final program & abstract book

5 - 8 November 2013 � Dusit Thani Hotel, Manila, Philippines

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5 - 8 November 2013 Dusit Thani Hotel Manila, Philippines

FINAL PROGRAM & ABSTRACT BOOK

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www.rice-genetics.com


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Organizer

5 - 8 November 2013 Dusit Thani Hotel Manila, Philippines

FINAL PROGRAM & ABSTRACT BOOK Organised by:

Managed by:

FINAL PROGRAM & ABSTRACT BOOK iii

www.rice-genetics.com


ACKNOWLEDGEMENTS We would like to thank our sponsor, DuPont Pioneer, for making this Symposium possible. Sponsor:

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TABLE OF CONTENTS


WELCOME LETTER

WELCOME LETTER..........................................................................................vii ABOUT THE ORGANIZERS............................................................................. viii GENERAL INFORMATION..................................................................................ix ONSITE REGISTRATION INFORMATION....................................................... xiii VENUE FLOOR PLAN......................................................................................xiv PROGRAM AT A GLANCE................................................................................xvi POSTER PRESENTATIONS..............................................................................xx SCIENTIFIC PROGRAM

Tuesday, November 05, 2013.................................................................. 1

Wednesday, November 06, 2013............................................................. 6

Thursday, November 07, 2013............................................................... 18

Posters Sessions................................................................................... 29

Abstracts...............................................................................................111 SPONSORS & EXHIBITORS

Sponsors’ Profiles................................................................................ 491

Exhibition Opening Hours.................................................................... 492

Exhibitors List ...................................................................................... 492

Exhibitors’ Profiles................................................................................ 493

AUTHORS INDEX........................................................................................... 499

Dear fellow researchers, scientists and industry partners, On behalf of the International Rice Research Institute, it is my pleasure to welcome you to the 7th International Rice Genetics Symposium (RG7) in Manila, Philippines. In pursuit of IRRI’s aim to disseminate knowledge and skills and to promote discussions on important issues relevant to rice science, we hope to again provide an important forum for leading experts in the fields of classical genetics and genomics to come together and share their expertise to solve the myriad problems that the rice production sector is currently facing. Leading researchers and key opinion leaders in rice breeding and genetics, genomics, genetic resources and evolution, pathology, and grain quality have been invited to facilitate sessions and deliver lectures and I believe this will set a world-class and quality symposium experience for all. The Organizing Committee has planned a series of interactive business and educational activities for your participation over the next few days. There will be abundant learning opportunities at the plenary and concurrent sessions, as well as during the poster presentations. Participants can also look forward to expanding their network at the Symposium dinner and the IRRI field tour. Apart from an exciting scientific program and exhibition showcase, we also welcome participants to embrace and enjoy what Manila has to offer – its unique blend of contemporary and traditional ways of life as well as its cultural landmarks, attractions, and entertainment. We wish to thank all participants including delegates, sponsors and exhibitors for making this Symposium possible. Our continued partnership with the local and international rice industry is what keeps the science of our field moving. Thank you and I wish everyone a fruitful meeting and an enjoyable stay in Manila. Yours faithfully, Eero Nissila Convenor 7th International Rice Genetics Symposium 2013

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ABOUT THE ORGANIZERS

GENERAL INFORMATION

International Rice Research Institute (IRRI) The International Rice Research Institute (IRRI) is a non-profit independent research and training organization. IRRI is a member of the CGIAR Consortium.

Venue Dusit Thani Manila Ayala Centre, 1223 Makati City Metro Manila, Philippines Telephone: +63 (2) 238 8888 Fax: +63 (2) 238 8800

IRRI develops new rice varieties and rice crop management techniques that help rice farmers improve the yield and quality of their rice in an environmentally sustainable way. We work with our public and private sector partners in national agricultural research and extension systems in major rice-growing countries to do research, training, and knowledge transfer.

Language The official language of RG7 is English.

Our social and economic research also informs governments to help them formulate policy to improve the equitable supply of rice.

Time Local time is GMT plus 8 hours.

Our Mission To reduce poverty and hunger, improve the health of rice farmers and consumers, and ensure environmental sustainability through collaborative research, partnerships, and the strengthening of national agricultural research and extension systems.

Climate Manila has a tropical climate with year round temperatures averaging 28°C. High humidity can be expected.

Our Goals • Reduce poverty through improved and diversified rice-based systems. • Ensure that rice production is sustainable and stable, has minimal negative environmental impact, and can cope with climate change. • Improve the nutrition and health of poor rice consumers and rice farmers. • Provide equitable access to information and knowledge on rice and help develop the next generation of rice scientists. • Provide rice scientists and producers with the genetic information and material they need to develop improved technologies and enhance rice production. Local Organizing Committee (LOC) Convenor Member

Dr. Eero A.J. Nissila Dr. Ruaraidh Sackville Hamilton Dr. Nese Sreenivasulu Dr. Abdelbagi Ismail Dr. Hei Leung Dr. Kenneth McNally Dr. Kshirod K. Jena Dr. Glenn Gregorio Dr. Ajay Kohli Dr. Inez Slamet-Loedin Dr. Casiana Vera Cruz Dr. Michael Thomson Dr. Bertrand Collard viii

Voltage 220 volts a/c is the common standard. 110 volts a/c is also used, especially in major hotels. Currency The currency in the Philippines is the Peso (PhP) and the Centavo. US$1 is approximately PHP43.55. Foreign currency may be exchanged at your hotel, and in most of the large department stores, banks and authorized money changing shops. Exchanging money anywhere else is illegal and the laws are strictly enforced. All major credit cards including American Express, Visas and MasterCard are accepted at most large stores, restaurants, hotels and resorts. Badge Entry into the Symposium and related functions are strictly by badge only. Participants are advised to register early to collect their badges and conference kits.

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Registration and Information Desks Registration and information desks are located at outside the Grand Ballroom foyer, Ground Level. Registration opening hours are as follows: Date

Opening Hours (Hrs)

Monday, 4 November

15:00 – 18:30

Tuesday, 5 November

08:00 – 18:30

Wednesday, 6 November

07:00 – 18:30

Thursday, 7 November

07:00 – 18:30

Friday, 8 November

CLOSED

Exhibition Opening Hours The Symposium exhibition is located at the Grand Ballroom foyer, Ground Level. Please refer to the opening hours of the exhibition below. Date

Opening Hours (Hrs)

Tuesday, 5 November

09:00 – 18:30


08:00 – 18:30


08:00 – 18:30

Friday, 8 November

CLOSED

Refreshments Morning and afternoon tea will be served at the exhibition area (Grand Ballroom foyer, Ground Level) as well as outside Molave Room (Mezzanine Floor) and Boardroom 2 (Second Floor) during concurrent sessions. Lunch Buffet lunch is served at two locations: (1) the Poolside Pavilion at Level 2 and (2) UMU restaurant at Level 1. Delegates may proceed to either locations to have their lunch. Symposium Dinner All registered participants are invited to enjoy a networking and cultural evening at the Symposium Dinner on Tuesday, 5 November from 19:30hrs at the Grand Ballroom, Ground Level. Post-Symposium Tours Post-Symposium tour options are available for participants to sign up at a separate fee. Participants may approach the Tour Desk outside the Grand Ballroom foyer, Ground Level, for more information. x


Speakers’ Ready Room Upon registration, all speakers are requested to proceed to the Speakers’ Ready Room (Kamagong Room), which is located on the mezzanine floor, to submit your PowerPoint slide presentations. Please note that all PowerPoint slide presentations should be submitted at least 4 hours prior to your session. Date

Opening Hours (Hrs)

Monday, 4 November

15:00 – 18:30

Tuesday, 5 November

08:00 – 18:30


07:00 – 18:30


07:00 – 18:30

Friday, 8 November

CLOSED

Certificate of Attendance Certificates of Attendance are available for collection at the Self-Registration Counters located at the outside the Grand Ballroom foyer from 14:00hrs on Wednesday, 6 November until 18:30hrs on Thursday, 7 November. Messages/Medical Assistance/Lost & Found Please approach the Information Desk for assistance. Smoking Policy Smoking is not allowed within the Symposium venue. Your compliance is greatly appreciated. Airport Tax Passenger Terminal Fee is levied on all passengers embarking for: 1. International travel: PHP 750 2. Domestic travel: PHP 200 Important Numbers Ninoy Aquino Int’l Airport

877 1109

Bureau of Customs

527 4517

Bureau of Immigration

527 3259

Police / Fire / Medical Emergency

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International Operator

108

Local Operator

109

NOTE: It is advisable to always have the telephone number and the address of your embassy or consulate with you. xi


Dialing Code For international calls from Manila, please dial + (country code) (area code) (telephone number). Liability and Insurance The Symposium Secretariat and Organizers cannot accept liability for personal accidents or loss of or damage to private property of participants and accompanying persons. Participants are advised to take out their own personal travel and health insurance for their trip. Congress Organizers For post-Symposium enquiries and information, please contact the Congress Organizers:


ONSITE REGISTRATION INFORMATION Category

Onsite Registration Fee+

International Delegates

USD 650

Local Delegates and NARES Partners

USD 450

IRRI Scholars

USD 250

Field Tour (subject to seat availability)

USD 80

Accompanying Persons (Fee includes a half-day tour and Symposium Dinner on 5 November.)

USD 230

+Delegates who register onsite may not receive all Symposium materials but will be entitled to lunches, tea breaks, as well as entrance to exhibition and Symposium dinner.

20 Kallang Avenue Level 2 PICO Creative Centre Singapore 339411 Tel: +65 6393 0243 Fax: +65 6292 7577 Email: [email protected]

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venue floor plan


venue floor plan

Lobby Level

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ProgRAM AT A GLANcE

Symposium Timetable – Wednesday, November 6, 2013

Symposium Timetable – Tuesday, November 5, 2013 Time


Time

Ballroom A

Grand Ballroom (Ballroom A & B)

09:15-09:30

Welcome and Introduction

09:30-10:00

Opening Remarks

10:00-10:35

Keynote Presentation: Accelerating crop improvement for sustainable development

10:35-11:45

Plenary Session 01: Linking genome wide association studies (GWAS) and plant breeding to better utilize natural variation in rice Ecology and Cultural History made rice: adaptive episodes, diversification and reticulation over 8000 years

12:00-1315

Lunch

13:15-15:00

Plenary Session 02: Functional and nutritional traits from wild rices What we’ve learned and are still learning about using molecular markers to improve grain yield in maize

Coffee Break

15:30-16:40

Plenary Session 03: Cloning and application of a series of high-yield genes IPA1/DEP1/ GW8 in rice RNA Molecular Machines and Protein Making Factories

19:30-21:30

Symposium Dinner

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Molave

Boardroom 2

Ballroom B

Plenary Session 04: Engineering a C4 rice 08:20-10:05

Characterization of brown planthopper resistance genes in rice Genetic improvement of root system architectures to enhance drought avoidance in rice

10:05-10:25

Coffee Break Plenary Session 05: The genetic control of rice yield through gleaning superior genes

10:25-12:10

Bacterial blight of rice: A window to evolution, disease and everything else Genomics-assisted transfer of QTLs for tolerance to drought, flooding and salinity in to Indian mega rice varieties

Next-generation breeding in rice: challenges and perspectives 15:00-15:30

Grand Ballroom

12:10-13:30

Lunch

13:30-15:00

Poster Session 01

15:00-18:20

CS01: Evolutionary Genetics

CS02: Yield Potential

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CS03: Gene Regulation

CS04: Abiotic Stress Tolerance



Symposium Timetable – Thursday, November 7, 2013

Time

Grand Ballroom Ballroom A

Molave

Ballroom B

Symposium Timetable – Friday, November 8, 2013 Boardroom 2

Plenary Session 06: MAGIC populations for genetic research and breeding applications

08:20-10:05

Time 06:45

Depart from Dusit Thani, Manila

08:30

Expected arrival at IRRI

08:45

Welcome by Dr. Robert S. Zeigler, Director General, IRRI Field Tour Station 1: Drought/Anaerobic Germination (AG)/Green Super Rice (GSR) / MAGIC-Multi-parent Advanced Generation Inter-Cross

Molecular dissection of rice innate immunity to the blast fungus Magnaporthe oryzae

Station 2: International Network for Genetic Evaluation of Rice (INGER)/ Genetic Resources Center (GRC)

New whole genome de-novo assemblies of three divergent strains of rice document novel gene space in aus and indica missed by previous reference assemblies 10:05-10:25

09:00-12:00

Coffee Break

Station 5: Novel Gene Resources/Biotic Stress Resistance

15 Million Years of Oryza Evolution as Seen Through the Analysis of 12 High-Quality Genome Assemblies

Station 6: Submergence and Stagnant Water 12:00-13:30

Rice diversity and genetics for future plant breeding

13:30-15:00

12:10-13:30

Lunch

13:30-15:00

Poster Session 02

15:00-18:20

CS05: Biotic Stress Resistance

CS06: Genomic Diversity

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Station 3: Plant Breeding, Genetics and Biotechnology Demo Plot Station 4: Salinity, Pstol/Pup1 and Zn Def Tol

Plenary Session 07: Impacts of rising temperatures on R-gene-mediated resistance in rice 10:25-12:10

Field Tour Program

Lunch Free Schedule: Visit to Genotyping Services Lab, Genetic Transformation Lab and Gene Bank

CS07: CS08: Grain Quality Breeding and Nutrition Applications

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POSTER PRESENTATION A different set of posters will be on display daily from Wednesday, November 6 to Thursday, November 7. Posters are displayed as per the Poster Numbers indicated in the Program. Poster presenters are requested to stand by their posters during the following dates and times:


Please refer to the poster set-up and dismantling schedule below. Kindly remove your posters within the specific dismantling period. The organizers will not be responsible for posters that are not removed on time. Presentation Date

Topics and Dates of Presentation

Poster No.

Display Location

Wednesday, November 6, 2013 13:30 – 15:00 Poster Session 01

Wednesday, November 6

Evolutionary Genetics

P0001-P0019 Mezzanine Level – Poster Area

Yield Potential

P0020-P0051 Lobby Level – Poster Area

Gene Regulation


Abiotic Stress Tolerance


Thursday, November 7, 2013 13:30 – 15:00 Poster Session 02 Biotic Stress Resistance


Genomic Diversity

P0056-P0084 Mezzanine Level – Poster Area

Grain Quality and Nutrition


Breeding Applications


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Set-up Period Wednesday, November 6 From 07:00-08:00hrs Wednesday, November 6 From 12:00-13:30hrs

Dismantling Period

Wednesday, November 6 From 18:30-19:30hrs

Wednesday, November 6 From 19:30-21:30hrs Thursday, November 7

Thursday, November 7 From 07:00-08:00hrs Thursday, November 7 From 12:00-13:30hrs

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Thursday, November 7 From 18:30-19:30hrs

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SCIENTIFIC PROGRAM Tuesday, 5 November 2013


Tuesday, november 05, 2013

PLenary SeSSiOn 01 anD KeynOTe PreSenTaTiOn 09:15 - 12:00

Grand Ballroom

Chairman: e. nissila (Philippines) Time 09:15

Welcome and introduction

09:30

Opening remarks

10:00

Keynote Presentation: accelerating crop improvement for sustainable development A. Dobermann (Philippines)

10:35

PS01-01 Linking genome wide association studies (gWaS) and plant breeding to better utilize natural variation in rice S. McCouch (USA)

11:10

PS01-02 ecology and Cultural History made rice: adaptive episodes, diversification and reticulation over 8000 years D. Fuller (United Kingdom)

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PLenary SeSSiOn 02

PLenary SeSSiOn 03

13:15 - 15:00

Grand Ballroom

15:30 - 16:40

Chairman: J. Hernandez (Philippines)

Chairman: n. Singh (india)

Time 13:15

Time 15:30

13:50

14:25

PS02-01 Functional and nutritional traits from wild rices R. Henry (Australia) PS02-02 What we've learned and are still learning about using molecular markers to improve grain yield in maize R. Bernardo (USA) PS02-03 next-generation breeding in rice: challenges and perspectives M. Yano, E. Yamamoto, J. Yonemaru, R. Mizobuchi, H. Kato, K. Matsubara, J. Tanaka, S. Kobayashi, H. Tsunematsu, T. Yamamoto (Japan)

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16:05

Grand Ballroom

PS03-01 Cloning and application of a series of high-yield genes iPa1/DeP1/gW8 in rice Q. Qian, D. Zeng, G. Zhang, Z. Gao, L. Zhu, J. Hu, J. Xu, G. Dong, X. Hu, D. Xue, L. Guo (China) PS03-02 rna Molecular Machines and Protein Making Factories J. Sáez-Vásquez, F. Pontvianne, N. Durut, M. Abou-Ellail, C. Charbonnel, P. Comella, A. DeBures, E. Jobet, R. Cooke (France)

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SCIENTIFIC PROGRAM Wednesday, 6 November 2013

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Wednesday, november 06, 2013

PLenary SeSSiOn 04 08:20 - 10:05

Grand Ballroom

Chairman: M. yano (Japan) Time 08:20

PS04-01 engineering a C4 rice W.P. Quick (Philippines)

08:55

PS04-02 Characterization of brown planthopper resistance genes in rice G. He, B. Du, R. Chen, S. Jing, L. Zhu (China)

09:30

PS04-03 genetic improvement of root system architectures to enhance drought avoidance in rice Y. Uga (Japan)

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PLenary SeSSiOn 05

COnCUrrenT SeSSiOn 01 - eVOLUTiOnary geneTiCS

10:25 - 12:10

Grand Ballroom

Chairman: r. Bernardo (USa) Time 10:25

11:00

11:35

15:00 - 17:55

Boardroom 2

Convener: D. Fuller (United Kingdom) Co-Convener: r.S. Hamilton (Philippines)

PS05-01 The genetic control of rice yield through gleaning superior genes N. Hirotsu (Japan) PS05-02 Bacterial blight of rice: a window to evolution, disease and everything else F. White (USA) PS05-03 genomics-assisted transfer of QTLs for tolerance to drought, flooding and salinity in to indian mega rice varieties N.K. Singh, R. Singh, Y. Singh, N. Yadav, A.K. Singh, P.C. Sharma, S.L. Krishnamurthy, S.K. Sharma, J.L. Dwivedi, A.K. Singh, V.N. Singh, J.S. Verma, S.P. Singh, P.K. Singh, J. Singh, R. Kumar, Nilanjay, N.K. Singh, T. Ahmad, S.K. Chetia, M. Rai, R. Perraju, D.N. Singh, A. Pandey, T. Mohapatra, N.P. Mandal, J.N. Reddy, O.N. Singh, J.L. Katara, B. Marandi, P. Sawain, R.K. Sarkar, D.P. Singh, S. Verulkar, T. Ram, G. Padmawathi, Y. Suraynarayana, P.R. Rao, M.G. Rani, T. Anuradha, R.M. Kathiresan, A. Anandan, S. Thirumeni, K. Paramsivam, S. Nadarajan, A. Kumar, E. Septiningshih, U.S. Singh, U.S. Ismail (India)

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Time 15:00

CS01-01 Closed panicles in rice domestication T. Ishii, K. Numaguchi, K. Miura, K. Yoshida, T.T. Pham, T.M. Htun, M. Yamasaki, T. Matsumoto, R. Terauchi, R. Ishikawa, M. Ashikari (Japan)

15:25

CS01-02 in Search of vestige of artificial selection in rice J. Lyu, S. Zhang, W. He, J. Zhang, F. Hu, W. Wang (China)

15:50

CS01-03 Bayesian multi-phenotype genome-wide association methods for experimental designs of arbitrary complexity A.J. Greenberg, J. Mezey, J. Jannink, S. McCouch (USA)

16:15

CS01-04 genetic structure of cultivated rice (Oryza sativa L.) and its progenitor, O. rufipogon in india D. Raj R, M. Jose, G. Varghese, P. BC, S. ON, R. JC, G. Thomas (India)

16:40

CS01-05 Origin and ecotypic differentiation of Bangladeshi indica rice N. Hoque, S. Begum, M. Islam, G. Gregorio (Bangladesh)

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COnCUrrenT SeSSiOn 01 - eVOLUTiOnary geneTiCS 15:00 - 17:55

COnCUrrenT SeSSiOn 02 - yieLD POTenTiaL Boardroom 2

15:00 - 17:55

Convener: D. Fuller (United Kingdom) Co-Convener: r.S. Hamilton (Philippines)

Convener: B.C. Viraktamath (india) Co-Convener: K. Jena (Philippines)

Time 17:05

Time 15:00

17:30

CS01-06 Phylogenetic relationship among weedy, wild and cultivated species of Sri Lankan rice based on morphological traits D. Ratnasekera, I.DP. Gangodawatta (Sri Lanka) CS01-07 Patterns of rice diversity from SnP delineated the origin of the atypical O. sativa group in Madagascar from intermediary forms of the indian sub-continent N. Ahmadi, C. Billot, G. Droc, D. Brunel, J. Frouin, A. Ramanantsoanirina, K. McNally, B. Courtois, J. Glaszmann (France)

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Molave

CS02-01 Towards improvement of yield potential in Basmati rice: from mapping to marker assisted selection A. Singh, S. Gopala Krishnan, P. Bhowmick, K. Prabhu, T. Mohapatra, N. Singh, T. Sharma, M. Nagarajan, K. Vinod (India)

15:25

CS02-02 identification of a gene involving in rice grain yield loss by air pollution gas, ozone M. Tamaoki (Japan)

15:50

CS02-03 Quantitative trait loci and their interactions regulating the flowering time variation and photoperiodic response in rice P. Subudhi, T. DeLeon, R. Karan, P. Singh, A. Parco (USA)

16:15

CS02-04 identification and characterization of QTL involving rate of photosynthesis in high yielding rice T. Takai (Japan)

16:40

CS02-05 Molecular mapping and introgression of novel yield enhancing QTLs from O. longistaminata B. Marathi, J.M. Ramos, K.K. Jena (Philippines)

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COnCUrrenT SeSSiOn 02 - yieLD POTenTiaL 15:00 - 17:55

COnCUrrenT SeSSiOn 03 - gene regULaTiOn Molave

15:00 - 17:55

Convener: B.C. Viraktamath (india) Co-Convener: K. Jena (Philippines)

Convener: T. Mohapatra (india) Co-Convener: a. Kohli (Philippines)

Time 17:05

Time 15:00

17:30

CS02-06 genetic improvement of the yield potential of indica-type rice by using japonica-type varieties T. Ishimaru, D. Fujita, K. Sasaki, M. Obara, S. Yanagihara, Y. Fukuta, N. Kobayashi (Philippines) CS02-07 introgression of erect panicle genes (QTL) from aa genome species to increase yield potential in rice (Oryza sativa) D. Tao, J. Zhou, Z. Wu, P. Xu, J. Li, Y. Zhang, X. Deng, F. Hu, W. Deng, Y. Yang (China)

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Ballroom B

CS03-01 Jasmonate-dependent transcriptional regulation in response to different environmental cues M. Riemann, K. Svyatyna, R. Dhakarey, M. Hazman, R. Brendel, P. Nick (Germany)

15:25

CS03-02 genome-Wide Discovery of cis-elements in Promoter Sequences N. Alexandrov (Philippines)

15:50

CS03-03 Tiny rnas big impact on genome regulation A. Jha (India)

16:15

CS03-04 novel gene expression Tools for rice Biotechnology R. Thilmony, M. Cook, M.E. Guttman (USA)

16:40

CS03-05 role of Microrna-guided Post-transcriptional gene regulation in Plants R. Sunkar (USA)

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COnCUrrenT SeSSiOn 03 - gene regULaTiOn 15:00 - 17:55

COnCUrrenT SeSSiOn 04 - aBiOTiC STreSS TOLeranCe Ballroom B

15:00 - 17:55

Convener: T. Mohapatra (india) Co-Convener: a. Kohli (Philippines)

Convener: n. Singh (india) Co-Convener: a. ismail (Philippines)

Time 17:05

Time 15:00

17:30

CS03-06 Update on gramene database and a study of the rice transcriptome under salt stress S. Naithani, M.K. K Monaco, S. Wei, J. Stein, P. Dharmawardhana, J. Preece, V. Amarasinghe, Y. Jiao, S. Kumari, S. Pasternak, A. Olson, J. Thomason, K. Youens-Clark, G. Wu, P. D’Eustachio, D. Bolser, A. Kerhornou, S. Fox, C. Sullivan, M. Keays, R. Petryszak, H. Parkinson, L. Stein, P. Kersey, D. Ware, P. Jaiswal (USA) CS03-07 improving salinity tolerance by understanding the significance of allelic variants in HKT1;5 S. Negrao, S.T. Arold, I.S. Pires, I.A. Abreu, M.M. Oliveira, M. Tester (Saudi Arabia)

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Ballroom A

CS04-01 restructuring of root architecture is a Key to engineer Drought Tolerance J. Kim, J.S. Jeong, Y.S. Kim (Korea)

15:25

CS04-02 a novel pathway mediated by SnaC1-OsSrO1c-DST promotes stomata closure for drought resistance in rice L. Xiong, J. You, Hu H., K. Songyikhangsuthor, Z. Guo (China)

15:50

CS04-03 raising salinity and drought tolerant rice using forward and reverse genetics approaches A. Pareek, N. Lakra, P. Soni, A. Sharan, K. Anwar, J.K. Tripathy, S.L. Singla-Pareek (India)

16:15

CS04-04 Salt stress amelioration in rice through manipulation and regulation of inositol metabolism A. Lahiri Majumder (India)

16:40

CS04-05 advances in the development of iron-toxicity tolerant rice varieties for resource-poor farmers in sub Saharan africa K.N. Dramé, R. Venuprasad, A. Konaté, M.L. Barry, A. Maji, R. Rabeson, J.M. Anguete, K. Dartey, K. Saito, S. Mouritala, M. Sie (Tanzania)

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COnCUrrenT SeSSiOn 04 - aBiOTiC STreSS TOLeranCe 15:00 - 17:55

Ballroom A

Convener: n. Singh (india) Co-Convener: a. ismail (Philippines) Time 17:05

17:30

CS04-06 Physiological and genetic advances to unravel heat stress responses in rice J. Krishna, C. Ye, I. Tsutomo, B.N. Rajeev, R. Edilberto (Philippines) CS04-07 How does the molecular machinery of rice seedlings 'react' to heat stress? A. Grover (India)

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SCIENTIFIC PROGRAM Thursday, 7 November 2013

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Thursday, november 07, 2013

PLenary SeSSiOn 06 08:20 - 10:05

Grand Ballroom

Chairman: F. White (USa) Time 08:20

PS06-01 MagiC populations for genetic research and breeding applications H. Leung, N. Bandillo, C. Raghavan, E. Huang, R. Mauleon, M. Thomson, E. Redona, R.K. Singh, G. Gregorio (Philippines)

08:55

PS06-02 Molecular dissection of rice innate immunity to the blast fungus Magnaporthe oryzae G. Wang (USA)

09:30

PS06-03 new whole genome de-novo assemblies of three divergent strains of rice document novel gene space in aus and indica missed by previous reference assemblies R.W. McCombie (USA)

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PLenary SeSSiOn 07

COnCUrrenT SeSSiOn 05 - BiOTiC STreSS reSiSTanCe

10:25 - 12:10

Grand Ballroom

Chairman: S. McCouch (USa) Time 10:25

15:00 - 18:20

Ballroom B

Convener: J. Leach (USa) Co-Convener: C. Vera Cruz (Philippines) Co-Convener: K. Jena (Philippines)

PS07-01 impacts of rising temperatures on r-gene-mediated resistance in rice J.E. Leach, R. Corral, H. Liu, S.K. Srivastava, J.F. Balidon, I.R. Choi, S. Kikuchi, R. Mauleon, C. Jahn, A. Pereira, C. Vera Cruz, V. Verdier (USA)

11:00

PS07-02 15 Million years of Oryza evolution as Seen Through the analysis of 12 High-Quality genome assemblies R. Wing (USA)

11:35

PS07-03 rice diversity and genetics for future plant breeding R.S. Zeigler (Philippines)

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Time 15:00

CS05-01 Cloning and characterization of BPH18 gene conferring BPH resistance in rice H. Ji, Y. Kim, Suh J., H. Park, S. Kim, S. Kim, H. Kim, G. Lee, U. Yoon, T. Kim, G. An, K.K. Jena (Korea)

15:25

CS05-02 Simultaneous Transfer and Mapping of novel Bacterial Blight resistance genes from Oryza glaberrima and O. rufipogon to O. sativa K. Singh, B. Kaur, J.S. Lore, G.K. Sahi, D. Bhatia, R. Mahajan, V. Gupta, N. Singh, G. Singh, D.S. Brar (India)

15:50

CS05-03 Co-differentiation of blast races and resistance of rice in global level Y. Fukuta (Japan)

16:15

CS05-04 Bacterial Blight of rice: From Population Structures to Tailored resistance genes in africa S. Cunnac, A. Pérez-Quintero, T.T. Tuan, I. Wonni, M. Hutin, L. Poulin, H. Doucouré, C. Pesce, F. Auguy, S. Baufumé, V. Verdier, B. Szurek, R. Koebnik (France)

16:40

CS05-05 Function, evolution, interaction and utilization of rice blast Pik alleles Q. Pan, C. Zhai, Y. Zhang, L. Hua, B. Yuan, N. Yao, F. Lin, Z. Liu, R. Chai, W. Liu, L. Wang (China)

20



COnCUrrenT SeSSiOn 05 - BiOTiC STreSS reSiSTanCe 15:00 - 18:20

COnCUrrenT SeSSiOn 06 - genOMiC DiVerSiTy Ballroom B

Convener: J. Leach (USa) Co-Convener: C. Vera Cruz (Philippines) Co-Convener: K. Jena (Philippines) Time 17:05

17:30

17:55

CS05-06 Molecular Breeding for Durable bacterial blight resistance in rice R.M. Sundaram, G.S. Laha, B.C.V. Viraktamath, M.S. Madhav, S.M. Balachandran, N. Shobha Rani, P. Natarajkumar, K. Sujatha, S.K. Hajira, A. Yugander, M.R.V. Priya, R.V. Sonti (India) CS05-07 recent advances in Leafhopper and Planthopper resistance in rice H. Yasui, D. Fujita, J. Jairin, A. Yoshimura, M. Matsumura, (Japan) CS05-08 Detection of novel QTL and major gene associated with leaf blast resistance and introgression of resistance in drought tolerant rice cultivars S. Hittalmani, G. Uday (India)

21

15:00 - 17:55

Boardroom 2

Convener: r. Wing (USa) Co-Convener: K. Mcnally (Philippines) Time 15:00

CS06-01 assessment of root trait variation under phosphorus deficiency through genome-Wide association analysis M. Wissuwa, T. Fukuda, J. Pariasca-Tanaka, A. Mori (Japan)

15:25

CS06-02 genetic change of Pyricularia grisea in different host genome U.W. Suharsono, S. Listiyowati, G. Rahayu, A. Hartana (Indonesia)

15:50

CS06-03 The griSP global rice Phenotyping network: a Multienvironment Program to Tap into Useful genetic Diversity M. Dingkuhn, J.M. Pasuquin, M.B. Ines, R. Laza, K. McNally (Philippines)

16:15

CS06-04 Utilization of active MiTe mPing as a novel genetic tool for modification of stress response in rice K. Yasuda, T. Tsukiyama, H. Saito, K. Naito, M. Teraishi, T. Tanisaka, Y. Okumoto (Japan)

16:40

CS06-05 genetic Diversity of Upland rice Landraces and Traditional Varieties of Bukidnon, Philippines J.M. Jamago, M.J.N. Fuentes, R.V. Cortes (Philippines)

22



COnCUrrenT SeSSiOn 06 - genOMiC DiVerSiTy 15:00 - 17:55

COnCUrrenT SeSSiOn 07 - grain QUaLiTy anD nUTriTiOn Boardroom 2

Convener: r. Wing (USa) Co-Convener: K. Mcnally (Philippines) Time 17:05

17:30

15:00 - 18:20

Molave

Convener: r. Henry (australia) Co-Convener: i. Slamet-Loedin (Philippines) Co-Convener: n. Sreenivasulu (Philippines)

CS06-06 Harnessing functional genomics for crop improvement through TiLLing C.P. Moehs, D. Facciotti, C. McGuire, D. Loeffler, V. Knauf (USA) CS06-07 international rice informatics Consortium N. Alexandrov, Mauleon. R, K. McNally, N.R. Sackville Hamilton, H. Leung (Philippines)

23

Time 15:00

CS07-01 Healthier rice Varieties: an approach to reducing dietary inadequacy of micronutrients. G. Barry (Philippines)

15:25

CS07-02 iron and zinc biofortification in mega variety I.H. Slamet-Loedin, K.R. Trijatmiko, C. Duenas Jr, P. Francisco, F.M. Arines, C. Adeva, J. Balindong, N. Oliva, M.V. Sapasap, L. Torrizo, J. Borero, E. Lombi, E. Tako, R.P. Glahn, J. Stangoulis, P. Chadha-Mohanty, A.A.T. Johnson, J. Tohme, G. Barry (Philippines)

15:50

CS07-03 Marker-assisted discovery and utilization of gene/QTLs associated with grain Quality Traits in aromatic rices in india N. Shobha Rani, M. Sheshu Madhav, R.M. Sundaram, G.S. Varaprasad, K. Suneetha, G.S. Laha, M. Srinivas Prasad, A.K.P. Sivaranjani, S. Srikanth, B.C. Viraktamath (India)

16:15

CS07-04 Cadmium transporters in rice H. Nakanishi, Y. Ishimaru, K. Bashir, R. Takahashi, S. Ishikawa, N.K. Nishizawa (Japan)

16:40

CS07-05 Production of rice bran oil with improved fatty acid composition and stability S. Rahman, E.S. Zaplin, G.J. Tiwari, A. Aumeeruddy, V.M. Butardo, C. Blanchard, Q. Liu, Z. Li (Malaysia)

24



COnCUrrenT SeSSiOn 07 - grain QUaLiTy anD nUTriTiOn 15:00 - 18:20

Molave

Convener: r. Henry (australia) Co-Convener: i. Slamet-Loedin (Philippines) Co-Convener: n. Sreenivasulu (Philippines) Time 17:05

17:30

17:55

COnCUrrenT SeSSiOn 08 - BreeDing aPPLiCaTiOnS

CS07-06 rice Bran: a food ingredient with global Public Health Opportunity for disease prevention and crop improvement strategies E.P. Ryan, A. Kumar, E.C. Borresen, A. Goodyear, J. Leach, S. Dow (USA) CS07-07 Molecular Dissection of Starch grain Size Control in rice endosperm R. Matsushima, M. Maekawa, N. Fujita, Y. Kawagoe, W. Sakamoto (Japan) CS07-08 a multi-platform metabolomics and genomics approach to identify new quality traits in rice M. Calingacion, C. Boualaphanh, V.D. Daygon, R. Mumm, A. Erban, J. Kopka, T. Hansen, J. Schoerring, J. Ward, M. Fitzgerald, R. Hall (Philippines)

25

15:00 - 17:55

Ballroom A

Convener: n. Manigbas (Philippines) Co-Convener: B. Collard (Philippines) Time 15:00

