Evolution of the shut-off steps of vertebrate

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L L LLL LLL LLL LLL LLL LLL LLL LLL LLL LLLL ...... NDLRRES- ILE- PPP ...... U U U U |. IT AL LLLL LLL LLL LLL LLL LLL LLL LLL LLL LLL. U U |. Σ Ω Ω rh r5 r6 r5 r6 b tb 1 1 1 1 Մ) ...... A A A A UM L H L L & G A D A A A A K Z Z Z Z A A A A A.
1 Supplementary Tables for

Evolution of the shut-off steps of vertebrate phototransduction Trevor D. Lamb1, Hardip R. Patel2, Aaron Chuah3, and David M. Hunt4,5

1

Eccles Institute of Neuroscience, 2National Centre for Indigenous Genomics, and 3Genome Discovery Unit, John Curtin School of Medical Research, The Australian National University, ACT 2600, Australia, and 4The Lions Eye Institute and 5School of Biological Sciences, The University of Western Australia, WA 6009, Australia.

Contents Page Supplementary Table S1. Summary of transcripts examined.

2

Supplementary Table S2. Multiple sequence alignment for GRKs.

5

Supplementary Table S3. Multiple sequence alignment for arrestins.

11

Supplementary Table S4. Multiple sequence alignment for RGS9/11s.

15

Supplementary Table S5. Multiple sequence alignment for Gβ5s.

21

Supplementary Table S6. Multiple sequence alignment for R9APs.

23

2 Supplementary Table S1. Summary of transcripts examined #

Comp.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

GRK1X GRK1X GRK1A GRK1A GRK1A GRK1A GRK1A GRK1A GRK1A GRK1B GRK1B GRK1B GRK1B GRK1B GRK7 GRK7 GRK7 GRK7 GRK7 GRK7 GRK7 GRK7-1 GRK7-1 GRK7-2 GRK7-2

26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45

Arr-S1 Arr-S1 Arr-S1 Arr-S1 Arr-S1 Arr-S2 Arr-S2 Arr-S2 Arr-S2 Arr-S2 Arr-S Arr-S Arr-VY Arr-VY Arr-VX Arr-VX Arr-C Arr-C Arr-C Arr-C

Label on Figures

GRK1X G.australis 57773-1-1 GRK1X M.mordax 42166-5-1 GRK1A Western ray 41513-9-2 [ GRK1A Eastern ray 24652-2-1 ] GRK1A Bluespot ray 29212-2-1 GRK1A Bamboo shark 8046-1-1 GRK1A Reef shark 53758-1-1 GRK1A Florida gar 24879-1-1 GRK1A Bowfin 29529-1-1 GRK1B Western ray 34447-1-1 [ GRK1B-Partial Eastern ray 22403-1-1 ] GRK1B Bluespot ray 4032-1-1 GRK1B Bamboo shark 26651-1-1 GRK1B Bowfin 26676-4-1 GRK7 Western ray 39734-1-1 [ GRK7 Eastern ray 24666-1-1 ] GRK7 Bluespot ray 37188-7-2 GRK7 Bamboo shark 35291-1-1 GRK7 Reef shark 15561-1-1 GRK7 Florida gar 30401-2-1 GRK7 Bowfin 27257-5-2 GRK7-1 G.australis 58623-2-1w1-2 GRK7-1 M.mordax 40500-6-1 GRK7-2 E.cirrhatus 127887-1-1 GRK7-2-Non-contig G.australis 26295-2-1w27699-1-1 Arr-S1 Western ray 26221-2-1 [ Arr-S1 Eastern ray 41685-1-1 ] Arr-S1 Bluespot ray 30422-1-1 Arr-S1 Bamboo shark 45077-7-1 Arr-S1 Reef shark 55938-25-1 Arr-S2 Western ray 22644-1-1 [ Arr-S2 Eastern ray 20756-1-1 ] Arr-S2 Bluespot ray 31414-1-1 Arr-S2 Bamboo shark 28962-1-1 Arr-S2 Reef shark 55253-10-3 Arr-S Florida gar 22184-1-1 Arr-S Bowfin 24232-1-2 Arr-VY E.cirrhatus 129805-1-1 Arr-VY G.australis 54008-1-2 Arr-VX G.australis 59149-4-1 Arr-VX M.mordax 42250-9-1 Arr-C Western ray 37242-1-1 [ Arr-C Eastern ray 20877-1-1 ] Arr-C Bluespot ray 36257-4-1 Arr-C Bamboo shark 45371-8-2

Accession number

aa's

MG063622 MG063623 MG063624 MG063625 MG063626 MG063627 MG063628 MG063629 MG063630 MG063631 MG063632 MG063633 MG063634 MG063635 MG063636 MG063637 MG063638 MG063639 MG063640 MG063641 MG063642 MG063643 MG063644 MG063645 MG063646

571 569 564 564 564 565 565 563 563 558 500 558 559 561 554 554 554 552 555 550 551 556 553 549 486

MG063647 MG063648 MG063649 MG063650 MG063651 MG063652 MG063653 MG063654 MG063655 MG063656 MG063657 MG063658 MG063659 MG063660 MG063661 MG063662 MG063663 MG063664 MG063665 MG063666

390 390 390 384 383 427 427 420 422 422 391 392 385 405 392 391 394 394 394 394

Join

J

M

*

RPKM

RPKM -CDS

M M M M M M M M M M M M M M M M M M M M M M M M

* * * * * * * * * *

18.1 37.1 104.7 13.8 191.0 270.5 354.5 248.2 264.1 8.0 4.4 37.3 2.9 21.1 160.6 184.0 62.6 339.8 384.3 27.2 73.6 8.8 25.6 40.7 6.4

13.2 32.5 107.5 13.3 171.8 315.7 449.6 206.8 322.3 8.7 4.7 41.0 2.8 21.8 182.3 58.1 75.3 466.6 437.0 27.2 111.4 18.4 27.7 55.0 6.4

M M M M M M M M M M M M M M M M M M M M

* * * * * * * * * * * * * * * * * * * *

1189.5 2745.4 3503.3 2115.6 2475.3 384.5 125.2 746.8 931.8 228.3 1390.9 1527.0 431.6 976.0 988.0 921.9 145.9 319.1 198.7 79.7

2436.4 687.7 4155.8 2268.8 4997.8 461.4 175.0 852.2 1104.3 232.1 1199.4 2067.2 626.4 1170.1 1243.1 1039.0 268.3 50.9 205.3 87.5

* * * * * * * * * * * * *

NJ

3 46 47 48

Arr-C Arr-C Arr-BX

49 50

Arr-BX Arr-BY

51 52 53 54 55 56 57 58

Arr-BY Arr-B1 Arr-B1 RGS9 RGS9 RGS9 RGS9 RGS9

59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93

RGS9 RGS9 RGS9 RGS9 RGS9 RGS11 RGS11 RGS11 RGS11 RGS11 RGS11 RGS11 RGS11 RGS11 RGS11 Gb5L Gb5L Gb5L Gb5L Gb5L Gb5L Gb5L Gb5L Gb5L Gb5L R9AP R9AP R9AP R9AP R9AP R9AP R9AP R9AP R9AP R9AP

Arr-C Florida gar 15939-1-1 Arr-C Bowfin 14016-1-1 Arr-BX-Partial G.australis 54326-4-1w1-1w2-3 Arr-BX-Partial M.mordax 41599-4-1 Arr-BY-Non-contig G.australis 41931-1-1w37543-2-1w1-1w53942-1-1 Arr-BY M.mordax 41599-5-1 Arr-B1 Florida gar 28267-2-1w1-11 Arr-B1 Bowfin 26818-1-6 RGS9 E.cirrhatus 137654-1-3 RGS9 G.australis 56905-1-2 RGS9 M.mordax 34174-1-2 RGS9 Western ray 41046-8-8 [ RGS9-Non-contig Eastern ray 1867-2-1w1-1 ] RGS9 Bluespot ray 37076-2-1 RGS9 Bamboo shark 45486-8-1 RGS9 Reef shark 51239-1-1 RGS9 Florida gar 21856-1-2 RGS9 Bowfin 26690-1-1 RGS11 E.cirrhatus 136920-1-2 RGS11 G.australis 47497-1-2 RGS11 M.mordax 39562-1-2 RGS11 Western ray 41178-3-3 [ RGS11-Partial Eastern ray 22212-1-3 ] RGS11 Bluespot ray 32308-1-2 RGS11 Bamboo shark 41815-1-3 RGS11 Reef shark 50711-1-1 RGS11 Florida gar 29521-1-2 RGS11 Bowfin 26311-1-2w1-5 Gb5L E.cirrhatus 130391-1-2 Gb5L G.australis 57438-2-2 Gb5L M.mordax 41947-2-2 Gb5L Western ray 37263-2-2 [ Gb5L Eastern ray 13249-1-1 ] Gb5L Bluespot ray 28709-1-1 Gb5L Bamboo shark 37645-2-1 Gb5L Reef shark 40872-1-1 Gb5L Florida gar 16504-1-1 Gb5L Bowfin 22627-1-1 R9AP E.cirrhatus 138946-5-1 R9AP G.australis 56404-2-1 R9AP M.mordax 41679-5-1 R9AP Western ray 30726-1-1 [ R9AP Eastern ray 17388-1-1 ] R9AP Bluespot ray 25945-2-2 R9AP Bamboo shark 37642-1-1 R9AP Reef shark 40575-1-1 R9AP Florida gar 28835-2-1 R9AP Bowfin 1668-1-1

MG063667 MG063668 MG063669

388 386 332

M M J

MG063670 MG063671

243 354

NJ

MG063672 MG063673 MG063674 MG063675 MG063676 MG063677 MG063678 MG063679

423 418 418 498 478 478 490 478

MG063680 MG063681 MG063682 MG063683 MG063684 MG063685 MG063686 MG063687 MG063688 MG063689 MG063690 MG063691 MG063692 MG063693 MG063694 MG063695 MG063696 MG063697 MG063698 MG063699 MG063700 MG063701 MG063702 MG063703 MG063704 MG063705 MG063706 MG063707 MG063708 MG063709 MG063710 MG063711 MG063712 MG063713 MG063714

490 490 491 489 487 433 419 413 465 391 467 465 461 471 469 395 395 395 395 395 395 395 395 395 395 246 238 243 244 244 238 239 234 238 238

J

NJ

* * *

1139.6 2604.9 18.3

1172.2 3140.9 19.4

M

* *

46.7 5.5

49.8 6.1

M M M M M M M M

* * * * * * * *

24.7 11.8 19.5 128.9 50.8 8.8 23.1 3.2

26.4 7.2 12.9 156.7 56.7 10.0 25.4 3.3

M M M M M M M M M

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *

83.9 51.1 70.6 112.7 74.5 5.6 13.0 2.8 26.4 15.9 32.7 10.3 9.3 7.6 18.9 72.0 37.7 30.5 127.0 141.0 186.6 90.0 221.0 102.4 41.0 7.7 7.9 16.0 19.6 25.9 41.4 8.7 4.8 27.3 36.0

96.6 56.1 81.9 85.9 60.7 6.1 15.7 6.2 28.3 16.3 29.2 10.2 10.9 5.3 31.0 94.2 55.9 33.2 137.6 17.3 198.6 95.6 252.4 66.4 77.3 4.7 9.8 12.7 30.0 21.9 51.9 9.7 5.5 52.3 63.4

M M

J

M M M M M M M M M M M M M M M M M M M M M M

4 94 95 96 97 98 99

R9AP-B R9AP-B R9AP-B R9AP-B R9AP-B R9AP-B

R9AP-B Western ray 39739-1-1 [ R9AP-B Eastern ray 23740-1-1 ] R9AP-B Bluespot ray 37118-18-1 R9AP-B Bamboo shark 45543-9-1 R9AP-B Reef shark 612-1-1 R9AP-B Florida gar 25574-1-1

MG063715 MG063716 MG063717 MG063718 MG063719 MG063720

254 254 254 255 254 254

M M M M M M

* * * * * *

21.0 31.2 41.7 59.7 140.0 44.2

46.2 4.0 58.7 112.8 213.4 41.7

Notes, and explanation of columns Column 3, ‘Label on Figures’: Sequences are indicated ‘-Partial’ if they included fewer than 95% of the expected residues. Entries in square brackets do not appear in phylogenies because the protein sequence was identical to another sequence included in the tree; for example, all entries for Eastern ray were omitted in favour of the identical protein sequences for Western ray. Column 6, ‘Join’: An entry ‘J’ indicates that the sequence was manually joined from more than one contig. These joined sequences overlapped, except where indicated ‘NJ’ for a noncontiguous join. Column 7: An entry ‘M’ indicates that the sequence started with a Met. Column 8. An entry ‘*’ indicates that sequence ended with a stop codon. Column 9: RPKM = Reads per kilobase per million mapped reads. Column 10: RPKM-CDS = Same, but calculated across only the coding region of the transcript.

GRK7_Human_NP-631948 GRK7_Cattle_NP-776757 GRK7_Opossum_XP-001364799 GRK7_Tasmanian_devil_XP-003766156 GRK7_Chicken_XP-426681 GRK7_Pigeon_XP-005505950 GRK7_Ground-tit_XP-005527115 GRK7_Anole_XP-003218299 GRK7_Gecko_XP-015268958 GRK7_Xenopus_NP-001120573 GRK7_Nanorana_XP-018415869 GRK7_Florida_gar_30401-2-1 GRK7_Bowfin_27257-5-2 GRK7a_Zebrafish_NP-001027011 GRK7a_Medaka_NP-001098131 GRK7b_Zebrafish_NP-001028262 GRK7b_Medaka_XP-004075863 GRK7_Reef_shark_15561-1-1 GRK7_Bamboo_shark_35291-1-1 GRK7_Bluespot_ray_37188-7-2 GRK7_Western_ray_39734-1-1 GRK7_Elephant_shark_XP-007891889 GRK7-1_M.mordax_40500-6-1 GRK7-1_G.australis_58623-2-1w1-2 GRK7-1_P.marinus_S4RD08 GRK7-2-Non-contig_G.australis_26295-2-1w27699-1-1 GRK7-2_P.marinus_S4RL90 GRK7-2_L.camtschaticum_JL9580 GRK7-2_E.cirrhatus_127887-1-1 GRK-Trimmed_C.intestinalis_XP-009860997 GRK-Partial_C.savignyi_H2YPC9 GRK1X_M.mordax_42166-5-1 GRK1X_G.australis_57773-1-1 GRK1X-Partial_L.camtschaticum_JL8961 GRK1B_Chicken_NP-990026 GRK1B_Ground-tit_XP-005533433 GRK1B_Golden_eagle_XP-011599401 GRK1B_Turtle_XP-006112219 GRK1B_Anole_XP-003228556 GRK1B_Bearded_dragon_XP-020665279 GRK1B_Spotted_gar_XP-006627541 GRK1B_Bowfin_26676-4-1 GRK1B_Pike_XP-010901292 GRK1B_Asian_bonytongue_XP-018581705 GRK1B_Zebrafish_NP-001017711 GRK1B_Bamboo_shark_26651-1-1 GRK1B_Bluespot_ray_4032-1-1 GRK1B_Western_ray_34447-1-1 GRK1A_Human_NP-002920 GRK1A_Cattle_NP-776598 GRK1A_Opossum_XP-001364656 GRK1A_Tasmanian_devil_XP-003765754 GRK1A_Xenopus_XP-002937018 GRK1A_Nanorana_XP-018428616 GRK1A_Florida_gar_24879-1-1 GRK1A_Bowfin_29529-1-1 GRK1A_Zebrafish_NP-001029353 GRK1A-1_Medaka_NP-001188419 GRK1A-2_Medaka_XP-004081538 GRK1A_Reef_shark_53758-1-1 GRK1A_Bamboo_shark_8046-1-1 GRK1A_Bluespot_ray_29212-2-1 GRK1A_Western_ray_41513-9-2 GRK1A_Elephant_shark_XP-007889799 GRK_C.intestinalis_XP-009859302 GRK_B.floridae_XP-019631688 GRK_B.floridae_XP-002608270 GRK6_Human_NP-002073 GRK5_Human_NP-005299 GRK4_Human_NP-892027

M--------------------VDMGALDNLIANTAYLQARKPSDCD---SKELQRR-RRSLALPGL--QGC-AELRQKL--------------------------------------S---LNFH M--------------------VDMGGLDNLIANTAYLQARKSSDAD---SKELQRR-RRSLMLPGP--QSC-EQLRQAL--------------------------------------A---TDFH M--------------------VDMGGLDNLIANTAYLQARNTGDGD---TKEMQKR-RRSLALPTP--ENC-TELRQVL--------------------------------------P---PHFE M---------------------DLGGLDNLIANTAYLQAR---DGD---TKEMQKR-RRSLALPAR--EHC-AELRQAL--------------------------------------P---THFE M--------------------CDMGGLDNLIANTAYLQARKSGEGD---TKEMQKR-RKSLSLPKI--DQC-GEVRQSI--------------------------------------A---ADYE M--------------------CDMGGLDNLIANTAYLQARKSGEGD---TKEMQKR-RKSLSLPKI--DQC-GEVRQSV--------------------------------------V---ADYD M--------------------CDMGGLDNLIANTAYLQARKSGEGD---TKEMQKR-RKSLSLPKI--EEC-SQHRESI--------------------------------------A---GDYD M--------------------CDMGGLDNLIANTAYLNARKSGDVD---AKELQKR-WRVLALPKI--EEC-AAIKESI--------------------------------------T---MDYN M--------------------CDMGGLDNLIANTAYLQARKSGDAE---NKEIQKR-RKSLALPRI--DEF-TELRNSI--------------------------------------K---VEYE M--------------------CDMGGLDNLIANTAYLQARKNSEGD---AKELQKR-RKSLSLPPP--DVSRKEIKETI--------------------------------------T---LDYQ M--------------------CDMGGLDNLIANTAYLQARKSSEGD---LRELQKR-RKSLSLPPT--DVCRKEIKDTI--------------------------------------P---VDFQ M--------------------CDMGGLDNLIANTAYLQARKSGDGD---TKEMQKR-RRSLSLPKP--EQC-VELRQKT--------------------------------------E---MNFE M--------------------CDMGGLDNLIANTAYLQARKSGDGD---GKEMQKR-RRSLALPKT--DQC-AEIRQNM--------------------------------------D---KNFE M--------------------CDMGGLDNLVANTAYLKAQGG---D---DKEMKKR-RRSLSLPKP--EQC-ASLRTSL--------------------------------------D---KDFE M--------------------CDMGGLDNLVANTAYLKAQGG---D---DKEMKKR-RRSLSLPKP--EQC-VALRESI--------------------------------------E---KDFT MS--------------------DMGGLKNLVANTDYLKAQSL---D---EKEIKKR-RCSLGLPHL--GNC-TDVRASV--------------------------------------S---KGFE MSSSLVICLLPFNMPHDSPPKADPAGLESLVVNTAYLKGQQAG------RNEQRMR-RESLTLPNP--KKS-TAPRAGA--------------------------------------E---KSYE M--------------------CDMGALDNLIANTVYLQARKSVDGD---AKEMRKR-RKSLVLPNH--QQS-ASVRDKI--------------------------------------D---VDFE M--------------------CDMGALDNLIANTVYLQARKSVDGD---FKEMRKR-RQSLALPSH--QQS-ASVKQNI--------------------------------------D---IIYE M--------------------CDMGALDNLIANTIYLQARKSIDGD---IKEMRKR-RQSLFLPSH--AQC-VSVKQSI--------------------------------------D---ITYE M--------------------CDMGALDNLIANTVYLQARKSVDGD---IKEMRKR-RQSLFLPNH--AQC-VSVKQNI--------------------------------------D---VEYE M--------------------CDMGGLDNLIANTAYLQARKSVDGD---IKEMQKR-RRSLSLPSR--DQC-IELRQKI--------------------------------------C---VEYE M--------------------CDMGALDNLIANTAYLEARKSGDGG---SKEAARR-RRSLALPSP--TKC-KSLYKSI--------------------------------------KDSNLEYD M--------------------CDMGGLDNLIANTAYLEARKSGDGG---AKEMARR-RKSLALPPP--AQC-QSLRKSA--------------------------------------DDPSLDYG M--------------------CDMDCLDNLIANTAYLEARKSGDGG---TRD--KA-RRSIVLPTL--TQC-RSLRKTI--------------------------------------PDSSVDYG -----------------------------LIANTAYLEARKTGDGA---GGGGKKR-RCSTALPGPRGARC-AEVRAEV--------------------------------------RGT-ESYA M--------------------CDLGALDNLIANTAYLEARKSGDGA---GGSGKKR-RCSMVLPGPRGAQC-ADMRTKV--------------------------------------AGT-QTYA -----------------------LGALDNLIANTAYLEARKSGDGA---GGSGKKR-RCSMVLPGPRGAQC-VDMRAKV--------------------------------------AGT-QTYA M--------------------CDLGGLDNLIANTAYLQARKSTDAG---VGG-SKR-RRSLALPGP--AKC-MEIRQLL--------------------------------------H---LSFA -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MDIGGLETVVANSAYVAARGDVD---GGSGGRDRKMRARLKLPHV--LQL-EEARARTLALHPEPSNPS---------------------------L---PSFE ---------------------MDIGGLETVVANSAYVAARGDVDGG-GGGGGRDRKMRARLKLPHV--LRL-EEVRARVVQTHPEPAEPA---------------------------L---PSFQ -------------------------------------------------------------------------------------------------------------------------------------------------MDIGGLETVVANSAYVSARGG---G-PGGSARDRKHRARLRLPHI--SQC-EALRARLAGGGLQNGV-AQQDGGPKQDGGPKQDGGPKQDGG-GEEE---ASFR -----------------------IGGLETVVANSAYVSARG----G-PGGGGKDRRSKARLRLPHI--SQC-EALRAQLGGAGAQNG------------GPGAPNGAPPGHTQDGGQD---TSFR ---------------------MDIGGLETVVANSAYVSARG----G-PGASGRDRRQRARLRLPHI--TQC-EALRARLGGGHPPGGTHGAQGGGAQNGGGGGPDGVAAGGGL-AGED---ASFR ---------------------MDIGGLETVVANSAYVSARGSVDAS-AASTLRDKKMRAKLKLPHI--TQC-EHLKDQM--------------------------------------D---LNFQ ---------------------MDIGGLETVVANSAYVSARGD---G-GPSTGRDKKMRAKLKLPHI--TQC-EHLKDTL--------------------------------------D---LDFN ---------------------MDIGGLETVVANSAYVSARGE---G-GPSSGRDKKMRAKLKLPHI--TQC-EHLKDTL--------------------------------------D---LSFN ---------------------MDISGLETVVANSAYVTARGSMDGG-MAATMRDKKMRARLKLPHI--QVC-DHMKATV--------------------------------------D---TGFE ---------------------MDIGGLETVVANSAYVAARGSVDGN-AAATMRDKKMRAKLKLPHI--TVC-EHMKSTV--------------------------------------D---QSFE ---------------------MDMGGLQNIVANSAYVSARGSVDGA-SASAMRDKKFRAKLDLPHI--TKC-MHMKTTV--------------------------------------D---SAFE ---------------------MDIGGLETAVANSAYVSARGSLDGN-AAATMRDKKMRAKLKLPHI--KQC-EHMKTTV--------------------------------------D---SVFD ---------------------MDIGGLETVVANSAYVSARGSIDGA-AAATMRDKKYRSKLNLPHI--RQC-EQMKTIV--------------------------------------D---TSFD ---------------------MDFAGLETVVANSAYLAARGSIDSH-LAFAMQDRRMRARLKLPPV--SQC-QPTEGEM--------------------------------------D---LTFE ---------------------MDFGGLETVVANSAFVAARSSMDAG-SALAVRDKKMRARLRLPPV--SRC-QALRQEL--------------------------------------D---CSFR ---------------------MDFGGLETVVANSAYMAARSSMDSN-SALTMRDKKMRARLKLPPV--AQC-QGLRKEI--------------------------------------D---LTFQ ---------------------MDFGSLETVVANSAFIAARGSFDGS-SSQPSRDKKYLAKLKLPPL--SKC-ESLRDSL--------------------------------------S---LEFE ---------------------MDFGSLETVVANSAFIAARGSFDAS-SGPASRDRKYLARLKLPPL--SKC-EALRESL--------------------------------------D---LGFE ---------------------MDFGSLETVVANSAFIAARGSFDASGSSHLSRDKKYFSKLKLPPL--SKC-ESLRDTL--------------------------------------N---LDFE ---------------------MDFGSLETVVANSAFIAARGSFDASGSSHLARDKKYFSKLKLPPL--SKC-ESLRNTL--------------------------------------N---LDFE ---------------------MDFGALETVVANSAYITARGSFDGGFNPQITRNKKYRARLKLPPL--SEC-EHLKDKL--------------------------------------D---LQFE ---------------------MDFGALETVVANSAYITARGSFDGGFNPQITRNKKFRAKLKLPPL--SDC-EHLKDQL--------------------------------------D---LEFD ---------------------MDIGGLETVVANSAYISARASFDGSSNAAANRDKKYRAKLKLPHI--TQC-EDLKDQL--------------------------------------D---LEFD ---------------------MDIGGLETVVANSAYISARASFDGSSNASATRDKKYRAKLKLPHI--TQC-EDLTEKI--------------------------------------D---LEYD ---------------------MDIGGLTTVVANSAYISARGSFDGTANPASNRDKKYHSKLKLPHI--TVC-EDLRETL--------------------------------------D---LQFR ---------------------MDIGGLTTVVANSAYISARGSFDGTANPAANRDKKFHSRLKLPHI--TVC-EGLRETL--------------------------------------D---LGFQ ---------------------MDIGGLTTVVANSAYINARGSIDG-SNPATARDKKYHSRLKLPHI--TVC-EGLKDTL--------------------------------------D---LAFD ---------------------MDLGNLETVVANSAYISARGSFDGSANLQMTRDKKYRAKLKLPNI--TQS-ETLKDQI--------------------------------------D---VEFE ---------------------MDLGSLQTVVANSAYISARGSFDGSANLQMTRDKKYRAKLKLPHI--TQS-ETLKEKI--------------------------------------D---LAFE ---------------------MDLGSLQTVVANSAYISARVTFDGPSSVQMTRDKKYRAKLKLPHI--TQS-ETLKDQI--------------------------------------D---IEFE ---------------------MDLGSLQTVVANSAYISARGSFDGTANVQMTRDKKYRAKLKLPHI--TQS-ESLKDQI--------------------------------------D---TEFE ---------------------MDIGGLQTVVANSAYISARGSFDGNTNVQMTRDKKFRAKLKLPHI--TQS-ETLKDKI--------------------------------------D---LEFE M--------------------MDME-LENIVANTVYLKAREGGGGK---RKGKSKKWREMMKFPHI--SLT-EDLRRTI--------------------------------------E---PEYT -----------------------ME-LENIVANTVYIKAREGGGGK---RKGKSKKWKLILKFPHI--TEC-EYLRDKI--------------------------------------D---CDYT -----------------------MK-HEVL------------------------------------------------H--------------------------------------D---CDYT -----------------------ME-LENIVANTVLLKAREGGGGN---RKGKSKKWRQMLQFPHI--SQC-EELRLSL--------------------------------------E---RDYH -----------------------ME-LENIVANTVLLKAREGGGGK---RKGKSKKWKEILKFPHI--SQC-EDLRRTI--------------------------------------D---RDYC -----------------------ME-LENIVANSLLLKARQGGYGK---KSGRSKKWKEILTLPPV--SQC-SELRHSI--------------------------------------E---KDYS 1.......10........20........30........40........50........60........70........80........90.......100.......110.......120.....

57 57 57 53 57 57 57 57 57 58 58 57 57 54 54 54 74 57 57 57 57 57 60 60 58 52 61 58 56 68 70 92 79 92 59 56 56 59 59 59 59 59 59 59 59 59 59 60 60 60 60 60 60 60 60 59 60 60 60 60 60 57 54 12 54 54 54

GRK7_Human_NP-631948 GRK7_Cattle_NP-776757 GRK7_Opossum_XP-001364799 GRK7_Tasmanian_devil_XP-003766156 GRK7_Chicken_XP-426681 GRK7_Pigeon_XP-005505950 GRK7_Ground-tit_XP-005527115 GRK7_Anole_XP-003218299 GRK7_Gecko_XP-015268958 GRK7_Xenopus_NP-001120573 GRK7_Nanorana_XP-018415869 GRK7_Florida_gar_30401-2-1 GRK7_Bowfin_27257-5-2 GRK7a_Zebrafish_NP-001027011 GRK7a_Medaka_NP-001098131 GRK7b_Zebrafish_NP-001028262 GRK7b_Medaka_XP-004075863 GRK7_Reef_shark_15561-1-1 GRK7_Bamboo_shark_35291-1-1 GRK7_Bluespot_ray_37188-7-2 GRK7_Western_ray_39734-1-1 GRK7_Elephant_shark_XP-007891889 GRK7-1_M.mordax_40500-6-1 GRK7-1_G.australis_58623-2-1w1-2 GRK7-1_P.marinus_S4RD08 GRK7-2-Non-contig_G.australis_26295-2-1w27699-1-1 GRK7-2_P.marinus_S4RL90 GRK7-2_L.camtschaticum_JL9580 GRK7-2_E.cirrhatus_127887-1-1 GRK-Trimmed_C.intestinalis_XP-009860997 GRK-Partial_C.savignyi_H2YPC9 GRK1X_M.mordax_42166-5-1 GRK1X_G.australis_57773-1-1 GRK1X-Partial_L.camtschaticum_JL8961 GRK1B_Chicken_NP-990026 GRK1B_Ground-tit_XP-005533433 GRK1B_Golden_eagle_XP-011599401 GRK1B_Turtle_XP-006112219 GRK1B_Anole_XP-003228556 GRK1B_Bearded_dragon_XP-020665279 GRK1B_Spotted_gar_XP-006627541 GRK1B_Bowfin_26676-4-1 GRK1B_Pike_XP-010901292 GRK1B_Asian_bonytongue_XP-018581705 GRK1B_Zebrafish_NP-001017711 GRK1B_Bamboo_shark_26651-1-1 GRK1B_Bluespot_ray_4032-1-1 GRK1B_Western_ray_34447-1-1 GRK1A_Human_NP-002920 GRK1A_Cattle_NP-776598 GRK1A_Opossum_XP-001364656 GRK1A_Tasmanian_devil_XP-003765754 GRK1A_Xenopus_XP-002937018 GRK1A_Nanorana_XP-018428616 GRK1A_Florida_gar_24879-1-1 GRK1A_Bowfin_29529-1-1 GRK1A_Zebrafish_NP-001029353 GRK1A-1_Medaka_NP-001188419 GRK1A-2_Medaka_XP-004081538 GRK1A_Reef_shark_53758-1-1 GRK1A_Bamboo_shark_8046-1-1 GRK1A_Bluespot_ray_29212-2-1 GRK1A_Western_ray_41513-9-2 GRK1A_Elephant_shark_XP-007889799 GRK_C.intestinalis_XP-009859302 GRK_B.floridae_XP-019631688 GRK_B.floridae_XP-002608270 GRK6_Human_NP-002073 GRK5_Human_NP-005299 GRK4_Human_NP-892027

SLCEQQPIGRRLFRDFLA-TVPTFRKAATFLEDVQNWELAEEGP---TKDSALQGLVATCA-SAPAPGN--PQPFLSQAVATKCQAA------------TTEEERVAAV-TLAKAEAMAFLQ-EQ SLCEQQPIGRRLFRDFLA-TVPAYQEARGFLEEVQSWELAEEGP---AKGSALQGLVTTCA-AAPVPGR--PHPFFSPALVTKCQAA------------TTDDDRASLV-ELAKAEVMAFLQ-DQ TLCEQQPIGRRLFHDFLA-TVPSYKEAVDFLGEVSTWELAEEDG---TKDSTLKKLVNACA-QASAPTG--QLPFLSPALATRCQVA-------------SEKEHASLV-GLAKAETMAFLQ-NQ SMCEQQPIGRRLFHDFLA-TVPAYKVAVDFLAEVSSWELAEEGS---TKDSTLRGLVQLCA-QAT-PTG--QLPFLSRALATKCQTA-------------SETEHASLV-ELAKAETMTFLQ-DQ SICEQQPIGKRFFRDFLE-TVPENLVARDFLDEVSNWEL-AEDN---IKSSTMENIITNFL-KTGS-KN--YLSFMSSDLVSKCQAA-------------TEKDYENVV-QLARDEAKVFLQ-GK SICEQQPIGKKFFRDFLE-TVPEYLVAKGFLDEVSNWEL-AEDN---AKTSIMENMVTDFL-KTGS-KN--YLAFMSSDLASKCQAA-------------TAKDYENII-QLAKEETKAFLR-DK SICEQQPIGKRFFRDFLE-TVPEYLVARDFLDEVSNWEL-AEDS---LKSSTMENIVTNFL-KEGA-KN--YLSFMSSDLVSKCKAA-------------AAKDYENIM-QLAKEETKVFFK-DK AICEQQPVGKKFFGEFLE-TVPEYLVAKEFMDEVYAWEL-AEDN---VKNSLLEGIINMYL-KNVS-NS--FLKFLSADLSNKCQSV-------------SKDDFEKIV-LSAREETNEYLK-GK HICEKQPIGRNFFKEFVE-SVPEYRLASEFLDEADGWEL-AEDK---IKSSILEGLVNMYL-KNIS-NS--YLKFLSPDLAKKCQAA-------------TKNDFETVM-VLAMEEAKAFLK-EE SICVEQPIGQRLFKDFLA-TVPEYKLAEEFLEEVKDWEL-EEGS---AKEQLMEKLVSRRF-KEPA-EG--SLNFLGKDLSSRIQKG-------------QSKDMPELI-MLAKEAGTAFLM-DA SICVEQPIGRRVFRDFLL-SVPEYLVVQEFLDEVNDWEL-EEGS---AKEQLLQVLMIKRF-KQDS-GE--RLPCFSEDLSKRFQNA-------------QGAEIPPMV-LLAKDEANAYLG-KE NLCEQQPIGKKLFRQFTA-TVPEYQVAIDFLDELNNWDL-AEST---SKDNLRQNITTSFL-KPDS-KN--FIAYLCAELAEKCKNV-------------TEKDFEEVM-EKAKEETRNFLK-GK SLCEQQPIGKKLFRQFAA-TLPVYQVAVDFLDELNNWDL-AESN---SKDNLRQNISTSFL-KSDS-KN--FLSFLSPGLADKCQNV-------------TEKDFEEVT-TKAKEETRSFLK-GK SLCEKQPIGKKLFRQYLSQGGPECTTAAEFLDDLNEWEL-SESA---ARDKARTNIINKFC-KEGS-KS--SLTFLTGDVATKCKAV-------------SDKDFEEVM-GQVKTATKEFLK-GK LLCERQPIGKRLFRDFLA-NTPEFKLAAEFLDELYDWDL-AEGA---AKDKARQNIINKYC-KPDS-KT--FLTFLSGEPAEKCKSV-------------TDATFEEVMKNKVQDGVREFLK-GK DICEQQPIGRACFRQFLSVSSPEYLAAAELLDELNCWNL-AEAE---AKEEARLNIINKFC-KADS-KS--FLAFLTEDEAEKCKAV-------------SEKDFEEVMMGQVKEATQKFLR-GQ WLCELQPIGRKLFLQFLMTSDPQRAAAAVFLQELDDWSF-AEDA---TKEKVRQRILAKFC-HPQS-QT--FLSYLTED-SNLCRTL-------------SVKSLDEETVQKITNATKDFLK-GK NVCEKQPIGKKLFHQFTA-TVPEYQVAVDFVNAVTDFQL-AENG---AKDNLRQNIVTNFL-KADS-KN--YLSFLSQELAAKCKDA-------------AEKDVEELL-ALAQSETNTFLM-GQ SVCERQPIGKKLFHTFTA-TVPEYQGAVEFVNEVTDFYL-AEDG---VKDNLRQNIVTNFL-KPDS-KN--YLSFLSQDLADKCKDA-------------TEKDFEELL-PMAQAEVRMFLA-GQ TVCEQQPIGKRLFHQLTA-TVPEYRVAVDFINEVSNFYL-AENG---DKENLRQKIVTDFL-KTDS-KN--YLSFLSQELGDKCKAA-------------TEKETEELL-PLAESEAKMFLK-EH NVCEQQPIGKMLFHQLTA-TVPEYQVAVDFINEVSNFYL-AENG---VKENLRQNIVTNFL-KPDS-KN--YLSFLSQDLVDKCKTA-------------AEKESEELL-ARAESEAKMFLK-GQ DVCEQQPIGKKFFHKFTE-TVPEYRIATDFLNEVNNIEL-AENG---ARENLLQNVVINFM-KADS-KN--YLSFLSEELASKCKSA-------------EDKDFEDLL-VMVKLEVRKFLM-GT AVCEVQPIGRHLFRKFLL-SKPEYSGIATFTDAVSKFEL-ADDD---GSRSGLLAVVVAFL-RAGS-VK--RLIFVSNELATKAESS-------------DANEQEAAI-SLARQEMRTFLS-GA AVCELQPIGKHFFRQFLE-SQPRYAGVTTFADAVTRFEL-AEDN---GSRGGLLGEALVFL-LADS-DT--KLAFVSDALAAKAASS-------------DAGEREAAI-PLARQEMRTFLS-GA SVCEAQPIGRRFFREFLD-SQPQYSSVAAFADAATKFEL-AEDDGGGGGRGAMLDEALAFM-RRDS-GE--RPAFVSESLAATAGSS-------------DAAEREAAI-ALAKREMRTFLS-GA GLCEEQPVGRRLFRLFAG-AVPEYRRAVEFAEEAERAQL-AEDG---AREGLAAATVAAFL-RADA-EG--FLAFLSAELAAEAVSA-------------SGPEAEELL-RRAGDETRRFLT-GP GLCEEQPVGRRLFRQFAG-GVPEYARAVEFLDEAEQVQL-SEDG---AREGLAGATVAAFL-RADS-ER--SLTFLSAELAAAVASA-------------GGREAEELV-RRAREEARLFLV-GQ GLCEEQPVGRRLFRQFAG-GVPEYAHAVEFLDEAEQVQL-SEDG---AREGLAAATVAAFL-RADS-ER--SLTFLSAELAAAAASA-------------GGQEVEELV-RRARGEARLFLV-GQ SECEEQPIGRRLFHSFCN-SVAEYSRALAFLDQVVELEL-AEDN---MQNALLCKIVADFL-QPDS-EN--SLSFLSDAMTSAASGA-------------SEVDMKELV-PRAQQETRQFLM-GK --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RVCNLEPIGRQLFRQFLD-TQKEFRPAGQLLQACEDMDT-SEDS---EKPEAAKKIVAEHMGDKNA-AS--FCPFLSAAAAQSARAN-------------GS------F-AEALAETLEHLR-AH RVCTLEPIGRQLFRQFLE-THNEFRPAGQLIQACEDMDM-SEES---EKPKRAQAILSAHMDDKNP-SS--FCAFLSAAAVEAARAR-------------GS------F-DETLAETAEYLR-SR --------------------------------------------------------------------------------MEAARAR-------------GS------F-AEALAETTEFLQ-SR WQCVEQPIGKRLFRQFLQ-ATPGLAAAGALWEELEAFEC-CEEG---ERSAAAANIRKRFF-TEGG-AE--HCAFLSAAATAPPADS-------------GAPDD---F-EPTRRELLAHLE-GT WQCVDQPVGKLLFRRFLE-GAPEFAAAGALWSEIEAFER-CEDA---EREASAKRLRSRFF-TPGG-SE--HCGFLSAAATAPPAGT-------------TMPGDA--F-AQARRELLAHLE-AA WQCVEQPIGKLLFQRFLE-GAPGLAAAGALWAELEAYER-CEEA---ERSGAAAALRRRFF-TPGG-AE--HCSFLSAAATATPTGE-------------APPTD---F-GQARRELLAHLE-AV SICLRQPIGKRLFQQFLE-DQEAFKAAGGLWKSMEEYAA-SEEN---ERTQKAQKTVNQFF-DSAS-KT--FCSFLEEKVVMRVKED-------------AKHGRADLF-KETEQHLLKHLE-DQ FICVRQPIGKRIFQQFLE-ETDEFKAAGGLWKSIEDYNT-SEEA---DRPKVAQKSVNKYF-DSAS-KE--FCSFLEEKAIMRVKED-------------AKNGRADLF-KEAEQHLLKYLE-AN FICVRQPIGKRLFQQFLE-ETEEFKAAGGLWKSIEEYNT-SEEP---ERPQKAQKSINKYF-DSAS-KE--FCSFLEEKAIMRVKED-------------GKHGHGDLF-KESEQHLLKHLE-EK NMCIKQPIGKRLLQQYLE-SEEAFKTAGTLWKDIEDYNT-AEEK---DRPQKAQKMVNKYY-DSAS-KT--FCGFLEEKAISRVKED-------------HKNCRGDLF-KESEQQLLRHLE-SR VMCIKMPIGKRLLQQYLE-SEDLLKTAGNLWKDIEDYST-AEEK---DRAQKAQKMVNKYY-DSAS-KN--FCSFLEEKAISRVKED-------------HKNCRADLF-KESEQQLLKHLE-NK SMCVKQPIGKRLFQQYLE-SEATHKNACELWKDMEEYNT-AQET---DRMQKAQKIVNKFY-ESQS-KN--FCGFLEEKAVTRVKED-------------HKNTRGDLF-KESEQLLLKHLE-AK SMCVRQPIGKRLFQQYLE-DDAVHKSAGELWKDIEDYNT-AQEK---DRLQKAQKIVNKYY-DSAS-KN--FCSFLEEKAIARVKED-------------HKNVRGDLF-KESEQQLLKHLE-AT SMCVRQPIGKRLFQQYLE-SEADYKNPVDLWKDIEDYIV-AQEN---DRAKKAQKIVNKYY-ESSS-KN--FCDFLGDKAVSRVKED-------------FKNIRGDLF-KESEQQLLVHLE-AK SICCQQPVGKRLFQQHLR-STEQFKMAAGLWQVMEDYNSRLEEA---EHIPLALTIVHQYY-DPDS-RD--FCSFLSEDAIARVHEV-------------ARLGKADLF-RESEQQLLSYLE-AQ SICCRQPIGKRLFCDFLR-ASTQFHMAASLWQDVEEYGT-MEES---GRVPKAQSMVNQYY-EPSS-RD--FCSFLDEKTVLRVKED-------------HPNGHAELF-RESEGQLLAYLE-AQ SICCRQPIGKKLFQEYLR-GSGHFQVAARLWQDMEDYST-MEEF---GRVAKAQSMVNKYY-ESGS-RE--FCSFLDEKTVLRVKED-------------QGNGHAQLF-RESEGQLLSYLE-SQ SVCLEQPIGKKLFQQFLQ-SAEKHLPALELWKDIEDYDT-ADND---LQPQKAQTILAQYL-DPQA-KL--FCSFLDEGIVAKFKEG-------------PVEIQDGLF-QPLLQATLAHLG-QA GMCLEQPIGKRLFQQFLR-THEQHGPALQLWKDIEDYDT-ADDA---LRPQKAQALRAAYL-EPQA-QL--FCSFLDAETVARARAG----------------AGDGLF-QPLLRAVLAHLG-QA NICSEQPIGKRLFQQFLK-SIEQHVPAVELWRDIEDYDT-ADND---FRLQKAQAILANYL-DPKA-KL--FCSFLEDGMVEKVKEK-------------HSSIPNGLF-QPLLQTTLEHLS-GA NICSEQPIGKRLFQQFLK-NEEQHVPAVELWRDIEDYDT-ADND---FRLQKAQAILAGYL-DPNA-KL--FCSFLEEGMIEKVKEK-------------HSSIPNGLF-QPLLQATLGYLS-EA NICVKQPIGKKLFQEFLE-SAE-FTHIVEIWNDIEDYDV-AEDE---DRLYKARGIINKYL-DSDS-KQ--FCHYLDEKAIIKVVQD-------------CNKVSNMLF-KQLHKSTLDYLK-EN NICVKQPIGKKLFQEFLG-TVEQFVPALEIWGDIEDYDV-AEDE---DRLQKAVGIINKYL-ETHS-KY--FCIYLDEKIIAPVVEE-------------CNKVPDGLF-KELLQSTLDYLK-EN SICCQQPIGKRLFQEYLE-TVPQYKPASKLWKDIEEYDT-AEDK---DRVQKASKIISRYM-ESDA-KQ--FCSFLQGSTISKVKEE-------------HQMAKDDLF-VESLEGTLAFLR-DL SICNQQPIGKRLFQEYLE-TVPEYKPASKVWKDIEEYDT-AEDK---DRVQKASKIISRYM-VSTA-KH--YCSFLQENTVTKVNEE-------------HQNAKDDLF-VKSLEETLAFLN-GV SICVEQPIGKRLFQEYLE-TANEYKGPCRLWRDVEEYDT-AEDK---DRTQKAAKILQRYM-EPSA-KH--FCPFLPENGITKVKER-------------HQEAADDLF-VEILACVFDFLK-EV TVCVEQPIGKRLFQEFLD-SINEYKGPSRLWKDIEEYNM-AEDE---DRAKKASKILSRYM-EPDA-KH--YCPFLPENGITKVKEK-------------HKEAGDELF-SETMDSVMDFLK-EV SVCVEQPIGKRLFREFLE-ANQEYHGACRLWKDIEDYDL-AEDS---ERAKKASKIVQRYM-DPSA-KH--FCPFLPEDIIAKVKEN-------------LEVVGDDLF-AAALAKTLDYLR-EA NICIKQPIGKKLFQQYLE-TVAAYKPAYELWKGIEDYDT-AEDE---DRLQIAQSIISKFL-ESGA-EF--FCSYLEEKSISRAKED-------------YKNVREDLF-KECKEATVKFLKEGK SICVKQPIGKKLFQQYLD-SVAEYRPANELWKAIEVYDT-AEDE---DRLEKARNIISKFL-ESGA-EL--FCTYLEEKSILRVKED-------------CENVREDLF-RECKEATMKFLRDDS SICIRQPIGKRLFQEYLD-TLADYKPACELWKNIEDYDT-AEDE---ERVQKAQYIISKYL-ETGA-EL--FCSYLDEKCILRVKED-------------CENVREDLF-KECKELTMEFLK-NT SICVKQPIGKRLFQQYLD-TVAEYKPAYELWKNIEDYDT-AEDE---ERIQKARDTISKFL-ESGA-EL--FCSHLEEKCILRVKED-------------CENVREDLF-KECKELTMKFLK-SS SICVKQPIGKRLFQEYLE-SVAEYKPANEIWKDIETYNT-IEDE---DRVQKAQNIISKYL-ESGT-KL--YCSFLEEKSILRVKED-------------YQNVRDDLF-AESEEATVKFLK-DA SICEDQPIGQRLFQLFCE-TQSELSICRECLEQMRNYEE-TLLE---DRRQVAQTVFDKYL-KERT-SNGVLPPTVTNEMIDAIEERLKAPPECDQEEL-----NQDLF-SECSSAITNYLS-GQ SICEKQPIGRELFRQFCK-KDDKLNRCICFLNKVEEFEV-TTDD---NRKDAA-QILSKFL-ESES-PD-YLGDIISSETISQCRETIQRYTPEEQESLQEETPSKDVF-NQCRSEVSKYLS-GE SVCEKQPIGRELFRQFCK-KDAKLNRCICFMNKV-------------------------------T-PD-YLGDIISSETISQCRENIQRITSEEQESLQEETPSKNVF-NQCRSEVSKYLS-GE SLCERQPIGRLLFREFCA-TRPELSRCVAFLDGVAEYEV-TPDD---KRKACGRQLTQNFL-SHTG-PD--LIPEVPRQLVTNCTQR-----------L-EQGPCKDLF-QELTRLTHEYLS-VA SLCDKQPIGRLLFRQFCE-TRPGLECYIQFLDSVAEYEV-TPDE---KLGEKGKEIMTKYL-TPKS-PV--FIAQVGQDLVSQTEEK-----------L-LQKPCKELF-SACAQSVHEYLR-GE SLCDKQPIGRRLFRQFCD-TKPTLKRHIEFLDAVAEYEV-ADDE---DRSDCGLSILDRFF-NDKL-AA--PLPEIPPDVVTECRLG-----------LKEENPSKKAF-EECTRVAHNYLR-GE ..130.......140.......150.......160.......170.......180.......190.......200.......210.......220.......230.......240.......250

161 161 160 155 158 158 158 158 158 159 159 158 158 156 156 157 176 158 158 158 158 158 161 161 162 153 162 159 157 164 166 24 190 178 190 160 157 157 160 160 160 160 160 161 160 160 160 157 161 161 160 161 161 161 161 161 160 162 162 161 161 161 168 168 101 156 156 157

