanoengineered dual release graft for pain and inflammation management in osteoarthritis 1 Bavya M C , Vimal Rohan K2, Rohit Srivastava1,* 1. Nanobios Lab, Department of Bioscience and Bioengineering, Indian Institute of Technology Bombay, Mumbai-400076, India. 2. Academy of Medical Sciences, Pariyram, Kerala-670503, India BACKGROUND: Osteoarthritis (OA) is a progressive and degenerative joint disorder caused due to wear and tear of tissue joints and cartilages. The clinical syndrome is manifested by the inflammation of joints and tissue degeneration incapacitating the quality of life. To date, OA is regarded as the common multifactorial arthritis affecting millions of individuals especially women resulting disability in gait and increased agony with age advancement. Current treatment regimens include injection of high dose of steroids, oral intake of steroids and replacement surgeries. Intra-articular injection of heavy doses ultimately leads to severe side effects, whereas, when used topically results in hypersensitive reactions. Oral route due to poor availability cannot unable disease prognosis. Replacement surgeries are the final option with challenges such as low success rate, cost, and prolonged follow ups. To overcome the challenges the work presents nanoengineered dual drug release formulation against OA. METHOD: Osteoarthritic drug, Diacerein was encapsulated in polycaprolactone nanoparticle (PCL NP), whereas, the NSAID aceclofenac in thermoresponsive polymeric nanoparticle chitosan grafted poly-N-vinyl caprolactum (Cs-g-PNVCL NP) incorporated in Cs matrix. The dual drug loaded nanoparticles were further incorporated into biodegradable gel chitosan serving as immobilizing matrix for sustained delivery of drugs to cure OA. RESULTS: Study reports the release of diacerein from PCL Np, and Aceclofenac from PNVCL on application of heat pad. Cs gel served as a good immobilizing matrix and controlled the release of drug onto the target site. (D-PCL, A-PNVCL)Cs was found to be biocompatible to L929 cells. Rheology and inversion tests suggest the smooth extrudability of the gel, the ideal characteristic for intra-articular injection. The in vivo biocompatibility studies in wistar rats 189

demonstrated significant compatibility of the material without causing any hypersensitive reactions to the animals. CONCLUSION: The results suggest the matrix (D-PCL, A-PNVCL)Cs as a promising candidate to be used for curing osteoarthritis. KEYWORDS : Osteoarthritis, Intra-articular, PCL NP, PNVCL NP, Diacerein, Aceclofenac

DDEL-4: Enhanced anti-cancerous activity of dual-drug loaded core-shell nanoparticles composed of metal-free fully alternating copolymer Piyush Kumar Gupta1, Sreenath Pappuru2, Debashis Chakraborty2, Santosh Gupta1, Rama Shanker Verma1 1. Stem Cell and Molecular Biology Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, IIT-Madras, Chennai-600036, India 2. Organometallic laboratory, Department of Chemistry, IIT-Madras, Chennai-600036, India Email id. [email protected]

BACKGROUND Polymeric nanomaterials are commonly used in biomedical applications but little is known about their fate in biological systems. The toxicity of polymeric nanomaterials is largely associated with the material source and their synthesis process. In general, these materials are composed of copolymers which are synthesized by ROCOP using different monomers and organometallic catalysts. The metal contaminants in the resulting product are toxic in nature. To address this problem, the metal-free approach was developed for copolymer synthesis. Further, the resulting copolymer was used to develop novel nanodrug carriers (NDC) for drug delivery application in cancer therapy. METHODS The poly (tBGE-alt-PA) copolymer was synthesized by metal-free approach and physiochemically characterized. Further, copolymer was used to prepare novel NDCs and characterized by several methods such as particle size analysis, zeta potential measurement and encapsulation efficiency etc. In addition, several in vitro biological studies were also carried out. RESULTS AND DISCUSSION The poly (t-BGE-alt-PA) copolymer of 9.3 kDa size was synthesized and found to be fully alternating in nature. The average diameter of the novel NDCs ranged between 200 and 250 nm and showed higher drug encapsulation efficiency for both doxorubicin and curcumin. These NDCs further displayed sustained drug release behavior for both drugs in a defined physiological environment. Moreover, the anti-tumor efficacy was examined and showed higher toxicity on MIA PaCa-2 cells with very low IC50 value. These NDCs also inhibited the proliferation of cancer cells due to arrest in G2/M phase of cell cycle, thereby inducing apoptosis with higher ROS levels and augmented mitochondrial membrane depolarization. CONCLUSION The metal-free poly (tBGE-alt-PA) copolymer was synthesized and used to prepare NDCs showing enhanced anti-cancerous activity. This study will open up novel avenues for applications of this copolymer in other fields of biomedical science. KEY WORDS Metal-Free Synthesis, Alternating Copolymer, Drug Delivery, Combinational Cancer Therapy 190

DDEL-5Degradation of gelatin in various acid conditions and analysis of the components Renju R, Kaladhar K# Department of Pharmaceutics, Amrita School of Pharmacy, AIMS healthcare campus, AmritaVishwa Vidyapeetham, Ponekkara, Kochi, Kerala, India,682041 * for correspondence: [email protected] Background: Gelatin hydrolysis products possess numerous pharmaceutical, cosmaceutical and food industrial applications. Conventional acid degradation procedure employed for the production of gelatin hydrolysis products proceeds in an uncontrolled manner leading to formation of many undesired products. There is a need for optimizing this reaction to reduce the yield of undesired products. The aim of this work is to optimize the conditions for acid degradation employed for hydrolysis of gelatin. Methods: For this gelatin in solution form is first hydrolysed with different acids under varying conditions and the products formed after hydrolysis were evaluated using viscometry and soluble peptide content by biuret method. Detailed characterization of the products of hydrolysis was performed using FTIR, MS, NMR etc. Results: A 10 fold increase in acid concentration resulted in 30-40% increase in time dependant viscosity of gelatin solution in case of a non oxidizing acid while oxidizing acid didn’t produce any significant change in viscosity. The decrease in soluble peptide content after hydrolysis of gelatin was found to be greater for nonoxidising acids when compared to oxidizing acids. These observations suggest that the acid hydrolysis reaction of gelatin proceeds at a slower rate in presence of an oxidizing acid in comparison to a non oxidising acid. To understand the mechanism of hydrolysis the reaction products formed after hydrolysis are characterized using FTIR, MS, NMR etc and the results are presented. Conclusion: Acid hydrolysis reaction of gelatin is optimised to reduce the yield of undesired gelatin hydrolysis products.

DDEL-6: Floating drug delivery system using Diclofenac Sodium as a model drug Krishnapriya M, Vyshma K.V, Megha Hansen, Kaladhar K* Department of Pharmaceutics, Amrita School of Pharmacy,AIMS Healthcare Campus Amrita Vishwa Vidyapeetham, Kochi, Kerala Corresponding author E-mail: [email protected] Aim: The aim of the work is to develop a floating drug delivery system (FDDS) for improving the biopharmaceutical aspects of drugs. Method: The FDDS was developed using HPMC, bees wax, cetyl alcohol and ethyl cellulose for developing the release controlling matrix, poly ethylene glycol as dissolution enhancer and sodium bicarbonate as gas generating agent, using hot melt granulation method and loaded with Diclofenac sodium as a model drug. The composition of the FDDS was optimized with respect to the required floating duration and dissolution properties. It was further characterized using various techniques for granules (angle of repose, bulk density, moisture content, drug content, in vitro dissolution) and for tablet (weight variation, thickness, diameter, hardness, friability, content uniformity and drug release kinetics studies). 191

Result and Discussion: The composition of the FDDS is optimized with respect to the required functional properties such as lag time and floating duration (buoyancy time). Among the various composition tried the one with optimum concentration of poly ethylene glycol and sodium bicarbonate produced the above said required properties and further its dissolution properties are optimized. The final formulation met all the requirements for a FDDS. Conclusion: The optimized FDDS could be used as a platform for the controlled delivery of various drugs from the stomach that can improve stability, biopharmaceutical and pharmacokinetic aspects. The delivery system will be used for incorporating into various technologies. Keywords: Floating drug delivery system, diclofenac sodium, HPMC, biopharmaceutical, drug stability

DDEL-7: A novel and simple technique for separation of liposomes from unloaded drug molecules Joseph Jose, Vignesh Muthuvijayan1 1. Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, India Background Liposomes are lipid bilayer vesicles that are made up of phospholipid molecules. They are also the first nano-scale drug delivery systems, which have been transformed into successful clinical products. Over the past few decades, a great deal of work has gone into innovating, improving, and perfecting, several aspects of the liposomal drug delivery system. Separating the drug-loaded liposomes from the free drug is an integral part of producing liposomal drug formulations. However, very little research has been done to improve this process. Conventionally, this separation is achieved by ultracentrifugation, chromatography, and dialysis. All these methods have their drawbacks, such as cost, scalability and efficiency. The objective of this work is to develop a simpler, cheaper, and easily scalable technique to perform this separation step. Methods Liposomes were successfully synthesised and precipitated out using solvents like ethanol and acetone. The efficiency of this separation was compared to other conventional techniques like ultracentrifugation. The morphological integrity of the precipitated liposomes was confirmed using dynamic light scattering and electron microscopy. The release profile of doxorubicin from these precipitated liposomes was compared to liposomes separated out by other conventional methods like ultracentrifugation. Results This novel precipitation technique was able to separate out liposomes more efficiently than conventional ultracentrifugation. The structural integrity of the liposomes was found to be preserved, even after precipitation. The release profile of doxorubicin was found to be similar in case of precipitated and centrifuged liposomes. Conclusion This method proves to be an efficient, convenient and simpler technique to separate out liposomes from the free drug compared to conventional techniques. 192

Keywords Liposome, Precipitation, Doxorubicin, Ultracentrifugation, Release -profile, Separation

DDEL-8 - formulation and evaluation of betamethasone dipropionate loaded emulgel for cutaneous lupus erythematosus Gayathri P P and Vidhya Viswanad Department of Pharmaceutics,, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham University

Background: Cutaneous lupus erythematosus is a chronic, autoimmune disease that displays many diverse symptoms. Considering the advantages of an emulgel our aim of this work was to formulate a topical emulgel from betamethasone di-propionate loaded nano emulsion for increased sustained release of the drug with decreased side effects. Methods: The pre-formulation studies of the drug and excipients, it was formulated into nano emulsion by aqueous titration method using olive oil as oil phase, tween 80 and ethanol as surfactant and co surfactant and distilled water as aqueous phase, which was optimized and evaluated for physico-chemical characterization and in-vitro drug release studies. Later the prepared nano emulsions were used to formulate emulgel using carbopol 934 and it is characterized and evaluated. Result: Physico-chemical properties of all the formulations were found to be satisfactory. The optimized formulation (F2) shows good spreadability, extrudability and viscosity. The drug content in the formulation was found to be 89.67 %. In-vitro drug release and ex-vivo permeation studies of the optimized formulation (F2) were compared with the marketed formulation. Conclusion: The F2 formulation showed better sustained release with a maximum release of 92.67 ± 0.25 % whereas average cumulative amount of drug permeated was found to be 783.709 µg/cm² at the end of 24 hours.

