Extraction and residual antinutritional components in protein fractions ...

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Email: [email protected] .... adjustment; this was kept and analysed for the determination of mass balance of ... oil-free meal at 10% moisture (Mailer et al.
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17 Australian Research Assembly on Brassicas (ARAB)

Wagga Wagga NSW August 2011

Extraction and residual antinutritional components in protein fractions of Sinapis alba and Brassica napus oil-free meals 1,2

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Siong H. Tan , Rodney J. Mailer , Christopher L. Blanchard and Samson O. Agboola 1 EH Graham Centre for Agricultural Innovation, Wagga Wagga, NSW 2650, Australia 2 School of Agricultural and Wine Sciences, Charles Sturt University, Private Bag 588, Wagga Wagga, NSW 2678, Australia 3 NSW Department of Primary Industries, Wagga Wagga, NSW 2650, Australia, 4 School of Biomedical Sciences, Charles Sturt University, Private Bag 588, Wagga Wagga, NSW 2678, Australia Email: [email protected]

ABSTRACT Proteins were sequentially extracted from Australian canola (Brassica napus) and mustard (Sinapis alba) meals and two industrial canola meal samples, according to Osborne method (OSB), based on their solubilities in water, 5% NaCl, 0.1 M NaOH and 70% ethanol. These extracts were then compared to the glutelin fraction obtained by direct alkaline extraction method (DIR) in terms of protein yield, recovery, and residual antinutritional components. The OSB method was found to be more effective for protein extraction which resulted in higher cumulative protein yield and recovery of water-soluble protein fractions, although the DIR method produced a glutelin fraction of higher protein content. Phenolic compounds were observed in all the extracted protein fractions although sinapine, the major phenolic compound in canola, was present only in the albumin fractions. The glucosinolate content of all extracts was below the detection limit of the method employed (