GRK7_Human_NP-631948 GRK7_Cattle_NP-776757 GRK7_Opossum_XP-001364799 GRK7_Tasmanian_devil_XP-003766156 GRK7_Chicken_XP-426681 GRK7_Pigeon_XP-005505950 GRK7_Ground-tit_XP-005527115 GRK7_Anole_XP-003218299 GRK7_Gecko_XP-015268958 GRK7_Xenopus_NP-001120573 GRK7_Nanorana_XP-018415869 GRK7_Florida_gar_30401-2-1 GRK7_Bowfin_27257-5-2 GRK7a_Zebrafish_NP-001027011 GRK7a_Medaka_NP-001098131 GRK7b_Zebrafish_NP-001028262 GRK7b_Medaka_XP-004075863 GRK7_Reef_shark_15561-1-1 GRK7_Bamboo_shark_35291-1-1 GRK7_Bluespot_ray_37188-7-2 GRK7_Western_ray_39734-1-1 GRK7_Elephant_shark_XP-007891889 GRK7-1_M.mordax_40500-6-1 GRK7-1_G.australis_58623-2-1w1-2 GRK7-1_P.marinus_S4RD08 GRK7-2-Non-contig_G.australis_26295-2-1w27699-1-1 GRK7-2_P.marinus_S4RL90 GRK7-2_L.camtschaticum_JL9580 GRK7-2_E.cirrhatus_127887-1-1 GRK-Trimmed_C.intestinalis_XP-009860997 GRK-Partial_C.savignyi_H2YPC9 GRK1X_M.mordax_42166-5-1 GRK1X_G.australis_57773-1-1 GRK1X-Partial_L.camtschaticum_JL8961 GRK1B_Chicken_NP-990026 GRK1B_Ground-tit_XP-005533433 GRK1B_Golden_eagle_XP-011599401 GRK1B_Turtle_XP-006112219 GRK1B_Anole_XP-003228556 GRK1B_Bearded_dragon_XP-020665279 GRK1B_Spotted_gar_XP-006627541 GRK1B_Bowfin_26676-4-1 GRK1B_Pike_XP-010901292 GRK1B_Asian_bonytongue_XP-018581705 GRK1B_Zebrafish_NP-001017711 GRK1B_Bamboo_shark_26651-1-1 GRK1B_Bluespot_ray_4032-1-1 GRK1B_Western_ray_34447-1-1 GRK1A_Human_NP-002920 GRK1A_Cattle_NP-776598 GRK1A_Opossum_XP-001364656 GRK1A_Tasmanian_devil_XP-003765754 GRK1A_Xenopus_XP-002937018 GRK1A_Nanorana_XP-018428616 GRK1A_Florida_gar_24879-1-1 GRK1A_Bowfin_29529-1-1 GRK1A_Zebrafish_NP-001029353 GRK1A-1_Medaka_NP-001188419 GRK1A-2_Medaka_XP-004081538 GRK1A_Reef_shark_53758-1-1 GRK1A_Bamboo_shark_8046-1-1 GRK1A_Bluespot_ray_29212-2-1 GRK1A_Western_ray_41513-9-2 GRK1A_Elephant_shark_XP-007889799 GRK_C.intestinalis_XP-009859302 GRK_B.floridae_XP-019631688 GRK_B.floridae_XP-002608270 GRK6_Human_NP-002073 GRK5_Human_NP-005299 GRK4_Human_NP-892027

::* *:: *: :** . . ** * . ::* * *::.: : * : :* .**:::*:. PFKDFV-TSAFYDKFLQWKLFEMQPVSDKYFTEFRVLGKGGFGE----VCAVQVKNTGKMYACKKLDKKRLKKKGGEKMALLEKEILEKVSSPFIVSLAYAFESKTHLCLVMSLMNGGDLKFHIY PFREFL-ASPFYDKFLQWKVFEMQPVSDKYFEEFRVLGKGGFGE----VCAVQVKNTGKMYACKKLDKKRLKKKNGEKMALLEKEILERVSSPFIVSLAYAFESKSHLCLVMSLMNGGDLKFHIY PFQDFQ-ASPFFDKFLQWKAFEQQPITEKYFYEFRVLGKGGFGE----VCAVQVKNTGKMYACKKLDKKRLKKKKGEQMALLEKEILEKVNSPFIVTLAYAFESKSHLCLVMSLMNGGDLKFHIY PFQDFQ-ASPFFDRFLQWKAFEQQPITEKYFEEFRVLGKGGFGE----VCAVQVKNTGKMYACKKLDKKRLKKKRGEQMALLEKEILEKVNSPFIVTLAYAFESKSHLCLVMSLMNGGDLKYHIY PFQDFQ-TSPFYDKFLQWKVFEKQPVTDKYFYEFRVLGKGGFGE----VCAIQVKNTGKMYACKKLDKKRLKKKCGEKMALLEKEILEKVNSPFIVTLAYAYESKTHLCLVMSLMNGGDLKYHIY PFQDFQ-TSPLFDKFIQWKVFEKQPVTEKYFYEFRVLGKGGFGE----VCAIQVKNTGKMYACKKLDKKRLKKKGGEKMALLEKEILEKVNSPFIVTLAYAYESKSHLCLVMSLMNGGDLKYHIY PFQDFQ-NSPFYDKFIQWKVFEKQPVTEKYFSEFRVLGKGGFGE----VCAIQVKNTGKMYACKKLDKKRLKKKSGEKMALLEKEILEKVNSPFIVTLAYAYETKTHLCLVMSLMNGGDLKYHIY PFQDFQ-TSPFFDKFVQWKGFEKQPVNEKLFDEFRVLGKGGFGE----VCAIQARNTGKMYACKKLDKKRLKKKGGEKMALLEKEILEKVNSRFIVTLSYAYQSKAALCLVMSLMNGGDLKYHIY PFEEFQ-KSQYFEKFLQWKAFEKQPVSERHFEEFRVLGKGGFGE----VCAVQVKNTGKMYACKKLDKKRLKKKNGEKMALLEKQILERVNSRFIVSLAYAYQTKTDLCLVMSLMNGGDLRFHIY PFQDYQ-NSSFYDRFLQWKAFERQQITQKYFYEFRILGKGGFGE----VCAIQVKNTGQMYACKKLDKKRLKKKNGEKMALLEKEILEKVHSPFIVSLAYAYETKSHLCLVMSLMNGGDLKFHIY PFEAYQ-NSDYYDKFLQWKAFERQPITHKFFYEFRVLGKGGFGE----VCAIQVKNTGQMYACKKLDKKRLKKKNGEKMALLEKEILEKVHSPFIVSLAYAYESKTHLCLVMTLMNGGDLKYHIY AFDEYQ-NSPFFERFLRWKVFERQPITEKYFYEFRILGKGGFGE----VCAIQVKVSGQMYACKKLDKKRLKKKKGEKMALLEKEILEKVNSLFIVKLAYAYETKSHLCLVMSLMNGGDLKYHIY AFEEYQ-NSFFFEKFLQWKVFERQPITEKYFYEFRILGKGGFGE----VCAIQVKNTGQMYACKKLDKKRLKKKKGEKMALLEKEILEKVNSPFIVKLAYAYESKSHLCLVISLMNGGDLKYHIY PFTEYQ-ASPFFDKFLQWKEYEKQPISEKYFYEFRTLGKGGFGE----VCAVQVKNTGQMYACKKLCKKRLKKKHGEKMALLEKKILERVNSLFIVSLAYAYDTKTHLCLVMSLMNGGDLKYHIY PFTEYQ-GSQYFDKFLQWKEYEKQPISDKYFYEFRTLGKGGFGE----VCAVQVKNTGQMYACKKLCKKRLKKKGGEKMALLEKQILEKVNSLFLVNLAYAYDTKTHLCLVMTLMNGGDLKYHIY PFEEYQ-TSLFFDRFVQWKKFEKQPITDKYFYEFRTLGKGGFGE----VCGVQVKTTGQMYACKKLDKKRLKKKSGEKMALLEKKILEMVNSLFIVNLAYAFDTKTHLCLVMTLMSGGDLKYHIF PFLEYL-KSPHFYRFLQWKECERQKITDKYFYEFRTLGRGGFGE----VCVVQVKYTGHMYACKKLDKRRLKKKSGERLALQEKQILEKVNSPFIVNLAYAYESRTHLCLVMDLMSGGDLKFHIY PFQDFQ-NSPFFSKFLQWKTFERQPITEKYFYQFRVLGKGGFGE----VCAIQAKTSGKMYACKQLDKKRLKKKQGHEMALLEKEILEQTNSRFIVNLAYAYQSKDRLCLVMTLMNGGDLRFHIY PFQDFQ-NSPFFTKFLQWKTFERQPITEKYFYQFRVLGKGGFGE----VCAIQVKTTGKMYACKQLDKKRLKKKHGEEMALLEKEILEKANSPFIVSLAYAYETKNYLCLVMTLMNGGDLKFHIY AFQDFL-NSPFFEMFLQWKTFERQPITEKYFYQFRILGKGGFGE----VCAMQVKITGKMYACKQLDKKRLKKKHGEEMALLEKEILETVNSRFIVNLAYAYETKTHLCLVMTLMNGGDLKFHIY AFRDFL-NSPFFDKFLQWKTLERQPITEKYFYQFRVLGKGGFGE----VCAIQVKVTGKMYACKQLDKKRLKKKHGEEMALLEKEILEKANSRFIVNLAYAYETKSHLCLVMTLMNGGDLKFHIY PFQDFQ-NSPFFDKYTQWKVFERQPINEKYFYEFRVLGKGGFGE----VCAIQVKISGKMYACKKLDKKRLKKKNGEKMTLLEKEILEKTNSRFIVNLSYAFETKSHVCLVMTLMNGGDLKFHIY VFHEFL-QSAFYEKFLQWKMLEKKPVTEKTFYEFRTLGKGGFGE----VCAVQAKVSGKMYACKKLDKKRLKKKKGEKLVLVEKEVLEIVNSPFVVQLAYAYETKTHLCLTMSLMIGGDLRYHIY AFREFI-DSASYEKFLQWKMLEKKPVTDKTFYEFRMLGKGGFGE----VCAVQAKVSGKMYACKKLEKKRLKKKNGEKMALLEKEVLEIVSSPFVVQLAYAYETKTHLCLTMSLMNGGDLKYHIY PFRG-D-NFRYYRQCLAVKTDRRTEVMQKYFHPHLKGSKTPHSECTCFVCAMQAKMSGKMYACKKLDKKRLKKKNGEKMALLEKELLEMVNSAFVVQLAYAYETKTHLCLTMSLMNGGDLKYHIY PFQEFL-ASPFFEKLLQWKVLERQPVNERSFYEFRTLGKGGFGE----VCAIQSKLSGKMYACKKLDKKRLKKKRGEQMALLEKEILESVNSHFVVRLAYAYQTKTHLCLVMSLMNGGDLKYHIY PFREFL-ASPFFDRLLQWKVLERQPVSERSFYEFRTLGKGGFGE----VCAIQGKLSGKMYACKKLDKKRLKKKRGEQMALLEKEILESVNSPFVVRLAYAYQTKTHLCLVMSLMNGGDLKYHIY PFQEFL-ASPFFDRLLQWKVLERQPVNERSFYEFRTLGKGGFGE----VCAIQGKLSGKMYACKKLDKKRLKKKHGEQMALLEKEILESVNSPFVVRLAYAYQTKTHLCLVMSLMNGGDLKYHIY PFREYL-ESPFFQRFLQWKALEQQKITEKHFYEFRVLGKGGFGE----VCAIQVKLTRKMHACKKLNKKSLKKKKGQKMALLEKEILESVNSSMVVRLAYAFETKEHLCLVMTLMNGGDLKFHIY ------------------------------------LGKGGFGE----VYAYQVKVTGKMYACKKIDKRKMKKMHAQGVVMSEKITLEAVNSPFVVSLTYAYENKNHLNLVMTLMGAGDLRFHIY -----------------------------------------------------------MYACKKIDKKKMKKQHAESVVMSEKITLEAVNSPFVVSLTYAYENKNQLNLVMTLMGAGDLRFHIY ALAKYV-GSPHYLRYLQFKWLEMQPMGEEWFSDFRVLGKGGFGE----VCACQAKATGKLYANKKLNKKRLKKRGGYEGAMVEKLILEKVHSRFIVSLSYAFQTKQELCLVMTIMNGGDLRFHIY SLAEYA-ESPRYLRYLQFKWLEMQPMGEEWFSDFRVLGKGGFGE----VCACQAKATGKLYANKKLNKKRLKKRGGYEGAIVEKVILEKVHSRFIVSLSYAFQSKQELCLVMTIMNGGDLRFHIY SLAEYV-ESPRYLRYLQFKWLEMQPMGEEWFSDFRVLGKGGFGE----VCACQAKATGKLYANKKLNKKRLKKRGGYEGAIVEKVILEKVHSRFIVSLSYAFQSKLELCLVMTIMNGGDLRFHIY AWAPYL-SSPYFGRFTQFKWLEGQPVGADAFADFRVLGKGGFGK----VCACQRRATGKMYANKKLNKKRLKKRQGYEAAMVEKRILARVHSRFIVSLACAFQTKTDLCLVMTLMNGGDLRYHVY AWAPYR-ASPEFSRFVQFKWLEGQAVNADAFAEFRVLGKGGFGE----VCACQRRATGKMYANKRLNKKRLKKRKGYEAALVEKRILARVHSRFIVSLACAFQTKTDLCLVMTIMNGGDLRYHIY AWTPYR-ASPEFGRFVQFKWLEGQTVSAEAFVDFRVLGKGGFGE----VCACQRRTTGKMYANKRLNKKRLKKRQGYEAALVEKRILARVHSRFIVSLACAFQTKTDLCLVMTLMNGGDLRYHVY ALAKYK-DSLFFARFLQFKWLEGQSVTEEWFLDFRVLGKGGFGE----VCACQMRATGKMYANKKLNKKRLKKRNGYEGAIVEKRILAKVHSRFIVTLAYAFQTKVDLCLVMTLMNGGDLRYHVY TMAKYK-ESLHFGRFLQFKWLESQSVTDEWFMDFRVLGKGGFGE----VCACQMKATGKMYANKRLNKKRLKKRNGYEGAIVEKRILAKVHSRFIVTLAYAFQTKLDLCLVMTLMNGGDLRYHVY ALGKYK-ESLHFYRFLQFKWLESQSVTDEWFMDFRVLGKGGFGE----VCACQMKATGKMYANKRLNKKRLKKRKGYEGAIVEKRILAKVHSRFIVTLAYAFQTKTDLCLVMTLMNGGDLRYHVY ALEGFR-GSLYFLRFVQFKWLEAQAVTEEWFMDFRVLGKGGFGE----VHACQMKATGKMYANKKLNKKRLKKRKGYEGAIVEKRILAKVHSRFIVTLAYAFQTKTDLCLVMTIMNGGDLRYHIY ALEGFK-GSLYFLRYLQFKWLEAQSVTEEWFMDFRVLGKGGFGE----VHACQMKATGKMYANKKLNKKRLKKRKGYEGAIVEKRILAKVHSRFIVTLAYAFQTKNDLCLVMTIMNGGDLRFHVY ALEGFK-NSMYFLRYVQFKWLEAQPIDEEWFMDFRVLGKGGFGE----VHACQMKATGKLYANKKLDKKRLKKRKGYQGAIVEKRILAKVHSRFIVSLAYAFQTKTNLCLVMTIMNGGDLRFHMY AVQGFK-DSIFFLRYVQFKWLESQSVTEEWFMDFRVLGKGGFGE----VHACQMKATGKMYANKKLNKKRLKKRKGYDGAMVEKRILAKVHSRFIVTLAYAFQTKMDLCLVMTIMNGGDLRYHIY ALTGFK-DSMYFLRYVQFKWLESQPVAEDWFMDFRVLGKGGFGE----VHACQMKATGKMYANKKLNKKRLKKRKGYDGAIIEKRILAKVHSRFIVTLAYAFQTKTDLCLVMTIMNGGDLRFHMY AFEGYK-TSQFFQRFLQFKGLEGQPVGDEWFADLRVLGKGGFGE----VCACQMRATGKLYANKKLNKKRLKKRNGHEAVKIEKQILAKVHSRFIVTLAYAFQTKHELCLVMTLMNGGDLRYHIY AAESYK-ESTFFLRFLQFKWLEAQPIGEEWFTDFRVLGKGGFGE----VCACQMRATGKMYANKKLNKKRLKKRKGYEATMLEKQILAKVHNRFIVSLAYAFQTKYELCLVMTLMNGGDLRFHIY ATEGYK-ASAFFLRFLQFKWLEAQPVSEEWFMDFRVLGKGGFGE----VCACQMRATGKMYANKKLNKKRLKKRKGYEAAIVEKQSLAKVHNRFIVSLAYAFQTKYELCLVMTLMNGGDLRFHIY PFQEYL-GSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGKGGFGE----VSACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILMKVHSRFIVSLAYAFETKADLCLVMTIMNGGDIRYHIY PFQEFL-DSLYFLRFLQWKWLEAQPMGEDWFLDFRVLGRGGFGE----VFACQMKATGKLYACKKLNKKRLKKRKGYQGAMVEKKILAKVHSRFIVSLAYAFETKTDLCLVMTIMNGGDIRYHIY PFKEYL-ESLHFLRFLQWKWLEAQPIGEDWFLDFRVLGKGGFGE----VSGCQMKATGKMYACKKLNKKRLKKRKGYQGAMVEKKILEKVHSRFIVSLAYAFETKTDLCLVMTIMNGGDLRYHIY PFREYL-ESLYFMRFLQWKWLEAQPIGEDWFLDFRVLGKGGFGE----VSGCQMKASGKMYACKKLNKKRLKKRKGYQGAIVEKRILSKVHSRFIVSLAYAFETKTDLCLVMTIMNGGDLRYHIY AFAQYK-ESKYFSKFLQWKKLEAQPIGDDWFMDFRILGKGGFGE----VSATQMRATGKMYACKKLSKKRLKKRKGFEGAMVEKRILAKVHSRFIVSLAYAFQTKNDVCLVMTIMNGGDLRFHIY AFSQYK-EGKIFSRFLQWKWLEAQPVGEDWFMDFRILGKGGFGE----VSASQMRATGKMYACKKLNKKRLKKRKGFDGAMVEKRILAKVHSRFIVSLAYAYQTKNEVCLIMTIMNGGDLRFHIY PFTNYL-ETMYLKRFLQWKWLEAQPIGEDWFLDFRILGKGGFGE----VSATQMRATGKLYACKKLSKKRLKKRKGYEGAMVEKRILARVHSRFIVSLAYAFQTKTDLCLVMTIMNGGDLRYHIY PFSNYK-ETMYFKRFLQWKWLEAQPIGEDWFLDFRILGKGGFGE----VSATQMRGTGKLYACKKLNKKRLKKRKGYEGAMVEKRILARVHSRFIVSLAYAFQTKTDLCLVMTIMNGGDLRYHIY PFTFYL-ETMYFKRFLQWKWLEMQPVAEDWFLDFRVLGKGGFGE----VSACQMRATGKLYACKKLNKKRLKKRKGYEGAMVEKRILARVHSRFIVSLAYAFQTKTELCLVMTIMNGGDLRYHIY PYTFFL-ESMYLKRFLQWKWLEMQPIGEDWFLDFRVLGKGGFGE----VSACQMRATGKLYACKKLNKKRLKKRKGYEGAMVEKRILARVHSRFIVSLAYAFQSKTELCLVMTIMNGGDLRYHIY PYVFFL-ESMYLKRFLQWKWLEMQPMDADWFLDFRVLGKGGFGE----VSACQMKATGKLYACKKLNKKRLKKRKGFEGAMVEKIILEKVHSRFIVSLAYAFQTKEELCLVMTIMNGGDLKYHIY AFEHYK-ESMHFMRFLQWKWLEAQPVGEDWFLDFRVLGKGGFGE----VSACQMKATGKLYACKKLNKKRLKKRKGKQGAIVEKRILARVHSRFIVSLAYAFQTKADLCLVMTIMNGGDLRYHIY PFELYK-ESMYFMRFLQWKWLEAQPIGEDWFLEFRVLGKGGFGE----VSACQMKATGKLYACKKLNKKRLKKRKGNQGAMVEKRILARVHTRFIVSLAYAFQTKAELCLVMTIMNGGDLRYHIY PFEAYK-ESMYFMRFLQWKWLEAQPIGEDWFMEFRVLGKGGFGE----VSACQMKATGKLYACKKLNKKRLKKRKGNQGAMVEKRILARVHSRFIVSLAYAFQTKAELCLVMTIMNGGDLRYHIY PFEGYK-ESMYFMRFLQWKWLEAQPVGEDWFLEFRVLGKGGFGE----VSACQMKATGKLYACKKLNKKRLKKRKGNQGAMVEKRILARVHSRFIVSLAYAFQTKAELCLVMTIMNGGDLRYHIY PFENYK-ESMYFDRFLQWKWLEAQPIGEEWFLDFRVLGKGGFGE----VSACQMKATGKLYACKKLNKKRLKKRKGYEGTIVEKRILARVHSRFIVSLAYAFQTKEELCLVMTIMNGGDLRYHIY PFKEFC-DSIYFSRFLQWKWLERQPVTKNTFRQYRVLGKGGFGE----VCACQVRSSGKMYACKRLEKKRIKKRKGEAMALNEKQILESVNSRFVVSLGYAYETKDALNLVLTIMNGGDLKFHIH PFENFREDKIYFSRFLQWKYLERQPVTKNTFRQYRVLGKGGFGE----VCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKTILQKINSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIH PFENFREDKIYFSRFLQWKYLERQPVTKNTFRQYRVLGKGGFGE----VCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKTILQKINSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIH PFADYL-DSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGE----VCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIY PFHEYL-DSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGE----VCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIY PFEEYQ-ESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGE----VCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIY .......260.......270.......280.......290.......300.......310.......320.......330.......340.......350.......360.......370.....

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GRK7_Human_NP-631948 GRK7_Cattle_NP-776757 GRK7_Opossum_XP-001364799 GRK7_Tasmanian_devil_XP-003766156 GRK7_Chicken_XP-426681 GRK7_Pigeon_XP-005505950 GRK7_Ground-tit_XP-005527115 GRK7_Anole_XP-003218299 GRK7_Gecko_XP-015268958 GRK7_Xenopus_NP-001120573 GRK7_Nanorana_XP-018415869 GRK7_Florida_gar_30401-2-1 GRK7_Bowfin_27257-5-2 GRK7a_Zebrafish_NP-001027011 GRK7a_Medaka_NP-001098131 GRK7b_Zebrafish_NP-001028262 GRK7b_Medaka_XP-004075863 GRK7_Reef_shark_15561-1-1 GRK7_Bamboo_shark_35291-1-1 GRK7_Bluespot_ray_37188-7-2 GRK7_Western_ray_39734-1-1 GRK7_Elephant_shark_XP-007891889 GRK7-1_M.mordax_40500-6-1 GRK7-1_G.australis_58623-2-1w1-2 GRK7-1_P.marinus_S4RD08 GRK7-2-Non-contig_G.australis_26295-2-1w27699-1-1 GRK7-2_P.marinus_S4RL90 GRK7-2_L.camtschaticum_JL9580 GRK7-2_E.cirrhatus_127887-1-1 GRK-Trimmed_C.intestinalis_XP-009860997 GRK-Partial_C.savignyi_H2YPC9 GRK1X_M.mordax_42166-5-1 GRK1X_G.australis_57773-1-1 GRK1X-Partial_L.camtschaticum_JL8961 GRK1B_Chicken_NP-990026 GRK1B_Ground-tit_XP-005533433 GRK1B_Golden_eagle_XP-011599401 GRK1B_Turtle_XP-006112219 GRK1B_Anole_XP-003228556 GRK1B_Bearded_dragon_XP-020665279 GRK1B_Spotted_gar_XP-006627541 GRK1B_Bowfin_26676-4-1 GRK1B_Pike_XP-010901292 GRK1B_Asian_bonytongue_XP-018581705 GRK1B_Zebrafish_NP-001017711 GRK1B_Bamboo_shark_26651-1-1 GRK1B_Bluespot_ray_4032-1-1 GRK1B_Western_ray_34447-1-1 GRK1A_Human_NP-002920 GRK1A_Cattle_NP-776598 GRK1A_Opossum_XP-001364656 GRK1A_Tasmanian_devil_XP-003765754 GRK1A_Xenopus_XP-002937018 GRK1A_Nanorana_XP-018428616 GRK1A_Florida_gar_24879-1-1 GRK1A_Bowfin_29529-1-1 GRK1A_Zebrafish_NP-001029353 GRK1A-1_Medaka_NP-001188419 GRK1A-2_Medaka_XP-004081538 GRK1A_Reef_shark_53758-1-1 GRK1A_Bamboo_shark_8046-1-1 GRK1A_Bluespot_ray_29212-2-1 GRK1A_Western_ray_41513-9-2 GRK1A_Elephant_shark_XP-007889799 GRK_C.intestinalis_XP-009859302 GRK_B.floridae_XP-019631688 GRK_B.floridae_XP-002608270 GRK6_Human_NP-002073 GRK5_Human_NP-005299 GRK4_Human_NP-892027

:***:**:*:*** *: *::*:** : .** .:*.**:: * *:: * :.*: . ** NVG------T--RGLDMSRVIFYSAQIACGMLHLHELGIVYRDMKPENVLLDDLGNCRLSDLGLAVEMKGG-KPITQRAGTNGYMAPEILMEKVSYSYPVDWFAMGCSIYEMVAGRTPFK---DY SVG------E--PGLDMSRVIFYSAQITCGVLHLHSLGIVYRDMKPENVLLDDLGNCRLSDLGLAVQIQDG-KPITQRAGTNGYMAPEILMEKASYSYPVDWFAMGCSIYEMVAGRTPFR---DY NVG------E--RGLAINRTIFYSAQITCGILHLHSIGIVYRDMKPENVLLDDHGNCRLSDLGLAVQIKEG-KPITQRAGTHGYMAPEILKEEESYSYPVDWFAMGCSIYEMVAGRTPFK---DY HVG------E--RGLEINRIIFYSAQITCGILHLHSIGIVYRDMKPENVLLDDRGNCRLSDLGLAVQIKDG-KSITQRAGTNGYMAPEILKEEESYSYPVDWFAMGCTIYEMVAGRTPFK---DY NVG------Q--RGLELNRVIYYSAQITCGILHLHSIKIIYRDMKPENVLLDDNGNCRLSDLGLAVQVKEG-KSITQRAGTNGYMAPEILKE-ESYSYPVDWFAMGCSIYEMIAGRTPFK---NF NVG------E--KGLEMKRVIYYSAQITCGILHLHSIKIVYRDMKPENVLLDDNGNCRLSDLGLAVQVKEG-KSITQRAGTNGYMAPEILKE-EDYSYAVDWFAMGCSIYEMIAARTPFK---DF NVG------E--RGLEMKRIIFYSAQITCGILHLHSIKIVYRDMKPENVLLDDNGNCRLSDLGLAVQVKEG-KSITQRAGTNGYMAPEILKE-EDYSYPVDWFAMGCTIYEMVAGRTPFK---DY NVG------E--RGLEMNRVIFYSAQITCGILHLHSIQIVYRDMKPENVLLDDNGHCRLSDLGLAVEVKEG-KEIRQRAGTNGYMAPEILNE-EAYTYPVDWFAMGCSIYEMIAGRTPFK---DF STG------E--RGLKMDRVIYYSAQMTCGLLHLHSINVVYRDMKPDNVLLDEHGNCRLSDLGLAVKVKDD-KPITQRAGTNGYMAPEILKEDETYSYPADWFSLGCTIYEMVAARTPFR---DH NVG------E--KGIEMKRVIFYSAQICCGILHLHSLKIVYRDMKPENVLLDENGNCRLSDLGLAVKVKEG-KPITQRAGTNGYMAPEILTDE-DYSYPVDWFAMGCSIYEMVAAHTPFR---DP NVG------E--KGLEIKRVIFYTAQICCGILHLHSIKILYRDMKPENVLLDDNGNCRLSDLGLAVKVKEG-QPITTRAGTNGYMAPEILKDE-NYSYPVDWFAMGCSVYEMIAAQTPFK---DP NIG------E--RGIEMNRAIYYTAQITCGILHLHSINIVYRDMKPENVLLDDNGQCRLSDLGLAVEIKEG-KSINQKAGTNGYMAPEILKE-EDYTYSVDWFALGCSIYEMVAGRTPFK---DF NIG------E--RGIEMNRAIYYTAQITCGMLHLHSINIVYRDMKPENVLLDDNGQCRLSDLGLAVEVKEG-KTISQKAGTNGYMAPEILKE-ETYTYSVDWFALGCSIFEMVSGRTPFK---DY NIG------E--KGIEMDRIIYYTAQIATGILHLHDMDIVYRDMKPENVLLDSQGQCRLSDLGLAVEIAVG-KTISQKAGTGAYMAPEILNE-TPYRTSVDWWALGCSIYEMVAGYTPFKGPDAK NIGYDGKGVD--KGIEMKRIIHYTAQITTGILHLHDMDIIYRDMKPENVLLDSQGQCRLSDLGLAIEIAPG-KTVTQMAGTGAYMAPEILSK-TPYRTSVDWWALGCSIYEMVAGYTPFKGPESK HVG------E--VGIEMERIIHYTAQITSGILHLHSMDIVYRDMKPENVLLDCQGQCRLSDLGLAVELPNG-KTTTQKAGTKGYMAPEILKQ-EPYRTSVDWWALGCSIYEMVAGRVPFR---DH ELG------E--RGIRMERVVYYTAQIISGILHLHSMDIVYRDMKPENVLLDGKGQCRVSDLGLAVELPNG-KMICQRAGTTGYMAPEVLKQ-EYYRTSVDWWALGCSIYEMVAARLPFR---DF NIG------E--KGIEMKRTIFYTAQITCGILHLHSLNIVYRDMKPENVLLDDDGNCRISDLGLAAKIKEG-KGINHKAGTNGYMAPEILKD-EEYTYSVDWFALGCSIYEMIAARTPFK---EH NIG------E--RGIEMKRTIFYAAQITCGILHLHSIKIVYRDMKPENVLLDDNGNCRLSDLGLAVKVKEG-KSITRIAGTNGYMAPEILKE-ENYKYPVDWFALGCSIYEMIAGRTPFK---DY NIG------E--KGIEMNRAIFYTAQITCGILHLHAQNIVYRDMKPENVLLDDNGNCRLSDLGLAVRIKEG-KAINHKAGTNGYMAPEILKQ-EKYTYSVDWFALGCSIYEMIAGRTPFK---DY NIG------E--KGLEMNRAMFYTAQITCGILHLHSQNIVYRDMKPENVLLDDNGNCRLSDLGLAVRIKEG-IAINHKAGTNGYMAPEILKE-EKYTYSVDWFALGCSIYEMIAGRTPFK---DY NIG------D--RGIEMSRVQFYTAQITCGLLHLHSINIVYRDMKPENVLLDENGNCRLSDLGLAVEIING-KKTNQRAGTNGYMAPEILKE-ENYTYSVDWFALGCSIYEMIAGRTPFK---DF QMG------T--PGLEKDRVLFYAAQITCGLQHLHTLRVIYRDMKPENVLLDDAGHCRLSDMGLAVLLKNDSKKTTQRAGTNGYMAPEVINE-ERYTYSCDWFSLGCTIYEMIAGRTPFK---DY EIG------E--PGLETDRVLFYAAQITCGMQHLHALRIVYRDMKPENVLLDDAGHCRLSDLGLAVLLKSDSKKITQRAGTNGYMAPEVINE-EGYTYSCDWFSLGCTIYEMTAGRTPFK---DY EMG------E--LGLEMERVLFYAAQITCGMQHLHALRIIYRDMKPENVLLDEAGHCRLSDLGLAVLLKDDGMKITERAGTNGYMAPEVING-EGYTYSCDWFSLGCTIYEMIAGRTPFK---GH EXX------X--XXXXXXXXXXXXXXXXXXXXXXXXXXXVYRDMKPENVLLDERGHCRLSDLGLAVVLGGGQETICQRAGTNGYMSPEVVLE-QPYSFDADWFATGCTVYEMICGRAPFK---DY EMG------E--RGLSPARTLFYAAQITCGLQHLHTARIVYRDMKPENVLLDERGHCRLSDLGLAVVLADGQETIRQRAGTNGYMSPEVVLE-EPYAFDADWFATGCTVYEMICGRAPFK---DY EMG------E--RGLSPARTLFYAAQITCGLQHLHAARIVYRDMKPENVLLDERGHCRLSDLGLAVVLADGQETIRQRAGTNGYMSPEVVLE-EPYAFDADWFATGCTVYEMICGRAPFK---DY NVG--------KRGLSMDRVLFYTAQITCGLHHLHRLSIIYRDMKPENVLLDERGNCRLSDLGLAVVLRNADDKITQRAGTNGYMSPEVIKE-EPYSYDADWFALGCTIHEMISGNTPFR---NF ETNEDG----KKRGLTPERTKFYSAQIILGLDHLHRENILYRDMKPENVLLDDKGNVRLADLGLVCILGEG-EKTKGRAGTIGFMPPEMLQS-KQYSYGVDWFSLGCTIFEMIEARGPFV---SETNNDG----KKKGLSSERAKFYTAQIILGLDHLHRENILYRDMKPENVLLDDGGNVRLADLGLVCILKDG-EQVKGRAGTIGFMPPEMLQS-KHYSYGADWFSLGCTIFEMVEAKGPFV---SDVD------EANPGFTEARTLFYSAQIVCGLEHLHQHRIIYRDLKPENVLLDDMGHVRLSDMGLATELQEGEDKTKGYAGTPGFMAPELVKG-ELYDYSVDYFTLGVTIFEMIQAKGPFR---YR DVD------DANQGFSEARTLFYSAQIVCGLEHLHQHRIVYRDLKPENVLLDDMGHVRLSDLGLAIELKEGQDKTKGYAGTPGFMAPELLKG-EPYDYSVDYFTLGVTIFEMIQAKGPFR---YR DVD------DANQGFPEARSLFYAAQIVCGLEHLHQHRIVYRDLKPENVLLDDMGHVRLSDLGLAIELKEGQDKTKGYAGTPGFMAPELLKG-EPYDYSVDYFTLGVTIYEMIQAKGPFR---YR NVD------EENPGFPEPRAVFYTAQILLGLEHLHQHRIVYRDLKPENVLLDDAGHVRLSDMGLAVELKDGQNKTRGYAGTPGFMAPEVLRD-EEYDMAVDYFTLGVTLYEMLDAKGPFR---RR NVD------EENPGFAEPRAVFYTAQILLGLEHLHQHRIVYRDLKPENVLLDDAGHVRLSDMGLAVELKDGESKTRGYAGTPGFMAPELLKN-EDYDWSVDYFTLGVTVYEMLEAKGPFR---RR NVD------EDNPGFPEPRAVFYTAQILLGLEHLHQHRIVYRDLKPENVLLDDAGHVRLSDMGLAVELKEGQTKTRGYAGTPGFMAPEVLRD-EEYDWAVDYFTLGVTLYEMLEAKGPFR---RR NVD------EKNPGFPESRAVFYTAQIICGLEHLHQNRIIYRDLKPENVLLDDAGHVRLSDLGLAVELPEGKDKTKGYAGTPGFMAPELLQN-QEYDWSVDYFTLGVTLYEMIEAKGPFR---SR NVD------EKNPGLSESRTIFYTAQIICGLEHLHQNRIIYRDLKPENVLLDDAGHVRLSDLGLAVELPEGKDKTKGYAGTPGFMAPELLKG-EEYDSSVDYFTLGVTIFEMIAAKGPFR---SR NVD------EKNPGFSEARAVFYTAQIICGLEHLHQNRIIYRDLKPENVLLDDAGHVRLSDLGLAVELPDGKDKTKGYAGTPGFMAPELLKG-EEYDSSVDYFTLGVTLYEMVAAKGPFR---SR NVD------EKNPGLNEERTCFYTAQVICGLEHLHQNRIIYRDLKPENVLLDDAGHVRLSDLGLSVELEEGHDKTKGYAGTPGFMAPELLKN-EEYDYSVDYFTLGVTVFEMIAAKGPFR---SR NVD------EKNPGFNEERTCFYGAQIICGLEHLHQNRIIYRDLKPENVLLDDAGHVRISDLGLAIELKDGLNKTKGYAGTPGFMSPELLKN-EEYDYCVDYFTLGVTLYELVAAKGPFR---SR NVD------EKNPGFNETRACFYIAQIILGLEHLHQHRIVYRDLKPENVLLDDAGHVRLSDLGLAVELPAGTDKTKGYAGTPGFMAPELIEN-KEYDYTVDYFTLGVTLYEMISAKGPFR---TR NVD------EKNPGFNETRACYYTAQIICGLEHLHQHRIIYRDLKPENVLLDDAGHVRISDLGLAVELKPGKDKTKGYAGTPGFMAPELLQN-KEYDYSVDYFTLGVTLYEMIAAKGPFR---SR NVD------EKNPGFNETRACYYTAQIICGLEHLHQHRIVYRDLKPENVLLDDAGHVRLSDLGLAVELPPGKDKTQGYAGTPGFMAPELLQK-KEYDYTVDYFTLGVTLYEMVAAKGPFR---CR NVN------EKMPGFEEARACFYSAQILCGLQHLHQNRIIYRDLKPENVLLDDNGHVRISDLGLATVLKEGCSHTRGYAGTPGFMAPEMLKG-ECYDFCVDYFTLGVTLYEMVTAKGPFR---SR NVD------EKNPGFQESRAQFYTAQIICGLEHLHQHRIVYRDLKPENVLLDDGGHVRISDLGLATVLKEDREYTKGYAGTPGFMAPEMLKG-EPYSFSVDYFTLGVTLYEMVAAKGPFR---TR SVD------EKNPGFEESRARFYTAQIICGLEHLHQHRIIYRDLKPENVLLDDGGHVRISDLGLATVLKEGREQTKGYAGTPGFMAPEMLKG-EHYSFSVDYFTLGVTLYEMVAAKGPFR---VR NVN------EENPGFPEPRALFYTAQIICGLEHLHQRRIVYRDLKPENVLLDNDGNVRISDLGLAVELLDGQSKTKGYAGTPGFMAPELLQG-EEYDFSVDYFALGVTLYEMIAARGPFR---AR NVD------EDNPGFQEPRAIFYTAQIVSGLEHLHQRNIIYRDLKPENVLLDDDGNVRISDLGLAVELKAGQTKTKGYAGTPGFMAPELLLG-EEYDFSVDYFALGVTLYEMIAARGPFR---AR NVN------EENPGFEEPRAVFYIAQIVCGMEHLHQKRIVYRDLKPENVLLDNDGNVRISDLGLAVELKEGQTKTKGYAGTPGFMAPELLRS-EEYDFSVDYFALGVTLYEMIAATGPFR---AR NVN------EENPGFEEARAIFYIAQIICGMEHLHQKRIVYRDLKPENVLLDNDGNVRISDLGLAVELKEGETKTKGYAGTPGFMAPELLQS-EEYDFSVDYFALGVTLYEMIAARGPFR---AR NMD------EENPGIDEHRACFYTAQIISGLEHLHQNRIIYRDLKPENVLLDNDGNVRISDLGLAVELKEKKSKAKGYAGTPGFMAPELLQG-EEYDYAVDYFSLGVTLYEMIAARGPFR---CR NVD------EENPGLDEPRACFYTAQIICGMEHLHQNRIIYRDLKPENVLLDNDGNVRISDLGLAVELKEKKNKTKGYAGTPGFMAPELLNG-EEYDYSVDYFSLGVTLYEMIAARGPFR---CR NVD------ENNPGFEELRACFYTAQIICGMEHLHQKRIIYRDLKPENVLLDNDGNVRISDLGLAVELKDDQTKIKGYAGTPGFMAPELLKG-EEYDYSVDYFTLGVTLYEMVAAKGPFR---TR NVD------ENNPGFEEARACFYTAQIICGMEHLHQKRIIYRDLKPENVLLDNDGNVRISDLGLAVELKDDQTKIKGYAGTPGFMAPELLKG-EEYDYSVDYFTLGVTLYEMIEAKGPFR---TR NVD------ENNPGFSEPRACYYAAQIIQGLEHLHQKKIIYRDLKPENVLLDNEGNVRISDLGLAVELADGQEKTKGYAGTPGFMAPELLKG-EEYDYSVDYFTLGVTLYEFIAAKGPFR---TR NVD------ENNPGFDEPRACYYAAQIIQGLEHLHQKRIIYRDLKPENVLLDNEGHVRISDLGLAVELADDQFKIKGYAGTPGFMAPELLNG-EEYDYCVDYFTLGVTLYEFLAAKGPFR---TR LVD------ENNPGFDEPRACFYIAQIIQGLEHLHQKNIIYRDLKPENVLLDNDGNVRISDLGLAVELKEGKTKTKGYAGTPGYMAPEMLKG-DKYDSSVDYFTLGVTLYEFMAAKNPFR---NR NVD------ENNPGFDEKRACFYTAQIVCGMEHLHQSRIIYRDLKPENVLLDNEGHVRISDLGLAVELVDDQTKIKGYAGTPGFMAPELLKG-LEYDYSVDYFTLGVTLYEMIAAKGPFR---YR NVD------ENNPGFSEERACFYTAQIVCGMEHLHQSRIIYRDLKPENVLLDNEGHVRISDLGLAVELNDDQTKIKGYAGTPGFMAPELLKG-LEYDYSVDYFTLGVTLYEMIAAKGPFR---YR NVD------ENNPGFEEERARFYTAQIICGLEHLHQSRIIYRDLKPENVLLDNDGHVRISDLGLAVELKPDENKTKGYAGTPGFMAPELLGG-VEYGYSVDYFTLGVTLFEMIAAKGPFR---YR NVD------ENNPGFDEERARFYTAQIVCGLEHLHQCRIIYRDLKPENVLLDNDGHVRISDLGLAVELKPNETKTKGYAGTPGFMAPELLEG-LEYDYSVDYFTLGVTLYEMIAAKGPFR---YR NVD------ENNPGFGEDRACFYAAQIVCGMEHLHQNKIVYRDLKPENVLLDNEGHVRISDLGLAVELGENQTKTKGYAGTPGFMAPELLKS-EEYDYSVDYFTLGVTVYEMIAAKGPFR---TR NMG------A--PGFSIHRAIFYSAEITCGLHDLHKQRIVYRDLKPENILLDDHGHIRISDLGLAVRIPSG-EKIKGRVGTVGYMAPEVVRN-ERYEMSPDWFGLGCIIYEMIAGQAPFR---KR SMS------D-QPGFAESRAEFYAAEVLCGLQHLHQERIVYRDLKPENILLDDHGHVRISDLGLAVEIPEG-EMIRGRVGTVGYMAPEVVKN-EKYMFSPDWWGLGCLIYEMIEGKAPFR---AR SMS------D-PPGFAESRAEFYAAEVLCGLQHLHQERIVYRDLKPENILLDDHGHVRISDLGLAVEIPEG-EMIRGRVGTVGYMAPEVVKN-EKYMFSPDWWGLGCLIYEMIEGKAPFR---AR HMG------Q--AGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEG-QTIKGRVGTVGYMAPEVVKN-ERYTFSPDWWALGCLLYEMIAGQSPFQ---QR NMG------N--PGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEG-DLIRGRVGTVGYMAPEVLNN-QRYGLSPDYWGLGCLIYEMIEGQSPFR---GR NLG------N--PGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEG-QRVRGRVGTVGYMAPEVVNN-EKYTFSPDWWGLGCLIYEMIQGHSPFK---KY ..380.......390.......400.......410.......420.......430.......440.......450.......460.......470.......480.......490.......500

394 394 393 388 390 390 390 390 391 391 391 390 390 391 397 389 408 390 390 390 390 390 394 394 398 386 395 392 390 200 181 399 401 259 425 413 425 395 392 392 395 395 395 395 395 396 395 395 395 392 396 396 395 396 396 396 396 396 395 397 397 396 396 396 400 402 335 388 388 389

GRK7_Human_NP-631948 GRK7_Cattle_NP-776757 GRK7_Opossum_XP-001364799 GRK7_Tasmanian_devil_XP-003766156 GRK7_Chicken_XP-426681 GRK7_Pigeon_XP-005505950 GRK7_Ground-tit_XP-005527115 GRK7_Anole_XP-003218299 GRK7_Gecko_XP-015268958 GRK7_Xenopus_NP-001120573 GRK7_Nanorana_XP-018415869 GRK7_Florida_gar_30401-2-1 GRK7_Bowfin_27257-5-2 GRK7a_Zebrafish_NP-001027011 GRK7a_Medaka_NP-001098131 GRK7b_Zebrafish_NP-001028262 GRK7b_Medaka_XP-004075863 GRK7_Reef_shark_15561-1-1 GRK7_Bamboo_shark_35291-1-1 GRK7_Bluespot_ray_37188-7-2 GRK7_Western_ray_39734-1-1 GRK7_Elephant_shark_XP-007891889 GRK7-1_M.mordax_40500-6-1 GRK7-1_G.australis_58623-2-1w1-2 GRK7-1_P.marinus_S4RD08 GRK7-2-Non-contig_G.australis_26295-2-1w27699-1-1 GRK7-2_P.marinus_S4RL90 GRK7-2_L.camtschaticum_JL9580 GRK7-2_E.cirrhatus_127887-1-1 GRK-Trimmed_C.intestinalis_XP-009860997 GRK-Partial_C.savignyi_H2YPC9 GRK1X_M.mordax_42166-5-1 GRK1X_G.australis_57773-1-1 GRK1X-Partial_L.camtschaticum_JL8961 GRK1B_Chicken_NP-990026 GRK1B_Ground-tit_XP-005533433 GRK1B_Golden_eagle_XP-011599401 GRK1B_Turtle_XP-006112219 GRK1B_Anole_XP-003228556 GRK1B_Bearded_dragon_XP-020665279 GRK1B_Spotted_gar_XP-006627541 GRK1B_Bowfin_26676-4-1 GRK1B_Pike_XP-010901292 GRK1B_Asian_bonytongue_XP-018581705 GRK1B_Zebrafish_NP-001017711 GRK1B_Bamboo_shark_26651-1-1 GRK1B_Bluespot_ray_4032-1-1 GRK1B_Western_ray_34447-1-1 GRK1A_Human_NP-002920 GRK1A_Cattle_NP-776598 GRK1A_Opossum_XP-001364656 GRK1A_Tasmanian_devil_XP-003765754 GRK1A_Xenopus_XP-002937018 GRK1A_Nanorana_XP-018428616 GRK1A_Florida_gar_24879-1-1 GRK1A_Bowfin_29529-1-1 GRK1A_Zebrafish_NP-001029353 GRK1A-1_Medaka_NP-001188419 GRK1A-2_Medaka_XP-004081538 GRK1A_Reef_shark_53758-1-1 GRK1A_Bamboo_shark_8046-1-1 GRK1A_Bluespot_ray_29212-2-1 GRK1A_Western_ray_41513-9-2 GRK1A_Elephant_shark_XP-007889799 GRK_C.intestinalis_XP-009859302 GRK_B.floridae_XP-019631688 GRK_B.floridae_XP-002608270 GRK6_Human_NP-002073 GRK5_Human_NP-005299 GRK4_Human_NP-892027

