Chronic exposure, covering 30 generations, led to (i) decreased cell numbers of .... propranolol b a. A A A A. A A A A. B B B B. B B B B. a a a a. a a a a.
Environmental concentrations of pharmaceuticals directly affect phytoplankton and effects propagate through trophic interactions Malgorzata Grzesiuk, Elly Spijkerman, Sabrina C. Lachmann and Alexander Wacker We investigated the effects of chronic exposure of pharmaceuticals (fluoxetine, ibuprofen, and propranolol) on Acutodesmus obliquus and Nannochloropsis limnetica. Moreover, potential modulation of the effects by additional inorganic phosphorus (Pi) limitation, and a potential propagation of the pharmaceuticals’ effect across a trophic interaction was examined. Chronic exposure, covering 30 generations, led to (i) decreased cell numbers of A. obliquus in the presence of fluoxetine (under Pi-replete conditions) (ii) increased carotenoid to chlorophyll ratios in N. limnetica (under Pi-limited conditions), and (iii) increased photosynthetic yields in A. obliquus (in both Pi-conditions). In addition, ibuprofen affected both algae and their consumer: feeding ibuprofen-contaminated algae to Pi-stressed D. magna improved their survival. Here we present tables and additional figures showing results obtained on algae.
Suppl. Table 1 Results of Analysis of Variance (ANOVA) for parameters of A. obliquus and N. limnetica cultured under different Pi-conditions exposed to fluoxetine, ibuprofen and propranolol, using pharmaceuticals and Pi-conditions as factors. Values in bold indicate statistical significance. Variable Factor Cell number Pi-limitation Pharmaceutical Pi x Pharm Cellular chlorophyll a content Pi-limitation Pharmaceutical Pi x Pharm Carotenoid : chlorophyll ratio Pi-limitation Pharmaceutical Pi x Pharm Maximum quantum yield Pi-limitation Pharmaceutical Pi x Pharm F’0 Pi-limitation Pharmaceutical Pi x Pharm F’m Pi-limitation Pharmaceutical Pi x Pharm C:N ratio Pi-limitation Pharmaceutical Pi x Pharm C:P ratio Pi-limitation Pharmaceutical Pi x Pharm Alpha Pi-limitation Pharmaceutical Pi x Pharm
