Effects of Defaunation on Fermentation Characteristics, Degradation

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degradation of ryegrass hay and CH4 (methane) production by rumen microbes ... alteration of microbial communities and further medicate the fermentation ... volume. The rumen fluid was strained through 12 layers of cheesecloth to remove the feed particles. ... incubation time was calculated from the remained portion.
Journal of the Korean Society of Grassland and Forage Science 34(3) : 193~201, 2014 http://dx.doi.org/10.5333/KGFS.2014.34.3.193

ISSN 2287-5824 (Print) ISSN 2287-5832 (Online)

Effects of Defaunation on Fermentation Characteristics, Degradation of Ryegrass Hay and Methane Production by Rumen Microbes In Vitro When Incubated with Plant Oils 1

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Wei-Ze Qin , Cheng-Yun Li , Seong-Ho Choi3, Shinekhuu Jugder4, Hyun-Ju, Kim3, Sang-Suk Lee5 and Man-Kang Song3* 1

Institute of Animal Science, Yanbian academy of agricultural sciences, Longjing, Jilin, China, 2

Department of Animal Science, Yanbian University, Jilin, China,

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Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, 361-763 Korea,

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The School of Biological Resource and Management, Mongolian State University of Agriculture, Ulaanbaatar, Mongolia, 5

Department of Animal Science, Suncheon National University, Suncheon, Cheonnam, Korea

ABSTRACT This study was conducted to examine the effects of defaunation (removal of live protozoa) on fermentation characteristics, degradation of ryegrass hay and CH4 (methane) production by rumen microbes when incubated with plant oils (SO, sunflower oil and LO, linseed oil) in vitro. Sodium lauryl sulfate (0.000375 g/ml) as a defaunation reagent was added into the culture solution and incubated anaerobically up to 24 h at 39℃. pH from defaunation was increased for all treatments from 6 h incubation times (p