* :: : *:. : KEKVSKEDLKQRTLQDEVKFQHDNFTEEAK----DICRLFLAKKPEQRLGS----REKS--DDPRKHHFFKTINFPRLEAGLI--------EPPFVPDPSVVYAKDIAEIDDFSEVRGVEFDDKD KEKVSKEDLKQRTLKEEVRFQHSNFTEEAK----DICRLFLAKTPEQRLGS----REKS--DDPRKHHFFKTINFPRLEAGLV--------EPPFVPDPSVVYAKDINEIDDFSEVRGVEFDDND KEKVSKEDLKQRTLEQEVKFQHEGFTEEAK----DICRLFLAKKAEQRLGS----RQEG--DDPRKHAFFQTINFPRLEAGLV--------EPPFVPDPSVVYAKDIADIEDFSEIKGVEFDDRD MEKVSKEDLKQRTLKEEVIFQHSGFTEEAK----DVCRLFLAKRAEQRLGS----REVG--DDPRKHSFFKTINFPRLEAGLV--------EPPFVPDPNVVYAKDVGDIEDFSEVRGIEFDEKD KEKVDKDEVKRRTLEDEVKFEHANFTEETK----DICRLFLAKKPENRLGS----RSED--DDPRKHSFFKTINFHRLEANLI--------DPPFVPDPSVVYAKDVADIADFSEIRGIEFDDKD KEKVGKDEVKRRTLEDEVKFEHANFTEEAK----DICRLFLAKKTENRLGS----RNEG--DDPRKHSFFKTINFHRLEANLI--------DPPFVPDPSVVYAKDVADIADFSEIRGIEFDDKD KEKVNKDEVRRRTLEDEVKFEHASFTEEAK----DICKLFLAKKKEDRLGS----RNED--DDPRKHSFFKTINFHRLEANLI--------DPPFVPDPSVVYAKDVADIADFSEIRGIEFDDKD KEKVNKDEVKKRTLEDDPKYEHKDFDEPTK----NICNLFLAKKPENRLGS----RSND--DDPRKHPFFKSINFHRLEAGLI--------DPPFVPDPSVVYAKDVADIADFSEVRGIEFDDKD KEKISKEELKRRTIEDEVKFEHSGFDEAAK----DICRMFLAKKVENRLGS----RSAN--DDPRKHHFFKSINFRRLEAGVT--------DPPFVPDPSVVYAKDVADIDDFSEIRGIEFNDKD KDKTSKEELKRRTLEDEVGFQHQNFTEEAK----DICRLFLAKKPQNRLGS----RTND--DDPRKHSFFKSINFQRLEAGMV--------DPPFVPDPSVVYAKDISDIADFSEIKGIEFDDKD KEKT-KDEVKRKTLEDEVVFHHPNFTAEAK----DICKLFLAKKPENRLGS----RTND--DDPRKHSFFSTINFNRLEAGMV--------DPPFVPDPSVVYAKDIADIADFSEIRGIDFDDKD KEKMNKDEVKRRTLEEEVKYDHPKFDELTK----DICKQFLMKKVEDRLGC----RGTH--DDPRKHAWFKSIVFQRLEAGLI--------DPPFVPDPSVVYAKEIGDIADFSEIRGIEFDDKD KEKVNKDEVKRRTLEDEVKFEHPKFDEPTK----DICKQFLMKKVEDRLGC----RGAS--DDPRKHHWFKSIVFQRLEAGLI--------DPPFVPDPSVVYAKEIGDIDDFSEVRGIEFDDRD KEKVEKEEVQRRILNEEPKFEHKNFDAATI----DIIKQFLKKKIDERLGC----K--N--DDPRKHEWFKSINFARLEAGLI--------DPPWVPKPNVVYAKDTGDIAEFSEIKGIEFDAKD KEKVEKEEVQRRILNEEPKWEHKCFDAPTK----DVIQQFLKKKIDERLGM----RNNM--EDPRKHEWFKSINFPRLEAGLV--------DPPWVPKPNVVYAKDTGDIAEFSEIKGIEFDAKD KEKVAKEELLRRTLEDEVKFEHRNFDAPSK----DIISLFLKRNIEDRLGS-------N--DDPRKHEFFKSINFPRLEAGLI--------PPPWEPKANVVYAKDTGDIREFSDVKGVKFDAND REKVQNTEVTRRTLEDDCKFEDKRFDSPTK----EIIVQFLRKKVAHRLGC----RK-G--DDPRNHSFFKNINFHRLEAGLV--------EPPWVPKANVVYAKNIEDCRDNSDVRDVELNCKD KEKVNKDEVKRRTLEDEVKYENPKFTEDSK----DICSLFLAKNIEERLGC----RGEN--DDPRNHPFFKSINFQRLQAGLV--------DPPFVPDPSTVYCKDIGDIAEFSEIKGIEIVEQD KEKVSKDEVKRRTLEDEVNYENPKFTEESK----DICKLFLAKGSEERLGC----RGEN--DDPRNHPFFKSINFQRLEAGLT--------EPPYVPDPSTVYCKDIGDIADFSEIKGVEITDQD KEKVSKDEVTRRTLEDEVKYEHPKFTEESK----DICRLLLAKNSEERLGC----RGEN--DDLRKHPFFKSINFQRLEAGLI--------KPPFVPDPSTVYCKDLGDIADFSEVQGIEIIDKD KEKVSKDEVKRRTLEDEVKYEHPNFTEESK----NICRLLLAKNSEERLGC----RGEN--DDPRKHPFFKSINFQRLEAGLI--------EPPFVPDPRTVYCKDLGDIADFSEVQGIDIIDKD KEKVSKDEVKRRTLEDEETYLHGNFTDESK----DICKLFLAKKIEDRLGC----NQEC--SDPRKHSFFKSINFQRLEAGLV--------DPPFVPNPATVYCKDLGDIADFSEVRGIDIDEKD KETVTKEELRRRTLEDNVAYFHRNFCADSK----SICDQLLAKKPANRLGC----RGDD--DNPRKHPYFQSINFHRLEAGLV--------NPPFVPDPGKVYAKDIGDIMEFSEIKGIEIDEKD KEKVAKEEVRRRTLEDNVAYFHRNFTNDSK----SICDQLLAKKPADRLGC----RGDD--DNPRKHPYFQSINFYRLEAGLV--------NPPFVPDPGKVYAKDIGDIMEFSEAKGIEIDGKD KEKVTKDEVRRRTLEDNVAYLHRNFCPNSK----NICDQLLAKKPANRLGC----RGDD--DNPRKHPYFQSINFFRLEAGLV--------NPPFVPDPGKVYAKDISDIMEFSEAKGIEINEKD REKVSKEEVRRRTLEDAVAFTHPAFTDESR----DLCALMLAKKAEERLGC----RGPD--DNPRRHDFFRTVNFQRLEAGLG--------EPPFVPDPGIVYAKDVGDIQDFSETKGVELDDKD REKVSKEEVRRRTLEDAVVFSHPAFTDQSR----DLCTLMLAKKAQERLGC----RGPD--DDPRRHDFFRTVNFQRLEAGLG--------DPPFVPDPGIVYAKDVGDIQDFSETKGVELDDKD REKVSKEEVRRRTLEDAVVFSHPAFTDQSR----DLCTLMLAKKAQERLGC----RGPD--DDPRRHDFFRTVNFQRLEAGLG--------DPPFVPDPGIVYAKDVGDIQDFSETKGVELDDKD HEQVSKEELRRRVLQDKVVFTKTAFTAEAQ----DLCTQLLAKRACDRLGS----RNPN--DDPRQHGFFHSINFQRLEAGLV--------SPPFVPDPDVVYAKDLCDIDEFSEVKGVELDEQD KGKNKRKHCVESTAEKTINE-KHKFKKTHEPSLVDLIDKLLVKKVEDRLAG----DEKS-AEAIKRHPYFADLNWERLEAGVI--------PPPFVPDPRQVYAKDVSDIRLGSEAKGVVLTKED KGKNRRKLCVESTAEKTIYE-KHKFKKTEDPLLIDLVDKLLVKDVANRLAG----DEKS-AEAIK-----------------------------------------------------------GEKVENKEVKRRILNDSVAYS-DRFSADCR----ETCEALLAKDPAKRLGF----RDGS-CNELKKHRFFAAMNWGRLQAGML--------EPPFVPDSKTVYAKDVNEVGAFSSVRGVTIVEED GEKVENKEVKRRILEDGATYS-DRFSAACR----DTCEALLAKDPATRLGF----RDGS-CNELKKHGFFATINWGRLQAGLL--------EPPFVPDSKTVYAKDVNEVGAFSSVRGVTIVEDD GDEVENKEVKRRILQDSASYS-ERFSADCR----ATCEALLAKDPAERLGF----RDGS-CGELKKHGLFASINWGRLQAGLL--------EPPFVPDSKTVYAKDVDEVGAFSSVRGVNLVEED GEKVEKKEVTRRTLHDSVSYS-ERFSAASR----SLCEGLLAKDPQKRLGF----RDGN-CAMLRSQPVFSALHWGSW-SGPP--------PPPFVPDPRRVYAKDLGDVGAFSTVRGVELDGAD GEKVENKEVTRRILNDPVTYS-EKFSAAAR----EACEGLLAKDPQTRLGF----RDND-CAQLKAHPFFKSINWGSW-WGLV--------PPPFVPDPRRVYAKDLGDVGAFSTVKGVELDAGD GEKVENKEVTRRILHDPVSYS-DRFSPAAR----AACEGLLAKDSSARLGF----REND-CAQLKAHPLFAAINWGRLEAGLV--------PPPFVPDPRRVYAKDLGDVGAFSSVRGVELDEGD GEKVENKEVTRRILHDPVQYS-EKFSPTCQ----AACEGLLAKDPAGRLGF----RDNQ-CAQLKALPLFQKMNWGRLEAGLL--------EPPFVPDPKTVYAKDIEEVGAFSTVKGVMLDDQD REKVENKEVTRRILNDPVTYPDDKFSADCK----ACLDALMAKDPANRLGF----KNNE-CSELKNHALFKPINWGRLEAGLI--------EPPFVPDPKVVYAKDIGDVGAFSTVKGVVLDDKD GEKVENKEVTRRILNDPVQYP-DTFSAECK----SCCEGLMAKDPAGRLGF----KNNA-CDELKSHALFQKMNWGRLEAGMV--------EPPFVPDPKVVYAKDIGDVGAFSTVKGVVLDDTD GEKVELKEVTRRILNDPVSYP-DVFGADCK----ALCERLMEKDPARRLGF----RNGE-CAELKQQPFFRGLNWGRLEAGLI--------DPPFVPDPKMVYAKDISDVGAFSTVKGVVLDDKD GEKVENKEVTRRILNDPVSYP-DKFSANLK----ALCEALMEKDPAKRLGF----KDNE-CMDLKNHVFFKTINWGRLEAGLL--------PPPFVPDPKMVYAKDIDDVGAFSTIKGVVLDEKD GEKVENTEVERRILNDAVVYT-DKFSKDCK----AICEGLMEKDPVKRLGF----KNNE-CAELKEQPFFKELNWGRLEAGLL--------PPPFVPNPNTVYAKDIDDVGAFSTIKGVVIDATD GEKVDNNEVTRRILSEPVSYP-DTFGKECK----DFCEGLMEKDPEKRLGF----KNNS-CDEMKNQPFFKELNWGRLEAGML--------TPPFVPDPKMVYAKDIDDVGAFSTIKGVVMDEKD GEQVDNNEVTRRILNDPVSYP-PTFSQELK----DLCEGLMEKDPEKRLGF----KNNE-CAELKNQSFFKELNWGRLEAGML--------PPPFVPDPKMVYAKDIDDVGAFSTVKGVVIDNKD GEKVEPQEVSRRTLNEPVDYP-SVFSQDCK----SLCEALMTKDPSERIGF----KLGS-CSEVKTHRFFQTLNWARLEAGLI--------EAPFTPDPKTVYAKDIADVGTFSSIKGVVITESD GEKVENQEVTRRILNDVVQYP-LSFSGECK----SLCEALMAKDVSQRLGF----KNGN-CSEVKNQPFFSTINWIRLEAGLV--------TPPFIPDPKTVYAKDIGDVGMFSSVKGVIITDSD GEKVENQEVTRRILNDAVKYP-PSFSADCE----ALCEALMAKDTSQRLGF----KDGH-CSEVKNQPFFKSINWGRLEAGLV--------TPPFVPDPKTVYAKDIGDVGMFSSVKGVVITDSD GEKVENKELKHRIISEPVKYP-DKFSQASK----DFCEALLEKDPEKRLGF----RDET-CDKLRAHPLFKDLNWRQLEAGML--------MPPFIPDSKTVYAKDIQDVGAFSTVKGVAFDKTD GEKVENKELKQRVLEQAVTYP-DKFSPASK----DFCEALLQKDPEKRLGF----RDGS-CDGLRTHPLFRDISWRQLEAGML--------TPPFVPDSRTVYAKNIQDVGAFSTVKGVAFEKAD GEKIENKELKQRIISEPVKYT-EKFSKASK----DFCEALLEKDPEKRLGF----KNGN-CDELRVNVLFKDVNWRQLEAGML--------IPPFIPDSRTVYAKNIQDVGAFSTVKGIVFDKAD GEKIENKELKQRIISDPVTYS-EKFSKASK----DFCEGLLEKNPEKRLGF----KNGN-CDELRANVLFKDINWRQLEAGTL--------EPPFIPDSRTVYAKNIQDVGAFSTVKGIVFDKTD GEKVENKELKNRILNDSVTYS-GKFSLDCK----SICEALLDKDPKKRMGF----KNGN-CNEIRVHLFFRGINWRKLEAGIL--------PPPFAPDSRIVYAKDIQDVGAFSTVKGVTLDESD GEKVEPKELKSRIINDAVTYT-EKFSDECK----SFCEALLEKDAKKRLGF----RNGN-CNDLRVVPFFREINWRKLEAGIL--------LPPFTPDSRTVYAKDIQDVGAFSTVKGVNIEEND GEKVENKEVKKRILNDPVVYT-EKFSEEAK----AFCEGLLAKEVENRLGL----KNGN-CDELRAHPFFNSINWRKLEAGIL--------PPPFVPDSKTVYAKDIQDVGAFSTVKGVTLEDND GEKVENKEVKKRILNDPVAYS-EKFSEEAK----SFCEALLAKEVDKRLGF----KNGN-CEELRVHPFFSSINWRKLEAGIL--------PPPFVPDSKTVYAKDIQDVGAFSTVKGVNLDEPD GEKVENKEVKKRILNDPVTYS-EKFSENAK----SICEGLLAKEVDKRLGF----KNGT-CDEIRTHPFFSQLNWRKLDAGIL--------PPPFVPDSKTVYAKDLDDVGAFSTVKGVCLEDDD GEKVENKVVKKRILNDPVTYP-EKFSENGK----SICEGLLCKEADKRLGF----KNGS-CDELRTHPFFSQINWRKLNAGIL--------PPPFVPDSKTVYAKDLDDVGAFSTVKGVQLEDVD GEKVEREEMKERMLTREVTYQ-DNFSEHAR----SLCDGLLARDVDKRMGF----KNGC-CDEIRAHPFFKDINWRKLNAGII--------QPPFVPDPKVVYAKSLDDVGAFSSVKGVTLEDPD GEHVENKEVKRRILEDPVTYC-EKFSEPCK----LFCEALLEKEPEKRLGF----KNGS-CDEIRSHPFFKNINWRKLEAGIL--------LPPFMPDSKTVYAKDIQDVGAFSTVKGINIEDSD GEHVENKEVKRRILNDPVTYC-NKFSEPSK----LFCEALLEKDPEKRLGF----KNEC-CDEIKAHAFFKSINWRKLEAGIL--------PPPFTPDSKTVYAKDIQDVGAFSTVKGITIEDAD GEHVENKELKRRILNDPVSYS-EKFSESSK----MFCEALLEKDPEKRIGF----RNDC-CDELKAHAFFKDINWRKLEAGIL--------PPPFIPDSRTVYAKDIQDVGAFSTVKGVTLEDSD GEHVENKEVKRRILNDPVSYC-EKFSEGSK----LFCEALLEKDPNKRIGF----RNEC-CEELKAHVFFKDINWRKLEAGIL--------SPPFTPDSRTVYAKDIQDVGAFSTVKGVTLEDLD GEHVENKEVKRRILNDPASYT-EKFSEPCK----AFCEALLEKDPNKRVGF----KNGN-CDELKAHSFFKDINWRKLNAGIL--------PPPFTPDSKTVYAKDIQDVGAFSTVKGVNFDDPD KEKVKREEVDRRVKEDTESYDDEKFTEEAK----SICSMLLKKDVKDRLGC----RNGSCASDVKAHPFFKNINFKRLETGKAPFGNPPFDKVPFELDPHAVYAKDVLDIEQFSTVKGVTIDGKD KEKVKREEVDRRVKEDTETY-SDKFSEHAK----TICSALLQKDPKLRLGCVEDAMGSR-ADDIKAHPFFK-LNFKRLEAGMV--------KPEFEPDPRAVYAKDVLDIEQFSTVKGVNLDQAD KEKVKREEVDRRVKEDTETY-SDKFSEHAK----SICSALLQKDPKLRLGCVDDAMGSR-ADDIKTHTFFK-LNFKRLEAGMV--------KPEFVPDPRAVYAKDVLDIEQFSTVKGVNLDQAD KKKIKREEVERLVKEVPEEY-SERFSPQAR----SLCSQLLCKDPAERLGC----RGGS-AREVKEHPLFKKLNFKRLGAGML--------EPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTD KEKVKREEVDRRVLETEEVY-SHKFSEEAK----SICKMLLTKDAKQRLGC----QEEG-AAEVKRHPFFRNMNFKRLEAGML--------DPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTD KEKVKWEEVDQRIKNDTEEY-SEKFSEDAK----SICRMLLTKNPSKRLGC----RGEG-AAGVKQHPVFKDINFRRLEANML--------EPPFCPDPHAVYCKDVLDIEQFSVVKGIYLDTAD .......510.......520.......530.......540.......550.......560.......570.......580.......590.......600.......610.......620.....

501 501 500 495 497 497 497 497 498 498 497 497 497 496 504 493 514 497 497 497 497 497 501 501 505 493 502 499 497 311 240 506 508 366 531 519 532 502 500 499 502 502 502 502 502 503 502 502 502 499 503 503 502 503 503 503 503 503 502 504 504 503 503 503 517 512 445 495 495 496

GRK7_Human_NP-631948 GRK7_Cattle_NP-776757 GRK7_Opossum_XP-001364799 GRK7_Tasmanian_devil_XP-003766156 GRK7_Chicken_XP-426681 GRK7_Pigeon_XP-005505950 GRK7_Ground-tit_XP-005527115 GRK7_Anole_XP-003218299 GRK7_Gecko_XP-015268958 GRK7_Xenopus_NP-001120573 GRK7_Nanorana_XP-018415869 GRK7_Florida_gar_30401-2-1 GRK7_Bowfin_27257-5-2 GRK7a_Zebrafish_NP-001027011 GRK7a_Medaka_NP-001098131 GRK7b_Zebrafish_NP-001028262 GRK7b_Medaka_XP-004075863 GRK7_Reef_shark_15561-1-1 GRK7_Bamboo_shark_35291-1-1 GRK7_Bluespot_ray_37188-7-2 GRK7_Western_ray_39734-1-1 GRK7_Elephant_shark_XP-007891889 GRK7-1_M.mordax_40500-6-1 GRK7-1_G.australis_58623-2-1w1-2 GRK7-1_P.marinus_S4RD08 GRK7-2-Non-contig_G.australis_26295-2-1w27699-1-1 GRK7-2_P.marinus_S4RL90 GRK7-2_L.camtschaticum_JL9580 GRK7-2_E.cirrhatus_127887-1-1 GRK-Trimmed_C.intestinalis_XP-009860997 GRK-Partial_C.savignyi_H2YPC9 GRK1X_M.mordax_42166-5-1 GRK1X_G.australis_57773-1-1 GRK1X-Partial_L.camtschaticum_JL8961 GRK1B_Chicken_NP-990026 GRK1B_Ground-tit_XP-005533433 GRK1B_Golden_eagle_XP-011599401 GRK1B_Turtle_XP-006112219 GRK1B_Anole_XP-003228556 GRK1B_Bearded_dragon_XP-020665279 GRK1B_Spotted_gar_XP-006627541 GRK1B_Bowfin_26676-4-1 GRK1B_Pike_XP-010901292 GRK1B_Asian_bonytongue_XP-018581705 GRK1B_Zebrafish_NP-001017711 GRK1B_Bamboo_shark_26651-1-1 GRK1B_Bluespot_ray_4032-1-1 GRK1B_Western_ray_34447-1-1 GRK1A_Human_NP-002920 GRK1A_Cattle_NP-776598 GRK1A_Opossum_XP-001364656 GRK1A_Tasmanian_devil_XP-003765754 GRK1A_Xenopus_XP-002937018 GRK1A_Nanorana_XP-018428616 GRK1A_Florida_gar_24879-1-1 GRK1A_Bowfin_29529-1-1 GRK1A_Zebrafish_NP-001029353 GRK1A-1_Medaka_NP-001188419 GRK1A-2_Medaka_XP-004081538 GRK1A_Reef_shark_53758-1-1 GRK1A_Bamboo_shark_8046-1-1 GRK1A_Bluespot_ray_29212-2-1 GRK1A_Western_ray_41513-9-2 GRK1A_Elephant_shark_XP-007889799 GRK_C.intestinalis_XP-009859302 GRK_B.floridae_XP-019631688 GRK_B.floridae_XP-002608270 GRK6_Human_NP-002073 GRK5_Human_NP-005299 GRK4_Human_NP-892027

KQFFKNFATGAVPIAWQEEIIETGLFEELNDPNRPTGC---EEGN-SS----------------KSGVCLLL---------------------------KQFFQRFATGAVPIAWQEEIIETGPFAELNDPNRPAGC---GEG--SS----------------RSGVCLLL---------------------------RKFFKKFATGAVPIAWQEEIIETGLFEELNDPHRLSGC---PNGSQSS----------------KSGVCVLL---------------------------RKFFKKFATGAIPIAWQEEIIETGLYEELNDPSRL-GC---VNDS-TS----------------KSGVCVVL---------------------------KKFFKKFATGAVSIPWQEEIIETGLFEELNDPNRVDSGG-YANGGE-A----------------KSGVCLLL---------------------------KKFFKKFATGAVPIAWQEEIIETGLFEELNDPNRVDSGG-YANGGE-A----------------KSGVCLLL---------------------------KKFFKKFATGAVPIPWQEEIIETGLFDELNDPNRVVSGG-YANGGE-A----------------KSGVCLLL---------------------------KKFFKKFATGAVPIPWQEEVIETGLFDELNNPHRVVGV-------E-S----------------KSGVCVLL---------------------------NQFFKKFSTGAVSVSWQNEVIETGLFAELNDPNRVIGAG--------G----------------KSAVCLLL---------------------------AKFFKRFATGAVPISWQKEIIDTGLFDELNDPSREASG---GGGNSGE----------------KSGVCSIL---------------------------AKFFKRFATGAIPVSWQQEIIDTGLFDELNDPNRQSSGK-YTYGDGEK----------------KSGVCSII---------------------------KKFFKRFATGAVSIPWQEEMIETGLFDELNDPSRKESAA-GINS-E-S----------------RSGVCALL---------------------------KKYFKRFATGAVPIPWQEEMIETGLFEELSDPNRKESVS-GGGSGE-S----------------KSGVCALL---------------------------DKFFKEFSTGAVSIAWQQEMIDTGLFDELSDPNRKESSG-GSDD-D-K----------------KSGTCTLL---------------------------DKFFKEFSTGAVPIQWQQEMIETGLFDELNDPNRKEGAG-GGDD-E-K----------------KSGTCALL---------------------------EKFFKEFSTGAVPIAWQQGMIDSGLFDELSDPNRKESAA-GLEDEEQQ----------------KSKSCTLL---------------------------EKFFKEFSTGAVPIRWQREIIDSGLFDELNNSQMNGNCH-KTV----W----------------ISRMCIIL---------------------------NQFYKKFATGAIAIPWQEEMVESGLFDELNDPARRASMA-NNGMANGSGE-------------AKSGVCAIL---------------------------KQFYKKFSTGAVTIPWQEEIIESGVFDDLNDPSRQEA----GDIANGSGE-------------AKSGVCMIL---------------------------KQFYKKFATGAVAIPWQEEMVECGLYEELSDHTRKPSKA--AVIQNGSDE-------------AKSGVCMIL---------------------------KQFYRKFATGAVNIPWQEEMIECGLYDELNDPTRKPSKA--GMLANGTEE-------------AKSGVCIIL---------------------------KKFYKKFATGSVPIPWQEEMIESGLFEELNDPARRESKA--GFSANGSDE-------------AKSGVCTIL---------------------------QKFYSQFSTGCIPTAWQEEMIETGIFDDLNDPNH---VE-QKPTKNGT----------------PSSTCVLL---------------------------QVFYSKFSTGCIPATWQEEMIETGLFEELNDPNRVVEVE-KTKTSNGS----------------KSSTCVLL---------------------------QMFYSKFSTGCIPATWQEEMIETGLFEELNDPNRVVDVE-KGHMSNGN----------------RSSTCILL---------------------------RGFFARFATGPVPLAWQEEMIET----------------------------------------------------------------------------RGFYARFATGPVPLAWQEEMIEMGLFEEVNDDGATEGDR--GDGVNGDVG-------------RKSGVCHLL---------------------------RGFYARFATGPVPLAWQEEMIEMGLFEEVNNDGDTEGGR--GDGVNGDVG-------------RKSGVCHLL---------------------------LKFHKEFATGCVPLTWQEEIIESGLFDDVESVQHRASLP-------GNSE-------------RRSGVCLIL---------------------------TDFHKKFSSGRVAIPWQQEMLETGLFEDV-LSRPNPVVP-VVDSKKSKSK-----------------VCALL------------------------------------------------------------------------------------------------------------------------------KDFYDSFSTGSNPISWQEEIIETGVFQELNLWPADGSLP-NDLCKDKLPTSEGASG--------RSSACTVL---------------------------KNFYDSFSTGSIPIPWQEEMIETGVFGELNLWPADGSLP-DDLCKDKTPSADGGSA--------RSSACTVL---------------------------KAFYDSFSTGSIPIPWQEEMIETGVFGELNLWPANGSLP-DDLCKDKVPTSDGAGA--------RSSTCTVL---------------------------AALCDAFASGTVSIPWQEELIETGLFDELNVWGGDGRLP-PDLDPNAEPGS-GGGT--------RSATCGVL---------------------------AALCDAFASGTVPIPWQEELIETGVFEELNVWGAPGTLP-PDLDPSAAGG--AGGG--------KSSTCRLL---------------------------AALCDTFASGTVPIPWQEELIETGVFEELNIWGPPGTLP-PDLDPASAP---GGGV--------RSGTCCLL---------------------------KAFYEEFSSGNIPIPWQEEMVETGVIRELNIWGPKGTIP-RDLDHTM-PAN-SVSS--------KSGTCLLL---------------------------KEFYDDFSSGNIPIPWQEEMIETGIFGELNVWGAKGTIP-KDLDRNA-PVS-STTT--------KSGTCLLL---------------------------KAFYDEFSTGNIPIPWQEEMIETGVFGELNIWGAKGTTP-RDLDRNARPSS-DATT--------KSGTCLLL---------------------------KEFYDEFATGNIPIPWQDEMIETGVFGELNVWGQKGSTP-NDLNYNY-VPP-AESA--------KSGTCLLL---------------------------KEFYDDFSTGNIPIPWQDEMIETGVFGELNQWGPKGSTP-NDLNPNY-VP---ETT--------KSGTCALL---------------------------NEFYNEFSTGNVPIPWQEEMIETGVFGELNIWAPGGKLP-DDLDPNK-IP----VA--------KGGACTLL---------------------------TEFFDEFSSGNIPIPWQEEMIETGVFGELNVWAEKGKLP-PDLDPNF-V-----EA--------KGGACVLL---------------------------DEFYTEFATGNVPIPWQEEMIETGVFGELNIWGQNGKLP-NDLDPNF-V-----EA--------KGGGCVLL---------------------------QQFYKEFASGKMAIPWQEEIIETGVFDELNVWGDGGLTP-SD-------GEDPARS--------KSGTCLLL---------------------------KDFYNEFASGNITIPWQEEMIETGVFDEMNIWEEDGKVA-DE-------EEDSASR--------KSGTCVLL---------------------------KDFYNKFASGNMAIPWQEEMIETGVFDEMNIWGEDGKMP-IE-------VEDSASS--------KSGTCTLL---------------------------TEFFQEFATGNCPIPWQEEMIETGIFGELNVWRSDGQMP-DDMKGIS-GGS-SSSS--------KSGMCLVS---------------------------TEFFQEFASGTCPIPWQEEMIETGVFGDLNVWRPDGQMP-DDMKGVSGQEA-APSS--------KSGMCVLS---------------------------TEFFQEFATGNCPIPWQEEMIEMGIFGDLNVWRADGQMP-DDMKGIAVEES-PGAS--------KSGMCLIS---------------------------TEFFQEFATGNCPIPWQEEMIEMGIFGDLNVWRADGQMP-DDMKGTAVEEP-SSSS--------KSGMCLIS---------------------------NKLFDEFASGNISIPWQEEMVETGIFDEIN-----SELNENNVRRES-IVN-DNSA--------KSGVCRIA---------------------------KQLFDEFASGNIPLPWQEEMIETGIYDELN-----AEL--NDVRRYS-TAN-DNSA--------KSGVCQIA---------------------------KEFFDEFASGNISIPWQEEMIETGVFGELNVWGEGGKLP-NDLRRES-ILE-PPP---------KSGVCAVS---------------------------KEFFDEFASGNISIPWQEEMLETGVFGELNIWGAGGTLP-NDLRRES-IFE-PPP---------KSGVCSVS---------------------------KCFFDEFASGNISIPWQEEMIETGIYGELNVWGPGGAVP-NDLRRES-ILE-QPP---------KSSTCSVS---------------------------TVFFDEFASGNIAIPWQEEMIETGIYGELTVWGPGGALP-NDLRRES-ILE-QPP---------KSSTCCAS---------------------------KNFFDEFSSGNIPIPWQEEMIEMGIYSELNLWGPDGTVP-NDLRRES-ILE-QP----------KSSTCHIC---------------------------KEFFDEFATGNIPIPWQEEMIETGIFDELNVWGANGELP-SDLRRDP-FVE-PPSS--------KSGVCSIS---------------------------KEFFDEFATGNIPIPWQEEMIETGVFDELNVWGVNGGLP-NDLRRES-ILE-MPPS--------KSGVCSIS---------------------------KEFFDEFATGNISIPWQEEMIETGVFVELNVWGPNGGLP-NDLRRES-ILE-MPPS--------RSGVCTVA---------------------------KEFFDEFATGNISIPWQEEMIETGVFVELNVWGPNGGLP-NDLRRES-ILE-LPPS--------KSGVCIVA---------------------------KEFFDEFSTGNITIPWQEEMIETGLFGELNVWGPAGGLP-NDLRRDS-ILE-PPPS--------KSGVCCIA---------------------------NDFYAKFSTGSVSIPWQNEMCETSVYKDLNVFNADGSPT-PDLTPYDQQS-NPPKKGFFSRLFGRQVIILVLVVLYQE----------------VLKLGH ESFYSKFNTGSVSIPWQQEMIETECYQELNVFLEDGEPT-PDLI-MDQPPPLQDVVSSAEHSNDRCAVALVTTTTTTMLRGFSRRETRDWHEHMNNTDSH ESFYSKFNTGSVSIPWQQEMIETECYQELNVLSEDGEPT-PDLI-MDQPPP-PPRRGFFSRTFKRQVCCGSGNPDND-----------------NDAARV QDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVP-PDLDWKGQPP-APPKKGLLQRLFSRQRIAVETAATARKSS----PPASSPQPEAPTSSWR DDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLP-PDLN-RNHPP-EPPKKGLLQRLFKRQHQNNSKSSPSSKTS--FNHHINSNHVSSNSTGSS EDFYARFATGCVSIPWQNEMIESGCFKDINKSESEEALP-LDLDKNIHTPVSRPNRGFFYRLFRRGGCLTMVPSEKE-----------------VEPKQC ..630.......640.......650.......660.......670.......680.......690.......700.......710.......720.....

553 552 553 546 551 551 551 545 546 551 552 550 551 549 557 548 565 555 552 554 554 554 553 556 560 516 559 556 549 364 240 569 571 429 593 580 592 563 561 561 563 561 560 559 559 559 558 558 563 561 565 565 559 558 563 563 563 563 561 565 565 564 564 564 599 610 525 589 590 578

Arr-S_Human_NP-000532 Arr-S_Cattle_XP-010802144 Arr-S_Tasmanian_devil_XP-003770202 Arr-S_Koala_XP-020827934 Arr-S_Chicken_XP-015132693 Arr-S_Ground-tit_XP-005529624 Arr-S_Golden_eagle_XP-011575693 Arr-S-Partial_Turtle_XP-007052695 Arr-S_Anole_XP-016847626 Arr-S_Gecko_XP-015282299 Arr-S_Xenopus_NP-989073 Arr-S_Nanorana_XP-018427475 Arr-S_Florida_gar_22184-1-1 Arr-S_Bowfin_24232-1-2 Arr-Sa_Zebrafish_NP-956853 Arr-Sa_Medaka_NP-001116373 Arr-Sb_Medaka_NP-001098559 Arr-S1_Reef_shark_55938-25-1 Arr-S1_Bamboo_shark_45077-7-1 Arr-S1_Whale_shark_XP-020370169-XP-020368728 Arr-S1_Bluespot_ray_30422-1-1 Arr-S1_Western_ray_26221-2-1 Arr-S1_Elephant_shark_XP-007902404 Arr-S2_Reef_shark_55253-10-3 Arr-S2_Bamboo_shark_28962-1-1 Arr-S2_Whale_shark_XP-020368730 Arr-S2_Bluespot_ray_31414-1-1 Arr-S2_Western_ray_22644-1-1 Arr-S2_Elephant_shark_XP-007887798 Arr-VZ_E.cirrhatus_129805-1-1 Arr-VY_G.australis_54008-1-2 Arr-VX_M.mordax_42250-9-1 Arr-VX_G.australis_59149-4-1 Arr-VX_L.camtschaticum_BAJ83617 Arr-C_Human_NP-004303 Arr-C_Cattle_NP-776419 Arr-C_Opossum_XP-007507671 Arr-C_Koala_XP-020832166 Arr-C_Chicken_NP-001091002 Arr-C_Ground-tit_XP-005526200 Arr-C_Golden_eagle_XP-011578691 Arr-C_Turtle_XP-007055341 Arr-C_Anole_XP-008122024 Arr-C_Gecko_XP-015269615 Arr-C_Xenopus_XP-012823333 Arr-C_Nanorana_XP-018410246 Arr-C_Florida_gar_15939-1-1 Arr-C_Bowfin_14016-1-1 Arr-Ca_Zebrafish_NP-001002405 Arr-Cb_Zebrafish_NP-957086 Arr-C_Medaka_XP-011478269 Arr-C-Partial_Reef_shark_104042-1-1w50893-1-1 Arr-C_Bamboo_shark_45371-8-2 Arr-C_Whale_shark_XP-020383069 Arr-C_Bluespot_ray_36257-4-1 Arr-C_Western_ray_37242-1-1 Arr-C_Elephant_shark_XP-007892403 Arr-B1_Human_NP-004032 Arr-B1_Cattle_NP-776668 Arr-B1_Tasmanian_devil_XP-003764705 Arr-B1_Koala_XP-020845203 Arr-B1_Golden_eagle_XP-011583127 Arr-B1_Ground-tit_XP-005524198 Arr-B1_Turtle_XP-006127564 Arr-B1_Gecko_XP-015265943 Arr-B1_Python_XP-007440235 Arr-B1_Xenopus_XP-004912283 Arr-B1_Florida_gar_28267-2-1w1-11 Arr-B1_Bowfin_26818-1-6 Arr-B1_Zebrafish_NP-001153294 Arr-B1_Medaka_XP-011471558 Arr-B1-Partial_Reef_shark_38868-1-2 Arr-B1-Partial_Bamboo_shark_c25388-1-1w2-1 Arr-B1_Whale_shark_XP-020371759 Arr-B1-Non-contig_Western_ray_78258-1-1w66643-1-1w83393-1-1 Arr-BY_M.mordax_41599-5-1 Arr-BY-Non-contig_G.australis_41931-1-1w37543-2-1w1-1w53942-1-1 Arr-BX-Partial_M.mordax_41599-4-1 Arr-BX-Partial_G.australis_54326-4-1w1-1w2-3 Arr-BX-Partial-Trimmed_P.marinus_ENSPMAP00000004285 Arr-BX_L.camtschaticum_BAJ83616 Arr-B2_Human_NP-004304 Arr-B2_Cattle_NP-001192206 Arr-B2_Tasmanian_devil_XP-003770754 Arr-B2_Koala_XP-020822087 Arr-B2_Turtle_XP-006116398 Arr-B2_Python_XP-007444595 Arr-B2_Gecko_XP-015283361 Arr-B2_Xenopus_NP-001093721 Arr-B2_Nanorana_XP-018417500 Arr-B2-Partial_Spotted_gar_XP-015195812 Arr-B2-Non-contig_Bowfin_13774-1-1w18902-1-1 Arr-B2a_Zebrafish_NP-999846 Arr-B2b_Zebrafish_NP-957418 Arr-B2_Medaka_XP-004076705 Arr-B2_Reef_shark_51008-1-2 Arr-B2_Bamboo_shark_31008-1-1 Arr-B2-Partial_Bluespot_ray_34441-5-1 Arr-B2-Partial_Western_ray_34309-2-1 Arr_C.intestinalis_NP-001041447 Arr_C.savignyi_ENSCSAVP00000014619 Arr_H.roretzi_BAC76419 Arr_B.floridae_XP-002593261 Arr_B.belcheri_XP-019616407 Arr_S.kowalevskii_NP-001171767 krz_D.melanogaster_NP-524988

M--------------AASGKTSKSEPNH--------------------------------------------------------------VIFKK---ISRDKS--------------------------------VTIYLGNRDYIDHVSQVQPVDGVV M------------------KANKPAPNH--------------------------------------------------------------VIFKK---ISRDKS--------------------------------VTIYLGKRDYIDHVERVEPVDGVV M-----------------ATGVRNAPNN--------------------------------------------------------------VIFKK---VSRDKS--------------------------------VTIYLGKRDYIDHVDKVEPVDGVV M-----------------ATGVKNAPNH--------------------------------------------------------------VIFKK---VSRDKS--------------------------------VTIYLGKRDYIDHVDQVEPVDGVV M----LKMGLLCSLWNLQLRTTSSHP-------------------------------------------------------------------------------------------PGTQPPTLCSSTVVIFSLQLTIYLGKRDFIDNIGSVEPVDGVV M--SCPVSQK----TGIGGKNADSPPKQ--------------------------------------------------------------VIFRK---STRDKAVSTIYSSLMCVSPFPQQPQTFSSSTVVILSPQLTIYLGKRDFIDNMGNVEPVDGVV M--SCPESQK----TGTSGKNASSLHKQ--------------------------------------------------------------VVFKK---STRDKA--------------------------------LTIYLGKRDFIDNIGNVEPVDGVV M--SCAESHKVATLSSISLKNASPPQSH--------------------------------------------------------------VVYKK---MSRDKA--------------------------------VTIYLGKRDFIDHIDGVEPVDGVL M----------VSVSDISMKNVEPARSH--------------------------------------------------------------VIYKK---VSHDKA--------------------------------VTLYLGKRDFIDHVEDVEPVDGVV M----------------------SPQTH--------------------------------------------------------------VIYKK---ISRDKG--------------------------------VTIYLGKRDFIDHVDDVESVDGVV M-------------------SGDKKSRH--------------------------------------------------------------VIYKK---TSRDKA--------------------------------VSVYLGKRDYVDHVESVDPVDGVV M-------------------SGDKKSRS--------------------------------------------------------------VIYKK---TSRDKA--------------------------------VSVYLGKRDYVDHVESVDPVDGIV M----------------------SSSRH--------------------------------------------------------------IIFKK---LSRDKS--------------------------------VGVYMGKRDFVDHVDFVDPVDGVV M----------------------SSSRH--------------------------------------------------------------VIFKK---LSRDKS--------------------------------VGVYMGKRDFVDHCEYVDPVDGVV M-----------------------SPKN--------------------------------------------------------------VVFKK---ISKDKS--------------------------------VGVYMGKRDFVDRVDSVDPVDGVI M-----------------------SPKN--------------------------------------------------------------VIFKK---TCKDKS--------------------------------VGVFMGKRDFVDRVDSVDPVDGVI M-----------------------TPKQ--------------------------------------------------------------VIFKK---VSRDKS--------------------------------VAVYLAKRDFVDHCDFVDPVDGVV M--------------------SA-NKKN--------------------------------------------------------------IVYKK---LSRDKA--------------------------------IATYLSKRDFVDHGSHTDLVDGVL M--------------------SKDSKKN--------------------------------------------------------------VVYKK---VSRDKS--------------------------------IAAYLSKRDFVDHGSHVDPVDGVL M--------------------SKNSKKN--------------------------------------------------------------IVYKK---VSRDKS--------------------------------IAAYLSKRDFVDHGSHIDPVDGVL MSAD-----------------GKTSKKN--------------------------------------------------------------IVYKK---VTRDKS--------------------------------IAAYLSKRDFVDHGTHIDPVDGVL MSAD-----------------SKNSKKH--------------------------------------------------------------VVYKK---VSRDKS--------------------------------IAAYLSRRDFVDHGSHIDPVDGVL ------------------------------------------------------------------------------------------------------------------------------------------MYLGKRDFVDHLDHIEPVDGVI MSLPQTEKTPTPSVVIAKMNPPSAAPKN--------------------------------------------------------------VIFKK---TTPDKS--------------------------------IAVYTGKRDFIDHVTHTDLVDGVL MSAPQIQKTPAPSVVVAKMNPPPPAPKN--------------------------------------------------------------IVFKK---TTCDKS--------------------------------IAVYLGKRDFVDHVTHTDPVDGVL MNVPQIQKTPAPSIVVVKMNPSTPGPKN--------------------------------------------------------------IVFKK---TTSDKS--------------------------------IAVYLGKRDFVDHVTHTDPVEGVL MSIPQLQKVPAPSVVVTKMMSPPPARKN--------------------------------------------------------------LVFKK---TTTDKS--------------------------------IAVYLGKRDFVDHVSHTDPVDGVL MSNPQLQLNPAPSVVVAKMMPPLPGPKN--------------------------------------------------------------IVFKK---TTIDKS--------------------------------IAVYLGKRDFVDHVNHTDPVDGVL MSNSTVMKNPVPSLVIAKINPPIPAPKN--------------------------------------------------------------VVYKK---TSRDKS--------------------------------IAIYLGKRDFVDHVTHADPVDGVL M----------------------S--SV--------------------------------------------------------------SVFKK---RTEDGS--------------------------------LIVHLGKRDFVDFVDSVEPVDGVV M----------------------AAKAA--------------------------------------------------------------HVFKK---TSSNGK--------------------------------VTVYMAKRDFIDHLDYVDPVDGVV M----------------------AGGGN--------------------------------------------------------------RVFKK---PSPNGK--------------------------------ITLYLGKRDYVDHVDHVDPVEGII M----------------------GESGN--------------------------------------------------------------RVFKK---TSPNGK--------------------------------MTLYLGKRDYVDHVDHVDPIDGVV M----------------------GDSGN--------------------------------------------------------------RVFKK---TSPNGK--------------------------------ITLYLGKRDYVDHVDHVDPIDGIV M--------------------------S--------------------------------------------------------------KVFKK---TSSNGK--------------------------------LSIYLGKRDFVDHVDTVEPIDGVV M----------------------AN-MS--------------------------------------------------------------RVFKK---TCSNGK--------------------------------LSIYLGKRDFVDHVDMVEPIDGVV M----------------------AT-SS--------------------------------------------------------------KVFKK---TCPNGK--------------------------------LSIYLGKRDFVDHVDSVEPIDGVV M----------------------ST-SS--------------------------------------------------------------KVFKK---TCPNGK--------------------------------LSIYLGKRDFVDHIDSVEPIDGVV M----------------------AE-GS--------------------------------------------------------------KVFKK---TSPNGK--------------------------------LSIYLGKRDFVDHVESVDAVDGVC M----------------------AE-GS--------------------------------------------------------------KVFKK---TSPNSK--------------------------------LSLYLGKRDYVDNVDSVESVDGVC M----------------------AE-GA--------------------------------------------------------------KVFKK---TSPNSK--------------------------------LSIYLGKRDFVDHVESVDSVDGIC M----------------------SD-GS--------------------------------------------------------------KVFKK---TSPNGK--------------------------------LSIYLGKRDFVDHVGSVEPVDGVV M----------------------AD-GS--------------------------------------------------------------KVFKK---TSPNGK--------------------------------LSIYLGKRDFVDHVDCVDPVDGVV M----------------------SS-GL--------------------------------------------------------------KVFKK---TSPNGK--------------------------------LTIYLGRRDFVDHVESVEPVDGVV M----------------------AE-GS--------------------------------------------------------------KVFKK---SSADGK--------------------------------LSIYLAKRDYVDHVDHVEPVDGMI M----------------------AD-GS--------------------------------------------------------------KVFKK---TSPDGK--------------------------------LSIYLAKRDFVDHVESVEPVDGMI M----------------------PG-----------------------------------------------------------------KVFKK---TSPNGK--------------------------------LSLYLGKRDFVDHVDSVDPVDGVV M----------------------SA-----------------------------------------------------------------KVFKK---TSPNGK--------------------------------LSLYLGKRDYVDHIDNVDSVDGVV M----------------------AD-----------------------------------------------------------------KVYKK---TSGNGQ--------------------------------LTLYLGKRDYVDHVDVVDAVEGAV M----------------------T------------------------------------------------------------------KVYKK---TSGNGS--------------------------------LCLYLGRRDFVDHVESVDSVDGVL M----------------------A------------------------------------------------------------------KVFKK---TSGNGT--------------------------------IALYLGKRDFVDCVDSVEVVDGVV M----------------------AQKPG--------------------------------------------------------------QVFKK---TSPNEK--------------------------------ISLYLGKRDFVDHVDTVDPVDGML M----------------------TQKSG--------------------------------------------------------------QVFKK---TSPNAK--------------------------------LSLYLGKRDFVDHVDTVDPVDGML M----------------------TQKPG--------------------------------------------------------------QVFKK---TSPNAK--------------------------------LSLYLGKRDFVDHVDTVDPVEGML M----------------------SQKAG--------------------------------------------------------------KVFKK---TSPNQK--------------------------------LSLYLGKRDFVDHIDNVDPVDGVL M----------------------SKNSG--------------------------------------------------------------QVFKK---TSPNQK--------------------------------LSLYLGKRDFVDHIDKVDPIDGVL M----------------------AGNIG--------------------------------------------------------------KVFKK---MSKNAK--------------------------------LTLYLGKRDFVDHIDSVDPVDGAV MG----DKGT--------R------------------------------------------------------------------------VFKK---ASPNGK--------------------------------LTVYLGKRDFVDHIDLVDPVDGVV MG----DKGT--------R------------------------------------------------------------------------VFKK---ASPNGK--------------------------------LTVYLGKRDFVDHIDLVEPVDGVV M----------------------CVVFGVRWACSFPDGRR----------------C---------------------------------RVFKK---ASPNGK--------------------------------LTVYLGKRDFVDHIDIVDPVDGVV MSHLRKKMDW---------------------------------------------------------------------------------SWKRELHAQPSGK-------------------------LQSSGVLLTVYLGKRDFVDHIDIVDPVDGVV MG-----SGTIACPVLPWRDGNASRSTHG---CPLPQPLTPPPSLC--------SFC---------------------------------RVFKK---ASPNGK--------------------------------LTVYLGKRDFVDHIDVVDPVDGVV MG----DKGT--------R------------------------------------------------------------------------VFKK---ASPNGK--------------------------------LTVYLGKRDFVDHIDVVDPVDGVV MG----DKGT--------RAISAAVSSSV--------DPTASPTLA--------K-S---------------------------------VVFKK---ASPNGK--------------------------------LTVYLGKRDFVDHIDVVDPVDGVV MDGQLAEDGQ--------K------------------------------------------------------------------------VFKK---ASPNGK--------------------------------LTVYLGKRDFVDHIDVVDSVDGVV -------------------------------------------------------------------------------------------VFKK---ASPNGK--------------------------------LTVYLGKRDFVDHIDLVDPVDGVV MDYMWIDSPYLRIRTVLEESVPACVVAPLGIRCGELEAHKAAPMEN--------V-F---------------------------------LVFKK---ASPNGK--------------------------------LTVYLGKRDFVDHIDVVDPVDGVV MG--------------DKG-------------------------------------T---------------------------------RVFKK---ASPNGK--------------------------------LTVYLGKRDFVDHVDVVEPVDGVV MG--------------DKG-------------------------------------T---------------------------------RVFKK---ASPNGK--------------------------------LTVYLGKRDFVDHVDLVEPVDGVV MG--------------DKG-------------------------------------T---------------------------------RVFKK---ASPNGK--------------------------------LTVYLGKRDFVDHVDLVEPVDGVV MG--------------DKG-------------------------------------T---------------------------------RVFKK---ASPNGK--------------------------------LTVYLGKRDFVDHVDFVDPVDGVI ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MSEALFAAERSTGVSMDLE-------------------------------------L---------------------------------RVFKK---SSPNGK--------------------------------LTVYLGKRDFVDHVDFVEPVDGVV ------------------------------------------------------------------------------------------------------------------------------------------------DFVDHVDFVEPVDGVV MVD-------------KSG-------------------------------------T---------------------------------RVFKK---SSPNGK--------------------------------VTVYLGKRDFVDHLEHVDPVDGVV MGD-------------KSG-------------------------------------T---------------------------------RVFKK---SSPNGK--------------------------------VTVYLGKRDFVDHLEHVDPVDGVV ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGE-------------KPG-------------------------------------T---------------------------------RVFKK---SSPNGK--------------------------------VTVYLGKRDFVDHLEHVDPVDGIV M------------------------------------------GEK--------PGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDKVDPVDGVV M------------------------------------------GEK--------PGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDKVDPVDGVV M------------------------------------------GEK--------AGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDRVDPVDGVV M-----------------------------------------LGHREEQQVACYPQG---------------------------------QVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDRVDPVDGVV M-----------------------------------------------------PLP---------------------------PPLFLPRVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDRVDPVDGVV MVAGGSWDFMGRKKAGGAELVRRPTPASALTAQAEPRGGKGLPGRR--------PGAQEQARGAVRLDSPGAGKGALCNYILFFVPIFCNRVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDRVDPVDGVV M-------------------------------------------------------QKLHSRIIWLWSDVPFGTVRKCSY-------FIRWVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDRVDPVDGVV M------------------------------------------GER--------AGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDRVDPVDGVV M------------------------------------------GER--------AGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDRVEPVDGVV MG-----------------------------------------DK---------AGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDHVDPVDGVL ------------------------------------------------------AGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDHVDPVDGVL MG-----------------------------------------DK---------AGT---------------------------------RVFKK---SSPNCK--------------------------------VTVYLGKRDFVDHLDHVDPVDGVI MG-----------------------------------------DK---------AGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDHVDPVDGVL MG-----------------------------------------DK---------AGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDHVDPVDGVI M------------------------------------------GDK--------SGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDHVDPVDGVV M------------------------------------------GDK--------SGT---------------------------------RVFKK---SSPNCK--------------------------------LTVYLGKRDFVDHLDHVDPVDGVV ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MA----------------------------------------DKG---------AGT---------------------------------RVFKK---SSPNGK--------------------------------VTAYLGKRDYVDHIDHTDPIDGVV MA----------------------------------------DKG---------AGT---------------------------------RVFKK---SSPNGK--------------------------------VTAYLGKRDYVDHIDHTDPIDGVV M------------------------------------------SS---------AGT---------------------------------RVFKK---SCPNGK--------------------------------ITTYLGKRDYVDHITTTDPIDGVV MLAADGDGG-------------YNGPAANMDTSEETGNDGKDQKK---------QGT---------------------------------RVFKK---SSPNGK--------------------------------ITVYLGKRDFVDHLTHVDPVDGVI MLAADGDGG-------------YNGPAANMDTSEETGNDGKDQKK---------QGT---------------------------------RVFKK---SSPNGK--------------------------------ITVYLGKRDFVDHLTHVDPVDGVI MASTTEKQS-------------DNGPKM-----EGSQDQLKKAGT---------RGS---------------------------------RVFKK---SSPNGK--------------------------------ITTYLGKRDFVDHLTHVDPVDGVI MNGGGGGSGGANVSNGSSTNVTAAGSTGGGSAGDETGGDA-SSRR---------QAT---------------------------------RVFKK---SSSNGK--------------------------------ITVYLGKRDFVDHVTHVDPIDGVV 1.......10........20........30........40........50........60........70........80........90.......100.......110.......120.......130.......140.......150.......160