CS08-01 Overview of molecular breeding activities at the africa rice Centre V. Ramaiah, B. Kofi, B. Manneh, K.N. Drame, N. Zenna, M. Sow, M. Ndjiondjop (Nigeria)

15:25

CS08-02 California rice breeding: the first 100 years K.S. McKenzie, V.C. Andaya, F. Jodari, S.O. Samonte, J.J. Oster, C.B. Andaya (USA)

15:50

CS08-03 Prediction of cooked rice taste using diagnostic Dna markers H. Koh, P. Lestari (Korea)

16:15

CS08-04 Using the ‘Rice Diversity Panel 1’ to develop novel germplasm for breeding G.C. Eizenga, A.M. McClung, M.H. Wright, A.J. Greenberg, J.N. Cobb, J.K. Jung, R.T. Clark, N. Singh, F.J. Agosto-Perez, G.A. DeClerck, E. Shakiba, S. Ahn, L.V. Kochian, J.G. Mezey, S.R. McCouch (USA)

16:40

CS08-05 accuracy of genomic selection in a rice synthetic population developed for recurrent selection C. Grenier, T.V. Cao, Y. Ospina, J. Tohme, B. Courtois, N. Ahmadi (Colombia)

26



COnCUrrenT SeSSiOn 08 - BreeDing aPPLiCaTiOnS 15:00 - 17:55

Ballroom A

Convener: n. Manigbas (Philippines) Co-Convener: B. Collard (Philippines) Time 17:05

17:30

CS08-06 High-Throughput SnP genotyping for Breeding applications in rice M.J. Thomson, C.J. Dilla-Ermita, M.Y. Reveche, G. Malitic, M. Dwiyanti, V. Juanillas, R.P. Mauleon, J.H. Chin, C. Vera Cruz, B. Collard, M. Wright, S. McCouch, E. Nissila (Philippines) CS08-07 rice molecular breeding applications at Philrice A.A. Alfonso, D.A. Tabanao, L.M. Perez, O.E. Manangkil, N.L. Manigbas, R.R. Suralta, N.V. Desamero, E.C. Arocena, T.F. Padolina (Philippines)

27

POSTER SESSION

1


Wednesday, november 06, 2013 POSTer SeSSiOn

13:30 - 15:00

Poster Area

eVOLUTiOnary geneTiCS Poster No F1 POLLen STeriLiTy FOUnD in HyBriD PrOgeny BeTWeen P0001 CULTiVaTeD anD WiLD SPeCieS OF THe aa genOMe SPeCieS in riCe Y. Yamagata, K.T. Win, N.N. Giao, M. Sakata, N. Takano-Kai, A. Yoshimura (Japan) P0002

TWO LinKeD geneS On riCe CHrOMOSOMe 2 inDePenDenTLy inDUCe F1 POLLen STeriLiTy in a HyBriD BeTWeen Oryza SaTiVa anD O. gLUMaePaTULa M. Sakata, Y. Yamagata, S. Sobrizal, K. Doi, A. Yoshimura (Japan)

P0003

QTL anaLySiS OF POLLen STeriLiTy USing TWO BC1F1 POPULaTiOnS DeriVeD FrOM BaCKCrOSSing WiTH BOTH ParenTS, aSian anD aFriCan CULTiVaTeD riCe SPeCieS K. WIN, C. Ogata, Y. Yamagata, H. Yasui, A. Yoshimura (Japan)

P0004

g?g anD g?e inTeraCTiOn OF THree geneS FOr HyBriD STeriLiTy BeTWeen Oryza SaTiVa anD Oryza gLaBerriMa J. Li, M. He, J. Zhou, P. Xu, Y. Zhang, X. Deng, W. Deng, Y. Yang, Z. Wu, D. Tao (China)

P0005

Fine MaPPing OF inTerSPeCiFiC HyBriD STeriLe gene S44(T) BeTWeen Oryza SaTiVa anD O. LOngiSTaMinaTa Y. Zhang, Y. Yang, J. Li, J. Zhou, P. Xu, X. Deng, W. Deng, F. Hu, Z. Wu, D. Tao (China)

P0006

riCe in PreHiSTOriC THaiLanD C. Cobo Castillo (United Kingdom)

29





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

eVOLUTiOnary geneTiCS (contd.) Poster No a CLOSeD PaniCLe TraiT regULaTeD By OSLg1 P0007 in DOMeSTiCaTeD riCe, Oryza SaTiVa L. R. Ishikawa, K. Miura, K. Numaguchi, K. Yoshida, T.M. Htun, P.T. Thanh, T. Matsumoto, M. Yamasaki, R. Terauchi, M. Ashikari, T. Ishii (Japan) P0008

DeTeCTiOn OF DiFFerenT eXOn-inTrOn STrUCTUreS BeTWeen CULTiVaTeD riCe T. Tanaka, G. Haberer, K. Mayer (Japan)

P0009

geOgraPHiC VariaTiOn PaTTernS in aSia PaCiFiC Oryza SerieS SaTiVae M.C.N. Banaticla-Hilario, N.R. Sackville Hamilton, R.G. Van den Berg, K.L. McNally (Philippines)

P0010

a DraFT genOMe aSSeMBLy anD COMParaTiVe TranSCriPTOMiCS OF THe aFriCan WiLD riCe SPeCieS Oryza LOngiSTaMinaTa X.I.N. Li, X.I.N. Liu, J.I.N.G. Cai, L. Li, X.U.N. Xu, Y.A.N.G. Dong, Y. Zhang, W.E.N. Wang, F. Hu (China)

P0011

CHaKHaO riCe LanDraCeS (Oryza SaTiVa L.) OF nOrTH-eaST inDia: COLLeCTiOn anD CHaraCTerizaTiOn OF THe geneTiC DiVerSiTy S. Roy, A. Banerjee, A. Pattanayak, A.K. Misra, S.V. Ngachan, K.C. Bansal (India)

30

13:30 - 15:00

Poster Area

eVOLUTiOnary geneTiCS (contd.) Poster No naTUraL VariaTiOn OF aVr-PiTa1 in MagnaPOrTHe Oryzae P0012 in yUnnan OF CHina J. Li, C. Li, Y. Jia (China) P0013

a nOn-LOBeD MeSOPHyLL CeLL MUTanT OF riCe: a COMParaTiVe anaLySiS OF LeaF anaTOMiCaL STrUCTUre anD PHOTOSynTHeTiC ParaMeTerS TO iDenTiFy THe rOLe OF LeaF LOBBing in riCe A. Mabilangan, W.P. Quick, A.M. Delos Reyes, S.M. Yu, S.F. Lo, T.L. Sage (Philippines)

P0014

anaLySiS OF MOrPHOLOgiCaL VariaTiOn aMOng ACCESSIONS OF TRADITIONAL RICE “KALU HEENATI” S. Geekiyanage, K.G.P.B. Karunarathne, E.U.U. Rathnathunga, S. Senaweera, N. Dissanayake, K. Fernando (Sri Lanka)

P0015

DiVerSiTy OF TraDiTiOnaL riCe VarieTieS COnSerVeD in PHiLriCe geneBanK BaSeD On agrO-MOrPHOLOgiCaL TraiTS M.I.C. Calayugan, C.L. Diaz, M.C. Ferrer, L.M. Perez (Philippines)

P0016

genOMiC FOSSiLS OF riCe ParareTrOVirUSeS reTraCe HOST DOMeSTiCaTiOn HiSTOry S. Chen, R. Liu, K.O. Koyanagi, J.R. Encabo, I.R. Choi, Y. Kishima (Japan)

31





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

Poster No

Poster No

P0018

P0019

Poster Area

yieLD POTenTiaL (contd.)

eVOLUTiOnary geneTiCS (contd.)

P0017

13:30 - 15:00

P0020

COMPaCT PaniCLe arCHiTeCTUre LiMiTS grOWTH anD SUCrOSe SynTHaSe aCTiViTy OF DeVeLOPing riCe KerneLS. P. Mohapatra, B. Panda, R. Panigrahi, E. Kariali, B. Shaw (India)

P0021

gene DiSCOVery FOr engineering C4 riCe: a reVerSe geneTiCS aPPrOaCH FOr UnDerSTanDing Kranz anaTOMy K. Acebron, N. Larazo, R. Garcia, A. Mabilangan, P. Pablico, A.N. Monroy, V. Thakur, S. Wanchana, G. Rizal, M.J. Dionora, W.P. Quick (Philippines)

eVaLUaTiOn On yieLD, PHySiCaL anD CHeMiCaL PrOPerTieS OF MarDi'S aDVanCeD riCe LineS E. Sunian, A. Ramli, O. Omar, M. Hashim, A. Mokhtar, Z. Hussain, N. Abd Karim, M. Ab Ghaffar, M. Jamal, S. Shamsul Amri (Malaysia)

P0022

CenTrOMere eVOLUTiOn aCCOMPanieD By inVerSiOnS anD TranSPOSiTiOnS in THe Oryza SPeCieS Y. Liao, B. Li, T. Liu, J. Chen, X. Zhang, M. Chen (China)

QTL MaPPing FOr yieLD TraiTS anD THeir MiD-ParenTaL HeTerOSiS in inDiCa riCe Y. Zhu, D. Mei, J. Ying, D. Huang, Y. Fan, J. Zhuang (China)

P0023

HarneSSing TranSgreSSiVe SegregaTiOn OF a MULTiParenT aDVanCeD generaTiOn inTer-CrOSS (MagiC) POPULaTiOn FOr yieLD iMPrOVeMenT N. Bandillo, I. Lobina, A. Sajise, G. Gregorio, E. Redoña, C. Raghavan, R.K. Singh, H. Leung (Philippines)

P0024

iDenTiFiCaTiOn OF genOTyPeS, genOMiC regiOnS anD geneS FOr niTrOgen USe eFFiCienCy in riCe C.N. Neeraja, S.R. Voleti, K. Surekha, D. Subrahmanyam, I. Subhakararao, B. Srikanth, P. Vijayalakshmi, N. Sailaja, P. Revathi, B.C. Viraktamath (India)

aLTeraTiOnS in PHOTOSynTHeTiC TraiTS in C3 anD C4 PLanTS M.J.A. DIONORA, J. Chatterjee, R. Mogul, K. Acebron, G. Dimayuga, J. Ranada, I. Canicosa, W.P. Quick (Philippines)

32

33





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00



Poster No

Poster No

Poster Area

P0025

CHaraCTerizaTiOn OF inTrOgreSSiOn LineS WiTH an inDiCaTyPe riCe (Oryza SaTiVa L.) VarieTy ir64 geneTiC BaCKgrOUnD M. Uddin, M. Obara, S. Yanagihara, N. Kobayashi, T. Ishimaru, Y. Fukuta (Japan)

P0030

iDenTiFiCaTiOn anD CHaraCTerizaTiOn OF a MaJOr QTL FOr rOOT eLOngaTiOn UnDer VariOUS COnCenTraTiOn OF niTrOgen M. Obara, T. Ishimaru, D. Fujita, N. Kobayashi, S. Yanagihara, Y. Fukuta (Japan)

P0026

riCe CrOP eCOPHySiOLOgy in UrUgUay: PrODUCTiViTy anD iTS inTeraCTiOn WiTH CLiMaTiC FaCTOrS F. Pérez de Vida (Uruguay)

P0031

MeTHOD OF FiXing geneS COMPLeX DeFining HeTerOTiC eFFeCT J. Goncharova, E. Kharitonov (Russia)

P0027

MaPPing OF QTLS aSSOCiaTeD WiTH grain TyPe TraiTS USing THree POPULaTiOnS FrOM XieyOU9308, a SUPer HyBriD OF riCe D. Chen, Y. Zhang, L. Cao (China)

P0032

MOLeCULar BreeDing OF green SUPer riCe (gSr) VarieTieS WiTH enHanCeD grain yieLD POTenTiaLS anD MULTiPLe BiOTiC STreSS TOLeranCeS J. Ali, J.L. Xu, Y.M. Gao, C.M. Marfori, J.E. Revilleza, Z.K. Li (Philippines)

P0028

iDenTiFiCaTiOn OF QrL7, a MaJOr QUanTiTaTiVe TraiT LOCUS aSSOCiaTeD WiTH riCe rOOT LengTH in HyDrOPOniC COnDiTiOnS Y. Zhang, H. Wang, X. Xu, L. Cao (China)

P0033

P0029

COMBining aBiLiTy TeSTS On S1 LineS FrOM reSTOrer POPULaTiOn. a TOOL TO enHanCe eFFiCienCy OF HyBriD BreeDing anD FOrMing Or reinFOrCing HeTerOTiC grOUPS. J. TAILLEBOIS, J.P. DOSSMANN (Brazil)

aLLeLiC VariaTiOn OF rePOrTeD CanDiDaTe geneS FOr yieLD in Oryza gLaBerriMa, STeUDeL in COMPariSOn WiTH Oryza SaTiVa, L J. Newmah, A. Marshall, N.A. Ansari, T. Ram, I. Subhakara Rao, C.N. Neeraja (India)

P0034

STUDy On THe geneTiC VariaTiOn, COrreLaTiOn anD PaTH anaLySiS BeTWeen inTer-SUBSPeCieS riCe riLS anD iTS BaCKCrOSS grOUP Y. Xin, Z. Zhang (China)

34

35





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00



Poster No

Poster No

P0035

BreeDing riCe FOr inCreaSeD nUMBer OF SiPKeLeTS Per PaniCLe M. Norouzi, A. Nabipour (Iran)

P0036

geneTiC engineering TO inTrODUCe C4 PHOTOSynTHeTiC geneS inTO riCe S. Karki, S. Covshoff, H. Lin, F. Montecillo, J. Reyes, M. Acoba, R. Tapia, F. Danila, J. Sagun, G. Lorenzana, M. Rosa, A. De Luna, I. SlametLoedin, J. Hibberd, W. Quick (Philippines)

P0037

QTL anaLySiS FOr HigHer OrDer BranCHing OF PaniCLe in riCe inCreaSing grain nUMBer Y. Fujishiro, T. Hobo, M. Ikeda, H. Kitano (Japan)

P0038

MaPPing anD VaLiDaTiOn OF QUanTiTaTiVe LOCi FOr SPiKeLeTS Per PaniCLe anD grain WeigHT in riCe D.M. Kim, J.W. Kang, H.S. Lee, S.N. Ahn (Korea)

P0039

eVaLUaTiOn OF yieLD anD yieLD COMPOnenTS OF ir64 inTrOgreSSiOn LineS WiTH LaTe DayS TO HeaDing QTLS P. Lumanglas, K. Sasaki, T. Ishimaru, T. Ishimaru (Philippines)

P0040

aLLeLiC DiVerSiTy OF yieLD-enHanCing geneS in inDiCa anD JaPOniCa riCe CULTiVarS S.R. Kim, J. Ramos, R. Vinarao, S. Hechanova, K.K. Jena (Philippines)

36

Poster Area

P0041

MagniTUDe OF HeTerOSiS FOr yieLD anD iTS COMPOnenT TraiTS in HyBriD riCe (Oryza SaTiVa L.) UnDer rainFeD COnDiTiOn B.A. VEERESHA, N.G. HANAMARATTI, P.M. SALIMATH, M.B. CHETTI, B.C. VIRACKTMATH (India)

P0042

a riCe BreeDing PrOgraM FOr WOrLD FOOD SeCUriTy M. Ashikari, H. Yasui, E. Angeles, P. Cuong, A. Yoshimura (Japan)

P0043

STanDarDizaTiOn OF OPTiMUM STage OF aPPLiCaTiOn anD DOSe OF giBBereLLiC aCiD FOr MaXiMizing THe SeeD yieLD POTenTiaL in TnaU riCe HyBriD COrH 3 V. Vakeswaran, V. Palanisamy (India)

P0044

MarKer-aSSiSTeD BreeDing OF QTSn4 FOr inCreaSing grain yieLD OF POPULar inDiCa-TyPe riCe CULTiVarS in SOUTHeaST anD SOUTH aSia D. Fujita, E. Simon, K. Sasaki, R. Gannaban, T. Ishimaru, N. Kobayashi (Japan)

P0045

yieLD TriaL OF TranSgeniC riCe PLanTS OVerPrODUCing Maize PHOSPHOenOLPyrUVaTe CarBOXyLaSe (PePC) A. De Luna, H.C. Lin, S. Karki, S. Covshoff, F. Danila, J. Sagun, R. Libiternos, P. Pablico, J. Dionora, W.P. Quick (Philippines)

P0046

yieLD enHanCeMenT OF inDiCa CULTiVarS USing a gene, SPiKe1 TO inCreaSe TOTaL SPiKeLeT nUMBer Per PaniCLe DeriVeD FrOM TrOPiCaL JaPOniCa CULTiVar N. Kobayashi, K. Sasaki, Y. Fukuta, T. Ishimaru, D. Fujita (Japan)

37





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00


gene regULaTiOn

Poster No

Poster No

P0047

geneTiCS anD MOLeCULar MaPPing OF STigMa eXerTiOn in MainTainer LineS OF HyBriD riCe K. Jayaramulu, K.B. Kemparaju, M. Sheshumadhav, A.S. Hari Prasad, P. Senguttuvel, P. Revathi, R.M. Sundaram, N. Shobha Rani, M.G. Gayathri, N. Sravan Raju, S. Arun Kumar, P. Koteshwar Rao, P.J. Suryendra, B.C. Viraktamath (India)

P0048

iS raDiaTiOn USe eFFiCienCy WOrTH SeLeCTing FOr TO raiSe riCe yieLD POTenTiaL? M.R.C. Laza, U. Kumar, K.V. Mendez, M. Malabayabas, M. Dingkuhn (Philippines)

P0049

QTL MaPPing FOr grain WeigHT USing near-iSOgeniC LineS FrOM an inTerSPeCiFiC CrOSS in riCe S. Yeo, Y.T. Yun, H.H. Kim, S.N. Ahn (Korea)

P0050

FUnCTiOnaL anaLySiS OF a gene aSSOCiaTeD TO yieLD THrOUgH OVer-eXPreSSiOn anD gene SiLenCing M.V. Sapasap, D. Fujita, K.R. Trijatmiko, A. Tagle, N. Tsakirpaloglou, T. Ishimaru, N. Kobayashi, I. Slamet-Loedin (Philippines)

P0051

COMBining aBiLiTy anaLySiS OF THe PHOTOTHerMOSenSiTiVe geniC MaLe STeriLiTy LineS (PTgMS) OF JaPOniCa riCe. H. El-Mowafi, R. Abdallah, E.M.A.N. Fahmy, S. Hassan (Egypt)

38

Poster Area

P0052

gene SiLenCing OF Oryza SaTiVa gLyCine DeCarBOXyLaSe COMPLeX H (OSgDCH) PrOTein By arTiFiCiaL Mirna TO engineer C4 PaTHWay in riCe R. TAPIA, S. Karki, H.C. Lin, S. Covshoff, X. Yin, F. Montecillo, M. Acoba, F. Danila, A.C. De Luna, A. Lazaro, S. Kamal, A. Francisco, J. Reyes, G. Lorenzana, M. Dela Rosa, I. Slamet-Loedin, J. Hibberd, P. Quick (Philippines)

P0053

MOLeCULar anD PHySiOLOgiCaL CHaraCTerizaTiOn OF TranSgeniC inDiCa riCe eXPreSSing Maize C4 geneS, PHOSPHOenOLPyrUVaTe CarBOXyLaSe anD PyrUVaTe OrTHOPHOSPHaTe DiKinaSe F. Danila, R. Coe, S. Karki, H. Lin, S. Covshoff, A. Elmido-Mabilangan, W.P. Quick, M. Dionisio-Sese, M.G. Diaz, L. Cardenas (Philippines)

P0054

iMPrOVeMenT OF SaLiniTy TOLeranCe in riCe: TOWarDS FUnCTiOnaL VaLiDaTiOn OF eUKaryOTiC TranSLaTiOn iniTiaTiOn FaCTOr 4a (eiF-4a) in riCe. B. BHATTACHARJEE, P.S. Reddy, M. Garladinne, M.K. Reddy, R.K. Sarkar, O.N. Singh (India)

P0055

OVereXPreSSiOn OF a SeeD SPeCiFiC CDPK gene OSCPK31 reSULTS in earLy SeeD grain FiLLing anD MaTUriTy in TranSgeniC riCe B. Sena Munuswamy, P. Manimaran, R.M. Sundaram, G. Ravi Kumar, A.K. Tyagi (India)

39





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

gene regULaTiOn (contd.)


Poster No

Poster No

Poster Area

P0056

COMParaTiVe STUDy On CyTOPLaSMiC MaLe STeriLiTyaSSOCiaTeD geneS, OrF79, OrF113, OrF352, DeriVeD FrOM Oryza rUFiPOgOn K. Toriyama, K. Igarashi, M. Okazaki, K. Motomura, T. Kazama (Japan)

P0062

MOri1 iS THe Key FaCTOr OF JUVeniLe-aDULT PHaSe CHange in riCe. Y. Kitomi, N. Tanaka, M. Kojima, H. Sakakibara, K.I. Hibara, J.I. Itoh, Y. Nagato (Japan)

P0057

DeTerMinaTiOn OF 5' anD 3' enDS OF OrF113 rna aSSOCiaTeD WiTH rT98-TyPe CyTOPLaSMiC MaLe STeriLiTy in riCe. K. Igarashi, T. Kazama, K. Motomura, K. Toriyama (Japan)

P0063

geneTiC regULaTiOn OF PHOSPHOrUS HOMeOSTaSiS DUring grain FiLLing in riCe K. Jeong, T.J. Rose, D.L.E. Waters, S. Heuer, G.J. King (Australia)

P0058

DiSSeCTiOn anD VaLiDaTiOn OF TWO QUanTiTaTiVe TraiT LOCi FOr grain WeigHT LinKeD in rePULSiOn On THe LOng arM OF CHrOMOSOMe 1 OF riCe (Oryza SaTiVa L.) J. Ying, H. Zhang, Y. Chen, L. Guo, J. Chen, D. Huang, Y. Zhu, Y. Fan, J. Zhuang (China)

P0064

UBiQUiTin DOMain COnTaining PrOTein inTeraCTS WiTH rF2, a reSTOrer OF FerTiLiTy OF LD-TyPe CyTOPLaSMiC MaLe STeriLiTy in riCe. S. Fujii, T. Kazama, I. Yukihiro, S. Kojima, K. Toriyama (Japan)

P0065

reLaTiOnSHiP BeTWeen TiLLer angLe, graViTOrOPiSM anD STarCH COnTenT in a LOW STeM STarCH riCe MUTanT M. Okamura, T. Hirose, R. Ohsugi, N. Aoki (Japan)

P0066

Maize aLPHa-zein PrOMOTer DriVeS enDOSPerM-SPeCiFiC eXPreSSiOn OF TranSgene in riCe J. Beslin, S. Geetha, K.K. Kumar, E. Kokiladevi, L. Arul, P. Balasubramanian, D. Sudhakar(India)

P0067

a MULTiFUnCTiOnaL riCe gerMin-LiKe PrOTein WiTH a CriTiCaL rOLe in enDOSPerM DeVeLOPMenT N.K. Tsakirpaloglou, M.L. Raorane, R.H. Oane, A. Min, A. Treumann, M. Edwards, L. Conrad, A.M.R. Gatehouse, V. Sundaresan, A. Kohli (Philippines)

P0059

an inDUCeD DOUBLe MUTanT SHOWing SHOOT LiKe SPiKeLeT FrOM LHS (LeaFy HULL STeriLe) TyPe I. Takamure, K. Oshima, K. Fukuroi, A. Nakada, Y. Kishima (Japan)

P0060

riCe PLaSTOCHrOn geneS regULaTe Organ Size anD aCT DOWnSTreaM OF THe giBBereLLin SignaLing PaTHWay M. MIMURA, Y. Nagato, J. Itoh (Japan)

P0061

iDenTiFiCaTiOn OF aDaXiaLizeD LeaF2 gene inVOLVeD in THe PaTTerning OF LeaF, eMBryO anD enDOSPerM THrOUgHOUT PrOPer ePiDerMaL DeVeLOPMenT K. Hibara, J. Itoh, Y. Nagato (Japan)

40

41





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00



Poster No

Poster No

P0068

DiSTinCT PHenOTyPeS OF riCe 'LeaF STarCH eXCeSS' MUTanTS THaT eXHiBiTS HyPeraCCUMULaTiOn OF STarCH in LeaVeS N. Aoki, C. Liang, M. Okamura, A. Miyao, H. Hirochika, T. Terao, T. Yamagishi, R. Ohsugi, T. Li, T. Hirose (Japan)

P0069

riCe TUngrO BaCiLLiFOrM VirUS (rTBV) BaSeD VirUS inDUCeD gene SiLenCing (VigS) in riCe : a FUnCTiOnaL genOMiCS aPPrOaCH R. RAVI KANT, I. INDRANIL DASGUPTA (India)

P0070

SSr VariaTiOn anD iTS eFFeCTS On SeCOnDary STrUCTUre in SignaL TranSDUCTiOn geneS OF MagnaPOrTHe Oryzae L. Liu, C.Y. Li, J. Yang, J. Zhao, Y.L. Du (China)

P0071

aSSeSSMenT OF CanDiDaTe PrOMOTerS FOr CeLL-SPeCiFiC TranSgene eXPreSSiOn in Oryza SaTiVa L. M. Acoba, S. Karki, U. Gowik, F. Montecillo, R. Tapia, J. Reyes, G. Lorenzana, M. Dela Rosa, A. De Luna, I. Slamet-Loedin, J. Hibberd, P. Westhoff, W. Quick (Philippines)

P0072

P0073

MOLeCULar MaPPing OF THe raTOOning FOr OVeryear in Oryza LOngiSTaMinaTa S.L. Zhang, F.Y. Hu (China) geneTiC anaLySiS OF SUgary enDOSPerM in riCe Y. Lee, S.M. Jang, M.S. Choi, R. Piao, E. Koh, H.J. Koh (Korea)

42

Poster Area

P0074

SUrVey OF SeQUenCe POLyMOrPHiSM in TOr PaTHWay geneS aMOng DiFFerenT riCe VarieTieS anD THeir FUnCTiOnaL iMPLiCaTiOn H. Koh, S. Kim (Korea)

P0075

CHaraCTerizaTiOn anD Fine-MaPPing OF an OPen-HULL STeriLe MUTanT in riCe. H. Koh, Y. Yu, D. Lee (Korea)

P0076

gene eXPreSSiOn PrOFiLeS OF gerMinaTing riCe CULTiVarS WiTH eXTreMe VariaTiOn LeVeL OF UnDerWaTer SeeDLing VigOr O. Manangkil, A.L. Baniqued, H.T.T. Vu, N. Mori, C. Nakamura (Philippines)

P0077

iSOLaTiOn anD CHaraCTerizaTiOn OF a DOMinanT DWarF MUTanT, D-H, in riCe R. Piao, H. Koh (Korea)

P0078

Fine MaPPing anD CHaraCTerizaTiOn OF a gianT eMBryO MUTanT, ge-M, in riCe G. Lee, A. Han, S. Jang, H. Koh (Korea)

P0079

CHaraCTerizaTiOnS anD Fine MaPPing OF a MUTanT gene FOr PaniCLe aPiCaL aBOrTiOn in riCe B. Kim, B. Akter, E. Koh, H. Koh (Korea)

43





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00



Poster No

Poster No

P0080

P0081

eaCH ManiPULaTiOn OF TWO aLPHa-enDO-BeTa-1,4gLUCanaSe geneS, aTCeL6 anD gMCeL7, reDUCeS SUSCePTiBiLiTy TO HeTerODera gLyCineS in SOyBean rOOTS M. Woo, H. Beard, M. MacDonald, E. Brewer, R. Youssef, H. Koh, H. Kim, B. Matthews (Korea) rOOT COrTeX FOrMaTiOn inVOLVeS OSSHr1 & OSSHr2, TWO OrTHOLOgS OF THe araBiDOPSiS THaLiana graS TranSCriPTiOn FaCTOr aTSHr S. HENRY, F. DIVOL, G. PAULUZZI, J. PUIG, N. LANAU, S. CESARI, A. DIEVART, D. MIEULET, E. GUIDERDONI, C. PERIN (France)

P0082

OVereXPreSSiOn OF THe Maize C4 PHOSPHOenOLPyrUVaTe CarBOXyLaSe (PePC) gene in riCe A. Francisco, S. Covshoff, F. Montecillo, R. Tapia, M.G. Acoba, F. Danila, J.V. Sagun, R. Libiternos, A.C. De Luna, M. Dionora, H.C. Lin, I. Slamet-Loedin, J. Hibberd, P. Quick (Philippines)

P0083

geneTiC anaLySiS OF a SHOrT grain MUTanT in riCe S. Shuhei, K. Izumi, A. Tsuyu, Y. Masahiro, K. Hidemi, M. Kotaro, I. Yukimoto (Japan)

P0084

eXPreSSiOn OF irOn HOMeOSTaSiS-reLaTeD geneS in an irOn CHeLaTOr anD STOrage OVereXPreSSOr Line iS MODULaTeD TO COinCiDe MainLy WiTH THe raPiD MiLKy enDOSPerM DeVeLOPMenT STage P. FRANCISCO, C. Duenas, R.K. Tridjatmiko, G. Barry, P. ChadhaMohanty, I.H. Islamet-Loedin (Philippines)

44

Poster Area

P0085

MOLeCULar BeaCOn PrOBe BaSeD PrOMOTer MOTiF DeTeCTiOn & in SiLiCO PrOTein-Dna inTeraCTiOn STUDieS DUring SUBMergenCe in riCe (Oryza SaTiVa L.) G.K. Prajapati, A. Kumar, D.M. Pandey (India)

P0086

geneTiC MODiFiCaTiOn Via TaLen TeCHnOLOgy TO CreaTe SiTe-SPeCiFiC MUTaTiOnS in riCe X. Yin, A. Macovei, T. Cermak, C. Cantos, M. Sapasap, W. Quick, D. Voytas, P. Chadha-Mohanty, I. Slamet-Loedin (Philippines)

P0087

genOMe-WiDe DiSTriBUTiOn OF QUanTiTaTiVe TraiT LOCi (QTL) FOr HigH grain irOn anD zinC in riCe A. Amparado, C. Manito, M.A. Asilo, F. Tesoro, J. Rey, G. Barry, P. Virk, B.P. M Swamy (Philippines)

45





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area

aBiOTiC STreSS TOLeranCe

aBiOTiC STreSS TOLeranCe (contd.)

