Fecal microbial characterization of hospitalized patients with ...

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Medical Center, 3333 Burnet Ave, Cincinnati, OH, 45229, USA. Tzipi Braun and ..... Bartosch, S., Fite, A., Macfarlane, G. T. & McMurdo, M. E. Characterization of bacterial communities in feces from healthy elderly volunteers .... Frank, D. N. et al.
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Received: 8 December 2016 Accepted: 27 March 2017 Published: xx xx xxxx

Fecal microbial characterization of hospitalized patients with suspected infectious diarrhea shows significant dysbiosis Tzipi Braun1, Ayelet Di Segni1, Marina BenShoshan1, Roy Asaf1, James E. Squires2, Sarit Farage Barhom1, Efrat Glick Saar1, Karen Cesarkas1, Gill Smollan1, Batia Weiss1,3, Sharon Amit1, Nathan Keller1,3 & Yael Haberman1,4 Hospitalized patients are at increased risk for acquiring healthcare-associated infections (HAIs) and inadequate nutrition. The human intestinal microbiota plays vital functions in nutrient supply and protection from pathogens, yet characterization of the microbiota of hospitalized patients is lacking. We used 16S rRNA amplicon sequencing to characterize the global pattern of microbial composition of fecal samples from 196 hospitalized patients with suspected infectious diarrhea in comparison to healthy, non-hospitalized subjects (n = 881), and to traditional culture results. We show that hospitalized patients have a significant rise in α-diversity (richness within sample) from birth to