Effects of resistive exercise and stretching on the

ORIGINAL RESEARCH

DOI: 10.1590/1809-2950/14720923012016

74

Effects of resistive exercise and stretching on the soleus muscle of ovariectomized rats Efeitos do exercício resistido e alongamento no músculo sóleo de ratas ovariectomizadas

Efectos del ejercicio resistido y estiramiento en el músculo sóleo de ratas ovariectomizadas Julye Leiko Ywazaki1, Ana Paula Cunha Loureiro2, Talita Gianello Gnoato Zotz3, Luana Ribeiro Nascimento4, Douglas Vizzu Nobre4, Anna Raquel Silveira Gomes5

ABSTRACT | This study analyzed the effects of resistive

increased the muscle length and the serial sarcomere

exercise and/or stretching in the muscle morphology

number. OH presented the smallest MFCSA compared

of ovariectomized rats. Seventy-five female Wistar

to

rats (218±22g) were divided into 8 groups: Control

sarcopenia, but the exercise’s protocols were enough to

(CONTROL, n=5); Ovariectomy/Hysterectomy (OH, n=10):

prevent the loss of MFCSA. The increase in body weight

ovariectomized/hysterectomized and then waited 8 weeks

associated to resistive training and stretching enhanced

for the onset of osteopenia. Stretching (STRET, n=10):

sarcomerogenesis of ovariectomized rats.

manual stretching of the soleus muscle (10 repetitions

Keywords | Muscle Stretching Exercises; Musculoskeletal

of 60s), twice weekly; Ovariectomy/Hysterectomy and

System; Sarcopenia; Rats.

all

groups.

Ovariectomy/hysterectomy

induced

Stretching (OH+STRET, n=10); Progressive Loading (PL, n=10): 4 sets of 10 repetitions, initial load 50% of body

RESUMO | O estudo analisou os efeitos do exercício

weight, increasing weekly until 70%, three times a week.

resistido e/ou alongamento na morfologia muscular

Progressive Loading and Stretching (PL+STRET, n=10);

de ratas ovariectomizadas. Setenta e cinco ratas Wistar

Ovariectomy/Hysterectomy and Progressive loading

(218±22g) foram divididas em 8 grupos: Controle

exercise (OH+PL, n=10); Ovariectomy/Hysterectomy,

(CONTROLE, n=5); Ovariectomia/histerectomia (OH,

Progressive Loading and Stretching (OH+PL+STRET, n=10).

n=10): foram submetidas à ovariectomia/histerectomia e

Stretching and/or progressive loading were performed for

aguardaram oito semanas para instalação de osteopenia;

6 weeks. After 14 weeks, the female rats were euthanized

Alongamento (ALONG, n=10): alongamento manual do

to remove the soleus muscle. The following variables

músculo sóleo (10 repetições de 60s), duas vezes por

were assessed: body weight; muscle weight and length;

semana; Ovariectomia/histerectomia e Alongamento

number and length of sarcomeres and the muscle fiber

(OH+ALONG, n=10); Exercício Resistido (ER, n=10): 4 séries

cross-sectional area (MFCSA). Comparisons between the

de 10 repetições, carga inicial de 50% do peso corporal,

groups were performed with ANOVA one-way post hoc

aumentando semanalmente até 70%, três vezes por

Tukey or Kruskall-Wallis (p