Poster No MaPPing anD CHaraCTerizaTiOn OF QLTg11, a QTL P0088 COnTrOLLing LOW TeMPeraTUre gerMinaBiLiTy On CHrOMOSOMe 11 in riCe (Oryza SaTiVa L.) N. Iwata, K. Fujino (Japan)

Poster No eFFeCT OF POLyaMineS On PHySiOLOgy anD SPS1 gene P0094 eXPreSSiOn in riCe (Oryza SaTiVa L. CV. KDML105) UnDer DrOUgHT STreSS. M. Monthatong, W. Pattanagul, J. Pholtaisong (Thailand)

P0089

TOLeranCe OF DiFFerenT riCe VarieTieS TO SOiL SaLiniTy in THe COaSTaL area OF THe THUa THien HUe PrOVinCe, CenTraL VieTnaM T. Hoang, D. Do, M. Oost, J. Dufey (Vietnam)

P0090

MaPPing QTLS FOr TOLeranCe TO FLOODing DUring gerMinaTiOn anD earLy SeeDLing grOWTH in riCe (Oryza SaTiVa L.) USing ir64 ? TKM9 POPULaTiOn. O. Myint, A. Ismail, J. Hernandez, E. Septiningsih, P. Cruz (Myanmar (Burma))

P0091

inTegraTing MarKer-aSSiSTeD SeLeCTiOn inTO THe COnVenTiOnaL BreeDing TO iMPrOVe SaLT TOLeranCe OF riCe (Oryza SaTiVa L.) L. NGUYEN THI (Vietnam)

P0092

CUrrenT STaTUS OF HeaT TOLeranCe riCe BreeDing in THe PHiLiPPineS N. Manigbas, L.B. Madrid, C.C. Cardenas (Philippines)

P0093

reSPOnSe OF riCe VarieTieS TO HeaT STreSS UnDer aerOBiC anD FLOODeD COnDiTiOnS in THe VegeTaTiVe STage L. Le, J.M. Mitchel, F.K. Fukai Shu (Vietnam)

46

P0095

DrOUgHT TOLeranCe eVaLUaTiOn OF riCe UnDer DiFFerenT graDienT anD QUanTiTaTiVe WaTer COnTrOL H. Kang, D. Luo, K. Chen, J. Kang, G. Ren (China)

P0096

riCe PLaSMa MeMBrane inTrinSiC PrOTeinS are inVOLVeD in TranSPOrT anD PrOViDing TOLeranCe TO BOrOn TOXiCiTy K. Kumar, K.A. Mosa, S. Chhikara, O. Dhankher (India)

P0097

MULTi-enVirOnMenT TriaLS (MeTS) OF riCe BreeDing LineS FOr LOCaTiOn SPeCiFiC aDaPTaTiOn anD aCCePTaBiLiTy in rainFeD LOWLanD riCe eCOSySTeM (2012DS-2012WS) T. Sigari, N. Desamero, M.A.R. Orbase, M.C. De Peralta, N.N. Baliuag, E.S. Ladia, C.P. Ubales, V. Agreda, T.F. Padolina (Philippines)

P0098

eXPreSSiOn anD PrOMOTer anaLySiS OF SiX STreSSinDUCiBLe geneS in riCe W. RERKSIRI, Z. Xianwen, C. Xinbo (China)

P0099

BreeDing FOr HeaT anD DrOUgHT TOLeranT riCe in arizOna P. Sanchez (USA)

47





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No HaPLOTyPe DiVerSiTy OF SOMe neW SUBMergenCe P0100 TOLeranT riCe gerMPLaSM anD inTrOgreSSiOn OF SUB1 QTL inTO THree rainFeD LOWLanD riCe VarieTieS OF BangLaDeSH K. Iftekharuddaula, S. Ghosal, E. Hoque, J. Ferdous, R. Yasmeen, Z.R. Moni, E.M. Septiningsih (Bangladesh)

Poster No inFLUenCe OF WaTer STreSS enVirOnMenT On VariOUS P0105 PLanT TraiTS OF riCe (Oryza SaTiVa L.) M. Sabar (Pakistan)

P0101

rOLeS OF KaSaLaTH aLLeLe regULaTing THe PLaSTiCiTy OF LaTeraL rOOT DeVeLOPMenT in THe grOWTH OF riCe PLanTS UnDer FLUCTUaTing SOiL MOiSTUre STreSS J. Niones, R. SURALTA, Y. INUKAI, M. Nakata-Kano, A. YAMAUCHI (Philippines)

P0102

aLLeLiC DiVerSiTy SUrVey in riCe gerMPLaSM FOr LOW TeMPeraTUre TOLeranCe: a USeFUL TOOL FOr MarKeraSSiSTeD BreeDing N. Zenna, M. Ndomondo, R. Kwayu, B. Wanjala, J. Harvey (Tanzania)

P0103

raDiCaL-inDUCeD CeLL DeaTH1 (rCD1): a PrOMiSing gene TO enHanCe aBiOTiC STreSS TOLeranCe in Oryza SaTiVa (riCe). S. Qamarunnisa, B. Siddiqua, A. Azhar (Pakistan)

P0104

STUDieS On riCe iMPrOVeMenT FOr LOWer SenSiTiViTy TO COLD STreSS M. Salam, N.K. Saha, P.S. Saha, M.M. Islam, M.R. Karim (Bangladesh)

48

P0106

SUSTainaBLe riCe SeeD PrODUCTiOn anD DeLiVery SySTeM FOr SOUTHern BangLaDeSH L. HASSAN (Bangladesh)

P0107

UTiLiTy OF PUP 1 gene BaSeD MarKerS FOr THe VarieTaL SCreening OF riCe(Oryza SaTiVa) FOr PHOSPHOrUS DeFiCienCy TOLeranCe N. Kottearachchi, S. Wijesekara (Sri Lanka)

P0108

genOTyPe By enVirOnMenT (gXe) inTeraCTiOn anD STaBiLiTy aSSeSSMenT OF riCe genOTyPeS FOr SaLT TOLeranCe USing aMMi BiPLOT anaLySiS S.L. Krishnamurthy, S.K. Sharma, D.K. Sharma, Y.P. Singh, V.K. Mishra, D. Burman, B. Maji, R.K. Gautam, K.K. Manohara, B.C. Marandi, D.P. Singh, G. Padmavathi, K.D. Patil, S. Thirumani, O.P. Veram, A.H. Khan, S. Tiwari, M. Shakila, G.B. Gregorio, R.K. Singh (India)

P0109

anaLyzing CanDiDaTe geneS anD aSSOCiaTiOn MaPPing FOr SeeDLing STage SaLiniTy TOLeranCe in riCe (Oryza SaTiVa) S. Thein, G.B. Gregorio, R.K. Singh, M. Thomson, M. Diaz, J.E. Hernandez (Myanmar (Burma)

49





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No DrOUgHT TOLeranCe OF SeLeCTeD PHiLiPPine TraDiTiOnaL P0110 riCe gerMPLaSM M. Ferrer, T. Borromeo, R. Suralta (Philippines)

Poster No MaPPing THe SaLiniTy TOLeranCe QTL in riCe (Oryza SaTiVa P0116 L.) VarieTy HaSaWi aT SeeDLing STage USing F6 reCOMBinanT inBreD LineS POPULaTiOn E.D. Pascual, C.B. Dela Viña, M.S. Mendioro, J.E. Hernandez, J.C. Amas, A.D. Barretto, A.G.C. Sajise, G.B. Gregorio (Philippines)

P0111

P0112

P0113

geneTiC COMPOnenTS anD QTL anaLySiS OF SaLiniTy TOLeranCe aT THe rePrODUCTiVe STage in riCe (Oryza SaTiVa L.) R. Mohammadi, M. Mendioro, G. Diaz, A.G. Sajise, M. Arceta, A.N. Vispo, J.C. Amas, A. Barretto, G. Gregorio, R.K. Singh (Iran)

P0117

DeVeLOPMenT OF DrOUgHT TOLeranT TranSgeniC riCe By STreSS inDUCiBLe TranSCriPTiOn FaCTOr aTDreB1a in eLiTe inDiCa CV. BPT5204 B. Sena Munuswamy, G. Ravi Kumar, P. Manimaran, R.M. Sundaram, S.R. Voleti, D. Subrahmanyam (India)

OVereXPreSSiOn OF gLUTaMine SynTHeTaSe gene iMPrOVeS TOLeranCe TO CaDMiUM STreSS in TranSgeniC riCe H.J. Lee, S.E. Abdula, D.W. Jang, M.G. Jee, S.H. Park, U.H. Yoon, K.K. Kang, I.S. Nou, Y.G. Cho (Korea)

P0118

iMPrOVeMenT OF SUBMergenCe TOLeranCe in LOCaL riCe Line Bg 96-741 THrOUgH BaCK CrOSS BreeDing; aSSiSTeD By MOrPHOLOgiCaL anD MOLeCULar SCreening G. Samarasinghe, W.D.I.P. Wimalasena, H. Ratnayake, D.S. Kakulandara, E.L.D.A. Siriwardena (Sri Lanka)

BrUge1 enCODing UDP-gLUCOSe-4-ePiMeraSe FrOM BraSSiCa raPa May iMPrOVe DrOUgHT TOLeranCe anD yieLD in TranSgeniC riCe H.J. Lee, S.E. Abdula, D.W. Jang, M.C. Nino, F.C. Nogoy, M.S. Kim, I.S. Nou, K.K. Kang, Y.G. Cho (Korea)

P0119

MaPPing COnSiSTenT Large eFFeCT QTLS FOr yieLD UnDer DrOUgHT in TargeT POPULaTiOnS OF enVirOnMenT USing LOCaLLy aDaPTeD inDiCa riCe (Oryza SaTiVa L.) CULTiVarS C. Ranganathan, S. Jebakumar Prince, A. Beena (India) MaPPing OF nOVeL PHOSPHOrUS eFFiCienCy TraiTS anD LOCi THrOUgH genOMe-WiDe aSSOCiaTiOn anaLySiS M. Wissuwa, T. Fukuda, A. Mori, M.T. Rose, J. Pariasca-Tanaka, T.J. Rose (Japan)

P0114

a SiMPLe, eFFeCTiVe SCreening PrOCeDUre FOr DrOUgHT TOLeranT riCe VarieTy D. Shin, S. Park, U. Hwang, J. Lee, S. Han, M. Nam, D. Pakr (Korea)

P0115

SCreening anD iDenTiFiCaTiOn OF P DeFiCienCy TOLeranT riCe genOTyPeS USing PUP1 SPeCiFiC MarKerS S. Banerjee, S. Verulkar, M. Dubey, A. Prasad (India)

50

P0120

P0121

STrUCTUre, FUnCTiOn anD reSPOnSe OF inDiCa riCe anneXinS in geneTiCaLLy DiVerSe genOTyPeS UnDer DrOUgHT STreSS S. Barthakur, R. Kumar, S. Deka, N. Pandey (India)

51





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No geneTiC DiSSeCTiOn On aerOBiC aDaPTaTiOn OF UPLanD P0122 riCe P. Xu, J. Zhou, J. Li, Y. Zhang, H. Li, F. Yang, D. Tao (China)

Poster No CHaraCTerizaTiOn OF ag1, a MaJOr QTL FOr TOLeranCe OF P0128 anaerOBiC COnDiTiOnS DUring gerMinaTiOn FOr DireCTSeeDeD riCe SySTeMS M. Pelayo, T. Kretzschmar, L. Gabunada, A. Ismail, D. Mackill, E. Septiningsih (Philippines) SHOOT eLOngaTiOn anD TiLLering aBiLiTy are eSSenTiaL P0129 FOr COnFerring TOLeranCe FOr STagnanT FLOODing in riCe F. Entila, E. Ella, I. Abdelbagi (Philippines)

P0123

TOWarDS iMPrOVeD SaLiniTy TOLeranCe OF riCe THrOUgH aLTeraTiOn OF SODiUM TranSPOrT anD HigH THrOUgHPUT iMage BaSeD PHenOTyPing A. Hairmansis, B. Berger, M. Tester, S. Roy (Australia)

P0124

QTL FOr iMPrOVing HeaT TOLeranCe in riCe C. Ye, F.A. Tenorio, H.W. Thein, Y. Yu, E.D. Redoña, S.V.K. Jagadish (Philippines)

P0125

STUDy On THe SUBMergenCe TOLeranCe CHaraCTeriSTiCS On nePaLeSe riCe gene POOL S. Pantha, S. Khatiwada (Nepal)

P0126

P0127

THe inFLUenCe OF SOWing DenSiTy anD TreaTMenT WiTH TrineXaPaC-eTHyL On riCe (Oryza SaTiVa L.) SOMe agrOnOMiC anD QUaLiTy CHaraCTeriSTiCS. R. Unan (Turkey) DrO2: a QUanTiTaTiVe TraiT LOCUS COnTrOLLing DeeP rOOTing On CHrOMOSOMe 4 DeTeCTeD in THree riCe MaPPing POPULaTiOnS Y. Uga, E. Yamamoto, N. Kanno, S. Kawai, T. Mizubayashi, S. Fukuoka (Japan)

52

P0130

DeVeLOPMenT OF HeaT TOLeranT riCe VarieTieS THrOUgH gerMPLaSM SCreening anD MOLeCULar MaPPing M. Kumaraswamy, Motilal, S. Srikanth, P. Raghuveer Rao, S.R. Voleti, P. Seguttuvel, L.V. Subbarao, B.C. Viraktamath (India)

P0131

generaTiOn Mean anaLySiS OF DrOUgHT TOLeranCe in neW generaTiOnS OF inTerSPeCiFiC riCe genOTyPeS J. Lamo, T. Pangirayi, J. Derera, P. Okori (Uganda)

P0132

annOSi2, a MULTiFUnCTiOnaL anneXin gene inVOLVeD in aBiOTiC anD BiOTiC STreSS in riCe S. Deka (India)

P0133

geneTiC VariaTiOn OF TOLeranCe FOr irOn TOXiCiTy USing agar nUTrienT SOLUTiOn in riCe A. Tomita, J. Pariasca Tanaka, M. Wissuwa, Y. Fukuta (Japan)

53





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No WHOLe genOMe aSSOCiaTiOn MaPPing FOr rOOT P0134 MOrPHOLOgiCaL anD anaTOMiCaL TraiTS in a JaPOniCa PaneL UnDer niTrOgen DeFiCienCy E. THOMAS, J. SOEWARTO, A. DARDOU, A. AUDEBERT, A. DIEVART, C. PERIN, E. GUIDERDONI, B. COURTOIS (France)

Poster No reSTOring anD enHanCing riCe PrODUCTiViTy OF COaSTaL P0140 SaLine areaS OF anDaMan & niCOBar iSLanDS THrOUgH FARMERS’ PARTICIPATORY VARIETY SELECTION R.K. Gautam, P.K. Singh, S.K. Zamir Ahmed, A.K. Singh, N. Kumar, A. Velmurugan, S. Dam Roy, R.K. Singh, G.B. Gregorio, A.M. Ismail (India)

P0135

DeVeLOPMenT OF riCe HyBriDS WiTH aBiOTiC STreSS TOLeranCe U. Prasadarao, S. Satishreddy, C. Ramakrishna, P. Satishkumar (India)

P0141

QTLS aSSOCiaTeD WiTH anaerOBiC gerMinaTiOn OF riCe(Oryza SaTiVa) S. Hsu, C. Tung (Taiwan)

P0136

a genOMe WiDe aSSOCiaTiOn STUDy (gWaS) TO iDenTiFy geneS aSSOCiaTeD WiTH TOLeranCe TO irOn TOXiCiTy in riCe (Oryza SaTiVa L.) E. Matthus, L.B. Wu, G.B. Gregorio, M. Becker, M. Frei (Germany)

P0142

iDenTiFiCaTiOn OF nOVeL QTLS aSSOCiaTeD WiTH SaLiniTy TOLeranCe in riCe aT SeeDLing STage USing CHiKiraM PaTnai/azUCena CrOSS M. Sarker, M.A. Newaz, M.P. De Ocampo, M.J. Thomson, A.M. Ismail (Bangladesh)

P0137

THe riCe OSPSTOL1 PrOTein KinaSe in THe PUP1 MaJOr QTL COnFerS TOLeranCe OF PHOSPHOrUS DeFiCienCy R. Gamuyao, J.H. Chin, J. Pariasca-Tanaka, P. Pesaresi, S. Catausan, C. Dalid, I. Slamet-Loedin, E. Tecson-Mendoza, M. Wissuwa, S. Heuer (Philippines)

P0143

grain yieLD QTL WiTH COnSiSTenT eFFeCT UnDer rePrODUCTiVe DrOUgHT STreSS in riCe A. Palanog, B.P.M. Swamy, N. Shamsudin, S. Dixit, J.E. Hernandez, T.H. Borromeo, P.C. Sta. Cruz, A. Kumar (Philippines)

P0138

Fine MaPPing OF QTL FOr HeaT TOLeranCe OF riCe On CHrOMOSOMe 1 H. Thein, C. Ye, F. Tenorio, Y.O.Y.E. Yu, E. Redona (Philippines)

P0144

PrOMiSing riCe LanDraCeS FOr SUBMergenCe TOLeranCe P. Singh, A. Singh, S. Eram, R. Khare, A.K. Singh, N.K. Singh (India)

P0139

riCe HeaT TOLeranCe UnDer DiFFerenT reLaTiVe HUMiDiTy DUring anTHeSiS F. Tenorio, C. Ye, H. Thein, Y. Yu, E. Redoña (Philippines)

P0145

eVaLUaTiOn OF HeaT anD COLD TOLeranCe OF riCe VarieTieS aT BOOTing anD FLOWering STageS Y. Yu, F. Tenorio, S.V. Jagadish, E. Redoña, C. Ye (Philippines)

54

55





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No iDenTiFiCaTiOn OF CanDiDaTe geneS UnDerLying a QTL P0146 CLUSTer gOVerning DrOUgHT TOLeranT TraiTS eMPLOying rna-SeQ aPPrOaCH in riCe S. Patil, R. Ronanki, D.A.K. Deborah, S.S. Jena, A. Srividhya, E.A. Siddiq, L.R. Vemireddy(India)

Poster No enHanCeD rOOT MOrPHOLOgy OF riCe THrOUgH BiParTiTe P0153 inTeraCTiOn inVOLVing TriCHODerMa SPP. MODiFying BiOCHeMiCaL PaTHWayS inFLUenCing aDaPTaTiOn OF PLanTS TO aBiOTiC STreSS T. Agrawal, A. Kotasthane, U.S. Singh (India)

P0147

BreeDing riCe FOr SaLT TOLeranCe in niger O. SOULEYMANE (Niger)

P0148

LOW-TeMPeraTUre SenSiTiViTy anD POLLen STeriLiTy are CHARACTERIZED THE ANTHER TRANSCRIPTOME’S REPEAT SeQUenCeS S. Ishiguro, K. Ogasawara, K. Fujino, Y. Sato, Y. Kishima (Japan)

P0149

QUanTiTaTiVe TraiT LOCi anaLySeS OF rOOT PLaSTiC DeVeLOPMenTaL reSPOnSeS TO PrOgreSSiVe DrOUgHT STreSS in riCe R.R. Suralta, N.B. Lucob, L.M. Perez, J.M. Niones (Philippines)

P0150

POLyMOrPHiSM OF rUSSian riCe VarieTieS On LOCUSeS DeTerMining aDaPTaBiLiTy TO STreSS. E. Kharitonov, J. Goncharova (Russia)

P0151

QTLS MaPPing OF PHySiOLOgiCaL TraiT reLaTeD TO SaLT TOLeranCe in riCe C. Lee, C.W. Tung (Taiwan)

P0152

genOMe-WiDe aSSOCiaTiOn anaLySiS FOr HeaT TOLeranCe aT FLOWering DeTeCTeD a Large SeT OF geneS inVOLVeD in aDaPTaTiOn TO THerMaL anD OTHer STreSSeS T. Lafarge, C.S. Bueno, B. Courtois, N. Ahmadi (France)

56

P0154

MarKer aSSiSTeD BaCKCrOSS BreeDing FOr inTrOgreSSiOn OF grain yieLD UnDer DrOUgHT STreSS QTLS inTO THe eLiTe SUSCePTiBLe riCe VarieTy ir64 SUB1 N. Mandal, A. Anupam, S.M. Quatadah, M. Variar, A. Henry, A. Kumar (India)

P0155

iSOLaTiOn anD FUnCTiOnaL anaLySiS OF BrUgT enCODing a UDP-gLyCOSyLTranSFeraSe FrOM BraSSiCa raPa in TranSgeniC riCe M.S. Kim, H.J. Lee, D.W. Jang, M.C. Nino, D.A. YU, K.K. Kang, I.S. Nou, Y. Cho (Korea)

P0156

eXPreSSiOn STUDieS OF SUB1 geneS in riCe HyBriDS COMPareD WiTH THeir ParenTS, DiFFering in TOLeranCe TO SUBMergenCe A. Masuduzzaman, D.R. E. Septingsih, M.R.S. D. Sanchez, M.R. A. Pamplona, D.R. S. Heurer, D.R. A. Ismail, D.R. H. U. Ahmed, D.R. D. J. Mackill (Bangladesh)

P0157

QTL MaPPing OF SaLiniTy TOLeranCe TraiTS reLaTeD TO SeeDLing rOOT grOWTH S. Lin, C.W. Tung (Taiwan)

57





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13:30 - 15:00

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13:30 - 15:00

Poster Area



Poster No COnFirMaTiOn OF a MaJOr QTL: SaLTOL in riCe FOr SaLT P0158 TOLeranCe By SeLeCTiVe genOTyPing S. Thirumeni, J. Karthick, T. Somasekar, K. Paramasivam, S. Nadaradjan, V. Vengadessan (India)

Poster No aSSOCiaTiOn MaPPing TO iDenTiFy genOMiC regiOnS P0163 gOVerning VariaBiLiTy in rOOT TraiTS anD WUe in riCe (Oryza SaTiVa.L). B.R. Raju, M.V. Mohankumar, M.S. Ratnakar, H.B. Diwakar, K. Sumanthkumar, M.P. Rajanna, M.S. Sheshshayee (India)

P0159

COMBaTing aBiOTiC STreSSeS: MarKer-aSSiSTeD PyraMiDing OF DTy QTLS PrOViDeS an eFFiCienT aLTernaTiVe A. Singh, S. Dixit, M.T. Sta. Cruz, P.T. Maturan, J.R. Ramirez, M.P. Catolos, J.C. Ontoy, A. Kumar (Philippines)

P0160

P0164

PHenOTyPing anD iDenTiFiCaTiOn OF MiCrOSaTeLLiTe MarKerS aSSOCiaTeD WiTH FOr rOOT TraiTS anD WaTer USe eFFiCienCy in riCe (Oryza SaTiVa L.) B. RADHA, P. Kumar, V. Vighneswaran (India)

FarMerS' ParTiCiPaTOry VarieTaL SeLeCTiOn: a SUSTainaBLe CrOP iMPrOVeMenT aPPrOaCH FOr 21ST CenTUry Y. Yash Pal Singh, A. Amresh Kumar Nayak, R. Raj Kumar gautam, R. Rakesh Kumar Singh (India)

P0165

OVereXPreSSiOn OF OSnaM12.1 gene aFFeCTS rOOT anD SPiKeLeT BranCHing in riCe (Oryza SaTiVa L.) UnDer DrOUgHT COnDiTiOnS A. Aye Min, R.H. Onae, A.K. Biswals, M. Roanae, A. Kumar, A. Henry, A. Kohli (Myanmar (Burma)

P0161

TWO FUnCTiOnaL geneS aSSOCiaTeD WiTH COLD TOLeranCe aT SeeDLing STage in riCe iDenTiFieD By inTegraTing genOMe-WiDe DiFFerenTiaL eXPreSSiOn WiTH QTL MaPPing J. Zhao, S. Zhang, T. Yang, Q. Liu, X. Wang, B.I.N. Liu (China)

P0166

ParTiCiPaTOry VarieTaL SeLeCTiOn: TOWarDS inCreaSeD RICE PRODUCTIVITY IN MYANMAR’S AYEYARWADDY DELTA G. Gregorio, R. Labios, R.K. Singh, M. Casimero, A.A. Thant, M. Win, M.S. Tun, T. Myint, O. Myint, G. Singleton, D. Johnson (Philippines)

P0162

SSSLS-BaSeD iDenTiFiCaTiOn anD PyraMiDing OF QTLS FOr COLD TOLeranCe aT DiFFerenT DeVeLOPMenTaL STageS T. Yang, S. Zhang, Z. Huang, J. Zhao, Q. Liu, X. Wang, G. Zhang, B. Liu (China)

P0167

L44 - a nOVeL SOUrCe OF HeaT TOLeranCe in riCe R. BAHUGUNA, D. SHAH, J. JHA, M. PAL, C. QUINONES, C. SOLIS, S.V.K. JAGADISH (Philippines) FUnCTiOnaL CHaraCTerizaTiOn OF CanDiDaTe HSPS TO reDUCe HeaT anD DrOUgHT inDUCeD SPiKeLeT STeriLiTy in riCe N. Mattes, R.J. Melgar, R. Malo, I.H. Slamet-Loedin, T. Kretzschmar, S.V.K. Jagadish (Philippines)

58

P0168

59





POSTer SeSSiOn

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13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No QTL iDenTiFiCaTiOn FOr yieLD-reLaTeD TraiTS UnDer P0169 DiFFerenT PHOSPHOrUS anD WaTer COnDiTiOnS FrOM inDiCa X JaPOniCa reCOMBinanT LineS in riCe I.P. Navea, C. Zamora, H.J. Koh, S.H. Kim, K. Titong, J. Chin (Philippines)

Poster No DeVeLOPMenT OF HigH yieLDing THerMO TOLeranT riCe P0175 VarieTieS R. Pottepalem, P. Sudhakar, P. Latha, M. Sreevalli Devi (India) P0176

eXPLOring genOTyPiC VariaTiOn FOr iMPrOVing niTrOgen USe eFFiCienCy in irrigaTeD riCe S. KUCHI, N. C N, S. B., S. I., V. P, V. S. R., K. R. M., S. N., R.A.O. K. V., V. B. C. (India)

P0177

QTL MaPPing FOr TOLeranCe OF anaerOBiC gerMinaTiOn FrOM ir64 anD nanHi USing SingLe nUCLeOTiDe POLyMOrPHiSM (SnPS) M. Baltazar, J.I. Ignacio, M.J. Thomson, A.M. Ismail, M.S. Mendioro, E.M. Septiningsih (Philippines)

P0170

STUDy OF COLD TOLeranCe in TeMPeraTe riCe aT SeeDLing STage G. Donoso, M. Paredes, V. Becerra, M. Leyton, P. Cabas (Chile)

P0171

COLD TOLeranT CyTOPLaSMiC MaLe STeriLe LineS FOr DeVeLOPMenT OF HyBriD riCe (Oryza SaTiVa L.) SUiTaBLe FOr TeMPeraTe HigHLanDS G. Parray, S. Wani, N. Sofi, A. Shikari, A. Hussaini, G. Sanghera (India)

P0172

reSPOnSe OF riCe HyBriDS UnDer WaTer LiMiTeD enVirOnMenTS: a STUDy On yieLD anD MOrPHO PHySiOLOgiCaL TraiTS in riCe M. Devarajan, D. Malarvizhi, C. Vijayalakshmi, S. Manonmani, K. Thiyagarajan (India)

P0178

DiFFerenT TyPeS OF SaLT TOLeranT MeCHaniSMS in riCe aS reVeaLeD By THe DynaMiCS OF na anD K iOn TranSPOrT in reSPOnSe TO SaLT STreSS P. Senadheera, T.L.S. Tirimanne, R.K. Singh, F.J. Maathuis, D. Abeysiriwardena (Sri Lanka)

P0173

HeaT-reLaTeD MOrPHOLOgiCaL VariaTiOnS in riCe VarieTieS Y. Lee, C.M. Lee, S. Kim, H.J. Koh (Korea)

P0179

CanDiDaTe gene anaLySiS OF QTL FOr MeSOCOTyL eLOngaTiOn H.S. Lee, J.W. Kang, D.M. Kim, T. Sato, S.N. Ahn (Korea)

P0174

VaLiDaTiOn OF DiFFerenTiaLLy eXPreSSeD CanDiDaTe geneS FOr SaLiniTy TOLeranCe in inTrOgreSSiOn LineS DeriVeD FrOM THe CrOSS KMr3 X O. rUFiPOgOn S. Mesapogu, G. Pushpalatha, S. Kadiri, A. Prasad Babu, T. Ram, L.V. Subba Rao, D. Subrahmanyam, Brajendra, N. Sarla, V. Rai (India)

P0180

SaLiniTy TOLeranCe QTL MaPPing aT SeeDLing anD rePrODUCTiVe STageS OF riCe M.L.S. Jubay, G.B. Gregorio, A.G.C. Sajise, J.C. Amas, A.D. Barretto, R.Y. Acil, M.G.Q. Diaz, M.S. Mendioro, J.E. Hernandez (Philippines)

60

61





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No SeLeCTiOn OF irOn TOXiCiTy TOLeranCe riCe (Oryza SaTiVa P0181 L.) PLanTS By USing a HyDrOPOniCS MeTHOD anD MiCrOSaTeLLiTe MarKerS Y.A.P.K. Dayasena, W.L.G. Samarasinghe, G.D.A. Priyantha, B.G.D.S. Weerasinghe, K.A.C. Rasanjali, A.A.Y. Amarasinghe (Sri Lanka)

Poster No iDenTiFiCaTiOn OF DrOUgHT-reSPOnSiVe MiCrOrnaS in LeaF P0187 anD STeM TiSSUeS OF Oryza SaTiVa SeeDLingS K. Nadarajah, W. Ratnam, B. Cheah (Malaysia) P0188

SCreening FOr geneTiC reSOUrCeS OF earLy-MOrning FLOWering TraiT in aLien inTrOgreSSiOn LineS DeriVeD FrOM Oryza SPeCieS R. Gannaban, H. Hirabayashi, K. Sasaki, K.K. Jena, T. Ishimaru, T. Ishimaru (Philippines)

P0189

eVaLUaTiOn OF grain yieLD anD yieLD-reLaTeD TraiTS OF near-iSOgeniC LineS FOr DayS TO HeaDing WiTH THe geneTiC BaCKgrOUnD OF an inDiCa-TyPe riCe VarieTy ir64 K. Sasaki, D. Fujita, R. Gannaban, P. Lumanglas, Y. Fukuta, N. Kobayashi, T. Ishimaru (Philippines)

P0182

geneTiC DiVerSiTy anaLySiS OF nOrTH-inDian riCe gerMPLaSM reVeaLeD FOUr SUB-POPULaTiOnS A. Singh, V. Rai, H.S. Balyan, N.K. Singh, E. Septiningsih (India)

P0183

MOrPHO-agrOnOMiC anD MOLeCULar DiVerSiTy OF SaLTTOLeranT PHiLiPPine TraDiTiOnaL riCe VarieTieS R.A.G. Saludares, J.M. Jamago, G.B. Gregorio (Philippines)

P0184

MaPPing QTLS FOr HeaT TOLeranCe reLaTeD TraiTS in MaDHUKar X SWarna riLS V. Vishnu Prasanth, D.V.N. Chakravarthi, K. Anuradha, Y. Venkateswara Rao, M. Suchandranath Babu, V.G.N. Tripura Venkata, S.R. Voleti, S. Neelamraju (India)

P0190

eXPreSSiOn OF SaLiniTy aSSOCiaTeD geneS in DrOUgHT TOLeranT anD SUSCePTiBLe inTrOgreSSiOn LineS DeriVeD FrOM THe CrOSS SWarna X O. niVara S. Mesapogu, S. Kadiri, G. Pushpalatha, A. Prasad Babu, T. Ram, L.V. Subba Rao, D. Subrahmanyam, Brajendra, V. Rai, N. Sarla (India)

iDenTiFiCaTiOn OF a QTL COnTrOLLing earLy HeaDing On a BaCKCrOSSeD inBreD Line DeriVeD FrOM a CrOSS BeTWeen CarreOn anD inDiCa riCe VarieTy, ir64 E. Simon, D. Fujita, R. Gannaban, K. Sasaki, N. Kobayashi, T. Ishimaru, T. Ishimaru (Philippines)

P0191

HigH yieLDing STaBLe genOTyPe FOr rainFeD LOWLanD COnDiTiOn in KarnaTaKa, inDia P. SURENDRA, N.G. HANAMARATTI, M.T. SHARMA, S. KARIKATTI, S. ANURAJ (India) reSPOnSe OF riCe genOTyPeS TO DiFFerenT LeVeLS OF MOiSTUre STreSS anD eSTiMaTiOn OF DrOUgHT inDiCeS A. MS, N. Mandal, M. Variar, A. Henry (India)

P0185

P0186

SaLiniTy TOLeranCe QTLS aT rePrODUCTiVe STage in riCe (Oryza SaTiVa L. CV POKKaLi) J. Domingo, J.E. Hernandez, P.C. Sta Cruz, A.G.C. Sajise, M.V. Arceta, G.B. Gregorio (Philippines)

62

P0192

63





POSTer SeSSiOn

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13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No QUanTiTaTiVe TraiT LOCi anaLySiS OF LODging reSiSTanCe P0193 in a riCe reCOMBinanT inBreD Line POPULaTiOn in irrigaTeD LOWLanDS E.A. Barlaan, H. Sato, H. Hirabayashi, R. Ikeda, T. Imbe (Philippines)

Poster No HaPLOTyPe DiVerSiTy in THe 'SaLTOL' regiOn anD iTS P0198 aSSOCiaTiOn WiTH SeeDLing STage SaLT TOLeranCe in DiVerSe riCe gerMPLaSM B.N. Naresh, S. Gopala Krishnan, P.K. Bhowmick, T. Vanaja, K.K. Vinod, M. Nagarajan, N.K. Singh, K.V. Prabhu, A.K. Singh (India)

P0194

DeVeLOPMenT OF SaLT TOLeranT VerSiOn OF Br11 anD Brri DHan28 THrOUgH MarKer-aSSiSTeD BaCKCrOSSing M.S. Rahman, S. Rahman, T. Haque, M.R. Islam, M.A. Salam, M.J. Thomson, A.M. Ismail, Z.I. Seraj (Bangladesh)

P0195

QTL MaPPing FOr grain yieLD anD yieLD reLaTeD TraiTS UnDer zn DeFiCienT anD nOrMaL FieLD COnDiTiOnS G.Z. Doblas, M.S. Mendioro, M.G.Q. Diaz, R.P. Laude, I.F. Dalmacio, G.V. Vergara, A.G.C. Sajise, M.R. Islam, G.B. Gregorio (Philippines)

P0196

SeLeCTiOn CriTeria anD PHenOTyPiC STaBiLiTy OF MaJOr yieLD COnTriBUTing TraiTS in HeaT TOLeranCe riCe (Oryza SaTiVa L.) V. Yadav, S. Soni, V. Bhadana (India)

P0197

PerUSaL On SeLeCTiOn ParaMeTerS anD nOnHierarCHiCaL eUCLiDean CLUSTer anaLySiS in riCe (Oryza SaTiVa L. SUB SPeCieS TrOPiCaL JaPOniCa) UnDer SaLineaLKaLine SOiLS OF inDia V.K. Yadav, K. Sidarath, S. Soni, L.P. Tiwari, T. Ram, V. Bhadana, M.C. Yadav (India)

64

P0199

grOWTH PrOMOTing-eFFeCTS OF L-?- PHenyLLaCTiC aCiD (LPa) in riCe (Oryza SaTiVe L.) SeeDLingS H. Watanabe, Y. Adachi (Japan)

P0200

grOWTH regULaTiOn OF riCe (Oryza SaTiVa L.) SeeDLing By giBBereLLiC aCiD anD eTHePHOn H. Watanabe, Y. Adachi (Japan)

P0201

yieLD reSPOnSe OF riCe (Oryza SaTiVa L.) genOTyPeS TO rePrODUCTiVe STage DrOUgHT STreSS UnDer rainFeD LOWLanD eCOSySTeM in eaSTern inDia S. KUMAR, S. SINGH, R. ELANCHEZHIAN, B.P. BHATT, O.N. SINGH (India)

P0202

PyraMiDing OF SaLiniTy anD SUBMergenCe TOLeranCe inTO ir64 BaCKgrOUnD THrOUgH MarKer-aSSiSTeD SeLeCTiOn (MaS) M. De Ocampo, R.E. Zantua, J.A. Egdane, A.M. Ismail (Philippines)

P0203

DiVerSiTy in P DeFiCienCy reSPOnSe rOOT TranSCriPTOMe aMOng riCe genOTyPeS aDaPTeD TO aCiDiC SOiLS OF nOrTH eaSTern HiLL regiOn OF inDia W. Tyagi, M. Rai, J.S. Yumnam, A.K. Dohling, F. Dkhar (India)

65





POSTer SeSSiOn

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13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No iDenTiFiCaTiOn OF a nOVeL QTL FOr TOLeranCe OF P0204 anaerOBiC gerMinaTiOn DeriVeD FrOM THe TOLeranT riCe LanDraCe, KaLOnCHi J.C.I. Ignacio, J.A. Tarun, M.J. Thomson, A.M. Ismail, E.M. Septiningsih (Philippines)

Poster No UnraVeLing THe regULaTOry rOLe OF TranSCriPTiOn P0210 FaCTOrS FOr enHanCing THe TOLeranCe LeVeL COnFerreD By SUB1a in riCe: an in SiLiCO aPPrOaCH A. Kumar, S. Heuer, A.S. Vidyarthi, D.M. Pandey (India

P0205

PerFOrManCe OF green SUPer riCe genOTyPeS in DrOUgHT PrOne nOrTHWeST BangLaDeSH T.H. ANSARI, H.U. Ahmed, J. Ali (Bangladesh)

P0206

COrreLaTeD reSPOnSe FOr DrOUgHT reSiSTanCe in riCe BeTWeen ManageD STreSS UnDer rain OUT SHeLTer anD HOT SPOT enVirOnMenT D. SHOBA, P. YOGA MEENAKSHI, P. Jeyaprakash, R. Pushpam, S. Robin (India)

P0207

eFFeCT OF eLeVaTeD CO2 On nUTrienT COnTenT, UPTaKe anD yieLD OF riCe CrOP - r. raJeSWari*, S. naTaraJan anD r. SiVaSaMy R. Ramanathan, S. Natarajan (India)

P0208

P0209

QTL anaLySiS FOr THe earLy-MOrning FLOWering CHaraCTer DeriVeD FrOM Oryza OFFiCinaLiS H. Hirabayashi, T. Kanbe, Y. Takemoto, Y. Takeuchi, T. Ogawa, I. Ando, T. Ishimaru (Japan) iDenTiFiCaTiOn OF MaJOr QTLS FOr grain yieLD UnDer DrOUgHT STreSS in JHUM riCe LanD raCeS OF nOrTH eaSTern inDia FOr USe in MarKer aSSiSTeD BreeDing S.P. DAS, N.P. Mandal, B.L. Meena, M.S. Anantha, A. Henry, A. Kumar, M. Datta, S.V. Ngachan (India)

66

P0211

inVeSTigaTiOn OF TriazOLe PeSTiCiDeS PrOPiCOnazOLe, TeBUCOnazOLe anD FenBUCOnazOLe FOr THeir aBiLiTy TO SUPreSS THe DeVeLOPMenT OF BaKanae DiSeaSe On riCe in in ViTrO COnDiTiOnS I. Karov, S. Mitrev, B. Kovacevik, E. Kostadinovska (Macedonia)

P0212

DeVeLOPMenT OF MaPPing POPULaTiOn FOr SaLiniTy TOLeranCe USing aT354 anD Bg352 riCe VarieTieS R. Dikkumburage, N.S. Kottearachchi, W.L.G. Samarasinghe (Sri Lanka)

P0213

zHOngzU14:a POTenTiaL green SUPer riCe genOTyPe FOr WaTer SaVing COnDiTiOn UnDer irrigaTeD eCOSySTeM H. Ahmed, S. Akhtar, H. Khatun, T. Ansari, J. Ali (Bangladesh)