49 45 46 46 65 89 57 61 53 41 44 44 41 41 40 40 40 42 43 43 46 46 22 63 63 63 63 63 63 39 41 41 41 41 37 40 40 40 40 40 40 40 40 40 40 40 38 38 38 37 37 41 41 41 41 41 41 41 41 54 54 76 41 63 45 34 83 41 41 41 41 55 16 42 42

42 42 42 42 51 45 117 63 42 42 42 38 42 42 42 42 42 43 43 41 70 70 65 82

Arr-S_Human_NP-000532 Arr-S_Cattle_XP-010802144 Arr-S_Tasmanian_devil_XP-003770202 Arr-S_Koala_XP-020827934 Arr-S_Chicken_XP-015132693 Arr-S_Ground-tit_XP-005529624 Arr-S_Golden_eagle_XP-011575693 Arr-S-Partial_Turtle_XP-007052695 Arr-S_Anole_XP-016847626 Arr-S_Gecko_XP-015282299 Arr-S_Xenopus_NP-989073 Arr-S_Nanorana_XP-018427475 Arr-S_Florida_gar_22184-1-1 Arr-S_Bowfin_24232-1-2 Arr-Sa_Zebrafish_NP-956853 Arr-Sa_Medaka_NP-001116373 Arr-Sb_Medaka_NP-001098559 Arr-S1_Reef_shark_55938-25-1 Arr-S1_Bamboo_shark_45077-7-1 Arr-S1_Whale_shark_XP-020370169-XP-020368728 Arr-S1_Bluespot_ray_30422-1-1 Arr-S1_Western_ray_26221-2-1 Arr-S1_Elephant_shark_XP-007902404 Arr-S2_Reef_shark_55253-10-3 Arr-S2_Bamboo_shark_28962-1-1 Arr-S2_Whale_shark_XP-020368730 Arr-S2_Bluespot_ray_31414-1-1 Arr-S2_Western_ray_22644-1-1 Arr-S2_Elephant_shark_XP-007887798 Arr-VZ_E.cirrhatus_129805-1-1 Arr-VY_G.australis_54008-1-2 Arr-VX_M.mordax_42250-9-1 Arr-VX_G.australis_59149-4-1 Arr-VX_L.camtschaticum_BAJ83617 Arr-C_Human_NP-004303 Arr-C_Cattle_NP-776419 Arr-C_Opossum_XP-007507671 Arr-C_Koala_XP-020832166 Arr-C_Chicken_NP-001091002 Arr-C_Ground-tit_XP-005526200 Arr-C_Golden_eagle_XP-011578691 Arr-C_Turtle_XP-007055341 Arr-C_Anole_XP-008122024 Arr-C_Gecko_XP-015269615 Arr-C_Xenopus_XP-012823333 Arr-C_Nanorana_XP-018410246 Arr-C_Florida_gar_15939-1-1 Arr-C_Bowfin_14016-1-1 Arr-Ca_Zebrafish_NP-001002405 Arr-Cb_Zebrafish_NP-957086 Arr-C_Medaka_XP-011478269 Arr-C-Partial_Reef_shark_104042-1-1w50893-1-1 Arr-C_Bamboo_shark_45371-8-2 Arr-C_Whale_shark_XP-020383069 Arr-C_Bluespot_ray_36257-4-1 Arr-C_Western_ray_37242-1-1 Arr-C_Elephant_shark_XP-007892403 Arr-B1_Human_NP-004032 Arr-B1_Cattle_NP-776668 Arr-B1_Tasmanian_devil_XP-003764705 Arr-B1_Koala_XP-020845203 Arr-B1_Golden_eagle_XP-011583127 Arr-B1_Ground-tit_XP-005524198 Arr-B1_Turtle_XP-006127564 Arr-B1_Gecko_XP-015265943 Arr-B1_Python_XP-007440235 Arr-B1_Xenopus_XP-004912283 Arr-B1_Florida_gar_28267-2-1w1-11 Arr-B1_Bowfin_26818-1-6 Arr-B1_Zebrafish_NP-001153294 Arr-B1_Medaka_XP-011471558 Arr-B1-Partial_Reef_shark_38868-1-2 Arr-B1-Partial_Bamboo_shark_c25388-1-1w2-1 Arr-B1_Whale_shark_XP-020371759 Arr-B1-Non-contig_Western_ray_78258-1-1w66643-1-1w83393-1-1 Arr-BY_M.mordax_41599-5-1 Arr-BY-Non-contig_G.australis_41931-1-1w37543-2-1w1-1w53942-1-1 Arr-BX-Partial_M.mordax_41599-4-1 Arr-BX-Partial_G.australis_54326-4-1w1-1w2-3 Arr-BX-Partial-Trimmed_P.marinus_ENSPMAP00000004285 Arr-BX_L.camtschaticum_BAJ83616 Arr-B2_Human_NP-004304 Arr-B2_Cattle_NP-001192206 Arr-B2_Tasmanian_devil_XP-003770754 Arr-B2_Koala_XP-020822087 Arr-B2_Turtle_XP-006116398 Arr-B2_Python_XP-007444595 Arr-B2_Gecko_XP-015283361 Arr-B2_Xenopus_NP-001093721 Arr-B2_Nanorana_XP-018417500 Arr-B2-Partial_Spotted_gar_XP-015195812 Arr-B2-Non-contig_Bowfin_13774-1-1w18902-1-1 Arr-B2a_Zebrafish_NP-999846 Arr-B2b_Zebrafish_NP-957418 Arr-B2_Medaka_XP-004076705 Arr-B2_Reef_shark_51008-1-2 Arr-B2_Bamboo_shark_31008-1-1 Arr-B2-Partial_Bluespot_ray_34441-5-1 Arr-B2-Partial_Western_ray_34309-2-1 Arr_C.intestinalis_NP-001041447 Arr_C.savignyi_ENSCSAVP00000014619 Arr_H.roretzi_BAC76419 Arr_B.floridae_XP-002593261 Arr_B.belcheri_XP-019616407 Arr_S.kowalevskii_NP-001171767 krz_D.melanogaster_NP-524988

** .:. LVDPDLVKGKKVYVTLTCAFRYGQ-EDIDVIGLTFRRDLYFSRVQVYPPV-----------GAAS--TPTKLQESLLKKLGSNTYPFLLTFPDYLPCSVMLQPAPQDSGK--SCGVDFEVKAFAT---DSTDAEE--DKIPKKSSVRLLIRKVQHAPLEM LVDPELVKGKRVYVSLTCAFRYGQ-EDIDVMGLSFRRDLYFSQVQVFPPV-----------GASG--ATTRLQESLIKKLGANTYPFLLTFPDYLPCSVMLQPAPQDVGK--SCGVDFEIKAFAT---HSTDVEE--DKIPKKSSVRLLIRKVQHAPRDM LVDPELVKGKKVFVTLTCAFRYGQ-EDIDVIGLTFRRDLYFSKVQIYPPV-----------STAN--KPTKLQESLLKKLGSNAYPFLLTFPDYLPCSVALQPAPQDVGK--CCGVDFEVKAYAT---ENTEIEE--EKVPKKSSVRLLIRKVQHAPPEL LVDPELVKGKKVFVSLTCAFRYGQ-EDIDVIGLTFRRDLYFSRVQAYPPI-----------NTGH--TPTRLQESLLKKLGINAYPFLLTFPDYLPCSVALQPAPQDVGK--CCGVDFEVKAYAT---DITDTEE--ERVPKKSSVRLLIRKVQHAPPEM LVDPAMIKGKKVYVTLTCAFRYGQ-EDIDVIGLTFRRDLFFSRVQVYPPE-----------NRPE--SLSHLQESLLKKLGKNAYPFYFTFPDYLPCSVCLQPSPRDVDK--SCGVDFEVKAFST---ENL---E--ERIHKRNSARLLIRKVQFAPEEP LVDPAIVQGKKVFVSLTCVFRYGQ-EDIDVIGLAFRRDLFFSRVQVYPPA-----------DKPE--SLTLLQESLLKKLGKNAYPFFFTFPDYLPCSVCLQPAPRDVDK--TCGVDFEVKAFST---ENV---E--ERIHKRNSARLLIRKVQYAPEEP LVDPAIIKGKKVYVSLTCAFRYGQ-EDIDVIGLTFRRDLFFSRVQVYPPA-----------DKPE--SLSHLQESLLKKLGKNAYPFFFTFPDYLPCSVCLQPAPRDVDK--SCGVDFEVKAFST---DNV---E--ERIHKRNSARLLIRKVQYAPEKP LVDPEIIKGKKVYVTLTCAFRYGQ-EDIDVMGLTFRKDLFFSRIQLYPPV-----------EKWE--SLSQLQECLMKKLGNNAYPFVLTFPDYLPCSVSLQPAPNDVGK--CCGVDFEVKAFCT---ENV---E--ERIPKRNFVRLLIRKVQFAPEEP MVDPDIVKNKKVFVTLTCAFRYGD-EDIDIIGLTFRRDLYISRVQVYPSL-----------EKHM--PLSSLQECLINKLGKNAYPFMLTFPDYLPCSVCLQPGLRDHGK--CCGVDFEIKAFAA---DDP---E--ETVLKRNSVRLMIRKIQYAPEVP LVDPEIVKEKKVYVTLTCAFRYGQ-EDIDVMGLTFRRDLYFSRTQVYPTL-----------EKQR--PLSDLQTCLLNKLGENAYPFVLTFPDYLPCSVSLQPAPHDVGK--CCGVDFEVKAFST---DNV---E--GRILKRNSVRLLVRKVQHAPEAP LVDPDLLKGKKVYVTLTCAFRYGQ-EDIDVIGLTFRKDLYFARTQIYPPV-----------EDPK--ALTKVQERLMKKLGNNAYPFLLQFPDYLPCSVSLQPAPTDVGK--ACGVDFEIKAFAT---NNL---E--DRIHKKNSVRLMIRKIQYAPDQP IVDPDLLKGKKVYITLTCAFRYGN-EDIDVIGLTFCKDLYFARTQVYPPV-----------EDVK--CLTKVQERLMKKLGNNAYPFMVAFPDFLPCSVALQPSPSDVDK--ACGVDFEIKAFSA---SNL---E--ERVHKKNSVRLLIRKIQYAPDQP LVDPELLKGKKVYIMLSCTFRYGR-DDMDVMGIAFRRDIYLSTRQVYPPL-----------QDRK--THSKTQERLLRKLGDNSYPFFFEFPDNLPCSVGLQPAPTDTGK--FCAVDFEIKAFCA---DNQ---D--DKVHKRSSVRLAIRKVQYAPEQR LVDPELLKGKKVYIMLSCTFRYGR-DDMDVLGVAFRRDIYLSTRQVYPPL-----------QDRN--THTKMQERLLRKLGVNAYPFFFEFPDNLPCSVGLQPAPSDIGK--YCAVDFEVKAFCA---ENQ---D--DKIHRRSSVRLTIRKVQYAPEQS LIDPEQLRGKKAYVTLSCVFRYGRDDDAEVLGISFRKEIYISTRQVYPAL-----------QDKEQCILTKVQEKLLRKLGDNAYPFFFEFPDNLPCSVGLQPAPKDVGK--HCAVEFEVKAFCA---ESQ---D--AKVRKRSSVGLMIRKVQYAPEKL LIDPEVLAGRKVFVTLSCTFRYGR-DDMDVMGIAFRRELYLVTRQVYPPM-----------QDRDKTVHTRVQAKLLRKLGNNAYPFFFEFPDNLPCSVALQPAPNDVGK--QCAVEFEIKAFSA---ESQ---D--AKVRKRSTVKLMIRKVQYAPESQ VIDPTQLKGRKVYVMLSCTFRYGR-QDMDVMGVAFRRDLFLVTRQVYPEL-----------QDKEKLTHTKIQQKLLRKLGDNAFPFFFEFPDNLPCSVSLQPGPLDEGK--KCAVEFEVKAFCG---EAQ---D--EKIDKQSSVRLTIRKIQFSPENT LVDTGFLKEQKVYCRLNCIFHYGS-EDIEMIGQAFKRDLYVSTIQVYPPT-----------GEQAK-PLTKAQERLMKKIGDKALPFCFEFPNHLPCSVCLQPAANDPEK--FCSVDFEVLAFCV---NSL---E--DKIRKRSSVRLTIRKIQYAPDRS LVDTGYIKDQKVYCRLSCIFHYGP-EDIEMIGQAFKRDLYVSTIQVYPPT-----------DSQVK-PVTKMQERLMRKIGDKALPFCFEFPNYLPCSVCLQPGANDPEK--FCSVEFEVLAFCV---NNL---E--EKIHKRSSVHLAIRKIQYAPNQS LVDTGFIKDQKAYCRLSCIFHYGP-EDIEMIGQAFKRDLYVSTIQVYPPT-----------GGQVK-PLTKTQERLMRKIGDKALPFCFEFPNHLPCSVCLQPGANDPEK--FCSVAFEVLAFCV---SSL---E--EKIRKRSSARLAIRKIQYAPGRS LVDAGYLRDLKVYCRLKCTFHYGP-EDIEMIGQAFQRDLYVSTIQIFPQA-----------GEEPK-SLTKMQERLMKKLGDKALPFTFQFPNYLPCSVCLQPGANDPDK--FCSVDFEVTAFCV---SSL---E--EKIHKRSSVRLSIRKVQYAPDRA LVDTGYVKDQKVYCRLKCTFHYGP-EDIEMIGQAFTRDLYVSTIQVYPQT-----------GEESK-SVTKMQERLMRKIGDKALPFSFQFPNYLPCSVCLQPGVNDSDK--FCSVDFEVIAFCV---SNL---E--EKIHKRSSVRLVIRKVQYAPDRA VVDTGYVKDRKIYCRLSCIFHYGK-EDMDVIGHPFSRDLYVSTIQIYPPT-----------EETAH-PLTKLQERLLKKVGDKAYPFCFQLPDHLPCSVYLQPSPSEPDK--CCSVDFEILSFCV---QSL---E--DKIHKKSSSRLMIRKVQFAPDKS VLDTGYIKDRKVYCRLLCSFRYGR-EDVDVCGLKFRRDLYETTLQVYPPP-----------SGEKPKPQTKLQERLVKKFGDKAYPFHFQFPDYLPCSITLQLGPNEIDK--CCGVDFQISAFCA---KTLNI-D--MKISKRSSVFMVIRKMQYAPDKP VLDTGYIKDRKVFCRLLCAFRYGR-EDIDVCGLKFRRDLYESTVQVYPPP-----------SGEKAKAQTKLQERLERKFGDKAYPFYFQFPDYLPCSVTLQLGANEIDK--CCGVDFQILAFCA---KTLSI-D--EKISKRSSVSMVIRKVQYAPDKP VLDTGYIKDKKVFCRLLCAFRYGR-EDIDVCGLKFRRDLHEATVQVYPPP-----------SGEKPKPQTKLQERLARKFGDKAYPFNFQFPDYLPCSVTLQLGPNEIDK--CCGVDFQILAFCA---KTLSI-D--EKISKRSSVSMVIRKVQYAPDKP VLDTGYIKDRKVFCRLLCAFRYGR-EDIDVCGLKFRRDLYMTTVQVYPPP-----------SGEKPKPQTKLQERLARKFGDRAYPFCFQFPDYLPCSITLQLGPNEIDK--CCGVDFQVIAFCA---KSCSY-D--EKISKKSSVAMVIRKVQYAPDKP VLDTGYIKDRKVFCRLLCAFRYGR-EDIDVCGLKFRRDLYMATIQVYPPP-----------SGEKPKPETKLQERLARKFGDKAYPFCFQFPDYLPCSITLQLGPNEVDK--CCGVDFQIIAFCA---KSSSY-D--EKISKRSSVSMVIRKVQYAPDKP VLDTGYIKSKKVYCRLVCAFRYGP-EDMEVCGLKFQRDLFVKVIQIYPPP-----------PEGKPKTQTKMQERLIKKFGDNAYPFNFQFPDYLPCSITLQVGLKNLDK--CCGVDFQILAFCA---KSLSI-D--EKISKRSSVNLVIRKVQFAPDKP LVDPDHVKDQRVYVSITGVFRYGR-QDMDVIGMPFRRDIYIAHVQLYPQM-----------SDH-PLQLTHLQQLLLNKLGPNAFPFTFTMPPNLPCSVMLQPGPNDKDK--MCCVDFQVTAFCA---KSL---Q--DDMRKKSSVCLLVRKVQFAPIAP YVDPAYIKGRKVFVTLTCAFRYGR-EDLDVMGIPFRRDIHVIRRQLYPPNRDPQTGGGGGPPQSAGGASSALQEKLLNKLGGTAYAFSFSMPQNLPCSVMMQPAPSDKGK--SCGVDFEVKAFCC---ESL---D--EDIKKKHSVSLLIRKVQYAPEKP TVDPEYLKDRKAYVQLSCAFRYGR-EDMDVLDVKFRKEIYAATTQVFPPL-----------PEDTRPSLTSLQERLVKKIGDNSFPFVFSLPENLPCSVSLQPGSDDTSK--SCGVDFVVKAFCA---ENL---E--EKIHRRNSVRLVIRKVQFAAERS VLDPEYLKERKAYIQMTCAFRYGR-EDLDVLGMTFRKDIHVMTTQLCPPL-----------PDE-KPTLTSLHERLVKKTGDNSYPFVFNLPQNLPCSVTLQPGPDDAGK--ACGVDFEVKAFCA---ENL---E--EKIHKRNSVRLLIRKVQFAPAGS ILDPEYLKERKAYVQMTCAFRYGR-DDLDVLDMTFRKEIYVSTMQVFPPL-----------PED-KKPLTQLQERLVKKTGDNSFPFVFELPQNLPCSITLQPGPDDAGKQ-ACGVDFEVKAFCA---ENL---E--EKIHKRNSVRLLIRKVQFAPERS LVDPEYLKCRKLFVMLTCAFRYGR-DDLEVIGLTFRKDLYVQTLQVVPAE-----------SSSPQGPLTVLQERLLHKLGDNAYPFTLQMVTNLPCSVTLQPGPEDAGK--PCGIDFEVKSFCA---ENP---E--ETVSKRDYVRLVVRKVQFAPPEA LVDPEYLKGRKMFVMLTCAFRYGH-DDLDVIGLTFRKDLYVQVQQVVPAE-----------SSSPRGSLTVLQERLLHKLGDNAYPFTLQMVVNLPCSVTLQPGPDDTGK--ACGVDFEVKSFCA---ENL---E--EKVSKRDSVRLVIRKIQFAPLEP LVDPEYMKDQKVFVVLTCAFRYGR-DDLDVIGLTFRKDLYVQALQVLPRE-----------SPEAPLALTALQECLLRKLGANAHPFTFQLAPNLPCSVTLQPGPEDAEK--ACGVDFEVKSFYA---ENL---E--EKIHKRNSVRLIIRKVQFAPSDL LIDPEHLKDRKVFVVLTCAFRYGR-DDLDVIGLTFRKDLYVQALQVLPKE-----------PTQDPLPLTNLQECLLHKLGDNAHPFTFQLPTNLPCSVTLQPGPEDAEK--ACGVDYEVKSFYA---ENL---E--EKIHKRNSVRLIIRKVQFAPLDL LIDPEYLKDRKVYVTLTCAFRYGR-DDLDVIGLTFRKDLYVLTTQIFPPV-----------PEQVPKTLTSLQEKLLKKLGENAYPFTFEIATNLPCSVTLQPGPDDVGK--ACGVDFEVKGFCA---ENL---E--EKIHKRNSVRLVIRKIQFAPMKT LIDPEYLKDRKVYVTLTCAFRYGR-DDLDVIGLTFRKDIYVLTTQLYPPV-----------PDQAPKTLTPLQEKLMKKLGENAYPFTFEIATNLPCSITLQPGPDDVGK--ACGVDFEVKGFCA---ENL---E--EKIHKRNSVRLIIRKVQFAPAQT LIDPEYLKDRKVYVTLTCAFRYGR-DDLDVIGLTFRKDLYVLTTQIFPPV-----------PDQTPKTLTPLQEKLMKKLGENAYPFTFEIATNLPCSVTLQPGPDDVGK--ACGVDFEVKGFCA---ENL---E--EKIHKRNSVRLIIRKIQFAPLMM LIDPEYLKDRKVYVILTCAFRYGR-DDLDVIGLTFRKDLYVLTTQIYPPV-----------PEQTPTSLTPLQEKLLKKLGEHAYPFTFEMATNLPCSVTLQPGPDDLGK--ACGVDFEVKGFCA---ENL---E--EKIHKRNSVRLVIRKVQFAPVNT LIDPEYLKDRKVYVTLTGAFRYGR-DDLDVIGLTFRKDLYVLVTQIFPPV-----------PDQTPKTLTPLQDKLLKKLGENAYAFTFEMATNLPCSVTLQPGPDDVGK--SCGVDFEVKGFCA---ENL---E--EKIHKRNSVRLVIRKIQFAPVST LIDPEYLKDRKVYVTLTCAFRYGR-DDLDVIGLTFRKDIYVLSTQIYPPV-----------PDQAPKSLTTLQEKLLRKLGEHAYPFTFEMATNLPCSVTLQPGPDDTGK--SCGVDFQVKGFCA---DNL---E--EKIHKRNSVRLVIRKVQFAPKNP LIDPEYQKDKKVFVTLTCTFRYGR-DDHELIGLSFKKDLYFLHCQVYPPL-----------PEDK-KALTPLQEKLAKKLGQNAFPFCFNMATDLPCSVTLQPGPEDTGK--KCGVDFEVKGFCA---ENV---E--EKVPRKNSVQLIIRKVQFAPEAT LIDPEYQKEKKVFVILTCAFRYGR-DDMELIGLCFRKDLYVQSCQVYPPL-----------PADK-KPLTPLQEKLKAKLGVNSFPFCFTMVKNLPCSVTLQPGPEDSGK--ACGVDFEVKGFWG---NDV---E--EKVSKKNAARLIIRKVQYAPEST LVDPEYLGDRKAYVTMACAFRYGR-DDLDVIGLSFRKDIYLATLQIYPPE-----------GGE-KPPNTPMQEQLLKKLGANAYPFHFEIPNNLPCSVTLQPGPDDVGK--SCGVDFEIKGFCA---ENL---E--EKIHKRNSVRLIIRKVQFAPDNT LVDPEYLGDRKAYVVMACAFRYGR-DDLDVIGLSFRKDIYLATIQVFPPE-----------AGE-KPPNTHMQNQLLKKLGPNAHAFHFKIPTNLPCSVTLQPGPDDVGK--ACGVDFEVKGFCA---ENL---E--EKIHKRNSVRLIIRKVQFAPVNT KIDPADLGDKKVWVQLACAFRYGR-EDLDVIGLSFRKDIWIQHIQLYPE------------AGH-KPTLTEMHNTLLKKAGEQGHPFTFNIPTNLPCSVTLQPGPDDKGK--ACGVDFEVKAYVAKSADDP---D--EKVDKKDTCRLVIRKIQFAPDNT KIDPSGLNGRKVWVQLACAFRYGR-EDLDVIGVSFRKDIWIKRIQMYPF------------EGT-KPPNTPMQEALLKKAGDQGHPFTFDIPVHLPCSVSLQPAPEDAGK--PCGVDYEVKAYIANEEDNI---D--EKVEKKDTCRLIIRKIQYAPAEL KVDPAGLNGKKVFVYLACAFRYGS-DDLDVIGLSFRRDIWIKRVQVYPV------------PAA-KAPSTPLQESLLKKVGDQGCPFTFQMPLNLPCSVSLQPGSGDSGK--ACGVDFEVKAYIADEADNP---D--EVIEKKNTCRLIIRKIQFAPANN LLDPEYLKDRKAYVTLACAFRYGH-EDLDVMGLSFRKDIYCETIQVYPPV-----------TDN-KKPLTPLQERLANKLKENCYPFIFQIPANLPCSVSLQPGAEDHRK--TCGVDFEVKAFCA---ENL---E--EKIHKRNSVKLIIRKVQYAPEKS LLDPDYLKDRRAYVMLTCAFRYGR-EDLDVMGLSFRKDIYCETIQVYPPV-----------SDN-KKPLTPLQEQLANKLKENCYPFLFQIPPNLPCSITLQPGPEDHGK--ACGVDFEVKGFCA---DNL---E--EKIHKRNSVKLIIRKVQYAPEKS LFDPDYLKDRKAYVTLTCAFRYGR-EDLDVMDLSFQKDIYSETIQVYPPI-----------AGN-KMPLTPLQERLASKLKENCYPFIFQIPPNLPCSITLQPGSEDHGK--ACGVDFEVKGFYA---DNL---E--EKIHKRNSVKLVIRKIQYAPEKS LIDPEYLKDRKVYVLLTCAFRYGR-EDLDVIGLSFRKDIYSEAIQVYPPL-----------SKN-KKQLTSLQEKLTNKLKENCYPFCFEIPTNLPCSVTLQPGPDDHGK--ACGVDFEVKAFCA---DSL---E--EKIHKRNSVRLIIRKVQYAPEQG LIDPEYLKDRKVYVVLTCAFRYGR-EDLDVMGLSFRKDIYLEAIQVYPPL-----------PEN-KKQPTPLQEKMINKLKENCYPFCFEVPTNLPCSVTLQPGPEDHGK--ACGVDFEVKAFCA---DNL---E--EKIHKRNSVRLIIRKVQYAPEQS LIDPEYLKDRKVYVTMTCAFRYGR-DDLDVMGLTFRKDIYFDAYQVYPPL-----------PEN-KRELTCLQTKLIHKLGENCYPFFFKIPTNLPCSVTLQPGQDDIGK--SCGVDFEVKGFCA---ENL---E--EKIHKRNSVCLIIRKVQFAPEES LVDPEYLKERRVYVTLTCAFRYGR-EDLDVLGLTFRKDLFVANVQSFPPA-----------PED-KKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPERP LVDPEYLKERRVYVTLTCAFRYGR-EDLDVLGLTFRKDLFVANVQSFPPA-----------PED-KKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPERP LVDPEYLKERRVYVTLTCAFRYGR-EDLDVLGLTFRKDLFVANIQSFPPA-----------PED--RPLTRLQERLIRKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ENL---E--EKTHKRNSVRLVIRKVQYAPERP LVDPEYLKERRVYVTLTCAFRYGR-EDLDVLGLTFRKDLFVANIQSFPPA-----------PED--RPLTRLQERLIRKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ENL---E--EKTHKRNSVRLVIRKVQYAPERP LVDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANAQAFPPV-----------PEE-KKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPERP LVDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANSQAFPPV-----------PED-KKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPERP LVDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANIQAFPPV-----------PEE-KKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPERP LVDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANIQAFPPV-----------AEE-KKPLTRLQERLIKKLGEQAYPFTFEIPPSLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ENP---E--EKIHKRNSVRLVIRKVQYAPERP LVDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVSNIQAFPPV-----------AEE-KKPLTRLQERLVKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ETL---E--EKIHKRNSVRLVIRKVQYAPERP LVDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANIQAFPPV-----------PEE-KKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDYEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPERP LIDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANIQAFPPV-----------PEE-KKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDFEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPEKP LIDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANIQAFPPV-----------PEE-KKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDFEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPEKP LIDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANIQAFPPV-----------PEE-KKSLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDFEVKAFCA---ENV---E--EKIHKRNSVRLVIRKVQYAPEKP LIDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFLANIQAFPPL-----------PEE-KKSLTRLQERLIKKLGEHAYPFIFE----------------------ACGVDFEVKAFCA---ENA---E--EKIHKRNSVRLVIRKVQYAPEKP -------------VTLTCAFRYGR-EDLDVLGLTFRKDLFVANVQAFPPV-----------AEE-KKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDFEVKTYCA---ENL---E--EKIHKRNSVRLVIRKVQYAPEKP -----------------------R-EDLDVLGLTFRKDLFVANVQAFPPI-----------VEE-RKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDFEVKTYCA---ENL---E--EKIHKRNSVRLVIRKVQYAPEKP LVDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANVQAFPPI-----------VEE-RKPLTRLQERLIKKLGEHAYPFTFEIPPNLPCSVTLQPGPEDTGK--ACGVDFEVKTYCA---ENL---E--EKIHKRNSVRLVIRKVQYAPEKP LVDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLFVANVQAFPPV-----------AEE-KKPLTRLQERLMKKLGEHGYPFVFEIPPNLPCSVTLQPGPEDTGK--ACGVDFEVKTFCS---DNL---X--XXXXXXXXXXXXXRKVQYAPEKP LVDPEYLKERKVFVTLTCAFRYGR-EDLDVLGLTFRKDLYLSTVQVYPPS-----------GDE-PRPSTRLQERLIRKIGDHAHPFTFQLPQNLPCSVTLQPGPEDTGK--ACGVDFEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPEKP LVDPEYLKERKVFVTL-XXXXXXX-XXXXXXXXXXRKDLYLSTVQVYPPS-----------ADE-PRPTTRLQERLIRKIGDHAHPFTFQLPQNLPCSVTLQPGPEDTGK--ACGVDFEVKAFCA---ENL---E--EKIHKRNSVRLVIRKVQYAPEKP ----------------------------------------------------------------------------------------------------------------------------------------------------VIRKVQFAPEKP -------------------------EDLDVLGLTFRKDLYLSTLQAVPPV-----------PEE-ARPLTRLQERLVKKLGKDAYPFTFEIPQNLPCSVTLQPGPEDTGK--ACGVDFEIKAFCA---ENL---E--EKIHKRNSVRLVIRKVQFAPEKP ---------------------------------------------------------------------------------------------------------------------------------------------RRNSVRLVIRKVQFAPEKP LVDSEYLKERKVYVTLTCAFRYGR-EDLDVLGLTFRKDLYLSTLQAVPPV-----------PEE-ARPLTRLQERLVKKLGKDAYPFTFEIPQNLPCSVTLQPGPEDTGK--ACGVDFEIKAFCA---ENL---E--EKIHKRNSVRLVIRKVQFAPEKP LVDPDYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLFIATYQAFPPV-----------PNP-PRPPTRLQDRLLRKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KSL---E--EKSHKRNSVRLVIRKVQFAPEKP LVDPDYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLFIANYQAFPPT-----------PNP-PRPPTRLQERLLRKLGQHAHPFFFTIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KSL---E--EKSHKRNSVRLVIRKVQFAPEKP LVDPDYLKDRKVYVTLTCAFRYGR-EDLDVLGLSFRKDLFAATYQAFPPI-----------PDP-PQTTTRLQERLLRKLGQHAHPFSFTIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KTL---E--EKIHKRNSVRLVIRKVQFAPETP LVDPDYLKDRKVYVTLTCAFRYGR-EDLDVLGLSFRKDLFAATYQAFPPI-----------PDP-PQPTTRLQERLLRKLGQHAHPFSFTIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KTL---E--EKIHKRNSVRLVIRKVQFAPETP LVDPEYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLFVATCQAVPTL-----------PQE-QCSPTRLQERLLPQLGAHAHPFSFTIPQNLPCSVTLQPGPEDTGK--ACGVDYEIRAFCA---KSL---E--EKIHKRNSVRLVIRKVQYAPEKP LVDPEYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLFLASWQAFPPA-----------EST-PEPLTRLQERLLRKLGANAHPFSFLIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KNL---D--EKIHKRNSVRLVIRKVQYAPEKP LVDPEYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLFLASWQAFPPA-----------EEA-PEPLTRLQERLLRKLGGNAHPFSFIIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KNV---D--EKIHKRNSVRLVIRKVQYAPEKP LVDTDYLKDRKVYVTLTCAFRYGR-EDLDVLGLSFRKDLFISKFQAYPPL-----------PEE-KKPLTRLQERLIKKLGEQAHPFFFTIPQNLPCSVTLQPGPEDTGK--ACGVDYEIRAFCA---KTM---E--EKMHKRNSVRLVIRKVQFAPEKP LVDADYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLFISTFQAYPPL-----------LEE-KKPLTRLQERLVKKLGEQAHPFYFTIPQNLPCSVTLQPGAEDTGK--ACGVDYEIRAFCA---KSM---E--EKMHKRNSVRLVIRKVQFAPEKT LIDPDYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLYISTFQAYPPQ-----------PEE-RRPLSRLQERLLKKLGQHAHPFYFTIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KSV---E--EKIHKRNSVRLVIRKVQYAPEKP LIDPEYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLYISTFQAFPAL-----------PEE-RRPLSRLQERLLKKLGQHAHPFYFTIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KSV---E--EKIHKRNSVRLVIRKVQYAPEKP LVDPEYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLYIFTFQAYPPI-----------PEE-SKPHSRLQERLLKKLGQNAYPFHFSIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KSM---E--EKNHKRNSVRLVIRKAQYAPEKP LIDPEYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLFISSFQAYPPL-----------PDE-RKPLSRLQERLLKKLGQNAYPFNFTIPQNLPCSVTLQPGPEDTGK--ACGVDFEVRAFCA---KTV---D--EKTHKRNSVRLVIRKVQYAPEKP LVDPEYLKDRKVFVTLTCAFRYGR-EDLDVLGLSFRKDLYISTFQAFPPV-----------PEE-RKPNSRLQDRLLKKLGQHAHPFYFTIPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KSI---E--EKIHKRNSVRLVIRKVQYAPEKP LVDPEYLKDRRVFVTLTCAFRYGR-EDLDVLGLSFRKDLFIATFQAFPPL-----------PEE-KKPLTRLQERLVKKLGENAFPFFFTLPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KTL---E--EKIHKRNSVRLVIRKVQYAPEKP LVDPEYLKDRRVFVTLTCAFRYGR-EDLDVLGLSFRKDLFIATFQAFPPL-----------PEE-KKPLTRLQERLIKKLGENAFPFFFMLPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCA---KAM---E--EKIHKRNSVRLVIRKVQYAPEKP ----------------------------------------------------------------------RLQERLVKKLGENAFPFFFTLPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCS---KTM---E--EKIHKRNSVRLVIRKVQYAPEKP ----------------------------------------------------------------------RLQERLVKKLGENAFPFFFTLPQNLPCSVTLQPGPEDTGK--ACGVDFEIRAFCS---KTM---E--EKIHKRNSVRLVIRKVQYAPEKP LIDPEYVKERKVFVKLLCAFRYGR-EDLDVLGLTFRKDFCTTTIQVYPPL-----------NEGDRKPKTRLQERLMKKLGEHSYPFFIDIPKDSPCSVTLQPAPGDTGK--PCGVDYELKTYVW---DPE---DNPDKTHKRNSVRLAIRKVQFAPDKK LIDPEYVKERKVFVKLLCAFRYGR-EDLDVLGLTFRKDFCTTTIQVYPPL-----------DEGDKK-KTRLQERLMKKLGEHAYPFFIEIPKDSPCSVTLQPAPGDTGK--PCGVDYELKTYVS---DGD---E--EKIHKRNSVRLAIRKVQFAPDKK LIDPEYVKGRKVYVKLLCAFRYGR-EDLDVLGLTFRKDFYTATKQVFPSE-----------TEEDKKPKTRLQERLMKKLGTNAYPFFIEIPANSPCSVTLQPANGDTGK--PCGVDYELKTYVG---DEP---E--EKLHKRNSVRLAIRKVQYAPDKQ LVDKEYLKDRKVFSHILAAFRYGR-EDLDVLGLTFRKDLYIASAQVYPPV-----------QET-QKPLTRLQERLIKKLGQNAYPFFFEMPKNAPSSVTLQPAPGDTGK--PCGVDYELKAFVA---DMS---D--EKPHKRNSVRLAIRKVTYAPSTP LVDKEYLKDRKVFSHILAAFRYGR-EDLDVLGLTFRKDLYIASAQVYPPQ-----------PSE-QKPLTRLQERLIKKLGQNAYPFFFEMPKNAPSSVTLQPAPGDTGKETPCGVDYELKAFVA---DSI---E--EKAHKRNSVRLAIRKVTYAPSTP LVDPEYLKDRKVFAHVLAAFRYGR-EDLDVLGLTFRKDLYLASMQVYPPI-----------TEE-QKPLTRLQERLIKKLGPNAHPFYFEVPKNSPSSVTLQPAPGDTGK--PCGVDYELKTYVA---DSI---D--EKPHKRNSVRLAIRKITYAPSTP FIDPEYVKDRKVFGQVLAAFRYGR-EDLDVLGLTFRKDLYLAHEQIYPPM------------QL-DRPMTRLQERLIKKLGPNAHPFYFEVPPYCPASVSLQPAPGDVGK--SCGVDYELKAFVG---ENV---E--DKPHKRNSVRLTIRKVMYAPSKV .......170.......180.......190.......200.......210.......220.......230.......240.......250.......260.......270.......280.......290.......300.......310.......320

188 184 185 185 201 225 193 197 189 177 180 180 177 177 179 178 178 179 180 180 183 183 159 203 203 203 203 203 203 176 190 179 178 179 175 178 178 178 178 178 178 178 178 178 177 177 175 175 177 176 176 178 178 178 178 178 178 178 178 190 190 213 178 200 182 171 220 178 178 178 158 124 114 192 153 179 178 12 113 19 179 179 179 179 188 182 254 200 179 179 179 175 179 179 179 179 179 80 80 183 180 179 207 209 202 218

Arr-S_Human_NP-000532 Arr-S_Cattle_XP-010802144 Arr-S_Tasmanian_devil_XP-003770202 Arr-S_Koala_XP-020827934 Arr-S_Chicken_XP-015132693 Arr-S_Ground-tit_XP-005529624 Arr-S_Golden_eagle_XP-011575693 Arr-S-Partial_Turtle_XP-007052695 Arr-S_Anole_XP-016847626 Arr-S_Gecko_XP-015282299 Arr-S_Xenopus_NP-989073 Arr-S_Nanorana_XP-018427475 Arr-S_Florida_gar_22184-1-1 Arr-S_Bowfin_24232-1-2 Arr-Sa_Zebrafish_NP-956853 Arr-Sa_Medaka_NP-001116373 Arr-Sb_Medaka_NP-001098559 Arr-S1_Reef_shark_55938-25-1 Arr-S1_Bamboo_shark_45077-7-1 Arr-S1_Whale_shark_XP-020370169-XP-020368728 Arr-S1_Bluespot_ray_30422-1-1 Arr-S1_Western_ray_26221-2-1 Arr-S1_Elephant_shark_XP-007902404 Arr-S2_Reef_shark_55253-10-3 Arr-S2_Bamboo_shark_28962-1-1 Arr-S2_Whale_shark_XP-020368730 Arr-S2_Bluespot_ray_31414-1-1 Arr-S2_Western_ray_22644-1-1 Arr-S2_Elephant_shark_XP-007887798 Arr-VZ_E.cirrhatus_129805-1-1 Arr-VY_G.australis_54008-1-2 Arr-VX_M.mordax_42250-9-1 Arr-VX_G.australis_59149-4-1 Arr-VX_L.camtschaticum_BAJ83617 Arr-C_Human_NP-004303 Arr-C_Cattle_NP-776419 Arr-C_Opossum_XP-007507671 Arr-C_Koala_XP-020832166 Arr-C_Chicken_NP-001091002 Arr-C_Ground-tit_XP-005526200 Arr-C_Golden_eagle_XP-011578691 Arr-C_Turtle_XP-007055341 Arr-C_Anole_XP-008122024 Arr-C_Gecko_XP-015269615 Arr-C_Xenopus_XP-012823333 Arr-C_Nanorana_XP-018410246 Arr-C_Florida_gar_15939-1-1 Arr-C_Bowfin_14016-1-1 Arr-Ca_Zebrafish_NP-001002405 Arr-Cb_Zebrafish_NP-957086 Arr-C_Medaka_XP-011478269 Arr-C-Partial_Reef_shark_104042-1-1w50893-1-1 Arr-C_Bamboo_shark_45371-8-2 Arr-C_Whale_shark_XP-020383069 Arr-C_Bluespot_ray_36257-4-1 Arr-C_Western_ray_37242-1-1 Arr-C_Elephant_shark_XP-007892403 Arr-B1_Human_NP-004032 Arr-B1_Cattle_NP-776668 Arr-B1_Tasmanian_devil_XP-003764705 Arr-B1_Koala_XP-020845203 Arr-B1_Golden_eagle_XP-011583127 Arr-B1_Ground-tit_XP-005524198 Arr-B1_Turtle_XP-006127564 Arr-B1_Gecko_XP-015265943 Arr-B1_Python_XP-007440235 Arr-B1_Xenopus_XP-004912283 Arr-B1_Florida_gar_28267-2-1w1-11 Arr-B1_Bowfin_26818-1-6 Arr-B1_Zebrafish_NP-001153294 Arr-B1_Medaka_XP-011471558 Arr-B1-Partial_Reef_shark_38868-1-2 Arr-B1-Partial_Bamboo_shark_c25388-1-1w2-1 Arr-B1_Whale_shark_XP-020371759 Arr-B1-Non-contig_Western_ray_78258-1-1w66643-1-1w83393-1-1 Arr-BY_M.mordax_41599-5-1 Arr-BY-Non-contig_G.australis_41931-1-1w37543-2-1w1-1w53942-1-1 Arr-BX-Partial_M.mordax_41599-4-1 Arr-BX-Partial_G.australis_54326-4-1w1-1w2-3 Arr-BX-Partial-Trimmed_P.marinus_ENSPMAP00000004285 Arr-BX_L.camtschaticum_BAJ83616 Arr-B2_Human_NP-004304 Arr-B2_Cattle_NP-001192206 Arr-B2_Tasmanian_devil_XP-003770754 Arr-B2_Koala_XP-020822087 Arr-B2_Turtle_XP-006116398 Arr-B2_Python_XP-007444595 Arr-B2_Gecko_XP-015283361 Arr-B2_Xenopus_NP-001093721 Arr-B2_Nanorana_XP-018417500 Arr-B2-Partial_Spotted_gar_XP-015195812 Arr-B2-Non-contig_Bowfin_13774-1-1w18902-1-1 Arr-B2a_Zebrafish_NP-999846 Arr-B2b_Zebrafish_NP-957418 Arr-B2_Medaka_XP-004076705 Arr-B2_Reef_shark_51008-1-2 Arr-B2_Bamboo_shark_31008-1-1 Arr-B2-Partial_Bluespot_ray_34441-5-1 Arr-B2-Partial_Western_ray_34309-2-1 Arr_C.intestinalis_NP-001041447 Arr_C.savignyi_ENSCSAVP00000014619 Arr_H.roretzi_BAC76419 Arr_B.floridae_XP-002593261 Arr_B.belcheri_XP-019616407 Arr_S.kowalevskii_NP-001171767 krz_D.melanogaster_NP-524988

