Randles-Sevcik plot of (A) HQ and (B) CC, for Pd/ImS3-14/(FeIIICuII)/GCE with a scan rate in range of 25–200 mV s-1. (A). (B). 0.10. 0.15. 0.20. 0.25. 0.30. 0.35.
Electronic Supplementary Material (ESI) for Analyst This journal is © The Royal Society of Chemistry 2012
Electronic Supplementary Information
Bio-inspired sensor based on surfactant film and Pd nanoparticles
Eduardo Zappa, Franciane D. Souzab, Bruno S. Souzab, Faruk Nomeb, Ademir Nevesc, Iolanda C. Vieira a,*
a
Laboratory of Biosensors, Department of Chemistry, Federal University of Santa Catarina, 88040-
900, Florianópolis, SC, Brazil. b
Catalysis and Interfacial Phenomena Laboratory, Department of Chemistry, Federal University of
Santa Catarina, 88040-900, Florianópolis, SC, Brazil. c
Laboratory of Bioinorganic and Crystallography, Department of Chemistry, Federal University of
Santa Catarina, 88040-900, Florianópolis, SC, Brazil.
1
Electronic Supplementary Material (ESI) for Analyst This journal is © The Royal Society of Chemistry 2012
(A)
(B)
Fig. S1. SEM micrograph of (A) bare GCE (B) and GCE coated with Pd/ImS3-14 observed at 10 kV with magnification of 10K.
2
Electronic Supplementary Material (ESI) for Analyst This journal is © The Royal Society of Chemistry 2012
12.0
8.0 6.0
(A)
9.0 6.0
4.0 2.0
-6
ip x 10 (A)
-6
ip x 10 (A)
(B)
0.0 -2.0
3.0 0.0 -3.0 -6.0
-4.0
-9.0 -6.0
-12.0 0.10
0.15
0.20
0.25 1/2
v
0.30 1/2
(V
0.35 -1/2
s )
0.40
0.45
0.50
0.15
0.20
0.25
v
0.30 1/2
0.35
0.40
0.45
0.50
-1 1/2
(V s )
Fig. S2. Randles-Sevcik plot of (A) HQ and (B) CC, for Pd/ImS3-14/(FeIIICuII)/GCE with a scan rate in range of 25–200 mV s-1.
3
Electronic Supplementary Material (ESI) for Analyst This journal is © The Royal Society of Chemistry 2012
140
0.025
(A)
(B)
120 0.020 -1
1 / i (A )
-i (A)
100
80
60
0.015
0.010
40 0.005
0
1
2
3
4
5
-3
[Hydroquinone] x10 mol L
0
6
500
-1
1000
1500
2000 -1
1 / [Hydroquinone] (L mol ) 0.12
50
(C)
(D)
0.10 40
-1
1 / i (A )
- i (A)
0.08 30
20
0.06
0.04
0.02 10
0.00 0
1
2
3
4 -4
[Catechol] x10 mol L
5
6
0
-1
5000
10000
15000
20000
-1
1 / [Catechol] (L mol )
Fig. S3. Kinetic activity of complex as catecholoxidase and Lineweaver-Burk plot using solutions of HQ (A and B) and CC (C and D) in phosphate buffer (0.1 mol L-1, pH 7.0).
4
Electronic Supplementary Material (ESI) for Analyst This journal is © The Royal Society of Chemistry 2012
-1
(A)
a
0 1
b
3
6 5
4
4
5 6
k
-i (A)
- i (A)
2
3 2 1
7
0
8
0
2
4
6
8
10
12
14
-6
16
18
20
-1
[Hydroquinone] x10 (mol L )
-0.6
-0.4
-0.2
0.0
0.2
0.4
0.6
0.8
1.0
E (V) vs. Ag/AgCl -1
a
(B) 0
b 1 6
5
3
4
- i (A)
- i (A)
2
4
3
2
1
5
0
k
6 -0.6
0
2
4
6
8
10
12 -6
14
[Catechol] x10 mol L
-0.4
-0.2
0.0
0.2
0.4
0.6
0.8
16
18
20
-1
1.0
1.2
E (V) vs. Ag/AgCl Fig. S4. Square-wave voltammograms obtained using the Pd/ImS3-14/(FeIIICuII)/GCE sensor in (a) phosphate buffer solution only (0.1 mol L-1, pH 7.0) and in solutions containing diphenol in the range of 4.99x10-7 to 1.85x10-5 mol L-1 (curves b-k). The insert shows the calibration curve. (A) HQ; (B) CC.
5
Electronic Supplementary Material (ESI) for Analyst This journal is © The Royal Society of Chemistry 2012
Table S1. CC an HQ recovery in cigarette filter extracts using the proposed sensor
CC (x10-6 mol L-1) Sample
A
B
C
a b
HQ (x10-6 mol L-1)
Added
Founda
Recovery (%)b
Added
Founda
Recovery (%)b
1.87
1.78±0.17
98.20±3.74
1.87
1.72±0.10
91.67±5.40
3.70
3.92±0.13
105.90±3.53
3.70
4.09±0.15
99.42±4.09
5.50
5.41±0.03
98.40±0.51
5.50
5.80±0.04
105.55±3.17
1.87
1.97±0.04
105.30±1.91
1.87
1.79±0.04
95.70±2.02
3.70
3.88±0.12
104.80±3.34
3.70
3.60±0.05
97.30±1.29
5.50
5.48±0.05
99.60±0.84
5.50
5.49±0.05
99.80±0.96
1.87
1.91±0.01
102.10±1.91
1.87
1.93±0.07
103.20±3.50
3.70
3.83±0.06
103.50±3.83
3.70
3.73±0.21
100.80±0.96
5.50
5.31±0.11
96.60±5.31
5.50
5.56±0.11
101.10±2.08
Mean ± standard deviation; n=3. Recovery = (mean found value / added value) x 100%.
6