P0214

PHySiOLOgiCaL reSPOnSeS TO HeaT STreSS in (Oryza SaTiVa L.):,SCreening OF niTrOgen, CHLOrOPHyLL COnTenT anD CeLL MeMBrane STaBiLiTy OF FLag LeaF M. Mombeini, R. Abdol Ali Gilani (Iran)

P0215

anaTOMiCaL CHangeS in rOOTS OF SeLeCTeD riCe genOTyPeS UnDer WaTer STreSS COnDiTiOn C. Cortaga, R. Sebidos (Philippines)

67





POSTer SeSSiOn

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Poster Area



Poster No TranSLaTing THe TranSCriPTOMe reSOUrCeS OF a P0216 MOnOCOT HaLOPHyTe SParTina aLTerniFLOra FOr iMPrOVing aBiOTiC STreSS TOLeranCe in riCe V.R. Mangu, R. Bedre, R. Joshi, E. Sanchez, B. Niranjan (USA)

Poster No aPPLiCaTiOn OF MOLeCULar BreeDing TO iMPrOVe P0222 SUBMergenCe TOLeranCe in VieTnaMeSe riCe CULTiVar TO COPe CLiMaTe CHange L. Le Hung, A. La Hoang, L. Nguyen Van, H. Le Huy, K. Tran Dang (Vietnam)

P0217

FUnCTiOnaL genOMiCS OF rOOTS TraiTS in eMS inDUCeD nagina-22 riCe POPULaTiOn S. Kambalimath, K. Chickkakariappa, R. Rangappa, S. Madavallam, T. Mohapatra, A.K. Singh, N. Sarala, U. Makarla, S. Robin, K. Singh, M.P. Rajanna, R.P. Sharma (India)

P0218

cDna-aFLP Transcriptomic Profiling of Upland and Lowland rice (Oryza sativa) Under Water Deficit H. HOSSEINI MONFARED, G. Xue, S. Ee, K. Loke, S. Kadkhodaei, P. Hedayati, L. Tan, I. Ismail, Z. Zainal (Malaysia)

P0219

CHaraCTerizaTiOn OF agrOnOMiC anD PHySiOLOgiCaL TraiTS FOr STagnanT FLOODing TOLeranCe in riCe J. Carandang, Z. Gonzaga, B. Collard, Y. Kato, E. Septiningsih, P. Sta Cruz, S.R. Das (Philippines)

P0220

MOLeCULar SCreening OF SaLT TOLeranT anD SenSiTiVe riCe genOTyPeS WiTH SaLTOL LinKeD SSr MarKerS G. Padmavathi, Y. Uma devi, S.A.H.U. Nihar, S.L. Krishna murthy, G. Gregorio, N. Sarla, T. Ram (India)

P0221

iDenTiFying nOVeL QTLS in ir42/Fr13a POPULaTiOn FOr SUBMergenCe TOLeranCe USing SnP MarKerS Z. Gonzaga, E. Suiton, D. Sanchez, J. Carandang, M. Thomson, D. Mackill, E. Septiningsih (Philippines)

68

P0223

BriDging THe yieLD gaP in PLanTS By gene PyraMiDing K.K. Sahoo, B. Gupta, A.K. Tripathi, A. Pareek, S.K. Sopory, S. SinglaPareek (India)

P0224

COMParaTiVe PrOTeOMiC anaLySiS in SaLT STreSSeD SHOOT TiSSUeS OF TWO COnTraSTing genOTyPeS OF riCe N. Lakra, S. Singla-Pareek, A. Pareek (India)

P0225

On-FarM ParTiCiPaTOry VarieTy SeLeCTiOn FOr SaLT TOLeranT riCe in MeKOng riVer DeLTa, VieTnaM N. Thi Lang, N. Thai Binh, B. Buu, R. Labios, R. Reinkee, A. Ismail, R. Wassmann (Vietnam)

P0226

inCreaSing riCe PrODUCTiViTy in SUBMergenCe- anD STagnanT FLOOD – PrOne areaS in MeKOng riVer DeLTa, VieTnaM N. Thi Lang, N. Van Hieu, P.T. Thu Ha, R. Labios, B. Chi Buu, R. Reinke, A. Ismail, R. Wassmann (Vietnam)

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BiOTiC STreSS reSiSTanCe

Poster No aDaPTaTiOn anD DiSSeMinaTiOn OF aCiD SULFaTe SOiL P0227 TOLeranT riCe in MeKOng riVer DeLTa, VieTnaM N. Thi Lang, B. Phuoc Tam, R. Labios, N. Van Hieu, P.T. Thu Ha, B. Chi Buu, R. Reinke, A. Ismail, R. Wassmann (Vietnam)

Poster No BLaST reSiSTanCe in aSian riCe (Oryza SaTiVa), aFriCan P0001 riCe (O. gLaBerriMa) anD WiLD riCe (O. BarTHii) OF WeST aFriCaT T. Teophile Odjo, D. Silue, Y. Koide, S. Yanagihaya, M. Sie, T. Kumashiro, Y. Fukuta(Japan)

P0228

insertional mutagenesis to isolate drought and salinity stress tolerant genes in rice S. Nugroho, U. Musofa, A. Zannati, D. Nurdiani, C.F. Pantouw, (Indonesia)

70

P0002

DiVerSiTy OF riCe gerMPLaSM FOr reSiSTanCe anD PaTHOgeniCiTy OF BLaST FUngUS in JaPan A. Tanaka-Kawasaki, Y. Fukuta (Japan)

P0003

DeVeLOPMenT OF MULTiLine VarieTieS FOr BLaST reSiSTanCe geneS WiTH inDiCa-TyPe geneTiC BaCKgrOUnDS M. Mary Jeanie Telebanco-Yanoria, Y. Yohei Koide, N. Nobuya Kobayashi, Y. Fukuta(Japan)

P0004

eFFeCT OF TeMPeraTUre On reSiSTanCe gene-MeDiaTeD reSPOnSe TO BaCTeriaL BLigHT OF riCe J.F. Balidion, I.R. Choi, R.P. Mauleon, P.H. Goodwin, K. Satoh, I.P.D. Navea, S. Kikuchi, K. Wydra, J.E. Leach, C.M. Vera Cruz

P0005

STUDy OF FerTiLiTy reSTOraTiOn OF neWLy DeVeLOPeD CMS LineS OF riCe (Oryza SaTiVa L.) anD aSSeSSMenT OF THeir reSiSTanCe TOBrOWn PLanTHOPPer (niLaParVaTa LUgenS STaL.) G. Prahalada, N. Shivakumar, B.P. Mallikarjuna, D.K. Sidde Gowda, P. Yadav (India)

P0006

iDenTiFiCaTiOn OF reSiSTanT genOTyPeS TO BaCTeriaL LeaF BLigHT OF riCe (Xanthomonas oryzae pv.oryzae) R. COUMARASAMY (India)

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BiOTiC STreSS reSiSTanCe (contd.)


Poster No MaPPing STeM rOT reSiSTanCe USing an aDVanCeD P0007 BaCKCrOSS POPULaTiOn C. Andaya, J. Oster, G. Yeltatzie, V. Andaya (USA)

Poster No P0013

P0008

FUnCTiOnaL MOLeCULar MarKerS FOr BaCTeriaL BLigHT reSiSTanCe gene Xa3 in riCe J. Lee, Y.J. Hur, J.H. Cho, H.S. Park, J.U. Jeung, J.Y. Lee, Y.B. Sohn, Y.C. Song, D.S. Park, M.H. Nam (Korea)

P0009

iMPrOVeMenT OF POPULar riCe VarieTy aDT 43 By MarKer aSSiSTeD BaCK CrOSS BreeDing By inTrOgreSSiOn OF BLaST reSiSTanCe geneS D. BALAKRISHNAN, S. ROBIN, R. RABINDRAN, S. SENTHIL, M. Raveendran, A.J. JOEL (India)

P0010

generaTiOn Mean anaLySiS OF yieLD anD BLaST reSiSTanCe reLaTeD TraiTS in riCe (Oryza SaTiVa L.) D. BALAKRISHNAN, A. BISWAS, S. ROBIN, R. RABINDRAN, A.J. JOEL (India)

P0011

eVaLUaTiOn OF nePaLeSe riCe gerMPLaSM FOr BLaST reSiSTanCe CHaraCTeriSTiCS R. Amgai, B. Sah, M. Bhatta (Nepal)

P0012

TraDiTiOnaL riCe LanD raCeS -POTenTiaL reSiSTanCe SOUrCeS FOr BreeDing VarieTieS TO BrOWn PLanTHOPPer, niLaParVaTa LUgenS (STaL.) D.K. Sidde Gowda, G.M. Dharshini, N. Shivakumar, P. Mahadevu (India)

72

Poster Area

MarKer-aSSiSTeD inTrOgreSSiOn OF BaCTeriaL BLigHT anD BLaST reSiSTanCe geneS inTO eLiTe inDian BaSMaTi VarieTieS G. Varaprasad, S. Srikanth, M. Pandey, M. Vijay Kumar, N. Shobha Rani, R.M. Sundaram, G.S. Laha, M. Sheshu Madhav, M. Srinivas Prasad, B.C. Viraktamath (India)

P0014

Large-SCaLe SCreening MeTHOD FOr eVaLUaTiOn OF BaKanae DiSeaSe in riCe D. Park, M. Kim, S. Lee, T. Kwon, W. Hwang, S. Park, D. Shin, Y. Youn, J. Lee, J. Cho, S. Han, U. Yeo, Y. Song, M. Nam (Korea)

P0015

MOLeCULar BaSiS OF riCe- gaLL MiDge inTeraCTiOnS D. Divya, Y. Tunginba Singh, S. Nair, J.S. Bentur (India)

P0016

iDenTiFiCaTiOn, MOLeCULar MaPPing anD MarKer-aSSiSTeD inTrOgreSSiOn OF nOVeL BaCTeriaL BLigHT reSiSTanCe geneS in riCe R. Sundaram, G.S. Laha, B.C. Viraktamath, M.S. Madhav, S.M. Balachandran, N.S. Rani, T. Ram, P. Natarajkumar, K. Sujatha, S.K. Hajira, A. Yugander, K. Pranathi, C.H. Balachiranjeevi (India) iDenTiFiCaTiOn anD CHaraCTerizaTiOn OF TaL eFFeCTOrS (TaLeS) reSPOnSiBLe FOr VirULenCe in Xa13-COMPaTiBLe inDian iSOLaTeS OF THe riCe BaCTeriaL BLigHT PaTHOgen, XanTHOMOnaS Oryzae PV. Oryzae (XOO) R. Sundaram, G.S. Laha, R.V. Sonti, A.J. Bogdanove (India)

P0017

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Poster No riCe PHenyLaLanine aMMOnia LyaSe 4 gene (OSPaL4) iS P0018 aSSOCiaTeD WiTH BrOaD SPeCTrUM DiSeaSe reSiSTanCe B.W. Tonnessen, P. Manosalva, J. Lang, J. Stephens, M. Baraoidan, A. Bordeos, J. Oard, S. Hulbert, H. Leung, J.E. Leach (USA)

Poster No Molecular tagging of brown plant hopper resistance locus in rice P0025 (Oryza sativa L.) B. Marathi, B. Sreevani, Kshirod K. Jena, Jhansi Laxmi V., Satyanarayana P.V., Suryanarayana Y. (Philippines)

P0019

Phenotyping of transgenic lines of indian rice cultivars against rice Tungro Disease P. Valarmathi, R. Velazhahan, S. Manonmani, S. Suresh, S. Robin, I. Dasgupta, R. Rabindran (India)

P0026

aVenUeS OF FUngiCiDaL inTerFerenCe DUring inFeCTiOn reLaTeD DeVeLOPMenT OF BiPOLariS Oryzae anD QTLS FOr riCe BrOWn SPOT A. KOTASTHANE, T. Agrawal, P.S. Nandanwar, U.S. Singh (India)

P0020

PHenOTyPiC SCreening OF SriLanKan riCe VarieTieS FOr BLaST reSiSTanCe W.A.D. Jayawardana, G.A.U. Jayasekera, R.L.C. Wijesundara, D.M.N. Dissanayake (Sri Lanka)

P0027

a SiMPLe inOCULaTiOn TeCHniQUe FOr Large SCaLe SCreening anD eVaLUaTiOn OF gerMPLaSM / BreeDing POPULaTiOn againST riCe SHeaTH BLigHT (rHizOCTOnia SOLani) DiSeaSe A. KOTASTHANE, T. Agrawal, U.S. Singh (India)

P0021

geOgraPHiC DiSTriBUTiOn OF aVirULenCe geneS in MagnaPOrTHe Oryzae in yUnnan PrOVinCe, CHina C. Li, J. Li, J. Yang, L. Liu, Y. Fukuta, Y. Zhu (China)

P0028

P0022

eXPreSSiOn PaTTern OF geneS enCODing SMaLL SeCreTeD PrOTeinS in niTrOgen STarVaTiOn MeDiUM anD DUring inTeraCTiOn OF BLaST FUngUS WiTH riCe J. Yang, C. LI, L. LIU, Y. ZHU, Y. DU, Z. SHI (China)

iDenTiFiCaTiOn OF nOVeL geneTiC reSOUrCeS FOr BrOWn PLanTHOPPer, riCe raggeD anD graSSy STUnT VirUS reSiSTanCe FrOM WiLD SPeCieS OF riCe R.B. Vinarao, J.M. Ramos, M.L. Ballesfin, S.S. Hechanova, M.M. Del Valle, R. Cabunagan, M. Balram, K.K. Jena (Philippines)

P0029

DiSSeCTing THe COrreLaTiOn BeTWeen LeaF BLaST anD PaniCLe BLaST By PHenOTyPing BC4F3 POPULaTiOn, MUTanTS anD MOnOgeniC LineS J. Yang, S. Zhang, X. Zhu, E. Ardales, L. Hei, B. Liu (China)

Development of pyramided lines with multiple resistance genes in indica rice S.S. Hechanova, J.M. Ramos, R.B. Vinarao, M.M. Del Valle, M. Balram, K.K. Jena (Philippines)

P0030

PrODUCTiOn OF near-iSOgeniC LineS FOr BPH reSiSTanCe geneS in inDiCa-TyPe riCe VarieTy, ir24 K.K. Jena, H. Verdeprado, D. Fujita, S.S. Hechanova, S. Sierra, D.S. Brar, P.S. Virk (Philippines)

P0023

P0024

aPPLy anD aSSeSSMenT OF a MarKer FOr BaCTeriaL BLigHT reSiSTanCe gene LOCUS Xa 27 in riCe P. Yu (China)

74

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Poster No Map-based cloning of Pi35, a gene for quantitative trait locus P0031 controlling blast resistance, identifies multiple functional polymorphisms in a disease resistance gene S. Fukuoka, S. Yamamoto, R. Mizobuchi, N. Kitazawa, U. Yamanouchi, N. Yasuda, Y. Fujita, T.T.T. Nguyen, K. Sugimoto, S. Koizumi, T. Matsumoto, M. Yano (Japan)

Poster No 14-3-3 PrOTeinS (gF14B anD gF14e) FUnCTiOn in PaniCLe P0037 BLaST reSiSTanCe in riCe Q. Liu, A.Q. Feng, J.L. Zhao, S.H. Zhang, J.Y. Yang, X.Y. Zhu, J.E. Leach, H. Leung, B. Liu (China) P0038

aSSOCiaTiOn MaPPing OF reSiSTanCe TO BaCTeriaL BLigHT (XanTHOMOnaS Oryzae PV. Oryzae) in DiVerSe riCe gerMPLaSM C.J. Dilla-Ermita, E.S. Tandayu, D. Lozada, L. Nora, V. Bartolome, C. Vera Cruz, E.Y. Ardales, M.G. Diaz, M.S. Mendioro, M.J. Thomson (Philippines)

P0039

anaLySiS OF aLLeLiC VariaTiOnS in riCe BLaST reSiSTanCe gene Pi54 anD PaTHOgen aVirULenCe gene aVrPi54 HeLPS in UnDerSTanDing THeir CO-eVOLUTiOn T.R. Sharma, S. Thakur, S. Ray, P.K. Singh, A.K. Singh, M. Variar, U.D. Singh, R. Rathour, S.K. Prashanthi, N.K. Singh (India)

P0032

HOST PLanT reSiSTanCe againST riCe WHiTeBaCKeD PLanTHOPPer, SOgaTeLLa FUrCiFera (HOrVaTH) S. Seetharaman (India)

P0033

reSiSTanCe OF PHiLiPPine reLeaSeD riCe VarieTieS TO BaCTeriaL BLigHT M. Burgos, I. Oña, C. Vera Cruz (Philippines)

P0034

CySTeine PrOTeaSe gene FrOM BraSSiCa raPa iMPrOVeS reSiSTanCe TO BaCTeriaL LeaF BLigHT in TranSgeniC riCe M.C. Nino, S.E. Abdula, H.J. Lee, D.A. Yu, S.U. Byeon, F.C. Nogoy, K.K. Kang, I.S. Nou, Y. Cho (Korea)

P0040

geneTiC iMPrOVeMenT OF MainTainer LineS FOr MULTiPLe BaCTeriaL BLigHT reSiSTanCe geneS anD gOOD grain QUaLiTy FOr PHiLiPPine HyBriDS I.G. Pacada, F.P. Waing, J.J. Unay, C.P. Quinones, L.P. Coloma, D.A. Tabanao (Philippines)

reCeSSiVe anD DOMinanT reSiSTanCe TO TWO TUngrO VirUSeS CO-eXiSTing in riCe CULTiVar UTri MeraH I.R. Choi, G.A. Atienza-Grande, J.H. Shim, R.J.A. Macalalad, J.H. Lee, J.R. Encabo, R.C. Cabunagan, K. Satoh, I.H. Slamet-Loedin, Y. Kishima, S. Kikuchi, H. Leung (Philippines)

P0041

MTU iTJ 206-7-4-1, a Strong Donor for Plant Hopper resistance in rice. P. Venkata satyanarayana, N. Chamundeswari, B. Balakrishna, B. Ravikumar, K. Vasantha bhanu, M. Girijarani, P. Ramanarao, M. Barath laxmi (India)

P0035

P0036

iDenTiFiCaTiOn OF nOVeL reSiSTanCe SOUrCeS FOr BaCTeriaL DiSeaSeS in riCe USing a MULTi-ParenT reCOMBinanT POPULaTiOn A.M. Bossa-Castro, C. Raghavan, E.E. Delorean, C.M. Vera Cruz, H. Leung, G. Mosquera, V. Verdier, J. Leach (USA)

76

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Poster No identification of molecular determinants of virulence during P0042 interaction of rice to two strains of Xanthomonas oryzae pv. oryzae G. Grande, I. Quibod, S. Dossa, F. Borja, P. Capistrano, R. Mauleon, C. Vera-Cruz, R. Oliva (Philippines)

Poster No P0048

P0043

genetic dissection of resistance to green leafhopper and rice tungro spherical virus in cultivar arC11554 A. Dela Cruz, J. Lee, R. Cabunagan, D. Fujita, G. Atienza-Grande, E. Mendoza, D. Tabanao, H. Yasui, I. Choi (Philippines)

P0044

PCr-based Dna markers for rapid discrimination of single nucleotide polymorphisms in the translation initiation factor 4gamma gene conferring resistance to rice tungro spherical virus G. Grande, J. Lee, S. Kim, R. Cabunagan, A. Dela Cruz, J. Shim, D. Fujita, I. Choi (Philippines)

P0045

MOLeCULar MaPPing OF a MaJOr gene COnFerring reSiSTanCe TO green riCe LeaFHOPPer, nePHOTeTTiX CinCTiCePS UHLer, DeriVeD FrOM an inDiCa riCe CULTiVar aSD7 T. Mai Van, A. Yoshimura, H. Yasui (Japan)

P0046

iSOLaTiOn anD CHaraCTerizaTiOn OF riCe LeSiOn MiMiC anD SeneSCenCe MUTanT (LMS) WiTH enHanCeD reSiSTanCe TO riCe BLaST (MagnaPOrTHe Oryzae) J. Undan, M. Tamiru, A. Abe, H. Takagi, J.Q. Undan, H. Yaegashi (Philippines)

P0047

DeVeLOPMenT OF near iSOgeniC LineS FOr BLaST reSiSTanCe in BaSMaTi riCe A. Khanna, A.B. Shikari, S. Gopala Krishnan, U.D. Singh, T.R. Sharma, R. Rathour, M. Variar, S.K. Prashanti, A.K. Singh (India)

78

Poster Area

MarKer aSSiSTeD PyraMiDing OF MaJOr BLaST RESISTANCE GENES IN BASMATI RICE VARIETY ‘PUSA BASMATI 1’ A.B. Shikari, A. Khanna, S. Gopala Krishnan, U.D. Singh, T.R. Sharma, R. Rathour, M. Variar, S.K. Prashanti, K.V. Prabhu, A.K. Singh (India)

P0049

PyraMiDing OF BLaST reSiSTanCe geneS in THe BaSMaTi riCe VarieTy PUSa BaSMaTi 1121 THrOUgH MarKer aSSiSTeD BaCKCrOSS BreeDing R.K. Ellur, S. Pathania, S. Gopalakrishnan, P.K. Bhowmick, M. Nagarajan, K.K. Vinod, U.D. Singh, R. Rathour, K.V. Prabhu, A. Singh (India)

P0050

MOLeCULar CHaraCTerizaTiOn OF riCe LanDraCeS OF KarnaTaKa, inDia FOr BLaST reSiSTanCe P. S.K, K. Ingole, K. PU (India)

P0051

MarKer aSSiSTeD reCUrrenT SeLeCTiOn(MarS) FOr iMPrOVing BiOTiC STreSS reSiSTanCe OF geneTiC MaLe STeriLe reSTOrer POPULaTiOn P. Revathi, S. Janardhan, S. Arun Kumar, P. Senguttuvel, K.B. Kempa Raju, R.M. Sundaram, A.S. Hari Prasad, B.C. Viraktamath (India)

P0052

PyraMiDing OF MaJOr Pi geneS in riCe FOr DUraBLe reSiSTanCe againST DiVerSe BLaST raCeS in nOrTH eaSTern HiLL regiOn OF inDia M. Rai, G.F. War, M.R.J. Najiar, B. Kumari, W. Tyagi (India)

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genOMiC DiVerSiTy

Poster No a FirST generaTiOn MiCrOSaTeLLiTe- anD SnP-BaSeD P0053 LinKage MaP OF BrOWn PLanTHOPPer J. Jairin, T. Kobayashi, M. Matsumura, H. Yasui (Thailand)

Poster No HarneSSing FUnCTiOnaL genOMiCS & neW aLLeLiC P0056 DiVerSiTy in PrODUCT DeVeLOPMenT V. Knauf, D. Facciotti, C. McGuire, D. Loeffler (USA)

P0054

Development of biotic stress resistant MTU 1010 using marker assisted breeding D. Ch.V., M.D. Jamaloddin, G. Swathi, S. Vanisree (India)

P0057

geneTiC DiVerSiTy OF SOMe BangLaDeSHi aUS riCe genOTyPeS M.E. Hoque, M.M. Islam, S. Sultana, J. Ferdous, M.S. Ali (Bangladesh)

P0055

The development of transgenic rice lines with potential resistant to yellow stem borer and their impact on biodiversity of non-target organisms in confined field trial A. Estiati, P. Lisdiyanti, S. Nugroho, Y. Rahayuningsih, N. Usyati, T. Chrismada, G.P. Yoga (Indonesia)

P0058

geneTiC DiVergenCe anaLySiS USing yieLD anD yieLD reLaTeD TraiTS in riCe (Oryza SaTiVa L.) N.P.S. De Silva, B.P.S. Malik, K.R. Battan (Sri Lanka)

P0059

aLLeLiC DiVerSiTy BaSeD On MiCrOSaTeLLiTe MarKerS LinKeD TO SHeaTH BLigHT reSiSTanCe in riCe (Oryza SaTiVa L.) M.K. Hossain, M.A.R. Bhuiyan, R. Wickneswari (Malaysia)

P0060

aSSeSSMenT OF HOW geneTiCaLLy reLaTeD 10 riCe CULTiVarS CULTiVaTeD in BrUnei USing MiCrOSaTeLLiTe MarKerS N. Ishak, Z. Sulaiman, K. Tennakoon (Brunei)

P0061

genOMe WiDe aSSOCiaTiOn STUDy (gWaS) FOr agrOnOMiC anD yieLD-COMPOnenT TraiTS USing a SeT OF eLiTe irrigaTeD BreeDing LineS H. Begum, V. Lopena, V. Bartolome, P. Virk, G. Gregorio, J. Spindel, S. McCouch, B. Collard (Philippines)

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genOMiC DiVerSiTy (contd.)


Poster No P0062

Poster No DEVELOPMENT OF “KEY MARKER” CONCEPT FOR PREDICTION P0068 anD VaLiDaTiOn OF HeTerOSiS USing MiCrOSaTeLLiTe MarKerS in ParenTaL LineS OF riCe (Oryza SaTiVa L). S. SONI, R.M. SUNDARAM, V.K. Yadav, V.P. Bhadana (India)

SHOTgUn PrOTeOMiCS aPPrOaCH TO MOniTOr PrOTeOMe eXPreSSiOn PaTTernS OF riCe anTHerS DUring DeVeLOPMenT H. Kim, J. Cho, J. Lee, J. Lee (Korea)

P0063

SearCHing FOr TOLeranCe TO P DeFiCienCy in aFriCan riCe (O. gLaBerriMa). a SUrVey FOr THe PreSenCe OF P STarVaTiOn TOLeranCe gene (PSTOL1). J. Pariasca-Tanaka, K.N. Drame, J.H. Chin, S. Heuer, M. Wissuwa (Japan)

P0064

raiSing inTrOgreSSiOn LineS FrOM aa genOMe SPeCieS OF genUS Oryza FOr gene DiSCOVery anD geneTiC iMPrOVeMenT J. Zhou, P. Xu, J. Li, Y. Zhang, X. Deng, F. Hu, W. Deng, Z. Wu, J. Zhao, Y. Yu, Y. Yang, D. Tao (China)

P0065

P0066

P0067

QTL MaPPing OF PaniCLe TraiTS USing inTrOgreSSiOn LineS FrOM aa-genOMe SPeCieS P. Xu, Y. Yu, J. Zhou, J. Li, Y. Zhang, D. Tao (China) aSSeSSMenT OF geneTiC VariaBiLiTy OF 17 OLD anD neW reLeaSeD LOWLanD riCe VarieTieS in inDOneSia USing 384 SnP MarKerS U. Susanto, N.A. Rohmah, P. Lestari, I.M.J. Mejaya (Indonesia) SOrgHUM MUTageneSiS: UnVeiLing geneS OF C4 PHOTOSynTHeSiS R. Garcia, G. Rizal, K. Acebron, N.A. Larazo, M. Alcasid, N. Elayda, J. Salonga, A. Mabilangan, M.J. Dionora, W.P. Quick (Philippines)

82

P0069

genOTyPing OF WOrLD Mini COre COLLeCTiOnS, THeir UniQUe iDenTiFiCaTiOn anD geneTiC SiMiLariTy USing MiCrOSaTeLLiTe MarKerS in riCe (Oryza SaTiVa L.). S. SONI, R.M. SUNDARAM, V.K. Yadav, V.P. Bhadana (India)

P0070

aLLeLe LaDDer FOr PHiLiPPine riCe VarieTaL iDenTiFiCaTiOn V. Dalusong, L. Santos, L. Perez (Philippines)

P0071

CUrrenT DiVerSiTy OF ParenTaL geneTiC STOCKS USeD in riCe BreeDing in THe PHiLiPPineS L.M. Perez, M.I.C. Calayugan, T.E. Mananghaya, J.A. Orcino, T.F. Padolina (Philippines)

P0072

eVaLUaTiOn OF geneTiC DiVerSiTy aMOng riCe VarieTieS in CaMBODia C. Orn, R. Ishikawa, S. Sakhan, M. Ouk, T. Ishii (Japan)

P0073

BUiLDing THe 12K COre FOr THe riCe geneTiC DiVerSiTy PLaTFOrM M.E.B. Naredo, S.M.Q. Mercado, F.C. De Guzman, R.A. Reaño, G.S. Capilit, N.R. Sackville Hamilton, K.L. McNally (Philippines)

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Poster Area

13:30 - 15:00



Poster No aSSeSSMenT OF geneTiC VariaTiOn anD DeVeLOPMenT OF P0074 CULTiVar MiXTUreS FOr rainFeD anD UPLanD riCe eCOSySTeM L.M. Perez, R.M.F. Cabanting, V.G. Dalusong, I.G. Pacada (Philippines)

Poster No P0080

Poster Area

MinOKaWa: a PiPeLine FOr COMParaTiVe anaLySeS OF DraFT genOMeS V.J. Ulat, F.N. Borja, J. Detras, V. Juanillas, R.R. Fuentes, R.J.R. Santos, R. Mauleon, P. Tanger (Philippines)

P0075

riCe SHeaTH BLigHT: iDenTiFiCaTiOn OF QUanTiTaTiVe reSiSTanCe THrOUgH aSSOCiaTiOn geneTiCS E.G. Oreiro, G. BELIGAN, J. AGUIRRE, S. SRINIVASACHARY, S. SAVARY, L. WILLOCQUET, R. OLIVA (Philippines)

P0081

FinDing C4 TO C3 reVerTanTS in a MUTageniSeD SeTaria ViriDiS POPULaTiOn By STUDying anaTOMiCaL LeaF aBerraTiOnS N. Larazo, G. Rizal, K. Acebron, R. Garcia, W.P. Quick (Philippines)

P0076

CaPTUring VariaTiOn in riCe PHOTOSynTHeSiS I. CANICOSA, G. Dimayuga, J. Chatterjee, M. Dionora, W.P. Quick (Philippines)

P0082

P0077

JirCaS reSearCH aCTiViTieS reLaTeD TO riCe geneTiCS in griSP T. Ishimaru, T. Ishimaru, Y. Fukuta, N. Kobayashi, N. Kobayashi, M. Obara, M. Wissuwa, S. Yanagihara (Philippines)

LOSS-OF-FUnCTiOn OF an UBiQUiTin-reLaTeD MODiFier rUrM1 PrOMOTeS THe MOBiLizaTiOn OF THe aCTiVe MiTe MPing T. Tsukiyama, S. Teramoto, K. Yasuda, A. Horibata, N. Mori, Y. Okumoto, M. Teraishi, H. Saito, A. Onishi, K. Tamura, T. Tanisaka (Japan)

P0083

geneTiC OVerLaPS BeTWeen DrOUgHT TOLeranCe anD SHeaTH BLigHT reSiSTanCe in riCe (Oryza SaTiVa L.) T. Zheng, W. Wang, L. Hoang, F. Li, L. Huang, L. Zhu, Y. Sun, J. Xu, Z. Li (China)

P0084

aSSOCiaTiOn MaPPing OF yieLD anD DiFFerenT agrOnOMiC TraiTS in STrUCTUreD POPULaTiOn OF riCe genOTyPeS M. Jahani, G. Nematzadeh, G. Mohammadi-Nejad (Iran)

P0078

P0079

Dna anaLySiS reVeaLS HigH aMOUnT OF MOLeCULar VariaTiOn anD POPULaTiOn DiFFerenTiaTiOn in arOMaTiC riCe LanDraCeS (Oryza SaTiVa L. SSP inDiCa) FrOM UTTar PraDeSH, inDia M. Yadav, M. Mittal, S. Tiwari, V. Yadav, K. Srinivasan (India) TaLUSi: an HDF5 TOOL FOr eFFiCienT STOring anD QUerying OF Large SnP DaTaSeTS R.R. Fuentes, K. McNally, R. Mauleon, V.M. Juanillas, N. Alexandrov (Philippines)

84

85





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area

grain QUaLiTy anD nUTriTiOn

grain QUaLiTy anD nUTriTiOn (contd.)

