Exposure to Cigarette Smoke Worsens Chronic Kidney Disease Induced ... increased cardiac and renal fibrosis in PNx-operated animals, resulting in 2.5- and ...
Exposure to Cigarette Smoke Worsens Chronic Kidney Disease Induced Cardiac Hypertrophy and Fibrosis C.A. Drummond1, D-A. Hernandez1, S.T. Haller1, J. Liu2, J.I. Shapiro2, C.J. Cooper1, J. Tian1 1University of Toledo, Department of Medicine, College of Medicine and Life Sciences, Toledo, Ohio; 2Marshall University, Joan C. Edwards School of Medicine, Huntington, WV Poster Session Title: Kidney in Hypertension Program Number: 965.4 Abstract Number: 1821 Poster Board Number: B211 A.
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Figure 1: Smoke Exposure protocol design. Abbreviations: PNx, 5/6th partial nephrectomy; Echo, echocardiographic evaluation of cardiac structure and function by echocardiography; BP, blood pressure measurement by tail cuff; BC, Blood collection; RF, renal function assessment; Sac, sacrifice.
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Tube containing air with smoke to Animal Holding Chamber
5L Animal Holding Chamber with holes in the lid
Tube containing room air flowing from pump
Tube containing air with smoke to Animal Holding Chamber Cigarette insertion opening
Figure 2: Smoke Exposure apparatus. Representative images of the combustible cigarette exposure apparatus used in this study. Panel A. wide angle view of complete assembled system; panel B, detailed view of air-flow; panel C, detailed view of the top of the 3L dilution canister showing air-flow; panel D, detailed view showing animal holding container.
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Figure 7: Smoking and PNx both independently and when combined significantly increased Collagen 1, CD40 receptor and PAI-1 protein expression. Top Representative Western Blots from Renal tissue, bottom computer aided densitometry. Mean +/- SEM are shown from measurements in N=5 Sham A, 5 Sham S, 5 PNx A, and 5 PNx S animals. *, indicates mean is significantly different than Sham A (p