DDEL-9 Zinc oxide nanoparticle conjugated peptide delivery to mature murine dendritic cells tested by microfluidic single cell level pairing with T lymphocytes Ayan Chatterjee 1, Srijeet Tripathy 1, Milan Sasmal 2, Tapas Kumar Maiti 3, Tarun K Bhattacharyya 4 1. Advanced technology Development Centre, Indian Institute of Technology Kharagpur (joint 1st author) 2. Department of Electronics and Telecommunication Engineering, VPKBIET Baramati Pune 3. Department of Biotechnology and Biochemical Engineering, Indian Institute of Technology Kharagpur 4. Department of Electronic and Electrical Communication Engineering, Indian Institute of Technology Kharagpur

BACKGROUND: Zinc oxide nanoparticles (ZnONps) are cationic in nature. It has been reported that polypeptides upon adsorption to such cationic particles undergo denaturation, thereby facilitating multilayer adsorption also helping easy desorption of higher layer bound polypeptides. We thus hypothesized that the nanoparticle can be employed as efficient carriers of peptides for intracellular delivery to dendritic cells for vaccine development purposes. As proof of concept, we conjugated ova peptide ( SIINEKL) onto ZnONps and introduced them into murine dendritic cells followed by microfluidic single cell level cocultures with OT-1 CD8 T cells. T cell 193

responses were recorded by analysing the calcium signalling patterns and the subsequent quantification of interleukin 2 (IL 2). We used an ova antagonist (SIIGFEKL) and H-2brestricted OVA class II epitope as –ve controls. METHODS: The ZnONPs were synthesized with a narrow size distribution and incubated with target and control peptides in a phosphate buffer solution for successful functionalization. The dendritic cells were isolated from the bone marrow of C57BL/6 mice and the T cells from OT-1 transgenic mice. The moulds of microfluidic device were fabricated in SU8 and casted in PDMS. Cells were captured and paired using hydrodynamic forces. The peptide nanoparticle conjugate system was delivered into dendritic cells before on-chip paring with T-cells to monitor the calcium responses via ratiometric imaging and intracellular IL2 were analysed via immunocytochemistry. RESULTS: The attachment of peptide molecules onto the ZnONPs was then verified by TEM imaging and fluorescence quenching. Higher interleukin content and lower onset delays in calcium flickers compared to –ve controls indicated successful peptide delivery and favourable peptide-MHCII and T cell receptor interactions. CONCLUSION: These results suggest that ZnONPs –peptide conjugate can be used as efficient delivery vehicles in dendritic cell based therapy. KEYWORDS: Zinc oxide nanoparticles, dendritic cells, T lymphocytes, peptide delivery, microfluidics, single cell pairing.

DDEL-10 A Comparative Study of Nano-Stealth Delivery Systems for Luteolin to Mediate Caspase-3 Induction in HaCaT cells Abhishek Sinha, Suresh P.K. School of Biosciences and Technology, VIT Vellore, Vellore, Tamil Nadu, India. BACKGROUND: Luteolin reportedly has a strong dose-dependent antioxidant potential and can induce caspase-14 in HaCaT cells. The objective of this study was to understand/ enhance the apoptotic potential of this naturally derived polyphenol. Luteolin-loaded liposomes and luteolin-loaded PEGylated liposomes were synthesized, characterized and compared against free-luteolin for its apoptotic potential. For the first time, potential of luteolin and its encapsulated version to induce caspase-3 mediated apoptosis was studied. METHODS: Two variants of liposomes (loaded liposome and PEGylated liposome) were synthesized using thin-film hydration technique. The liposomes were characterized for their size, polydispersity and zeta-potential using DLS, while encapsulation efficiency was estimated using RP-HPLC. Morphology of the constructs was visualized using TEM. MTT- based cytotoxic assay was carried out to assess the cytotoxic potential of luteolin and to compare against the two liposomal variants. Caspase-3 induction potential of luteolin in HaCaT cells was studied for the first time based on cleavage of DEVD-pNA and spectrophotometric detection of the chromophore pnitroaniline. 194

RESULTS: HaCaT cells displayed a 2-fold increase in caspase-3 production when treated with luteolin whereas a 6.6 and 6.8-fold significant increase (p 80%) on HeLa cell even at concentration of 150 µg/ml. Gel electrophoresis data confirmed the biding of the functionalized nanoparticle with pDNA. CONCLUSION: The synthesized gelatin based AgNPs may be an efficient nanomedicine for bimodal targeted therapy of HCC patients. KEYWORDS: Green synthesis, Gelatin, Silver Nanoparticles, RGDS, Cancer Therapy, Nanomedicine

NTECH-19: Synthesis and characterization of genepin cross-linked gelatin coated magnetic nanoparticles for hyperthermic therapy Amy Sarah Benjamin [1], Arunai Nambi Raj N [2], Sunita Nayak [2] [1]

[2]

Department of Physics, School of Advanced Sciences, VIT University, Vellore -6320014 Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), VIT University, Vellore6320014

Customisation of drug design using natural polymers plays an important role in biomedical applications. Magnetic nanoparticles (MNPs) in combination with biopolymers have shown potential application in drug delivery system for cancer therapy. This study is focused on gelatin-coated iron oxide magnetic nanoparticles synthesized by sol-gel method. Genepin was 212

used to crosslink the MNPs. Doxorubicin (DXR) was used as a model drug to evaluate their potential as a carrier system for MNPs. The stability and crystalline nature of the particles was evaluated by Zeta potential and X-ray diffraction analysis. FTIR was used to evaluate the functional group. The drug loading efficiency and drug release kinetics was studied. Hyperthermic and VSM results shows that the gelatin coated magnetic nanoparticles have potential application for anti-cancer treatment. KEYWORDS: Biopolymer, Genepin, Hyperthermia, Magnetic nanoparticles

References: 1.

Wu M, Huang S. Magnetic nanoparticles in cancer diagnosis, drug delivery and treatment. Molecular and Clinical Oncology. 2017; 7(5):738-746.

2.

Gaihre B, Khil MS, Lee DR, Kim HY. Gelatin-coated magnetic iron oxide nanoparticles as carrier system: drug loading and in vitro drug release study. Int J Pharm. 2009 Jan 5;365(1-2):180-9.

THEME : THERAPUTICS THER-1: Radiomics analysis of MRI images in Astrocytoma and glioma patients Venkatesan K1,3, Dr Jomon Raphael1 C, Dr Mathew Varghese K1, Dr Paul Gopu G1, Sivakumar P1, Minu Boban1, Dr Arunai Nambi Raj N2, Dr Senthilnathan K3, Dr Ramesh Babu P3 1 Amala Institue of Medical Sciences, Amala Nagar, Thrissur, India-680555 2 Centre for Biomaterials,Cellular and Molecular Theranostics (CBCMT), VIT, Vellore-632014, India 3 Department of Physics, School of Advanced Sciences, VIT University, Vellore 632 014, India Introduction: Radiomics can provide the information of tumor phenotype with non invasive method. Evaluation and analyses of specific features from tumor images can be correlated for specific tumors. In this study, we evaluated brain tumor related features by using magnetic resonance imaging(MRI). Materials and Methods: 5 astrocytoma and 3 giloma patients with T2 weighted fluid attenuated inversion recovery(T2 FLAIR) MRI images were analyzed with 828 radiomic features by using 3D slicer® software. At first, the MRI images were imported to radiotherapy planning system (Eclipse®) and contours of gross tumor volume (EC) were drawn. Then the images were exported to 3D Slicer and radiomic features were extracted. Secondly, the MRI images were directly imported to 3D slicer and Automatic segmented contours of GTV (SS) were created by using level tracing methods. The radiomics features including Grey level difference method (GLDM), grey level co-occurrence matrix (GLCM), shape related features, first-order information, Grey level run-length matrix (GLRLM), Grey level size zone matrix (GLSZM), neighborhood gray-tone difference matrix (NGTDM) and wavelet based features were extracted for both EC and SS . Extracted features were arranged in 28x30 array matrix and it was imported to matlab® to plot 2 dimensional image mesh. Results: Mean value of max 3D diameter was 9.02cm (SD 1.2cm) and 9.62cm (SD 1.45cm) for EC and SS in astrocytoma. For glioma, it was 9.14cm (SD 2.19)[EC] and 8.54cm(SD 213

2.2cm)[SS]. Mean of total energy was 8.15x10^10[EC] and 7.11x10^10[SS] for astrocytoma. For glioma, it was 3.07^10[EC] and 2.25^10[SS]. Mean energy was 4.217[EC] and 3.796[SS] for astrocytoma. In glioma, mean energy was 3.766[EC] and 3.31[SS]. Mean kurtosis was 5.126[EC] and 4.43 [SS] for astrocytoma. For glioma, it was 4.51[EC] and 5.19[SS]. 2D image mesh was showing small significant variation between these two methods and tumors. Conclusion: Radiomics is prominent tool to extract and analyse the information from the radiological images. In our study, we found that, the variation between tumors can be analyzed and segregated by using radiomic features. Imaging modality and image variation with the equipment is paramount to compare and analyse in the field of radiotherapy.

Fig1: GTV delineation in Eclipse

Fig2: GTV Segmentation in3D Slicer

THEME: STEM CELL 214

STMC-1: DOR agonist (SNC-80) exhibits anti-parkinsonian effect via downregulating UPR/oxidative stress signals and inflammatory response in vivo Erfath Thanjeem Begum Ma, Dwaipayan Sena*. a Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India, Background: The pathophysiology of Parkinson’s disease exhibit imperative roles in unfolded protein response stress-induced oxidative stress and inflammation in general. Although, delta opioid receptor (DOR), has been found to represent anti-parkinsonian effect at behavioral level, its underlying mechanism remains elusive till date. In the present study the role of DOR agonist, SNC-80 and the consorted molecular mechanisms, which translates to behavioral recuperation, has been delineated. Methods: In order to mimic PD, mice were intra-peritoneally injected with MPTP, following exposure to SNC-80 and L-DOPA to elucidate amelioration of the MPTP-induced behavioral impairments. Results: The results obtained suggest that the severity of the compromised motor functions upregulated the UPR stress sensors: IRE-1α/Bip/CHOP, oxidative stress along with the proinflammatory cytokines: IL1β/IFNγ/TNFα and IL-6. These inimical factors combined, aids the persistence of the disease in MPTP intoxicated mice. Supplementation with SNC-80 significantly improved motor functions via down-regulation of the UPR stress sensors and inflammatory cytokines. Additionally, SNC-80 could upregulate Nrf-2 and Heme oxygenase-1 (HO-1) protein expression indicating their involvement in SNC-80′s potential anti-oxidant function. There was also a significant reduction in protein carbonyl content indicating the positive role of SNC-80 in dampening MPTP induced oxidative stress. Concomitantly, L-DOPA also demonstrated an enhanced effect towards improvement of motor functions but did not suppress the UPR and inflammatory responses caused due to MPTP intoxication. Conclusion: Hence, these results suggest that SNC-80 could hold a pivotal role in replenishing motor functions essentially via regulating UPR and inflammation. Keywords: Delta opioid receptor (DOR); SNC-80; unfolded protein response (UPR); antiparkinsonian effect; neural protection; inflammation

STMC-2: Effect of DOR activation on crosstalk between oxidative stress by the inhibition of PARP-1 and inflammatory response in vitro model of PD Erfath Thanjeem Begum Ma, Dwaipayan Sena*. a Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India. Background: Parkinson’s disease (PD) is a multifactorial neurodegenerative disease leads to the loss of dopaminergic neurons. Oxidative stress plays an important role in PD etiology. Delta opioid receptor (DOR) activation has been reported to attenuate oxidative stress, thereby increasing cell survivability.However, the molecular mechanism towards increased neural protection against oxidative stress remains elusive.Inthis study, the role of DOR activation in attenuation of PARP-1 and pro-inflammatory cytokines under the oxidative stress has been investigated.

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Methods: Hydrogen peroxide (H2O2)-induced oxidative stress by the culturing of SH-SY5Y cells in conditioned media from macrophages, which were then treated with LPS, followed by the SNC-80 (DOR agonist), Naltrindole (DOR antagonist), and PARP-1 inhibitor in order to determine the DOR activation. The underlying mechanism of activation of various pathways would be determines using techniques like PCR and immunoblot analysis. Results: The results determine that DOR-activation and PARP-inhibition show similar effect in increasing cell survivability against the H2O2induced oxidative stress, significant additive effect was not observed on combining the two factors. Down regulated PARP-1 levels were observed with DOR-activation against oxidative stress. Increasedexpression of pro-inflammatory cytokines such as Il-1β, IFN and IL-10 upon oxidative stress from macrophages were attenuated significantly by DOR activation. Conclusion: The potential neuroprotective role of DOR activation in neuronal cells against the oxidative stresswas plausibly via attenuation of PARP-1 and pro-inflammatory cytokines. Keywords: DOR, PARP-1, oxidative damage, inflammatory response, DNA damage

STMC-3: The novel miRNAs regulated with DOR activation against ER stress induced cellular model of Parkinson’s disease (PD) in SH-SY5Y cells. ErfathThanjeem Begum Ma, Himadri Basua, Venkatesh Kataria, Dwaipayan Sena*. a Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India. Background: Micro RNAs (miRNAs) are the small non-coding RNAs, that bind to 3’untranslated region (UTR) of messenger RNA (mRNA) to induce degradation of mRNA and regulate specific gene expression. The dysregulatedmiRNAs in Parkinson’s disease have been identified with regards to an increase in their therapeutic target. Our previous study has shown regulation of miRNAs upon delta opioid receptor (DOR) activation against in an in vitroER stress-induced PD model. The current study elucidates the novel miRNAs and its regulation in the same model. Methods: Novel miRNAs were identified from the sequences not showing hits with known miRNA's in the three samples Control, Tunicamycin(Tm) and DADLE+Tm. Differential regulation and target gene regulated pathways were identified. Results: Based on the identified novel miRNAs, 176 miRNas in control, 155 in Tm and 55 in DADLE+Tm were observed individually. The DGE analysis between three samples and its target prediction analysis suggest that DOR activation against ER stress were predicted.Target prediction analysis suggested that many of the novel miRNAs in 2vs3 samples showed an increase in target genes of MAPK, TGF-β, NGF etc. Conclusion: These novel miRNAs has shown to regulate the cell survival and cell death related pathways upon activation of DOR. Hence DOR activation at cellular and molecular level may shed light into a multitude of therapeutic innervations. Key words: Novel miRNAs, DOR activation, cell survivability, Parkinson’s disease, ER stress.