Poster No reSearCH FOr BreeDing OF eXPOrT riCe VarieTieS FOr P0085 VieTnaM L. NGUYEN THI, L.U.Y. Trinh thi Luy, X.A. Tran thi Thanh, P. Tam bui, C.H.I. Buu bui (Vietnam)

Poster No riCe BiOTeCHnOLOgy: aPPLiCaTiOn OF MiCrOSaTeLLiTe P0091 MarKerS FOr SCreening anD iDenTiFiCaTiOn OF irOn riCH riCe genOTyPeS L. HASSAN, G.S. Jahan, S.N. Islam, M.S. Alam, R. Gain (Bangladesh)

P0086

aSSOCiaTiOn BeTWeen aLLeLeS OF THe FragranCe gene anD arOMaTiC COMPOUnDS in aSian riCe (Oryza SaTiVa L.) J.C.T. Concepcion, C.C. Llorente, V.D. Daygon, N. Sreenivasulu, M.A. Fitzgerald (Philippines)

P0092

Fine MaPPing OF THe LOW-aMyLOSe CHaraCTeriSTiCS OF riCe CULTiVarS BaegJinJU M.H. Nam, J. Lee, Y.J. Hur, S.Y. Kim, D.S. Park, J.H. Cho, J.Y. Lee, Y.B. Sohn, Y.C. Song (Korea)

P0087

eFFeCT OF DiSSOLVeD CaLCiUM CarBOnaTe in SOaKing WaTer FOr ParBOiLeD riCe QUaLiTy C. Gunathilake (Sri Lanka)

P0093

TarOM geneTiCaLLy MODiFieD riCe iS nOn aLLergeniC A. Najaf (Iran)

P0088

anTiOXiDanT anD anTiMiCrOBiaL PrOPerTieS OF CHaK-HaO - a DarK PUrPLe gLUTinOUS arOMaTiC riCe OF ManiPUr, inD ia. M. Kangabam (India)

P0094

TOCOPHerOL anD TOCOTrienOL COnTenT in KOrean JaPOniCa anD TOngiL TyPe riCe J.S. Lee, M.R. Yoon, J. Kwak, J.H. Lee, S.W. Yoon, A. Chun, J. Lee, I.H. Kim (Korea)

P0089

COnFineD FieLD TeST OF PrO-ViTaMin a enriCHeD 'gOLDen riCe' eVenT gr2-r inTrOgreSSeD LineS OF ir64 anD PSB rC82 A.A. Alfonso, J.J. Somera, E.O. Espejo, R.T. Miranda, E.S. Avellanoza, D.A. Tabanao, E.S. Nazareno, N.C. Ramos, M.V. Romero (Philippines)

P0095

inVeSTigaTiOn OF WaTer aBSOrPTiOn, COOKing anD TeXTUre PrOPerTieS OF BrOWn riCe FOr LOW-energy inPUT USing Ten KOrean CULTiVarS OF JaPOniCa TyPe A. Chun, M.R. Yoon, J.H. Lee, S.K. Oh, J. Kwak, J.S. Lee, B.K. Kim (Korea)

P0090

MarKer aSSiSTeD inTrOgreSSiOn OF gOLDen riCe TraiT inTO a Mega riCe VarieTy OF BangLaDeSH P. Biswas, M.A.H. M. A. Hossain, M.I. M. Inabangan, J.M. J. Macabenta, V.A. V. Aldemita, V.L. V. Lacorte, L.T. L. Torrizo, M.S. M. Samia, J.T. J. Tan, R.B. R. Boncodin, T.S.B. T. S. Bharaj, G.B. G. Barry, P.V. P. Virk (Bangladesh)

P0096

iDenTiFiCaTiOn anD MOLeCULar MaPPing OF MaJOr QTLS COnTrOLLing PHySiCO-CHeMiCaL QUaLiTy TraiTS in riCe S. MAGANTI, S. Ramadevi, B. Venkaiah, S.K. Hajira, K. Pranathi, S. Srikanth, V. Ravindra Babu, R.M. Sundaram, N. Shobha Rani, B.C. Viraktamath (India)

86

87





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No iMPrOVing OrganiC PHOSPHaTe aCQUiSiTiOn in P0097 TranSgeniC riCe M. Nam, S. Park, D. Shin, D. Park, D. Kim, W. Hwang (Korea)

Poster No P0104

P0098

evaluation of semi dwarf Basmathi rice (Oryza sativa L.) germplasm for quality rice improvement G. Abeysundara Hewawasamge (Sri Lanka)

P0099

grain QUaLiTy aTTriBUTeS OF arOMaTiC riCeS STOreD HerMeTiCaLLy R. Hafeel, D.M.N. Dissanayake, B.D.R. Prasantha, E.M.R.D. Edirisinghe (Sri Lanka)

P0100

CHaraCTerizaTiOn OF aPParenT aMyLOSe anD PaSTing PrOPerTieS in MyanMar riCe CULTiVarS N. Nyo Nyo Mar, T. Umemoto, M. Maziah, A. Siti (Myanmar (Burma)

P0101

P0102

P0103

eFFeCT OF DiFFerenT PrOCeSSing anD COOKing MeTHODS On THe SeLeCTeD MineraL COnTenT OF riCe (Oryza SaTiVa L.) S. Dipti, W. Hurtada, M. Fitzgerald (Bangladesh) QTL MaPPing FOr irOn anD zinC COnTenT in DOngXiang WiLD riCe (Oryza rUFiPOgOn griFF.) D.R. Huang, B.L. Hu, Y.Y. Fan, J.Z. Ying, Y.J. Zhu (China) geneTiC enHanCeMenT OF riCe FOr MiCrOnUTrienTS THrOUgH BiOFOrTiFiCaTiOn R. Vemuri, T. Longvah, C.N. Neeraja, K. Surekha, S.R. Durbha, N.S. Rani, B.C. Viraktamath (India)

88

WHiTe riCe, iTS PHySiCaL aPPearanCe anD QUanTiTaTiVe LOSS Y. Aung (Thailand)

P0105

aSSOCiaTiOn anaLySiS FOr SOMe nUTrienTS COnTenTS USing riCe COre SeT. S.W. Kwon, X.Q. Wang, W.H. Ra, Y.H. Cho, Y.C. Kim, Y.S. Lee, Y.J. Park (Korea)

P0106

geneTiC VariaTiOn FOr grain PrOTein COnTenT, grain QUaLiTy anD nUTrienT QUaLiTy ParaMeTerS USing reCOMBinanT inBreD POPULaTiOn in riCe (Oryza SaTiVa L.) S. Narayana Naik, S. Hittalmani (India)

P0107

anTiOXiDanT CaPaCiTy anD anTiOXiDanT COMPOnenTS OF BLaCK riCe DUring iTS grain DeVeLOPMenT J. Kwak, J.H. Lee, M.R. Yoon, A. Chun, S.K. Oh, J. Chang, J.S. Lee, B.K. Kim (Korea)

P0108

anTiOXiDanT aCTiViTieS OF riCe Bran eXTraCTS anD SiKHye inCLUDing riCe Bran eXTraCTS J.H. Lee, D.J. Kim, M.R. Yoon, A. Chun, S.K. Oh, J. Kwak, J.S. Lee, B.K. Kim (Korea)

P0109

SeeD CHaraTeriSTiCS OF SOMe FUnCTiOnaL PigMenTeD riCe S.J. Han, T.H. Ham, S.N. Ryu (Korea)

89





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No aSSOCiaTiOn MaPPing FOr grain Size TraiTS in riCe P0110 D. Deborah, P. Santosh, V. Roja, G. Anuradha, E.A. Siddiq, L.R. VEMIREDDY (India) DOWn-regULaTiOn OF SSS1 anD gBSS1 gene May CHange P0111 THe aMyLOSe anD aMyLOPeCTin COnTenTS in TranSgeniC riCe M.G. Jee, H.J. Lee, M.M. Sun, Y.J. Jung, S.E. Abdula, D.W. Jang, D.A. Yu, K.K. Kang, I.S. Nou, Y.G. Cho (Korea)

Poster No MaPPing OF QUanTiTaTiVe TraiT LOCi FOr zinC COnTenT in P0116 riCe O.Y. Jeong, Y.A. Jeon, H.S. Lee, W.Y. Song, K.S. Lee, J.H. Lee, M.K. Kim, Y.J. Won, S.N. Ahn* (Korea)

P0112

eFFeCT OF Pre-SOaKing COnDiTiOnS anD VarieTy On THe PHySiCaL anD CHeMiCaL QUaLiTy OF PaBOiLeD riCe O. Adeniran, O. KAJIHAUSA, H.A. Alamele (Nigeria)

P0113

aSSOCiaTing PaSTing PrOPerTieS OF POPULar riCe VarieTieS OF inDia WiTH THe enD USe QUaLiTy. S. Manonmani, S. Robin (India)

P0114

eFFeCT OF BraDyrHizOBiUM (OrS278) inOCULaTiOn On THe yieLD COMPOnenTS OF Sri LanKan iMPrOVeD riCe VarieTy aT 308 S. Geekiyanage, H. Kumara, N. Ahlgren, E.P. Greenberg, G. Senanayake (Sri Lanka)

P0115

STUDieS On HeTerOSiS OF QUaLiTy TraiTS in riCe (Oryza SaTiVa L.) C. NARNE, P. Satyanarayana, Y. Suryanarayana, K. Rajareddy, P. Jayaramireddy, V. Srinivasarao (India)

90

P0117

Stability analysis of grain iron and zinc ciontent in rice genotypes using gge biplot method G. Nirmala Devi, V. Ravindra Babu, W. Kavita, G. Padma Vathi (India)

P0118

BreeDing OF HigH zinC riCe in BangLaDeSH M. Hossain, P.S. Biswas, S.A. Islam (Bangladesh)

P0119

STaBiLiTy anaLySiS OF PrOTein COnTenT in TraDiTiOnaL LanD raCeS OF riCe N.G. HANAMARATTI, T.N. SATHISHA, P.M. SALIMATH, S. HITTALMANI, P. SURENDRA (India) TraDiTiOnaL LanDraCeS OF riCe in KarnaTaKa: VaLUaBLe SOUrCeS OF irOn anD zinC N.G. HANAMARATTI, T.N. SATHISHA, P.M. SALIMATH, V. RAVINDRA BABU, B.C. VIRAKTMATH (India)

P0120

P0121

anaLySiS OF grain QUaLiTy TraiTS in inDiCa riCe HyBriDS M. Umadevi, P. Veerabadhiran, S. Manonmani (India)

P0122

THe rOLe OF THe Oryza SaTiVa THiOreDOXin H PrOTein PrOMOTer (PrOSTrXH) in TranSLOCaTing irOn WiTHin riCe grain C. Adeva, G.M. Bueno, F.M. Arines, K.R. Trijatmiko, M. Manzanilla, M.G. Borja, L. Torrizo, N. Tsakirpaloglou, P. Chadha-Mohanty, I.H. Slamet-Loedin (Philippines)

91





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00


BreeDing aPPLiCaTiOnS

Poster No gene PyraMiDing USing niCOTianaMine SynTHaSe anD P0123 FerriTin: an aPPrOaCH TO inCreaSe THe irOn COnCenTraTiOn in THe riCe enDOSPerM J. Balindong, K. Trijatmiko, L. Torrizo, P. Malabanan, M. Manzanilla, G. Borja, M. Mendioro, M. Dionisio-Sese, G. Barry, I. Slamet-Loedin (Philippines)

Poster No P0128

P0124

P0125

P0126

grain yieLD anD QUaLiTy ParaMeTerS OF CULTiVarS aS inFLUenCeD By SySTeM OF riCe inTenSiFiCaTiOn (Sri) MeTHOD VS COnVenTiOnaL FLOODeD MeTHOD in riCe M. Rapolu, R. Vemula, V. Lella, S. Kuchi, P. Chintahala, S. Nethi, R. Puskur, S. Madamsetty, R. Sunderam, M. Pichiayya pillai, C. Basavanneyya (India) DeVeLOPMenT OF HigH grain zinC riCe VarieTieS TO aLLeViaTe zinC MaLnUTriTiOn M. Asilo, A. Amparado, C. Manito, F. Tesoro, V. Lopena, J. Rey, G. Barry, P. Virk, B.P.M. Swamy (Philippines)

MarKer-aSSiSTeD SeLeCTiOn in riCe BreeDing FOr HeaT TOLeranCe in VieTnaM B. BUI CHI, L.A.N.G. NGUYEN THI (Vietnam)

P0129

PrOgreSS OF riCe BreeDing reSearCH in anDHra PraDeSH, inDia P.R.O.F. Chandragiri cheralu (India)

P0130

STUDying THe inHeriTanCe OF WX/WX gene USing MarKer in COnTrOLLing gLUTinOUS OF BC3F2 riCe PLanT. T. Vo van, V. Sangtong, A. Joomwong (Vietnam)

P0131

BreeDing FOr riCe SUBMergenCe TOLeranCe anD STagnanT FLOOD TOLeranCe in VieTnaM L. NGUYEN THI, N. Hieu van, T. Thi Nhien, C.H.A.U. Thanh Nha, B. ChiBuu (Vietnam)

P0132

geneTiC anaLySiS FOr HeaT TOLeranCe anD earLy MOrning FLOWering aT FLOWering STage in riCe (Oryza SaTiVa L.) N.N.A. Baliuag, E.D. Redoña, J.E. Hernandez, P.C. Sta Cruz, C. Ye (Philippines)

P0133

raPiD generaTiOn aDVanCe: HOW raPiD in DeVeLOPing aDVanCeD BreeDing LineS FOr HeaT TOLeranCe? N. Manigbas, L.B. Madrid, L.A.F. Lambio (Philippines)

engineering BiO-FOrTiFieD riCe WiTH HigH LySine COnTenT M. Mishra, A. Pareek, S. Singla-Pareek (India)

92

Poster Area

93





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area

BreeDing aPPLiCaTiOnS (contd.)


Poster No aDaPTaBiLiTy OF KOrean CULTiVarS in LOCaTiOn-SPeCiFiC P0134 areaS in THe PHiLiPPineS N. Manigbas, L.B. Madrid, A.M. Corales, E.H. Vargas, L.M. Pamugas, J.T. Lee (Philippines)

Poster No inTra-SPeCiFiC BreeDing OF aFriCan riCe (Oryza P0140 gLaBerriMa STeUD.) TO TaiLOr VarieTieS FOr reSOUrCePOOr FarMerS in WeST anD CenTraL aFriCa M. SIE, Y. AGNOUN, K. FUTAKUCHI, N. ZENNA, R. VODOUHE, F. CISSE (Nigeria)

P0135

STUDy On THe FLOWering TiMe CHaraCTerS OF an earLy FLOWering TiMe riCe Line eST-1 anD THe ParenTS OF inDiCa HyBriD riCe (Oryza SaTiVa L.) H. Kang, G. REN (China)

P0136

iDenTiFiCaTiOn OF QUanTiTaTiVe TraiT LOCi (QTL) FOr reSiSTanCe TO WHiTe-BaCKeD PLanTHOPPer (SOgaTeLLa FUrCiFera) WiTH BaCKgrOUnD OF TOngiL riCe VarieTy MiLyang 46: (CHeOngCHeOngByeO) G. Yi, J.K. Sohn, T.H. Kim, M. Norvie (Korea)

P0137

DeVeLOPMenT OF SUgary riCe FOr FUnCTiOnaL PUrPOSeS Y.C. Song, J.H. Cho, U.D. Suh, J.H. Lee (Korea)

P0138

MrQ 76 : neW MaLaySian FragranT riCe VarieTy WiTH JaSMine TyPe CHaraCTeriSTiCS A. Ramli, M. Hashim, O. Omar, E. Sulaiman, S. Misman, S. Hashim, M. Mohd Yusof (Malaysia)

P0139

CLOUD-BaSeD reSearCH DaTa rePOSiTOry: iMPrOVeD OnLine aCCeSS TO yearS OF VaLUaBLe riCe BreeDing inFOrMaTiOn anD KnOWLeDge E. Mercado, D. Arreza, M. Bernardo (Philippines)

94

P0141

a SiMPLe MeTHOD TO OBTain PCr-reaDy eXTraCTS FOr THe DeTeCTiOn OF XanTHOMOnaS Oryzae PaTHOVarS Oryzae anD OryziCOLa COnTaMinaTiOn in riCe SeeDS M.H.R. Nguyen, J.M. Lang, M.R.G. Burgos, B. Cottyn, D. Mishra, Y. Raj, V. Verdier, J.E. Leach, C.M. Vera Cruz (Philippines)

P0142

HarneSSing riCe FUnCTiOnaL genOMiCS inTO VarieTy DeVeLOPMenT THrOUgH TiLLing V. Knauf, C. Moehs, J. Lewis (USA)

P0143

THe USe OF Dna MarKerS in BreeDing FOr iMPrOVeD CaLiFOrnia riCe VarieTieS C. Andaya, F. Jodari, S. Samonte, J. Oster, K. McKenzie, G. Yeltatzie, V. Andaya (USA)

P0144

aPPLiCaTiOn OF Dna MarKerS FOr THe DeTeCTiOn OF aMyLOSe COnTenT in Sri LanKan riCe (Oryza SaTiVa L.) VarieTieS N. Kottearachchi, R.K. PEIRIS, S. REBEIRA (Sri Lanka)

P0145

iDenTiFiCaTiOn OF QTLS FOr DayS TO HeaDing in an UPLanD neW riCe FOr aFriCa (neriCa) VarieTy Y. Koide, A. Tanaka-Kawasaki, T. Odjo, M. Obara, S. Yanagihara, Y. Fukuta (Japan)

95





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No P0146

Poster No FarMer ParTiCiPaTOry riCe BreeDing PrOgraMMe in Sri P0151 LanKa P.V. Hemachandra, W.G. Chaminda Lal Kumara, A.P. Bentota (Sri Lanka)

P0147

P0148

DeVeLOPMenT OF TraiT-SPeCiFiC SnP MarKerS anD an OPTiMizeD genOTyPing WOrKFLOW FOr MarKer-aSSiSTeD BreeDing in riCe M.S. Dwiyanti, C.J. Dilla-Ermita, M.Y. Reveche, E.S. Tandayu, G.A.F. Cariño, S.L. Carandang, D.N. Lozada, K.L. Titong, C. Dizon, S.M. Thein, J.H. Chin, C. Vera Cruz, B. Collard, M.J. Thomson (Philippines)

P0152

reSearCH FOr iMPrOVeMenT OF aMyLOSe COnTenT On riCe CULTiVarS (Oryza SaTiVa L.) By aDVanCeD BaCKCrOSSing MeTHOD Q. Truong (Vietnam)

a HigH-THrOUgHPUT MarKer SySTeM FOr BreeDing OF aFriCan riCe (Oryza gLaBerriMa) J. Pariasca-Tanaka, M. Lorieux, C. He, S. McCouch, M.J. Thomson, M. Wissuwa (Japan)

P0153

MaPPing OF QUanTiTaTiVe TraiT LOCi FOr COOKing anD eaTing QUaLiTy TraiTS in riCe USing genOTyPing-BySeQUenCing M.D. Asante, J. Spindel, J. Cobb, S.K. Offei, V. Gracen, H. AduDapaah, E.Y. Danquah, A. McClung, R. Bryant, S. McCouch (Ghana)

MOLeCULar MarKer-aSSiSTeD iMPrOVeMenT OF HyBriD riCe R. Sundaram, B.C. Viratktamath, C.N. Neeraja, M.S. Madhav, A.S. Hariprasad, P. Revathi, N. Shobha Rani, G.S. Laha, M.S. Prasad, S.M. Balachandran (India)

P0154

STaBiLiTy anaLySiS OF grain yieLD anD iTS COMPOnenTS TraiTS in LOng DUraTiOn riCe genOTyPeS in Bay iSLanD COnDiTiOnS P.K. Singh, R.K. Gautam, K. Kumar, A. Birah, S.K.Z. Ahmed (India)

P0155

JaPOniCa riCe TranSFOrMaTiOn PiPeLine WiTH THe Bar gene SeLeCTiOn B. Dedicova, C. Bermudez, M. Prias, E. Zuniga, C. Brondani (Colombia)

P0156

riCe TranSFOrMaTiOn WiTH enSiFer aDHaerenS OV14 anD TWO agrOBaCTeriUM STainS eHa105 anD LBa4404 B. Dedicova, E. Zuniga, E. Mullins (Colombia)

P0149

HeTerOSiS in F1 HyBriDS aMOng THree LOCaL anD THree HyV riCe (Oryza SaTiVa L.) VarieTieS Z. Jewel (Philippines)

P0150

eSTiMaTiOn OF THe LOCUS FOr STrOng CULM TraiTS, USing reCiPrOCaL CHrOMOSOMe SegMenT SUBSTiTUTiOn LineS DeriVeD FrOM THe CrOSS BeTWeen riCe VarieTieS, KOSHiHiKari anD TaKanari K. Yamamoto, T. Yamamoto, C. Sugiyama, T. Hirasawa, T. Ookawa (Japan)

96

97





POSTer SeSSiOn

POSTer SeSSiOn

13:30 - 15:00

Poster Area

13:30 - 15:00

Poster Area



Poster No THe PyraMiDing OF riCe inTerSPeCiFiC HyBriD STeriLiTy P0157 geneS anD anaLySiS OF BriDge eFFeCT J. Li, J. Zhou, P. Xu, M. He, Y. Zhang, X. Deng, W. Deng, Y. Yang, Z. Wu, D. Tao (China)

Poster No yieLD COnSTrUCTiOn By MODern HigH-yieLDing CULTiVarS P0163 aT UrUgUay F. Pérez de Vida (Uruguay)

P0158

P0159

P0160

P0161

P0162

CyCLOPeDiC anaLySiS OF riCe geneS inDUCeD By HeaVyiOn BeaM irraDiaTiOn K. Ishii, R. Morita, S. Kogure, T. Shibukawa, Y. Nagamura, T. Abe (Japan) enHanCeD SaCCHariFiCaTiOn OF riCe STraW By COnSTiTUTiVe Or SeneSCenCe-inDUCiBLe eXPreSSiOn OF riCe eXO-gLUCanaSe K. Furukawa, M. Nigorikawa, T. Sonoki, Y. Ito (Japan) SiMULaTiOn OF THe eFFeCT OF aDVanCeD inTerCrOSSing FOr THe DeVeLOPMenT OF a POPULaTiOn FOr HigHreSOLUTiOn QUanTiTaTiVe TraiT LOCUS MaPPing anD Larger geneTiC anD PHenOTyPiC VariaTiOn E. Yamamoto, H. Iwata, T. Tanabata, T. Tanaka, R. Mizobuchi, J.I. Yonemaru, T. Yamamoto, M. Yano (Japan) CO(r) 50 : an irrigaTeD riCe VarieTy WiTH neW PLanT TyPe (nPT) TraiTS FOr inDia S. Rajeswari, S. Robin, K. Mohanasundaram, R. Pushpam, S. Manonmani, D. Malarvizhi, R. Rabindran, S. Suresh, V. Ravichandran, L. Subbalakshmi (India) BreeDing FOr rTSV reSiSTanT TrOPiCaL JaPOniCa riCe USing MarKer aSSiSTeD SeLeCTiOn WiTH eCOBaCKgrOUnD SeLeCTiOn J. SHIM, R. Cabunagan, I. Choi, T. Gideon, W. Ha (Philippines)

98

P0164

geneTiC CHaraCTerizaTiOn OF THe riCe (Oryza SaTiVa L.) gerMPLaSM USeD By THe CHiLean BreeDing PrOgraM. V. Becerra, M. Paredes, E. Gutiérrez, C. Alarcón, J. Ale (Chile)

P0165

DeTeCTiOn OF nOVeL QTLS FOr PLanT HeigHT FrOM a riCe CULTiVar, XinHeigUai. K. Miura, S. Segami, M. Nakamura, M. Ashikari, H. Kitano, Q. Qian, Y. Iwasaki (Japan)

P0166

Fine MaPPing FOr a nOVeL gene FOr SOiL-FUrFaCe rOOTing in riCe (Oryza SaTiVa L.) E. Hanzawa, K. Sasaki, M. Obara, Y. Fukuta, Y. Uga, A. Higashitani, M. Maekawa, T. Sato (Japan)

P0167

MOLeCULar CHaraCTerizaTiOn OF DiSOMiC LineS DeriVeD FrOM Oryza LaTiFOLia DeSV. MOnOSOMiC aLien aDDiTiOn LineS in THe BaCKgrOUnD OF THe eLiTe O. SaTiVa BreeDing Line ir31917-54-3-2 R.B. Angeles-Shim, R.B. Angeles-Shim, R.B. Vinarao, N.T. Quilloy, R.M. Malabanan, M. Balram, K.K. Jena (Philippines) Fine MaPPing OF MaJOr QTLS FOr PerCenTage OF STigMa eXSerTiOn On CHrOMOSOMe 3 in riCe Z. Hua, J.J. Mi, F. Wang, C.J. Liu, R.H. Wang (China)

P0168

P0169

PHenOTyPiC anD MarKer aSSiSTeD BaCKCrOSS BreeDing FOr THe iMPrOVeMenT OF BaSMaTi riCe M. Arif, S.A. Naveed, M. Sabar, K. Aslam, P. Capistrano, C.M. Vera Cruz (Pakistan)

99





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13:30 - 15:00



Poster No P0170

Poster No P0176

P0171

P0172

COMBining aBiLiTy anD HeTerOSiS FOr yieLD anD iTS COMPOnenTS in HyBriD riCe USing CyTOPLaSMiC MaLe STeriLiTy Wa BaSeD SySTeM FOr DeVeLOPing HyBriD riCe in inDOneSia Y. Widyastuti, I. Apri Rumanti, S. Satoto (Indonesia) genOTyPe iDenTiFiCaTiOn OF riCe CMS LineS On 18 CyTOPLaSMiC SOUrCeS VarieD aT POLLen aBOrTiVe CHaraCTeriSTiCS By PCr MarKerS On MTDna G. Zhu, T.T. He, Z.H. Sun, W.H. LI, S.L. Jin, X.L. Tan (China) geneTiC VariaBiLiTy, geneTiC aDVanCe anD COrreLaTiOn FOr yieLD, yieLD COMPOnenTS anD SOMe agrOnOMiC TraiTS in WeeDy riCe (Oryza SaTiVa L. F. SPOnTanea) in Sri LanKa A.P. Bentota, D. Rathnasekera, S.G.J.N. Senanayake, U.I.P. Perera (Sri Lanka)

P0173

aDOPTiOn PerFOrManCe OF irri SUPPOrTeD SUBMergenCe TOLeranT riCe VarieTy in BangLaDeSH M. Rahman, N. Sattar (Bangladesh)

P0174

geneTiC DiSSeCTiOn OF HarVeST inDeX, BiOMaSS anD grain yieLD in riCe S. Zhang, X. He, J. Zhao, Y. Chen, T. Yang, Z. Chen, Y. Cheng, X. Wang, Y. Liao, B. Liu (China)

P0175

yieLD POTenTiaL OF HyBriD riCe in TWO DiFFerenT LOCaTiOnS anD SeaSOnS in inDOneSia S. Suyamto, M. M. Saeri (Indonesia)

100

Poster Area

aPPLiCaTiOn OF MOLeCULar MarKer aSSiSTeD SeLeCTiOn (MaS) in arOMaTiC riCe BreeDing X. Duong (Vietnam)

P0177

TOWarDS THe DeVeLOPMenT OF a HigH-THrOUgHPUT MarKer-aSSiSTeD BaCKCrOSSing (MaBC) SerViCe in irri C. Zamora, K. Titong, C.J. Dilla-Ermita, M.A.Y. Reveche, M.J. Thomson, E. Nissila, J.H. Chin (Philippines)

P0178

examining the leaf blueprint: Screening for traits associated with mesophyll cell architecture in the rice (Oryza sativa) full-length cDna overexpressed (riceFOX) lines R.A. Nepomuceno, J. Chatterjee, A. Mabilangan, R. Coe, J. Dionora, W. Quick (Philippines)

P0179

SWeTHa (nLr40024) - a HigH yieLDing, SHOrT DUraTiOn, LODging, BLaST, anD THerMO TOLeranT riCe VarieTy. R. Pottepalem, Y. Suryanarayana, M. Sreevalli Devi, C.P.D. Rajan, P. Raja Sekhar, P.N. Harathi, U. Vineetha, M. Narayanamma, P. Sudhakar, P. Latha (India)

P0180

neLLOre SOna (nLr3041) - a HigH yieLDing, MeDiUM DUraTiOn, Fine grain anD BLaST TOLeranT riCe VarieTy Y. Suryanarayana, R. Pottepalem, M. Sreevalli Devi, C.P.D. Rajan, P. Raja Sekhar, U. Vineetha, M. Narayanamma (India)

P0181

enHanCing THe SaLiniTy TOLeranCe OF VieTnaMeSe VarieTy aS996 USing MarKer aSSiSTeD BaCKCrOSSing T.N.H. Luu, M.C. Luu, H.H. Le (Vietnam)

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13:30 - 15:00

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Poster No THe DeVeLOPMenT OF riCe VarieTieS aDaPTaBLe TO LOW P0182 niTrOgen COnDiTiOn in KOrea U. Hyun, C. Yang, M. Kim, Y. Won, E. Ahn, E. Jeong, B. Kim (Korea)

Poster No VariaTiOn in DayS TO FLOWering aMOng aCCeSSiOnS OF P0189 Sri LanKan TraDiTiOnaL riCe VarieTy "HOnDeraWaLa" COrreLaTeS WiTH VegeTaTiVe grOWTH anD yieLD S. Geekiyanage, E.U.U. Rathnathunga, K.G.P.B. Karunarathne, S. Senaweera, K. Fernando, N. Dissanayake (Sri Lanka)

P0183

CHaraCTeriSTiCS OF MaLe STeriLe MUTanT DeriVeD FrOM KOrean VarieTy 'SeOLHyangCHaL' Y. Won, C. Yang, E. Ahn, U. Hyun, E. Jeong, J. Kim, J. Lee, I. Choi, B. Kim (Korea)

P0184

THe DeVeLOPMenT OF WHOLe CrOP SiLage riCe in KOrea E. Ahn, C. Yang, Y. Won, U. Hyun, E. Jeong, B. Kim (Korea)

P0185

DeVeLOPMenT OF neW CMS LineS SUiTaBLe FOr TaMiL naDU S. Manonmani, M. Umadevi, R. Pushpam, S. Robin (India)

P0186

eVaLUaTiOn OF geneTiC DiVerSiTy USing SSr MarKerS in nPT LineS DeriVeD FrOM inDiCa X JaPOniCa SUB-SPeCieS CrOSSeS OF riCe (Oryza SaTiVa L.) FOr yieLD anD QUaLiTy S. Sanjay kumar singh, R. Swati Sharma, P. G.K.Koutu (India)

P0187

P0188

eXOMe reSeQUenCing reVeaLS MUTaTiOnS inDUCeD By HeaVy-iOn BeaM WiTH LeTMaX in riCe. R. Morita, H. Takehisa, K. Ishii, Y. Hayashi, S. Kogure, K. Ichinose, H. Tokairin, T. Sato, H. Saito, Y. Okumoto, T. Abe (Japan) CHaraCTerizaTiOn OF SeVen HyBriD riCe ParenTaL LineS BaSeD On QUaLiTaTiVe anD QUanTiTaTiVe TraiT I.W. Mulsanti, R.H. Wening, S. Satoto, I.A. Rumanti (Indonesia)

102

P0190

aSSOCiaTiOn MaPPing in MULTi-ParenT POPULaTiOnS OF riCe TO DeLiMiT genOMiC regiOn COnFerring reSiSTanCe TO BrOWn SPOT R. Chitra, V. Lacorte, N. Bandillo, B.E. Huang, H. Leung (Philippines)

P0191

ParTiCiPaTOry VarieTy SeLeCTiOn TriaLS OF STagnanT anD SUBMergenCe TOLeranCe LineS in FarMerS' FieLDS OF an giang PrOVinCe N. Tran Thi, L. Nguyen Thi Lang, B. Bui Chi Buu, L. Romeo V., R. Russel Reinke, I. Abdelbagi, R. Wassmann (Vietnam)

P0192

ParTiCiPaTOry VarieTy SeLeCTiOn TriaLS OF aCiD SULFaTe SOiL TOLeranT LineS in FarMerS' FieLDS OF HaU giang PrOVinCe T. Bui Phuoc, L. Nguyen Thi, B. Bui Chi, H.A. Pham Thi Thu, L. Romeo V., R. Russel Reinke, I. Abdelbagi, W. Reiner (Vietnam)

P0193

ParTiCiPaTOry VarieTy SeLeCTiOn TriaLS OF SUBMerngene anD STagnange TOLeranT LineS in FarMerS' FieLDS OF Can THO CiTy H. Nguyen Van, L. Nguyen Thi, B. Bui Chi, L. Romeo V., R. Russel, I. Abdelbagi, W. Reiner (Vietnam)

103





POSTer SeSSiOn

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13:30 - 15:00

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13:30 - 15:00

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Poster No P0194

Poster No MaPPing QUanTiTaTiVe TraiT LOCi COnTrOL HeaDing DaTe P0200 anD PLanT HeigHT in riCe UnDer MULTiPLe enVirOnMenTS C.S. Wang, C.H. Su, K.K. Hwu (Taiwan)

PLOiDy-DePenDenT SOMaCLOnaL VariaTiOn anD SUCCeSSFUL aPPLiCaTiOn OF DHS MeTHOD in riCe BreeDing L. Heszky, I. Simon-Kiss, J. Pauk (Hungary)

P0195

FARMERS’ ParTiCiPaTOry VarieTaL SeLeCTiOn FOr aDOPTiOn anD DiFFUSiOn OF riCe VarieTieS in nigeria A.A. Efisue, G.B. Gregorio, G. Akinwale (Philippines)

P0196

aPOMeiOSiS in riCe: a FirST STeP TOWarDS aPOMiXiS D. MIEULET, S. JOLIVET, L. CROMER, L. PEREIRA, M. RIVARD, P. MAYONNOVE, G. DROC, B. COURTOIS, E. GUIDERDONI, R. MERCIER (France)

P0197

THe genOTyPing SerViCeS LaBOraTOry aT irri: HigHTHrOUgHPUT SnP genOTyPing FOr BreeDing aPPLiCaTiOnS in riCe M. Reveche, G. Malitic, N. Vieira, G. Sanchez, C. Dilla-Ermita, K. Titong, E. Tandayu, V. Juanillas, J. Chin, R. Mauleon, E. Nissila, M. Thomson (Philippines)

P0198

reSTOrer anD MainTainer Line iDenTiFiCaTiOn TO DeVeLOP riCe HyBriDS FOr UPLanD COnDiTiOn B.A. VEERESHA, N.G. HANAMARATTI, P.M. SALIMATH, M.B. CHETTI, P. SENGUTTUVEL (India)

P0199

DeTerMinaTiOn OF FerTiLiTy BeHaViOUr in THe THerMO SenSiTiVe geniC MaLe STeriLe Line TS 29 FOr iTS CriTiCaL TeMPeraTUreS anD STageS OF FerTiLiTy aLTeraTiOn D. Kavithamani, S. Robin, S. Manonmani, K. Mohanasundaram (India)

104

P0201

ParTiCiPaTOry VarieTy SeLeCTiOn TriaLS OF SaLT – SUBMergenCe TOLeranT LineS in FarMerS' FieLDS OF MeKOng DeLTa VieTnaM L. Nguyen thi, T.A.M. Bui Phuoc, N. Tran Thi, B.I.N.H. Nguyen Hoang Thai, H.I.E.U. Nguyen Van, B.U.U. Bui Chi, L. Romeo V Labios, R. Russel Reinke, I. Abdelbagi Ismail (Vietnam)

P0202

irri gSL-gaLaXy: DaTa anaLySiS anD FOrMaTTing PLaTFOrM FOr riCe SCienCe V.M. Juanillas, M. Thomson, C.J. Dilla, V.J. Ulat, R. Santos, R. Mauleon (Philippines)

P0203

MarKer aSSiSTeD inTrOgreSSiOn OF Pi-z5 anD Pi-5 geneS inTO SaMBHa MaHSUri (BPT-5204), an eLiTe inDiCa riCe VarieTy FOr BLaST reSiSTanCe P. S.K, K.J. Yashavantha, S. Patil, S. AJ, N.G. Hanamaratti, U.D. Singh, R. Rathour, M. Variar, A.K. Singh, T.R. Sharma (India)

P0204

yieLD POTenTiaL OF riCe (Oryza SaTiVa L.) genOTyPeS grOWn in aerOBiC COnDiTiOn UnDer rainFeD LOWLanD eCOSySTeM S. KUMAR, S.S. SINGH, R. ELANCHEZHIAN, B.P. BHATT, O.N. SINGH, A. KUMAR (India)

P0205

BiOinFOrMaTiC anaLySiS TO aSSeSS FOr POTenTiaL aLLergeniC CrOSS-reaCTiViTy OF PrOTeinS in gOLDen riCe 2 N. Oliva, R. Goodman, G. Barry, I. Slamet-loedin (Philippines)

105





POSTer SeSSiOn

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13:30 - 15:00

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13:30 - 15:00

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Poster No geneTiC DiVerSiTy reLaTeD TO eaTing QUaLiTy in P0206 JaPOniCa riCe gerMPLaSM BaSeD On SnP MarKer anaLySiS Y. Cho, J. Suh, Y. Won, M. Yoon, J. Lee, M. Baek, H. Park, J. Park, W. Kim, S. Baek, K. Ha, J. Lee (Korea)

Poster No gene aCTiOn FOr grain yieLD anD iTS COMPOnenTS in riCe P0212 (Oryza SaTiVa L.) UnDer SODiC SOiL G. Dharwal, O.P. Verma, G.P. Verma, P.K. Singh, D.K. Dwivedi, Y. Kumar, P.K. Yadav, R.R. Tiwari (India) P0213

COMBining aBiLiTy FOr yieLD anD iTS COnTriBUTing COMPOnenTS in riCe (Oryza SaTiVa L.) UnDer SODiC SOiL P.K. Yadav, O.P. Verma, G.P. Verma, P.K. Singh, D.K. Dwivedi, K.K. Mishra, V.K. Mishra, A. Devi (India)

P0214

geneTiC VariaBiLiTy anD COrreLaTiOn STUDieS in inDigenOUS anD eXOTiC geneTiC STOCKS OF riCe (Oryza SaTiVa L.) R. Yadav, O.P. Verma, G.P. Verma, D.K. Dwivedi, P.K. Singh, K. Singh, R.K. Chaudhary, P.K. Yadav, A. Devi, R.R. Tiwari (India)

P0215

COMBining aBiLiTy FOr yieLD anD iTS COMPOnenTS in riCe (Oryza SaTiVa L.) USing CMS SySTeM S. Dwivedi, P.K. Singh, O.P. Verma, A. Devi, R. Dwivedi, D. Dwivedi (India)

STUDieS On HeTerOTiC POTenTiaL in riCe (Oryza SaTiVa L.) FOr yieLD anD iTS COMPOnenTS USing CMS SySTeM S. Dwivedi, P.K. Singh, O.P. Verma, A. Devi, R. Dwivedi, D. Dwivedi (India)

P0216

COrreLaTiOn anaLySiS OF riCe gerMPLaSM anD iTS BreeDing aPPLiCaTiOnS C. Uddaraju, L.V. Subba Rao, G. Shiva Prasad, K. Suneetha, N. Shobha Rani, N.K. Gautam, S. Kalyani (India)

aSSOCiaTiOn OF QUanTiTaTiVe TraiTS in riCe (Oryza SaTiVa L.) UnDer SODiC COnDiTiOn O.P. Verma, A.H. Khan, D. Dwivedi (India)

P0217

yieLD reSPOnSeS OF riCe genOTyPeS FOr DrOUgHT TOLeranCe in rainFeD LOWLanD SiTUaTiOn S. Singh (India)

P0207

iDenTiFiCaTiOn OF riCe TgMS LineS WiTH OUT - CrOSSing FaVOring FLOraL TraiTS R. PUSHPAM, S. Robin, S. Manonmani, M. Umadevi (India)

P0208

HeTerOTiC eFFeCTS On grain yieLD in HyBriD riCe (Oryza SaTiVa L.) UnDer SODiC SOiL U. Verma, O.P. Verma, M. Kumar, G.P. Verma, P.K. Singh, A. Devi, Y. Kumar, P.K. Yadav, S. Dwivedi, R.R. Tiwari, D.K. Dwivedi (India)

P0209

iDenTiFying SUPeriOr FerTiLiTy reSTOrerS anD MainTainerS FOr CMS LineS OF riCe (Oryza SaTiVa L.) UnDer SODiCiTy U. Verma, O.P. Vrema, G.P. Verma, M. Kumar, P.K. Singh, D. Dwivedi (India)

P0210

P0211

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Thursday, november 07, 2013 POSTer SeSSiOn 13:30 - 15:00

Poster Area

BreeDing aPPLiCaTiOnS (contd.) Poster No P0218

eVaLUaTiOn OF THe eFFeCT OF SPr3 LOCUS CaUSing SPreaDing On yieLD-reLaTeD TraiTS in riCe M.E. Fabreag, D.M. Kim, S.M. Yeo, S.N. Ahn (Korea)

P0219

geneTiC ParaMeTer STUDy aMOng riCe PrOMiSing LineS I. Rumanti, I. Mulsanti (Indonesia)

P0220

CUrrenT STaTUS anD FUTUre PrOSPeCTS OF HyBriD riCe in JHarKHanD, inDia M. Pandey, D.N. Singh (India)

P0221

HyBriD riCe reSearCH anD DeVeLOPMenT in egyPT H. El-Mowafi, A.M.R. Reda, R. Abdallah, E. Arafat, O. El-Badawi (Egypt)

P0222

iDenTiFiCaTiOn OF arOMaTiC HyBriD riCe USing MiCrOSaTeLLiTe (SSr) MarKerS in egyPT. H. El-Mowafi, A.M.R. Reda, R. Abdallah (Egypt)

P0223

DeVeLOPMenT OF iMPrOVeD LineS WiTH TOLeranCe TO FLOODing aT gerMinaTiOn STage FOr DireCT SeeDeD riCe eCOSySTeMS C. Casal, J.A. Tarun, J.C. Ignacio, A. Pamplona, D.J. Mackill, A.M. Ismail, E.M. Septiningsih (Philippines)

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ABSTRACTS

1


history of agriculture in Asia. The varied ecologies of annual and perennial forms of wild rice promoted different huntergatherer collecting strategies and early cultivation in parts of the Ganges and Yangtze valleys. The earliest selection for domestication traits can be documented during early millennia of cultivation in the Lower Yangtze, and possibly elsewhere in China. Adaptations involved with the early northward dispersal rice included overcoming constraints of seasonality, and in due course tougher rachises to allow longer grain filling. The diversification of wet versus dry and less intensive systems of cultivation was important as rice spread to Southeast Asia. The development sticky rices and fragrant rices, and changes in the color of grains can all be regarded as adaptations to cultural preferences and highlight regional patterns in the history of rice. Once available many adaptations were spread across rice lineages through hybridization processes, highlighting that the history of rice as with agricultural systems and culture in general is one of reticulate evolution.