. : : : * : * : : : : : :: * : : * :**: :. GPQPRAEAAWQFFMSDKPLHLAVSLNKEIYFHGEPIPVTVTVTNNTEKTVKKIKAFVEQ-VANVVLYSSDYYVKPVAMEEAQ-E--KVPPNSTLTKTLTLLPLL-ANNRERRGIALDGKIKHEDTNLASSTI-IKEGIDRTVLGILVSYQIKVKLTV-SGPQPRAEASWQFFMSDKPLRLAVSLSKEIYYHGEPIPVTVAVTNSTEKTVKKIKVLVEQ-VTNVVLYSSDYYIKTVAAEEAQ-E--KVPPNSSLTKTLTLVPLL-ANNRERRGIALDGKIKHEDTNLASSTI-IKEGIDKTVMGILVSYQIKVKLTV-SGPQPQVETAWQFFMSDKPLHLAISLSKEIFFHGEPIPVTVTVTNNTEKTVKKIKVQVEQ-VANVVLYSSDYYVKPVAMEEAN-E--KVPPNGVLTKTLTLTPLL-ANNRERRGIALDGKIKHEDTNLASSTI-IKEGIDRAVLGILVSYQIKVKLTV-SGPQPQMETAWQFFMSDKPLHLAISLSKEIYFHGEPIPVTVSVTNNTEKTVKKIKVLVEQ-VANVVLYSSDYYVKPVATEEAH-E--KVPPNGTLTKTLTLTPLL-ANNRERRGIALDGKIKHEDTNLASSTI-IKEGIDRTVLGILVAYQIKVKLTV-SGPQPCAETTWQFFMSNKPLHLKACLSKEVYYHGEPIPVTVMVTNSTEKTVKKIKVQVEQ-VANVVLYSSDFYTKVVAAEEAQ-E--KVQPNSSLTTTLTVLPLL-ANNRQTREIALDGKLKDEDTNLASSTI-IKDGIDKTVMGILVSYKVRVKLTV-SGPQPCAETTWQFFMSNKPLHLKACLSKEVYYHGEPIPVTITINNSTEKTVKKIKVQVEQ-VTNVVLYSSDFYTKVVASEEAR-E--KVQPNSSLTKTLTLLPLL-ANNRETREIALDGKLKDEDTNLASSTI-IKDGIDRTVMGILVSYKIKVKLTV-PGPQPCAETTWQFFMSNKPLHLKACLSKEVYYHGEPIPVTIIVNNSTEKTVKKIKVQVEQ-VANVVLYSSDYYTKVVAAEEAQ-E--KVQPNSSLTKTLTLLPLL-ANNRQTREIALDGKLKDEDTNLASSTI-IKDGIDKTVMGILVSYKVKVKLTV-PGPQPQAETTWQFFMSSKPLHLRARLSKEVFYHGEPIPVTVTVTNNTEKTVKKIRVLVEQ-VANVVLYSSDYYTKAVAMEELQ-E--KVQPNSTITKTLILLPLL-ANNRDKRGIALDGKLKDEDTNLASSTI-IKDGIDKTVLGILVAYKVKVKLIV-SGSQPQAETSWQFFLSKRPLHLKASLSKEVYYHGEAIPVTLTVINNTEKVVKKISISVEQ-VANVVLYCSDFYTKTVALEEVE-E--KIEPNCILTKTITVLPRL-ASNRTKKGIALDGKIKDEDANLASSTI-IKEGIDKTVMGILVAYKIKLKLTV-SGPQPQADTTWQFFLSKKPLHLKACLSKEVFYHGEPIPVTVTVINNTDKVVKKISVSVVQ-VANVVLYSSDFYTKTVALEEAE-V--KIQPNCTFIKTITILPLL-ANNREKRGIALDGKLKDEDTNLASSTI-IKDGIDKTVLGILVAYKIKVKLIV-SGPKPRAETSWQFFMSDKPLHLTASLPKEVFYHGEPISVSVSVTNNSDKTVKKISASVEQ-VSNVVLYSSDYYIKTVALEDSQ-D--KVPSKASYNHTFTLLPLL-AYNREKREIALDGKLKHEDTNLASSTL-LKEGIDRTVMGILVDYKIKVTLTV-SGPTPRAETSWQFFMSDKPLHMRAALTKEVFYHGEPISVAVTVTNKSDKNVKKISASVEQ-TANVVLYSSDFYTKTVAFDESN-E--KVPSKATYKHTFTLLPLL-AYNREKREIALDGKLKHEDTNLASSTL-LKEGTDRTVMGILVDYKIKVTLTV-SGPPPKVETTREFIMSDKPLHVEVSLEKETYYHGEPINVHVAINNSSNKTVKNITLTVEQ-VTNVVLYSNDKYVKPVAVEEPS-D--QVPSGTSLKKVYTLLPLL-ANNRERRGLSLDGKLKHEDTNLASSSI-VKEGVLKEILGILVAYRIVVKIIA-GGPPPKAETTREFLMSDKPLHVEVSLEKETYYHGEPINVHVNINNRSNKTVKSIVVSVEQ-IANVVLYSNDKYVKSVATEEID-D--HVPPGTSLKKVFTVLPLL-ANNRERRGLVLDGKLKHEDTNLASSSI-VREGVLKEILGILVAYRVVVKMQA-GGPAPSVETTRDFLMSDKPLHLEASLEKQTYYHGEPINVRVKINNQSNKNVRNIILSVEQ-NANVVLYCNDNYMKVVATEDSG-H--SVDSGAKLEKVYTLLPLL-ANNRERRGIALDGKLKHEDTNLASSSI-IKEGVQKEVLGIMVSYRVVVKLIV-GEVAPSVEITKDFVMSDKPLHVQATLDKELYYHGEPIKVHVNVTNNSNKNIKNIIVSVDQ-VATVVLFSNDSYTKCVDYEDNG-D--SVSAGATLKKVYTLLPLL-ANNRERRGIALDGKLKHEDTNLASSSV-VKEGVLKEVMGIMVSYRVMVKLIV-GELAPVAEMTFEFLMSEKPLQVKLSLPKETFYHGEVLKANVEITNSSSRNIKDISLSVEQ-VTNVVLYSNDKYVKSVAKEETD-D--SVPSGTTLKKEYTLHPLL-AYNKDRRGIALDGRLKHEDTNLASSSI-VKQEVLKEIQGMLVSYKVVLKMIA-SGPQPTANISKHFLMSNQPLHLTATLNKEVYYHGDPITVSVEVTNNSNKDVKGVRVSVVQ-STNVVLYSNDTYAEEIAVEESN-D--HVASGSKLSKSYVILPSL-TNNRSKHGIALDGRIKQEDSNLASSTI-LKEGADREVQGMLVSYVVKVSLSI-PGPQPSAHISKHFLMSDQPLHLTATLNKEVYCHGEPINVSIEVANNSNKDVKNVRLSVEQ-VTNVVLYSNDTYTEEVALEETN-D--QIASGSKFAQTYVLLPLM-ANNRDKHGIALDGRIKQEDTNLASSTI-LKEGADREVQGMLVSYKVKVSLLI-PGPQPSAHISKHFLMSDQPLHLTASLDKEVYYHGDPINVSIEATNNSNKDVKNVKLSVEQ-VTNVVLYSNDTYTKEVAVEEAN-D--LIASGSKFTKTYTLLPLM-ANNRDKHGIALDGRIKQEDTNLASSTI-LKEGTDREVQGMLVSYKVKVSLMI-PGSSPTAQISKHFLMSDQPLHLQASLNKEIYYHEEPINVSVQVTNNSSKHVKSIKIAVEQ-TTNVVLYSNDKYTEEVAAEEAS-D--QIAPGSTFSKTYVLLPLM-ANNRGKHGIALDGKIKQEGTNLASSTI-LKEGTDREVQGMLVSYTIKVSLLI-PGPQPTAQISKHFLMSDQPLHLQASLNKEIYYHGEPISVSIQVTNNSSKHVKSIKIAVEQ-ATNVVLYSNDKYIEEVAVEEVN-D--QIAPGSTFSKTYVLSPSM-ASNRGKHGIALDGKTKQEDTNLASSTI-LKEGSDREVQGMLVSYTMKVSLLI-PGPQPTAEISRHFLMSDDPLHLEASLDKETYYHGDPINVNIKVKNNSNKLAKGVKVSVEQ-LTQVVLYSKDKYVQEVAVEETD-E--QVAPSASLCKTYTLLPLA-ANNIEKHGIALDGKLKQEETNLASSTM-IKPGVAKDVLGMLVSYSVKVKLTI-PIFQPVAKITRQFLMSDKPLHLEASLNKEIFYHGEPVEVSVEVINHSTKTVKKIKVTADQ-IISVVLYSHDKYIQNVAEEEMD-E--QVTPGSTLKKIYTLYPII-ANNREKHGMALDGKIRDQDTNLASTTI-LKEGIDHEVWGILVSYRVKVKLVV-SIFQPVAKTTRQFLMSDKPLHLEASLNKEIYYHGEPVEVTVEVINHSTKTVKKVKITADQ-LASVVLYSHDKYSQTVAAEEVD-E--QVTPGSTLKKIYALYPVI-ANNRDKHGVVLDGKLRHEDTNLASTTI-LKEGIDREVWGILVSYKVRVKLIV-SIFRPVAKTTRQFLMSDKPLHLEASLNKEIYYHGEPVEVSVEVTNHSTKTVKKIKVTADQ-IASVLLYSHDKYSQTVATEEMD-E--QVAPGSTLKKVYTLYPII-ANNREKHGVVLDGKLRHEDTNLASTTI-LKEGIDREVWGILVSYKVRVKLIV-SIFQPMAKTSRQFLMSDKPLHLEAKLNKEIFYHGEPVQVSVEVINHSNKTVKRLKITANQ-ISSVVLYSHDKYSQTVAEQEAE-E--QISPGSTLNKVFSLYPII-ANNREKHGLALDGKLRHEDTNLASTTI-LKEGIDREVWGILVSYKVQVKLIV-SIFQPVARTSRQFLMSDKPLHLEASLNKEIFYHGEPVQVSVEVINHSNKTVKKLKITADQ-ISSVVLYSHDKYSETVAEQEVH-E--QVAPGSTLKKVYSLYPTI-ANNREKHGLALDGKIRHEDTNLASTTI-LKEGIDREVWGILVSYKVQVKLIV-STFHPMAKTIRHFLMSDEPIHLEVSLDKEIYYHNEPIQVSVDVMNYSNKTVKKIKITVEQ-IANVVLYSNDKYSQTLAMHEVN-E--QIAPGSKHKQEYTLLPLI-ENNREKHGVALDGKIKDEDTQLASSTI-LKEGIEREVWGILVSYKVKVKLVV-SGPPPAAN-----FMVDKSLSMNVTLPSQTFYHGESIPVNVHIDNQTKKIVKNITITVEQ-IANVVLYSHDKYVKTVLSEIFP-D--TVEPNSTLSRTFNVTPLR-ANNRSHRGIVLDGRIKHEDTNLASSTI-IKDPGKKETLGILVSYQLKVKASA-PGIIPSGSST---VDSDSPVTLAASLDKEVYYHGQPIRVSVSIKNGSSKHVKKMKITVDQ-ISDVVLYCQDKYTKTVCCEEPT-D--VVGPQGSLSAVYTVTPLA-AHNREKRGIALDGKLKHDDTNLASTTR-VSEGVDREVMGILVSYVIRVKALV-SVPPSRAETTRQFLMSDKPLQLDAALDKELYYHKEPIQVTVNINNSSSKHVKKIRISVEQ-VTEVILYSQDSYVKSVCAEEPS-D--TVPPSSSFSRVFSITPVMDASNRENRGMALDGKQKHVDTNLASSTI-LVEGMDKEVKAMLVSYLVRVKLTV-GLAAPRAETTRQFLMSDKPLQMDAALDKELYYHGEPIQVTVNINNSSSKHVKKIRITVEQ-ITDVVLYSQDKYAKSVCVEEPA-D--TLGPASTMSKVYSIVPMMGLNVREKRGIALDGKLKHEDTNLASSTL-LAEGLDKEVKGMLVTYQIRVKLTV-GVPPPRAETTRQFLMSDKPLQLEASLNKELYYHGEPIEVTVNINNGSSKHVKKVRLSVEQ-ITDIVLYSQDKYAKSVCVEEPT-D--TVGPASTYSKVYSIVPTMGVNVREKRGVALDGKLKHEDTNLASTTM-LVEGMDKEMLGMLVSYQIRVMLTV-GGPGPSAQTIRRFLLSAQPLQLQAWMDREVHYHGEPISVNVSINNCTNKVIKKIKISVDQ-ITDVVLYSLDKYTKTVFIQEFT-E--TVAANSSFSQSFAVTPIL-AASCQKRGLALDGKLKHEDTNLASSTI-IRPGMDKELLGILVSYKVRVNLMV-SGPGPWARLCRRFLLSAQPLLLQAWMDKEVNYHGQPISVNVSINNSTNKVIKKIKISVDQ-ITDVVLYSLDKYTKTVFVQEFT-E--TIAANSTFSKSFAVTPLL-ADNCHKQGLALDGKLKQGDTNLASSTI-LRPGVDKELLGILVSYKVRVNLMV-SGPKPRAETAWHFLLSDHPLKLEAWMDKEVYYHGEPISIHVAINNGSSKLIKKIKVAVDQ-IVDVVLYSGDKYTKTVFTAEFV-E--TVAANSTFSKSFTATPSL-AANRQKQGLALDGQLKHEDTNLASSTI-LQPGTDREALGILVSYQVRISMVV-SGPMPRAETTRHFLLSDHPLKLEAWMDKEVHYHGEPISIHVVICNGTSKLIKKIKVSVDQ-IVDVVLYSGDKYTKTVFTAEFG-E--TVPGHSTFSKSFTATPSL-AANRQKQGLALDGKLKHEDTNLASTTI-LQPGTDKEALGILVSYQVRISTVA-SGPGPKSETTRQFMMSDKPLHLEATLEKEIYYHGEPINVTVNINNTTNKIVKKIKIAVDQ-ITDVVLYSLDKYSKTVCMEEIN-E--NVAANSTFSKTYSVTPTL-SANREKRGLALDGKLKHEDTNLASTTI-LRPGMDKEVLGILVSYKVKVNLVV-SGPAPRAETTRQFMMSDKPLHLEASLDKEIYYHGDPINVTVNINNTTNKVVKKIKISVDQ-ITDVVLYSLDKYTKTVCSEEIN-E--TVAANSTFTKTYSVTPLL-SSNRQKRGLALDGKLKHEDTNLASTTI-LRPGMDKEVLGILVSYKVKVNLVV-SGPAPKSETTRQFMMSDKPLHLEASLDKEIYYHGDPINVTVNINNTTNKIVKKIKLSVDQ-ITDVVLYSLDKYTKTVCTEEIN-E--NVAANSTFSKTYSITPTL-SANREKRGLALDGKLKHEDTNLASTTI-LRPGMDKEVLGILVSYKVKVNLVV-SGPAPKSETTRQFMMSDKPLHLEASLDKEIYYHGDPITVNININNTTNKIVKKIKISVEQ-ITDVVLYSLDKYTKNVCTEEIL-E--TVAANSTFSKSYTVTPSL-SSNREKRGLALDGKLKHEDTNLASTTI-LRPGMDKEVLGILVSYKVKVNLVV-SGPAPKAETTRQFMMSDKPLHLEASLDKEVYYHGDPIGVNVSINNTTNKIVKKIKISVEQ-ITDVVLYSLDKYTKSVCSEEFL-E--NVAANSTFSKSYTVTPLL-SNNREKRGLALDGKLKHEDTNLASTTV-LRPGMDKEVLGILVSYKVKVNLMV-SGPAPKSETTKQFMMSDKPLHLEASLDKEIYYHGDPIGVNVKIINTTSKVVKKIKISVEQ-TTDVVLYSLDKYTKIVCSEEIT-E--NVAADSTLSKSYSVTPSL-TNNREKRGLALDGKLKHEDTNLASTTI-LRPGMDKEVLGILVSYKIRVNLMV-TGPAPCVQTTRQFMMSDRPLQLEASLNKEIYYHGEPIGVNVKITNNTSKIVKKIKITVEQ-LTDVVLYSLDKYTKIVCCEEMN-D--TVAANGTFSGSYSVTPLL-ANNREKRGLALDGKLKHGDTNLASSTI-LRPGMDKEVLGMLVSYKVRVNLVV-AGPGPHAEITRQFMMSDRPLQLEASLNKEIHYHGDPITVNVKINNSTNKIVKKIKITVEQ-VTDVVLYSLDKYTKIVCSDEMN-D--TVAANSAFTRSYQVTPLL-ANNTEKRGLALDGKLKHGDTNLASSTV-LRPGMDKEVLGILVSYKVRVNLMA-SGKGPKAETTRQFMMSDKPLHLEVSLDKEIFYHGDPIAVNIKINNETNKIVKKTKIGIEQ-ITDVVLYSLDKYSKGVLSEEFT-D--TVNGSSSFSKTYFITPLL-SNNREKRGLALDGKLKDQDTNLASTTI-LRPGVDKEVLGILVSYKLKVNLMV-SGTVLKADTTRQFMMSDKPLRLEASMTKEVHYHGEAIGVTIKINNETNKIVKKVKVTIEQ-LTDVVLYSLDKYTKIVLNEEFA-D--TVNGNSTFEKTYNVTPLL-TNNREKRGLALDGKLKHEDTNLASTTL-LRPGLDKEVLGILVSYKLKVNLMV-SGSGQKAELCKSFMMSDKPVLLEASLEKEIYYHGDPIPVNIKIKNETNKVVKRIRVTVDQ-TTDVVLYSADKYTKNVLTEEFG-E--TVEANSSFEKSLDITPLL-ANNKEKRGLALDGRLKDEDTNLASTTI-IRTGMDKEMLGILVSYKIKVNLMV-SAAGPKADINKQFITADKPIHMEVSMEKELYYHGDPIPIKVKVNNETSKVVKKIKINIFQ-ITDVVIYAADKYHKCVLNEEFG-D--QINANSTFEKEYSVTPLL-VNNKEKRGLALDGRLKDEDTNLASSTL-LIPDMDKQMQGVVVSYKIKVILMM-GKAGPKAEISKQFMMTDKPVNVEISLEKEIYYHGDPISVNVKINNETTKTVKKIKVSVEQ-LTSVVLYSSDTYSKEVCAQEFG-E--TVGANSTFEKSFQVVPLL-ADNKDKRGLAVDGRLKDEDTNLASTTL-GQG--ERELQGILVSYKVKVTVTV-STVKPKAETTRQFLMSDKPLQLEASLGKETYYHGEPIKVTVHINNNTSKVVKKIKLCVDQ-TTNVVLYQLDKYTKTICSEEPT-D--TVAPNSTFNKVYSLTPTL-EGNRQKQGLALDGKLKHEDTNLASSTM-LRAGLDKEVLGMIVSYLLKVKVTV-STVQPKAEITRQFLMSDKPLQLEALLGKEMYYHGEPIQVTVHINNNTSKVVKKIKLSVDQ-ITDVVLYQLDKYAKTVCSEEPN-D--TVAPNSTFTKVYSLTPTL-ANNRQKRGLALDGKLKHQDTNLASSTV-LRPQLDKEVLGILVSYLLRVKVIV-STAQPKAEITRQFLMSDKPLQLEASLDKEMYYHGEPLKVTVHINNNTSKVVKKIKLSVDQ-ITDVVLYQLDKYAKMICSEEPN-D--TVAPNSTFTKVYSLTPTL-ANNRQKRGLALDGKLKHQDTNLASSTI-LSPQLDKEVLGILVSYLLRVKVIV-SSLQPMSEATRQFLMSDKPLHLKASLSKEIYYHDEPIDVTVCITNNTSKVVKKIKLSVDQ-VTDVVLYQLDKYTKTVAVEESD-D--TVAANSSFTKVYKLTPVL-ANNRKRRGLALDGKLKHEDTNLASSTM-LRPELDKEVLGILVSYLMKVKVVA-SSLQPKAEATRQFLMSDKPLYLEASLGKEMYYHNEPIDVTVCITNNTSKVVKKIKLSVDQ-VTDVVLYQLDKYTKTVAFEEPD-D--TVAANSNFTKVYKLTPGL-ANNRKKRGLALDGKLKHEDTNLASSTM-LRPELDKEVLGILVSYLMKVKVIV-SKVKPKVETTRQFLMSDKPLNLQVSLEKELFYHEEPIKITVHINNTTNKVVKKIKLWVEQ-ITEVVLYQLDKYAKVVSSEETD-D--TVAVNSTFTKVYSLTPIL-ESNRQKRGLALDGKLKHQDTNLASSTM-LRPELDKDVLGILVSYCLKVKVTV-SGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAMEEAD-D--TVAPSSTFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LREGANREILGIIVSYKVKVKLVV-SGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAMEEAD-D--TVAPSSTFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LREGANREILGIIVSYKVKVKLVV-SGPQPTAETTRHFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAVEEAD-D--MVAPSSTFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGTNKEILGIIVSYKVKVKLVV-SGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAVEEAD-D--MVAPSSTFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTLRLRDGTNKEILGIIVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAVEDAD-D--MVAPSSTFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGANKEILGIIVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAVEDAD-D--MVAPSSTFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGANKEILGIIVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAVEEAD-D--MVAPSSTFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGANKEILGIIVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAVEESD-D--VVAPSSTFCKVYTLTPFL-ASNREKRGLALDGKLKHEDTNLASSTL-LRDGANKEILGIIVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAVEEAD-D--VVAPSSTFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGANKEVLGIIVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAVEEADND--VVAPSSTFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGANKEILGIIVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVATEESD-D--IVAPSATFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LREGANKEILGIIVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVATEESD-D--VVAPSATFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LREGANKEILGIIVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKKIKISVRQ-YADICLFNTAQYKCPVAIEESD-D--IVAPSATFCKVYTLTPFL-ANNREKRGLALDGKLKHEDTNLASSTL-LREGANKEILGIIVSYKVKVKLVV-SGPQPTAETTRQFLMSDKPLHLEASLDKEIYYHGEPISVNVHVTNNTNKTVKRMKISVRQ-HADICLFNTAQYKCPVAVEESD-D--VVAPSSTFCKVFTLTPFL-ANNQEKRGLALDGKLKHEDTNLASSTL-LRDGANKEILGILVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKLKIAVRQ-YADICLFNTAQYKCPVATAEAD-D--IVAPSSTFCKVYTLTPYL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGASKEILGIMVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKLKIAVRQ-YADICLFNTAQYKCPVATAEAD-D--IVAPSSTFCKVYTLTPYL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGASKEILGIMVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKLKIAVRQ-YADICLFNTAQYKCPVATAEAD-D--IVAPSSTFCKVYTLTPYL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGASKEILGIMVSYKVKVKLVV-SGPQPMAETTRQFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNTNKTVKKLKIAVRQ-YADICLFNTAQYKCPVATAEAD-D--IVAPSSTFCKVYTLTPYL-ANNREKRGLALDGKLKHEDTNLASSTL-LRDGANKEILGIIVSYKVKVKLVV-SGPQPTAETTRVFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNSNKTVKKIKISVRQ-FADICLFNTAQYKCPVAVEEAD-D--TVAPSSTFCKVYTLTPFL-ASNREKRGLALDGKLKHEDTNLASSTI-VKEGANKEILGILVSYKVKVKLVV-SGPQPTAETTRVFLMSDKPLHLEASLDKEIYYHGEPINVNVHVTNNSNKTVKKLKISVRQ-FADICLFNTAQYKCPVAVEEAD-D--TVAPSSTFCKVYTLTPFL-ASNREKRGLALDGKLKHXXXXXXXXXX-XXXXXXXXXXXXXXXXXXXXXXXX-XGPQPMAETTRQFLMSDKPLHLEASLDKMIYYHGEPINVNVHVTNNSTKTVKKIKMTVRQ-FADICLFNTAQYKCPVATEDGD-D--AVGASSTLCKVYTLTPLL-ASNRDKRGLALDGKLKHEDTNLASSTI-VQEGANKEVLGILVSYRVKVKLIV-SGPQPMAETTRQFLMSDKPLHLEASLDKMIYYHGEPINVNVHVTNNSTKTVKKIKMTVRQ-FADICLFNTAQYKCPVASEDGD-D--AVGPSSTLCKVYTLTPLL-ASNRDKRGLALDGKLKHEDTNLASSTI-VQEGANKEVLGILVSYRVKVKLIV-SGPQPMAETTRQFLMSDKPLHLEASLDKMIYYHGEPINVNVHVTNNSTKTVKKIKMTVKEFFADICLFNTAQYKCPVATEEGD-D--TVGPSSTLCKVYTLTPLL-ASNRDKRGLALDGKLKHEDTNLASSTI-VQDGANKEVLGILVSYRVKVKLIV-SGPQPMAETTRQFLMSDKPLHLEASLDKMIYYHGEPINVNVHVTNNSTKTVKKIKMTVRQ-FADICLFNTAQYKCPVATEEGD-D--TVGPSSTLCKVYTLTPLL-ASNRDKRGLALDGKLKHEDTNLASSTI-VQDGANKEVLGILVSYRVKVKLIV-SGPQPSAETTRHFLMSDRSLHLEASLDKELYYHGEPLNVNVHVTNNSTKTVKKIKVSVRQ-YADICLFSTAQYKCPVAQLEQD-D--QVSPSSTFCKVYTITPLL-SDNREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLGILVSYRVKVKLVV-SGPQPSAETTRHFLMSDRSLHLEASLDKELYYHGEPLNVNVHVTNNSTKTVKKIKVSVRQ-YADICLFSTAQYKCPVAQVEQD-D--QVSPSSTFCKVYTITPLL-SNNREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLGILVSYRVKVKLVV-SGPQPTAETARHFLMSDRSLHLEASLDKELYYHGEPLSVNVHVTNNSTKTVKKIKVSVRQ-YADICLFSTAQYKCPVALIEQD-D--QVSPSSTFCKVYNITPLL-SENREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLGILVSYRVKVKLVV-SGPQPTAETARHFLMSDRSLHLEASLDKELYYHGEPLSVNVHVTNNSTKTVKKIKVSVRQ-YADICLFSTAQYKCPVALIEQD-D--QVSPSSTFCKVYNITPLL-SENREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLGILVSYRVKVKLVV-SGPQPMAETTRHFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSSKSVKKIKVSVRQ-YADICLFSTAQYKCPVAQIEQD-D--QVSPSSTFCKVYTLTPLL-SQNREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLGILVSYRVKVKLVV-SGPQPTAETTRHFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSSKSVKKVKVAVRQ-YADICLFSTAQYKCPVAQIEQE-D--QVAPSSTFCKVYTLTPLL-SNNREKRGLALDGKLKHEDTNLASSTI-VTEGTSKEVLGVLVSYRVKVKLVV-SGPQPMAETTRHFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSSKSVKKVKVSVRQ-YADICLFSTAQYKCPVAQIELD-D--QVAASSTFCKVYTLTPLL-SNNREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLG--VSYRVKVKLVV-SGPQPVAETTRHFLMSDRSLHLEASLDKELYYHGEPINVNVHVTNNSSKTVKRVKVSVRQ-YADICLFSTAQYKCPVAQIEQD-D--QVTPSSTFCKVYTLTPLL-SNNREKRGLALDGKLKHEDTNLASSTI-VKEGSSKEVLGILVSYRVKVKLVV-SGPQPIAETTRNFLMSDRSLHLEASLDKELYYHGEAINVNVHVTNNSGKTVKRVKVSVRQ-YADICLFSTAQYKCPVAHIEQD-D--QVTPSSTFCKVYTLTPLL-SNNREKRGLALDGKLKHEDTNLASSTI-VKEGSSKEVLGILVSYRVKVKLVV-SGPQPMVETTRSFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSTKTVKKVKISVRQ-YADICLFSTAQYKCPVAQVEAD-D--QVSPSSTFCKVYTLTPTL-NNNREKRGLALDGKLKHEDTNLASSTI-VKEPANKEVLGILVSYRVKVKLVV-SGPQPMVETTRSFLMSDRSLHLEASLDKELYYHGEPXXXXXXVTNNSTKTVKKIKISVRQ-YADICLFSTAQYKCPVAQVEAD-D--QVSPSSTFCKVYTLTPML-SNNREKRGLALDGKLKHEDTNLASSTI-VKEVANKEVLGILVSYRVKVKLVV-SGPQPMVETTRSFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSTKTVKRVKISVRQ-YADICLFSTAQYKCPVAQIEAD-D--QVASSSTFCKVYTLTPTL-NNNREKRGPALDGKLKHEDTNLASSTI-VKDVSNKEVLGVLVSYRVKVKLVV-SGPQPMVETTRSFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSTKTVKRVKISVRQ-YADICLFSTAQYKCPVAQVEAD-D--QVSSSSTFCKVYTLTPTL-SNNREKRGLALDGQLKHEDTNLASSTI-VKDVSNKEVLGILVSYRVKVKLVV-SGPQPMVETTRSFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSTKTVKRVKISVRQ-YADICLFSTAQYKCPVAQLEAD-D--QVSSSSTFCKVYTLTPTL-DKNREKRGLALDGKLKHEDTNLASSTI-VKDVTNKEVLGILVSYRVKVKLVV-SGPQPMAETTRNFLMSDRSLHLEASLDKELYYHAEPISVNVHVTNNSTKTVKKIKISVRQ-YADICLFSTAQYKCPVAQVEAD-D--QVSASSTFCKVYTLVPLL-DHNREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLGILVSYRVKVKLVV-SGPQPMAETTRNFLMSDRSLHLEASLDKELYYHAEPISVNVHVTNNSTKTVKKIKISVRQ-YADICLFSTAQYKCPVAQVEAD-D--QVSASSTFCKVYTLVPLL-DHNREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLGILVSYRVKVKLVV-SGPQPMAETTRNFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSTKTVKKIKISVRQ-YADICLFSTAQYKCPVAQLEAE-D--QVSASSTFCKVYTLVPLL-DHNREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLGILVSYRVKVKLVV-SGPQPMAETTRNFLMSDRSLHLEASLDKELYYHGEPISVNVHVTNNSTKTVKKIKISVRQ-YADICLFSTAQYKCPVAQLEAE-D--QVSASSTFCKVYTLVPLL-DHNREKRGLALDGKLKHEDTNLASSTI-VKEGANKEVLGILVSYRVKVKLVV-SGPQPTAEIQKLFLMSDKPLCLEATLDKDLYYHGESIGVNVQVTNNSSKTVKKVKISVRQ-IADICLFSNAQYKCPVAFIEAD-D--QVAPSSTLCKVYQICPLL-GDNKDKRGLALDGQLKHEDTNLASSTI-LKEGVPKENLGIIVQYKVKVKLVLGSGPQPTAEVQKQFLMSDKPLCLEATLDKDLYYHGESIGVNVQVTNNSSKTVKKVKISVRQ-IADICLFSNAQYKCPVAVLEAD-D--QVAPSSTLCKVYQICPLL-GENKDKRGLALDGQLKHEDTNLASSTI-LKEGVLKENLGIIVQYKVKVKLVLGSGPQPSAEVRKDFMMSEKPLCLEASLDKELYYHGEKINVNVQVTNNSSKTVKKVKISVRQ-FADICLFSSAQYKCPVASIEAD-D--TVSPSSTLCKVYQICPLL-SSNKTKRGLALDGQLKHEDTNLASSTI-MKDGVPKENLGIIVQYKVKVKLVIGGGPQPFAEASREFMMSANPLHLECSLDKELYYHGEPINVNVQITNNSNKTVKKIRISVRQ-FADICLFSTAQYKCPVAILETE-DGFPVGPSQTLSKVYTVCPLL-KNNLDKRGLALDGKLKHEDTNLASSTI---------------------------GPQPFAEASREFMMSANPLHLECSLDKELYYHGEPINVNVQITNNSNKTVKKIRISVRQ-FADICLFSTAQYKCPVAILETE-DGFPVGPSQTLSKVYTVCPLL-KNNLDKRGLALDGKLKHEDTNLASSTI-MTKDTTRENLGIVVQYKVKIRLIV-AGPQPSVEASKEFMMSTAPLHLESSLDKEMYYHGESIAVNVQITNNSNKTVKKIRISVRQ-FADICLFSTAQYKCPVAVLETE-DGFPVGPSQTLSKVYYVTPLL-ANNRDKRGLALDGKLKHEDTNLASSTI-ITDESQKENLGIIVQYKVKVRLIV-AGEQPSIEVSKEFMMKPNKIHLEATLDKELYHHGEKISVNVHVANNSNRTVKKIKVCVRQ-FADICLFSTAQYKSVVAEIESE-DGCQVAPGFTLSKVFELCPLL-ANNKDKWGLALDGQLKHEDTNLASSTL-ITNPAQRESLGIMVHYKVKVKLLISSP 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Arr-S_Human_NP-000532 Arr-S_Cattle_XP-010802144 Arr-S_Tasmanian_devil_XP-003770202 Arr-S_Koala_XP-020827934 Arr-S_Chicken_XP-015132693 Arr-S_Ground-tit_XP-005529624 Arr-S_Golden_eagle_XP-011575693 Arr-S-Partial_Turtle_XP-007052695 Arr-S_Anole_XP-016847626 Arr-S_Gecko_XP-015282299 Arr-S_Xenopus_NP-989073 Arr-S_Nanorana_XP-018427475 Arr-S_Florida_gar_22184-1-1 Arr-S_Bowfin_24232-1-2 Arr-Sa_Zebrafish_NP-956853 Arr-Sa_Medaka_NP-001116373 Arr-Sb_Medaka_NP-001098559 Arr-S1_Reef_shark_55938-25-1 Arr-S1_Bamboo_shark_45077-7-1 Arr-S1_Whale_shark_XP-020370169-XP-020368728 Arr-S1_Bluespot_ray_30422-1-1 Arr-S1_Western_ray_26221-2-1 Arr-S1_Elephant_shark_XP-007902404 Arr-S2_Reef_shark_55253-10-3 Arr-S2_Bamboo_shark_28962-1-1 Arr-S2_Whale_shark_XP-020368730 Arr-S2_Bluespot_ray_31414-1-1 Arr-S2_Western_ray_22644-1-1 Arr-S2_Elephant_shark_XP-007887798 Arr-VZ_E.cirrhatus_129805-1-1 Arr-VY_G.australis_54008-1-2 Arr-VX_M.mordax_42250-9-1 Arr-VX_G.australis_59149-4-1 Arr-VX_L.camtschaticum_BAJ83617 Arr-C_Human_NP-004303 Arr-C_Cattle_NP-776419 Arr-C_Opossum_XP-007507671 Arr-C_Koala_XP-020832166 Arr-C_Chicken_NP-001091002 Arr-C_Ground-tit_XP-005526200 Arr-C_Golden_eagle_XP-011578691 Arr-C_Turtle_XP-007055341 Arr-C_Anole_XP-008122024 Arr-C_Gecko_XP-015269615 Arr-C_Xenopus_XP-012823333 Arr-C_Nanorana_XP-018410246 Arr-C_Florida_gar_15939-1-1 Arr-C_Bowfin_14016-1-1 Arr-Ca_Zebrafish_NP-001002405 Arr-Cb_Zebrafish_NP-957086 Arr-C_Medaka_XP-011478269 Arr-C-Partial_Reef_shark_104042-1-1w50893-1-1 Arr-C_Bamboo_shark_45371-8-2 Arr-C_Whale_shark_XP-020383069 Arr-C_Bluespot_ray_36257-4-1 Arr-C_Western_ray_37242-1-1 Arr-C_Elephant_shark_XP-007892403 Arr-B1_Human_NP-004032 Arr-B1_Cattle_NP-776668 Arr-B1_Tasmanian_devil_XP-003764705 Arr-B1_Koala_XP-020845203 Arr-B1_Golden_eagle_XP-011583127 Arr-B1_Ground-tit_XP-005524198 Arr-B1_Turtle_XP-006127564 Arr-B1_Gecko_XP-015265943 Arr-B1_Python_XP-007440235 Arr-B1_Xenopus_XP-004912283 Arr-B1_Florida_gar_28267-2-1w1-11 Arr-B1_Bowfin_26818-1-6 Arr-B1_Zebrafish_NP-001153294 Arr-B1_Medaka_XP-011471558 Arr-B1-Partial_Reef_shark_38868-1-2 Arr-B1-Partial_Bamboo_shark_c25388-1-1w2-1 Arr-B1_Whale_shark_XP-020371759 Arr-B1-Non-contig_Western_ray_78258-1-1w66643-1-1w83393-1-1 Arr-BY_M.mordax_41599-5-1 Arr-BY-Non-contig_G.australis_41931-1-1w37543-2-1w1-1w53942-1-1 Arr-BX-Partial_M.mordax_41599-4-1 Arr-BX-Partial_G.australis_54326-4-1w1-1w2-3 Arr-BX-Partial-Trimmed_P.marinus_ENSPMAP00000004285 Arr-BX_L.camtschaticum_BAJ83616 Arr-B2_Human_NP-004304 Arr-B2_Cattle_NP-001192206 Arr-B2_Tasmanian_devil_XP-003770754 Arr-B2_Koala_XP-020822087 Arr-B2_Turtle_XP-006116398 Arr-B2_Python_XP-007444595 Arr-B2_Gecko_XP-015283361 Arr-B2_Xenopus_NP-001093721 Arr-B2_Nanorana_XP-018417500 Arr-B2-Partial_Spotted_gar_XP-015195812 Arr-B2-Non-contig_Bowfin_13774-1-1w18902-1-1 Arr-B2a_Zebrafish_NP-999846 Arr-B2b_Zebrafish_NP-957418 Arr-B2_Medaka_XP-004076705 Arr-B2_Reef_shark_51008-1-2 Arr-B2_Bamboo_shark_31008-1-1 Arr-B2-Partial_Bluespot_ray_34441-5-1 Arr-B2-Partial_Western_ray_34309-2-1 Arr_C.intestinalis_NP-001041447 Arr_C.savignyi_ENSCSAVP00000014619 Arr_H.roretzi_BAC76419 Arr_B.floridae_XP-002593261 Arr_B.belcheri_XP-019616407 Arr_S.kowalevskii_NP-001171767 krz_D.melanogaster_NP-524988

---GFLGELTSSEVATEVPFRLMHP--QPEDP--AK-----ES------------------------YQ------------------------------------------DANLVFEEFARH--NLKDAGEAEEGKRDKND--VDE--------GLLGELTSSEVATEVPFRLMHP--QPEDPDTAK-----ES------------------------FQ------------------------------------------DENFVFEEFARQ--NLKDAGEYKEEKTDQEAA-MDE--------GFLGDLTSSEVGTDLPFRLMHP--QPVDP--AK-----DS------------------------ST------------------------------------------GEDLVFEEFARQ--NLKDGAENEEEKKEGPE--NDE--------GFLGDLTSSEVCTDLPFRLMHP--QPADP--AK-----ES------------------------SM------------------------------------------GEDLVFEEFARQ--NLKDIADNEEEKKDKEARVTDD--------GLLGDFTSSEVSTELPFRLMHP--KPEE---KN-----SA------------------------GKD----------------------------------------GDAEMVFEEFARQ--QLKDTGDEEENKNVSS---TDE--------GMLGDLTSSEVGTELPFRLMHP--KPEE---KN-----PA------------------------GKD----------------------------------------GEAELVFEEFGRQ--QLKNTPD-EEDKNSPS---TDE--------GMLGDLTSSEVGTELPFRLMHP--KPEE---KN-----TA------------------------GKD----------------------------------------GEAELVFEEFARQ--QLKDMLD-DEDKNMPS---TDE--------GMLGDLTSSEVSTELPFRLMHP--KPEE----------------------------------------------------------------------------------------------------------------------------GLL-DFVSSEVSMELPFRLMHP--KREE---IN-----PT------------------------GK------------------------------------------ESDLVFEDFARQ--QLNDENSEEDDKADPS---NDD--------GLLGDFTSSDVGIELPFRLMHP--KPEE---VN-----AT------------------------GQ--------------------------------------------------------------------------------------GLLGDMTSSEVSTELPFILMHP--NPES---AK-----ES------------------------EQ------------------------------------------DDDMVFEEFARD--QLKDELQAEEKEEEED---DEK--------GLLGDMTSSEVSTELPFILMHP--KPDNG--AK-----ES------------------------EQ------------------------------------------EEDLVFEDFARD--QLKDELKGEDKEEEDE---DEK--------GIVGDMGASEIGLELPFRLMHP--KPES---VK-----ES------------------------EL------------------------------------------EEDMVFEEFKRS--YLKGMV---EDEEEGNISPAE---------GIVGDFGASEIGVEVPFQLMHP--KPDI---VK-----ES------------------------EQ------------------------------------------EDEMVFEEFKRS--YLKGMV---EDEEDANLSATEG--------GM---MGSSEVGVELPFQLMHP--KPDA---VK-----ES------------------------EA------------------------------------------EEEMVFEEFKRT--YLKGMP---EDEDEGNANIEENV-------GM---MGSSEVGLEVPFRLMHP--KPDA---VR-----ES------------------------EM------------------------------------------EDEMVFEEFKRS--YLRGIIAGDDDDEEGNVSGGDDITPKEK ---GM---VGSSEVSLEVPFKLMHS--KPEA---AK-----ES------------------------E-------------------------------------------PDDMVFEDFKRD--YLKGVVYGDDDESPAEL-------------GVLGDLTASDVAVEIPFLLMHP--NPEA---TK-----KP------------------------D-------------------------------------------DEDIAIEDEADL--SP---LEAEDDE------------------GVLGDLTASDATVEIPFLLMHP--NPES---IK-----NP------------------------E-------------------------------------------EEDIAIEDDGDV--SP---ADDEDDE------------------GVLGDLTASDATVEIPFLLMHP--NPES---IK-----NP------------------------E-------------------------------------------EEDIAIEDDGDL--SP---ADAEDDE------------------GMLGDITSSDATVEIPFLLMHP--SPEN---NK-----NP------------------------E-------------------------------------------DDDFAIEEASGL--SPQETADAEDDD------------------GMLGDITSSDVTMEIPFLLMHP--NPES---NK-----NQ------------------------E-------------------------------------------DDDFAIEEGGEL--SPQGTVDAEDDD------------------GVLGDLTSSDVIVELPFMLMNP--NPDT---SK-----DE------------------------E-------------------------------------------DNEMAIEDCPDV--QDAADGEADDD-------------------GMLGDVLFSDVAVELPFLLMQP--KPLS---ES-----LD------------------------D-------------------------------------------DDDIQFEDFDQG--GPMAVTEDLKEGAESLFKSSNACH------GMLGDVLFSDVAVELPFLLMHP--NPQT---GS-----LV------------------------D-------------------------------------------QDDIEFEDFDRG--GPMAVTEDLKEGVETVYRTSAASS------GMLGDVLFSDIAVDLPFLLMNP--KPQT---ES-----LI------------------------D-------------------------------------------HDDIEFEDFDRG--GPMAVTEDLKEGSESPFSNSVASK------GMLGDVLFSDVAVELPFMLMHPPREHSA---ES-----DA------------------------D-------------------------------------------GDDIDFEDFDCR--GPMAVTEDLKEGQESDSSVP----------GMLGDVLFSDAVVELPFLLMHPPREASA---EN-----LA------------------------D-------------------------------------------DDDIDFEDFDRG--GPMAVTEDLKEGKESNSHSMMCSSASH---GMLGDIMFSDVAVEIPFLLMNP--NPQT---QTGKYLVEK------------------------D-------------------------------------------DDEIQFEDFGQR--RPMAVTEDLKDGEESP------------RSG--------DLAVMLPFLLMHP--APKE---QEAQSLTTL------------------------E-------------------------------------------SKNIVLEEFGRK--AISRAELEDEEGEDEPN--EGD------RNGILGDIAGSDLLVELPFTLMHP--TPME---DIVYSEVSQ------------------------R-------------------------------------------GVDIVVEDFARK-----GEEVKEEEAEDEAE--QP-------RGGILGDLTTSEVFVDLPFTLTHP--KPVE---SL-------------------------------G-------------------------------------------DEDIVIEDFVRK--TCDGKD--AEEEEAQAE--G--------RGGILGDLAASEVFVDVPFRLMHP--KPVE---GV-------------------------------K-------------------------------------------EGDIIIEDFARS--TCDGKKEVEEEEEAQAE--G--------RGGILGDLASSEVFVDLPFTLMHP--KPVE---GI-------------------------------K-------------------------------------------EGDIIIEDFARN--TCDGKKEVEDEEAAEAE--G--------CGGILGDLTASDVGVELPLVLIHP--KPSH---EA-----A-------------------------S-------------------------------------------SEDIVIEEFTRK--GEE-ESQKAVEAEGDE----GS------CEGILGDLTASEVGVELPLILMHP--KPSN---EA-----A-------------------------S-------------------------------------------SEDIVIEEFAQQ--EPSGESQEALAAEGNE----GS------RGGILGDLMSSDMSVALPLRLMHP--KPSC---DV-----T---------------------------------------------------------------------SEDVVIEDFA-------GHDTEEETTVATASGAKDN------RGGILGDLTSSDVSVVLPLSLMHP--KPSS---DV-----T-------------------------R-------------------------------------------SEDVVIEDFSQQWPKIQGADETEEEATSTASGTEDN------RGGILGDLTSSDVGVELPIILMHP--KPAD---DK-----PR------------------------S-------------------------------------------EEDIVIEEFARQ--KLKGEKDDEDEKEEGEK--EES------RGGILGDLTASDVGVEMPVILMHP--KPDD---SK-----PR------------------------S-------------------------------------------EEDIVIEEFARQ--KLKGEKDDEDEKEEPEK--EES------RGGILGDLTASDVGVELPVILMHP--KPAD---DK-----PR------------------------S-------------------------------------------EEDIVIEEFARQ--KLKGEKDDEE--EEANK--EES------RGGILGDLTSSDVGVEMPVILMHP--KPTD---G--------------------------------D-------------------------------------------EEDIVIEEFARQ--KLQGEKDDEDEKEEAEK--DES------RGGILGDLTSSDVGVEVPVILMHP--KPTD---NT-----P-------------------------S-------------------------------------------AEDIVIEEFARQ--KLKGEKDDDEEKEEEDK--GES------RGGILGDLTSSDIGVDLPLVLMHP--KPTD---ST-----P-------------------------S-------------------------------------------EEDIVIEEFARQ--KIKGEPDDEEEKEEEDK--EES------RGGILGDLTSSDVLVDLPLTLMHP--KPSP---DQ-----T-------------------------N-------------------------------------------IEDVVIEEFARQ--KLQGAEGEDDKEDA--------------RGGILGDLISSDVAVELPLVLMHP--KPPE---GT-----T-------------------------S-------------------------------------------AEDVVIEEFARQ--KLQGEQDDEEDKEEER------------RGGILGDLTASDVGVELPFTLMHP--KPSE---SV-------------------------------S-------------------------------------------QEDIVIEEFARQ--KLKGEKDDEEEKEDQEE--K--------RGGILGDLTASDVGVELPLTLMHP--KPVE---PV-------------------------------S-------------------------------------------QEDIVIEEFARQ--KLKGEKDDEEEQEEKE------------GGGLLGGLTASDVTVELPLTLMHP--KPKD---------------------------------------------------------------------------------------------------------------------------GGLLGSLTSSDVTAELPLVLMSP--KPAE---IS-----------------------------------------------------------------------------DINLE-----------------------------------GGGMLGGLTGSDVVVEVPVTLMSP--KPAE---VT-----------------------------------------------------------------------------DVNME---------------------PAE--G--------RGGILGDLTSSEVSVEIPFYLMHP--KPSE---DN------V------------------------S-------------------------------------------AEDIVIKEFNRQ--KIDGEQDDEDEGKAATE--GEK------RGGILGDLTSSEVSVEIPFYLMHP--KPSE---VN------V------------------------C-------------------------------------------AGDIVIEEFNRP--KLDEEQDDEDEEHAATE--GEN------RGGILGDLTSSEVSVEIPFCLMHP--KPLE---VN------V------------------------C-------------------------------------------TEDIVIEEFNRP--KLDEEQDDEDEEPAAAE--GEN------RGGILGDLTSSEIMVEIPFYLMHP--KPSD---VN------V------------------------S-------------------------------------------VGDIVVEDFNRQ--KVDGERDDEEEEMAANE--GED------RGGVLGDLTSSEVVVEIPFYLMHP--KPSE---VN------A------------------------S-------------------------------------------TKDLVIEEFNRQ--KIDGEKDDEEEETAATE--GEH------RGGILGDLTASDVMVDIPISLMHS--KPSE---L-------T------------------------S-------------------------------------------MEDIVIEEFSRH--KIKGEKDDDEEEEAAAG--QES------RGGLLGDLASSDVAVELPFTLMHP--KPKE---EP-----PH------------------------RE--------------VPENETPVDTNLIELDTN----------DDDIVFEDFARQ--RLKGMKDDKEEEE-DGTGSPQLNNR---RGGLLGDLASSDVAVELPFTLMHP--KPKE---EP-----PH------------------------RE--------------VPEHETPVDTNLIELDTN----------DDDIVFEDFARQ--RLKGMKDDKEEEE-DGTGSPRLNDR---RGGLLGDLASSDVAVELPFTLMHP--KPRE---EP-----VR------------------------RE--------------VPENEAPIDTNLIELDTN----------DDDIVFEDFARQ--RLKGMKDDQEE-E-EVPNSPHLNNT---RGGLLGDLASSDVAVELPFTLMHP--KPRE---EP-----VR------------------------RE--------------VPENEAPIDTNLIELDTN----------DDDIVFEDFARQ--RLKGMKDDQEE-E-EVPNSPHLNNT---RGGLLGDLASSDVAVELPFTLMHP--KPKE---EP-----AH------------------------RD--------------VPENEAPIDTNLIELDTN----------DDDIVFEDFARQ--RLKGMKDDKEDEE-ERTNSPQLNDR---RGGLLGDLASSDVAVELPFTLMHP--KPKE---EP-----VH------------------------RD--------------IPENEAPIDTNLIELDTN----------DDDIVFEDFARQ--RLKGMKDDKEDEE-ERTNSPQLNDR---RGGLLGDLASSDVAVELPFTLMHP--KPKE---EP-----LH------------------------RD--------------VPENEAPIDTNLIELDTN----------DDDIVFEDFARQ--RLKGMKDDKEEDD-EGTNSPQLNDR---RGG--------DVAVELPFTLMHP--KPKE---EP-----LH------------------------RD--------------VPENEAPVDTNLIELDTN----------DDDIVFEDFARQ--RLKGMKDEKEEDE-EGTNSPQLNDR---RGGLLGDLASSDVAVELPFTLMHP--KPKE---EP-----LH------------------------RD--------------APDNEAPIDTNLIELDTN----------DDDIVFEDFARQ--RLKCMKDEKEEEEVDGTNSPQLNDR---RGGLLGDLASSDVAVELPFTLMHP--KPTE---EP-----LH------------------------RE--------------VPENEAPIDTNLIEFDTN----------DDDIVFEDFARQ--RLKGMKEDKEEEE-EGTNSPLMNDR---RGGLLGDLASSDVAVELPFTLMHP--KPIE---ES-----FY------------------------RD--------------APENEAPIDTNLIEFDTN----------DDDIVFEDFARQ--RLKGAKDDKDEED-EGANSPKLNDR---RGGLLGDLASSDVAVELPFTLMHP--KPIE---ES-----FY------------------------RD--------------VPEHEAPIDTNLIEFDTN----------DDDIVFEDFARQ--RLKGAKDDKDEEE-EGANSPKLNDR---RGGLLGDLASSDVAVELPFTLMHP--KPLE---ES-----IY------------------------RD--------------APENDAPIDTNLIEFDTN----------DDDIIFEDFARQ--RLIGAKDDKDEDE-EGADSPKLNDR---RGGILGDLAASDVSVELPFTLMHP--KPLE---ES-----IY------------------------KD--------------AP-DEAPIDTNLIQFDTN----------DDDIIFEDFARQ--RLTEAKD--DEDE-EPVDSPKLEDR---RGGLLGDLATSDVILELPFTLMHA--KPEE---EA-----FY------------------------RE--------------VPENEAPIDTNLIEFDTN----------DDDIVFEDFARQ--RLKGLKDDNDEDE-DGVNSPQHNDR---RGGILGDLATSDVIVELPFTLMHA--KPEE---EA-----FY------------------------RE--------------VPENEVPIDTNLIEFDSN----------DDDIVFEDFARQ--RLKGLKDDNDDDE-DGVNSPQHNDR---RGGILGDLATSDVIVELPFTLMHA--KPEE---ET-----FY------------------------RE--------------VPENEVPIDTNLIEFDTN----------DDDIVFEDFARQ--RLKGLKDDNDDDE-DGVNSPQHNDR---RGXXLGDLATSDVVVELPFTLMHA--KPEE---ET-----FH------------------------RE--------------ATENEAPIDTNLIEFDTN----------DDDIVFEDFARQ--RLKGMKDENEENE-DGANSPQHNDR---RGGILGDIATSDVAVELPFTLMHP--KPAE---ES-----LS------------------------RAG-----------SVAPDSDVPVDADLIVFETNGDA------WDDDIVFEDFARQ--RLKGAKDE-EEEE-VAPDSPNGK-----XXXXXXXXXXXXXXXXLPFTLMHP--KPAE---ES-----LS------------------------RAG-----------SVAPDSETPVDADLIVFETNGEA------WDDDIVFEDFARQ--RLKGAKDE-EEEE-GAPDSPNGK-----RGG--------DVAVELPFTLMHP--KPVE---ES-----LP------------------------STP-----------CALPESDAPLDTNLIEFDTNGDG------HEDDIVFEDFARQ--RLKGIKDE-NDE-----DSPLC------RGG--------DVAVELPFTLMHP--KPVE---ES-----LP------------------------STP-----------CASPE------------NINGQG------HEDDIVFEDFARQ--RLKGIKDE-NDE-----DSPLC------RGG-----------------------------------------------------------------------------------------------------------------------------------------------------RGG--------DVAVELPFTLMHP--KPVE---ES-----LP------------------------STP-----------CALPESDAPLDTNLIEFDTNGEG------HEDDIVFEDFARQ--RLKGIKDE-NDE-----DSPLC------RGG--------DVSVELPFVLMHP--KPHD---HI-----PLP-----------------------RPQ-----------SAAPETDVPVDTNLIEFDTNYA-------TDDDIVFEDFARL--RLKGMKDD-DYDD--------QLC----RGG--------DVSVELPFVLMHP--KPHD---HI-----ALP-----------------------RPQSAPTHPPTLLPSAVPETDAPVDTNLIEFETNYA-------TDDDIVFEDFARL--RLKGLKDE-DYDD--------QFC----RGG--------DVSVELPFVLMHP--KPHD---HS-----THS-----------------------KPQ-----------SAAPEINDPVDTNLIEFETNYG-------TDDDIVFEDFARL--RLKGMKDE-DYDN--------QSC----RGG--------DVSVELPFVLMHP--KPHD---HP-----THS-----------------------KPQ-----------SAAPEINDPVDTNLIEFETNYG-------TDDDIVFEDFARL--RLKGMKDE-DYDN--------QFC----RGG--------DVSVELPFVLMHP--KPPE---QF-----ALT-----------------------RPQ-----------SVVPEADTPVDTNLIEFETNYG-------QDDDIVFEDFARL--RLKGVKDD-KDDD--------HF-----RGG--------DVAVELPFVLMHP--KPPE---QP-----SPT-----------------------QPP-----------AAVPESDTPVYANLIEFETNYG-------QDEDMVFEDFARL--RLKGLKDD-EDTD--------HFC----RGG--------DVSVELPFVLMHP--KPPE---QA-----PPL-----------------------QPP-----------TAVQETDAPIDTNLIEFETNYG-------QDDDIVFEDFARL--RLKGLKDDKEDDD--------HFC----RGG--------DVAVELPFVLMHP--KPPE----------NIS-----------------------RPL-----------SEYPQTDAPVDTNLIEFDTNFA-------QDDDIVFEDFARL--RLKGLKDDKDDDE--------AYC----RGG--------DVTVELPFVLMHP--KPPE----------PAP-----------------------RPI-----------SEFPQTDVPVDTNLIEFETNYL-------QDDDIVFEDFARL--RLKGLKDDQDDDE--------AYC----RGG--------DVSVELPFVLMHP--KPSD---QP-----SS------------------------RPQ-----------SAIPETDAPVDTNLIEFETK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGG--------DVSVELPFVLMHP--KPSE---SS-----LS------------------------HST-----------SAVPMLDPPIDTNLIEFDTNS-LI-----PDDDIVFEDFARL--RLKGVIDKEED----------C------RGG--------DVSVELPFVLMHP--KPSE---QP-----NS------------------------RPQ-----------SAVPETDVPVDANLIEFETNN-FS-----QDDDFVFEDFARL--RLKGMKDEEDDHF--------C------RGG--------DVSVELPFILMHP--KPVE---PP-----LS------------------------RPQ-----------SAVPEMDPPIDTNLIEFETN--VS-----QDDDFVFEDFARL--RLKGAVDDKDED---------C------RGG--------DVSVELPFVLMHP--KPVE---Q------PVS-----------------------RPQ-----------SVLPESEVPVDTNLIEFETNFG-------QDDDIVFEDFARL--RLKGLKDEKDDDD--------HFC----RGG--------DVSVELPFVLMHP--KPVE---Q------PMS-----------------------RPQ-----------SVLPESEVPVDMNLIEFEANFS-------HDDDIVFEDFARL--RLKGQKDEKDEDD--------HFC----RGG--------DVSVELPFVLMHP--KPIE---Q------PVS-----------------------RPQ-----------SVQPEIEPPVDTNLIEFETNFN-------QDDDIVFEDFARL--RLKGLKDDKDDDD--------QFC----RGG--------DVSVELLFVLMHP--KPIE---Q------PIS-----------------------RPQ-----------SVLPEMEPPVDTNLIEFETNFS-------QDDDIVFEDFARL--RLKGLKDEKDDDD--------HFC----LGG--------EVCVELPVTLMHS--KPAD---VD-----PII--GSPAKV---------------ASG-----------KPA---PEPIDHNLISFDTNA-SP-----PNDDIVFEDFARM--RLQGEAATGGDGQ--------TA-----LGG--------EVCVELPVTLMHS--KPAD---VD-----PII--GSPVKT---------------IAD-----------KPSNSTAQPIDHNLISFDTNASSP-----PTDDIVFEDFARM--RLQGEAAAGGDGH--------A------LGG--------ECVLELPVSLMHA--MPEP---EP-----EKL--EEKPKS---------------EEQ-----------KQASNANPDLEPNLISFDTKG-TP-----AGDDIVFEDFARM--RLQVTPQGECEKQ--------HEQK--------------DLSVELPFTLSHP--KPET---PP-----P------SPKP---------------DRA-----------Q--------------GFD------------------------------------------------------YGG--------DLSVELPFTLSHP--KPET---PP-----P------SPKP---------------DRA-----------Q-----EAPVDTNLINLETSE-TPDSYGKDDDDIIFEDFARM--RLKDHEGGDTE----------A------YGG--------DLAVELPFTLTHP--KPPE---SP-----P------PSRP---------------EST-----------QPGNEADIPVDTNLISFDTNG-EANQFPDRDDDFIFEEFARL--RVKGAEGDEAE----------A-----LLNG--------DLVAELPFTLMHP--KPEE---EE-----HPLLGERSPRASLAGGGLPLVSMSDGETE-----------SATGGQDVPTTTNLIQLDDDEA-------QDDDIIFEDFARL--RLKGAETEA------------------.......490.......500.......510.......520.......530.......540.......550.......560.......570.......580.......590.......600.......610.......620.......630...

