(Jacob et al., 1963) postulated that the bacterial cell surface may contribute to the ..... The medium routinely used was M9 minimal (Miller, 1972), containing 0.2%.
The EMBO Journal vol.5 no.4 pp.787-791, 1986
Early replicative intermediates of Eschergihia coli chromosome isolated from a membrane complex
M.Yoshimotol, H.Kambe-Honjoh2, K.Nagai and G.Tamura3 Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo 113 Japan 'Present address: Research Laboratories of Taisho Pharmaceutical Co., Ltd., Omiya, Saitama 330, Japan 2Present address: Nippon Roche Research Center, Kamakura, Kanagawa 247, Japan 3Present address: Department of Applied Biological Science, Science University of Tokyo, Noda, Chiba 278, Japan
Communicated by F.Jacob
The replication origin region of the Escherichia coli chromosome was isolated from an outer membrane fraction. The chromosome was further purified by centrifugation in a cesium chloride gradient. Early replicative intermediates were enriched in the preparation when cytosine-l-fl-arabinofuranoside was added to the culture at the time of initiation of chromosome replication. DNA fragments with an eye structure having two branches of
