Electrochemical Homogeneous Enzyme ... - Clinical Chemistry

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Jul 15, 1991 - Robertson FW, Cumming AM. Effects of apoprotein B poly- morphism on serum ... J.Pesce, H. Brian HaIsall,”3 and William. R. Heineman”3.
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CUN. CHEM.39/7, 1432-1434 (1993)

Electrochemical Homogeneous Enzyme Immunoassay of Theophylline in Hemolyzed, Icteric, and Lipemic Samples Haiou

Yao,’

Sarah

We demonstrate

H. Jenkins,2

Amadeo

J.Pesce,

H. Brian

here an electrochemical homogeneous

enzyme immunoassay for theophylline, which can be performed in hemolyzed, lipemic, and ictenc samples. The assay used an unmodified Syva EMIT” theophylline kit.

One of the enzymatic reaction products, NADH, reacted with 2,6-dichloroindophenol (DCIP) to reduce DCIP to DCIPH2, which was detected electrochemically with flowinjection analysis. The inter- and intraassay coefficients of variation of this manual technique were