Effects of a Novel p.A41P Mutation in the Swine

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Furthermore, dominant effects significantly influenced total muscle fiber number ... A41P mutation was genotyped on 429 Yorkshire pigs by the RFLP analysis ...

Korean Journal for Food Science of Animal Resources Korean J. Food Sci. An. 2018 August 38(4):711~717 DOI https://doi.org/10.5851/kosfa.2018.e9

ARTICLE

pISSN : 1225-8563

eISSN : 2234-246X www.kosfa.or.kr

Effects of a Novel p.A41P Mutation in the Swine Myogenic factor 5 (MYF5) Gene on Protein Stabilizing, Muscle Fiber Characteristics and Meat Quality Youn-Chul Ryu1,†, Eun-A Lee2,†, Han-Ha Chai3,†, Jong-Eun Park3, and Jun-Mo Kim4,* 1Division

Received

June 8, 2018

Revised

June 20, 2018

Accepted

June 25, 2018

*Corresponding author : Jun-Mo Kim Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea Tel: +82-31-670-3263 Fax: +82-31-675-3108 E-mail: [email protected] † These authors contributed equally to this study.

of Biotechnology, Sustainable Agriculture Research Institute, Jeju National University, Jeju 63243, Korea 2Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea 3Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea 4Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea

Abstract Myogenic factor 5 (MYF5) plays an important role in regulating skeletal muscle fiber characteristics, consequently affecting meat production and quality. We identified a novel p.A41P mutation in exon1 of the porcine MYF5 gene by direct sequencing. The mutation was predicted to be destabilizing in protein structure based on the resultant amino acid substitution. We estimated the significant substitution effect of p.A41P on the energy stabilization of Myf5 protein structure. Then, we demonstrated that the mutation in Yorkshire population significantly affected muscle fiber type I composition (p

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