Henry A. Ross,3 Marie-Cecile. Jacobs,2 Jacques W. M. Lenders,2 Theo Thien,2 ...... MD, Angus. JA, Cox HS, Eisenhofer G. Responses to nitroprusside-induced.
LlN.
CHEM.
41/10,
1455-1460
(1995)
#{149} Automation
and Analytical
Techniques
Sensitive and Specific HPLC with Fluorometric Detection for Determination of Plasma Epinephrine and Norepinephrine Applied to Kinetic Studies in Humans F-lighly
lacques J. Willemsen,’ Henry A. Ross,3 Marie-Cecile Leon M. J. W. Swinkels, and Theo J. Benraad1’4
Jacobs,2
n HPLC separation method combined with fluorometric ietection was extended to enable simultaneous assess‘nent of plasma 3H-labeled and endogenous epinephrine E) and norepinephnne (NE). Forearm fractional extracion (FFE) of 3H-labeled E and NE and of endogenous E as measured in 40 healthy volunteers who were receivng a continuous infusion of 3H-labeled E and NE. Conentrations of arterial and venous E were 26.8 ± 1.95 mean ± SE) and 6.8 ± 0.75 ng/L, respectively. Arterial and venous NE and dopamine (DA) were also measured, Nith respective values of 140.7 ± 8.5 and 192.1 ± 15.1 or NE, and 13.1 ± 0.78 and 11.3 ± 0.70 ng/L for DA. The FFE of 3H-labeled E was 0.790 ± 0.016) than the sndogenous E (0.748 ± respectively; P