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STMC-4: Amelioration of Acetaminophen-induced liver injury in vivo using delta-opioid receptor activated human mesenchymal stem cells Madhubanti Mullicka, Pearlin Hameedb, Moghal Erfath Thanjeem Begumc and Dwaipayan Sena*. a

Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT) University, Vellore 632014, Tamil Nadu, India,

Background: Intoxication caused due to Acetaminophen remains to be a persistent factor for acute liver injury. Liver transplantation in such cases remains hindered due to the lack of donors. Human umbilical cord blood-derived mesenchymal stem cells (hMSCs), known for their regenerative and reparative potential therefore would be a t treatment target. But the paucity of hMSCs usage is marked by their poor viability post-transplant. Hence, in the current study hMSCs pre-treated with Delta-opioid receptor (DOR) agonist SNC-80 (which corroborates with our previous in vitro studies) have been used in order to enhance the survivability of the hMSCs post-transplant in an in vivo mouse model. Methods: Intraperitoneal injection of acetaminophen at a concentration of 500mg/kg was administered to induce the transient liver injury. Tail vein route of hMSCs with or without SNC80 treatment was given,24h post-injury, which served as a cue for homing of the hMSCs. Determination of acute and chronic liver injury, along with the expression levels of various inflammatory cytokines were analysed upon sacrificing the animals on Day1 and Day15. Various molecular techniques like RT-PCR, as well as immunoblot analysis were used to ascertain the results. Results: The efficacy of the transplanted hMSCs was determined with the induction of the liver injury. According to the hypothesised concept, DOR-activated hMSCs would result in a significant better survival rate as compared to the hMSCs without SNC-80 treatment and also in comparison with the PBS-treated control group. The regulation of the inflammatory cytokines is another aspect that would be ofsignificance, considering the anti-inflammatory and anti-oxidant potential of DOR-activation on hMSCs. Conclusion: In vivo transplantation of the DOR-activated hMSCs would essentially result in an up surged hMSCs viability at the site of liver injury. The transplantation of DOR pre-conditioned hMSCs would necessitate further delineation, to be established as a potential alternative to liver transplantation. Keywords: Human mesenchymal stem cells,Delta-opioid receptor, Acetaminophen, Liver injury, Inflammation STMC-5: Invitro transdifferentiation of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSC) into corneal/limbal epithelial cells. Santhosh K1, Sachin S2, Sreenivasa Rao P1 1. Stem cell laboratory, Department of Biotechnology, National Institute of Technology, Warangal, India 2. Prof. Brien Holden Eye Research Centre, LV Prasad Eye Institute, Hyderabad, India. BACK GROUND: Bilateral limbal stem cell deficiency is the disease caused by loss of limbal stem cells in both the eyes. The treatment for it includes transplanting the cultured cells from the allogenic donor which is having drawbacks of graft rejection and continuous use of antibiotics for long term. The other ways includes differentiation of the oral mucosal epithelial cells, induced pluripotent stem cells or Mesenchymal Stem Cells (MSCs) like adipose stem cells, bone 217

marrow stem cells, and dental pulp stem cells into limbal stem cells. These all cells having disadvantage that cell yield depends on donor age and the procedure is invasive. Other choice of cells which are largely abundant and the procedure is noninvasive are human Umbilical Cord Mesenchymal Stem Cells (hUC-MSC). The objective of the paper includes characterization of hUC-MSCs, checking their inherent property to differentiate into corneal/limbal epithelium and stimulating them to transdifferentiate into corneal/limbal epithelium. METHODS: Cells were isolated using explant method than they are checked for various MSC markers like CD73, CD90, CD105, Vimentin and their differentiation capability into adipogenic and osteogenic cells. The hUC-MSCs were checked for epithelial characteristics by Immunofluorescence studies and transdifferentiation ability using conditioned media approach RESULTS: The hUC-MSCs were positive for MSC markers CD73, CD90, CD105, and vimentin. The cells are also expressing the Pax-6, Wnt7a, CK-8/18 but not CK-3/12 which is specific to corneal epithelium. After conditioned media treatment the hUC-MSCs are changed their morphology and also expressed corneal epithelial specific marker CK-3. CONCLUSION: The hUC-MSCs inherently possess few characteristics of corneal epithelial cells and by using conditioned media we can transdifferentiate them into corneal epithelial cells invitro. KEY WORDS: Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSC), corneal epithelium, limbal stem cells, trans differentiation, adipocytes and osteocytes.

STMC-6: Nucleostemin a prognostic marker of impaired stemness during in vitro expansion of Infrapatellar fat pad derived stem cells Subathra Radhakrishnan1, Shanmugapriya Sellathamby2, Catherine Ann Martin1, Mettu Srinivas Reddy1 and Mohamed Rela1 1. 2.

National Foundation for Liver Research (NFLR), Gleneagles Global Health City, Chennai-100. Department of Biomedical Science, Bharathidasan University, Tiruchirappalli, TN, India

Background: Adipose tissue derived Stem cells (ADSCs) are emerging as a promising source of autologous stem cells used for cell-based therapy. For the past two decades numerous studies demonstrated the differentiation potential of ADSCs and proved they have widened plasticity. Unlike Embryonic Stem Cells (ESC) ADSCs have limited Population doublings. In addition, ADSCs while undergoing differentiation lose its stemness, gain some specific gene expression and become more specialized cells with appropriate functions. Methods: The present in vitro study investigates the implicit significance of passages (P) on the differentiation protocols of Adipose-Derived Stem cells (ADSCs), and the possibilities of predicting the same using expressed markers. We studied ADSCs isolated from the Infrapatellar fat pad (IFP) resected during knee Arthroplasties (IFP-SCs). These cells from Stromal Vascular Fraction (SVF) were subjected to serial passaging to P>6. IFP-SCs at each stage were investigated to predict the suitable passage with stemness characteristic for an appropriate differentiation protocol or cell-based therapies. Results: We report that Nucleostemin, a GTPase marker of stem cell proliferation shuttles between nucleolus and nucleoplasm, vanishes prior to terminal differentiation. We report that prolonged culture of IFP-SCs leads to the loss of the stem proliferative marker Nucleostemin 218

(NS) a GTPase that shuttles between nucleolus and nucleoplasm disappears both in nucleolus and nucleoplasm and acquires neurogenic phenotype. Conclusion: Prolonged culture of IFP-SCs lead to the loss of the proliferative marker NS in both nucleolus and nucleoplasm and development of a neurogenic phenotype. It is recommended that for both differentiation protocols and cell-based therapies IFP-SCs from early passages are used. NS, both its intensity and sub-cellular localization, may be useful as a dynamic marker for Stemness.

STMC-7

RNF213-a plausible link between adipogenesis and angiogenesis Priyanka Sarkar, Kavitha Thirumurugan* Vellore Institute of Technology, Vellore *[email protected]

Background: Ring finger protein RNF213 (alias: mysterin, E3 Ubiquitin transferase/ligase), a gene responsible for Moyamoya disease is mainly involved in angiogenesis and protein-protein interactions. Adipocytes being the most vascularized tissues in the body express a number of angiogenic factors like VEGF, FGF, TGF-β and anti-angiogenic factors like TSP, endostatin, angiostatin. Biochemical and functional analysis of RNF213 exposed its dual functionality, one as an AAA+ ATPase and another as E3 ubiquitin ligase. But its role in adipogenesis process is not yet explored and therefore this study revolves around this idea. Methodology : To know the expression of RNF213 in adipocytes, human protein ATLAS was explored and in silico analysis was performed by searching the literature for microarray data to study the genes regulated during adipogenesis. Then the fold change of RNF213 was measured using qRT-PCR, at pre-adipocyte and mature adipocyte stages. Mouse 3T3L1 adipocyte cell lines were obtained from NCCS, Pune. Complementary DNA was obtained from the cultured cells and its mRNA was analyzed for gene expression of RNF213 at the preadipocyte stage (day -2 to day -1), on the day of initiation of differentiation (day 0) and mature adipocyte stage (from day 2-6). In order to characterize and observe whether RNF213 can play a role in adipogenesis, its domain structure was found using INTERPRO. Following this, separate domains of RNF213 as well as the complete protein were used to analyze the interacting partners and the plausible biological processes and molecular functions through STRING. The plausible pathways were elucidated through STRING and verified with KEGG. Results: RNF213 has the following domains: P loop NTPase, AAA+ ATPase, Znf RING/FYVE/PHD, Znf RING type, Znf C3HC4 RING-type. Some of the interacting partners are PTP1B, UBA7, atg, HERC. The biological processes mainly involved are catabolic and metabolic processes, protein modification and protein ubiquitination. In addition, the molecular function includes ligase activity, beta and microtubule binding activity, transferase activity and ubiquitin ligase activating activity. The pathways followed were listed as regulation of autophagy, FOXO signaling pathway, ubiquitin mediated proteolysis and renal cell carcinoma and HIF signaling pathway. In silico analysis of 3T3L1 mouse cell line microarray data reveals that RNF213 is chiefly expressed at the time of initiation of adipocyte differentiation. Furthermore, this has been validated by real time gene expression analysis which showed a five-fold increase in the mRNA 219

level of RNF213. The expression pattern of RNF213 indicates its role in differentiation of adipocytes. Conclusion In silico results show the role of pathways- FOXO, autophagy and HIF in angiogenesis as well as adipogenesis. Also, RNF213 gene expression at initiation of adipocytes differentiation was observed. Key words RNF213, adipogenesis, angiogenesis, Moyamoya disease

STMC-8 Small molecule mediated osteogenic of human Mesenchymal stem cells for segmental bone defects. Pearlin Hameeda, Geetha Manivasagama, Dwaipayan Sena *. a Centre for Biomaterials, Cellular & Molecular Theranostics (CBCMT), Vellore Institute of Technology, Vellore, India – 632014. Background: Segmental defects of bones results from a variety of aetiologies like high energy trauma, diseases, developmental deformities, revision surgery and resection of tumour or osteomyelitis, which cannot be healed without intervention. BMP 2 is a well-known subclass of transforming growth factor family which induces osteogenesis in pluripotent stem cells. However in 2015, FDA issued a cautionary warning for use of BMP 2 as it causes life threatening effects such as ectopic bone formation and soft tissue swelling. Thus, an alternative drug with the potential to induce osteogenesis in progenitor cells is required. Method: Human Mesenchymal stem cells (hMSCs) were treated with small molecules, Tacrolimus, Purmorphamine and mTor inhibitor (Ku-00063794) in various combination to examine their osteogenic potential and the synergistic effect on hMSCs. The cytotoxicity of small molecules was assessed by MTT and their osteogenic potential by ALP activity, gene expression and alizarin red staining keeping BMP 2 and osteo induction media (OS) as control. Results: Small molecule treatment in combinations, and with BMP 2 and OS did not had any significant cytotoxic effect. The combination of all small molecules with BMP-2 or OS showed a significant increase in the ALP activity at day 4 and 7 than BMP-2 or OS alone; The combination of each small molecule with BMP2 and The amalgamation of all three with BMP-2 or OS showed a significant increase in the gene expression of Runx2, Collagen 1, Osteocalcin and Osteopontin at day 4 ,7 and 21 than BMP-2 or OS alone. After 21 days of culturing a positive alizarin red stain was observed in all conditions. Conclusion: These results suggest the potential use of small molecules in combination have the potential to upregulate osteogenesis in hMSCs and can be exercised in bone regeneration for the treatment of segmental bone defects. Keywords:- Small molecules , hMSCs, BMP-2, osteogenesis

