ï¬xed in 100% methanol at â20 °C for 10 min., washed three times with. PBS, and ..... Claudius Vincenz, Arul Chinnaiyan, and Zhi Zheng for helpful discus- ... Wilson, K. P., Black, J. A. F., Thomson, J. A., Kim, E. E., Grifï¬th, J. P., Navia,. M. A. ...