Essential Cell Biology

Essential Cell Biology



Third Edition

Chapter 6 DNA Replication, Repair, and Recombination

Copyright © Garland Science 2010

Figure 6-2 Essential Cell Biology (© Garland Science 2010)

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Incorrectly inserted nucleotides are removed by DNA Pol

5’-> 3’ editing activity

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With proofreading, error rate is 1 error in 107 bases

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The replacement of RNA in DNA requires three enzymes

Nuclease,

DNA polymerase

DNA ligase

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Single base-pair changes in DNA give rise to many diseases

Also……   Hun$ngton’s  Disease   Hemacrhomatosis   Cys$c  fibrosis   Muscular  Dystrophy   Haemophilia  A  &  B   Achonroplasia   And  many  others!  

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~1013 - 1014 cells in human body

Accumula+on  of  muta+ons  increases  cancer  risk  

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The efficient synthesis of DNA has enabled synthesis of microbial genomes

See interview on “60 Minutes”

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Wear  a  hat  with  a  wide  brim  

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Deaminda+on  of  Cytosine  Yields  Uracil  and  a    permanent  G-­‐>A  muta+on  

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Depurina+on  results  in  base-­‐pair  dele+on  

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