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STMC-9 Activation of Hu-MSCs with DOR agonist SNC-80 can promote cardiomyocyte differentiation, angiogenesis and anti-inflammation L. Vinod Kumar Reddy, Dwaipayan Sen Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT) University, Vellore 632014, Tamil Nadu, India. Background: Cardiac disease remains the leading cause of death in the world. stem cells have become attractive tools for cell therapy and regenerative medicine. Therefore, in the present study we are demonstrating the effect of cardiomyocytes differentiation, anti-inflammation and angiogenesis upon the activation of Delta Opioid Receptor agonist (SNC-80) on Human umbilical cord mesenchymal stem cells (Hu-MSCs) with the combination of small moleculesZebularine, Activin-A, Oxytocin. Methods: The dishes were coated with the gelatin to create the extracellular matrix environment (ECM). Hu-MSCs (15×103) were treated with Zebularine (10nM), Activin-A (10ng), Oxytocin(100nM) alone and with the combination of SNC-80 (1uM) for 7 and 21 days. RNA and Protein was Isolated from respective days and studied the expression of early and late cardiomyocyte specific markers. the spent media has collected from 7 and 21 days, used for angiogenesis, anti-inflammatory cytokine studies and to study the effect of in vitro endothelial tube formation assay. Results: In comparison to the alone with Zebularine, Activin-A, Oxytocin with the combination of SNC-80 has shown more cardiomyocytes differentiation effect. Early, late cardiomyocyte markers (GATA4, GATA6, Nkx2.5, MEF2C and TnT2, Connexin43, CTF1, Desmin, KCNJ2, Myh), angiogenic markers (VEGF, angiopoitin-1, PECAM, vwf) and anti – inflammatory markers (IL-4, IL-10, IFN-γ, TGF-β) are significantly upregulated with the combination of SNC80. Conclusion: Compared to the small molecules alone, with the activation of DOR have shown more cardiomyocyte differentiation and angiogenesis effect and the anti – inflammation. which represents, it might be potential therapeutic strategy for the cardiac tissue regeneration by increase angiogenesis effect and antiinflammation. Key words: Hu-MSCs, DOR, angiogenesis, anti-inflammation, Tissue regeneration, stem cell therapy STMC-10 Secretome of human umbilical cord stem cells - therapeutic effects Durai Murugan Muniswami, Dwaipayan Sen Centre for Biomaterials, Cellular & Molecular Theranostics (CBCMT), Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India. Correspondence Email: [email protected] Background: Stem cell-based therapy holds a great promise for various diseases. Among different source of MSC, human umbilical cord stem cells have advantage of less ethical issues and primitive cells as compared to adult MSC. These stem cell secretome has wider therapeutic application in many neurodegenerative diseases, which has to be elucidated. Methods: Human umbilical cord tissue obtained from the hospital with the informed consent of parents.Tissue explant of cord lining (CL) and Wharton jelly (WJ) MSC were cultured in MEM221

alpha, 10% FBS with 1% antibiotics. Cultured cells were characterized for classical MSC markers by flow cytometry and for the expression of pluripotency (SOX2, Oct4, Nanog, Klf4, cMyc) as well as for the germ layer expression. Stem cells were screened for the expression of trophic factors (BDNF, GDNF, CNTF, NT3, NT4, NGF, FGF1, FGF2, VEGF, HGF, IGF, TGFβ, EGF, PlGF), pro-inflammatory (IFN gamma, TNF α, IL-1b) and anti-inflammatory chemokines (IL-4, IL-10, IFN α1, TGF-β). Results: Human umbilical cord- CL and WJ stem cells expressed CD29, CD73, CD90, CD105 and negative for hematopoietic (CD14, CD45 and CD34) and endothelial marker (CD31, CD106). Both the cells express Sox2, Nanog, Oct4, Klf4, c-Myc with lesser amount, which is responsible for “stemness”. These cells did not express mesoderm marker (NKX2.5, GATA4), but expressed ectoderm (NCAM, nestin) and endoderm germ layer marker of alpha feto-protein (AFP) to a lesser extent. Gene expression studies showed the expression of NT3, TGF-beta, IGF, FGF1, HGF, NT-4, EGF, FGF2, placental growth factor (PLGF), NGF, VEGF, GDNF and BDNF, except CNTF. In addition, various cytokines like IL-4, IL-8, IL-3, IL-7, IL-6, IFNα, TGF-β, TNFα, IL-1b were seen, except IFN-gamma and IL-10. These factors may have therapeutic role in neurodegenerative disease. Conclusion: Stem cell secretome has therapeutic role in many diseases.

STMC-11 Three dimensional polymeric matrices encapsulating mesenchymal stem cells for augmenting the differentiation in to Islet like clusters. Jijo Wilson J1, Prabha D. Nair1 1.Sree Chitra Tirunal Institute for Medical Sciences and Technology (SCTIMST), BMT Wing, Trivandrum, kerala Background: Pancreatic beta cells are responsible for producing insulin which is important for maintaining glucose homeostasis in the body. Diabetic mellitus is a metabolic disease in which there are high blood sugar levels over a prolonged period. This is due to either pancreatic beta cells not producing enough insulin or the cells of the body not responding properly to the insulin produced. Current treatment strategies include dietary management, maintaining physical activity, oral medications, insulin use via injections or pump and islet transplantation. Among these clinical islets transplantation is a promising approach for the treatment of diabetes mellitus. However this method is limited due to the shortage of islets sources. A possible solution to this problem is through regenerative medicine. The development of a bioengineered pancreas by appropriate combination of cells, biomaterial scaffolds and biologically active molecules could provide an alternative avenue for diabetes therapy. Methods: A three dimensional polymeric scaffold was prepared and bio moieties was incorporated to favor the cell adhesion. Mesenchymal stem cells were differentiated in to islet like clusters on this three dimensional scaffold and various in vitro studies are carried out to check the insulin releasing potential of the tissue engineered construct. Results: It was found that the 3D constructs which is prepared by the combination of cells, bimolecules and scaffold mimic native pancreatic islets. Conclusion: This study presents the potential application of tissue engineered pancreatic construct over the current treatment strategies of diabetic mellitus.

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Key words: Islet transplantation, diabetic mellitus, regenerative medicine, mesenchymal stem cells.

STMC-12 Nanopatterned Honey Silk Fibroin Matrix Provides Mechanical Clues for Mesenchymal Stem Cells Bagchi S1, Chaudhary A1, Mukhopadhyay A1, Dhara P2, Rajput M1, Barui A3, Mukherjee R2, Chatterjee J1 1. Laboratory of Medical Imaging and Theragnostics, School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, 721302, India 2. Instability and Soft Patterning Laboratory, Department of Chemical Engineering, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, 721302, India 3. Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, Howrah, West Bengal, 711103, India

BACKGROUND: The use of extracellular matrix (ECM) for tissue engineering applications appears to trigger the regeneration process in an isolated cell lines from their natural ambience. This regeneration requirement of cells into tissues led us to bio-functionalization of matrices by both chemical and textural modification of silk fibroin (SF). Amongst several biomaterials, SF has been widely used owing to its low immunogenicity and good mechanical strength in spite of its poor biological activity and biodegradation rate. METHODS: In this study, we have used Jamun honey (JH), a natural wound healing agent having highest antioxidant potential when compared with 17 other varieties. The micro-dome nano-patterned Jamun honey blended silk fibroin thin films (JHSF) fabricated using soft lithographic technique were further characterized by Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), X-ray Diffraction Spectroscopy (XRD), Differential Scanning Calorimetry (DSC) and Fourier Transform Infrared Spectroscopy (FTIR). In vitro swelling and degradation studies and cell viability (MTT), Immune Cyto Chemistry (ICC) assays were also carried out. RESULTS: XRD, FTIR, DSC peaks were found due to presence of honey and silk fibroin crystals in the thin film. Nanopatterned structure were characterized by SEM and AFM studies. Nano-patterned micro-dome surface structure thin film were found to be biocompatible after in vitro culture of HaCaT, mesenchymal stem cell and A431 cancer cells. The anisotropicity of nanostructures depicted pattern directed mobility of the cells along the voids between the thin film surface structure and eventually grabbing of the micro-dome shaped structure by cellular microfilaments. CONCLUSION: These results suggest that the JHSF can be potential candidate further for tissue engineering and wound healing applications. KEYWORDS: Honey, Silk fibroin, Thin film, Nanopattern

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STMC-13 Biocompatibility of electrospun PVA-honey nanofibers on umbilical cord derived mesenchymal stem cells. Ankita Das1, Ripon Sarkar1, Ananya Barui1 1. Centre for Healthcare Science and Technology, IIEST, Shibpur, India. BACKGROUND: Poly-(vinyl alcohol) (PVA) is a water soluble, biocompatible, polymeric material with excellent physical properties. It has been used for various tissue engineering and regenerative medicine in biomedical applications, owing to its low immunogenicity and good mechanical strength. With the addition of a natural material such as honey, the biocompatibility of the PVA can be further improved. Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal cells located in different adult tissues. Human umbilical cord tissue is considered a rich source of MSCs. Umbilical cord derived MSCs are best suited for tissue regeneration due to its tissue repairing function. The aim of the study is to mimic a suitable niche for umbilical cord derived MSCs. METHODS: The concentration of PVA blended with honey in different ratios (0.2–1%) was electrospun to obtain nanofibers. Nanofibers were characterized with scanning electron microscopy (SEM) and the wettability of the nanofibers were determined by contact angle measurement. Furthermore, the cell viability of umbilical cord derived MSCs on nanofibers were confirmed by SEM and cell viability assay. RESULTS: The morphology of the PVA-honey electrospun nanofibers were observed with the help of scanning electron micrographs . With increase in concentration of honey, the fiber diameter as well as wettability was increased. Furthermore, incorporation of honey with PVA showed increased cell viability. CONCLUSION: Considering the huge demand of stem cell therapy in the field of regenerative medicine, it is very much essential to maintain functional capacity of stem cells. The above results suggest that PVA-honey blended nanofibers can be used as a suitable biocompatible material for ex vivo stem cell expansion. KEYWORDS: Regenerative medicine, Electrospun nanofibers, Mesenchymal stem cells, Scanning electron microscopy, Contact angle, Cell viability STMC-14: The delta opioid peptide DADLE represses hypoxia-reperfusion mimicked stress mediated apoptotic cell death in human mesenchymal stem cells in part by downregulating the unfolded protein response and ROS along with enhanced antiinflammatory effect. Madhubanti Mullicka, Dwaipayan Sena*. a

Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT) University, Vellore 632014, Tamil Nadu, India,

Email: [email protected] Background: Hypoxia-reperfusion (H/R) emblems a plethora of pathological conditions which is potent in contributing to the adversities encountered by human mesenchymal stem cells (hMSCs) in post-transplant microenvironment, resulting in transplant failure. D-Alanine 2, Leucine 5 Enkephaline (DADLE)-mediated delta opioid receptor (DOR) activation is wellknown for its recuperative properties in different cell types like neuronal and cardiomyocytes. In the current study its effectiveness in assuaging hMSC mortality under H/R-like insult has been delineated.