405 404 402 404 418 441 409 375 403 361 395 396 391 392 392 399 387 383 384 384 390 390 365 422 422 422 420 427 419 385 405 391 392 393 388 392 390 398 395 395 393 392 394 394 387 389 388 386 357 362 365 394 394 394 394 394 393 418 418 429 430 453 418 440 414 412 461 418 418 418 395 364 354 432 393 423 422 243 332 175 410 409 420 409 418 411 484 429 408 408 378 327 406 408 406 409 409 310 310 418 418 419 367 441 439 470

RGS9_Human_NP-001159405 RGS9_Cattle_NP-776595 RGS9_Koala_XP-020856566 RGS9_Opossum_XP-007482817 RGS9_Pigeon_XP-013225676 RGS9_Quail_XP-015735540 RGS9_Ground-tit_XP-014111246 RGS9_Turtle_XP-014424786 RGS9_Python_XP-007421674 RGS9_Garter_snake_XP-013926935 RGS9_X.laevis_XP-018090999 RGS9_Florida_gar_21856-1-2 RGS9_Bowfin_26690-1-1 RGS9_Zebrafish_NP-001314729 RGS9_Medaka_XP-004071947 RGS9_Reef_shark_51239-1-1 RGS9_Bamboo_shark_45486-8-1 RGS9_Bluespot_ray_37076-2-1 RGS9_Western_ray_41046-8-8 RGS9_Elephant_shark_XP-007886905 RGS9_M.mordax_34174-1-2 RGS9_G.australis_56905-1-2 RGS9_E.cirrhatus_137654-1-3 RGS11_Human_NP-899180 RGS11_Cattle_NP-001069282 RGS11_Koala_XP-020862219 RGS11_Opossum_XP-001372371 RGS11_Chicken_XP-001231808 RGS11_Pigeon_XP-005508486 RGS11_Ground-tit_XP-014107999 RGS11_Turtle_XP-007066592 RGS11_Alligator_XP-014457643 RGS11_Anole_XP-003227697 RGS11_Nanorana_XP-018423234 RGS11_Xenopus_NP-001096497 RGS11_Florida_gar_29521-1-2 RGS11_Bowfin_26311-1-2w1-5 RGS11_Zebrafish_XP-005170921 RGS11_Medaka_XP-011484761 RGS11_Reef_shark_50711-1-1 RGS11_Bamboo_shark_41815-1-3 RGS11_Bluespot_ray_32308-1-2 RGS11_Western_ray_41178-3-3 RGS11_M.mordax_39562-1-2 RGS11_G.australis_47497-1-2 RGS11-Partial-Trimmed_P.marinus_S4RW55 RGS11-Partial-Trimmed_L._camtschaticum_JL5627 RGS11_E.cirrhatus_136920-1-2 RGS_S.kowalevskii_XP-006813443 RGS-Partial_S.purpuratus_XP-011673106 RGS6_Human_NP-001191345 RGS7_Human_NP-002915

MT--------IRH----Q----GQQYRPRMAFLQKIEALVKDMQNPET-GVRMQNQRVLVTSVPHAMTGSDVLQWIVQRLWISSL-EAQNLGNFIVRYGYIYPLQDPKNLILKPD-GSLY MT--------IRH----Q----GQQYRPRMAFLRKIEALVKDMQDPDT-GVRVQNQKVKVVSIPHAMTGSDVLQWISQRLWISGL-EAQNLGNFIVKYGYIYPLQDPRNLTLKPD-SSLY MT--------IKH----Q----GQQYRPRMAFLRKIEDLVKEMQNPET-GIRMQSQRVMITNIPHAMTGNDILQWIAQRLWIGNI-EAQNLGNFIVKYGYIYPLQDPKNLILKPESNSLY MT--------IRH----Q----GQQYRPRMAFLRKIEALVKEMQNPET-GIRMQSQRVMITNIPHAMTGNDILQWIAQRLWIGHM-EAQNLGNFVVKYGYVYPLQDPKNLNLKPDNSSLY -----------------------------MAFLKKIEALMMEMQNPDT-GVKTQTQRVMITNIPHAVAGNDVLQWILQRLQITQE-EALHLGDLFVKYGYIYPLQEPKNLTLKTD-GSLY MT--------VARL--DQ----GQRYRPRMAFLKKIEALIMEMQNPDT-GIKTQTQRVMITNIPHAVAGNDIFQWILQRLQITNE-EALHLGDLFVKYGYIYPLQEPKNLTLRTD-GILY MT--------VTRL--DQ----GQRYRPRMAFLKKIETLMMEMQNPDT-GIKTQTQTVMITNIPHAVTGVDIMQWILQRLQITAE-EALHLGDLFVKYGYIYPLQEPKNLTLKAD-GSLY ----------------------------------------MEMQNPDT-GIKMHTQRVMITNIPHAMTGSDILQWIMQHLKIVEE-EALNLGNLLVKYGYIYPLQEPKNLILKTD-SSLY MT--------ITHI--DQ----GQRYRPRMAFLKKLEILLVEMQDPHS-GIKIQTQKIINTSIPHAMTGSDILKWIMQRLQITSE-EALNLGSMIIKCGYIYPLQERKNLTLRAD-SSLY MT--------IAQI--DQ----GQRYRPRMAFLKKLEVLLVEMQDPQS-GIKTQIQNMPENSIPHTVVGCDMLQWIVKRLEITNE-EGLHLGSMMVKCGYVYPLQDRKNLNLKGD-TSLY MT--------VKHL--DQ----GQHFRPRMAFLKKIEALLLEMQDADT-GIKTHTQRLMITTIPHAVTGHDILEWLIQRLQIADE-EAQHLGNLMVRCGYIYPLQEPTNLVLNVD-SSLY MT--------INKLC-DY----SQHYRPRMACLKKIESLVVEMQDPNN-GIKTQTQRLVITTIPHAITGLDLLQWLANRLQVETE-EAQNIGNLVVQYGYIYPLQDHKNLILKPD-GSLY MT--------INKLC-DH----SQKYRPRMACLKKIESLVVEMQDPNS-GIKTQTQRLVITTIPHAITGLDILQWIINRLQLENE-EAQNTCNLLVQYGYIYPLQEHKNLILKPD-ASLY MT--------IRSDR-DH----GQHYRPRMTCLKKLEAMVVQMQDPST-GVKGSDQKLNVTTIPHVMTDQDIIAWIAKHMSMENN-EAQAFGTMLVAYGYIYPLQDHKKLVLKSD-GSLY MT--------IRTVRPDR----GQHFHPRMHCLKKLEATVVEMQDPKT-GVKGSEQKLNVTTIPHVIAGNDIIAWITNKMKVTQE-EAGVFGTMLVAYGYVYPLQNHKKLVMCND-GSLY MT--------IKSF--NQ----GQHYRPRMAFLRKIEPLMMEMQDPNT-GIKTQAQRLVITTIPHAMTGHDISEWLIQRLQITDE-EGYVLGNMIVKLGYIYPLQEPKNLVLRQD-SSLY MT--------IRNF--NQ----GQQYRPRMAFLRKIEPLIKEMQDPNT-GIKTQTQRLIITTIPHAMTGQDVLEWLIQRLQITDE-EGYILGNMIVKLGYIYPLQEPRNLVLKHD-NSLY MT--------VKSF--NQ----GQQFRPRMAFLRKIEPLIMEMQDPGT-GIKTQTQRLMITTIPHAMTGNDVLEWLIHRLQVTDE-EGSLLGNLLVKLGYIYPLQEPKNLVLKPD-NSLY MT--------VKSF--NQ----GQQFRPRMAFLRKIEPLIMEMQNPGT-GIKTQTQRLMITTIPHAMTGNDVLEWLIQRLQVTEE-EGSLLGNLLVKLGYIYPLQEPRNLVLRPD-NSLY MT--------IKSL--SQ----GQHHRPRMAFLKKIESLVIEMQDPEN-GIKTQTQRVMIATIPHALTGQDLLEWLIQRLQIEDE-ECQNLGNLIVNLGYLYPLQEPKNLILRPD-SSLY MK--------IHRL--EQ----NQRHRPRQHHLAKLGALVVTMQDSEH-GVPMRDQLLGDAVIPDSTTGRDLVQWLEQRLQVTAD-EAQQLGWLLVLCGFIYPLCEPRNLVLRAD-DSFY MK--------INKL--DQ----SQQHRPRQQHLTKLGALVVSMQDPER-GVPMRDQLLSDTIIPDATTGHDLMRWIEQRLQITAD-EALQLCWLLVACGFVYPLSEPRNLAMRPD-GSLY MK--------INKL--DQ----GQHYRPRMAFIKKLQSLVGAMQKRED-GVIFRNQRLITTTIPHAIMGFDLLEWFIESLQITYE-EAIHLGTLVICNGFIYPVSEPRDLTLRND-SSLY MA--------AGP----AP--PPGRPRAQMPHLRKMERVVVSMQDPDQ-GVKMRSQRLLVTVIPHAVTGSDVVQWLAQKFCVSEE-EALHLGAVLVQHGYIYPLRDPRSLMLRPD-ETPY MA--------ASP----AP--LGGHPRVQRPHLSKMERMVVTMQDPDQ-GVRMRSQRLLVTVIPHAVNGSDIVEWLMQKYSIAED-EALHLGSLLVQHGYLYPLRDPRRLELRPD-ETPY MC--------VHKGFRWSLAWSFSRSNGSLLKSFQMERVIVGMQDPDM-GVKMRNQRLLITVIPHAMTGSDIVEWLIQKFSISEE-ESLHLGNLIVKHGYIYPLKDPRCLVLRAD-DSPY MT--------INS----LH--QGGSYKVQMPCLSKMERVIVGMQDPDM-GVKMRNQRLLITVIPHAMTGHDIVEWLIQKYSISEE-ESLHLGNLIVKHGYIYPLKDPRCLVLRAD-DSPY MT--------IGG----PR--GGGQPRLQMPCLGKMERMIVSMQDPDM-GIKMRNQRLLITVIPHAMTGNDIVEWLIQKYGISEE-ESLHLGNLIVRHGYIYPLRDPRSLVLRAD-ESPY -----------------------------------MERMIVSMQDPDM-GIKMRNQRLLITVIPHAMTGNDIVEWLIQKYGISEE-ESLHLGNLIVKHGYIYPLRDPRSLVLRAD-ESPY -----------------------------------MERMIVSMQDPDM-GIKMRNQRLLITVIPHAMTGNDIVEWLIQKYGISEE-ESLHLGNLIVKHGYIYPLKDPRSLVLRAD-ESPY -----------------------------------MEHVIVSMQDPDM-GIKMRNQRLLITVIPHAMTGNDIVEWLIQKYAISEE-ESLHLGNLLVKHGYIYPLKDPRSLVLRAD-DSPY -----------------------------------MERMIVIMQDPDM-GVKMRNQRLLITVIPHAMTGNDIVEWLIQKYTISEE-ESLHLGNLIVKHGYIYPLKDPRNLVLRAD-ESPY MT--------ISA----LRPGAGGNSRLQLPCLAKMERVVVGMQDPDM-GVKVRSQRLLITVIPHALTGSDIVQWLVQKYAISEE-EALHLGNLIVRHGYIYPLKDPRSLALRPD-ETPY -----------------------------------MERLIVAMQDPDI-GIKMRNQRLLITVIPHAMTGNDVVEWLIQKLSINEE-ESLHLGNLLVKYGYIYPLKDPRNLILRAD-DSPY MT--------INS----LQ--PGKNCKVQMSCLAKMERLVVSMQDPDM-GIKMRNQRLLITVIPHAMTGNDVVEWLIQKLSISEE-EAQHLGNLLVRYGYIYPLKDPRNLILRAD-DSPY MT--------INT------LSQGGNYKVQMPCFTKMERVIVAMQDPDM-GIKMRNQRLLITVIPHAVTGNDIVEWLIQKFSITEE-EALHVGNLIVKFGYIYPLKDPKTLILRPD-DSPY MT--------INT------LKQGGSYKVQMPCLTRMEHIIVAMQDPDM-GIKMRNQRLLITVIPHAMTGNDIVEWLIQKFSITEE-EALHVGNLIVKYGYIYPLKDPKTLILRPD-ESPY MT--------INT------LSQDGVQRQQMPCLTKMERMIISMQDPDM-GIKLRNQRLLITVIPHAMTGADIVEWLIKKLTISDE-EAAHIGQFLVKYGYIYPLKEPRNLILKAD-ESAY MT--------INT------MAKGGWQREQMICFRMMEKMIVAMQDPDM-GMKLRNQRLLITVIPHAMTGHDIIIWLIHKYSVCEE-EALHLGGMLVKYGYIYPLKEPRSLVLQPD-ESPY ------------S-------ARIHTATIQMACLNKMEKVILAMQDPDI-GIKMKYQKLLITAIPHAVTGSDVVEWLLQNLSISEE-ESLHLGNLIVKYGYIYPLKDSKNLILRPD-DSPY MT--------IHT-------SSTHTTKIQMACLAKMEKVIVAMQDPDV-GIKMKYQKLIITGIPHAVTGNDIVEWLIQNFSISEE-ESLHLGDLIVKYGYIYPLKDPKNFVLRAD-DSPY MT--------IHG-------TGSQLGGAQMACLAKMETLIVAMQDPDT-GIKMRIQRFLV-PIPHLMTGNDIFQWLLQNVSATEE-ESFHLGDLIIKYGYIYPLKDPRSLVLRVD-DSPY MT--------IHN-------TRSHIGSAQMACLAKMEKLIVAMQDPDT-GIKMRIQRFLVTPIPHAMTGNDIFEWLLQNLSTSEE-ESFHLGDLIIKYGYIYPLKDPKNLVLRVD-DSPY -----------------------------MTSSKKVGCLVRRMQDTHT-GVPLLTRHWRTIAVPNAGIGHNITDWILRNLKITLE-ETFNLCNLLVRYGYLYPVYPATHLGFHL--NKLY -----------------------------MASSEKVGCLICRMQDTHT-GVPLLTRHFLTFAIPNASIGHNISDWILRDLKTTQE-ETSNLCNLLVRYGYLYPVHPATQLGFHP--SKLY -----------------------------------VECLVCRMQDKQT-GVPLLTRHLITFAIPNASIGYNIADWMLRDMKTTQE-ETYNLCNLLVKYGYLYPVHPAAHLGFHP--SKLY ------------------------------------------MQDKQT-GVPLLTRHFITFAIPNASIGYNIADWMLRDMKITQE-ETSNLCNLLVKYGYLYPVRPAARLGFHP--SKLY -----------------------------MSGFEKVEMLVGAMQDPEM-GVNMFDQRLLLTVIPHAVRGHEILTWIIRNLKTSDE-EALNLSNLLVRTGYVYPLLEPANFSLQPD-DSIY MVGMSEVGGDSGMV--GECGVGGENEMVGVSEVGGENKIVGVSEVGGE-SRISLIQLYVNDSIFVFITGYDLIEWLMENLDIQDSIEALHLANLFCQYGYIFPVQDIKNLVVKDD-STLY MT---------------------------------ASQVV-------------------------------------------------------------------------------MAQGSG-DQRAVGV--AD----PEESSPNMIVYCKIEDIITKMQDDKTGGVPIRTVKSFLSKIPSVVTGTDIVQWLMKNLSIEDPVEAIHLGSLIAAQGYIFPISD-HVLTMKDD-GTFY MAQGNNYGQTSNGV--AD-------ESPNMLVYRKMEDVIARMQDEKN-GIPIRTVKSFLSKIPSVFSGSDIVQWLIKNLTIEDPVEALHLGTLMAAHGYFFPISD-HVLTLKDD-GTFY 1.......10........20........30........40........50........60........70........80........90.......100.......110.......120

101 101 102 102 88 103 103 77 103 103 103 104 104 104 105 103 103 103 103 103 103 103 103 103 103 109 103 103 82 82 82 82 105 82 103 103 103 103 103 98 102 101 102 87 87 81 74 88 116 7 111 108

RGS9_Human_NP-001159405 RGS9_Cattle_NP-776595 RGS9_Koala_XP-020856566 RGS9_Opossum_XP-007482817 RGS9_Pigeon_XP-013225676 RGS9_Quail_XP-015735540 RGS9_Ground-tit_XP-014111246 RGS9_Turtle_XP-014424786 RGS9_Python_XP-007421674 RGS9_Garter_snake_XP-013926935 RGS9_X.laevis_XP-018090999 RGS9_Florida_gar_21856-1-2 RGS9_Bowfin_26690-1-1 RGS9_Zebrafish_NP-001314729 RGS9_Medaka_XP-004071947 RGS9_Reef_shark_51239-1-1 RGS9_Bamboo_shark_45486-8-1 RGS9_Bluespot_ray_37076-2-1 RGS9_Western_ray_41046-8-8 RGS9_Elephant_shark_XP-007886905 RGS9_M.mordax_34174-1-2 RGS9_G.australis_56905-1-2 RGS9_E.cirrhatus_137654-1-3 RGS11_Human_NP-899180 RGS11_Cattle_NP-001069282 RGS11_Koala_XP-020862219 RGS11_Opossum_XP-001372371 RGS11_Chicken_XP-001231808 RGS11_Pigeon_XP-005508486 RGS11_Ground-tit_XP-014107999 RGS11_Turtle_XP-007066592 RGS11_Alligator_XP-014457643 RGS11_Anole_XP-003227697 RGS11_Nanorana_XP-018423234 RGS11_Xenopus_NP-001096497 RGS11_Florida_gar_29521-1-2 RGS11_Bowfin_26311-1-2w1-5 RGS11_Zebrafish_XP-005170921 RGS11_Medaka_XP-011484761 RGS11_Reef_shark_50711-1-1 RGS11_Bamboo_shark_41815-1-3 RGS11_Bluespot_ray_32308-1-2 RGS11_Western_ray_41178-3-3 RGS11_M.mordax_39562-1-2 RGS11_G.australis_47497-1-2 RGS11-Partial-Trimmed_P.marinus_S4RW55 RGS11-Partial-Trimmed_L._camtschaticum_JL5627 RGS11_E.cirrhatus_136920-1-2 RGS_S.kowalevskii_XP-006813443 RGS-Partial_S.purpuratus_XP-011673106 RGS6_Human_NP-001191345 RGS7_Human_NP-002915

: : : : : :: . **::.* RFQTPYFWPTQQWPAEDTDYAIYLAKRNIKKKGI--LEEYEKENYNFLNQKMNYKWDFVIMQAKEQY--------------------------RAGKERNKADRYALDCQEKAYWLVHRC RFQTPYFWPTQQWPAEDVDYAIYLAKRNIKKKGI--LEEYEKENYNFLNKKINYKWDFVIMQAREQY--------------------------RAGKERNKVDRCALDCQEKAYWLVHRC RFQTPYFWPTQQWSAEDTDYAIYLAKRNIKKRGI--LEEYEKENYSFLNKKINHKWDFVIMQAKEQY--------------------------KTGKERKKADRYALDCQEKAYWLVHRC RFQTPYFWPTQQWSAEDTDYAIYLAKRNIKKKGI--LEEYEKENYSFLNKKINHKWDFIIMQAKEQY--------------------------KTGKERKKADRYALDCQEKAYWLVHRC RFQTPYFWPVQAWPADDTDYAIYLAKKNIKRKGI--LEEYEKEHYNMLNQKINYKWDFVIMQAKEQY--------------------------KAGKERKKADRYALDCQERAYWLVNRT RFQTPYFWPAQSWPADDTDYAIYLAKKNIKRKGI--LEEYEKEHYNMLNKKINYKWDFVIMQAKEQY--------------------------KAGKERKKADRYALDCQERAYWLVNRT RFQTPYFWPVQSWAADDTDYAIYLAKKNIKRKGI--LEEYEKEHYSMLNQKINYKWDFVIMQAKEQY--------------------------KAGKERKKEDRYALDCQERAYWLVNRT RFQAPYFWPTQQWAADDTDYAIYLAKKNIKRKGM--LEEYEKEHYNLLNKKINYKWDFVVMQAKEQY--------------------------RTGKERKKADRYALDCQEKAYWLVHRT RFQTPYFWPTQQWGAEDTDYAIYLAKKNIKRKGI--LEEYEKEHYNTLNKNINHKWDFIIMQAKEQY--------------------------RTGKERKKEDRYTLECQEKAYWLVQRA RFQTPYFWPTQQWGADDTDYAIYLAKRNIKKKGA--LEDYEKEHYSILNKRINHKWDFIIMQAKEQY--------------------------SAGKERTKEDRYTVDYQEKAYWLVQRA RFQTPYFWPTQQWPAEDTDYAIYLAKKNIRKKGI--LEEYEKENYSNLNKKINHKWDFVVMQAKEQY--------------------------KAGKERKKSDRVVLNCQEKAYWLVHRP RFQTPYFWPAQQWPAEDTDYAIYLAKKNIRKKGM--LEDYEKDNYNMLHKRINHKWDFVVMQAKEQY--------------------------RAGKERKKADRVVFDCQERAYWVVQKP RFQTPYFWPAQQWPAEDTDYAIYLAKKNIRKKGV--LEDYEKENYSVLHKRINHKWDFVVMQAKEQY--------------------------RAGKERKKADRVVFDCQERAYWVVQKP RFQTPYFWPVQKWAAEDIDYAIYLAKRNIRKKGA--LDAHEQEEYDKLHKWMNHKWDFIVMQAKEQY--------------------------HAGKARPKADRVVFDCQERAYWIVHRP RFQTPYFWPKQNWFAEDSDYAIYLTKRNIRKKGM--LEAYEQAHYNHLNEWLNHQWDFIVMQATEQY--------------------------KAGKDRQKPDRVVLDCQERAYWIVNRP RFQTPYFWPTQEWPAEDTDYAIYLAKKNIRKKGI--LEEYEKENYNILNQKINHKWDFIIMQAKEQY--------------------------RAGKERKKADRFVLDCQERAYWLVHRP RFQTPYFWPTQEWPAEDTDYAIYLAKKNICKKGL--LEEYEKENYNNLNMKINHKWDFIIMQAKEQY--------------------------RAGKERKKADRFVLDCQERAYWLVHRP RFQTPYFWPSQEWPAEDTDYAIYLAKKNIRKKGM--LEEYEKENYNLLNKKINHKWDFIIMQAKEQY--------------------------RAGKERKKADRFVLDCQERAYWLVHRP RFQTPYFWPSQEWPAEDTDYAIYLAKKNIRKKGV--LEEYEKENYNLLNKKINHKWDFVVMQAKEQY--------------------------RAGKERKKADRFVLDCQERAYWLVHRP RFQTPYFWPTQQWAAEDTNYAIYLAKRNIRKKGM--LEEYEKENYNLLNKKINHKWDFVIMQAKEQH--------------------------RASKERRKTDRIVLDRQEKAYWQVHRP RFQSPYFWPLYQSENEDTDYAIYLARKNIRHKGA--LEQHEQELYSTLHEKINHEWDYVITQAKEQY--------------------------RSGRERPTCDRVVLENQERAHWLTRRP RFQAPYFWPVYYSQSEDTDYAIYLARKNIRSKGG--LDPYEQELYSTLREKINHEWDYVVCQAKEQY--------------------------RSGRERPKGDRVVLECQERAHWLVRRP RFQSPYFWQCQLWPADDRDYAIYLVKKNIRKKRE--LEGYEKEAYNQLHEKINHQWDFVVMQAKEQI--------------------------RAAKERKKGDRVVMEFQERAYWMVHRP RFQTPYFWTSTLRPAAELDYAIYLAKKNIRKRGT--LVDYEKDCYDRLHKKINHAWDLVLMQAREQL--------------------------RAAKQRSKGDRLVIACQEQTYWLVNRP RFQTPYFWTSTLWPAAELDYAIYLAKKNIRKQGA--LVDHEKEHYQQLHRKINHAWDLVMMQAREQL--------------------------RAAKQRRKGDRLVIACQEQTYWLVNRP RFQTPYFWMSTKWPAAELDYAIYLAKKNIRKQGD--LIEHEKDYYNQLHKKINHTWDFVVMQAREQL--------------------------RAVKQRRKGDRIVIECQEQTYWLVNRP RFQTPYFWMSTKWPAAELDYAIYLAKKNIRKQGD--LIDHEKDYYNQLHKKINHTWDFVVMQAREQL--------------------------RAAKQRRKGDRIVIECQEQTYWLVNRP RFQTPYFWTSTKWPATELDYAIYLAKKNIRKQGE--LIEHEKDNYNLLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKGDRIVIDCQEQAYWLVNRP RFQTPYFWTSTKWPATELDYAIYLAKKNIRKQGD--LIEHEKDNYNLLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKGDRIVIDCQEQAYWLVNRP RFQTPYFWTSTKWPATELDYAIYLAKKNIRKQGD--LIEHEKDNYNLLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKGDRIVIDCQEQAYWLVNRP RFQTPYFWTSSKWPATELDYAIYLAKKNIRKQGD--LIDHEKDNYNLLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKGDRIVIDCQEQAYWLVNRP RFQTPYFWTSTKWPATELDYAIYLAKKNIRKQGD--LIDHEKDNYNLLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKGDRIVIDCQEQAYWLVNRP RFQTPYFWMSTKWPATELDYAIYLAKRNIRKQGE--LIGHEKDDYNQLHKRINHSWDFVVMQAREQL--------------------------RAAKQRRKGDRIVIECQEQAYWLVNRP RFQTPYFWTTNKWPATELDYAIYLAKKNIRKQGD--LVEYEKDNYNQLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKGDRIVIECQEQAYWLVSKP RFQTPYFWTTNKWPATELDYAIYLAKKNIRKQGE--LVEYEKDNYNQLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKGDRIVIECQEQAYWLVSKP RFQTPYFWTTNKWPASELDYAIYLAKKNIRKQGE--LVDYEKDNYNLLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKGDRIVLECQEQAYWLLNKP RFQTPYFWTTNKWPASELDYAIYLAKKNIRKQGE--LIDYEKDNYNLLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKADRIVLECQEQAYWLVNKP RFQTPYYWMSTRWPASELDYAIYLAKKNIRKQGE--LIEYEKECYNALHKRINHTWDFVVMQAREQL--------------------------RASKQRRKADRIVLECQEQAFWLVNRP RFQTPYFWTSTRWPASELDYAIYLAKKNIRKQGE--LMEYEKERYSLLHKKINHTWDFVVMQAREQLRYMSTQTWKYVSSHFTQTIFQSALCFRASKQRRKADRIVLECQEQAYWLINRP RFQTPYFWTSYKWPARELDYAIYLAKKNITRQGD--LFDYEKDSYNLLHKRINHSWDFVVMQAREQL--------------------------RAAKQRRKSDRIVLDCQEQAYWFVNRP RFQTPYFWTSYKWPATELDYAIYLTKKNIKRQGD--LVDYEKDSYNLLHKRINHTWDFVVMQAREQL--------------------------RAAKQRRKSDRVVLDCQEQAYWLVNRP RFQTPYYWTSYKWPATELDYAIYLAKKNIKKQGN--LVDYEKDNYNRLHTRINHTWDFVVMQAREQL--------------------------RAAKQRKKSDRIVLDCQEQAYWLVNRP RFQTPYFWTSHKWPATELDYAIYLAKKNIRKQGD--LADYEKDSYNLLHTRINHTWDFVVMQAREQL--------------------------RAAKQRKKSDRIVLDCQEQAYWLVNRP HFQTPYFWPDRHLEASDMDYAVHLVKKNILSNGQ--LAEYEKMRYHQLFKSQHCKWDFVIIQAQEQI--------------------------RTVQERKKGDRMVLANQEQAYWRVARP HFQTPYFWPDRHRGASDMQYAVHLVKKNVLSNGQ--LKEYEKMRYHQLFRSEHHKWDFVINQAQEQI--------------------------CTIQERKKGDRVVLANQEQAYWRVARP HFQTPYFWPDRHREASDVEYAVHLVKKNVLSNGQ--LAEYEKVQYHQLFRSEHHKWDFVIIQAQEQI--------------------------RTLKERRKGDRAVLASQEQAYWRVARP HFQTPYFWPDRHREASDVEYAVHLVKKNVLTNGR--LAEYEKVQYHQLYRSEHHKWDFVIIQAQEQI--------------------------RTLQERRKGDRAVLVSQEQAYWRVARP RFQGPYFWPSKSWPTEDTDYAIYLAKKNVMKNGD--LADYEKARYQHLHAKINHKWDFIIMQAKEQM--------------------------SAVKERRRGDRLVLACQEQAFWLIQRP RFQSPYFWPSQNWDPDNADYAIYLAKRSMRNKQRHGLDDYETNAFTKLQRMLCHKWEFVYMQAEEQI--------------------------RIAKQRKKSDKIVLDSQERAFWRVHRP --------------------------------------------------SILTQYTFSFHPSPAN---------------------------RLAKHRKKADRIVLDAQERAFWRVHYP RFQAPYFWPSNCWEPENTDYAIYLCKRTMQNKARLELADYEAENLARLQRAFARKWEFIFMQAEAQV--------------------------KIDRKKDKTERKILDSQERAFWDVHRP RFQTPYFWPSNCWEPENTDYAVYLCKRTMQNKARLELADYEAESLARLQRAFARKWEFIFMQAEAQA--------------------------KVDKKRDKIERKILDSQERAFWDVHRP .......130.......140.......150.......160.......170.......180.......190.......200.......210.......220.......230.......240

193 193 194 194 180 195 195 169 195 195 195 196 196 196 197 195 195 195 195 195 195 195 195 195 195 201 195 195 174 174 174 174 197 174 195 195 195 195 221 190 194 193 194 179 179 173 166 180 210 50 205 202

RGS9_Human_NP-001159405 RGS9_Cattle_NP-776595 RGS9_Koala_XP-020856566 RGS9_Opossum_XP-007482817 RGS9_Pigeon_XP-013225676 RGS9_Quail_XP-015735540 RGS9_Ground-tit_XP-014111246 RGS9_Turtle_XP-014424786 RGS9_Python_XP-007421674 RGS9_Garter_snake_XP-013926935 RGS9_X.laevis_XP-018090999 RGS9_Florida_gar_21856-1-2 RGS9_Bowfin_26690-1-1 RGS9_Zebrafish_NP-001314729 RGS9_Medaka_XP-004071947 RGS9_Reef_shark_51239-1-1 RGS9_Bamboo_shark_45486-8-1 RGS9_Bluespot_ray_37076-2-1 RGS9_Western_ray_41046-8-8 RGS9_Elephant_shark_XP-007886905 RGS9_M.mordax_34174-1-2 RGS9_G.australis_56905-1-2 RGS9_E.cirrhatus_137654-1-3 RGS11_Human_NP-899180 RGS11_Cattle_NP-001069282 RGS11_Koala_XP-020862219 RGS11_Opossum_XP-001372371 RGS11_Chicken_XP-001231808 RGS11_Pigeon_XP-005508486 RGS11_Ground-tit_XP-014107999 RGS11_Turtle_XP-007066592 RGS11_Alligator_XP-014457643 RGS11_Anole_XP-003227697 RGS11_Nanorana_XP-018423234 RGS11_Xenopus_NP-001096497 RGS11_Florida_gar_29521-1-2 RGS11_Bowfin_26311-1-2w1-5 RGS11_Zebrafish_XP-005170921 RGS11_Medaka_XP-011484761 RGS11_Reef_shark_50711-1-1 RGS11_Bamboo_shark_41815-1-3 RGS11_Bluespot_ray_32308-1-2 RGS11_Western_ray_41178-3-3 RGS11_M.mordax_39562-1-2 RGS11_G.australis_47497-1-2 RGS11-Partial-Trimmed_P.marinus_S4RW55 RGS11-Partial-Trimmed_L._camtschaticum_JL5627 RGS11_E.cirrhatus_136920-1-2 RGS_S.kowalevskii_XP-006813443 RGS-Partial_S.purpuratus_XP-011673106 RGS6_Human_NP-001191345 RGS7_Human_NP-002915

: * : : *..: * *** : * : PPGMDNVLDYGLDRV---TNPNE---V------------------------K---KQTVVAVKKEIMYYQQALMRSTVKSSVSLGGIVKYSEQFSSNDAIMSGCLPSNPWITDDTQFWDL PPGANNVLDYGLDRV---TNPNE---D------------------------Q---KQTVVSVRKEIMYYRQALMRSTVKSSVSLGGIVKYSEQFSSNDAIMSGCLPSNPWITDDTQFWDL PPGTDNVLDYGLNRV---TNPNE---D------------------------K---KITIALVRKENMYYQQALMRSTVKSSVSLGGIVKYSEQFSSNDPIMSGCLPSNPWITDDTQFWEL PPGTDNILDYGLNRV---TNPNE---V------------------------K---KDTISLIRKEIMYYQQALMRSTVKSSVSLGGIVKYSEQFSSNDPIMSGCLPSNPWITDDTQFWEL PPGMLDALEYGLDRV---TDPNE---N------------------------K---KKTMDVYRREIMYYQQAIMKSRVKSSVSLGGLVKYSEQFLSNDPILSGCLPSNPWITDDPEFWDL PPGMLDVLEYGIDRV---TDPNEDKVN------------------------Q---KKTMDVYRREIMYYQQAIMKSRVKSSVSLGGLVKYSEQFLSNDPILSGCLPSNPWITDDPDFWDL PPGMLDVLEYGLDRV---TDPNE---N------------------------K---KNTMEFYRREIMYYQQAIMKTRVKSSVSLGGVVKYSEQFLSNDPILSGCLPSNPWISDDPEFWDL PPGMQDVLEYGLDRV---TDPNE---N------------------------K---KKTIDVYRREIMYYQQAIMKSTVKSSVSLGGIVKYSEQFLSNDPIMSGCLPSNPWITDDAEFWEL PPGMQDALEYGLNRV---TDPCE---N------------------------K---KKTIDVYRREIMYYQQAMMKTTVKSSVSLGGIVKYCEQFCSNDPIISGCLPSNPWLTDDVDFWEL PPGMADTLDYGLDRA---IEPCE---N------------------------K---KKTIEVYRTEIMYLQQSLMKTATKSSVSLGGIVKYCEQFCSNDPIISGCLPSNPWVSDDVDFWEL PPGTLDVLEYGLNRA---TDPNV---V------------------------K---KNTVDHYRRHIMYYQQSLMRSTVKSSVSVGGLLKFCEQYYSHDPIMSGCLPSNPWITDDAMFWEL PTGTVNALEYGLNRF---TDPNA---N------------------------P---KKTYDFYRREIIFSQQSLMKSRVKSSVSIGGLVKYCEQYKNHDPFMAGCLPSNPWITDDTMFWEL PPGTINALDYGLNRF---CDPNV---D------------------------P---KKTYDFYRREIIFNQQSLMRSRVKSSVSIGGIVKYTEQYKNHDPFMAGCLPSNPWITDDTMFWEL PPQTHSAMDYGLDRH---IDPNT---E------------------------E---KKTFDYYRRINIYTQQAIMRSRVKSSVSLGAMLKYITNFNNHDPFLVPCLPSNPWLTDNDAYWEL PRRTHSALDSSPERL---INPKA---E------------------------Q---KITFDQYRRMNIFYQQAIMRSKVKSSVSLGALVKFITTYKNHDPFLAPCLPSNPWQTDHDAYWTL APGSLDVLDYGINRA---TDPNA---D------------------------K---KKTYDYYRREILYYQQLLVRSRVKSSVSLGGLVKYCEQYFTHDPIMSGCLPSNPWLTDDTMFWEL APGTLDVLDYGINRA---TDPNA---D------------------------K---KKTYDYYRREILYYQQALVRSRVKSSVSLGGMMKYCEQYFTHDPIMSGCLPSNPWLTDDTTFWEL EPSSLDALDYGINRA---TDPNV---N------------------------K---RKTYDSYRREIMYYQQALVRSRVKSSVSLGGILKYCEQYFTHDPIMSGCLPSNPWITDDTMIWEL EPGSLDVLDYGINRA---TDPNM---N------------------------K---RKTYDSYRREIIYYQQALVRSRVKSSVSLGGIVKYCEQYFTHDPIMSGCLPSNPWITDDTTLWEL PPGALDVLDYGINRA---TDPNE---N------------------------KVEQKMTYDYYRREIMYYQQALVRSRVKSSVSLAGIVKYSEQYFTHDPIMSGCLPSNPWLTDDVMFWEL PPGAFHALDCGLNRV---VKPGT---E------------------------E---KPSIEFYRRQVMYYQQAVSRSRTKSTISLGSLVKYAEAFRVYDPLLAPCLPSNPWVTDDTMLWTL PPGAFHALESGLNRV---VEPGT---E------------------------E---KPSVEHYRRQVMFYQQAASRSRSKSSVTLGSMVKYTESFSVYDPLLATCLPSNPWVTDDTMCWTL PLGMVNSLEYGVDKV---VDPNS---P------------------------Q---DDTVGCVRQQIMYYHHSLNRSGSKSSLSLEKLLKQCNQYLSHDPILTPCLPQNPWISDDTFYWVL PPGAPDVLEQGPGRGSCAASRVL---M----------------------------TKSADFHKREIEYFRKALGRTRVKSSVCLEAYLSFCGQRGPHDPLVSGCLPSNPWISDNDAYWVM PPGAPSVLEQGPGRGPCTAGRVP---M----------------------------TKTLDFYRREVECLRRALGRARVKSSTCLEAYLKFSSQHGPHDPIMSGCLPSNPWVTDDDAYWAM PPGVLNVMEQGPERGNCYTSRVQ---M----------------------------TKNMDFYKREIEYCKKAMGRTRVKSSICLEAYLKFNDQYMPHDPIMSGCLPSNPWITDDTDYWSM PPGALNVMEQGPERGNCYTSRVQ---M----------------------------TKNMDFYKREIEYCKKAMSRTRVKSSICLEAYLKFNDQYMPHDPIMSGCLPSNPWITDDTDYWSM PPGAPNVLEQGPERRNCHTTRVQ---L----------------------------AKNMDYYKREIEYCRKAMARTRVKSSICLEGYLKFNEQYVPHDPIMSGCLPSNPWITDDTTYWAM PPGAPNVLEQGPERRNCQTTRVQ---L----------------------------TKNMDYYKREIEYCRKAMARTRVKSSICLEGYIKFNEQYVPHDPIMSGCLPSNPWITDDTTYWAM PPGAPNVLEQGPERRNCHTTRVQ---L----------------------------TKNMDYYKREIEYCRKAMARTRVKSSICLEGYIKFNEQYVPHDPIMSGCLPSNPWITDDTTYWAM PP------------------------------------------------------ENTDYYKREIECCRKAMGRTRVKSSICLEGYIKFNEQYVPHDPIMSGCLPSNPWITDDTTYWAM P------------------------------------------------------TKNMDYYKREIEYCRKAMGRTRVKSSICLEGYVKFNEQYVPHDPIMSGCLPSNPWITDDNTYWTM PPGVSNVLEQGPERRNCHSTRVQ---L----------------------------EKNTDFYKREIRYFRAAMVRTRVKSSVCLEGYIRFNEQYVPHDPVMSGCLPSNPWITGDTMYWTM SPGCLNVMEYGPEKGDCYTGRMQ---L----------------------------GKNADYYKREMEYLRKAMARTRVKSSICLEGFIKFSEQYLPHDPIMSGCLPSNPWITDDVTYWTL SPGCLNVMEHGPERGNACGGTMQ---L----------------------------GKNIDFYKREMDYLKKAMGRTRVKSSICLEGFLKFSEQYLPHDPIMSGCLPSNPWITDDVTYWTI PPGALDVLERGPERRNCLNSRVQ---------------------------------LNSEYYKKEIEHSRKALSRTRVKSSICLEGFVKYSELYHCHDPIMQGCLPSNPWITDDTMHWSM PPGAFDILEHGPERRNCLNSRVQ---------------------------------LNSEYYKKEIEHTKKALGRTRIKSSICLEGFVKFSEQYLSHDPIMQGCLPSNPWITDDAMHWSM PPGTLDIMNYGPERRT-MNTRVQ---------------------------------LNAEFYKKEIEHNRMSLARNRVKSSICLESYVKYCEQYQPHDPIMQGCLPSNPWITDDVLFWNM PPGVHNMLDVGLERQNNFNLRAT---------------------------------LNSDFYKNEIEVYRRALGRNRVKSSICLEGFLKYSEQYQNHDPIMQGCLPSNPWISDDTAHWTM PPGAVDIMESAPQKNNIHTTKVK---------------------------------LNSEFYKREIEYYKKALTRSRVKSSLSLEGFVKHCEQYLPHDPIVSGCLPGNPWITSGTLFWTM PPGAINIMESTPKGSNIHTTKVT---------------------------------LNSAFYKREIEYYKKALTRSRVKSSVSLEGFVKHCEQYLLHDPIMSSCLPSNPWITSGTLYWTM PPGALNIMESGPRRNTIQARIKL---------------------------------KNSAYYKKEIEYYTNALTRSRVKSSISLEGFVRHCEQYLLHDPIMSGCLPSNPWITSGTIYWTM PPGALNIMESGPRRNNIQARIKL---------------------------------KNSAYYRKEIEYYKNALTRSRVKSSISLEGFVKHCEQNLLHDPIMSGCLPSNPWITNGTMYWTM PPGLDSDALHGPVKHT-MALHRD---------------------------------QVVELYKKEILYCRKALGRARLKPSVSLEGLLKYSEQFSPLDPLLSGCLPNNPWMTDDTTYWVL PPEFDSDALHGPVKHT-IALHRD---------------------------------QVVELCKKEIVYCRKALGRARLKPSVSLEGLLKYSEQFSPLDPLLSGCLPGNPWITDDTTYWVL PPGLDNDALNGPVKHT-MALHRD---------------------------------Q--------IVYGRKALGRARLKPSVSLEGLIKYSEQFTPLDPLLSGCLPNNPWMSNATTYWVL PPGLDNDALNGPVKHT-MALHRE---------------------------------QVAELYKNQIVYGRKALGRARLKPSVSLEGLLKYSEQFIPLDPVLSGCLPNNPWMSDDTTYWVL PPDTTCVFERDNMRMASTSLAKQ---------------------------------KTAEYYKTEIYYCGKALVRARVKSSTSLEGFVRHCDLYASHDPIMSGCLPNNPWISQDPSLWSL PPGQINSLESSIKRNCRMTTYNV---K------------------------K---RRTLEDYKKQIAYLQRALQRPRLKTSKAIHYLIQHCEQYAEHDPIISGAQPSNPWVTDDTTMWVL PPGQTNCLEADIRRKCKNSQPAR---T------------------------K---KPSPEEFKKQVTYLQNRLSKMTIKTSKAIDVLIRYCERFAEHDPFISGAQPSNPWISDDSTMWTL VPGCVNTTEMDIRKCRRLKNPQK---VKKSVYGVTEESQAQSPVHVLSQPIR---KTTKEDIRKQITFLNAQIDRHCLKMSKVAESLIAYTEQYVEYDPLITPAEPSNPWISDDVALWDI VPGCVNTTEVDIKKSSRMRNPHK---TRKSVYGLQNDIRSHSPTHTPTPETK---PPTEDELQQQIKYWQIQLDRHRLKMSKVADSLLSYTEQYLEYDPFLLPPDPSNPWLSDDTTFWEL .......250.......260.......270.......280.......290.......300.......310.......320.......330.......340.......350.......360

280 280 281 281 267 285 282 256 282 282 282 283 283 283 284 282 282 282 282 285 282 282 282 284 284 290 284 284 263 263 240 240 286 263 284 282 282 281 308 277 281 280 281 265 265 251 252 267 300 140 319 316