PLenary SeSSiOnS

PS01-01 Linking genome wide association studies (gWaS) and plant breeding to better utilize natural variation in rice S. McCouch Cornell University, New York, United States Abstract not available. PS01-02 ecology and Cultural History made rice: adaptive episodes, diversification and reticulation over 8000 years D. Fuller University College London, London, United Kingdom The rice we have today is the product of a long and diverse cultural history in which local ecologies and cultural practices have created varied local selection pressures on the crop. This presentation draws on current archaeological evidence for rice, observations on the ecology of wild and cultivated rices and their associated flora, and genetics, to provide a synthetic history of how rice has diversified and adapted to the varied ecologies and cultural environments through the long

PS02-01 Functional and nutritional traits from wild rices R. Henry Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, Australia Wild crop relatives represent an important reservoir of diversity for crop

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improvement. Wild genetic resources may be a key to developing climate resilient crops. The functional and nutritional qualities of plants have often been subject to significant selection during domestication. New sources of wild germplasm are being characterized at the whole genome level revealing possible new species for use in rice improvement. Analysis of genes associated with starch properties is now possible across the entire Oryza genus. Two strategies have been employed: amplicon sequencing of target genes across large populations and analysis of assembled whole genomes across species. Analysis of starch metabolism genes in rice breeding lines and comparison with wild germplasm suggests that selection for starch associated traits has made significant changes but at relatively few loci. This approach allows rapid incorporation of genes from wild material while maintaining rice quality and suggests options for further improving rice for nutritional and functional purposes.

molecular markers to improve grain yield and other agronomic traits. The following lessons that maize breeders have learned may be of value to breeders of other species. First, the best ways to use markers in plant breeding depend on the genetic architecture of the trait. In particular, QTL introgression is useful for a trait that may have major genes whereas genomewide selection (or genomic selection) is the preferred approach for more complex traits such as grain yield. Second, genomewide selection is most advantageous when heritability is high in the training population but is low or zero in the test population. Third, genomewide predictions are usually most accurate with a simple model that assumes that each marker accounts for an equal proportion of the total genetic variance and that ignores epistasis. Fourth, traits differ in their prediction accuracy even when the marker density, population size, and heritability are kept constant. Fifth, for finding marker-trait associations, models that incorporate genomewide background effects are superior to the K and QK models commonly used for association mapping. Sixth, QTL mapping in biparental crosses has breeder-friendly advantages over association mapping. Seventh, an approach that integrates information on known major genes and polygenic background effects is advantageous when a trait has a mixed mode of inheritance. I will conclude by presenting future needs in applying markers in plant breeding.

PS02-02 What we've learned and are still learning about using molecular markers to improve grain yield in maize R. Bernardo Agronomy and Plant Genetics, University of Minnesota, Saint Paul, USA In the past 25 years, maize breeders have tried different ways of using

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PS02-03

and subsequently adapted these models to select plants with high yield potential in F2 populations. We are currently characterizing the yield potential of the progenies of selected plants. In parallel, we have also embarked on establishing an ideal genome composition of diverse germplasms via genome shuffling using 8 rice cultivars with the aim of developing base populations that could be used in future breeding programs. The current status of genomic selection and progress in population development will be presented.

next-generation breeding in rice: challenges and perspectives 1

1

1

M. Yano , E. Yamamoto , J. Yonemaru , 1 2 2 R. Mizobuchi , H. Kato , K. Matsubara , 2 2 J. Tanaka , S. Kobayashi , H. 2 1 Tsunematsu , T. Yamamoto 1

National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan 2 Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan

PS03-01

Advances in rice genomics have allowed us to explore the potential of markerassisted selection (MAS) in backcross breeding. So far, significant progress has been achieved in introgression breeding using MAS particularly in improving disease and insect resistance and environmental stress tolerance in rice. However, although several individual genetic factors for yield potential such as seed size and number have already been identified, single factor introgression by MAS has not yet been shown to make any major impact in improving yield. For complex traits such as yield potential, it would be necessary to manipulate multiple factors simultaneously in the course of selection. Genomic selection (GS) is a new breeding strategy that may provide the key in maximizing the full potential of MAS. We are currently implementing GS to improve the yield potential in the top cross of Japanese cultivars for forage use. We have developed statistical models for predicting total biomass based on genotype and phenotype information in recombinant inbred lines,

Cloning and application of a series of high-yield genes iPa1/DeP1/gW8 in rice Q. Qian, D. Zeng, G. Zhang, Z. Gao, L. Zhu, J. Hu, J. Xu, G. Dong, X. Hu, D. Xue, L. Guo State Key Lab of Rice Biology, China National Rice Research Institute, China The growing world population and shrinkage of arable land demand yield improvement of super rice, one of the most important staple crops. We used the screened rice mutants and elite germplasm for gene cloning, functional analysis and breeding utilization. A series of rice important functional genes of IPA1, DEP1, and GW8 were cloned. IPA1 (ideal plant architecture 1) a major QTL gene from a japonica rice variety Shaonijin, encoding a transcription factor containing an SBP-box, and could reduce tillers, strengthen stems, and enhance grain number, weight and grain

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yield. Another QTL DEP1 (dense and erect panicle 1) from a japonica rice Shennong265 encoding a PEBP-domain protein could increase grain density, more branches and grains per panicle, and yield by 15–20%. We have conducted the molecular breeding strategy known as QTL polymerization of the yield genes such as GW8/GS3, DEP1/Gn1a, and Gn1a/sd1. In super hybrid rice study, it is important to develop additional methods and concepts for the QTL-based breeding of high-yield rice, including rice restorer lines with ideal plant architecture, such as the large-panicle IPA1/Ghd8/Gn1a line (9311- IPA1/Ghd8/Gn1a), and sterility lines with DEP1/Ghd7 /GS3 large panicles with inter-subspecies heterosis. The use of inter-subspecies heterosis and the design breeding of plant ideotypes with large panicles will be beneficial to fulfilling new yield leap in rice.

they are one of the key components required for protein translation and, consequently, for cell growth and development. Ribosome biosynthesis requires the synchronized activity of the three RNA polymerase systems: RNA pol I and III transcribe the ribosomal 45S (18S, 5.8S and 25S) and 5S rRNA, respectively, while RNA pol II transcribes mRNA encoding the 80 ribosomal proteins that assemble with rRNAs to form large and small ribosome subunits. The haploid genome of Arabidopsis thaliana is approximately 146 Mbp distributed over 5 chromosomes. The 45S rDNA gene units are present in ~750 copies grouped at the top of chromosomes 2 and 4. For the 5S rDNA genes, ~1000 copies occur in long tandem arrays localized in the pericentromeric heterochromatin of chromosomes 3, 4 and 5. In contrast, the ~240 genes encoding the ribosomal proteins are distributed in duplicated region of all 5 chromosomes.

PS03-02

Regulation of RNA pol I transcription is an important mechanism in controlling ribosome abundance. In actively growing cells, ribosomal RNA synthesis represents about 50% of total cellular RNA production. The first steps of 45S rRNA synthesis require the concerted and coordinated activity of two 45S rRNA making machineries: the RNA polymerase I holoenzyme and the U3nucleolin processing complex.

rna Molecular Machines and Protein Making Factories J. Sáez-Vásquez, F. Pontvianne, N. Durut, M. Abou-Ellail, C. Charbonnel, P. Comella, A. DeBures, E. Jobet, R. Cooke Laboratoire Génome et Développement des Plantes, France

Biochemical and genetic characterization of these RNP complexes and other aspects of global rDNA gene organization and expression will be presented.

The nucleolus is a multifunctional nuclear structure in which ribosome assembly takes place. Production of ribosomes, the cellular protein making factories, is a major cellular activity as

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PS04-01

Several BPH resistance genes in rice have been isolated and characterized using map-based cloning, providing a means for understanding the molecular basis for rice resistance to BPHs. The Bph14 gene encodes an R protein of the NB–LRR family. Bph9 is another BPH resistance gene encoding NB–LRR protein. Bph18 gene encodes a varied R protein lacking LRR domain (Jena, 2010). These R genes function in BPH resistance through an effector-triggered immunity. Another type of BPH resistance gene is exemplified by the OsLecRK, a gene encoding for a receptor-like kinase which we cloned at Bph15 locus. OslecRK has been characterized as a crucial role in resistance to BPH, blast and bacterial blight. OslecRK is considered as a pattern receptor conferring BPH resistance in rice.

engineering a C4 rice W.P. Quick International Rice Research Institute (IRRI), Los Baños, Philippines Abstract not available. PS04-02 Characterization of brown planthopper resistance genes in rice G. He, B. Du, R. Chen, S. Jing, L. Zhu State Key laboratory of Hybrid Rice, Wuhan University, Wuchang, Wuhan, China The brown planthopper (BPH) is the most notorious insect pest of rice. Growing resistant rice varieties is the first option for controlling the pest. Since the Bph1 was first identified in rice, a total of 28 genes for BPH resistance have been reported. 13 of the BPH resistance genes were identified in cultivated rice, and 15 identified in rice lines descent from wild rice hybridization programs. 22 BPH resistance genes have been mapped on rice chromosomes. Interestingly, these genes are found to cluster on 4 rice chromosomes. In summary, 8 BPH resistance genes cluster on chromosome 12 , 7 on chromosome 4, 4 on chromosome 3 and 3 on chromosome 6. The results suggest that BPH resistance genes have evolved at particular loci in rice genome.

PS04-03 genetic improvement of root system architectures to enhance drought avoidance in rice Y. Uga National Institute of Agrobiological Sciences, Japan Drought is the most serious abiotic stress that hinders rice production under rainfed conditions. Lowland rice is susceptible to drought stress because of its shallow rooting compared with upland rice and therefore a limited capacity to extract water from deep soil layers. To enhance drought avoidance in lowland rice, genetic improvement of root system

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Faculty of Life Sciences, Toyo University, Japan

architecture by using a marker-assisted selection (MAS) is considered one of the most promising breeding strategies. We isolated a deeper rooting QTL, DRO1, by using map-based cloning strategy in a mapping populations derived from a cross between IR64, a lowland rice with shallow roots and Kinandang Patong (KP), an upland rice with deep roots. A near-isogenic line for DRO1 (Dro1-NIL) in the IR64 genetic background increased deep roots, resulting in drought avoidance compared to IR64. For further improvement of drought avoidance in IR64, using MAS pyramiding, we are developing IR64 having KP allele for DRO1, QTLs for root length (qRL6.1), root thickness (Sta1), and root volume (qFSR4). To improve root system architecture in other shallow-rooting varieties except for IR64, we made different F2 populations derived from crosses between three different shallow-rooting varieties and KP, and analyzed QTLs for deep rooting in these populations. As a result, we detected a major deep-rooting QTL, DRO2, on chromosome 4. Currently, we push forward simultaneously cloning of DRO2 and developing of NILs. These NIL series will be promising materials for molecular breeding of root system architectures in the future.

Increases in the yield of rice, a staple crop for more than half of the global population, are imperative to support rapid population growth. Grain weight is a major determining factor of yield. We had cloned TGW6 (THOUSAND GRAIN WEIGHT 6), a gene from the Indian landrace rice ‘Kasalath’, and analyzed its function. TGW6 encodes a novel protein with IAA-glucose hydrolase activity, and 1-bp deletion caused a frame shift and prevented the production of the mature protein due to a premature stop codon in ‘Kasalath’ allele. In sink organs, ‘Nipponbare’ tgw6 expressed in developping endosperm and affected the timing of the transition from the syncytial to the cellular phase by controlling IAA supply, and consequently limited cell number and grain length. Most importantly, the loss of function in the ‘Kasalath’ allele has desirable consequences, increased cell numbers in endosperm and grain length. Through pleiotropic effects on source organs, ‘Kasalath’ allele TGW6 enhances grain weight, leading to significant yield increases. We found the ‘Kasalath’-type TGW6 only in one O. rufipogon line and scattered across the genotypes of four cultivars from Indochina. These suggest that TGW6 might be a gene left behind in domestication that can be useful for further improvements in yield characters in modern cultivars.

PS05-01 The genetic control of rice yield through gleaning superior genes N. Hirotsu

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PS05-02

PS05-03

Bacterial blight of rice: a window to evolution, disease and everything else

genomics-assisted transfer of QTLs for tolerance to drought, flooding and salinity in to indian mega rice varieties

F. White

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N.K. Singh , R. Singh , Y. Singh , N. 1 2 3 Yadav , A.K. Singh , P.C. Sharma , S.L. 3 3 Krishnamurthy , S.K. Sharma , J.L. 4 4 4 Dwivedi , A.K. Singh , V.N. Singh , J.S. 4 4 5 Verma , S.P. Singh , P.K. Singh , J. 5 6 4 Singh , R. Kumar , Nilanjay , N.K. 4 7 7 8 Singh , T. Ahmad , S.K. Chetia , M. Rai , 9 10 10 R. Perraju , D.N. Singh , A. Pandey , T. 11 11 Mohapatra , N.P. Mandal , J.N. 11 11 11 Reddy , O.N. Singh , J.L. Katara , B. 11 11 11 Marandi , P. Sawain , R.K. Sarkar , 11 12 13 D.P. Singh , S. Verulkar , T. Ram , G. 13 14 Padmawathi , Y. Suraynarayana , P.R. 14 14 14 Rao , M.G. Rani , T. Anuradha , R.M. 15 15 Kathiresan , A. Anandan , S. 16 16 Thirumeni , K. Paramsivam , S. 16 17 Nadarajan , A. Kumar , E. 17 17 Septiningshih , U.S. Singh , U.S. 17 Ismail

Department of Plant Plant Pathology, Kansas State University, Manhattan, Kansas, USA Xanthomonas oryzae pv. oryzae (Xoo) causes the disease bacterial blight of rice. Of the various susceptibility and resistance factors that have been characterized in the interaction of Xoo and rice, many involve a class of bacterial virulence factors known as the transcription activator-like (TAL) type III effectors. Gene expression profiling and functional analysis has revealed a variety of host genes and pathogen virulence factors that have critical contributions to the outcome of the host pathogen interaction. TAL effectors have major effects on susceptibility, and the rice, evolutionarily, has adapted to include an assortment of novel genetic controls for resistance. All strains of Xoo have a requirement for the induction of at least one member of the SWEET, gene family. Other host induced genes are predicted to function in inducing ecotopic host small RNA processing, enzyme induction, and gene regulation. On the host side, host genotypes exist that alter the effectiveness of TALmediated susceptibility, involving both dominant and recessive mechanisms.

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National Research Centre on Plant Biotechnology, New Delhi, India 2 Indian Agricultural Research Institute, New Delhi, India 3 Central Soil Salinity Research Institute, Karnal (Haryana), India 4 Acharya Narendra Dev University of Agriculture and Technology, Faizabad (UP), India 5 Banaras Hindu University, Varanasi (UP), India 6 Rajendra Agricultural University, Samastipur (Bihar), India 7 Asam Agricultural Univerrsity, Jorhat (Assam), India 8 Central Agricultural University, Umiam (Meghalaya), India

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5 - 8 November 2013 � Dusit Thani Hotel, Manila, Philippines 9

Jawahar Lal Nehru Krishi Vishwavidyalay, Reewa (MP), India 10 Birsa Agricultural University, Ranchi (Jharkhand), India 11 Central Rice Research Institute, Cuttack (Odisha), India 12 Indira Gandhi Agricultural University, Raipur (Chhatishgarh) 13 Directorate of Rice Research, Hyderabad, India 14 Acharya N.G. Ranga Agricultural University, Maruteru (AP), India 15 Annamalai University, Annamalai Nagar (TN), India 16 PAJANCOA, Karikal (Puduchery), India 17 International Rice Research Institute (IRRI), Los Baños, Philippines Rice production in rainfed areas in India is low because of too little or too much rain, or salinity. Breeding rice varieties with inbuilt tolerance to these abiotic stresses offers an economically feasible solution to improving rice productivity in these areas. Inspired by the success of recently-released rice variety ‘SwarnaSub1’ by IRRI-India collaborative efforts, Indian Government Department of Biotechnology started a major molecular breeding programme in technical collaboration with IRRI’s STRASA project to transfer five major QTLs for grain yield under drought (qDTY1.1, qDTY2.1, qDTY3.1, qDTY9.1, qDTY12.1) and one QTL each for submergence (Sub1A1 gene) and salinity (Saltol) into a number of popular Indian rice varieties. The approach being followed is to transfer only a small segment of the donor genome by applying rigorous foreground, recombinant and background selection to develop nearisogenic lines with minimum affect on

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quality and adaptation of the original varieties. Foreground selection using tightly-linked or gene-based functional markers is being conducted at each generation with emphasis on elimination of linkage drag during the BC1 and BC2. The Saltol QTL interval was further narrowed down during genomicsassisted backcross breeding programme due to its transfer in to a number of genetic backgrounds and coupling of phenotypic screening with DNA marker based foreground selection. A highdensity SNP panel will be used for background selection after BC2 generation to select the best NIL derivatives from a set of lines identified by phenotypic background selection during BC1 and BC2. PS06-01 MagiC populations for genetic research and breeding applications 1

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H. Leung , N. Bandillo , C. Raghavan , 2 1 1 E. Huang , R. Mauleon , M. Thomson , 1 1 1 E. Redona , R.K. Singh , G. Gregorio 1

Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute (IRRI), Los Baños, Philippines 2 Mathematics, Informatics and Statistics, CSIRO, Brisbane, Queensland, Australia advanced MAGIC (Multi-parent inter-Crosses) is an generation experimental method to create highly recombined germplasm for direct use as breeding lines and for fine mapping of QTL. The increased recombination in MAGIC populations can lead to novel

PS06-02

rearrangements of alleles and greater genotypic diversity for broader adaptation. Sixteen improved varieties carrying desirable agronomic traits were used as founder parents to produce 4 multi-parent populations: indica MAGIC (8 indica); MAGIC plus (2 additional rounds of inter-crossing); japonica MAGIC (8 japonica); and Global MAGIC (intercross between 8-way F1s of the indica and japonica MAGIC). Genotyping-by-sequencing was used to generate ~17,000 SNP markers for genome-wide association analysis of QTL for blast and bacterial blight resistance, salinity and submergence tolerance, and grain quality. Depending on genomic regions, QTL were mapped with a resolution between 6 to 0.13 Mb. Linkage blocks of Indica MAGIC and MAGIC PLUS lines were examined to determine whether extra inter-crossings lead to increased recombination. Field evaluation of indica MAGIC lines at multiple locations in the Philippines (3), Tanzania (1), and Vietnam (1) showed transgressive segregation in yield, leading to the identification of lines with stable high yield across locations. Our results suggest that MAGIC populations can potentially serve multiple purposes: as breeding materials in breeding programs, for mapping multiple QTL simultaneously, and supporting QTL detected by GWAS of diverse germplasm. Additional MAGIC populations are being produced to create new gene combinations for tolerance to multiple diseases, insects, and heat stress.

Molecular dissection of rice innate immunity to the blast fungus Magnaporthe oryzae G. Wang

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Department of Plant Pathology, Ohio State University, Ohio, United States 2 Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the main limitations in rice production worldwide. Although many resistance (R) genes in rice and avirulence (Avr) genes in M. oryzae have been cloned in the last two decades, the molecular mechanism of the rice and M. oryzae interaction has not been fully understood. In the last few years, we have applied an integrated approach to dissection of the rice innate immunity and identified several key genes that play important roles in interacting with fungal effectors and regulating defense signaling in rice. After we cloned the R gene Piz-t in rice and the cognate Avr gene AvrPiz-t in M. oryzae, we started to analyze the function of the 3 AvrPiz-t-interacting protein (APIPs) genes in rice defense. We found that the E3 ligase gene APIP6 is a positive regulator of PAMP-triggered immunity (PTI), the E3 ligase gene APIP10 is not only a positive regulator of PTI but also a negative regulator of Pizt-mediated resistance and the transcription factor gene APIP5 negatively regulates cell death and disease resistance to M. oryzae. In our effort to dissection the SPL11-mediated

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defense pathway, we demonstrated that SPIN6 interacts with the U-box E3 ligase and negatively regulate cell death and defense. We also found that the histone H4 deacetylase gene HDT701 negatively regulates the resistance to both M. oryzae and Xamthomonas oryzae pv. oryzae by modulating histone H4 acetylation of defense-related genes, suggesting the importance of epigenetic regulations in rice immunity.

genomes. To eliminate this limitation we have been developing sequencing strategies and assembly improvements to carry out true de novo assemblies of Nipponbare (as a control), IR64 and a strain of rice from the aus group. This approach finds differences that are not seen in reference guided reassemblies. These biological differences that are likely to result from some of these differences will be discussed. Lastly, combining new technology from Pac Bio with Illumina allows us to increase the N50 contig size of the de novo assemblies several fold; to about 150kb, and strategies for continued improvement of N50 size and the resulting improved resolution of structurally complex regions of the genome will be described.

PS06-03 new whole genome de-novo assemblies of three divergent strains of rice document novel gene space in aus and indica missed by previous reference assemblies R.W. McCombie


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PS07-01

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Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, United States 2 Rice Sequencing Consortium, Cornell University, New York, United States

impacts of rising temperatures on rgene-mediated resistance in rice 1

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J.E. Leach , R. Corral , H. Liu , S.K. 2 3 3 Srivastava , J.F. Balidon , I.R. Choi , S. 5 3 1 Kikuchi , R. Mauleon , C. Jahn , A. 2 3 1,4 Pereira , C. Vera Cruz , V. Verdier

Differences among the genomes of rice strains contribute to their salient biological properties including encoding variation in important agronomic traits. Technologies for sequence analysis of the divergent strains typically rely on mapping back to a reference genome, typically the Nipponbare genome in the case of rice. This is especially true for very low cost next gen sequencing approaches. While this type of next gen sequencing provides much useful information, the nature of the algorithms used for the alignments to the reference genome tend to filter out significant structural rearrangement between

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Colorado State University, Bioagricultural Sciences, Plant Sciences, Fort Collins, Colorado, United States 2 Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, Arkansas, United States 3 International Rice Research Institute (IRRI), Los Baños, Philippines 4 Institut de Recherche pour le Développement, IRD-CIRAD-UM2, Montpellier, France

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National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan

stresses, and will guide future strategies to mitigate the impacts of climate change.

The severity of many plant diseases is predicted to increase as environmental temperatures rise. Unfortunately, many widely used disease resistance (R) genes are less effective at high temperatures. In rice, high temperatures are conducive to bacterial blight (BB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo). Most BB R genes, including Xa4, xa5, Xa10, xa13, are less effective at controlling disease at high relative to normal temperature regimes. In contrast, rice BB R gene Xa7 restricts disease more effectively at high than low temperatures, and this is associated with reduced lesion lengths, bacterial spread, and bacterial numbers. Xa7 recognizes the Xoo AvrXa7 virulence effector that activates expression of a rice sucrose transporter OsSWEET14, among other susceptibility genes. To understand the molecular impacts of the interactions of biotic (disease and resistance) and abiotic (temperature) stresses on rice, we compared transcriptomes from X. oryzae-rice interactions involving avrXa7-Xa7 interactions at high and low temperatures. In general, more genes exhibited altered expression (up or down) at high than low temperatures. Certain pathways, such as the light reactions, terpene metabolism, and the Calvin cycle, were up-regulated at high relative to low temperatures during resistant interactions, while hormone biosynthesis genes were downregulated by high temperature. Understanding the increased efficacy of Xa7 at high temperatures will provide insights into the dynamic cellular changes to combined biotic and abiotic

PS07-02 15 Million years of Oryza evolution as Seen Through the analysis of 12 High-Quality genome assemblies R. Wing Arizona Genomics Institute, University of Arizona, United States The genus Oryza contains 23 species (2 cultivated and 21 wild), encompasses ~15 million years of diverse evolutionary history, and contains a virtually untapped reservoir of genes that can be used to improve cultivated rice. The International Oryza Map Alignment Project formed in 2007 at the ISRFG meeting in Tsukuba, Japan and set 3 major goals: 1) Generate RefSeqs & Transcriptome data sets for all eight AA genome species, and a representative species of the nine other genome types; 2) Generate, map, and phenotype advanced ABC, CSSL, RIL populations for the AA genome species for functional and breeding studies; and 3) Identify collections of naturally occurring populations of the wild Oryza species for diversity, conservation, population and evolutionary analyses. Recently, IOMAP completed the sequencing, assembly (genomic and transcriptomic), and annotation of 9 Oryza genomes and the outgroup Leersia perrieri. When this data set is combined with the previously published O. sativa ssp. japonica and indica genomes, we now a total 11 high-

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quality Oryza genomes available for interrogaton. My talk will discuss the generation and analysis of this 12 genome data package and how we can used this resource to help solve the 9billion people question – i.e. How can grow enough food to feed 2 billion additional human inhabitants in less than 40 years.

present study, we show that a simple morphological change in panicle shape, controlled by the SPR3 locus, had a large impact on seed-shattering and pollinating behavior. In the genetic background of wild rice, we found that plants with a cultivated type of closed panicle had significantly reduced seed shattering through seed retention. Additionally, their long awns in closed panicles disturbed free exposure of anthers and stigmas on the flowering spikelets, resulting in a significant reduction of the outcrossing rate. The SPR3 locus was localized in a 9.3-kb genomic region, and a complementation test suggested that this region regulates the liguleless gene (OsLG1). A sequence analysis revealed a selective sweep around the SPR3 genomic region in cultivated rice. These results demonstrate that a closed panicle was the selected trait in the early phase of rice domestication and that rice was destined to become a self-pollinating crop.

CS01-01 Closed panicles in rice domestication 1

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T. Ishii , K. Numaguchi , K. Miura , K. 4, 5 1 1 Yoshida , T.T. Pham , T.M. Htun , M. 1 6 4 Yamasaki , T. Matsumoto , R. Terauchi , 1 2 R. Ishikawa , M. Ashikari

PS07-03

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rice diversity and genetics for future plant breeding

Graduate School of Agricultural Science, Kobe University, Kobe, Japan 2 Bioscience and Biotechnology Center, Nagoya University, Nagoya, Japan 3 Faculty of Biotechnology, Fukui Prefectural University, Yoshida, Japan 4 Iwate Biotechnology Institute, Kitakami, Japan 5 The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom 6 National Institute of Agrobiological Sciences, Tsukuba, Japan

R.S. Zeigler International Rice Research Institute (IRRI), Los Baños, Philippines Abstract not available.

Reduction in seed shattering was an important phenotypic change during cereal domestication. In rice (Oryza sativa L.), two loci, qSH1 and sh4, have a strong influence on the seedshattering habit. The wild progenitor O. rufipogon Griff. has functional alleles at both loci that enhance the formation and development of an abscission layer between the pedicel and spikelet. However, each of the non-functional cultivated alleles does not contribute to the non-shattering phenotype in the genetic background of wild rice. The key gene or trait that suppressed seed shattering during the early phase of rice domestication is unknown. In the

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CS01-02 in Search of vestige of artificial selection in rice 1

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J. Lyu , S. Zhang , W. He , J. Zhang , F. 2 1 Hu , W. Wang 1

CAS-Max Planck Junior Research Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2 Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan, China

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BGI-Shenzhen, Shenzhen, Guangdong, China

Domestication and improvement are two important artificial selection processes which give rise to our rich rice germplasm resources. Studying genomic changes under artificial selection can not only reveal the interesting stories about rice breeding in the past, but also provide great insights for us to further improve our varieties. Our work focused on genomic changes under selection during rice domestication and improvement. To study rice domestication, we resequenced the genomes of 25 wild accessions and 25 cultivars. For the topic of improvement, we used an elite variety tag SNP (ETAS) approach and deep-sequencing data to investigate genes contributing to the elite performance of improved varieties. And also we used a comparative genomics approach to study the genetic mechanisms of upland adaptation in rice by sequencing 84 upland and 82 irrigated accessions. Our study for wild and cultivated rice populations supports the dual domestication hypothesis and provides a list of candidate genes for rice domestication. Our study for elite rice provides a new item (ETAS analysis) for the proverbial toolbox of identifying agronomic genes, and we used a comprehensive case study to show the efficiency of the ETAS analysis. Our study about upland adaptation reveals interesting genes potentially related with dry land adaptation. Based on large amount of population genomics data, using evolutionary genetics, comparative genomics, and ETAS approaches, we identified genes potentially associated

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with rice domestication and improvements. Functional data are needed to validate the functional genes under artificial selection and help understand the selection process. CS01-03 Bayesian multi-phenotype genomewide association methods for experimental designs of arbitrary complexity 1, 2

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A.J. Greenberg , J. Mezey , J. 3 1 Jannink , S. McCouch 1

Dept. Plant Breeding & Genetics, Cornell University, Ithaca, New York, United States 2 Dept. Biological Statistics & Computational Biology, Cornell Univ., Ithaca, New York, United States 3 USDA-ARS, Robert W. Holley Center, Ithaca, New York, United States Genome-wide association studies (GWAS) in rice serve as an important starting point for breeding programs. To maximize their potential, it is necessary to evaluate phenotypes that are relevant in target field environments. Thus, complex experiments with a large environmental influence are often required. However, these experiments produce noisy data that may obscure the contribution of genetic variation. Most modern statistical methods for GWAS have been developed to map human disease loci, where each geneticallydistinct individual is evaluated only once and the confounding effects of population structure are not strong. These methods are not adequate for

Rice Research Institute and 146 O. rufipogon accessions. The accessions were analyzed with 70 microsatellite markers, 20 unlinked sequence tagged sites (STS) and 3 chloroplast loci. Microsatellite analysis separated the 416 rice accessions into 4 clusters corresponding to the ancestral groups; Indica, Japonica, Aromatic and Aus. Majority of the rice accessions analyzed were belonged to Indica and were further divided into sub-clusters. Two clusters were evident in the Aus also. O. rufipogon accessions were resolved into 4 groups indicating a strong population genetic structure for this gene pool. One cluster of O. rufipogon was found associated with Indica while another with Aus. Analysis of a subset of rice and O. rufipogon accessions using STS and chloroplast loci supported the phylogenetic interrelationships revealed by the microsatellite data. Here we report a comprehensive study on the genetic structure and phylogenetic relationships of primary rice gene pool in India. Four ancestral groups of rice are found to occur in India but with a distinct geographic distribution for Aus and Japonica. Results revealed a close phylogenetic affinity between certain populations of O. rufipogon and Indica and Aus, providing a new insight into the evolution of cultivated rice in India.

most applications in rice and other crop species. We have developed a general Bayesian approach that accounts for noise in the data, including low heritability, unbalanced designs, missingness, outlier observations, and systematic experimental bias. We also implemented steps to correct for deep population structure (e.g., differentiation between O. s. indica and japonica), and analyze multiple phenotypes at once. We illustrate the advantages of our methods on simulated data and on a set of agronomic phenotypes measured on a diversity panel of rice accessions. CS01-04 genetic structure of cultivated rice (Oryza sativa L.) and its progenitor, O. rufipogon in india 1,2

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D. Raj R , M. Jose , G. Varghese , P. 3 3 4 1 BC , S. ON , R. JC , G. Thomas 1

Plant Molecular Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India 2 Present address: Bishop Moore College, Mavelikara, Alapuzha, Kerala, India 3 Division of Crop Improvement, Central Rice Research Institute, Cuttak, India 4 National Bureau of Plant Genetic Resources, Regional Station, Phagli, Shimla, India

CS01-05

India is rich in the genetic diversity of rice. However little is known on the genetic structure and phylogenetic interrelationships of rice gene pool in India. We used 416 rice accessions consisting of 353 Indian accessions and 63 reference cultivars from International

Origin and ecotypic differentiation of Bangladeshi indica rice 1

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N. Hoque , S. Begum , M. Islam , G. 2 Gregorio

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gene diversity excess. Marker trait association was significant for marker loci related to grain- size/shape, shattering, fragrance, number, stickiness and pericarp color gene(s). Selected subset of O. ruphipogon, Laxmidigha, Maliabhangor and Jagliboro comprised alleles from total population. Population diversity and structure of Bangladeshi rice explain to the domestication process as Oryza ruphipogon first given rise to intergrades (Wild4326 & Wild4855) which consequently differentiated into Jagliboro, Laxmidigha, Dular, Aswina and Godailaki with an equal probability of sharing gene pool from aus or deepwater aman.

