Effect of Strontium Enhanced Calcium Phosphate Coating on In Vitro Behavior of Human Mesenchymal Stem Cell (hMSC) Samuel C. Uzoechi1, a, *, Goddy C. Okoye1, b, Kennedy O. Ejeta1, c, Benjamin I. Nkem 2, d, Gideon I. Ndubuka1, e Authors Affiliation 1
Department of Biomedical Technology, Federal University of Technology, Owerri, Nigeria Medical Research Unit, Federal Medical Center, Owerri, Nigeria Email:
[email protected],
[email protected],
[email protected],
[email protected],
[email protected] 2
*
Correspondent: Ir. S.C. Uzoechi, Department of Biomedical Technology, Federal University of Technology, Owerri. PMB 1526, Owerri, Nigeria Email:
[email protected]. Tel: +234810-723-1152
1
Abstract Calcium phosphate is a widely used material as coating for metallic implants. This research describes a biomimetic coating technique based on deposition of calcium phosphate films on a Ti6Al4V plates that was used to study the effect of strontium additive on the behavior of hMSCs. Strontium additive was homogenously deposited onto calcium phosphate films on a Ti6AlV plates by using a biomimetic techniques. Strontium affected composition and morphology of calcium phosphate deposited on a Ti6Al4V plates to a varying degree according to concentration of solutions used. The effect of strontium additive on proliferation and differentiation of hMSCs depended on the solution and concentration tested. In general, all individual three coatings showed decreased hMSCs proliferation. Strontium additive demonstrated a significant increase in differentiation into osteogenic lineage when compared with the control and calcium phosphate films without strontium additive. However, no cytotoxic effect of strontium additive in the concentrations tested was detected. The Fourier Transform Infrared (FT-IR) spectra showed that this new coating closely resembles bone mineral. The techniques illustrated in this study mimics bone mineral containing strontium additive, making it constructive for studying basic processes of in vitro bone formation. The results showed in this study can be used for changing bone graft substitutes by addition of strontium additive on implants in order to affect their performance in bone repair and regeneration. Also, the system can aid rapid bone formation around the implant, reducing therewith the patient’s recovery time after surgery. Keywords: Strontium, hydroxyapatite, octacalcium phostphate, coatings, osteogenic
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Introduction Plasma-spray coatings techniques, mainly hydroxyapatite (HA) on titanium alloy (Ti6Al4V) implants have been extensively used in orthopedic surgery to reconstruct hip and knee joints. Previous studies have shown that these coatings can effectively improve clinical success to a