RGS9_Human_NP-001159405 RGS9_Cattle_NP-776595 RGS9_Koala_XP-020856566 RGS9_Opossum_XP-007482817 RGS9_Pigeon_XP-013225676 RGS9_Quail_XP-015735540 RGS9_Ground-tit_XP-014111246 RGS9_Turtle_XP-014424786 RGS9_Python_XP-007421674 RGS9_Garter_snake_XP-013926935 RGS9_X.laevis_XP-018090999 RGS9_Florida_gar_21856-1-2 RGS9_Bowfin_26690-1-1 RGS9_Zebrafish_NP-001314729 RGS9_Medaka_XP-004071947 RGS9_Reef_shark_51239-1-1 RGS9_Bamboo_shark_45486-8-1 RGS9_Bluespot_ray_37076-2-1 RGS9_Western_ray_41046-8-8 RGS9_Elephant_shark_XP-007886905 RGS9_M.mordax_34174-1-2 RGS9_G.australis_56905-1-2 RGS9_E.cirrhatus_137654-1-3 RGS11_Human_NP-899180 RGS11_Cattle_NP-001069282 RGS11_Koala_XP-020862219 RGS11_Opossum_XP-001372371 RGS11_Chicken_XP-001231808 RGS11_Pigeon_XP-005508486 RGS11_Ground-tit_XP-014107999 RGS11_Turtle_XP-007066592 RGS11_Alligator_XP-014457643 RGS11_Anole_XP-003227697 RGS11_Nanorana_XP-018423234 RGS11_Xenopus_NP-001096497 RGS11_Florida_gar_29521-1-2 RGS11_Bowfin_26311-1-2w1-5 RGS11_Zebrafish_XP-005170921 RGS11_Medaka_XP-011484761 RGS11_Reef_shark_50711-1-1 RGS11_Bamboo_shark_41815-1-3 RGS11_Bluespot_ray_32308-1-2 RGS11_Western_ray_41178-3-3 RGS11_M.mordax_39562-1-2 RGS11_G.australis_47497-1-2 RGS11-Partial-Trimmed_P.marinus_S4RW55 RGS11-Partial-Trimmed_L._camtschaticum_JL5627 RGS11_E.cirrhatus_136920-1-2 RGS_S.kowalevskii_XP-006813443 RGS-Partial_S.purpuratus_XP-011673106 RGS6_Human_NP-001191345 RGS7_Human_NP-002915

. * . *: * :.: : * * : :* * * *:: . NAKLVEIPTKMRVERWAFNFSELIRDPKGRQSFQYFLKKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGKTMDITVKGLKHPHRYVLDAAQTHIYMLMKKDS NAKLVDIPTKMRVERWAFNFSELIRDPKGRQSFQHFLRKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGKTMDITVKGLKHPHRYVLDAAQTHIYMLMKKDS NAKLVEIPTKMRVERWAFNFSELIRDPKGRQSFQFFLKKEFSGENLGFWEACEDLKYGDQSKVKEKAQEIYKLFLAPGARRWINIDGKTMDITVKGLKHPHRYVLDAAQTHIYMLMKKDS NAKLVDIPTKMRVERWAFNFSELIRDPKGRQSFQFFLKKEFSGENLGFWEACEDLKYGDQSKVKEKAQEIYKLFLAPGARRWINIDGKTMDITVKGLKHPHRYVLDAAQTHIYMLMKKDS NAKLVEVPTRMRVERWAFNFSELIRDPKGRQNFQLFLKKEFSGENLSFWEACEDLKYGDQSNIKEKAEEIYKLFLAPGARRWINIDGTTMGITVRGLKHPHRYVLDAAQTHIYMLMKKDS NAKLVEIPTRMRVERWAFNFSELIRDPKGRQNFQLFLKKEFSGENLSFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGTTMGITVKGLKHPHRYVLDAAQTHIYMLMKKDS NAKLVEVPTRMRVERWAFNFSELIRDPKGRQNFQLFLKKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGKTMGITVKGLEHPHRYVLDAAQTHIYMLMKKDS NAKLVEIPTRMRVERWAFNFNELIRDPKGRQNFQLFLKKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGTTMGITVRGLKHPHRYVLDAAQTHIYMLMKKDS NAKLVEIPTKARVEKWALNFSELMKDPKGRQSFQLFLKKEFSGENLGFWEACEDLKYGDQSKVKEKAEEIYKTFLAPGARRWINIDSTTMGITVRGLKNPHRYVLDAAQTHIYMLMKKDS NAKLVEIPTKARVEKWALNFRELMKDPKGRQNFQLFLKKEFSGENLGFWEACEDLKYGDQSKAKEKAEEIYKTFLAPGARRWINIDGTTMAITVKGLKNPQRYVLDAAQTHIYMLMKKDS NAKLVELPTKLRVERWGFNFSELIRDPKGRQSFQLFLKKEFSGENMSFWEACEDLKYGDQSKVKEKAEEIYKLFLAPGARRWINIDGKTMDITVKGLKHPHRYVLDAAQTHIYMLMKKDS NMPMVENPTKLRVERWTFSFKELLSDPRGRQDFQLFLKKEFSGENLAFWEACEDLKFGDQTKVKEKAEEIYKTFLAPGARRWINIDGKTMDITVKGLKHPHRYVLDAAQTHIYMLMKKDS NMPLAEVPTKLRVERWTFNFRELLSDPRGRQDFQLFLKKEFSGENLAFWEACEDLKYGDQTKVKEKAEEIYKTFLAPGARRWINIDGKTMDITVKGLQHPHRYVLDAAQTHIYMLMKKDS NTDDIEVPTKMRVERWSFSFYELLNDPRGRADFKVFLKKEFSGENLAFWEAAEELKFGSAATMSEKAEQVFKTFLAPGAPRWINIDGRTMGLTVKGLEHPHRYVLEAAQTHIFMLMKKDT NMRRVEVPTKMRVERWSFSLFELLTDLRGRDDFKIFLKKEFSGENLAFWEAAEELKWGASSSMSAKAETIFKTFLAPGAPRWINIDGRTMGLTVKGLDHPHRYVLEAAQTHVFMLMKKDT NTPMVDNPTKLRVERWTFSFTELMRDPKGRQSFELFLRKEFSGENLAFWEACEDLKYGEQSKVKEKAEEIYKTFLAQGARRWINIDGRTMEITVKGLKHPHRYVLDAAQTHIYMLMKKDS NTPMADNPTKLRVERWTFSFAELMRDLRGRQNFELFLKKEFSGENLAFWEACEDLKHGEQSKVKEKAEEIYKTFLAQGARRWINIDGRTMEITVKGLQHPHRYVLDAAQTHIYMLMKKDS NTPMVENPTKLRVERWTFGFRELVRDPRGRQNFELFLKKEFSGENLAFWEACEDLKYGEQSKVKEKAEEIYKTFLAQGARRWINIDGRTMEITVKGLQNPHRYVLDAAQTHIYMLMKKDS NAPMVDNPTKLRVERWTFSFAELMRDPRGRQNFEFFLKKEFSGENLAFWEACEDLKYGEQSKVKEKAEEVYKTFLTQGARRWINIDGRTMEITVKGLQHPHRYVLDAAQTHIYMLMKKDS NIPMIDQPTKLRVERWTFSFPELIRDPRGRQDLQLFLKKEFSGENLAFWEACEDLKYGDQSKVKEKAEEIYKTFLAPGARRWINIDGKTMEITVKGLQHPHRYVLDSAQTHIYMLMKKDS NAPSVDVPTRLRVERWAFSFYELLRDPRGRREFQAFLKKEFSGENLAFWESCEDLKHGDQSKAKEKAEEIYKLFLAQGARRWINIDGKTMEATVRGLQCPHRYALDAAQTHIYMLMKKDT NSPSVEVPTRLRVERWAFSFRELLRDPRGRREFQAFLKKEFSGENLAFWEACEDLKHGDQGKAKEKAEEIYKLFLAQGARRWINIDGKTMDITVRGLQCPHRYVLDPAQTHIYMLMKKDT NAPNVGNPCRLRVERWAFGFGEILSDAYGLQEFHNFLKKEFSAENLDFWEACQELRYGDQVNMKEKAEKINKLYLAQGAQKWINIDGRTMGKTLSGLKCPHRYMLDSAQAHIYMLMKKDS NAPTVAAPTKLRVERWGFSFRELLEDPVGRAHFMDFLGKEFSGENLSFWEACEELRYGAQAQVPTLVDAVYEQFLAPGAAHWVNIDSRTMEQTLEGLRQPHRYVLDDAQLHIYMLMKKDS NAPSAAVPTRLRVEQWAFSFRELLEDPVGRAHFMDFLGKEFSAENLSFWEACEELRHGGQAQVPALVDAVYQQFLAPGAARWVNIDSRTMEQTLAGLTQPHRHVLDDAQRHIYLLMKKDS NAPTVTTPTKLRVERWAFNFGELLGDPLGHIQFMEFLKKEFSAENLSFWEACEDLRYGEQSKIAESVDSVYQQFLAPGATRWVNIDSKTMEKTLEGIRRPHRYVMDDAQMHIYMLMKKDS NAPTVPTPTKLRVERWAFNFRELLGDPLGHIQFMEFLKKEFSAENLSFWEACEDLRYGEQSKITESVDSVYQQFLAPGATRWVNIDSKTMEKTLEGIRRPHRYVMDDAQMHIYMLMKKDS NAPTVTTPTKLRVERWGFNFSELLMDPLGRTQLLEFLKKEFSAENLSFWEACEELRYGEQSRINEIVDSIYQQFLAPGATRWVNIDSKTMERTLEGIKTPHRYVMDDAQMHIYMLMKKDS NAPTVPTPTKLRVERWGFSFSELITDPLGRAQLLEFLKKEFSAENLSFWEACEELRYGEQSRIAEIVDSIYQQFLAPGATRWVNIDSKTMERTLEGIKTPHRYVMDDAQMHIYMLMKKDS NAPTVTTPTKLRVERWGFNFSELINDPLGRAQLLEFLKKEFSAENLSFWEACEELRYGEQSRIAEIVDSIYQQFLAPGATRWVNIDSKTMERTLEGIKTPHRYVMDDAQMHIYMLMKKDS NAPTVTTPTKLRVERWGFSFSELLGDTLGRRQLLEFLKKEFSAENLSFWEACEELRYGEQAHIADIVDSIYQQFLAPGATRWVNIDSKTMERTLEGIKTPHRYVMDDAQMHIYMLMKKDS NAPTVMTPTKLRVERWGFSFSELLGDALGRAQFMEFLKKEFSAENLSFWEACEELRYGEQSRIAEIMDSIYQHFLAPGATRWVNIDSKTMERTLEGLKTPHRYVMDDAQMHIYMLMKKDS NAPTVTAPTKLRVERWSFGFSELIGDPLGRVQLLEFLKKEFSAENLSFWEACEELRYGEQGRIAEIVDSIYQQFLAPGATRWVNIDSKTMERTLEGIKTPHRYVMDDAQMHIYMLMKKDS NAPMVPMPTKLRLDRWTFNFMEMLNDPRGRKEFLQFLEKEFSAENLLFWEACEDLRYGEQSKNISTVQSVYEQFLAPGAIRWVNIDSKTMEKTLEGIKNPHRYVLDDAQMHIYMLMKKDS NAPMVAMPSKLRVDRWTFNFMELLNDPRGRKEFLQFLEKEFSAENLCFWEACEELRYGEQSKIASTVESVYQQFLAPGATRWVNIDSKTMEKTLEGIKNPHRYVLDDAQMHIYMLMKKDS NADLVATPTKMRVERWGFSFPELLNDPLGRREFHQFLEKEFSAENLCFWEACEDIRHGESSKIAQKVEEVYQHFLAPGASKWVNIDSKTMEKTLQGVKTPHRYTMDEAQMHIYFLMKKDS NADLVATPTKMRVERWAFSFPELLNDPLGRREFRQFLEKEFSAENLCFWEACEDIKHGENSKIPQKVEEVYQHFLAPGASRWVNIDSKTMEKTLLGIKNPHRFVMDDAQMHIYFLIKKDS NSEAVTVPTKLRVERWSFSFTELLDDPMGRKEFLIYLEKEFSAENLCFWQACEDVRHGESSKIPEKVDEVYKQFLAPGASRWVNIDSKTMEKTLEGLKRPHRFVLDDAQMHIYFLMKKDS NTEVVPVPTRLRVERWSFSFMELLNDAMGRQEFMLYLEKEFSAENLYFWQACEEIRHGESSKIVEKVEEVYKNFLAPGAARWVNIDSKTMDKTLEGIKQPHRFVLDDAQMHIYFLMKKDS NADLVTIPTRLRVERWAFNFGELLHDPLGRQQFRFFLEREFSAENLSFWEACEDVQYGEQSKVEEKVEGIFEQFLAAGASKWVNIDSKTMEKTLDGLQIPHRYVFEDAQMHIYFLMKKDS NADLVTIPTRLRVERWAFNFSELLHDPLGRQQFRYFLEREFSAENLSFWEACEDVQYGEQSKVDEKVETIFEQFLAAGASKWVNIDSKTMEKTLDGLQIPHRYVFEDAQMHIYMLMKKDS NADLVAVPTRLRVERWAFSFQELLKDPLGRQEFRRFLEKEISAENLSFWEACEDVQYGEQSRVEEKVETIFEQFLAAGASKWVNIDSKTMEKTLDGLQMPHRYIFEDAQMHIYMLMKKDS NADLVAVPTRLRVERWAFSFQELLEDPLGRQEFRRFLEKEISAENLSFWEACEDVQYGEQSKVEEKVETIFEQFLAAGASKWVNVDSKTMEKTLDGLQMPHRYVFEDAQMHIYMLMKKDS NATMVEVPLRLRVERWAFSFQELIQDLRGRKEFQEFLCKEFSGENLNFWEASEDLLHGDCTRVQEKVEQVYTDFLAPGAKRCINIDAKTMAVTIEGLKSPHRYVLEAAQTHIYMLMKKDS NATMVEVPVRLRVECWAFSFQELIQDLRGRKEFQEFLCKEFSGENLSFWEACEDLLYGDCTRVHEKVEQVYTDFLAPGAKRCINIDAKTMAVTMEGLKSPHRYVLEAAQTHIYMLMKKDS NATMVEVPLRLRVERWAFSFQELIQDLRGRKEFQEFLCKEFSGENLSFWEACEDLLYGDCTKLHEKVEQVYTDFLAPWAKRCINIDAETMAVTLEGLKSPHRYVLEAAQAHIYMLMKKDS NDTMVEVPLRLRVERWSFSFQELIQDLRGRKEFKEFLCMEFSGEQISV-----------------------------------------------------------------------HATMVEVPAKQRVECWAFSFRVLLNDQRGRQELREFLLKEFSAENLSFWESCEDLKSCELSNVKEKAEEIFGEFLSPGAKHWINLDGRTMAMTVAGMKQPCRFVFEEAQGHIYLLMKKDC NTVLVDIPTERRVKRWAISLKELLSDATGRHEFEMFLRKEYSQENIRFWQACEELKFSPQSQIEQNVQEIYKEFLATGARCEINVDGKTVEVTQKALQKPCRFTFEAAQTHIFMLMKKDS NTMLVDTPTERRVRRWAISFRELLSDVTGKHEFEKFLQKEYSQENIRFWSACETLKYAPQSMVPEMVHEIYRTFLAPGAPCEINVDGRTMELTQQSLRKPNRFAFEAAQQHIFLLMKKDS EM--SKEPSQQRVKRWGFSFDEILKDQVGRDQFLRFLESEFSSENLRFWLAVQDLKKQPLQDVAKRVEEIWQEFLAPGAPSAINLDSHSYEITSQNVKDGGRYTFEDAQEHIYKLMKSDS EA--SKEPSQQRVKRWGFGMDEALKDPVGREQFLKFLESEFSSENLRFWLAVEDLKKRPIKEVPSRVQEIWQEFLAPGAPSAINLDSKSYDKTTQNVKEPGRYTFEDAQEHIYKLMKSDS .......370.......380.......390.......400.......410.......420.......430.......440.......450.......460.......470.......480

400 400 401 401 387 405 402 376 402 402 402 403 403 403 404 402 402 402 402 405 402 402 402 404 404 410 404 404 383 383 360 360 406 383 404 402 402 401 428 397 401 400 401 385 385 371 300 387 420 260 437 434

RGS9_Human_NP-001159405 RGS9_Cattle_NP-776595 RGS9_Koala_XP-020856566 RGS9_Opossum_XP-007482817 RGS9_Pigeon_XP-013225676 RGS9_Quail_XP-015735540 RGS9_Ground-tit_XP-014111246 RGS9_Turtle_XP-014424786 RGS9_Python_XP-007421674 RGS9_Garter_snake_XP-013926935 RGS9_X.laevis_XP-018090999 RGS9_Florida_gar_21856-1-2 RGS9_Bowfin_26690-1-1 RGS9_Zebrafish_NP-001314729 RGS9_Medaka_XP-004071947 RGS9_Reef_shark_51239-1-1 RGS9_Bamboo_shark_45486-8-1 RGS9_Bluespot_ray_37076-2-1 RGS9_Western_ray_41046-8-8 RGS9_Elephant_shark_XP-007886905 RGS9_M.mordax_34174-1-2 RGS9_G.australis_56905-1-2 RGS9_E.cirrhatus_137654-1-3 RGS11_Human_NP-899180 RGS11_Cattle_NP-001069282 RGS11_Koala_XP-020862219 RGS11_Opossum_XP-001372371 RGS11_Chicken_XP-001231808 RGS11_Pigeon_XP-005508486 RGS11_Ground-tit_XP-014107999 RGS11_Turtle_XP-007066592 RGS11_Alligator_XP-014457643 RGS11_Anole_XP-003227697 RGS11_Nanorana_XP-018423234 RGS11_Xenopus_NP-001096497 RGS11_Florida_gar_29521-1-2 RGS11_Bowfin_26311-1-2w1-5 RGS11_Zebrafish_XP-005170921 RGS11_Medaka_XP-011484761 RGS11_Reef_shark_50711-1-1 RGS11_Bamboo_shark_41815-1-3 RGS11_Bluespot_ray_32308-1-2 RGS11_Western_ray_41178-3-3 RGS11_M.mordax_39562-1-2 RGS11_G.australis_47497-1-2 RGS11-Partial-Trimmed_P.marinus_S4RW55 RGS11-Partial-Trimmed_L._camtschaticum_JL5627 RGS11_E.cirrhatus_136920-1-2 RGS_S.kowalevskii_XP-006813443 RGS-Partial_S.purpuratus_XP-011673106 RGS6_Human_NP-001191345 RGS7_Human_NP-002915

YARYLKSPIYKDMLAKAIEPQETTK----KSSTLPFMRRHLRSSPSPVILR-----QL-EEEAKAREAANT---VDITQVM-SKLDRRSQL-----------------------QKELPP YARYLKSPIYKEMLAKAIEPQGTTR----KSSSLPFMRRHLRSSPSPVILR-----QL-EEEAKAREAATT---VDITQVM-SKLDRRSQL-----------------------RKEPPP YARYLKSPIYKEMLAKAIEPQETSR----RSSSLPFRRRHLRSSPSPVILR-----QL-EEEAKAREAANT---VDITQVM-SKLDRRSQL-----------------------KKEIPP YARYLKSPIYKEMLAKAIEPQETSK----R-SSLPFMRRHLRSSPSPVILR-----QL-EEEAKAREAANT---VDITQVM-SKLDRRSQL-----------------------KKEIPP YGRYLKSPIYKEMLAKAVVPQETVK----KSSNFPFGRRHLRSSPSPVILR-----QQ-EEEAKAKEAATT---VDITQVM-SKLDRRSQL-----------------------QKD-PP YGRYLKSPVYKEMLAKAVVPQETVK----KSSSFPFGRRHLRSSPSPIILR-----QQ-EEEAKAKEAAAT---VDITQVM-SKLDRRSQL-----------------------KQE-PP YGRYLKSPIYKEMLAKAVVPQEGVK----KSTGL-FGRRHLRSSPSPIILR-----QQ-EEEAKAKEAANT---VDITQVM-SKLDRRNQP-----------------------QKEAPP YGRYLKSPIYKEMLAKAIVPQETVK----KSSSFPFARRHLRSSPSPVILR-----QQ-EEEAKAKEAATN---VDITQVM-SKLDRRSQL-----------------------KQE-PP YGRYLKSPIYKEMLSKAITPQEAVK----KSSNNPFVRKHLRSSPSPILLR-----QL-EEEARAKEAAAN---VDITQVM-SKLDRRSQL-----------------------RQEAPP YGRYLKSPVYKEMLSKAIVPQEVVK----KSSYNPFARKHLRSSPSPILLR-----QL-EEEAKAKEAATN---VDITQVM-SKLDRRSQL-----------------------KQEAPP YARYLKSPIYKEILAKAVVPQETKK------SNFPFMRRHRRSSPSPVILR-----QL-EEEARAKEAANT---VDITQVM-SKLDRRNQL-----------------------KQETPK FGRYMKSPIFKETMKKAIVPDETKL----NEGKFGFNRKHRRSSPSPVILR-----QL-EEEAKAKAAASAP--VDITQVM-SKLDRRNQL-----------------------KQEPPQ FGRYMKSPIFKDTMKKAIIPEETKV----SESKFGFSKKHRRSSPSPVILR-----QL-EEEAKAKAAANAP--VDITQVM-SKLDRRNQL-----------------------KQEPAK FYRYLKSPVYKDIQKKAISPAPHNF----TEAQLEQNMRNRRPSISPIITY-----QK-EQEEKAKAAAAAGP-VDIKKLMASKLDRK-------------------------------FFRYLKSPTYKDIQKKALNPEAHNF----SPAQLEQNAQNRNPGIDPIILW-----QQ-DEEEKAKAAAASAP-VDVKAMM-SKIDRKK------------------------------YPRFLRSPIYKETEGKALIPEETK-----QKSNFPFMKRHRRSSPSPVILR-----QL-ELEAKAKAAASGTKVVDITQVM-SKLDRRNQL-----------------------KQEPPK YPRFLRSPIYKEMQSKALIPEETK-----PKSNFPFMKRHRRSSPSPVILR-----QL-EQEARAKAAASGPKVVDITQVM-SKLDRRNQL-----------------------KQEPSK YPRFLKSPIYKETQNKALVPEETK-----QKSNFPFMRRQRRSSPSPVVLR-----QL-EEEARARAAASN-KVVDITQVM-SKLDRRHQL-----------------------KQEPPR YPRFLKSPIYKETQNKALVPEETK-----QKSNFPFMRRQRRSSPSPVVLR-----QL-EEEARARAAAST-KVVDITQVM-SKLDRRHQL-----------------------KQEPPK YPRYLKSPIYKETQAKALIPDQTK-----QKS-FPFMKRHRRSSPSPVLLR-----QL-EEEARARAAASGPKILDITQVM-SKLDRRNQL-----------------------KPEPPK FYRYLKSDLYKKLLAQAIVPRETDK------S----ARFARHTSPSPVSLR-----AE-QEEEARRAAAAGPPPADLTQVL-SQLDRRNRP-----------------------AK---FYRYLKSDLYKSLLARAVVPRETDK------S----ARFPRHASPSPLVIR-----AA-EEEEEARKAAAAGPPTDLTQVM-SKLDRRNQP-----------------------AK---YPRYLKCDLYKNLLINAVVPQETKI------STLPFVHPKRHSNPSPVVLR-----NE-EDTAPLIPLPSASEVSGFSSIF-SPRIYRGPP-----------------------AVGPGQ YPRFLKSDMYKALLAEAGIPLEMKR------RVFPFTWRPRHSSPSPAL-LPTPVEPTAACGPGGGD-------------G-VA-----------------------------------YPRFLKSDLYRDLLAEAVVPLETKR------RVFPFTRKPRHSSPSPAL-LP----ASASGEPVGGD-----------GDG-ET-----------------------------------YPRFLKSDMYKTLLAEAVIPPETKK------RVFPFMRKQRHSSPSPAL-LL----PS-EQEPKGEDKNKALASAASAGEG-EG-----------------------------------YPRFLKSDMYKTLLAEAVIPPETKK------RVFPFMRKQRHSSPSPAL-FL----PSGEQEPKGDDKGKALAPGTSAGEG-EV-----------------------------------YPRFLKSELYRNLLAEAVIPPETKK------RVFPFMRKQRHSSPSPAL-LL----PAGDTEAKGEEKSRSPAA--VPEE--ES-----------------------------------YPRFLKSELYRNLLAEAVIPPETKK------RVFPFMRKQRHSSPSPAL-LL----PAGDTE----EKSRGPTASPVPEE--ES-----------------------------------YPRFLKSDLYKNLLAEAIIPPETKK------RVFPFMRKQRHSSPSPAL-LL----PAGDAE----ERSRSPSTAAVPEE--ES-----------------------------------YPRFLKSELYRSLLAEAIVPPETKK------RVFPFMRKQRHSSPSPAL-LL----PTGEPEAKAEDQSKAVA---VPME---S-----------------------------------YPRFLKSDLYKSLLAEAIIPPETKK------RVFPFMRKQRHSSPSPALLLL----PGGEPEAKAEDKSRTPVAAPVPEE--ES-----------------------------------YPRFIKSDLYKNLLAEAIIPPETKR------RVFPFMRKPRHASPSPALQQL----PPGDSEGRAEPEQ---------PEG-GT-----------------------------------YPRYLKSEMYKNLLGQAFIPPETKK------GVFPFMRRQRHSSPSPAF-LF----PPGDQESKADGDSRVNSPAAMSQE---------------------------------------YPRYLKSDMYKNLLGQAFIPPETKK------GVFPFMRRQRHSSPSPAF-LL----PQGEQEGRTEP----LTPAALSQP---------------------------------------YPRFLKSDLYKNLLARAIVPQETKK------SVFPFMRRQRHSSPSPA-LVS----QPAEDDGKSKAA---PSSSGTVSQV-EE-----------------------------------YPRYLKSDLYKNMLARAIVPQETKK------SVFPFMRRQRHSSPSPA-LVS----HPTEDDGKAKAS---P--AGTVSQV-EE-----------------------------------FPRYLKSDLYKSLLARAIVPQETKK------SVFPFMRRQRHSSPSPA-LVT----HTTEDDGKAKNSNSVKSNSGTASRL--------------------------------------YPRYLKSDLYKNMLAKAIVPQETKK------SVFPFMRRQRHSSPSPAQAVA----QVTEEEGRHKGA---G-----------------------------------------------YPRYLKSDMYKNILARSVVPQETKK------CMIPFLRWYRNSSQSSA-QMF----YPKDGDGKGKDT--------TVSQV-QN-----------------------------------YPRYLKSDMYKNILARSIVPQETKR------SMVPFMRWHRYSNASSV-QMF----YPKDNDGKGKDA--------TVSQV-QN-----------------------------------YPRYLKSQMYKNLLESSIVPQESRK------SVFPFTKWHRQPNQNPS-QTP----RPKVSDGKDKAA-----PAGTVSQV-QN-----------------------------------YPRYLKSQIYKNLLATSIVAQESKK------SAFPFTRWHRQPSQNPA-QTP----RP---DGKDKAA-----TSATVSQV-QN-----------------------------------YPRYLKSDLYKTMLARAIVASAVNA------HSH-------------------------------------------------------------------------------------YPRYLKSDLYKTMLAGAIVPCAVNA------HPLSGTRSL-------------------------------------------------------------------------------YPRYLKSDLYKTMLAGAIVP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YPRYLKSEMYKSLLERAIVPQDNKR------RIFPLMMRPRLSSPPIT-LQG----I--------------------------------------------------------------YPRFLRSDAYKNLLAHSLQPSSKKKFFTFGNR-LKTAKVTPNASPQPLRRR-----GS-ANDMTHKAHNARHGLSLSTGNL-QDLDKSDEKINADFLYSDVYQGGESKENSPN-QSPTSN YQRFLKSDTYKGLLARAMQNGTKRKFFTFGGRFLRSAKVTPDVSPLGYPRR-----GSLGTDISDSLSDRPMTHSLSASNL-QD-----------FELANMYNGTENGHKTEEVYTSVKS YARFLRSNAYQDLLLAKKKPESEQG------RRTSLEKFTRSVGKSLAGKR-------------------------LTGLM-QS-----------------------------------YPRFIRSSAYQELLQAKKKSGNSMD------RRTSFEKFAQNVGKSLTSKR-------------------------LTSLA-QS-----------------------------------.......490.......500.......510.......520.......530.......540.......550.......560.......570.......580.......590.......600

483 483 484 483 469 487 484 458 485 485 483 487 487 480 481 487 487 486 486 489 478 478 486 467 465 481 476 473 450 450 427 432 470 452 469 471 469 471 487 461 465 467 465 413 419 391 300 433 531 363 489 486

RGS9_Human_NP-001159405 RGS9_Cattle_NP-776595 RGS9_Koala_XP-020856566 RGS9_Opossum_XP-007482817 RGS9_Pigeon_XP-013225676 RGS9_Quail_XP-015735540 RGS9_Ground-tit_XP-014111246 RGS9_Turtle_XP-014424786 RGS9_Python_XP-007421674 RGS9_Garter_snake_XP-013926935 RGS9_X.laevis_XP-018090999 RGS9_Florida_gar_21856-1-2 RGS9_Bowfin_26690-1-1 RGS9_Zebrafish_NP-001314729 RGS9_Medaka_XP-004071947 RGS9_Reef_shark_51239-1-1 RGS9_Bamboo_shark_45486-8-1 RGS9_Bluespot_ray_37076-2-1 RGS9_Western_ray_41046-8-8 RGS9_Elephant_shark_XP-007886905 RGS9_M.mordax_34174-1-2 RGS9_G.australis_56905-1-2 RGS9_E.cirrhatus_137654-1-3 RGS11_Human_NP-899180 RGS11_Cattle_NP-001069282 RGS11_Koala_XP-020862219 RGS11_Opossum_XP-001372371 RGS11_Chicken_XP-001231808 RGS11_Pigeon_XP-005508486 RGS11_Ground-tit_XP-014107999 RGS11_Turtle_XP-007066592 RGS11_Alligator_XP-014457643 RGS11_Anole_XP-003227697 RGS11_Nanorana_XP-018423234 RGS11_Xenopus_NP-001096497 RGS11_Florida_gar_29521-1-2 RGS11_Bowfin_26311-1-2w1-5 RGS11_Zebrafish_XP-005170921 RGS11_Medaka_XP-011484761 RGS11_Reef_shark_50711-1-1 RGS11_Bamboo_shark_41815-1-3 RGS11_Bluespot_ray_32308-1-2 RGS11_Western_ray_41178-3-3 RGS11_M.mordax_39562-1-2 RGS11_G.australis_47497-1-2 RGS11-Partial-Trimmed_P.marinus_S4RW55 RGS11-Partial-Trimmed_L._camtschaticum_JL5627 RGS11_E.cirrhatus_136920-1-2 RGS_S.kowalevskii_XP-006813443 RGS-Partial_S.purpuratus_XP-011673106 RGS6_Human_NP-001191345 RGS7_Human_NP-002915

-K--------------------------------------------------------------------------K--------------------------------------------------------------------------K--------------------------------------------------------------------------K--------------------------------------------------------------------------K--------------------------------------------------------------------------K--------------------------------------------------------------------------K--------------------------------------------------------------------------K-------------------------------------------------------------------------AN-------------------------------------------------------------------------AK-------------------------------------------------------------------------P--------------------------------------------------------------------------T KT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QE--AQ---------------------------------------------------------------------QE--A----------------------------------------------------------------------PE--AQ---------------------------------------------------------------------PE--AQ---------------------------------------------------------------------EEPPKQ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SNAISCNPMQN----------------------------------------------------------------G ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KKFIIGSPITSRKKVNFTTQEHVNMSLEVPKRYPVNSPDSPEVDTNSKPLSFPVPSKKSLITPWEEQDIPTDSSLN SRFASLQPMNSTNLKRHGSLG------------------------------------------------------G ---------------------------------------------------------------------------S ---------------------------------------------------------------------------Y .......610.......620.......630.......640.......650.......660.......670......

484 484 485 484 470 488 485 459 487 487 485 489 487 480 481 491 490 490 490 495 478 478 498 467 465 481 476 473 450 450 427 432 470 452 469 471 469 471 487 461 465 467 465 413 419 391 300 433 607 385 490 487

Gb5L_Human_NP-057278 Gb5L_Cattle_XP-010807658 Gb5L_Opossum_XP-007480030 Gb5L_Ground_tit_XP-005521542 Gb5L_Crow_XP-008638229 Gb5L_Falcon_XP-005241495 Gb5L_Turtle_XP-007056427 Gb5L_Alligator_XP-019333396 Gb5L_Xenopus_XP-012814636 Gb5L_Nanorana_XP-018431551 Gb5L_Coelacanth_XP-014351988 Gb5L_Florida_gar_16504-1-1 Gb5L_Bowfin_22627-1-1 Gb5L_Zebrafish_NP-957040 Gb5L_Medaka_XP-011491247 Gb5L_Reef_shark_40872-1-1 Gb5L_Bamboo_shark_37645-2-1 Gb5L_Bluespot_ray_28709-1-1 Gb5L_Western_ray_37263-2-2 Gb5S_Elephant_shark_XP-007904318 Gb5L_M.mordax_41947-2-2 Gb5L_G.australis_57438-2-2 Gb5L-Partial_P.marinus_S4RZE3 Gb5L_E.cirrhatus_130391-1-2 Gb5_S.kowalevskii_XP-002740524 Gb5_S.purpuratus_XP-792481

. : : *:: * * ** :* :** **:***: *::: : * : :*.** MCDQTFLVNVFGSCDKCFKQRALRPVFKKSQQLSYCSTCAEIMATEG-LHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRR M-DQTFLVNVFGSCDRCFKQRALRPVFKKSEQLNYCSLCVEIMAADG-LHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRR MCDQTFLVNVFGSCDKCFKQRALRPVFIRSQQLSYCSVCAALMAAEG-LHESETLASLKSEAESLKSRLEEERAKLHDVELHQVAERVEALGQFVMKTRR M-DQTFLAIVFGPCDKCSKEKPLRPVSVKSEQRSYCSLCVEMMATEG-LHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRR M-DQTFLAIVFGPCDKCSKDKPLRPVSVKSEQRSYCSLCVAMMATEG-LHENETLASLKSEAESLKGKLEEERAKLHDVEVHQVAERVEALGQFVMKTRR MCDQTFLAIVFGPCDKCSKEKPLRAVYIKSEQLSYCSLCVEMMATEG-LHENETLASLKNEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRR MCDQTFLAIVFGPCDKCSTEKPLRATYIKSEQLNYCSLCVEMMATEG-LHENETLASLKSEAESLKGRLEEERAKLHDVELHQVAERVEALGQFVMKTRR MCDQTFLATVFGPCDKCSNLKPLRAIYIKSSQLDYCSLCVEMMATEG-LHENETLASLKSEAESLKGKLEEERAKLHDVELHQVADRVEALGQFVMKTRR MCDQTFLTVLFGPCGKCKRERPLRTIYVKAEPVTYCSLCVEMMATEG-LQENETLASLKNEAESLKEKLEEERAKLHDVELHQVAERVESLGQFVMKTRR MCDQTFLVVLFGPCAKCTRERPLRTIYLKAEPVTLCSLCVELMATEG-LQENETLASLKNEAEGLKGKLEEERAKLHDVELHQVAERVESLGQFVMKTRR MCDQTFLAVAFGPCEKCQQEKPLRNIYIKTEQVNYCTLCVELMATEG-LQENETLASLKNEAESLKGKLEEERAKLHDVELHQVADRVDGLGQFVMKTRR MCDQTFLAINFGPCDKCQKTKPLMNIYIKSEPINYCALCVELMATEG-LQESETLASLKNESETLKSKLEEERAKLHDVELHQVADRVEALGQFVMKTRR MCDQTFLATNFGPCEKCGKTKPLMNIYIKTDPINYCSLCVELMATEG-LQENETLASLKNESETLKSKLEEERAKLHDVELHQVADRVEALGQFVMKTRR MCDQTFLAITFGPCDKCSENKPLMNIYLKNEPINYCSLCVEMMACQG-LAKGETVQSLKAESESLKAKLEEERAKLHDVELHQVAEKMEALGQFVMKTRR MCDQTFMAATFGPCDSCGKASPLMNIYIKSEAINYCSFCVEMMACQQ-LQKGETLQSLKKESDSLKKKLEEERGKLNDVELHQVAEKVEVLSSLSIKTRR MCDQTFAAATFGPCEKCQEVGPLRNLYINEEQINYCSLCVEMMATEG-LQENETVSSLRDEAETLKGKLEEERAKLHDVELHQVAERTEALGQFVMKTRR MCDQTFAAATFGPCEKCQEVGPLRNLYIDTEQINFCSLCVEMMATEG-LQENESVSSLREEAETLKGKLEEERAKLHDVELHQVAERTEALGQFVMKTRR MCDQTFAAAVHGPCERCQEVGPLRNLYIKDEQINVCSLCVEMMATEG-LQENETVSSLRNEAEALKGKLEEERAKLHDVELHQVAERTEALGQFVMKTRR MCDQTFAAAVYGPCEKCQEIGPLRNLYINAEQINYCSLCVEMMATEG-LQENETVSTLRNEAEALKGKLEEERAKLHDVELHQVAERTEALGQFVMKTRR ------------------------------------------MATEA-LQENETVASLKDEAESLKSKLEEERAKLHDVELHQVAERAEALGQFVMKTRR MCDQTFLTTCFGQCDACCKERPLRSVLSKAQPRNVCTICAELMASEG-LQENETIDGLQGEAENLKIKLEEERAKLHDVELHQVAERVEALGQFVMKTRR MCDQTFLVICFGKCDVCSNERPLRGVFIQAQPRNVCTSCAEMMASEG-LQENETIDGLQGEAENLKIKLEEERAKLHDVELHQVAERVDALGQFVMKTRR MCDQTFLVVCFGQCDLCFKERPLRGVLINAQSQNVCTSCAELMASEG-LQENETIDGLQGEAENLKMKLEEERAKLHDVELHQVAERVEALGQFVMKTRR MSDQIFSAVNFGPCESCECVHALQEVWSHGKAYSLCTECKQLMASEV-LQEGETLSVLQQEAAALKARLEDERAKLHDVELRQVAERVEPLGQFVMKPRR -----------------------------------------MATTDA-AMKEESISGLSRELETLKAKLEEERHKLNDVELRLVSQRLELLPQFNMKPRR -----------------------------------------MAVTDGNAVKEETLTALTREAENLKQKLLEERHKLNDVEMYTVAQRLEPLPPLNLKPRR 1.......10........20........30........40........50........60........70........80........90.......100

99 98 99 98 98 99 99 99 99 99 99 99 99 99 99 99 99 99 99 57 99 99 99 99 58 59

Gb5L_Human_NP-057278 Gb5L_Cattle_XP-010807658 Gb5L_Opossum_XP-007480030 Gb5L_Ground_tit_XP-005521542 Gb5L_Crow_XP-008638229 Gb5L_Falcon_XP-005241495 Gb5L_Turtle_XP-007056427 Gb5L_Alligator_XP-019333396 Gb5L_Xenopus_XP-012814636 Gb5L_Nanorana_XP-018431551 Gb5L_Coelacanth_XP-014351988 Gb5L_Florida_gar_16504-1-1 Gb5L_Bowfin_22627-1-1 Gb5L_Zebrafish_NP-957040 Gb5L_Medaka_XP-011491247 Gb5L_Reef_shark_40872-1-1 Gb5L_Bamboo_shark_37645-2-1 Gb5L_Bluespot_ray_28709-1-1 Gb5L_Western_ray_37263-2-2 Gb5S_Elephant_shark_XP-007904318 Gb5L_M.mordax_41947-2-2 Gb5L_G.australis_57438-2-2 Gb5L-Partial_P.marinus_S4RZE3 Gb5L_E.cirrhatus_130391-1-2 Gb5_S.kowalevskii_XP-002740524 Gb5_S.purpuratus_XP-792481

**** .***.:**.:*:*::*:****** ****.:* ****.:*:* ******:***** :*** *******: *: ::: ::**::*****.* TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRLVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFEKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFEKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMASKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAVACG--GLDNKCSVYPLTFDKNENMASKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNENMAAKKKSVAMHTNY TLKGHGNKVLCMDWCRDKRRIVSSSQDGKVIVWDAYTTNKEHAVTMPCTWVMACAYAPSGCAVACG--GLDNKCSVYPLSLDKNENLASKKKSVAMHTNY VLKGHGNKVLCVDWCKDKRRLVSSSQDGKAIVWDAFTLNKEHGVTLPCTWVMACAYAPSGCAVACG--GLDNKCSVVPLSLDKNENLAAKKKSVAMHTNY TLKGHGNKVLCMDWCRDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNESMAAKKKSVAMHTNY TLKGHGNKVLCMDWCRDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAVACG--GLDNKCSVYPLTFDKNESMAAKKKSVAMHTNY TLKGHGNKVLCIDWCRDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNESMAAKKKSVAMHTNY TLKGHGNKVLCMDWCRDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLTFDKNETMAAKKKSVAMHTNY TLKGHGNKVLCMDWCRDKRRIVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAVACG--GLDNKCSVYPLTFDKNESMAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRLVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLSTDKNENLAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRLVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLSMDKNENLAAKKKSVAMHTNY TLKGHGNKVLCMDWCKDKRRLVSSSQDGKVIVWDAFTTNKEHAVTMPCTWVMACAYAPSGCAIACGDRGLDNKCSVYPLSMDKNENLAAKKKSVAMHTNY TLKGHGNKVLCMDWCRDKRRLVSSSQDGKVIVWDGFTCNKEHAVTMPCTWVMACAYAPSGCAIACG--GLDNKCSVYPLSFDKNENMAAKKKSVAMHTNY VLKGHQGKVLAMDWSQDKRHVVSSSQDGKMIVWDAFTTNKEHAVTMPTTWVMACSYAPSGTMVACG--GLDNKCSIYQLSLDEDV--ASKKRTVAMHTSY ILKGHQGKVLCLDWARDQRHMVTSSQDGKVIVWDAFTTNKEHAITMPTTWVMACAYAPSGTMVACG--GLDNKCSVYQLTQEEDLS-SSKKRAVAMHTSY .......110.......120.......130.......140.......150.......160.......170.......180.......190.......200

197 196 197 196 196 197 197 197 197 197 197 197 197 197 197 197 197 197 197 155 197 197 199 197 154 156

Gb5L_Human_NP-057278 Gb5L_Cattle_XP-010807658 Gb5L_Opossum_XP-007480030 Gb5L_Ground_tit_XP-005521542 Gb5L_Crow_XP-008638229 Gb5L_Falcon_XP-005241495 Gb5L_Turtle_XP-007056427 Gb5L_Alligator_XP-019333396 Gb5L_Xenopus_XP-012814636 Gb5L_Nanorana_XP-018431551 Gb5L_Coelacanth_XP-014351988 Gb5L_Florida_gar_16504-1-1 Gb5L_Bowfin_22627-1-1 Gb5L_Zebrafish_NP-957040 Gb5L_Medaka_XP-011491247 Gb5L_Reef_shark_40872-1-1 Gb5L_Bamboo_shark_37645-2-1 Gb5L_Bluespot_ray_28709-1-1 Gb5L_Western_ray_37263-2-2 Gb5S_Elephant_shark_XP-007904318 Gb5L_M.mordax_41947-2-2 Gb5L_G.australis_57438-2-2 Gb5L-Partial_P.marinus_S4RZE3 Gb5L_E.cirrhatus_130391-1-2 Gb5_S.kowalevskii_XP-002740524 Gb5_S.purpuratus_XP-792481

Gb5L_Human_NP-057278 Gb5L_Cattle_XP-010807658 Gb5L_Opossum_XP-007480030 Gb5L_Ground_tit_XP-005521542 Gb5L_Crow_XP-008638229 Gb5L_Falcon_XP-005241495 Gb5L_Turtle_XP-007056427 Gb5L_Alligator_XP-019333396 Gb5L_Xenopus_XP-012814636 Gb5L_Nanorana_XP-018431551 Gb5L_Coelacanth_XP-014351988 Gb5L_Florida_gar_16504-1-1 Gb5L_Bowfin_22627-1-1 Gb5L_Zebrafish_NP-957040 Gb5L_Medaka_XP-011491247 Gb5L_Reef_shark_40872-1-1 Gb5L_Bamboo_shark_37645-2-1 Gb5L_Bluespot_ray_28709-1-1 Gb5L_Western_ray_37263-2-2 Gb5S_Elephant_shark_XP-007904318 Gb5L_M.mordax_41947-2-2 Gb5L_G.australis_57438-2-2 Gb5L-Partial_P.marinus_S4RZE3 Gb5L_E.cirrhatus_130391-1-2 Gb5_S.kowalevskii_XP-002740524 Gb5_S.purpuratus_XP-792481

:* * * :** *:* LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQAFETHESDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQAFETHESDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCIQSFETHESDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQSFETHDSDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQSFETHDSDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCIQSFETHDSDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCIQSFETHESDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCIQSFETHESDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRTGQCIQSFETHESDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRTGQCIQSFETHESDINSVRYYPSGDAFA LSSCSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCIQSFETHESDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHAADVLSLDLAPSETGNTFVSGGCDKKAMVWDLRSGQCIQSFETHESDINSVRYYPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHAADVLCLDLAPSETGNTFVSGGCDKKAMVWDLRSGQCIQSFETHESDINSVRYYPSGDAFA LSSCSFTKSDMQILTSSGDGTCALWDVESGQLLQSFHGHSADVLSLDLAPSETGSTFVSGGCDKKANVWDMRSGQNVQSFETHDSDINSVKYYPSGDAFA VSGCTFTNSDMQLLTSSGDGTCALWDVESGQLLQSFHGHTADVLSLDLAPSETGNTFVSGGCDMKANVWDMRSGQNIQSFESHESDINCVRYYPSGDAFA LSACSFTNSDMQILTSSGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGHTFVSGGCDKKAMVWDMRSGQCIQSFETHDSDINSVRYFPSGDAFA LSACSFTNSDMQILTSSGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGHTFVSGGCDKKAMVWDMRSGQCIQSFETHESDINSVRYFPSGDAFA LSACSFTNSDMQILTSSGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGHTFVSGGCDKKAMVWDMRSGQCIQSFETHESDINSVRYFPSGDAFA LSACSFTNSDMQILTSSGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGHTFVSGGCDKKAMVWDMRSGQCIQSFETHESDINSVRYFPSGDAFA LSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGHTFVSGGCDKKAMVWDMRSGQCIQSFETHESDINSVRYYPSGDAFV LSACCFTNSDMQILTSSGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRTGQCVQCFETHESDINSVRYYPSGDAFA LSACCFTNSDMQILTSSGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRTGQCVQCFETHESDINSVRYYPSGDAFA LSACCFTNSDMQVL-------------------------------------------------------------------------------------LSACCFTNSDMQLLTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFISGGCDKKALVWDIRTGQCVQCFETHESDINSVRFYPSGDAFA MSCCSFTNSDQQILTGSGDSTCALWDVESGQLLQSFHGHNADVMSLDLSPSESGNTFVSGGCDKQALVWDMRTGQCVQSFDGHESDINAVRFFPSGDAFA VSCCTFMNSDQQILTGSGDSTCALWDVESGQLLQSFHGHNADAMALDLSPSEACNTFVSGGCDKQALVWDMRTGQCVQSFEGHDSDINAVRFFPSGDGFA .......210.......220.......230.......240.......250.......260.......270.......280.......290.......300

SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGARVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFGGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDASCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDASCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKDSIIFGASSVDFSLSGRLLFAGYNDYNINVWDVLKGTRVAILFGHENRVSTVRVSPDGTAFCSGSWDNTLRIWA SASDDATCRFYDLRADREVAVYQKDSVIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSRVRVSPDGTALCSASWDNTLRIWA SGSDDATCRLYDLRADREVAIYSKDSIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKDSIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKDSIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA SGSDDATCRLYDLRADREVAIYSKDSIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSTLRVSPDGTAFSSGSWDHTLRIWA SGSDDATCRLYDLRADREVAVYSKDSIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRIWA TGSDDATCRLYDLRADREVAVFSKESIIFGASSVDFSLSGRLLFSGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCTGSWDHTLRVWA TGSDDATCRLYDLRADREVAVFSKESIIFGASSVDFSLSGRLLFSGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCTGSWDHTLRVWA -------------------------------------------------------------------------------------------------TGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGTRVAILFGHENRVSCLRVSPDGTAFCSASWDHTLKIWA TASDDATCRLYDLRADREVNIYTKESILFGATCLDFSLSGRLLFVGYNDYTVNIWDTLKGIRLAILYGHENRVSCLSVSPDGTSLCTGSWDYTLRVWA TASDDASCRMYDLRADREISCYSKESIIFGATSLDFSLSGRLLFVGYNDYSVNIWDTLKGHRLTMLYGHENRVSCLRVSPDGTAMCTGSWDYTLRIWA .......310.......320.......330.......340.......350.......360.......370.......380.......390........