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Biotechnology Division, Bangladesh Institute of Nuclear Agriculture, Bangladesh 2 Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute (IRRI), Los Baños, Philippines Cultivated indica rice at some stage in domestication from wild, evolved quantitative traits like grain-shattering, pericarp color, stickiness, size/shape, fragrance, number, weight/width controlled by genes Sh4, qSH1, Rc, Waxy, GS3, BAD2, Gn1a and GW2. Bangladeshi rice gene pool is at risk by habitat fragmentation and isolation leading to extinction, and its origin is yet to be answered. Purposes of the study were to understand genetic diversity, population structure and distribution of indica rice ecotypes. Oryza ruphipogon, Oryza nivara, intergrades (Wild4326, Wild4855), deepwater-broadcast aman (Maliabhangoir, Aswina, Laxmidigha, Godailaki), aus (Dular) and boro (Jagli) rice were genotyped at 46 SSR loci associated with domestication traits. Grain and molecular marker data were utilized for population genetic analysis, marker trait association and subset selection. SSR marker with tri repeat motif (CAA) resulted highest phylogenetic information. Admixture model by STRUCTURE grouped the rice accessions into four clusters: wild; intergrades, deepwater aman; wild, intergrades, broadcast aman and aus, boro, admixture. Stepwise mutation pattern in SSR markers revealed evidence of bottleneck in population for

CS01-06 Phylogenetic relationship among weedy, wild and cultivated species of Sri Lankan rice based on morphological traits D. Ratnasekera, I.DP. Gangodawatta Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Kamburupitiya, Sri Lanka Rice has been cultivated as a major crop for about hundreds of years in Sri Lanka. Pre-historic to present, existence of rice is evident in Sri Lanka by the presence of endemic cultivars, wild species and land races, improved varieties and weedy forms of rice. In Sri Lanka, five wild species of Oryza, O. nivara, O. rufipogon, O. eichingeri, O. rhyzomatis and O. granulata are known. Improved varieties developed by rice research

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institutes are commonly and extensively grown with few traditional cultivars and weedy forms of rice co-exist frequently in rice eco-systems. In this study we morphologically characterized Sri Lankan wild rice species, selected traditional cultivars, selected improved varieties along with weedy eco-types which were collected island wide to determine the phylogenetic relationship of weedy rice in relation to wild and cultivated rice species. During the field survey, a large number of weedy rice individuals from rice-planting areas across the country were collected. Additionally, a wide range of improved varieties, traditional cultivars and five wild rice species collected from natural habitats were assembled and evaluated in a common field according to the standards of IRRI. Cluster analysis revealed that weedy rice was significantly different from wild rice and closely related to the improved and traditional varieties. When consider the all sub clusters, weedy rice was more close to the improved varieties. The morphological traits that contributed to significant difference were seedling height, leaf length, leaf width, culm length and diameter, plant height, panicle length, grain length, grain width and 100-grain weight.

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N. Ahmadi , C. Billot , G. Droc , D. 3 1 Brunel , J. Frouin , A. 2 4 Ramanantsoanirina , K. McNally , B. 1 1 Courtois , J. Glaszmann 1

CIRAD, UMR AGAP, F-34398 Montpellier, France 2 FOFIFA, Ampandrianomby, Antananarivo, Madagascar 3 Centre National de Génotypage, France 4 International Rice Research Institute (IRRI), Los Baños, Philippines Madagascar Island was one of the last major Old World areas where human settlement was accompanied by the introduction of Oryza sativa. Early studies had reported the presence of a rice group specific to Madagascar. Using 1536 SNP markers, we compared diversity patterns between a panel of 147 Malagasy rice varieties, a reference panel of 370 Asian varieties and representatives of wild relatives of O. sativa. Migration bottleneck has resulted in 30-40% reduction of diversity among the indica and japonica groups in Madagascar. The Malagasy panel showed many fewer indica x japonica recombinations compared to the Asian panel, suggesting that the two groups had undergone much less recombinations when migration to the Island occurred. The existence of the Malagasy-specific group (Gm) was confirmed. Its diversity patterns positioned it halfway from indica and aus groups. Madagascar also hosted cold tolerant tropical japonica varieties, with very long grain. The Gm group most probably arose from founder effect from intermediary forms of rice originated from either India or Sri Lanka that did

CS01-07 Patterns of rice diversity from SnP delineated the origin of the atypical O. sativa group in Madagascar from intermediary forms of the indian subcontinent

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not belong to the four majors O. sativa groups. It then underwent human selection for cold tolerance. Signs of inter-group recombinations were also observed, but recombinations did not seem to have played a major role in the dynamics of rice adaptation to the Island’s agro-ecological constraints. Connections between Gm and O. rufipogon from its putative area of origin reinforce the hypothesis of multiple and diffuse domestication of O. sativa as opposed to two independent domestications occurring in two distinct geographical areas.

India’s forex earning has increased from USD 55 million in 1990’s to USD 3.33 billion in 2012-13, 75% of which is contributed by IARI varieties. Rapid advances in molecular techniques have helped in development of robust markers and their effective utilization in crop improvement. In Basmati rice, novel QTLs for yield traits have been mapped and novel alleles for yield contributing characters such as grain length including a 342 bp deletion in GS3 locus in short grain aromatic rices, width and weight have been identified. Additionally, MAS has been successfully utilized for introgression of gene(s) governing resistance to various biotic stresses such as bacterial blight, blast, brown plant hopper and tolerance to abiotic stresses including salinity, submergence, drought and phosphorus uptake resulting in the development of improved Basmati varieties with resistance to bacterial blight (Improved Pusa Basmati 1), blast resistance (Pusa Sugandh 6), and improved parental lines of Pusa RH 10 with resistance to BB (Pusa 1601, Pusa 1605) and blast (Pusa 1602, Pusa 1603, Pusa 1609). Further, MAS is used in maintenance breeding, testing seed purity in hybrids, and helped in refining and re-orienting the strategy for designing Basmati rice with higher per unit productivity.

CS02-01 Towards improvement of yield potential in Basmati rice: from mapping to marker assisted selection 1

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A. Singh , S. Gopala Krishnan , P. 1 1 2 Bhowmick , K. Prabhu , T. Mohapatra , 2 2 1 N. Singh , T. Sharma , M. Nagarajan , K. 1 Vinod 1

Division of Genetics, Indian Agricultural Research Institute, New Delhi, India 2 National Research Centre on Plant Biotechnology, Pusa campus, New Delhi, India Basmati rice from Indian subcontinent is one of the most popular premium rice known for its unique quality. Systematic efforts for its genetic improvement at Indian Agricultural Research Institute (IARI), New Delhi has enhanced the average per hectare productivity from 2.0 to more than 5.0 tons in the improved Basmati varieties. As a result,

CS02-02 identification of a gene involving in rice grain yield loss by air pollution gas, ozone M. Tamaoki

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caused suppression of APO1 transcription through increase of JA, JAIle, and/or ABA triggered by ozone exposure.

National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan Ozone is the main photochemical oxidant that causes leaf damage, and exposure to it can decrease the productivity of crops. The tropospheric ozone concentration will continue to increase in Eastern Asia, and crop yield decrease by up to 60% in the area. However, the mechanism of rice yield loss by ozone remains unclear. In this study, we performed QTL analysis using chromosome segment substitution lines from Sasanishiki (no effect for grain yield by ozone) and Habataki (decrease grain yield by ozone) to identify a gene involving in rice grain yield loss by ozone. Ozone-exposed Habataki showed decrease of grain yield as well as primary rachis number. The QTL analysis showed a locus located on chromosome 6, which includes aberrant panicle organization 1 (APO1) gene, regulated grain yield loss by ozone. We found the APO1 is the gene that affects grain yield loss in ozone-exposed rice by use of near-isogenic line SHA422-1.1. The transcript levels of APO1 gene in early influorescence meristem decreased remarkably in Habataki and increased in Sasanishiki by ozone exposure. We assumed phytohormones may involved in the signal transduction that regulates APO1 expression. Thus, we determined the amount of eight phytohormones. As a result, levels of jasmonate (JA), jasmonoylisoleucine (JA-Ile), and abscisic acid (ABA) decreased significantly in Sasanishiki by ozone and these phytohormones were increased significantly in Habataki. These results indicate that ozoneinduced grain yield loss in Habataki

CS02-03 Quantitative trait loci and their interactions regulating the flowering time variation and photoperiodic response in rice P. Subudhi, T. DeLeon, R. Karan, P. Singh, A. Parco School of Plant, Environmental, and Soil Sciences, Louisiana State University Agricultural Center, Baton Rouge, Los Angeles, United States Heading date in rice is a critical attribute that determines both productivity and adaptation to different cropping seasons and geographical areas. The objective of this study was to investigate the genetics of heading date and response to photoperiod. Two recombinant inbred line (RIL) populations and a chromosome segment substitution line (CSSL) population involving two high yielding rice cultivars and one photoinsensitive weedy rice accession were evaluated for heading date under natural field conditions. Sequences and transcript abundance of few genes involved in flowering time regulation were compared among the parents and selected photosensitive CSSLs. Four to seven quantitative trait loci (QTLs) were identified in above two RIL populations and some of these QTLs were validated in CSSLs. Three photosensitive CSSLs were identified and two of them in both

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cultivar backgrounds were having a chromosome 6 segment of weedy rice most likely harboring Hd1. Another CSSL with a chromosome 4 segment of weedy rice was sensitive to photoperiod in only one cultivar background. Day neutral nature of the weedy rice may be due to genetic interaction between Hd1 and a locus on chromosome 7. The transcript abundance of flowering time genes indicated that a new gene on chromosome 4 may be responsible for photoperiod sensitivity in the CSSL and interaction involving this gene might have resulted in loss of photoperiod sensitivity in weedy rice. Understanding the genetic basis of hidden diversity underlying heading date variation under different photoperiodic regime will facilitate exploitation of wild and weedy rice in rice breeding program.

enhancement of source strength such as leaf photosynthesis is expected to be important to increase rice yield potential. Natural variation in light-saturated photosynthesis rate has been widely observed among rice cultivars. However, there has been little progress in genetic analysis for leaf photosynthesis, owing to the complexity of this trait. We devised effective means of phenotyping and mapping QTL involving leaf photosynthesis. We will present a series of challenges toward cloning of photosynthesis QTL. CS02-05 Molecular mapping and introgression of novel yield enhancing QTLs from O. longistaminata B. Marathi, J.M. Ramos, K.K. Jena

CS02-04

Plant Breeding, Genetics, and Biotechnology, International Rice Research Institute (IRRI), Los Baños, Philippines

identification and characterization of QTL involving rate of photosynthesis in high yielding rice

Increasing the yield potential is the major focus of rice breeding program. One of the main constraints for yield improvement is the narrow genetic base in popular cultivars. Recent reports from molecular mapping studies in different crops including rice indicate that phenotypically inferior wild species can contribute favourable genes for yield and thus providing a novel way for exploitation of wild relatives to broaden the genetic base to improve yield. The AA genome African wild species, O. longistaminata is a perennial allogamous species was crossed with IR64 to

T. Takai NARO Institute of Crop Science, Japan Rice yield is determined by the balance of a number of factors, including sink size, source strength, and carbohydrate translocation. Recent progress in rice genomics has promoted cloning genes underlying quantitative trait loci (QTLs) associated with sink size such as grain number and grain size. However, sink size-enhanced-lines do not often perform expected yield due to the decline of grain filling. Therefore,

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National Institute of Crop Science, NARO, Tsukuba, Japan

develop a BC2F2 population for mapping of yield and yield component traits. The mean performance of traits in parents and range of trait values of population indicates transgressive segregation in favorable direction for all traits. Most of the yield component traits were normally distributed and skewed towards cultivated rice. A linkage map was constructed with 158 polymorphic markers and the total length of the map was 1840 cM with average marker interval of 12.6 cM. A total of 30 QTLs were identified for 9 yield and yield contributing traits. Major QTLs for yield traits were validated in Nipponbare/O.longistaminta and Taichung 65/O.longistaminta chromosome segment substitution lines and in IR64/O.longistaminta advanced backcross lines. The major QTLs for yield traits were introgressed into an elite New Plant Type (NPT) variety IR65600-81-5-3-2. The improved NPT lines with introgressed QTL regions from O. longistaminata showed increased panicle size and grain yield compared to the recurrent parent variety.

A huge increase in rice production is essential especially in developing countries of Asia and Africa, where indica-type rice is predominantly consumed. The development of highyielding varieties is a pressing issue to feed an increasing population. The IRRIJapan collaborative research project has been enhancing the yield potential of indica-type varieties (IR64) by introgressing useful genes from 1st generation of New Plant Type varieties as a donor parent. A set of 334 chromosomal segment introgression lines (ILs: BC3-derived lines) with elite agronomic traits were successfully developed in the genetic background of IR64. Based on an association analysis between agronomic traits and the genotype of the 334 ILs, QTLs for total spikelet number per panicles (TSN), grain weight (GW), and days to heading (DTH) were detected by SSR markers. Near-isogenic lines (NILs) with high TSN, heavier GW, or long DTH have been developed so far. The effect of QTLs on a specific agronomic trait was confirmed in the field at IRRI. Some of the promising ILs and NILs are being evaluated under several environments to be linked with the practical product. Molecular marker-assisted breeding is also being implemented to transfer QTLs to other elite indica-type genetic backgrounds. Detected QTLs for the genetic improvement of the yield potential in indica-type varieties will be described.

CS02-06 genetic improvement of the yield potential of indica-type rice by using japonica-type varieties 1, 2 1, 3 1,2 T. Ishimaru , D. Fujita , K. Sasaki , 2 2 2 M. Obara , S. Yanagihara , Y. Fukuta , N. 1,2,3 Kobayashi 1

International Rice Research Institute (IRRI), Los Baños, Philippines 2 Japan International Research Centre for Agricultural Sciences (JIRCAS), Tsukuba, Japan

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CS02-07

found in the loci of DEP1, DEP2, and Er1(t) from O. meridionalis and O. barthii. Genetic study and mapping work on ILs from O. nivara, O. rufipogon and O. sativa are ongoing. More loci/allele responsible for erect panicle identified from different AA species will help to shed light on the genetic mechanism underlying panicle development and lay the foundation for the utilization of genes for erect panicle in rice breeding program.

introgression of erect panicle genes (QTL) from aa genome species to increase yield potential in rice (Oryza sativa) 1

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D. Tao , J. Zhou , Z. Wu , P. Xu , J. Li , 1 1 1 1 Y. Zhang , X. Deng , F. Hu , W. Deng , Y. 2 Yang 1

Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China 2 Life-sciences School, Yunnan University, Kunming, China

CS03-01 Jasmonate-dependent transcriptional regulation in response to different environmental cues

Identifying erect panicle genes from wild rice species will be beneficial to rice breeding programs and enhance genetic diversity of cultivated rice. Among 6588 ILs with different traits in Yundao 1, Dianjingyou 1, and RD23, from 8 AA genome species in Genus Oryza, seventy-two ILs with erect panicle phenotype in Yundao 1 or Dianjingyou 1 background were raised from all AA genome species but O. longistaminata. Genetic analysis and mapping study using populations between ILs and recurrent parent were done. And ER1(t) from O. glaberrima was identified in the long arm of chromosome 4 between SSR marker RM3332 and RM5879. EP4 was mapped in the short arm of chromosome 6 between SSR marker RM7420 and RM19623 from O. glumaepatul. qEP1.1 was found in the long arm of chromosome 1 between RM583 and RM129 from O. meridionalis. qEP8.1 was detected in the short arm of chromosome 8 between RM407 and RM3309 from O. meridionalis. Furthermore, allelic diversities were

M. Riemann, K. Svyatyna, R. Dhakarey, M. Hazman, R. Brendel, P. Nick Karlsruhe Institute of Technology, Karlsruhe, Germany It is crucial for plants to respond to their environment. Plant hormones are important signalling compounds contributing to a fast information flow in cells, tissues and the whole organism. We isolated mutants in different steps of the biosynthesis of the plant hormone jasmonate and currently analyze its function in rice development and stress responses. As examples, data for the involvement of jasmonate in photomorphogenesis, the developmental program that is mediated by phytochrome (phy) and other photoreceptors, and mechanical wounding are presented. The mutants we isolated react differentially to darkness and light. Their growth of

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coleoptiles is promoted by light, while that of wild-type coleoptiles is inhibited by light. Amongst those mutants coleoptile photomorphogenesis 2 (cpm2) and hebiba were shown to be deficient in jasmonic acid (JA) and to lack the function of one of the key enzymes of jasmonate biosynthesis, ALLENE OXIDE CYCLASE (AOC). This gene is present as a single copy gene in rice, making those mutants a brilliant material to study the function of jasmonate. Tos17-insertion lines of the rice orthologue of JASMONATE RESISTANT 1 (JAR1), which encodes an isoleucineJA conjugating enzyme, showed a clear photomorphgenic phenotype, although it was somewhat distinct from that of JA biosynthesis mutants. Comparing the hormonal regulation and expression of representative, JA-dependent genes, we show that OsJAR1 is required for the response to mechanical wounding, while its function can be partially complemented by redundantly active enzymes in photomorphogenesis.

variations in rice lines, engineering of plants with specific expression pattern and for systematic reconstruction of the gene network. The problem of regulatory motif discovery in is a classic bioinformatics challenge and many solutions have been proposed. We proposed a simple and robust method discovering motifs in DNA sequences using gene expression data. To assess the accuracy of the predictions we designed a benchmark which was used to compare our method with other approaches. The method is illustrated with examples of tissue specific and stress-related motifs in rice genome. CS03-03 Tiny rnas big impact on genome regulation A. Jha Studio of Computational Biology and Bioinformatics, Studio of Computational Biology and Bioinformatics, CSIR Institute of Himalyan Bioresource and Technology, Palampur, Himachal Pradesh, India

CS03-02 genome-Wide Discovery of ciselements in Promoter Sequences

Small non coding RNAs such as microRNAs play a significant role in regulating gene expression. For a long time it was believed that miRNAs transcriptional biogenesis was host gene dependent. Where the host gene's transcription machinery are used for miRNA transcription. To understand the complex nature of miRNA biogenesis, our lab performed genome wide analysis for miRNA biogenesis for plant and animal species. The miRNAs precursors

N. Alexandrov International Rice Research Institute (IRRI), Los Baños, Philippines Gene expression is regulated by transcription factors interacting with specific functional sequence motifs in promoters, UTRs and introns. Identifying functional motifs is essential for understanding the effect of genetic

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USDA-ARS Western Regional Research Center, Albany, California, United States

appeared to be enriched in transcription factor binding sites regulatory modules as well as RNA binding proteins binding sites in highly position specific manner. miRNA target identification is still a challenge. Using position specific dinucleotide density variation and experimentally validated encoded interaction patterns for miRNA:Target interaction, a machine learning approach, p-TAREF, was developed. p-TAREF was also used to identify targets in rice which was further supported by expression and degradome data. From this analysis it was found that miR156 was an important component of the rice regulatory system, which mainly targeted genes associated with growth and transcription. While working over the miRNAs, it was found that the miRNA information was mainly available for only those species with sequenced genome. The existing miRNA discovery tool essentially required the availability of genomic sequences. Our lab have developed a tool, miReader, which could directly identify the mature miRNAs from small RNA read data without any dependence upon the genomic sequences. Using the same tool, miRNAs were identified in Miscanthus, a species with no reported genomic sequences as well as miRNAs till the date.

Biotechnology is an effective and important method of improving both quality and agronomic traits in rice. We are developing molecular tools for genetic engineering, with a focus on novel transgene expression control elements (i.e. promoters) for rice. A suite of monocot grass promoters that confer distinct patterns of organ-specific or stress-responsive expression in transgenic plants is being created. Candidate genes with distinct patterns of expression were identified and the corresponding upstream promoter sequences were fused to a reporter gene and transformed into rice, wheat, Brachypodium distachyon and/or Arabidopsis plants. The characterization of root-specific, pollen-specific, endosperm-specific and green tissuespecific promoters will be presented. We are also developing the capability of genetically engineering rice using only rice-derived sequences. Research utilizing this novel strategy to generate ‘intragenic’ rice plants that carry introduced traits comprised of only native sequences will be discussed. These new molecular tools will enable spatially-defined transgene expression and can potentially facilitate the development of novel genetically engineered rice plants.

CS03-04 novel gene expression Tools for rice Biotechnology

CS03-05 role of Microrna-guided Posttranscriptional gene regulation in Plants

R. Thilmony, M. Cook, M.E. Guttman

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R. Sunkar

families. These findings as well as miRNAs that play essential roles in adaptation to stress conditions in Arabidopsis and rice will be discussed.

Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma, United States

CS03-06

Recently discovered genome-encoded small RNAs have emerged as critical regulators of gene expression in higher eukaryotes. These small RNA can be divided into two sub classes, i.e., 21-nt long microRNAs (miRNAs) and 24-nt long siRNAs. MicroRNAs fine-tune target gene expression at the posttranscriptional level either by degrading or inhibiting the protein production of the mRNA target. On the other hand, 24 nt-long small RNAs regulate gene expression at the transcriptional level by guiding DNA methylation at the target loci, thus modifying the epigenome. Small RNAs, particularly miRNAs have emerged as ubiquitous gene regulatory molecules essential both for the plant development and adaptation to the environmental stresses. To gain an insight into miRNA networks that control various traits including stress tolerance in rice, several millions of small RNAs were sequenced. Sequence analyses revealed the identification of conserved and novel miRNA families. The integration of miRNAs in diverse biological networks relies upon the confirmation of their mRNA targets. To identify miRNA targets in rice, we applied the “degradome sequencing” approach, which globally identifies the remnants of small RNAdirected target cleavage by sequencing the 5’ ends of uncapped RNAs. This analysis has resulted in identification of 153 mRNAs targets of 52 miRNA

Update on gramene database and a study of the rice transcriptome under salt stress 1

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S. Naithani , M.K. K Monaco , S. Wei , J. 2 1 1 Stein , P. Dharmawardhana , J. Preece , 1 2 2 V. Amarasinghe , Y. Jiao , S. Kumari , S. 2 2 2 Pasternak , A. Olson , J. Thomason , K. 2 3 4 Youens-Clark , G. Wu , P. D’Eustachio , 5 5 1 D. Bolser , A. Kerhornou , S. Fox , C. 1 5 5 Sullivan , M. Keays , R. Petryszak , H. 5 3 5 Parkinson , L. Stein , P. Kersey , D. 2,6 1 Ware , P. Jaiswal 1

OSU, Botany and Plant Pathology, Corvallis 2 CSHL, Ware Lab, Cold Spring Harbor 3 OICR, Bioinformatics, Toronto, Canada 4 NYU School of Medicine, Department of Biochemistry & Molecular Pharmacology, New York, United States 5 EMBL-EBI, Genome Campus, Hinxton, United Kingdom 6 USDA-ARS, PSNR, Ithaca Gramene (www.gramene.org), a resource for comparative genomics of plants, currently features 27 full and 10 partial plant genomes in the Ensembl Genome Browser and 10 speciesspecific plant pathway databases, as well as an extensive collection of QTLs, genetic maps and genetic diversity datasets. Gramene has a strong focus on the Oryza genus. In addition to the

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two reference genomes of Asian rice (O. sativa subspecies indica and japonica) this resource includes complete reference assemblies for African rice, O. glaberrima, its wild progenitor, O. barthii, and the more distant relatives, O. punctata and O. brachyantha. We also display chromosome 3 short arm assemblies of eight wild species: O. glumaepatula, O. granulata, O. longistaminata, O. meridionalis, O. officinalis, O. rufipogon, and the polyploid O. minuta (comprising assemblies for both the BB and CC subgenomes), plus the outgroup species Leersia perrieri. The Genome Browser allows users to upload and visualize genomic datasets (e.g. genome-wide SNP associations, QTLs, linkage studies, ESTs, Microarrays, RNA-Seq and proteomics datasets) mapped onto the genome. Several sets of genome-wide data can be displayed simultaneously. Plant Pathways Databases in the BioCyc format allow queries for metabolites, genes, enzymes, and pathways, as well as allows uploading, visualization and analysis of genomic expression datasets. Recently we have started developing two new projects on (1) Plant Reactome, that extends analytic and visualization capabilities of the BioCyc based pathway databases, and (2) a plant gene expression database developed in collaboration with the European Bioinformatics Insititute’s Gene Expression Atlas project. Plant Reactome currently features rice pathways and will include more plant species in the future. The Gene Expression Atlas project is designed to provide gene expression data analyses results from publicly available RNA-Seq and microarray based transcriptome


studies for rice and other model plants. The Atlas data will be accessible via the genome browser hosted by us and from the native Atlas user interface, as well as an option to download in standard formats. As an example, functionalities of Gramene Browser, RiceCyc and Atlas will be illustrated using RNA-Seq data from the salt-tolerant and salt-sensitive cultivars of Oryza sativa after exposure to salinity stress that was generated in our laboratory. Gramene is supported by the National Science Foundation (NSF) award IOS-1127112.

CS03-07 improving salinity tolerance by understanding the significance of allelic variants in HKT1;5 1

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S. Negrao , S.T. Arold , I.S. Pires , I.A. 3 3 1 Abreu , M.M. Oliveira , M. Tester 1

Center for Desert Agriculture, KAUST, Thuwal, Saudi Arabia 2 Computational Bioscience Research Center, KAUST, Thuwal, Saudi Arabia 3 Genomics of Plants Stress, ITQB, Oeiras, Portugal Soil salinity is a major constraint to crop production worldwide. At King Abdullah University of Science and Technology (KAUST) in Saudi Arabia, new partial desalinization technologies are being developed. Thus, if crops can be developed that are able to produce good yields when irrigated with partially desalinized water, a whole new sustainable agronomic system can emerge. Cultivated rice (Oryza sativa L.)

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show the different analyses of the OsHKT1;5 proteins, under salt stress and discuss their utility in breeding efforts for salinity tolerance. Understanding the molecular basis for how particular OsHKT1;5 mutations enhance salt tolerance provides a stepping stone for engineering an OsHKT1;5 mutant that confers high salttolerance.

is the most salt sensitive crop among the cereals, especially during early seedling and reproductive stages. Rice diversity has enabled the identification of a large number of Quantitative Trait Loci (QTLs) linked with salinity tolerance. Among these QTLs, one contains the gene, OsHKT1;5, which is a member of the HKT (High-affinity Potassium Transporter) family. OsHKT1;5 is preferentially expressed in root parenchyma cells surrounding xylem vessels and is responsible for retrieving + Na from xylem sap, thus resulting in a + lower Na shoot accumulation [1]. Genotypic differences between the salttolerant ‘Nonabokra’ and the saltsensitive ‘Koishihikari’ revealed four amino acid changes that enhanced the overall activity of OsHKT1;5 in ‘Nonabokra’ [1]. Our previous work [2] described 14 different OsHKT1;5 proteins among a collection of 392 rice accessions. Phenotyping 59 of these accessions for salinity tolerance allowed a correlative assessment of the relevance of these allelic variations to salinity tolerance. To obtain a mechanistic understanding of the relative importance of these amino acid changes in the haplotypes and their differences in salinity tolerance, we constructed 3D molecular models of all the 14 OsHKT1;5 transporters, based on its structural similarity with the recently resolved KtrAB potassium transporter from Bacillus subtilis [3].In addition, based on the phenotypic characterization of the 14 haplotypes, + we explored the differences found in Na shoot accumulation, biomass parameters and other traits to understand the contributions made by factors other than OsHKT1;5. We will

CS04-01 restructuring of root architecture is a Key to engineer Drought Tolerance J. Kim, J.S. Jeong, Y.S. Kim Seed Biotechnology Institute, GreenBio Science and Technology, Seoul National University, Korea Drought conditions are among the most serious challenges to crop production worldwide. Here, we report the results of field evaluations of transgenic rice plants overexpressing OsNAC5, under the control of either the root-specific (RCc3) or constitutive (GOS2) promoters. Field evaluations over three growing seasons revealed that the grain yield of the RCc3:OsNAC5 and GOS2:OsNAC5 plants were increased by 9-23% and 9-26% under normal conditions, respectively. Under drought conditions however, RCc3:OsNAC5 plants showed a significantly higher grain yield of 22-63%, whilst the GOS2:OsNAC5 plants showed a reduced or similar yield to the nontransgenic (NT) controls. Both the RCc3:OsNAC5 and GOS2:OsNAC5 plants were found to have larger roots

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Wuhan, China

due to an enlarged stele and aerenchyma at flowering stage. Cell numbers per cortex layer and stele of developing roots were higher in both transgenic plants than NT controls, contributing to the increase in root diameter. The root diameter was enlarged to a greater extent in the RCc3:OsNAC5, suggesting the importance of this phenotype for enhanced drought tolerance. Microarray experiments identified 25 up-regulated genes by more than 3-fold (P90% seed set consisting over the seasons Jijay (ACC 76357), Zhakha (ACC 8684), IR 61250-3B-7-1-2-95, IR 2344-P1 PB-9-3-2B and NAN-GUANGZHAN. These donors are being used in crossing with earlier reported heat

P0131 generaTiOn Mean anaLySiS OF DrOUgHT TOLeranCe in neW generaTiOnS OF inTerSPeCiFiC riCe genOTyPeS 1

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J. Lamo , T. Pangirayi , J. Derera , P. 3 Okori 1

Cereals (rice and maize) Program, National Agricultural Research Organization, Kampala, Uganda 2 School of Agriculture Earth & Environmental Sciences, University of KwaZulu-Natal, Pietermaritzburg, South Africa 3 School of Agricultural Sciences, Makerere University, Kampala, Uganda Drought is the major production constraint of upland rice. The magnitude and direction of gene action for drought tolerance at the reproductive stage for spikelet fertility was determined in five populations P1, P2, F1, F2 and F3 generated from two interspecific lines, namely drought tolerant CT 16334 (2)CA-2-M (P1) x WAB 450-1-BL1-136-HB (NERICA 8) (P2) susceptible cross using

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generation mean analysis (GMA). The five populations were planted in the field in a Randomized Complete Block Design (RCBD) in two replicates at 2 seeds per hill at spacing of 20cm x 20cm in the field during dry season at Namulonge, Uganda. Standard cultural practices, including hand planting and hand weeding, were followed. Drought stress was imposed by terminating irrigation when about 50% of the populations had attained an interauricular distance between the flag leaf and penultimate leaf of zero, that is the period about 10 days before anthesis. Rainfall during the trial period and spikeletet fertility at a harvest were recorded. The additive, dominant and epistatic effects were determined following Hayman (1958) model. Additive effects were significant. Narrow sense heritability was high indicating that high a proportion of genetic components of variance can be fixed by selecting in segregating generations indicating that selection for improved drought stress could be conducted in the early generations. Non-allelic interactions were not detected and single gene pair was estimated to control spikelet fertility under drought stress. The new generation of interspecific genotypes could be used in the improvement for drought tolerance.

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NRC on Plant Biotechnology, Indian Council of Agricultural Research, Delhi, India introduction: Annexins are multigene, + multifunctional family of Ca2 binding proteins implicated in various physiological and cellular processes in plants. The present study was undertaken to analyse transcript expression profiling of AnnOsI2, an indica rice annexin by end point RT-PCR under a series of stress from drought tolerant cv. N-22 and sensitive cv. IR64. Methodology: The treatments included exposure to CaCl2 at different concentrations in a time course experiment, water stress for 48 hrs at 6 critical developmental stages and biotic stress of 3 different insect infestations in 3 developmental stages. For functional studies AnnOsI2 was cloned in yeast vector pYES2, mobilized to S. cerevisae and expression of recombinant protein analysed under a series of abiotic stress. results: Differential induction of AnnOsI2 was observed under both biotic and abiotic stimuli at different growth stages and time points. Comparatively higher induction of AnnOsI2 was observed in N22 drought stressed seedling over all other stages. At 0.5mM CaCl2, AnnOsI2 was up regulated at 15 min. and gradually decreased over time. Among biotic stress, the best induction of expression was observed in Brown Plant Hopper infected samples in tillering and grain filling stages. Enhanced tolerance of AnnOsI2 was also observed in S. cerevisae

P0132 annOSi2, a MULTiFUnCTiOnaL anneXin gene inVOLVeD in aBiOTiC anD BiOTiC STreSS in riCe S. Deka

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Discussion and conclusion: The involvement of AnnOsI2 under multiple stresses indicates its role in stress abatement. Further functional analysis in engineered plants will show its exact role in stress tolerance.

LBS and RDW showed wide variation among the varieties under iron toxicity treatment. Landrace and upland varieties showed higher scores of LBS than those of improved and lowland ones. Upland varieties showed higher values of RDW than those of lowland ones. There was no relation between LBS and RDW among the varieties(r=0.005). Some upland NERICAs and a chromosome segment substitution line, CSC-194 with Milyang23 chromosome segments in Akihikari genetic background, showed higher values of RDW than those of their parents. These varieties were selected as tolerant materials for detecting genetic factors for tolerance of iron toxicity.