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Methods: The CoCl2 mimicked H/R conditions in vitro was investigated upon DOR activation, mediated via DADLE. hMSCs loss of viability, reactive oxygen species (ROS) production, inflammatory responses and disconcerted unfolded protein response (UPR) were assessed using AnnexinV/PI flow cytometry, fluorescence imaging, mitochondrial complex 1 assay, quantitative PCR, immunoblot analysis and ELISA. Results: H/R like stress induced apoptosis of hMSCs was significantly mitigated by DADLE via modulation of the apoptotic regulators (Bcl-2/Bax) along with significant curtailment of ROS and mitochondrial complex 1 activity. DADLE concomitantly repressed the misfolded protein aggregation, alongside the major UPR sensors: PERK/BiP/IRE-1α /ATF-6, evoked due to the H/R mimicked endoplasmic reticulum stress. Undermined phosphorylation of the Akt signalling pathway was observed, which concerted its effect onto regulating both the pro and antiinflammatory cytokines, actuated as a response to the H/R-like insult. The effects of DADLE were subdued by naltrindole (specific DOR antagonist) reaffirming the involvement of DOR in the process. Conclusion: Taken together these results promulgate the role of DADLE-induced DOR activation on improved hMSC survival, which signifies the plausible implications of DORactivation in cell-transplantation therapies and tissue engineering aspect.

Keywords: DADLE; transplantation

delta

opioid

peptide;

hMSCs;

hypoxia-reperfusion;

survival;

STMC-16 Design and Development of Three-Dimensional Human Neural stem cells using Natural Hydrogels Venkatesh Katari#, Pearlin Hameed, Ankita Parida, Shounak Ghosh, Dwaipayan Sen* Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology, Vellore, Tamil Nadu. Background: Neural stem cells (NSCs) are potential enough to regenerate three different types of cells of nervous system and become a promising cellular therapeutic target for treating various neurodegenerative diseases and spinal cord injuries. Therefore it is very important to maintain their structural and functional properties for further clinical applications. Methods: NSCs were derived from human mesenchymal stem cells (hMSCs) using epidermal and fibroblast growth factors (EGF & FGF) under serum-free conditions. Neurospheres were enzymatically dissociated using accutase treatment. The single-celled NSCs were characterized using specific markers such as Nestin, Sox2 and Mushashi 1 by immunofluorescence and reverse-transcriptase polymerase chain reaction (RT-PCR). The tri-differentiation capacity of NSCs was examined using specific markers such as GFAP (astrocytes), MBP (oligodendrocytes) and MAP2 (neurons). Three-dimensional cultures of NSCs were generated using collagen-based hydrogels. The viability of NSCs in the hydrogels was determined using propidium iodide. The morphological and molecular characterization of NSCs embedded in the collagen-based hydrogels was examined using immunofluorescence and RT-PCR methods. Results: Neurospheres were successfully generated from hUCB-MSCs by induction with EGF and FGF. The expression of NSC markers: Nestin, Sox2 and Musashi-1 determined by immunofluorescence and RT-PCR confirms the generation of NSCs from hMSCs. The expression of tri-lineage markers such as GFAP, MBP and MAP2 in the tri-lineage initiated NSC 225

culture confirms the presence of NSC characteristic features in the generated NSC culture. The NSCs grown in hydrogels did not adversely affect the expression of NSC markers (Nestin, Sox2 and Musashi-1) and tri- lineage markers (GFAP, MBP and MAP2) in 3D-hydrogel culture system. These results concluded that the adaptability of MSC-derived NSCs with natural hydrogels in in vitro. Conclusion: The present approach provides a simple and robust development of threedimensional NSC cultures in in vitro. Keywords: Neural Stem cells, Three-dimensional, Collagen, Mesenchymal stem cells, neurodegenerative diseases, spinal cord injuries.

OTHERS OTHR 1: Role of water storage in magnetic coreshell on MR relaxivity Palani Sharmiladevi1, Viswanathan Haribabu1, Abubacker Sulaiman Farook2, KoyeliGirigoswami1, Agnishwar Girigoswami1* 1

Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research & Education (CARE), Kelambakkam, Chennai 603 103, INDIA. 2 Department of Radiology, Chettinad Hospital & Research Institute (CHRI), Kelambakkam, Chennai 603 103, INDIA. *Author for correspondence: [email protected], Tel: +91-9445268615

Background: The fascinating ability of Magnetic Resonance (MR) Imaging in the diagnosis and staging of disease conditions has gained the attention of many researchers to develop MR probes called Contrast Agents (CAs) based on T1 and T2 relaxation properties. Well known T1 and T2 contrast agents are Gadolinium chelates and Iron oxide nanoparticles. Currently researchers are more focused in developing CAs with dual mode i.e. T1 and T2 in order to provide better diagnostic feature as compared to single mode. It is hypothesized that the efficiency of the newly developed dual mode CAs primarily depends on the interaction of the CAs with the water protons. In this study, we have fabricated magnetic nanoparticles coated with different shell thickness of silica to act as the water storage. The effect of the water storage on the MR relaxivity was investigated in order to optimize the silica shell thickness. Methods: Manganese doped iron oxide nanoparticles were prepared by chemical coprecipitation method followed by surface modification using silica with variable thicknesses. Results: Increase in silica shell thickness showed that MR Relaxivity decreased gradually reaching a minima after certain shell thickness as noted from spectrophotometric and phantom MR image analysis. This indicates that the silica shell acts as storage for water molecules and allows its interaction with the magnetic core. Conclusion:It was concluded that the enhancement of image contrast depends on the water storage in the mesoporous silica shell that interacts with the magnetic core and silica coated magnetic core can be a better contrast agent in T1 and T2 weighted MRI. Keywords:Magnetic nanoparticles, Dual mode CAs, Mesoporous silica and water storage.

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OTHR 2: Effect of diesel exhaust particles on airway epithelial model Amalu Navas, Pradeep kumar S S and Dr. A Maya Nandkumar*. Division of Microbial Technology, Bio Medical Technology Wing Sree Chitra Tirunal Institute for Medical Sciences & Technology, Poojappura, Thiruvananthapuram 695012. *Email: [email protected]

BACKGROUND: Atmospheric particulate matter (PM), an indispensable component of urban pollution, is a mixture of solid and liquid particles differing in origin, dimension and composition. Diesel exhaust particulate (DEP), which constitutes the major part of PM, is characterized by a carbonic mixture composed of approximately 18,000 different highmolecular-weight organic compounds. Diesel engines release 10 times the amount of NO2, aldehydes and breathable PM compared to unleaded gasoline engines and more than 100 times that produced by catalysed gasoline engines. These data gain significance considering the fact that diesel-powered vehicles are becoming more popular. DEP and polyaromatic hydrocarbons (PAH), once deposited on airway mucous surfaces easily pass through epithelial cell (ECs) membranes, bind themselves to cytosolic receptors and then affect cell growth and differentiation. METHODS: We investigated the biological effects of DEP-PM on the human lung EC line A549. TEM and DLS were used for characterisation of the particle which was collected by in house methods. MTT assay was done for assessing the test dose concentration of the particle on the viability of the cells. LDH was measured to evaluate the presence of tissue or cell damage. Electric Cell-substrate Impedance Sensing (ECIS) has been applied to study in vitro toxicity by detecting and quantifying morphology changes. RESULT: LDH assay results suggested an increase in dose dependent cytotoxicity and were in agreement with the MTT assay observations. ECIS (electric cell substrate impedance sensing) assay, the non-invasive biophysical approach showed a dip in impedance in the highest cytototoxicity dose as well. CONCLUSION: These results suggest a detailed cytotoxic and genotoxic assessment of the particle, for complete delineation of the molecular mechanisms activated by DEP and the depth of damage it would cause as a occupational hazard to Traffic policemen who have a history of early onset of respiratory diseases. KEYWORDS: Diesel Exhaust Particles, airway epithelium Cytotoxicity, Impedance.

OTHR 3: TREATMENT OF BIOWASTE TO A PHARMACEUTICAL EXCIPIENT Shebina P.Rasheeda*, M Shivashankarb, Sanal Dev c a

Department of Pharmaceutical Chemistry ,SAS, VIT, Vellore c Department of Pharmaceutical Chemistry, Al Shifa College of Pharmacy, Perintalmanna,Kerala

Background: The effective treatment and utilization of bio waste have been emphasized in our society for environmental and economic concerns. Recently, the eggshell waste in the poultry industry has been highlighted because of its reclamation potential. So a means of recycling egg shell gains attention. This study describes an effective treatment process to recover useful biomaterial from eggshell waste and its utilization in pharmaceutical products. Egg shells are 227

rich source of mineral salts, mainly calcium carbonate(about 94%) but the egg shells are not properly utilized and are simply discarded as a biological waste. An attempt has been made to prepare and evaluate calcium carbonate from egg shell due to its potential application in the development of a new option of pharmaceutical excipient. Method: The egg shell CaCO3 was subjected to several chemical processes and recycled into pure CaCO3.The cleaned egg subjected to purification by using two different methods one by chemical treatment and the other by heat treatment. Then it is subjected to chemical treatments. Calcium carbonate separated by both methods are subjected to monographic analysis and analytical studies for characterization Result: The purity of the prepared compound was within the pharmacopoeial limits and has good quality Conclusion: The findings indicated that calcium carbonate from egg shells can be used as an alternative pharmaceutical excipient. It is an effective method for processing bio waste in to useful pharmaceutical excipient. Key words: Egg shell, Calcium Carbonate, Bio waste, Monograph

OTHR 4: Fabrication and optimization of key components in a bioreactor and its use for oil spill remediation Ankita Das, Sagarika Sarangi, Parvaiz A. Shiekh and Ashok Kumar* Department of Biological Sciences and Bioengineering, Centre of Environmental Science and Engineering, Indian Institute of Technology Kanpur, Kanpur – 208016, UP, India * Email : [email protected]

Background: Oil Spill and its related organic compounds are a great threat to aquatic ecosystems. It is also a huge loss to the industries involved in oil extraction from sea-bed. Hence, there is a need for an efficient system to separate oils/organic pollutants from the marine ecosystem and their remediation for a safer environment. Recently, the collection of oil using hydrophobic and oleophilic materials is of considerable interest and polyurethane is such type of a polymer having excellent sorption capacity, low density, and easily scalable fabrication processes. Since polyurethane is amphipathic, it is necessary to make some hydrophobic modification. Here in our work we have planned to construct a fluidized membrane reactor for bioremediation of crude oil using microbes isolated from polymer dump site. Methods: Polyurethane cryogels were prepared by dissolving Pellethane® 2363-80AE TPU in dimethyl sulphoxide. Alongside, electro-spun mats were also made using a 2:3 mixture of N,N dimethyl formamide and tetrahydrofuran. Modification was carried out using various natural waste products and chemical methods like vapor deposition of methyltrimethoxysilane (MTMS), addition of lignin, hydrophobically modified rice husk silica with subsequent comparison with that of the electro-spun mat. Physico-chemical characterization was carried out for all the samples before and after immobilization of microbes isolated from oil-contaminated soil. Results: Fourier Transform Infrared Spectroscopy and Scanning Electron Microscopy showed successful incorporation of the modified particles on the cryogels. Hence water contact angle was highest, around 122o for the MTMS-modified cryogels followed by unmodified 228

polyurethane and electro-spun mat. Absorption kinetics resulted in a 200% (w/v) increase of crude oil absorption with the modified cryogels and around 40% (v/v) decrease in oil concentration was observed after microbial treatment. Conclusion: The modified polyurethane along with microbial immobilization was observed to be a better absorbent for crude oil and cleaning-up of spills. Keywords: Oil spill, bioremediation, polyurethane, cryogels, electro-spinning

OTHR 5: monocyte response to plantonic and biofilm growing Pseudomonas Keerthi.S, Pradeep Kumar SS, Kavita Raja, Maya Nandkumar A* Div. Of Microbial Technology, BMT WING, Sree Chitra Tirunal Institite for Medical Sciences and Technology, Thiruvananthapuram 12

Infections are caused by pathogens while chronic infections are a result of biofilm formation in tissue or implanted devices and the infection pathology differs in such cases. Monocytes/ Macrophages are phagocytes which play crucial roles in clearance of invading pathogens, but are often found to be ineffective in clearing biofilm infections. Therefore the aim of our study was to understand the interactions of monocytes with Pseudomonas both in planktonic and biofilm mode in-vitro. Biofilms formed on endotracheal tubes (ETT) and planktonic Pseudomonas were exposed to 105 monocytes (THP1). Change in morphology of monocytes was assessed using Phase contrast and fluorescence microscopy. Bacterial internalization by monocytes was evaluated by Gentamicin Protection Assay. Monocyte ROS production was quantified using DCFHDA method. Unexposed monocyte maintained their spherical morphology whereas the monocytes exposed to planktonic cells (4Hr post exposure) developed pseudopod like structures, which changed drastically by 18 hours leading to necrotic morphology. Upon AO staining blebbing was observed in infected cells. A few dead monocytes were also observed. On the contrary, monocytes adhesion on biofilm was observed only after 18hrs of exposure. A detailed examination of the morphology revealed blebbing on the monocyte with very few internalised bacteria. ROS production was the highest when monocytes were exposed to planktonic bacteria. A similar trend was observed in ROS production by monocytes biofilm on ETT material. Viable counts following gentamicin protection assay revealed an increase in internalized bacteria over a period of 4hrs when exposed to planktonic Pseudomonas. On the contrary, no significant increase was observed in viable counts over a period of 4hrs when exposed to biofilms. Keywords: Monocytes, Pseudomonas, Biofilm, Endotracheal Tube.

