Supported by: The Netherlands Organisation for Health Research and De- ... influence of several luteal support protocols on miRNA profiles. DESIGN: ...
P-15 Tuesday, October 20, 2009 SPERM OXIDATIVE STRESS, DNA FRAGMENTATION AND CHROMOSOME Y MICRODELETIONS AS ORIGIN OF IDIOPATHIC RECURRENT SPONTANEOUS ABORTION. J. Bellver, M. Meseguer, L. Muriel, A. L. Garda, S. Garcia-Herrero, N. Garrido. Instituto Universitario IVI VAlencia, Valencia, Spain; IVI Vigo, Vigo, Pontevedra, Spain; IVI Murcia, Murcia, Spain. OBJECTIVE: To assess whether sperm oxidative DNA damage, sperm DNA fragmentation, and chromosome Y microdeletions are related to idiopathic recurrent spontaneous abortion, in a cases and controls study. DESIGN: The study was approved by the local Institutional Review Board. Three groups of thirty young men (< 40 years) were included prospectively: A- Fertile sperm donors; B – Infertile men with persistent oligozoospermia (1-5 mill/ml) without previous miscarriages; C- Men non severe (>5mill/ml) sperm alteration from couples with R 3 first trimester clinical spontaneous abortions, normal paternal karyotypes, and female partners < 38 years without acquired or inherited thrombophilia, normal uterus and no endocrine disturbance. Sperm and blood samples were collected in all the included patients. MATERIALS AND METHODS: Chromosome Y microdeletions (AZFa, AZFb, AZFc and AZFd regions) were analyzed in blood by multiplex PCR and sperm oxidative DNA damage and DNA fragmentation by OXIDNA assay and Sperm Chromatin Dispersion test kits. ANOVA was employed to compare DNA oxidation and fragmentation means among groups. Chi square Fisher’s exact tests were employed to compare microdeletion frequencies. p < 0.05 was considered statistically different. RESULTS: Differences were found in the % of sperm DNA fragmentation among groups: A 24.1 CI95% 20.9-27.2, B 46.0 CI95% 40.4-51.6, C 33.5% CI95% 28.3-38.7. Comparable levels of mean sperm with oxidized DNA, as well as oxidation levels were found. Regarding the % of stained cells A mean was 32.1 CI95% 24.6-39.66, B 24.0 CI95% 16.9-31.2, C 32.4 CI95% 24.6 40.2. Considering the mean oxidation levels (RFU), A mean was 87.25 CI95% 61.6, B 87.6 CI95% 63.7-113.3, C 68.0 CI95% 53.6-82.13. No Y microdeletions were found in any male. CONCLUSIONS: Sperm DNA quality features in terms of oxidation are not related with idiopathic recurrent abortion. Y chromosome microdeletions are not involved in the pathological process of recurrent pregnancy loss, but sperm DNA fragmentation does.
CONCLUSIONS: Overall quality of care in RM was poor. To improve quality of care in patients with RM, further research on implementation of the guidelines should focus on the aspects of care that reveal room for improvement. [1] McGlynn EA, Asch SM, et al. The quality of health care delivered to adults in the United States. NEJM 2003;348:2635-45. Supported by: The Netherlands Organisation for Health Research and Development (ZonMw) (Grant no. 94517005).
FEMALE REPRODUCTIVE ENDOCRINOLOGY P-17 Tuesday, October 20, 2009 STEROID SUPPLEMENTATION DURING LUTEAL PHASE INFLUENCES MICRORNA EXPRESSION PROFILES IN THE HUMAN ENDOMETRIUM AFTER CONTROLLED OVARIAN STIMULATION WITH GNRH ANTAGONIST PROTOCOL. Y. Zhao, J. Garcia, C. Cheadle, N. F. Vlahos. Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Lutherville, MD; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD; 2nd Department of Obstetrics and Gynecology, University of Athens Medical School, Athens, Greece. OBJECTIVE: Studies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. This study was designed to evaluate the expression of miRNAs during the luteal phase following controlled ovarian stimulation (COS) for IVF and the influence of several luteal support protocols on miRNA profiles. DESIGN: Endometrial biopsies were obtained from 9 oocyte donors during the luteal phase of stimulated cycles MATERIALS AND METHODS: Endometrial biopsies were obtained on the day of and 3 to 5 days after oocyte retrieval during COS cycles using a recombinant FSH/ganirelix acetate protocol. Study subjects were randomized into three groups: no luteal support, luteal support with micronized progesterone (P) and luteal support with progesterone plus 17-ß-estradiol (PþE). Total RNA was isolated and microarray analysis was performed using an Illumina miRNA expression panel. RESULTS: A total of 526 miRNAs were identified. Of these, 216 miRNAs were either significantly up- or down-regulated (p
