Figure S4 Gene expressions in transgenic mice. Expression of ... - PLOS

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No expression of EZR-ROS1 fusion protein was detected in TgD line mice (*). SW480 was used as a negative control for fusion expression. HT: heart, LV: liver, ...
Supplemental Figure

LG

KD

SP

ST

LV

RT-PCR

HT

Ctrl

TgA21 PCR cycles

TgA21

endo Ros1

x35 (mRos-F/mRos-R) x30 (mEzr-F/mEzr-R)

endo Ezr

x30 (EZR-e10CF1/ROS1-e34-CR1)

EZR-ROS1 fusion Gapdh

x27 (mGapdh-F/mGapdh-R)

SW480

TgC01-LG

TgD07-LG *

TgD06-LG *

TgB22-LG

TgB13-LG

TgA25-LG

TgA21-LG

TgA21-KD

TgA21-ST

Western

TgA21-SP

Lung

endo ROS1

EZR-ROS1 fusion

endo Ezrin

b-actin

Figure S4 Gene expressions in transgenic mice. Expression of the genes indicated at left side was investigated by RT-PCR or immunoblot analysis. In RT-PCR, PCR cycles to amplify target genes were indicated at right side. Ezrin showed ubiquitous endogenous expression, however endogenous Ros1 expression was low. No expression of EZR-ROS1 fusion protein was detected in TgD line mice (*). SW480 was used as a negative control for fusion expression. HT: heart, LV: liver, ST: stomach, SP: spleen, KD: kidney, LG: lung. RT-PCR Primers: mRos-F: TTTCGAGTGGTTTGGATCTTC mRos-R: GATAGAAAACCTGTAGGTGG mEzr-F: ACGAGGAGAAGCGGATCACA mEzr-R: AATCTGACCTGTTTGGCACTA EZR-e10-CF1: GAAAAGGAGAGAAACCGTGGAG ROS1-e34-CR1: TCAGTGGGATTGTAACAACCAG mGapdh-F: AGCTTGTCATCAACGGGAAG mGapdh-R: CCTGCTTCACCACCTTCTTG