OTHR 6: Conversion of Biowastes into Bioceramics via Solid-State Method Choudhary R1, Sasikumar S1 1 Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore -632014, Tamil Nadu, India. Background: Scientists are attempting new methodologies to replace expensive synthetic reagents and toxic chemicals by biowastes for the preparation of different biomaterials for biomedical applications. Natural origin and low cost of biowastes have attracted researcher’s attention in converting them into valuable biomaterials.

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Methods: Wollastonite, forsterite and diopside were successfully synthesized by utilizing different wastes (eggshell and rice husk). Decomposition, alkali treatment, and acid precipitation was employed to extract silica from rice husk. Solid state route was adopted to synthesize these bioceramics. The relationship between their chemical composition and properties was studied (phase optimization, apatite deposition and dissolution behavior). Results: The phase purity of wollastonite was achieved at 1100 oC. Biomineralization assay of wollastonite reveals that hydroxycarbonate apatite (HCA) layer was deposited on its surface within three days of immersion in SBF. Wollastonite had remarkable bioactivity in SBF due to the presence of calcium in its composition. The calcination temperature optimized for forsterite was 1300 oC. Slow apatite formation was observed on the surface of forsterite after immersion in SBF for 35 days due to presence of magnesium content in the material. The chemical composition and dissolution behavior were responsible for the variation in the apatite deposition ability of forsterite. It can be suggested that the bioactivity of forsterite can be improved by substituting calcium at magnesium site. Utilization of wastes as starting materials reduced the melting point of diopside. The moderate biomineralization on the surface of diopside was found due to the existence of both calcium and magnesium as chemical constituents. Conclusion: Utilization of different biowastes can act as efficient alternatives for synthetic starting materials. The appropriate control over the compositional ratio of chemical constituents can assist in designing a suitable composite material for biomedical applications. Keywords: Bioactive silicate, Rice husk, TG-DSC, Apatite, Dissolution, Biomedical applications OTHR 7: Secure Transmission of DICOM images by comparing different cryptographic algorithms Umang Agarwal, Navamani T.M, Rohit Agrawal, Akhil Bharadwaj Vellore Institute of Technology, Vellore -632014, Tamil Nadu, India. Background: DICOM stands for Digital Imaging and Communications in Medicine. DICOM is a communications protocol as well as a file format, which means it can keep medical information, like ultrasound and MRI images, along with a patient's information combined in one file. DICOM images should be transferred securely from one host to another host and the one possible way to receive the file also to be in the same DICOM format. Hence, the aim of this work is to implement the concept of secured transmission of DICOM images by preserving the properties such as Confidentiality and Integrity using Cryptographic Algorithms. Methods: This work uses two types of cryptographic algorithms namely AES (Advanced Encryption Standard) and Blake Hash Function to preserve the security requirements such as confidentiality and integrity of DICOM images. The initial concern of the system lies in providing confidentiality in the communication of the images which can be done using AES algorithm. The integrity can be provided using Blake Hash Function. Results: In this work, comparison analysis of AES with other cryptographic algorithms like Triple DES, IDEA, Blowfish, and SEED algorithm is done. The comparison was done with respect to their execution time where the input was the DICOM image. The analysis showed that AES outperforms all other algorithms with its performance. Conclusion: DICOM images are more widely used in the field of medical science as they are practiced in radiology, cardiology, pathology, dentistry, ophthalmology and used to store, handle 230

and send various images over the network obtained in X-ray, CT, MRI etc. Hence, preserving the security of DICOM files plays crucial role which has been successfully implemented in this work. Keywords: DICOM images, Confidentiality, Integrity, Cryptography, Advanced Encryption Standard (AES), Blake Hash Function

OTHR 8: Design and optimization of key components of a fixed bed bioreactor for clearing oil spills Sagarika Sarangi, Parvaiz Ahmad Shiekh, Ashok Kumar* [email protected]* Department of Biological Sciences and Bioengineering, Centre of Environment Science and Engineering, Indian Institute of Technology Kanpur, Kanpur – 208016, U.P- India Background: In recent years, oil spills has led to severe environmental hazards. Chemicals present in crude oil are a threat to the ecosystem. Moreover, potential toxicity of degraded products on marine ecosystem limits the efficacy of bioremediation. Hence in our study, we have designed and optimized a system that can successfully uptake and degrade crude oil without releasing potential xenobiotics to the environment. Polyurethane cryogels with macro porous structure were used as base material. Their properties were enhanced using various natural materials, making them biocompatible. Silica micro particles were used to immobilize isolated microbes to enhance their efficiency. Methods:Polyurethane cryogels were prepared by dissolving Pellethane® 2363-80AE TPU in DMSO (Dimethyl Sulfoxide). Various natural wastes like lignin, hydrophobically modified rice husk ash were added to polyurethane cryogels. Physicochemical properties like strength, porosity and surface morphology were checked through Scanning Electron Microscopy (SEM), tensile strength and swelling kinetics. The performance of the cryogel with all the modifications were evaluated. Microbes from contaminated soils were isolated, characterized and immobilized to enhance their efficacy. We assembled the components of a reactor and assessed its working, simultaneously checking its effect on environment. Results: Polyurethane (5%) can swell up to 500 times its volume. It had a rupture point at a stress of 650 MPa. There was 100 to 150 % rise in total absorption by cryogels incorporated with lignin and silica. There was a 40 - 50 % degradation of crude oil by free microbes was observed. Through various assays it was seen that microbes were viable and metabolically active inside the micro particles. Conclusion: Polyurethane (5%) was mechanically strong and had good porosity to be used as a material for crude oil absorption. Microbes were metabolically active and functioning within the micro particles. Hence the whole system can work for oil spill cleanup. Keywords: SEM, cryogel, tensile strength, micro particles, immobilization

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OTHR 9: Nonlinear optical and optical limiting properties of Trisodium citrate pentahydrate with femtosecond laser pulse excitation N. Suneethaa, D. Rajan Babu* Advanced Materials Research Centre, School of Advanced Sciences, Vellore Institute of Technology, Vellore, India 632014 E-mail: [email protected], [email protected] Lasers have been used in various fields for many purposes, and the advancement of laser science was greatly enhanced with the advent of nonlinear optics. The usage of the laser has many advantages as well as negative effects on human life. From the investigation of laser-tissue interaction, it was found that among the human organs, eyes will be easily injured by laser irradiation. In order to overcome this problem with the use of a laser, optical limiters can be used to protect human eyes. Optical limiting is the third order nonlinear phenomena which can be used to block high-intensity light before reaching the retina. So, in the direction of protecting human eyes and optical sensors, in the present investigation we are reporting the optical limiting properties of Trisodium citrate pentahydrate (TSCP)single crystal. Third-order nonlinear optical properties of TSCP were studied by using the Z-Scan technique with the excitation of femtosecond laser pulses at 794 nm. From third-order nonlinear optical studies, we have found that the material exhibiting self-focusing effect and also three photon absorption (3PA) is taking place due to strong reverse saturable absorption (RSA) nature, which emphasizes the essential requirement for passive optical limiting application. Optical limiters constructed based on RSA phenomena are useful for the passive optical limiting application. References [1] Benjamin A. Rockwell, William P. Roach,Mark E. Rogers, IEEE 94 (1994) 185-187 [2] L.W. Tutt, T.F. Boggess, Prog. Quantum Electron. 17 (1993) 299–338.

OTHR 10: Nailbed Image Acquisition and Feature Extraction Haran Kumar S ,Vinoda. P , Deepali Chaudhari, J. B. Jeeva Dept. of Biomedical Engineering, Vellore Institute of Technology Vellore – 632014 Background: Nailbed capillaroscopy is a non-invasive method of the examination of vascular variations in the nailbed exhibited due to different systemic connective tissue sicknesses, as in systemic sclerosis, it is helpful in the diagnosis of cardiovascular anomalies, such as Reynolds’s phenomena. The instrument used at present is expensive and an alternate design is presented which can be made at an affordable cost. Methods: The proposed instrument consists of a light source, microscope with 100X magnification and a digital camera connected to the microscope. The light source consists 3 sets of white LEDs, arranged 120 degrees apart in a circular pattern around the objective lens. To view the micro-capillaries on the computer, the nailbed of a healthy volunteer is illuminated and captured by the instrument. Results:. The features of the capillaries such as apex width, capillary density, capillary width, capillary length, arterial and venous limb diameter, internal and loop diameter were measured. Table shows the measured values of the capillary features of the volunteer. Conclusion: From the results obtained it can be concluded that the method followed gives comparable output on par with the commercial product. The same instrument could be miniaturized and be made available at an affordable for all hospitals. 232

Keywords: Nailbed capilloroscopy, Microcapillaries, Systemic sclerosis, Cardiovascular anomalies OTHR 11: Development of Near-Infrared Diffuse Optical Tomographic Imager and Phantom study Sathya.A, Deepan.T, Jeeva JB School of Electronics Engineering, VIT, Vellore, India Background: Diffuse optical tomography (DOT) is an emerging medical imaging modality in which tissue is illuminated by near-infrared light sources. The multiple scattered light which emerges is collected by an array of detectors. The propagation physics is used to infer the localized optical properties of the illuminated tissue. The most important applications of DOT are detecting tumors in the breast and imaging the brain, and detection of abnormalities in fingers. In this work a Near infrared-DOT system was developed and potential imaging quality was studied Methodology:The developed NIR-DOT system consists of a laser diode source (830nm,180mW) converted into fan-beam using plano-concave lens, array of photodiode detectors, mechanical arrangement for circular and up-down movement, data acquisition card and computer. Parrafin wax phantoms with embedded abnormality is placed between the source and the detector. 200 projections were obtained for one rotation and fed to the computer through the data acquisition card. Results: The acquired data was reconstructed using filtered back projection algorithm and crosssectional image of the phantom was obtained. The diameter of the phantom was 8cm. This shows that the device can be used to obtain cross-sectional image of human soft tissues of comparable size. Conclusion From the phantom studies it can be concluded that the Near Infrared Diffuse Optical Tomographic Imager developed can be validated using biological tissue phantoms and then used for human studies. This has great potential as an inexpensive non-invasive diagnostic tool in future for medical imaging of human soft tissues. However the reconstructed image has lot of artefacts which degrades the quality. This can be reduced by using appropriate filters. Improved hardware design can be implemented to enhance the quality of the acquired data. Keywords: Diffuse Optical Tomography, near infra red imager, Phantom, Fan beam OTHR 12: Calibration of Computed Tomography Number using cheese phantom with different kVp tube ratings S. Sriram Prasath1, B. Arun1, Moses Arun Singh1, Melinda1, VidhyaShree1. K.Senthilnathan2,N.Arunai Nambi Raj3 , P.Ramesh Babu2 1. Tata Medical Center, Kolkata, India. 2. Department of Physics, School of Advanced Sciences,VIT, Vellore, India. 3. Centre for Biomaterials, Cellular and Molecular Theranostics, VIT, Vellore Background: CT numbers in Hounsfield units (HU) are used to correct for tissue inhomogeneities in radiotherapy treatment planning systems (TPS). In order to guarantee a precise treatment, it is important to obtain the relationship between CT HU and electron densities in 233

