Aug 27, 2013 - is dependent on autophagy, TLR4, and TLR9 signaling in liver .... microscope (Carl Zeiss, Oberkochen, Germany). Western blot analysis ..... 2062. 6. Watanabe, E., Muenzer, J. T., Hawkins, W. G., Davis, C. G.,. Dixon, D. J. ...
The FASEB Journal article fj.13-229476. Published online August 27, 2013.
The FASEB Journal • Research Communication
Experimental sepsis-induced mitochondrial biogenesis is dependent on autophagy, TLR4, and TLR9 signaling in liver Evie H. Carchman,*,§ Sean Whelan,* Patricia Loughran,*,§ Kevin Mollen,* Sladjana Stratamirovic,*,§ Sruti Shiva,†,‡ Matthew R. Rosengart,* and Brian S. Zuckerbraun*,‡,§ *Department of Surgery, †Department of Pharmacology, and ‡Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA; and §Veterans Affairs Pittsburgh Healthcare System, Pittsburgh, Pennsylvania, USA Organ injury in sepsis is initially characterized by dysfunction without cell death and structural damage, and thus with the ability to recover organ function. Adaptive metabolic responses to sepsis can prevent bioenergetic failure and death. These studies were aimed at investigating the influence of sepsis on mitochondrial homeostasis, focusing on removal of dysfunctional mitochondria and restitution of a healthy mitochondrial population. These data demonstrate decreased hepatic oxidative phosphorylation by 31 ⴞ 11% following murine cecal ligation and puncture (CLP) at 8 h and 34 ⴞ 9% following LPS treatment in vitro at 12 h (P
