Abstracts of The 17th Royan International Congress on Reproductive Biomedicine
'HSDUWPHQW RI +HPDWRORJ\ )DFXOW\ RI 0HGLFDO 6FLHQFH7DUbiat Modares University,Tehran,Iran
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[email protected] Background: Despite widespread application of ART, current rate of pregnancy remains unsatisfactory. Stressful condition of in vitro embryo culture could not completely mimic the in vivo situation that can be itself a probable reason for these undesirable results. Although, a growing body of literature suggests that a short-term exposure of oocytes to a stressor such as hydrostatic pressure or osmotic stress might induce stress tolerance in embryos and improves embryo development. So, in this study we aimed to investigate effect of shortterm exposure of cumulus–oocyte complexes (COCs) to hydrogen peroxide (H2O2) during in vitro maturation on subsequent embryo development. 0DWHULDODQG0HWKRGV Aspirated COCs were matured for 24 h, at the last hour of maturation; oocytes were subjected to different concentrations 10 (10-H2O2), 100 (100-H2O2) and 1000 (1000-H2O2) μM of H2O2. Two cohorts of oocytes were also assigned as control groups; a group without H2O2 (Control) and the second one without H2O2 and sodium pyrovate (Negative control). After maturation, oocytes from each group were evaluated for nuclear maturation. A group of oocytes were subjected to parthenogenetic activation followed by in vitro embryo culture for assessing their developmental parameters. 5HVXOWV Short-term exposure to H2O2 had no effect (P>0.05) on nuclear maturation (65% (control), 64% (Negative control), 68% (10-H2O2), 64% (100-H2O2), and 69% (1000-H2O2). Although, the blastocyst formation rate increased (P