radiotherapy, which is the basic input for radiotherapy planning systems which consider tissue heterogeneities. Methods: A biological tissue Gammex RMI Tissue Characterization Phantom (TCP) was used in conjunction with a CT scanner to plot the relationship between electron density (ρe) and their corresponding CT number in HU for 80,100,120 and 140 kVp tube ratings respectively. The Gammex RMI TCP is a 33 cm diameter solid water disk with a matrix of sixteen 2.8 cm diameter holes which holds interchangeable rods of various tissue and water substitutes of known ρe. Separate graphs were plotted for relative ρe against the corresponding HU for each kVp tube rating. Results: The graphs showed similar correlation between relative ρe and HU in the range between -1000 and +1000 HU regardless of the kVp tube rating. However, there is an appreciable discrepancy in the correlation between ρe and HU in the ranges above +1000HU for different kVp settings. This might influence the accuracy of the dose calculation in the TPS in patients with metal implants and using kVp specific image value density tables (IVDT) might reduce this inaccuracy. Whereas, in patients with no implants where the HU is ranging between 1000 to +1000, it may not be necessary to use kVp specific IVDT curves. Conclusion: There is appreciable discrepancy in pe and HU correlation in the non-biological HU ranges above +HU. Using kVp tube rating specific IVDT may increase the accuracy of dose calculations in patients who are getting treatment in areas around or beyond metal implants. OTHR 13: Cranio spinal irradiation techniques: a dosimetric comparision of helical tomotherapy with volumetric modulated arc therapy Sathiya Seelan M1, 2, Murali R2, Tamilselvan K1, Padma G1, Arunai Nambi Raj N3 1.Department of Oncology, Apollo Cancer Hospital, Jubilee Hills, Hyderabad 2.Department of Physics, School of Advanced Sciences ,VIT , Vellore 3. Centre for Biomaterials, Cellular and Molecular Theranostics, VIT, Vellore Background:CranioSpinal Irradiation (CSI) is a challenging procedure of treating various central nervous system malignancies. Large PTV size (includes whole Brain and Spinal Cord) requires field matching due to technical limitations of conventional linear accelerators (LA).Helical Tomotherapy (HT) could help to avoid these limitations as irradiation of long fields ispossible without field matching. This study aims to assess the dosimetric comparision of treatment plans between HT and Volumetric Modulated Arc therapy (VMAT) of CSI. Methods: CT datasets of Four (n=4) previously treated patients with medulloblastoma were used to generate VMAT plans in Eclipse and HT plans inVoLO planning system. In both plans total dose of 36Gy in 20 fractions with 1.8Gy per fraction was prescribed such that at least 98% of the volume of PTV received at least 95% of the prescription dose. Plans were compared using PTVs conformation number (CN), homogeneity index (HI), normal tissue mean dose statistics, body mean dose and treatment time. Results: HT plans showed better homogeneous dose distribution to PTV with a mean (SD) HI of 0.06 (0.006) vs. 0.10 (0.019) for VMAT plans. VMAT plans showed marginally better CN mean of 0.83 vs. 0.76 for HT plans. Normal tissue mean dose statistics showed 1.1-1.39 (mean, 1.25) times higher in VMAT plans compared to HT plans. Both the plans showed average body doses were same.HT plans showed mean beam on time 11mins vs. 6.25mins for VMAT plans.

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Conclusion: The results are suggesting HT is technically easier for treating CSI without any junction matching and dosimetrically superior than VMAT. Keywords: CSI; Helical Tomotherapy; VMAT OTHR 14: Gravity of point of care testing (poct) IN ICU, OP, ED and its baleful effects on misapplication. Nivedha Subramanian Rajalakshmi engineering college Point of care testing (PoCT) or bedside testing is defined as medical diagnostic testing –the goal is to collect the specimen and obtain the results in a very short period of time for providing the best treatment plan for the patients. This contrasts with the conventional way of medical laboratory testing, which entailed sending off specimens away from the point of care and then waiting for hours for obtaining the results during which time care would continue without desired information in the case of emergency. Point- of-care tests includes blood glucose, blood gas, urine strips testing, pregnancy testing, cholesterol screening etc. The importance of using PoCT in ICU(Intensive Care Unit), OT(Operation Theatre), ED(Emergency Department) and the adverse effects caused due to ignorance of various regulatory parameters are being focused in the research work. This research article gives an all-inclusive study of PoCT and its regulations in hospitals. OTHR 15: Image analysis of daily set up in radiotherapy using On Board Imager and Electronic Portal Imaging Device Siva Kumar S1,2, Minu Boban1, Venkatesan1, Jomon Raphael1, Mathew Varghese1, R.Murali2, N.Arunai Nambi Raj3 Department of Radiation Oncology, Amala Institute of Medical Sciences, Thrissur1, Department of Physics, SAS, VIT University, Vellore2, Centre for Biomaterials, cellular and Molucular Theranostics, VIT University, Vellore3 Background: Radiotherapy is one of the major modality of treatment in cancer patients. Modern linear accelerators are available to deliver high end treatment precisely targeting cancer cells. Imaging plays a major role in achieving the precise treatment delivery. The aim of this study was to analyse the online and offline images for set up accuracy. The study includes Pre treatment Imaging time and treatment time for our radiotherapy patients. Methods: Fifty patients were chosen for the study with various sites for treatment. A baseline study was done for two dimensional Brain palliative radiotherapy patients. Patients were explained the simulation and treatment procedures prior to set up and treatment. Individual immobilizing devices and materials were prepared using ORFIT® thermoplastics. Pre-treatment online imaging was done using on board imager and electronic portal imaging device attached to the linear accelerator. Bony landmarks were chosen for analysis in this study. Results: All the fifty patient’s online images were analysed using Aria workstation retrospectively. The bony landmarks had shown a good matching for brain RT patients. Small variation was seen in the head and neck radiotherapy patients at the C1 and C6 vertebrae. Significant variation was seen in arm position for breast cancer patients. Pelvic site set up images shown a significant variation at the first alignment of patient. Conclusion: The analysis of online images showed set up accuracies and variations in four dimensional axis. This helped to define the treatment margins for different treatment sites and 235

thus to reduce the normal tissue damage. The analysis of imaging time helped to reduce the patient waiting time in our department for treatment. OTHR 16: A study of inhalation drugs in the management of Asthma in northern parts of Tamilnadu MurugeshShivashankar* and Mani Dhandayuthapani. Pharmaceutical Chemistry Division, School of Advanced Sciences, VIT University, Vellore, Tamil Nadu, India BACKGROUND: Asthma, a major problem facing our polluted environment. It is a reversible and curable disease. Symptoms of asthma include episodic breathlessness or dyspnoea (especially at night or early morning, or after some exertion),Wheezing,Cough (especially at night or early morning, or after some exertion) and Chest tightnessTreatment of asthma includes the class of drugs from beta-blockers (Salbutamol, formoterol) and inhaled corticosteroids(beclomethasone,budesonide).Majorly compared with the oral and I.V’s, Inhalation route has more efficacy. AIM: A detailed study of inhalation drugs and its socio-economic benefits to asthma in the northern parts of Tamilnadu. METHODS: A multicentric, noncomparative,random sampling study was conducted in five hundred asthmatic patients in northern parts of Tamilnadu (includes Chennai and suburb districts).Based on treatment patients were grouped as inhalation and oral medication. Inhalation shows more efficacy in the treatment of asthma.currently, inhalation drugs are classified into relievers and controllers.The drugs are administered through inhalation route via.Dry powder inhaler and Pressurised metered dose inhaler. RESULTS: A total of 500 asthmatic patients were surveyed in this study.the inhalation drugs were administered through inhalers like DPI,pMDI,andNebulizers with 47%, 50% and 3% patients adhered respectively.a combination of Reliever and controller (ICS+LABA) shows significant control in asthma management than other monotherapy. Two hundred seventy-one asthma patients experienced less exacerbation after medication. CONCLUSION: In asthma management, All the inhalation drugs in combination show better control and superior patients response in the management of asthma with more impact on economic aspects of the patients.

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OTHR 17: Exploring genetic targets of psoriasis using genome wide association studies (GWAS) for drug repurposing Harshit Nanda, Nirmaladevi Ponnusamy, Sajitha Lulu, Arumugam Mohanapriya* Department of Biotechnology, Vellore institute of technology (VIT) university, Vellore, Tamil Nadu, 632014 * Email: [email protected] Background: Psoriasis is a chronic inflammatory disease causing itching in the body and pain in the joints. Currently, no cure is available at a commercial level for this disease. Genome wide association studies (GWAS) provide a data that help in better understanding of this disease and further possible permanent cure of this disease. Objective: The major goal of present study is to identify potent genetic targets of psoriasis disease using GWAS. Methods: GWAS catalogue, GeneAnalytics, canSAR protein annotation tool, Var elect, Drug bank, Proteonomics database, ProTox software. Results: By exploring GWAS catalogue, 126 Psoriasis associated genes were identified. Two genes Interleukin 13 (IL13) and POLI are Food and Drug Administration (FDA) approved targets. The superpathways obtained from GeneAnalytics software resulted in involvement of these genes in the immune system, Jak/Stat pathway, Th17 and Wnt pathways. Conclusion: Psoriasis is generally accompanied by depression and environmental stresses in patients. 68 compounds having drug action were obtained from canSAR protein annotation tool. Localisation results depict that maximum genes are present in cytoplasmic cellular components. FDA approved drugs provide a method for permanent cure of Psoriasis. Keywords GWAS, Psoriasis, ontology, mutations, disorders, expression, drug action.

OTHR 23: Sensitivity Analysis of Dual Steering-Wheel Micro Structured Gas Sensor in Terahertz Wave Band Ramachandran A1, Ramesb Babu P2, Senthilnathan K2. 1. School of Electronics Engineering, VIT University, Vellore, Tamil Nadu, India. 2. Department of Physics, School of Advance Sciences, VIT University, Vellore, Tamil Nadu, India. BACKGROUND: The main objective of this work is to design a novel photonic crystal fiber (PCF) sensor which finds diverse applications. METHODS: We design a dual steering-wheel micro structured photonic crystal fiber (DSWMOF) based on evanescent-field sensing by introducing a large noncircular steering-wheel like structure in the cladding region. RESULTS: Further, using the finite element method, we compute the relative sensitivities of biosensor to be 97% and 92% for the incident frequencies of 0.5 and 1THz, respectively. We find that the confinement loss is related to the structural parameters of the sensor. Using this fact, we have been able to design a biosensor with the desired quality of low loss and high sensitivity. CONCLUSION: The proposed sensor may be useful for the detection of any kind of chemical and biological gases. KEYWORDS: Photonic crystal fiber, evanescent-field, steering-wheel like structure, finite element method.

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OTHR 25: Device to prevent alopecia for cancer patients Renu Deepthi and S. Muthulakshmi Department of Biomedical Engineering, Dhanalakshmi Srinivasan Institute Of Technology.Trichy. [email protected]

The most stressful side-effects of chemotherapy is alopecia. Even though the alopecia is not lifethreatening, it affects the patients’ psychologically and due to this some patients are rejecting the curative treatment. A new scalp cooling system is designed and the aim of this study is to assess the efficacy of this to the patients receiving various chemotherapeutic regimes that are frequently associated with significant alopecia. A helmet is used in which the peltier thermoelectric module is adjusted to the desired temperature, therefore heat is scavenged from the patients scalp, and along with that a temperature sensor, heart rate sensor and an accelerometer are placed for continuous monitoring of the patients. The scalp cooling helmet is maintained at a temperature below 15˚C before, during and after chemotherapy. A reduction in tissue metabolism as a response to the hypothermia could simply make hair follicles less susceptible to drug damage with scalp cooling. The system is well tolerated and is a very effective method for protection from hair loss caused by chemotherapy. Our results are comparable with and, in most cases, better than those reported in other studies using various alopecia preventive methods. Key words: Alopecia, Chemotherapy-induced hair loss, Scalp cooling, Hair preservation, Hypothermia.

