These are not the final page numbers. European Journal of. Immunology. Supporting Information for. DOI 10.1002/eji.201747127. Alaitz Aranburu, Nina H¨o¨ok, ...
These are not the final page numbers
European Journal of Immunology Supporting Information for DOI 10.1002/eji.201747127 Alaitz Aranburu, Nina Ho¨ ok, ¨ Natalija Gerasimcik, Bjorn Corleis, Weicheng Ren, Alessandro Camponeschi, Hans Carlsten, Ola Grimsholm and Inga-Lill Martensson ˚ Age-associated B cells expanded in autoimmune mice are memory cells sharing H-CDR3-selected repertoires
C 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
www.eji-journal.eu
1
1
A
B Before sort
Enrichment of CD21-/low (GC B cells and ABCs) CD19+CD93- B cells
CD19+CD93- B cells
19
CD21
-/low
B cells
ABC
GC
74
13
BSC
CD21
CD21
93
CD95
CD23
CD23
C ABC
GC
100 CD21
CD21-/low
BSC
CD23 97 CD95
0
ABC (CD95low)
GC B cells hi (CD95 )
N.A.
Supplemental Figure 1. Sorting strategy of ABC (CD95low) and GC B cells (CD95high) within CD21-/low B cells. (A) Flow cytometry plot shows CD21-/low B cells before sort. (B) Gating strategy for the enrichment of CD21-/low B cells, including GC B cells and ABCs. (C) Sorting and purity for CD21-/low B cells, ABCs and GC B cells. n.a., not analyzed. Spleens pooled from 5 SLC-/- mice.
1
1
Supplemental Table 1. Antibodies for the flow cytometry used in this study. Antibody CD11c-PeCy7 CD11c-PerCP CD16/32 CD19-BV450 CD19-BV510 CD21-APC-Cy7 CD21-BV421 CD21-FITC CD23-BV510 CD23-PE CD23-PerCP-Cy5.5 CD38-PE CD43-APC CD73-PeCy7 CD80-biotin CD86-biotin CD93-APC CD95-BB515 CD95-biotin CD138-PE CD184(CXCR4)-biotin CD273(PDL2)-BV421 GL7-AF647 GL7-eFl450 GL7-FITC IgM-AF488 MHC class II-biotin Streptavidin-PE Streptavidin-PerCP
