Essential Cell Biology

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Chapter 7. From DNA to Protein: How Cells. Read the Genome. Essential ... Compe on is a worldwide Synthe c Biology compe on aimed at ... Page 12 ...
Essential Cell Biology



Third Edition

Chapter 7 From DNA to Protein: How Cells Read the Genome

Copyright © Garland Science 2010

The  Interna*onal  Gene*cally  Engineered  Machine  (iGEM)   Compe**on  is  a  worldwide  Synthe*c  Biology  compe**on   aimed  at  undergraduate  university  students.  

130 Teams

In 2010!

(but no UCSB )

Summary of Achievements

Characterization of new  mammalian activators & repressors

Complex mammalian  logic circuit

Computational design of multicellular behavior

Adhesion of mammalian cells

Engineering of Notch-Delta communication system

Figure 7-1 Essential Cell Biology (© Garland Science 2010)

NOTE: [mRNA] weakly correlates

with [protein]

Figure 7-2 Essential Cell Biology (© Garland Science 2010)

DNA  Microarrays  Can  be  Used  to  Measure  mRNA  species  Abundance  

There are two primary differences between DNA and RNA

Figure 7-3 Essential Cell Biology (© Garland Science 2010)

Figure 7-5 Essential Cell Biology (© Garland Science 2010)

Table 7-4 Essential Cell Biology (© Garland Science 2010)

Figure 7-7 Essential Cell Biology (© Garland Science 2010)

Efficient transcription enables many transcripts to be made from one gene simultaneously

Figure 7-8 Essential Cell Biology (© Garland Science 2010)

Table 7-1 Essential Cell Biology (© Garland Science 2010)

In bacteria sigma factors direct RNApol to promoters

Figure 7-9 Essential Cell Biology (© Garland Science 2010)

The sequence features of promoters are conserved

Figure 7-10 Essential Cell Biology (© Garland Science 2010)

Consensus -10 and -35 sequences

Figure 7-11 Essential Cell Biology (© Garland Science 2010)

Table 7-2 Essential Cell Biology (© Garland Science 2010)

Figure 7-12 Essential Cell Biology (© Garland Science 2010)

Figure 7-13 Essential Cell Biology (© Garland Science 2010)

Figure 7-14 Essential Cell Biology (© Garland Science 2010)

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Figure 7- 23a Essential Cell Biology (© Garland Science 2010)

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Figure 7-29 (part 1 of 2) Essential Cell Biology (© Garland Science 2010)

Figure 7-29 (part 2 of 2) Essential Cell Biology (© Garland Science 2010)

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Figure 7-33 (part 2 of 5) Essential Cell Biology (© Garland Science 2010)

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Figure 7-35 (part 1 of 5) Essential Cell Biology (© Garland Science 2010)

Figure 7-35 (part 2 of 5) Essential Cell Biology (© Garland Science 2010)

Figure 7-35 (part 3 of 5) Essential Cell Biology (© Garland Science 2010)

Figure 7-35 (part 4 of 5) Essential Cell Biology (© Garland Science 2010)

Figure 7-35 (part 5 of 5) Essential Cell Biology (© Garland Science 2010)

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Figure 7-37 (part 1 of 3) Essential Cell Biology (© Garland Science 2010)

Figure 7-37 (part 2 of 3) Essential Cell Biology (© Garland Science 2010)

Figure 7-37 (part 3 of 3) Essential Cell Biology (© Garland Science 2010)

Figure 7-38 Essential Cell Biology (© Garland Science 2010)

Ribosomes are recruited to the Kozak Sequence

Table 7-3 Essential Cell Biology (© Garland Science 2010)

Figure 7-40 Essential Cell Biology (© Garland Science 2010)