OTHR 26-- Eco-friendly and cost-effective Pd NPs using Lagenaria siceraria: Larvicidal activity on mosquito vectors and toxicity study against Zebra fish and HeLa cell lines V.N. Kalpana, V. Devi Rajeswari* Department of Biomedical sciences, School of Biosciences and Technology, VIT, Vellore –632 014, Tamil Nadu, India E-mail: [email protected] Mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. The use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Therefore, a safe, nontoxic and costeffective drug is urgently required to eliminate this problem from every corner of the world. Today, nanotechnology is a promising research domain which has a wide range of application in vector control programmes. These are nontoxic, easily available at affordable prices, biodegradable, and show broad-spectrum target-specific activities against different species of vector mosquitoes. This research proposed a novel method of green synthesis of Palladium nanoparticles (Pd NPs) using Lagenaria siceraria, which acts as reducing and capping agent. The green synthesized Pd NPs were characterized and tested against fourth instar larvae of Anopheles stephensi. The maximum efficacy was found to be 1.94 μg/mL in synthesized Pd NPs against the larvae of A. stephensi when compared to Lagenaria siceraria. No mortality was observed in the control. The results recorded from FTIR, XRD, SEM, and TEM analysis support the biosynthesis and characterization of Pd NPs. Furthermore, toxicity studies of Pd NPs were evaluated against zebra fish Danio rerio using direct exposure to Pd NPs. In vitro cytotoxicity evaluation of Pd NPs was carried out using HeLa cells by MTT cell viability assay. Overall, our results highlighted out that L. siceraria synthesized Pd NPs may be employed to develop newer and safer agents for vector control. Keywords: Larvicidal, toxicity, palladium, UV, SEM, HeLa cell line 238

OTHR-27: Effect of solvent coordination on the structure of β-diketo based vanadyl complexes and assessment of in-vitro antidiabetic and cytotoxicity studies ChinnaPullaih C and Sheela A* Department of Chemistry, School of Advanced Sciences,Vellore Institute of Technology, Vellore - 632014, Tamil Nadu, India. BACKGROUND: The physicochemical properties, thereby the biological efficacy, of metal complexes are affected based on their structure and geometry that varies in the presence of coordinating and non-coordinating solvents. Towards this, in the present work, we have synthesized three, hitherto unreported, β-diketone based oxovanadium(IV) complexes, say, [VO(tfdmh)2], tfdmh= 1,1,1 tri fluro5,5-dimethyl 2,4- hexane dione (Complex I), [VO(dmh)2], dmh= 2,2 dimethyl-3,5 hexane dione(Complex II) and [VO(dbm)2], dbm=1,3-diphenyl propane1,3-dione (Complex III). METHODS: The synthesized compounds are characterized by EPR, UV-Visible, FTIR, ESIMS and single crystal XRD techniques. The structural changes in the presence of DCM, DMSO and DMF were analyzed by spectroscopic techniques. Further, in-vitro glucose uptake efficacy and cytotoxicity are assessed using C2C12 (Rat, skeletal muscle) and HeLa(Human cervical cancer) cell lines respectively. RESULTS: The five coordinated distorted square pyramidal geometry is observed in DCM, a non-coordinating solvent. This transforms to six coordinated distorted octahedral geometry in coordinating DMF and DMSO as confirmed by single crystal X-ray analysis. Antidiabetic activity of the complexes are assessed by in vitro study using C2C12 cell line and glucose uptaking ability of the complexes are found to be comparable to that of the standard drug, Rosiglitazone. The uptake ability follows the order [VO(dbm)2]>[VO(tfdmh)2]>[VO(dmh)2]. In-vitro study based on HeLa cell lines were done along with cisplatin and [VO(acac)2]. The complexes show better activity than [VO(acac)2] and IC50values follow the order cis-platin>[VO(dbm)2]>[VO(tfdmh)2]>[VO(dmh)2]>[VO(acac)2]. CONCLUSION: The complex [VO(dbm)2] shows better percentage of glucose uptaking ability on C2C12 cell line and also show higher % cytotoxicity on HeLa cell line. KEYWORDS: Solvent coordination, diketone based vanadyl complexes, in-vitro antidiabetic, cytotoxicity

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Aadinath W Aarti R. Deshmukh Aarushi Sharma Aathira Pradeep Abhay Pandit Abhijit Vyas Abhishek Sinha Adarsh R K Aditya A.l.G.N. Ahmaduddin Khan Ajithkumar V Akash Gulyani Akshatha Nagaraja Aleena Mary Cherian Alina Sionkowska Amalu Navas Amit Bandyopadhyay Amit Jaiswal Amit Shukla Amrita Natarajan Amy Sarah Benjamin APRosifini Alves Claro Andrew Ebenazer Anjana J Ankita Das Ankita Das Ankush Dewle Annie John Anshu Dubey Anurup Mukhopadhyay Archana.R Archini Paruthi Arumaikkannu Arun Kota Arun Kumar Asheesh Gupta Asheesh Maheshwari Ashok Kumar Ashutosh Chilkoti Ashutosh Kumar Dubey Ashwini K Patil Asif Ali Asish Kumar Panda

134 78 96 162 1 154 194 108 176 198 170 29 119 120 21 227 2 65 93 98 212 22 199 68 224 228 130 36 71 168 173 77 38 7 69 88 100 56 24 62 124 139 85

Aswathy M.R Ayan Chatterjee Bala Vaidhyanathan Balakumar S Balashanmugam P Ballamurugan A. M. Bappa Maiti Bavya M C Bhuvanesh Gupta Bikramjit Basu Biolmed Innovations Brindha J Bupesh Raja V.K Catherine Martin Chaithanya Vinay Chetna Verma Chinchu K Sabu Chinnapullaih C Chitra S D. Sangeetha Thanaraj Debabrata Nayak Debjani Ghosh Debrupa Lahiri Deepthy Menon Delma Dcruz Devlina Ghosh Dhanaja Pillai Dhivyaa Anandan Dhivyaa Anandan Dhulika Ravinuthala Diego Mantovani Duraimurugan M Durga Lakshmi D Elakkiya Venugopal Emmanuel Joseph Emon Barua Erfath Thanjeem Erfath Thanjeem Eva C Das Falguni Pati Francesca Boccafoschi Franck Clement Gabriele Candiani

186 193 16 50 179 51 84 189 4 14 138 112 78 106 170 109 107 239 155 209 165 179 60 53 82 111 196 114 116 86 6 221 67 136 117 74 215 215 104 64 47 12 28

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Author Gandhi C Gayathri P P Gayathri Sundar Geeta Kumari Geetha Manivasagam Gobi Saravanan Kaliaraj Gopal Agarwal Gopal C Kundu Gopinath Perumal Gopinath Perumal Grace Felciya S J Gururaj Parande Gururaj Parande Harish Kumar Madhyastha Harshit Nanda Hemalatha Kanniyappan Henry Prakash M Ifra Mirza

Page no 177 193 200 137 20 122 127 24 68 121 141 72 72 18

Author Keerthi S Ketul C Popat Krishanu Ghosal Krishna Kumari Swain Krishnapriya M Kundu SC Kuthe A. M. Lakshmi S Nair Lekshmi Krishna Lickmichand M Louise M. Jennings Luis Augsto Rocha Madhubanti Mullick Madhubanti Mullick

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237 202 19 188

149 115 21 118

Indira Priyadarsini Jaivignesh M Jay Panji, Balaji Ramachandran Jaya Thilakan Jayachandran K Jayakumar R Jean Jacques Pireaux Jeeva J B Jeeva J B Jeevna Rajeev Jesslyn Kim Ean Tan Jijo Wilson J Jissy Joseph J Ricardo Muniz Ferreira Joseph Jose Josna Joseph Joyce Nirmala M Juhi Chakraborty Juna Konikkara Kalaiarasan S. Kalpana V.N. Kalyani E Kanagaraj S Kapender Phogat Kaushik Chatterjee

32 100 122

Mala R. Mamatha M Pillai Manasseh Nithyananth Manohara Dhulappa Jalageri Manoj Gupta Maurizio Vedani Mayank Bhushan

180 42 52 39 232 233 83 124 222 141 46 192 185 205 91 150 197 238 129 19 164 13

Melwin G Michael Pillay Michael Tatoulian Moghal Erfath Thanjeem Mohan Babu Ms. Amisha Desai Murugesh Shivashankar Nalinee Kanth Kadiyala Narmadha Rajeshwaran Navamani T M Neeladrisingha Das Neelima Thottappillil Neelima Varshney Neetu Singh Nicholas J Dunne Nishad K V Nivedha S Nivedha Subramanian Palani Sharmiladevi Pasupuleti S Kumar Pathmanapan Srinivetha Payel Deb

176 54 8 216 158 66 236 178 133 230 207 126 105 27 3 153 175 235 226 84 129 156

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Pearlin Hameed Pearlin Hameed Piyali Das Piyush Gupta POOJA R Poonam R Prasad Yarlagadda Prashanth R Kubasad Prasoon Kumar Pratik Das Preethi Jenifer Privita Edwina Ra G Priya G. Priyadarshani C Priyank Bhutiya Priyanka Sarkar Purva Gupta Pushpalatha C R Shiji Radhakrishnan Vidya Radhika Poojari Radhika Poojari Raelene Cowie Rahul V G Rajalekshmy Rajan Choudhary Rajavenkatesh K Rajkumar S Ramachandran A Ramanathan Yegappan Ramesh Raghavendra Ramji Kalidoss Ramya Kannan Rasmi Ranjan Behera Raul Rosalez Ibanez Raunak Kumar Das Ravindra Badhe Rebu Sundar Reetoja Nag Renji Rajendran Renju Radhakrishnan Renu Deepthi Rima Saha

125 220 70 190 149 113 33 173 87 212 146 166 116 75 208 219 108 73 80 111 81 205 44 98 147 229 184 177 237 159 15 175 202 82 17 183 159 132 182 151 191 238 187

Ripon Sarkar Rituparna Saha Roberto D Fanganiello Rodrigo S. Vieira Rohidas Arote Rohit Goyal Rohit Goyal Roopesh Pai Rutusmita Mishra Sagar Nilawar Sagarika Sarangi Sakthivel Sankaran Sampath Kumar TS Sanjay Singh Santhosh Kacham Santosh Kumar B Y Sapna Rangarajan Saranya Sugumar Sarlasrita Mohanty Sarma P. V. G. K. Sathiya Seelan Satish Jaiswal Saurabh Gupta Selvakumar Gopika Selvakumar R Sharathkumar Reddy V Shazia Shaikh Shebina P.Rasheed Shilpa Ajit Shiny John Shirley Motaung Shiva Muthuswamy Siripireddy Balaji Siva Kumar S Sivapriya Kirubakaran Sivashanmugam A Sivasubramanian M Smrutirekha Mishra Sneha Gupta Socrates Radhakrishnan Somnath Maji Sonia Kapoor Sooraj Hussian Nandyala

182 161 40 41 63 75 172 89 142 132 231 171 23 61 217 163 210 150 64 48 324 153 63 102 37 114 119 227 97 148 59 160 211 235 5 90 181 188 143 103 128 61 49

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Souvik Ghosh Sridhar TM Srimanta Barui Sriram Prasath Sriya Yeleswarapu Sruthi Alex Subathra Radhakrishnan Subhadip Basu Suchandra Bagchi Sudipto Datta Sumit Das Lala Suneetha Nagaradona Suruchi Poddar Susmita Bose Swati Sharma T V V L N Rao Tarun Agarwal Thanusu Parandhaman Tharun Selvam Mahendran Thomas J Webster Uday Roopavath Udhaya Kumar Usha Rani Thirunavukkarasu Valarmathi N Varun Saxena Varun Sharma Veena Koul Venkatesan Jayachandran Venkatesan K Venkatesh Katari Venkatesh Vakucherla Vidhya G Vidushi Sharma Vijay Kumar N. Vijayalakshmi Venkatesan Vimal Rohan K Vimala Devi Mohan Vineeth C A Vinita Dhaware Vinod Kumar Reddy Viswanathan Haribabui Vothani Sarath Yashodhan Ektare

110 57 94 233 91 208 218 86 223 96 156 232 104 13 76 152 136 201 203

Yasuharu Ohgoe Yogesh Verma Yubraj Lamichhane

10 88 123

31 92 135 171 140 206 173 25 144 213 225 99 157 95 139 30 167 147 131 165 221 79 195 144 243