297 296 297 296 296 297 297 297 297 297 297 297 297 297 297 297 297 297 297 255 297 297 213 297 254 256

395 394 395 394 394 395 395 395 395 395 395 395 395 395 395 395 395 395 395 353 395 395 213 395 352 354

R9AP_Human_NP-997274 R9AP_Cattle_NP-001159988 R9AP_Degu_XP-004636749 R9AP_Opossum_XP-007474849 R9AP_Tasmanian_devil_XP-003757239 R9AP_Koala_XP-020840579 R9AP_Ground-tit_XP-014110984 R9AP_Quail_XP-015729517 R9AP_Golden_eagle_XP-011572427 R9AP_Turtle_XP-007058713 R9AP_Alligator_XP-014451063 R9AP_Garter_snake_XP-013923971 R9AP_Gecko_XP-015270350 R9AP_Anole_XP-016852891 R9AP_Xenopus_XP-004913611 R9AP_Nanorana_XP-018425514 R9AP_Coelacanth_XP-006005212 R9AP_Florida_gar_28835-2-1 R9AP_Bowfin_1668-1-1 R9AP_Zebrafish_XP-005169975 R9AP_Asian_bonytongue_XP-018594265 R9AP_Reef_shark_40575-1-1 R9AP_Bamboo_shark_37642-1-1 R9AP_Bluespot_ray_25945-2-2 R9AP_Western_ray_30726-1-1 R9AP_Elephant_shark_XP-007887765 R9AP_M.mordax_41679-5-1 R9AP_G.australis_56404-2-1 R9AP_L.camtschaticum_JL14914 R9AP_E.cirrhatus_138946-5-1 R9AP-B_Xenopus_XP-002941417 R9AP-B_Nanorana_XP-018419161 R9AP-B-Partial_Coelacanth_XP-006013654 R9AP-B_Florida_gar_25574-1-1 R9AP-B_Zebrafish_NP-001314844 R9AP-B_Asian_bonytongue_XP-018613071 R9AP-B_Reef_shark_612-1-1 R9AP-B_Bamboo_shark_45543-9-1 R9AP-B_Bluespot_ray_37118-18-1 R9AP-B_Western_ray_39739-1-1 R9AP-B_Elephant_shark_XP-007902235 R9AP-like_Degu_XP-004636181 R9AP-like_Opossum_XP-007475029 R9AP-like_Tasmanian_devil_XP-003759057 R9AP-like_Koala_XP-020821390 R9AP-like_Quail_XP-015737481 R9AP-like_Ground-tit_XP-005524972 R9AP-like_Turtle_XP-007067829 R9AP-like_Alligator_XP-014450013 R9AP-like_Coelacanth_XP-005986755 R9AP-like_Xenopus_NP-001011277 R9AP-like_Nanorana_XP-018419805 R9AP-like_Spotted_gar_XP-006639786 R9AP-like_Zebrafish_NP-001018364 R9AP-like_Asian_bonytongue_XP-018611369 R9AP_S.kowalevskii_XP-006814063 R9AP_S.purpuratus_XP-011683108 R9AP_S.purpuratus_XP-011666321

: *: . M------------------------------------AREE-----------------------------------------------------CKALLDGLNKTTACYHHLVLTVGGSADSQNLRQELQ M------------------------------------AKEE-----------------------------------------------------CKALLDALNKATACYHHLVLTIGGSADSQNLREELQ M------------------------------------AREE-----------------------------------------------------SKALLDAFNKTTACYHHLVLTVGGSADSQNLREELQ M------------------------------------AKEE-----------------------------------------------------CKALLDALNRVTACYRHMVLSVGGTSDSQNLREELK M------------------------------------AKEE-----------------------------------------------------CKVLLDALNRVTACYRHMVLSVGGTADSQNLREELK M------------------------------------AKEE-----------------------------------------------------CKALLDALSRVTACYRHMVLSVGGTADSQNLREELK M------------------------------------VKEE-----------------------------------------------------CKALLDALNKVTACYRHMVLTIGGTSDSQNLREELK M------------------------------------VKEE-----------------------------------------------------CKALLDALSRVTACYRHMVLTIGGTSDSQNLREELK M------------------------------------VKEE-----------------------------------------------------CKALLDALNKVTACYRHMVLTIGGTSDSQNLREELK M------------------------------------VKEE-----------------------------------------------------CKALLDALSKVTACYRHMVLTIGGTSDSQNLREELK M------------------------------------VKEE-----------------------------------------------------CKALLDALNKVAACYRHMVLTIGGTADSQNLREELR M------------------------------------AKEE-----------------------------------------------------CKALLDALSKVTACYRHLVLTVGGAADSQELREELK M------------------------------------VKEE-----------------------------------------------------CKALLDALNKVTACYRQLVLTIGGTSDSQNLREELK M------------------------------------AKEE-----------------------------------------------------CKALLDALSKVTACYRHLVLTIGGASDSQNLREELQ M------------------------------------LKEE-----------------------------------------------------CKALLDALNKVTACYRHLVLTIGGTSDSQNLREELK M------------------------------------PAEE-----------------------------------------------------CRALLDALNKVTACYRHLVLTIGGTSDSQNLREELK M------------------------------------VKDE-----------------------------------------------------CKTLLDALNKVTACYRHMVIGVGGTSDSQNLREELK M------------------------------------GKEE-----------------------------------------------------CKTLLDALNKVTACYRHLVVAVGGTSDSQNLREELK M------------------------------------GKEE-----------------------------------------------------CKTMLDALNKVTACYRHLVIAVGGTSDSQNLREELK M------------------------------------GKDE-----------------------------------------------------CKTMLDALNKVTACYRHLVTALGSTSDSQNLREELK M------------------------------------GKEE-----------------------------------------------------CKTMLDALNRVTACYRHLVITLGSTSDSQNLREELR -------------------------------------MKDE-----------------------------------------------------CKALLDALNKVSACYRHLVICAGGTSDSQNLREELK M------------------------------------VKDE-----------------------------------------------------CKTLLDALNKVSACYRHLVICAGGSSDSQNLREELK M------------------------------------VNDE-----------------------------------------------------CKTLLDALNKVSACYRHLVICAGGTSDSQNLREELK M------------------------------------VKDE-----------------------------------------------------CKTLLDALNKVSACYRHLVICAGGTSDSQNLREELK M------------------------------------VRDE-----------------------------------------------------CKTLLDALNKVSACYRHLVVCVGGTSDSQNLREELR MA-----------------------------GASSAETDAQ-----------------------------------------------------CAALVQSLNKVTACHHHLAVSLGGSADCARLREELR M----------------------------------AETDAQ-----------------------------------------------------CAALVQSLNKVTACHHHLAVSLGGSADCARLRDELR MA-----------------------------TEGAAESDSQ-----------------------------------------------------CAALVQSLNKVTACHHHLAVSLGGSADCARLHEELR MP-----------------------------GSVMA-ACEE-----------------------------------------------------CRALVQALNKVTACFRQLVMSIGGSSDSLNLREELR MP-------------------LQNVKVADEGSVNFQKVKEE-----------------------------------------------------CITAVESLHKVVACYRHLALTIGGSSDSIHLRDELR MP-------------------IQNNKVGNEACINSLKVKEE-----------------------------------------------------CSTVVEALNKVVACYRHLAVSVGSSSDSKRLRDELR MP-------------------IQNIKVASEGSVNSQKTKEE-----------------------------------------------------CRALVEALNKVIACYRHLATCVGSSSDCCNLRDELR MP-------------------VSNNKIANDGNADSIKSREE-----------------------------------------------------AKALVEALIKVVACYRHLAASVGGSTDSSNLRDELR MP-------------------LSNNKVADDGS--STQPLQE-----------------------------------------------------GKAMVDSLIKVVACYRHLASCVGGCTDSSSLRRDLR MT-------------------LCNSKVADRGGDGSQQPLEE-----------------------------------------------------AKTLVDTLAKVVACYRQLSSCVGGSSDSVRLRGELR MP-------------------IQNSKVADEGTANSAKARDD-----------------------------------------------------CRSLVDSLNKVTGCYKHLAISVGGSSDCTNLRDELR MP-------------------IQSSKVADEGTANSARERDE-----------------------------------------------------CRALVDSLNKVTGCYRHLATGVGGSADCSNLRDELR MP-------------------IQNIKVADEGTVGSAKARDD-----------------------------------------------------CRALVDSLNKVTGCYRHLAIGIGGSADSAGLRDELR MP-------------------IQNIKVADEGAAGSAKARDD-----------------------------------------------------CRALVDSLNKVTGCYRHLAIGVGGSADSVGLRDELR MP-------------------IHSTKVADEGTAKSERARGE-----------------------------------------------------CKALVQSLNKVIGCYKHLAVNLGGSSDSTNLRDELR MQ---------------------RQ-PG-PREPE-DAATAE-----------------------------------------------------SRAALDALCRFAAGYRHLAMGVGSSADGPRLRLALE MR---------------------GGGAG-ERAREAREAVAE-----------------------------------------------------CRAAYEALNRFTASYRQLVLGVGSSADGRRLRRALE MR---------------------GAGPG-ERAREAREAVAE-----------------------------------------------------CRAAYEALNRFTASYRQLVLGVGSSADGRRLRRALE MR---------------------GAGPG-ERAREAREAVAE-----------------------------------------------------CRAAYEALNRFTASYRQLVLGVGSSADGRRLRRALE MA------------------------PG-RGAASGCGARGV-----------------------------------------------------CVATQAALCKAAAGHRQLVLQLGGSADGPQLREERR MA------------------------PGRRDACRGRGAVGT-----------------------------------------------------CATAQVALCKATAGHRQLVLQLGGSSDSPQLREERR MS---------------------HA----PDSHSVGEVRGA-----------------------------------------------------CAATHTSLSKVIVWHNQLALQLGGTSDSPQLRDELQ MA--------------------------------SRAPVGE-----------------------------------------------------CAAAHAALGRVTARHRQLALLLGSAADCAQLREELQ MP--------------------LLRSRSVDDAQNIGELVKE-----------------------------------------------------TKKAQALLSKILSCFRHLVLCIGGTSDSAKLREELE MAAPEVSSAGPNMLHPNSRTLTLRGKRGHAAEDSSQGAVSD-----------------------------------------------------CKKMHSSLNKVTACYRQLVLCVGGTSDCTRLREELE MSAPEVASPAPPHLHP-SRTLTLRGIRGHATESSGQGTVSE-----------------------------------------------------CKKVHISLNKVTACYRQLVLCVGGTSDCTRLREELE MS---------------------PWRRSVGELPAAQGSVAE-----------------------------------------------------CKKAQASLTRVTACFQQLAMCVGGSTDCSRLREELE MN---------------------RWQRSVVEIQTRKRQVNE-----------------------------------------------------CERAQVALSKVTACFQQMSTSLGSNVDGSFLREEME MN---------------------RWRKSVGEIQARRRAVAE-----------------------------------------------------CTRAQVAFSRVTACFQQLATCVGSSSDCSRLREELE MH-------------------LTIFPE--------------------------------------------------------------------RMLVREFNSATAYYRNLIITLGGSHDSESLRDEIR MG-------------------TWIFLLGLLGRGTPLQKRASEFVKPSSAIHSSGMNALHFGKNMIRRHNSCNRNHSDIQSDPETDEIEDCGMPLCTKLVKDFNVETARYHDLTLTLGGSSDSEYLRDELK ---------------------------------------------------------------------------------------------------------------MKKTLHVPDISEYLRDELK 1.......10........20........30........40........50........60........70........80........90.......100.......110.......120.......130

41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 41 40 41 41 41 41 48 43 48 47 58 58 58 58 56 58 58 58 58 58 58 53 55 55 55 52 53 52 45 57 77 76 56 56 56 43 111 19

R9AP_Human_NP-997274 R9AP_Cattle_NP-001159988 R9AP_Degu_XP-004636749 R9AP_Opossum_XP-007474849 R9AP_Tasmanian_devil_XP-003757239 R9AP_Koala_XP-020840579 R9AP_Ground-tit_XP-014110984 R9AP_Quail_XP-015729517 R9AP_Golden_eagle_XP-011572427 R9AP_Turtle_XP-007058713 R9AP_Alligator_XP-014451063 R9AP_Garter_snake_XP-013923971 R9AP_Gecko_XP-015270350 R9AP_Anole_XP-016852891 R9AP_Xenopus_XP-004913611 R9AP_Nanorana_XP-018425514 R9AP_Coelacanth_XP-006005212 R9AP_Florida_gar_28835-2-1 R9AP_Bowfin_1668-1-1 R9AP_Zebrafish_XP-005169975 R9AP_Asian_bonytongue_XP-018594265 R9AP_Reef_shark_40575-1-1 R9AP_Bamboo_shark_37642-1-1 R9AP_Bluespot_ray_25945-2-2 R9AP_Western_ray_30726-1-1 R9AP_Elephant_shark_XP-007887765 R9AP_M.mordax_41679-5-1 R9AP_G.australis_56404-2-1 R9AP_L.camtschaticum_JL14914 R9AP_E.cirrhatus_138946-5-1 R9AP-B_Xenopus_XP-002941417 R9AP-B_Nanorana_XP-018419161 R9AP-B-Partial_Coelacanth_XP-006013654 R9AP-B_Florida_gar_25574-1-1 R9AP-B_Zebrafish_NP-001314844 R9AP-B_Asian_bonytongue_XP-018613071 R9AP-B_Reef_shark_612-1-1 R9AP-B_Bamboo_shark_45543-9-1 R9AP-B_Bluespot_ray_37118-18-1 R9AP-B_Western_ray_39739-1-1 R9AP-B_Elephant_shark_XP-007902235 R9AP-like_Degu_XP-004636181 R9AP-like_Opossum_XP-007475029 R9AP-like_Tasmanian_devil_XP-003759057 R9AP-like_Koala_XP-020821390 R9AP-like_Quail_XP-015737481 R9AP-like_Ground-tit_XP-005524972 R9AP-like_Turtle_XP-007067829 R9AP-like_Alligator_XP-014450013 R9AP-like_Coelacanth_XP-005986755 R9AP-like_Xenopus_NP-001011277 R9AP-like_Nanorana_XP-018419805 R9AP-like_Spotted_gar_XP-006639786 R9AP-like_Zebrafish_NP-001018364 R9AP-like_Asian_bonytongue_XP-018611369 R9AP_S.kowalevskii_XP-006814063 R9AP_S.purpuratus_XP-011683108 R9AP_S.purpuratus_XP-011666321

: * : : * * :: : .: . . : :* KTRQKAQELAVSTCARLTAVLRDRGL-A--ADERAEFERLWVAFSGCLDLLEADMRRALELGAAFPL--HAPRRPLVRTGVAGA---SSGVAARALSTRSL-----RLE--AEGDFDVADLRELEREVLQ KTRQKAQELAVAIRLRLTAPLRDRSL-G--AEERAEFERLWVAFSGCLDLLEADMRRALALSTEFPL--QEPRRPLVRTGVEGG---ATGVAARALSVRSL-----RHE--AHRDFDVEDLHQLEREILQ KTRQRALELAVTTGARLTVALRDKGL-T--TEERAEFERLWVAFSGCLDLLEADMQRALALGAAFPL--HAPRRPLVRTGVAGS---CSGVAARALSARSL-----RHE--AERDFDRADLQELEQEVLQ QTRQKAHELAVANRNKLTAALKDKDV-S--KEDKAEYERLWVIFSTCLEILETDMKRALELGLEFPL--NMPKKHLIQTGMSGG---TTGVAARAMSVQNI-----KYE--AEPNIDEVDLKDLEQEIHK QTRQKAHDLAVANRNNLTAALKDKTV-S--KEDKAEFERLWVIFSTCLEILETDMKRALELGLEFPL--NVPKKHLIQTGLSGG---TAGVAARAMSVQNM-----KYE--AEHNIDEVDLKDLEQEIHK QTRQKAHDLAVANRNKLTAALKDKNV-S--KEDKAEFERLWVIFSTCLEILETDMKRALELGLEFPL--NVPKKHLIQTGMSGG---TAGVAARAMSVQNM-----KYE--ADRNIDEVDLKDLEQEIHK KTRQKAQELAVANRNKLTTVLKDKTV-S--KEDKAEFERLWVIFSTCLEILEIDMRRALELGHEFPL--NVPKKHLIQTGMSGG---TSGVAARAMSVQNM-----KYE--AEHNIDVVDLKDLENEINQ KTRQKAQELAVANRNKLTAVLRDKTV-S--KEDKAEFERLWVIFSTCLEILEIDMRRALELGHEFPL--NVPKKHLIQTGMSGG---TSGVAARAMSVQNM-----KYE--AEHNIDVVDLKDLENEINQ KTRQKAQELAVANRNKLTTVLKDKTV-S--KEDKAEFERLWVIFSTCLEILEIDMRRALELGHEFPL--NVPKKHLIQTGMSGG---TSGVAARAMSVQNM-----KYE--AEHNIDVVDLKDLENEINQ KTRQKAQELAVANRNKLTAVLKDKTV-S--KEDKTEFERLWVIFSTCMEILEIDMKRALELGQEFPL--NVPKKHLIQTGMSGG---TSGVAARAMSVQNM-----KYE--AEHNIDVVDLKDLENEINQ KTRQKAQELAVANRNKLTGVLKDRTV-S--KEDKAEFERLWVIFSTCMEILEVDMRRALELGHEFPL--NVPKKHLIQTGMSGG---TSGVAARAMSVQNM-----KYE--AEHNIDVVDLKDLEKEINQ KTRQKAQELAGVTRTQLTAALRNKTL-A--KEERAEFERLWVIFSACLEILEGDMRRALQLGQAFPL--HVPKKPLIQTGLSGG---TSGVAARAMSVQNM-----KYN--AEANIDVVDLTDLEQEISQ KTRQKAQELAVANRNKLTAALRDKTV-S--KEDRAEFERLWVLFTSCMEILETDMKRALELGQEFPL--NVPKKHLMQTGMSGG---TSGVAARAMSVQNM-----KYD--ADHNIDVVDLKDLENEINQ KTRRKAQELAVAARTRLTTALRDKTT-G--KEERAELERLWVLFSTCLELLETDMRRALELGQEFPL--NVPKKPLIQTGLSGG---TSAVAARAMSAQKM-----KYE--AGHDIDAADLKDLENEINQ KTRQKAQELAVANRNKLTSTLKDHRL-T--KEDKAEFERLWVIFTTCMDILETDMRRALVLGQEFPL--NIPKKHLIQTGMSGG---TSGVAARAMSVQNM-----RYD--AEHNIDVMDLKDLEDEINL KTRRKAQELAVANRNKLTGTLKDASL-V--KEDKAEFERLWVIFSTCMDILETDMRRALELGQEFPL--NIPKKHLIQTGMSGG---TTGVAARAMSVQNM-----KYE--AEHNIDIMDLKDLENEINQ KTRQKAQDLAVANRNKLTCILKDKTI-S--NEDRAEFERLWVIFSTCMEILEIDMKRALELGQEFPL--KVPRKHLIQTGMTGG---TSAVAARAMSVQNM-----KYE--DDNSIDIVDLKDLENEINQ RTRKKAQDLAVANRNKLTALLKDKTI-N--KEDRAEYERLWVIFSTSMELLELDMKKALEIGQEFPL--HVPKRHLIQTGMCGS---TSGVAARAMSTQNM-----KYE--ADSNIDVVDLKELEHEISQ KTRKKAQDLAVANRNKLTTLLKDKTI-N--KEDRAEYERLWVIFSTSMEFLEVDMKKALEFGQEFPL--NVPKRHLIQTGMCGS---TSAVAARAMSAQNM-----KYE--ADSSIDVVDLKELENEISQ KTRKKAQELAVANRTKLTALLKDKSI-S--KDDRAEYERLWVLFTSSMELLEVDMKRSLEIGQDFPL--KVPTRHLIQTGMTGS---TTTVAARAMSVQNM-----KYE--ADANIDTVDLRELETEIAQ KTRKKAQELAAANRTKLTALLKDKSI-S--KEDRSEYERLWVLFTSSMELLDVDMKRSLEIGQDFPL--QVPTRHLIQTGMSGS---TTTVAARAMSVQNM-----KYE--ADSNIDTADLKELEAEIAQ KTRQKAQVLAIANKIKLSTILKDRSI-S--KDDRAEFERLWVIFSTCMEILENDMRRALDLGQEFPL--NVPTKHLIQTGMIGR---TSAVAARAMSVQNM-----KYD--ECNDIDTVDLNELEQDINE KTRQKAQNLAIANKAKLTTILKDKTI-S--KDDRAEFERLWVVFSTCMEILEIDMRKALDLGQEFPL--NLPTKHLIQTGMIGG---TSAVAARAMSVQNM-----KYE--EGNNIDTVDLNDLEQDINE KTRRKAQVLAVANRNKLITILKDKTI-S--KNDRAEFERLWVLFSTCMEVLEVDMRRALELGQEFPL--NVPTKHLIQTGMIGG---TSAVAARAMNIQNM-----NYE--EGNNIDTVDLNDLEQEIYE KTRRKAQVLAVANKNKLITILKDKTI-S--KDDRAEFERLWVLFSTCMEVLEIDMRRALELGQEFPL--NVPTKHLIQTGMIGG---TTAVAARAMNIQNMNYEEGNYE--EGNNIDTVDLNDLEQEINE ETRQKARVLAVANRNRLTAALKDRAL-G--REDRAEFERLWVVFSTCVELLEVDMRRALELGHDFPL--HVPRRHLIQSGLSGG---TSAVAARAMSVRDM-----RYG--GGSNIDTADLSDLEQEIDE LTRERAHGIAASTRAALTRLLRDKEV-T--GERRAELERLWVSFSCCLELLEGDMRRALEISSQFPV--GGAR---VRTGVSGT---TAAVASRALSAKNV-----RSRDGAADEGGPEDAQALEKDVQE RTRERAHAVADSARVGLTRLLRDKAV-I--GERRADLERLWVSFSCCLELLEGDMRRALDVSGQFPV--GAAR---VRTGMTGT---TAAVASRALSAKDV-----RSREGAPAPGGPDDTQALEKDVQE CTRERAHGVADATRVALTLLLRDKAV-V--GERRAELERLWVSFSCCLELLEGDMRRALEISGQFPV--GAAR---VRTGMSGT---TVAIASRALSAKDV----------------------------Q RTRRRARELVGTIRGRLTSALRDGGAPM--RERRAEFERLWVAFASCLEQLEADMRRALELARIFPL--NMPRRALVQTGMSGG---TSGLAARALSTHSL-----RHCD-ASEHSEETDLLALQKDVIE RTRERAQELAVCNRNKLTTALRDKKL-S--KKEREELERLWVEFSCCLELFHNDMCKVYELGLAVPH--SATNKPAIQTGSTGD---TSAIASRALNVQNI---NYSD---SPANKASREYKEIEEEILK RTRERAQELAIGNRNKLTAALRDKQL-S--KEDRMELERLWVQFSSGLELFHNDMCKVYELGLSVPL--SANNQPAIQTGATGS---TSAIASRALSVQNI---TYESP--SSNNKERLEHNELENEILK KTRERAQELAVSNRNKLTAVLRDKSI-S--KEDRSDMECLWVIFSSALELFQTDMCKVFEIGQIFTL--SVTDVPVIQTGIT-----------------------------------------------RTREKAQELAVKNRSKLTASLRDKSL-P--KEERAEMERLWVVFSSCLELFHSDMCKVFEMGQTFSL--SSKSDPFVQTGMSGG---TSDVAARALSLQTL---THDE---NLPSIDRLEQNDLEAEIAK QTRERAQTLALSCRNHLTTRLRDKTL-P--EADRKEAEWLWVSFSSCLELLHADMSKVFAMAKHFSL--A-NNSTMVQTGIQGG---ATEVAARALSLPDL---QEAGAGTQTSSVEGQEQSQLEQEIAQ QTRERAQQLALTGRQLLTARLRDKAL-P--REERLEMELLWVAFSSSLEIFHADMCKVCSLGQNFPL--SSRTSALVQTGVQGG---MAEVTARALSLPEL---NRDE---PAASVERLEQGDLEVETTR KTREKTQDLAMVIRNKLTAVLRDKNL-S--KEERAEMERLWVIFSSNLETFQMDMCKVLELGQGFPL--SGTEKHLIQTGMSGG---TSEVAARALSVQNI---VHDS----TQNVENLELDELEEQIEK RTREKSQELAGLLRNKLTAALRDKAL-S--KEERAEMERLWVIFSSNLESFQVDMCKVLELGQGFPL--SGTEKPLIQTGMSGG---TSEVAARALSVQNL---AQDT----ALNGERLELQELQEHIDK KTREKTQDLAVTIRNKLTAVLRDKNL-G--KEERAEMERLWVIFCSSLELFQVDMCKVLELGQGFAL--AGTEKPLIQTGMSGG---ASEVTARALSVQNI---VHDP----APDVPSLEQRDLQEQIEK KTREKTQDLALVIRNKLTAVLRDKNL-G--KEERAEMERLWVIFCTSLELFQVDMCKVLELGQGFVL--AGTETPLIQTGMSGG---TSEVAARALSVQNI---VHDT----ALDVSSLEQRELQEQIEK RTRERAQELAAAIRTNLTAVLRDTEL-S--KEERPEMERLWVLFSSNLEVFHIDMCKVLDLGQSFPL--SGAERPLIQTGMCGD---TSEVTARALSVQNL---VHQT----SPNSERLQLQELEEQIGE EGGRRGLELSQGLHDQLLAELTQLGV-K--AETRAELERIWVLFVSTLDIFLQDLGRAHRLSQLFPVPAAAAGTQLVSTGVSSQ---GLKG----WSLRLA---STK-----AAQGAG----ELGQWVQQ ESGRRGFELSLGLRNQLLAEFTEPGT-RQ-EEVKMELERIWVLCLSALEIFLQDLGRAHDLSQLFPMPSPAPGIQLVNTGVTSF---GPKG----WSLRRP---SGK-----ASERAGELPEELAQWGQR ESGRRGFELSLGLRNQLLAELTEPGG-RQEEEVKMELERIWVLCLSALEIFLQDLGRAHDLSRIFPLPSPTSGTQLVNTGVISF---GPKG----WSLRRP---SGK-----TSERAGELPEELAQWGQR DSGRRGFELSLGLRNQLLAELTEPGG-RQEEEVKMELERIWVLCLSALEIFLQDLGRAHDLSQLFPLPSLAPETQLVNTGVTSF---GPKG----WSLRRP---SGK-----ASERARELPEELAQWGQR RRSVEVRELSVGLQSALLAWLQQA-V-G--PQEQRELERLWVLFLSALELFLQDLCCAHHLCCTFSP--GMGGVTPLRTGLGR------------W----------RGG---GPELPPTPP-CLEEEMEQ RRSAEACELSMGLRRVLLEGLRQAPV-S--PREQQELERLWVLFLSVLELFLQDLYRAQHLCQLFSV--KGGGIAPLHTGLGG------------W--GLP---SRRGG---GPTQPPPTQ-CLEEEIEQ ERSKEAHELSKGLRNMLLAGLQQPLA-S--PEERQELERLWVLSLSALELFHQDLCRAHHLCQLFPL--QGCGVQLLSTGVTGK---TTDGGHKPW--RRP---SRKGTR--STEQLGAAP-SLEEQIEH ERGKEAQELGTGLRQQLEAGLRPV-P-G--PEEQLELERLWVQLLSALELFQQDLRRAHHLSQLFPL--QGRGPQPLRTGIAGR---GGAGAARSW--RRA---SRKGGRILGAEQAGAGP-GLEERVAQ VTRKKAYILSTGLKSKLTMLLANKNL-G--SEHQSELERMWVLFLSCMEQFQQDLGKVLHFSQMFPLNVRGKH--LVNTGMTGK---TSGIAARVLNVQI-----------NSEVAENADSLDLKQHIDF ESRKKAFDLSTDLSNTLMVLLMNEGV-S--QEDRVELERIWVLFLSTLEFFQQDLCKAHHLCQLFPLHGRRKR--LVNTGVIGK---TSEVAYRARNIKSP---SSSRMHENQQRTERPCSPDLAGQIEH ESRKKAFEMSTDLRNTLMVLLMEQEV-T--RKDRVELERTWVLFLSTLELFQQDLCKAHHLCQLFPLHGRRKR--LVNTGVIGK---TSEVAYRARNIKSP----STRVKENPQRGERPCSPDLAGQIEH DTRLKAHHICTGLQGRLTALLTGAEL-G--PEEREEAERLWVLFLSGLDLFLQDLHKTAALQALFPLSQPRDRRALLHTGAAGR---TAEVAARAALVQAP---------WGQGEKEAEPPPDLGAHIAQ ETRTVAHKICSGLHRRLLSLLMEIDQ-G--QEDKEQVERLWVIFLSSLENFQQDLKKVKVLQEIFPLTQRKDRQALVITGLAGG---TSEVAARAAMVQTP---------WLS--VEVTVSPDLKTHIEE DTRALAHNLCMGLQKRLTALLTEGDL-V--QEDRERVERLWVLFLSGLEIFQQDLHKVVLLQDFFPLKQPRDRRMLVNTGGTSEGSSSGQVVARVAGVPIP---------WPK--EEAGPCPDLGTHIIQ KVRRKAHDLAKHNKYRLMPHLRNDFS-V--KTDRKDYERLWNAFTSCLEVFENQMRKSLELERTFPI--HAGPHVFINTGIVEP---MP-LELSPLSVEHL---DVPSI--DKRVLEREELKLLERDLND RTRKNAFHLAKKNMRKLLPHLKDKFD-F--KTERSEYERLWNMFSACLEVFEGHMRKSLELVKSFPI--HAGNKVLINTGVMEP---LG-VDNIPVNVENL---DIPCV--DKMVLEREDTRLLERDILE RTRKNAFHLAKKNMRKLLPHLKDKFD-F--KTERSEYERLWNMFSACLEVFEGHMRKSLELVKSFPI--HAGNKVLINTGVMEP---LG-VDNIPVNVENL---DIPCV--DKMVLEREDTRLLERDILE .......140.......150.......160.......170.......180.......190.......200.......210.......220.......230.......240.......250.......260

156 156 156 156 156 156 156 156 156 156 156 156 156 156 156 156 156 156 156 156 156 155 156 156 161 156 162 157 139 164 174 175 135 174 174 174 173 173 173 173 173 161 168 169 169 150 157 166 160 168 196 194 171 169 172 159 227 135

R9AP_Human_NP-997274 R9AP_Cattle_NP-001159988 R9AP_Degu_XP-004636749 R9AP_Opossum_XP-007474849 R9AP_Tasmanian_devil_XP-003757239 R9AP_Koala_XP-020840579 R9AP_Ground-tit_XP-014110984 R9AP_Quail_XP-015729517 R9AP_Golden_eagle_XP-011572427 R9AP_Turtle_XP-007058713 R9AP_Alligator_XP-014451063 R9AP_Garter_snake_XP-013923971 R9AP_Gecko_XP-015270350 R9AP_Anole_XP-016852891 R9AP_Xenopus_XP-004913611 R9AP_Nanorana_XP-018425514 R9AP_Coelacanth_XP-006005212 R9AP_Florida_gar_28835-2-1 R9AP_Bowfin_1668-1-1 R9AP_Zebrafish_XP-005169975 R9AP_Asian_bonytongue_XP-018594265 R9AP_Reef_shark_40575-1-1 R9AP_Bamboo_shark_37642-1-1 R9AP_Bluespot_ray_25945-2-2 R9AP_Western_ray_30726-1-1 R9AP_Elephant_shark_XP-007887765 R9AP_M.mordax_41679-5-1 R9AP_G.australis_56404-2-1 R9AP_L.camtschaticum_JL14914 R9AP_E.cirrhatus_138946-5-1 R9AP-B_Xenopus_XP-002941417 R9AP-B_Nanorana_XP-018419161 R9AP-B-Partial_Coelacanth_XP-006013654 R9AP-B_Florida_gar_25574-1-1 R9AP-B_Zebrafish_NP-001314844 R9AP-B_Asian_bonytongue_XP-018613071 R9AP-B_Reef_shark_612-1-1 R9AP-B_Bamboo_shark_45543-9-1 R9AP-B_Bluespot_ray_37118-18-1 R9AP-B_Western_ray_39739-1-1 R9AP-B_Elephant_shark_XP-007902235 R9AP-like_Degu_XP-004636181 R9AP-like_Opossum_XP-007475029 R9AP-like_Tasmanian_devil_XP-003759057 R9AP-like_Koala_XP-020821390 R9AP-like_Quail_XP-015737481 R9AP-like_Ground-tit_XP-005524972 R9AP-like_Turtle_XP-007067829 R9AP-like_Alligator_XP-014450013 R9AP-like_Coelacanth_XP-005986755 R9AP-like_Xenopus_NP-001011277 R9AP-like_Nanorana_XP-018419805 R9AP-like_Spotted_gar_XP-006639786 R9AP-like_Zebrafish_NP-001018364 R9AP-like_Asian_bonytongue_XP-018611369 R9AP_S.kowalevskii_XP-006814063 R9AP_S.purpuratus_XP-011683108 R9AP_S.purpuratus_XP-011666321

VGEMIDNMEMKVNVPRWTVQARQAAGAELLSTVS----AGPSS--------V--VSL--QER-----------GGGCD----PR---------------KALAAIL-----------------------VGEMIQDMEMKVNVPRWTVQARQAAGAELLSSAS----AGVSS--------VGGVSV--EQR-----------TGPCD----LS---------------KAKAATI-----------------------VGKMINEMEMKVNVPRWTVQARQTAGAELLSSAS----AGASS--------A--VSV--PER-----------TGPCD----LS---------------KALAATV-----------------------VGEMMYEMEMKVNVPQWTVEAKQDPGAELKSTTS----AGASS--------GGFVSV--EER-----------KSVCD----IS---------------KLLAAIV-----------------------VGEMMYEMEMKVNVPQWTVEAKQDPGAELKSTIS----TGASS--------GGFVSV--EER-----------KGFCD----IS---------------KLLAAII-----------------------VGEMMYEMEMKVNVPQWTVEAKQDPGAELKSTTS----AGASS--------GGFVSV--EER-----------KGFCD----IS---------------KLLAAIV-----------------------VGEMMYEMEMKVNVPQWTVEAKQDPGAELKSTIS----VGASS--------IGMISV-EEN------------KSFCD----IS---------------KVLAGII-----------------------VGEMMYEMEMKVNVPQWTVEAKQDPGAELKSTIS----VGASS--------IGMISV-EEN------------KSFCD----IS---------------KVLAGIV-----------------------VGEMMYEMEMKVSVPQWTVEAKQDPGAELKSTIS----VGASS--------IGMISV-EEN------------KSFCD----IS---------------KVLAGII-----------------------VGEMMYEMEMKVNVPQWTVEAKQDPGAELKSTIS----VGASS--------IGMISV-EEN------------KSFCD----IS---------------KVLAGII-----------------------VGEMMYEMEMKVNVPHWTVEAKQDPGAELKSTVS----MGASS--------MGMISV-EEN------------KSFCD----IS---------------KVLAGVI-----------------------VGEMIYEMEMKVNVPRWTVEAKQDPGTELNSNIS----GGAAS--------LGAVSG--VEN-----------KTLCD----PS---------------RILAAII-----------------------VGEMMYEMEMKVNVPQWTVEAKQDPGAELNSAIS----VAASS--------IGMIEV--ES------------KSYCN----AR---------------KAVAGSV-----------------------VGEMRYEMEMKVSVPQWTVEAKQDPGAELHSTVS----AGASS--------LGLISA--EEP-----------KAFCD----VS---------------RVLAGII-----------------------MDEMMYEMEMKVNVPQWTVEAKQDPGAELKSTMS----AGASP---------GIISL--DDH-----------KSICD----LN---------------KVLAGVV-----------------------VGEMMYEMEMKVSVPQWTVEAKQNPGAELKLAAA----TGSTS--------PGTVST--EDR-----------KAICD----LS---------------KLLAAMI-----------------------VEEMLYEMEMKVNVPHWTVEAKQDPGSELKSTIS----VGASS--------VGALSVD--GS-----------KSFCD----PS---------------KALAGVI-----------------------VGEMMEEMEMKVNVAHWMVEAKQDPGAELKSTIS----VGASS--------IGVISVNEEN------------KSPCD----PT---------------KILAGVV-----------------------VGEMMEEMEMKVNVAHWTVEAKQDPGAELKSTIS----VGASS--------IGVISINEES------------KSGCE----PT---------------KVLAGVI-----------------------VDQMMEEMEMKVQVAPWAVEAKQEAGAELKSTVS----VGNSS--------VGVISICEEEP-----------KETVEGENGTM---------------KIFAGII-----------------------VVQMMEEMEMKVQVAPWAVEAKQEAGAELKSTVS----VGNSS--------VGVISICEEEP-----------KE-MDSEAGLK---------------KVFAGII-----------------------VKEMIYEMEMKVNVLQWTVEAKQAAGPE----RP----AGASS--------LGMISVHDDGN-----------KKLCD----TS---------------KVFAGAM-----------------------IGEMMYEMEMKVNVLQWTVEAKQELGTELKSAAS----AGASS--------FGMRPMNEGTD-----------KRLCD----TN---------------KVFAGAM-----------------------VGEMIHEMEMKVNVLQWTVEAKQGPGSELKSTAS----AGVSS--------LAAMSVSGDG------------DKLCH----TS---------------KVFAGAM-----------------------VGEMIYEMEMKVNVLQWTVEAKQGPGSELKSTAS----AGASS--------FAVMSVNGGGN-----------DKLCD----IS---------------KVFAGAM-----------------------VGDMLYQMEMKVGVPHWTVQTTRRRPQRNGGWNA----GRTDG--------RTARPER--------------------------------------------ASSR-----------------------LGTMLNEMENKVNVPRWSVVAAHEPGAELHSTAS----G--SS--------VAMLTEEGARG-----------DPCCY----PG---------------RRLVAAF-----------------------LGTMLNEMENKVNVPRWSVVAAQEPGAELHSTAS----G--SS--------VAMLTEEGARG-----------DPCCD----PG---------------RRLVAAF-----------------------LGTMLNEMENKVNVPRWSVVAAREPGAELHSTAS----G--SS--------VAMLTEEGARG-----------EPCCD----PG---------------RRLVAAF-----------------------VGEIIAEMENKVNVPRWSIIAIEEPGAELRSTAI----GGGTS--------TGMLNV-GARR-----------RRTCR----LS---------------RRLSALL-----------------------VDNMITDMEMKVNVLRWTVEANTR------INDELKSTHDSSS--------VVLLSEEESNS---------KG--CCS----DG---------------QLIVSLL-----------------------VDQMITDMELRVNVLRWTVEATAN------MNDE-RDNNDVSS--------TALLSVDEKES---------HN--CCN----GC---------------GLL------------------------------------------------------------------------------------------------------------------------------------------------------------VDKMIDNMEMKVNVLRWTVEAKSP------LYADPLST-ETSS--------VALLSLDEEDG---------GR--CCD----RS---------------QFFVSVV-----------------------VDRTLDDMEMKVNVLRWTVEAVGP------QYTDPVST-DSTS--------LALLSMDEEAA---------QS--FCS----RS---------------QMFAAMM-----------------------VDQMIEDMEQKVNVLRWTVEARGP------RYAEPLSAGDGSS--------LALLSADAEDP---------GR--RRE----RS---------------QLLVVVV-----------------------VDRMMENMEMKVNVLRWTVEAKGD------AYHSVISN-DASS--------VALLCAEEEMR---------SN-QCCD----RE---------------TCFMSML-----------------------VDEMMENMEMKVNVLRWTVEAKGD------AYYSVLST-DASS--------MALLSEEEEMR---------RHGRCCD----RE---------------KCFMSVL-----------------------VDRMVENMEMKVNVLRWTVEAKGD------AYHSVLSN-DTSS--------VALLSVDEEVR---------GW-WCCH----RS---------------KCLTSML-----------------------VDRMVENMEMKVNVLRWTVEAKGD------AYHSVLSN-DASS--------VALLSVDEEVR---------GW-WCCD----RS---------------KCLTSML-----------------------VDRMAEDMEMKVNVLRWTVEAKGE------EIHSVVSN-DFSS--------TALLPSEDEVR---------RR-ECCD----RE---------------KWLVVMA-----------------------LAKLQLEMEKEVNVPPWGVEPSMGDLV-----------SEAAS--------LFHHYREEARA-------------RCQ----ANPY---------------CRSKG-----------------------LEEMLLEMETKVNVPVWSVEATVGGPG-----------AEEPS--------PEGSARRSPPG-------------CCA----QAPPGTTFGLQLTNFPSCLWRIKQ-----------------------LEEMLLEMETKVNVPVWSVEATVGDPE-----------ALEHS--------PESSAGRSPRG-------------CCA----RGPD----------------RLQS-----------------------LEEMLVEMETKVNVPVWSVEATVDSPG-----------AEEPS--------LEGSAGRSPRG-------------CCA----RAPL----------------RLQS-----------------------VRAMMVEMESTANIPLWTVESMHVVGP-----------EE--G--------TEPTSHRPGPG----------TGSCCR----VL---------------------------------------------VRATLAEMESGANIPPWTVEATETTQP-----------AETSG--------TTERAAWGGPC----------AGHCCG----VL---------------------------------------------VGAMLLEMETRVNVPVWTVEATEETGPETSLAFE----AEGSC--------SERQAAEVAGGLGCCGQCQDWPALCCT----LS---------------------------------------------VGAMLTEMETSLNIPVWTVEATEAAWAERSSAAE----AE-----------AEGVGGEMPGGM-------EAPGHCCT----VS---------------------------------------------IGKTLQEMELKANIPIWSVEANEEAWAEVTSAVAL---EDASS--------NDVLTMDESTN------R-----HCCY----R----------------GCWL--------------------------MERMLHDMQMKVSIPIWTVEATEEAWAEVASTCDL---DECSD--------NEILAGEDITS------R-----GCCA----H----------------GQSLPGP-----------------------VERMLHDMQMKVSIPIWTIEATEEAWAEVSSTCDL---DDCSD--------NEILAGEDISS------R-----GCCA----Q----------------RQSLARP-----------------------LERMAQDLLQRVSVPIWSVEATQEAWAEGSLA-ELDPDDRVTS--------EELLTVEELSQ------GAERQAGCCR----YP---------------GCRLGWI-----------------------IDVLLEEMLQRVNVPLWSVEPTQEAWVEGSSTPGVGQEEDESL--------EEMMEVEVVSQ------N--KTSGCCH----HH---------------NCKVGCL-----------------------LETMAQEMLRKVNVPFWSVEATQEAWAEGCDN----EQDDEEV--------VEEMSVETVTT------EGGRLAGCCH----YP---------------GCRI--------------------------LREMLLEMHRSIDIKPWTIEAVHDSKVDINKSQSSMSE-VESSADVSHVTFTAYHNDTDKTH---------HK---------KT---------------SEKIVVFRGPGCCKYGKTEWRNQVLEIPPVL LRNLMSEMHRCVDIKPWTIQPPSDYKLDTKSQTSLNSE-TASS--------TGFDPQYDAEP---------DA---------SV---------------PFCGCRS-----------------------LRNLMSEMHRCVDIKPWTIQPPSDYKLDTKSQTSLNSE-TASS--------TGFDPQYDARQ---------RR---------KC---------------AFLVSVA-----------------------.......270.......280.......290.......300.......310.......320.......330.......340.......350.......360.......370.......380.......390

216 218 216 218 218 218 218 218 218 218 218 218 217 218 217 218 218 219 219 224 223 215 220 219 225 206 224 219 201 227 236 232 135 235 235 236 235 236 235 235 235 216 238 223 223 199 208 234 218 229 260 258 243 240 238 255 291 199

R9AP_Human_NP-997274 R9AP_Cattle_NP-001159988 R9AP_Degu_XP-004636749 R9AP_Opossum_XP-007474849 R9AP_Tasmanian_devil_XP-003757239 R9AP_Koala_XP-020840579 R9AP_Ground-tit_XP-014110984 R9AP_Quail_XP-015729517 R9AP_Golden_eagle_XP-011572427 R9AP_Turtle_XP-007058713 R9AP_Alligator_XP-014451063 R9AP_Garter_snake_XP-013923971 R9AP_Gecko_XP-015270350 R9AP_Anole_XP-016852891 R9AP_Xenopus_XP-004913611 R9AP_Nanorana_XP-018425514 R9AP_Coelacanth_XP-006005212 R9AP_Florida_gar_28835-2-1 R9AP_Bowfin_1668-1-1 R9AP_Zebrafish_XP-005169975 R9AP_Asian_bonytongue_XP-018594265 R9AP_Reef_shark_40575-1-1 R9AP_Bamboo_shark_37642-1-1 R9AP_Bluespot_ray_25945-2-2 R9AP_Western_ray_30726-1-1 R9AP_Elephant_shark_XP-007887765 R9AP_M.mordax_41679-5-1 R9AP_G.australis_56404-2-1 R9AP_L.camtschaticum_JL14914 R9AP_E.cirrhatus_138946-5-1 R9AP-B_Xenopus_XP-002941417 R9AP-B_Nanorana_XP-018419161 R9AP-B-Partial_Coelacanth_XP-006013654 R9AP-B_Florida_gar_25574-1-1 R9AP-B_Zebrafish_NP-001314844 R9AP-B_Asian_bonytongue_XP-018613071 R9AP-B_Reef_shark_612-1-1 R9AP-B_Bamboo_shark_45543-9-1 R9AP-B_Bluespot_ray_37118-18-1 R9AP-B_Western_ray_39739-1-1 R9AP-B_Elephant_shark_XP-007902235 R9AP-like_Degu_XP-004636181 R9AP-like_Opossum_XP-007475029 R9AP-like_Tasmanian_devil_XP-003759057 R9AP-like_Koala_XP-020821390 R9AP-like_Quail_XP-015737481 R9AP-like_Ground-tit_XP-005524972 R9AP-like_Turtle_XP-007067829 R9AP-like_Alligator_XP-014450013 R9AP-like_Coelacanth_XP-005986755 R9AP-like_Xenopus_NP-001011277 R9AP-like_Nanorana_XP-018419805 R9AP-like_Spotted_gar_XP-006639786 R9AP-like_Zebrafish_NP-001018364 R9AP-like_Asian_bonytongue_XP-018611369 R9AP_S.kowalevskii_XP-006814063 R9AP_S.purpuratus_XP-011683108 R9AP_S.purpuratus_XP-011666321

-----------FGAVLLAAVALAVCVAKLS-------------------------------------------------------------------------------FSAVLLAAVALAVCVAKLS-------------------------------------------------------------------------------FSAVLLAAVALALCVAKLS-------------------------------------------------------------------------------FSAVLIIAVILAVCVVKLS-------------------------------------------------------------------------------FSAVLIIAVILAVCVVKLS-------------------------------------------------------------------------------FSAVLIIAVILAVCVVKLS-------------------------------------------------------------------------------FTAVLLIAIVLAVCVVKLS-------------------------------------------------------------------------------FSAVLIIAIVLAVCVVKLS-------------------------------------------------------------------------------FSAVLIIAIVLAVCVVKLS-------------------------------------------------------------------------------FSAVLIIAIVLAVCVVKLS-------------------------------------------------------------------------------FSAVLIIAIVLAVCVVKLS-------------------------------------------------------------------------------FTAVLLIAIGLAVCVAKLS-------------------------------------------------------------------------------FSAVLLIAVVLAVCVVKLS-------------------------------------------------------------------------------FTAVLLVAIILAVCVAKLS-------------------------------------------------------------------------------FSAVLIIAIILAVCVVKLS-------------------------------------------------------------------------------FSAVLLVAVILAVCVVKLS-------------------------------------------------------------------------------FTAVLMIAIVLAVCVVKLS-------------------------------------------------------------------------------FTAVLFVAIVLALCVIKLS-------------------------------------------------------------------------------FTAVLLIAIILAFCVIKLS-------------------------------------------------------------------------------FTAVLLIAGILGYLVINL--------------------------------------------------------------------------------FTAIILMAGILAFCVIKLS-------------------------------------------------------------------------------FAAVLIFAIALAVCVVKFA-------------------------------------------------------------------------------FTAVLVFAIVLALCVVKFA-------------------------------------------------------------------------------FSAVLIIAIVLAFCIVKFA-------------------------------------------------------------------------------FSAVLIIAIVLAACIVKFA-------------------------------------------------------------------------------FP-------TRAPTRSRTA-------------------------------------------------------------------------------CGGALLLALVLAAAVVIFS-------------------------------------------------------------------------------CGGALLLAFVLAAAVVMFA-------------------------------------------------------------------------------CGGALLLALVLAAAVVMFA-------------------------------------------------------------------------------FTMIILLAVVLSVCVAKFA-------------------------------------------------------------------------------FCGTALVAITLYS----IL------------------------------------------------------------------------------------------------FL---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LCGIAMVAVVLSVCVVYLT-------------------------------------------------------------------------------LCGVAMFAVVLSVCVVFLV-------------------------------------------------------------------------------VSVAVAVATMLFVCIVYLV-------------------------------------------------------------------------------FCTVALIAVVLSVCVVNLA-------------------------------------------------------------------------------FCTVALIAVGLSVCVVNLS-------------------------------------------------------------------------------LCTAALAAVVLSVCAANIA-------------------------------------------------------------------------------LCTAALTAVVLSVCAANLA-------------------------------------------------------------------------------LCTAALVAVVLSVCVATFA---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HKNSF---------------------------------------------------------------------------------------------HCIVA---------------------------------------------------------------------------------------------HCIVA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ICVIS---------------------------------------------------------------------------------------------LCMVS---------------------------------------------------------------------------------------------LCMVS---------------------------------------------------------------------------------------------LCLVN---------------------------------------------------------------------------------------------LCLLS---------------------------------------------------------------------------------------------LCMLH----------------------------------------------------------------------------------LSGLRGAHFIDIKYLVEFKAVVPLAPFDLQVKLPITIGSVPLQQIHAQLDQRAASLGPGCATPELSAQQDDVPVPNIPPPTYEQVCGGGQVNDAEDNKL -----------V-------------------------------------------------------------------------------------------------LITLVAFAAVLIICLVLLEGK------------------------------------------------------------------.......400.......410.......420.......430.......440.......450.......460.......470.......480.........

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