P0133 geneTiC VariaTiOn OF TOLeranCe FOr irOn TOXiCiTy USing agar nUTrienT SOLUTiOn in riCe 1

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A. Tomita , J. Pariasca Tanaka , M. 2 1 Wissuwa , Y. Fukuta 1

Biological Resources Division, JIRCAS, Tsukuba, Japan 2 Crop and Environment Division, JIRCAS, Tsukuba, Japan

P0134 WHOLe genOMe aSSOCiaTiOn MaPPing FOr rOOT MOrPHOLOgiCaL anD anaTOMiCaL TraiTS in a JaPOniCa PaneL UnDer niTrOgen DeFiCienCy

Evaluation for iron toxicity in hydroponic culture is difficult to correspond with those under field condition. Wang et al. (2008) reported agar nutrient solution showed similar results under field condition. To detect genetic factors for tolerance of iron toxicity, we investigated genetic variation of tolerance for iron toxicity using agar nutrient solution. Three-weeks-old rice seedlings of 103 varieties, including Japonica and Indica types, landrace and improved types, and lowland and upland types, were grown in nutrient solution added 0.1 % (w/v) agar under 200 ppm of FeSO4 for 14 days. Leaf bronzing score (LBS, 0-5) and relative dry weight (RDW, ratio of dry matter weight under the treatment/dry matter weight under normal condition×100) were investigated after the treatment.

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E. THOMAS , J. SOEWARTO , A. 1 1 DARDOU , A. AUDEBERT , A. 1 1 DIEVART , C. PERIN , E. 1 1 GUIDERDONI , B. COURTOIS 1

UMR AGAP, CIRAD, Montpellier, France Introduction Improving nitrogen use efficiency (NUE) of rice varieties is of major importance for farmers with limited access to fertilizers. One way to improve NUE is by acting on nitrogen (N) uptake by roots. Root traits such as number, length, and

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ramification are keys to N acquisition. The objective of this study was therefore to understand the genetic mechanisms controlling root architecture under N deficiency. Methodology We conducted an association mapping study on a panel of 167 japonica accessions that had been genotyped with 16,644 SNP markers. We evaluated the root traits of plants grown under favorable (4 mM N) and N-limiting conditions (2.0 and 0.5 mM N) in a semihydroponic system. Measurements were performed on morphological traits (root number, depth and distribution) as well as anatomical traits (root, cortex and stele diameters; number and area of xylem vessels). Results and discussion The treatment effect (favorable versus limiting N conditions) was significant for most traits, with a large genetic variability. Statistical analyses using a mixed model enabled us to detect several markers significantly linked with morphological or anatomical traits. We detected few common markers across treatments, suggesting strong QTL x treatment interactions. Within each individual treatment, co-localizations between markers related to different traits were observed, an indication that some genes have a pleiotropic effect. Few markers co-localized with genes known for their function in root development or nitrogen transport in rice. Additional experiments will help confirm the interest of the significant markers in predicting root phenotypes.

DeVeLOPMenT OF riCe HyBriDS WiTH aBiOTiC STreSS TOLeranCe 1

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U. prasadarao , S. satishreddy , C. 1 1 ramakrishna , P. satishkumar 1

Research and Development, nuziveeduseeds pvt ltd, HYDERABAD, India Hybrid vigour resulting in higher yield coupled with tolerance to abiotic stresses like cold or drought makes hybrid rice popular in larger areas in India and elsewhere Materials and Methods: The cold tolerant variety Kalimpong-1 which was heterogeneous was subjected to secondary selection and the culture Kalimpong -1 SS-19 was selected. The culture was found to give fertility restoration with CMS line IR 58025A and the hybrid NPH 924-1 was developed by using it as a male parent. Similarly, the variety IET 5656 was back crossed with the IRRI restorer line IR 59624 and a drought tolerant restorer line PNPH 24R was developed and by making it the male parent to IR 58025A, the hybrid PNPH 24 was developed. results and Discussion NPH 924-1 showed 25% yield superiority in the multilocation trials of NSL during boro season in suitable areas. NPH 924-1 (IET21255) was tested in AICRIP trials during 2009 – 2011 and based on its yield superiority (23.8% over the best check) it was released by Govt. of India for boro areas in West Bengal and Assam. NPH 924-1 with medium slender grains, dense

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panicle matures in 130 days during boro season.

approaches using bi-parental mapping populations, thereby covering only a narrow genetic basis. Here we report a genome wide association study (GWAS), identifying iron tolerance loci in a panel of 329 varieties, representing all subgroups of O. sativa. The lines were grown in hydroponic solutions in the green house for four weeks and then exposed to an iron pulse stress of 1000 2+ ppm Fe for five days. All plants were phenotyped by quantifying leaf damage and growth. Temperate japonica and aromatic sub-groups proved more tolerant than tropical japonica, indica and aus (p < 0,001). SNP markers significantly associated with iron tolerance were located near or inside potential candidate genes predicted or demonstrated to function in metal transport and detoxification. Currently, a subset of 32 selected lines contrasting in iron tolerance is being validated under field conditions on an iron toxic acid sulfate soil in Iloilo, Philippines. This project will add to the genetic understanding of iron uptake, transport and tolerance mechanisms, which is the basis for enhancing yield and producing fortified crops on iron toxic soils.

PNPH 24 recorded superior yields in NSL multilocation trials. It was subsequently tested in AICRIP trials (2009-2011) and based on its higher yield,(11.1% over check) PNPH 24 (IET 21406) was released by Govt. Of India for growing in the states of Bihar, West Bengal and Odisha.. Efforts are being made to develop hybrids tolerant to problem soils. P0136 a genOMe WiDe aSSOCiaTiOn STUDy (gWaS) TO iDenTiFy geneS aSSOCiaTeD WiTH TOLeranCe TO irOn TOXiCiTy in riCe (Oryza SaTiVa L.) 1

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E. Matthus , L.B. Wu , G.B. Gregorio , 1 1 M. Becker , M. Frei 1

Department of Plant Nutrition of the Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany 2 Plant Breeding Genetics and Biotechnology, International Rice Research Institute, Los Banos, Philippines

P0137 THe riCe OSPSTOL1 PrOTein KinaSe in THe PUP1 MaJOr QTL COnFerS TOLeranCe OF PHOSPHOrUS DeFiCienCy

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Iron (Fe ) toxicity limits rice (Oryza sativa L.) growth in paddy fields worldwide, reducing yields up to 2+ complete crop failure. Fe leads to the formation of reactive oxygen species (ROS) within the plant, followed by typical bronzing of the leaves. Previous work aimed at genetically dissecting this complex trait has focused mainly on QTL

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R. Gamuyao , J.H. Chin , J. Pariasca2 3 1 Tanaka , P. Pesaresi , S. Catausan , C. 1 1 Dalid , I. Slamet-Loedin , E. Tecson4 2 5 Mendoza , M. Wissuwa , S. Heuer

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PBGB Division, International Rice Research Institute, Los Baños, Philippines 2 JIRCAS, Japan International Research Center for Agricultural Sciences, Tsukuba, Japan 3 Dipartimento di Bioscienze, Universita` degli studi di Milano, Milano, Italy 4 Institute of Plant Breeding, University of the Philippines, Los Baños, Philippines 5 ACPFG, Australian Center for Plant Functional Genomics, Adelaide, Australia Phosphorus (P) is an important major element for crop production. However, P is not easily available to the plants due to P-fixation in the soil, and additionally the phosphate rock which is the source of P-fertilizer is a diminishing, nonrenewable resource. Providing opportunities for the development of Pefficient rice varieties is the identification of the major rice quantitative trait locus (QTL) Phosphorus Uptake 1 (Pup1). For more than a decade, the underlying tolerance mechanism remained unknown as earlier studies on Pup1 function were not associated with Puptake related physiological mechanisms or obvious candidate genes. Sequencing of the Pup1 locus in the tolerant donor Kasalath showed the presence of a novel protein kinase gene, named PHOSPHORUS STARVATION TOLERANCE 1 (OsPSTOL1), that is located within the Kasalath specific insertion-deletion region that is missing in Nipponbare. Overexpression of the PSTOL1 gene, which encodes a functional Ser/Thr protein kinase, conferred tolerance of P deficiency to two rice varieties (i.e., IR64, Nipponbare)

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which inherently lack the gene. This gene is expressed in root primordia and acts as an enhancer of root growth providing plants with an advantage in foraging the soil for P and other nutrients. Gene-specific markers have been developed to facilitate introgression of the Pup1 QTL into local rice varieties to improve productivity in areas with low soil P conditions. Future breeding prospects also include pyramiding of this locus with other high-value QTLs (i.e., anaerobic germination, drought tolerance) for the development of rice varieties with broad stress tolerance to support rice production. P0138 Fine MaPPing OF QTL FOr HeaT TOLeranCe OF riCe On CHrOMOSOMe 1 1

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H. Thein , C. Ye , F. Tenorio , Y.O.Y.E. 1 1 Yu , E. Redona 1

International Network for Genetic Evaluation of Rice PBGB, International Rice Research Institute, Los Baños, Philippines

Fine mapping of identified QTL is important to understand the genetic effect of the QTL and to identify potential candidate gene for the trait. We had previously identified two QTLs related to spikelet fertility under high temperature at flowering stage in an IR64/N22 population. To fine map the heat tolerance QTL on chromosome 1 (qHTSF1.1), backcross populations (BC2F1, BC2F2, and BC2F3) were developed and genotyped by using SNP

(single nucleotide polymorphism) markers in the QTL region. Selected plants were treated at 38°C during flowering stage, and spikelet fertility was evaluated. There were 10 BC2F1 plants (out of 24) showed recombination in the QTL region. 342 plants from the 10 BC2F1 were genotyped, and 24 plants from each line were selected for phenotyping. The QTL was confirmed to be located between SNP marker 61 and 63, and the introgression of the N22 genome in the QTL region is within 37629 - 38007 kb, or about 144.7-146.2 cM. Further fine mapping of qHTSF1.1 using advanced backcrossing populations (NILs- near isogenic lines) is ongoing. The SNP markers developed in this study can be used in marker assisted selection (MAS) for introducing the QTL to other breeding lines and varieties. Key words: QTL (Quantitative trait loci), SNP(single nucleotide polymorphism), MAS(marker assisted selection).

rice anthesis, the most sensitive stage to high temperature, may lead to complete spikelet sterility. The degree of spikelet sterility under high temperature is affected by humidity since as relative humidity (RH) increases, spikelet fertility decreases. This study aimed to identify rice varieties with heat tolerance at anthesis under different RH, and to achieve a better understanding of how temperature and humidity affect rice spikelet fertility at flowering stage. Fifteen rice varieties were planted in the net house with natural air temperature and sunlight. Treatment was done in Indoor Growth Chambers (IGC) with same high temperature (38°C) but with different RH (50%, 70%, and 90%) settings. When the plant started heading, it was moved into IGC. After the plant finished flowering, it was moved back to the net house until harvest. The heat tolerance of each variety was based on spikelet fertility. Wide variation in the spikelet fertility was found among the tested rice varieties. Some showed tolerance under low RH while others showed tolerance under high RH at 38°C. The information gathered from this study is very useful for developing future breeding strategy for the hot-dry and hot-wet regions. Keywords: Rice, heat tolerance, anthesis, spikelet fertility, relative humidity

P0139 riCe HeaT TOLeranCe UnDer DiFFerenT reLaTiVe HUMiDiTy DUring anTHeSiS 1

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F. Tenorio , C. Ye , H. Thein , Y. Yu , E. 1 Redoña 1

PBGB, International Rice Research Institute, Los Baños, Philippines

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Any episode of high temperature, which is expected to occur more frequently in the near future, becomes a serious problem in rice production. Further increase in temperature specially during

reSTOring anD enHanCing riCe PrODUCTiViTy OF COaSTaL SaLine areaS OF anDaMan & niCOBar ISLANDS THROUGH FARMERS’ ParTiCiPaTOry VarieTy

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evaluation of salt tolerant rice varieties at farmers’ fields in the islands. During wet season 2011, farmers’ participatory variety selection (PVS) was conducted in South Andaman district. A total of 17 salt tolerant rice varieties were evaluated in three replications under moderately saline (ECe~ 4.00 dS/m) and acid sulphate (pH2~5.5) soils. Among genotypes evaluated, variety CST 7-1 gave highest yield (4.03 t/ha) followed by CARI Dhan 5 (3.91 t/ha). However, the preference analysis by involving 41 men and women farmers in South Andaman revealed maximum preference for variety CARI Dhan-5 followed by CSRC (S)21-25. Similarly PVS evaluation in Middle Andaman during wet season 2012 revealed that though CSR36 yielded the highest (2.85 t/ ha) followed by STBN21 (2.37 t/ha), yet farmers exhibited maximum preference for CARI Dhan 5 and CSR36. In view of preference and demand by farmers, about 20 quintal seed of these salt tolerant rice varieties has been distributed in the areas affected by problem soils in the islands.

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R.K. Gautam , P.K. Singh , S.K. Zamir 2 1 1 Ahmed , A.K. Singh , N. Kumar , A. 3 4 5 Velmurugan , S. Dam Roy , R.K. Singh , 5 6 G.B. Gregorio , A.M. Ismail 1

Division of Field Crops, Central Agricultural Research Institute (CARI), Port Blair, India 2 Social Science Section, Central Agricultural Research Institute (CARI), Port Blair, India 3 Division of Natural Resource Management, Central Agricultural Research Institute (CARI), Port Blair, India 4 Central Agricultural Research Institute, Central Agricultural Research Institute (CARI), Port Blair, India 5 Division of Plant Breeding Genetics and Biotechnology, International Rice Research Institute, Los Banos Manila, Philippines 6 Division of Crop Soil & Water Sciences, International Rice Research Institute, Los Banos Manila, Philippines Rice is the mainstay cereal crop of Andaman and Nicobar Islands (India). The adverse impacts of tsunami in 2004 and vulnerability of the islands to the effects of climate change call for concerted efforts for restoring and enhancing rice productivity for sustaining islands’ livelihood. In the coast line, the crop often encounters dual soil stress of moderate salinity and acid sulphate soils. Therefore, development, evaluation and dissemination of abiotic stress tolerant rice varieties through farmers’ participation are essentially required. The present work carried out under IRRI-ICAR-STRASA project included

P0141 QTLS aSSOCiaTeD WiTH anaerOBiC gerMinaTiOn OF riCe(Oryza SaTiVa) 1

S. Hsu , C. Tung

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1

Agronomy, National Taiwan University, Taipei, Taiwan Direct seeding has become a popular method in some rice growing area in order to reduce the cost for transplanting,

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however, poor crop establishment after direct seeding is the major obstacle to overcome. Observation that coleoptiles of rice seedlings elongate upon hypoxia stress triggers our curiosity to investigate how coleoptile elongation relates to tolerance of anaerobic condition. So far, limited number of QTLs associated with anaerobic germination has been identified. In our study, we measured the length of coleoptile elongation under 5cm of water as a trait. QTL mapping was conducted using Nipponbare X IR64 mapping population consisting of 155 RILs genotyped with a set of 35,501 SNPs marker. By single marker analysis, several SNPs were highly correlated with the phenotypic variation. Four significant QTLs were detected on chromosome 1, 6, 7 and 10, with -log10 (p-value) of 4.071-9.375. Among these QTLs, some participate in the anaerobic sugar metabolism. According to the previous research on hexokinase-mediated sugar signaling to promote rice tolerance to flooding, we suggest that one putative QTL encoding hexokinase on chromosome 1 may act upstream of sugar signaling-pathway when responding to hypoxia. Consequently, the signal transduction may affect the downstream sugar metabolism to trigger tolerance to hypoxia and provide sufficient energy for coleoptile elongation to escape from the stress. Understanding the role of hexokinase in sugar signaling pathway might be the key to reveal the secret of anaerobic germination, further studies are needed.

iDenTiFiCaTiOn OF nOVeL QTLS aSSOCiaTeD WiTH SaLiniTy TOLeranCe in riCe aT SeeDLing STage USing CHiKiraM PaTnai/azUCena CrOSS 1

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M. Sarker , M.A. Newaz , M.P. de 3 4 3 Ocampo , M.J. Thomson , A.M. Ismail 1

Plant Breeding, Bangladesh Rice Research Institute, Gazipur, Bangladesh 2 Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh 3 Crop and Environmental Sciences Division, International Rice Research Institute, Los Banos, Philippines 4 Plnat Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Banos, Philippines Rice is highly sensitive to salt stress at seedling and reproductive stages, and tolerance at seedling stage is particularly important for crop establishment in coastal areas where salinity is high at the beginning of the rainy season. A mapping population of 250 F2 individuals and their corresponding F3 families, derived from a cross between Chikiram Patnai (Indica) and Azucena (Japonica) was used to map QTLs for salt tolerance at seedling stage. Phenotyping was performed in hydroponics with salt stress of EC 12 dS m-1 using F2:3 families and 15 traits associated with salinity tolerance at seedling stage were assessed. A linkage map was constructed with 111 SSR/InDel markers spanning the 12 chromosomes, with total length of

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Philippine Los Banos, Los Banos, Philippines

2081.0 cM, and an average distance of 18.8 cM between markers. A total of 37 QTLs were identified by single marker analysis (SMA), interval mapping (IM) and composite interval mapping (CIM), with an LOD ? 3.0. Five major QTLs were detected for shoot Na+ (qNa1.1) and K+ (qK1.1) concentrations, shoot Na+-K+ ratio (qNaK1.1), root K+ (qRK1.1) concentration and root Na+-K+ ratio (qR-NaK1.1) on the short arm of chromosome 1 with LOD values in the range of 4.2-12.9 and phenotypic variance of 7.5-21.2%. The major QTLs, qSES1.1, qRL1.1, qRDW11.1, qR-Na6.1 and qR-NaK8.1 on chromosomes 1, 11, 6 and 8 are novel in this study. These QTLs could further be exploited for fine mapping, gene discovery and for subsequent use in breeding by pyramiding them with other QTLs for salinity tolerance or with QTLs associated other abiotic stresses.

Identification and introgression of large and consistent effect QTL in the background of elite but drought susceptible varieties is an efficient breeding strategy to improve yield under drought in rice. Our study identified largeand consistent-effect QTL associated to grain yield under drought stress using two mapping populations derived from crossing Kali Aus, a drought tolerant rice landrace from India with two mega varieties: IR64 and MTU1010 as recipient parents. Two populations were phenotyped for yield and yield component traits under reproductive stage drought stress and non-stress conditions during DS2011 and DS2012 at IRRI. In bulk-segregant analysis, six polymorphic markers: RM246, RM450, RM250, RM232, RM518, and RM19 were found significant in Kali Aus/IR64 mapping population while 8 polymorphic markers: RM495, RM572, RM246, RM211, RM250, RM231, RM3, and RM340 were found significant in Kali Aus/MTU1010 mapping population. QTL analysis revealed major effect grain yield QTLs: qDTY2.3 and qDTY2.2 under drought consistently over two seasons in Kali Aus/IR64 and Kali Aus/MTU1010 respectively. The qDTY2.3 explained 9% phenotypic variance with an additive -1 effect of 451 kg ha whereas qDTY2.2 explained 6% phenotypic variance with -1. an additive effect of 121 kg ha The QTLs showed consistent effect in two stress years and combined stress. These two consistent-effect QTLs: qDTY2.2 and qDTY2.3 can be used to improve the grain yield of the mega

P0143 grain yieLD QTL WiTH COnSiSTenT eFFeCT UnDer rePrODUCTiVe DrOUgHT STreSS in riCe 1

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A. Palanog , B.P.M. Swamy , N. 2 2 3 Shamsudin , S. Dixit , J.E. Hernandez , 3 3 T.H. Borromeo , P.C. Sta. Cruz , A. 2 Kumar 1

Research, Philippine Rice Research Institute, Murcia, Philippines 2 Plant Breeding Genetics and Biotechnology, International Rice Research Institute, Los Banos, Philippines 3 College of Agriculture, University of the

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varieties IR64 and MTU1010 over different severity of drought stress through marker-aided backcrossing and provide farmers with improved varieties that effectively combines high yield potential with good yield under drought.

they were screened genotypically through closely linked marker. result A major QTL trait locus Sub1, associated with submergence tolerance, was mapped on chromosome 9. Thus, an attempt was made to identify the Sub-1 gene in 26 landraces using tightly linked Sub1bc2 indel marker. The 12 landraces showed presence of sub-1 gene at 230 bp with survival percentage of 80-100%. The some lines also had more than 80% survival percentage without sub-1 gene. Discussion and Conclusion The Swarna sub1, Dular, FR13A, FR13B, Goda Heenati, Heensulai, Ausboro, S155, sambha sub1, BD2010-late, IR09F-434 and IR09F-436 possess sub1 gene showed high yield per plant while some other landraces Maha Dikwee, Houra Kani, Naldak, HUR38B do not posses sub1 gene but had high survivability percentage perhaps due to presence of some other gene/QTLs. Result suggested that these lines may be used for RIL and other population development and further mapped for target traits.

P0144 PrOMiSing riCe LanDraCeS FOr SUBMergenCe TOLeranCe 1

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P. Singh , A. Singh , S. Eram , R. Khare , 1 2 A.K. Singh , N.K. Singh 1

Genetics and Plant Breeding, Banaras Hindu University, Varanasi, India 2 National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute New Delhi 110 012, Varanasi, India introduction Flood is one of the most important environmental stress worldwide, damaging among the serious problem which affect over 15 mha areas in South and South-East Asia. Flash flood affecting the plant stands seriously depending on duration of submergence stress. Methodology 144 rice landraces were phenotypically screened for submergence tolerance at B.H.U. Varanasi, India. Twenty one days old healthy seedlings of each landraces was transplanted in submergence pond. After 20 day of transplanting, they were submerged for 15 days, and subsequently desubmerged, and after 10 days of de-submergence, the survival percentage was calculated. Out of 144 landraces only 26 were survived and

P0145 eVaLUaTiOn OF HeaT anD COLD TOLeranCe OF riCe VarieTieS aT BOOTing anD FLOWering STageS 1

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Y. Yu , F. Tenorio , S.V. Jagadish , E. 1 1 Redoña , C. Ye 1

PBGB, International Rice Research Institute, Los Baños, Philippines As a result of climate change, extreme temperature events occurred more

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frequently and had significant negative effect on rice production. High and low temperatures cause major problems for many rice growing countries, from the tropical to temperate countries. Booting and flowering stages are the most sensitive stages to low (35°C) temperature in rice. High or low temperature occurs at these stages can reduce spikelet fertility and ultimately yield of rice. To identify genetic resources tolerant to heat and cold at reproductive stage, five Indica (IR72, N22, IR64, NSIC222, NSIC238), three Indica X Japonica (Giza178, Dasan, Milyang23) and four Japonica (Todorokiwase, Chenoncheong, KeunSeom, TR22183) varieties are exposed to 38/24°C (heat stress) and 21/15°C (cold stress) for 14 days at the booting and flowering stages. Based on spikelet fertility after treatment, the most tolerant variety for heat at booting stage is Cheongcheong. N22 showed the highest heat tolerance at flowering stage. Cheongcheong also showed the highest spikelet fertility for cold treatment at booting stage, and TR22183 showed the highest spikelet fertility for cold treatment at flowering stage. These tolerant varieties are important sources for our future breeding programs for extreme temperature tolerance.

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S. Patil , R. Ronanki , D.A.K. Deborah , 1 1 1 S.S. Jena , A. Srividhya , E.A. Siddiq , 1 L.R. Vemireddy 1

Molecular Biology and Biotechnology, Institute of Biotechnology, HYDERABAD, India

introduction: Identification of candidate genes influencing drought tolerance would help to develop drought tolerant rice varieties through marker-assisted breeding. In the present study, an attempt has been made to identify the candidate genes underlying a QTL cluster governing drought tolerant traits employing nexttranscriptomic generation-based approach i.e., RNA-Seq. Methodology: A QTL cluster on chromosome 8 between RM38 and RM331 governing drought related traits viz., Root Dry Weight and Root Shoot Ratio has been identified from a RIL population of INRC10192/IR64 cross. In order to identify the candidate genes underlying the QTL cluster, RNA-Seq using Ion torrent platform (ABI) has been sought from 20day old root tissues of the parental lines. results: RNA-Seq analysis of the roots shows 4 million and 3.1 million high quality reads, in IR64 and INRC10192 respectively and aligned against the Nipponbare reference genome. A total 9795 and 6901 genes are expressed exclusively in IR-64 and INRC-10192 respectively in addition 52 differentially expressed genes were found. Out of which 18 genes were found to be located within the QTL cluster region on chromosome

P0146 iDenTiFiCaTiOn OF CanDiDaTe geneS UnDerLying a QTL CLUSTer gOVerning DrOUgHT TOLeranT TraiTS eMPLOying rna-SeQ aPPrOaCH in riCe

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prone areas. The specific objectives were to: i. determine farmers’ perception on salt problems and their preferences for rice varieties in the salt affected areas; ii. Identify potential sources of genes for salt tolerance from local and introduced rice genotypes; iii. Introgress salt tolerant genes into farmers preferred varieties; iv. Estimate relative efficiency of direct and indirect selection for tolerance to salinity in rice. v. Determine genetic control of salt tolerance in rice To achieve these objectives the following activities were conducted: a participatory rural appraisal, a screening of varieties for salt tolerance, a crossing of candidate parents using diallel mating design, and evaluation of generated populations.

8 in the marker interval of RM38 and RM331. Discussion and Conclusion: RNA-Seq, a next-generation based root transcriptome of parental lines INRC10192 and IR64 revealed 18 differentially expressed genes within a QTL cluster governing drought tolerant traits on chromosome 8. Functional validation of these genes will help to identify of the candidate gene(s). Introgression of the candidate gene(s) of the QTL region into drought susceptible varieties will aid to develop drought tolerant rice varieties. P0147 BreeDing riCe FOr SaLT TOLeranCe in niger O. SOULEYMANE

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Irrigated crops, INRAN (national institute for agronomic research of Niger), Niamey, Niger

LOW-TeMPeraTUre SenSiTiViTy anD POLLen STeriLiTy are CHaraCTerizeD THe anTHer TRANSCRIPTOME’S REPEAT SeQUenCeS

Salinity stress is one of the most widespread soil limitations in rice growing areas. It is the most serious abiotic constraint of rice production in Niger. Up to 20% of irrigated fields are affected by salt problem. The affected area is still increasing because of human activity particularly superfluous irrigation. Rice is relatively salt-sensitive, and the threshold above which a reduction in yield is anticipated ranges from 1.9 to 1 3dSm- . The broad objective of this study is to expend production and productivity by utilizing salt tolerant varieties in salt

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S. Ishiguro , K. Ogasawara , K. Fujino , 2 1 Y. Sato , Y. Kishima 1

Graduate School of Agriculture Hokkaido University, plant breeding, Sapporo, Japan 2 National Agricultural Research Center, Hokkaido Region, Sapporo, Japan This study focused on the instability of genome-wide expression in rice anthers subjected to low-temperature stress.

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Five rice strains that showed different pollen sterilities due to low temperatures at the booting stage were employed for the genome-wide transcriptional analysis of the anthers using a microarray carrying 32,000 probes designed from repeat sequences and 12,000 probes of genes and miRNAs. The results revealed that the instability of genomewide expression in the rice anther transcripts was detected when the rice plants at the booting stage were exposed to low temperatures. Interestingly, the degree of instability in the genome-wide expression was different among the five strains and coordinated with the degree of the pollen sterility. The instability of genome-wide expression may provide new indicators of the plant’s responses against a given stress. The genome-wide analysis using repeat sequences suggested that stress tolerance could be conferred by insensitivity to the stimuli. This work was supported by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).

QUanTiTaTiVe TraiT LOCi anaLySeS OF rOOT PLaSTiC DeVeLOPMenTaL reSPOnSeS TO PrOgreSSiVe DrOUgHT STreSS in riCe 1

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Philippines Genetic Resources Division, Philippine Rice Research Institute, Science City of Muñoz, Philippines 3 Research Division, Philippine Rice Research Institute, Midsayap, Philippines

and number of L-type LR does not overlap in any of the chromosomal regions indicating that genetic control for plasticity expressions of these two traits in response to progressive drought is independent from each other.

Plastic root system development in rice plays key role in maintaining plant productivity under drought-prone environments such as rainfed lowland ecosystems. This study identified the QTLs associated with droughtavoidance plastic root traits using CT9993/IR62266 doubled haploid lines (DHLs). Eighty-eight DHLs and their parents were grown under continuously waterlogged (CWL) and transient waterlogged to progressive drought (DR) stress conditions for 45 days. Plasticity was estimated as the difference between mean values of the trait in DR and CWL for each DHL. Among the root traits, L-type lateral roots (LR) particularly at 20-40 cm soil depth strongly influenced the plasticity in root system development based on total root length. QTLs identified associated with plasticity in L-type LR length and number at different soil depths contributed 10.3 to 19.5% of the total phenotypic variation. QTL qTLRL30-10 on chromosome 10 was associated with L-type LR length at 20-30 cm soil depth while qTLRL40-10.1, qTLRL40-10.2 and qTLRL40-11 were associated with the trait at soil depth 30-40 cm. On the other hand, qLLRn20-3 and qLLRn30-2, conferring plasticity in L-type LR number at 10-20 cm and 20-30 cm soil depths, were detected on chromosomes 3 and 2, respectively. The results showed that QTLs associated with plasticity in length

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R.R. Suralta , N.B. Lucob , L.M. Perez , 3 J.M. Niones 1

Agonomy Soils and Plant Physiology Division, Philippine Rice Research Institute, Science City of Muñoz,

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varieties is determined, first of all, by complex of unspecific genes. High growth rate and content of photosynthetic pigments in Russian varieties allows them not to store critical salt content and to reduce time of salt impact during stages when rice is most sensitive to the stress.We have studied polymorphism of Russian rice varieties on markers connected with locuses determining growth rate, tolerance to salinity, duration of leaf activity, effectiveness of nitrogen nutrition. Most polymorphic markers for Russian varieties are: RM 259, RM 245, RM 82, RM 284, RM 154, RM 286. Wide polymorphism on locuses connected with studied characteristics allows us to hope on possibility of effective selection.

POLyMOrPHiSM OF rUSSian riCe VarieTieS On LOCUSeS DeTerMining aDaPTaBiLiTy TO STreSS. 1

E. Kharitonov , J. Goncharova

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1

Genetics, All-Russian Rice Research Institute, Krasnodar, Russia Both yield level and its stability in years with different weather conditions depend on level of ecological adaptability. The combination of many abiotic stresses reduces productivity of varieties and hybrids. Mechanisms of tolerance formation are similar for various stresses. Among the characteristics determining them are: rate of cell growth, photosynthetic efficiency, rate of plastic substances attrogation from vegetative organs to generative ones, membranes characteristic, etc. Looking forward we need to study genetic polymorphism of each characteristic determining stability and adaptability of samples to evaluate prospects of their combinatorics. Tasks of our research were to study Russian released and perspective varieties, to search genetic mechanisms of their tolerance for using them in breeding work. In previous researches we have shown that high tolerance to abiotic stresses among Russian

P0151 QTLS MaPPing OF PHySiOLOgiCaL TraiT reLaTeD TO SaLT TOLeranCe in riCe 1

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C. Lee , C.W. Tung 1

Agronomy, National Taiwan University, Taipei city, Taiwan Salinity stress is one of the major abiotic stresses that limited the growth of crops. Since rice is the most salt sensitive among cereals, we hope to identify QTLs that significantly contribute to salt tolerance in rice. In this study we use a diversity panel of rice collected all over the world. By measuring stomata density, plant height, leaf age, leaf area and salt injury score, we expect that accessions with lower injury score (salt tolerant) will have lower stomata density, and smaller

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leaf area would help to reduce the transpiration rate, therefore decrease sodium uptake into leaves or maintain water content in shoot. We measured stomata density and leaf area of the leaf that emerged under stress condition to find out whether stomata density and leaf area would change under salt stress environment. Our result indicates that stomata density has moderate positive relationship with salt injury score, in some accessions, stomata density were increased under salt stress due to the shrinkage of the leaf area. With this result we hope to identify new QTLs that related to salt tolerance in rice.

days, into a 38°C greenhouse. Spikelet sterility was quantified in the top half only of panicles whose overall anthesis was completed by the end of the 6-day period. Several secondary plant traits potentially affecting panicle microclimate and thus the fertilization process, as well as grain filling, were also analyzed. The panel was genotyped with an average density of one marker per 22.9 kb, using genotyping by sequencing. Linkage disequilibrium was 2 high (average r > 0.5 at 25 kb distance). Association analyses were conducted using a mixed model involving both population structure and kinship. Eighteen significant associations (p8.0 t/ha was obtained from varieties MNR1, MNR2, MNR4, MNR5 and OM8108 compared to AS996 with yield of 6.0 t/ha. These varieties were also favoured because of high yield, good grain quality, and tolerance to pest and diseases. In addition, MNR1, MNR2, MNR4, MNR5 were released as varieties in 2012 for commercial production. Seed production and dissemination of these four varieties to other villages were recommended to the Department of Agriculture and Rural Development in Hau Giang province.

P0227 aDaPTaTiOn anD DiSSeMinaTiOn OF aCiD SULFaTe SOiL TOLeranT riCe in MeKOng riVer DeLTa, VieTnaM 1

1

2

N. Thi Lang , B. Phuoc Tam , R. Labios , 1 1 3 N. Van Hieu , P.T. Thu Ha , B. Chi Buu , 4 5 5 R. Reinke , A. Ismail , R. Wassmann 1

Rice Genetics Division, Cuu Long Delta Rice Research Institute, Cantho, Vietnam 2 Agricultural Systems Cluster College of Agriculture, University of the Philippines Los Banos, Los Baños, Philippines 3 Office of the Director General, Institute of Agricultural Sciences for Southern Vietnam, Ho Chi Minh, Vietnam 4 Plant Breeding Genetics and

P0228 insertional mutagenesis to isolate drought and salinity stress tolerant genes in rice 1

1

1

S. Nugroho , U. Musofa , A. Zannati , D. 1 1 Nurdiani , C.F. Pantouw ,

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POSTerS – nOVeMBer 7, 2013

1

Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Indonesia

The groups A and B were mainly, O. sativa varieties, NERICAs, and monogenic lines, while the group C was O. glaberrima varieties. O. barthii varieties were distributed in the three groups. The characterization of West African rice varieties against blast isolates revealed that, these rice were classified into 4 groups a, b, c, and d. West African germplasm is classified into groupsÕ b, c, and d that showed resistant, moderate resistant and strong resistant reactions against blast isolates, respectively. Almost all O. glaberrima varieties were classified in group b and c, while the group-d was O. sativa varieties and NERICAs.

P0001 BLaST reSiSTanCe in aSian riCe (Oryza SaTiVa), aFriCan riCe (O. gLaBerriMa) anD WiLD riCe (O. BarTHii) OF WeST aFriCaT

Over 5,000 single insertion mutant rice lines have been generated at RCBt-LIPI in collaboration with CSIRO, Australia and Wageningen University, the Netherlands. The insertion lines was generated on Japonica rice cv Nipponbare background using two sets of plasmids; activation tagging and gene-trap constructs. Single insertion lines were selected over multiple insertions to facilitate gene identification. The mutant library has been screened for drought and salinity under 200g/L PEG6000 and 200mM NaCl, respectively. Potential drought tolerant mutant lines capable of germinating under PEG have been obtained and the putative responsible genes have been identified. Similarly, salt tolerant and sensitive mutants have been identified and some putative insertion sites responsible for the salt tolerant mutation have been identified. Gene isolations and functional analyses are underway. Keywords: insertional drought, salinity


1

2

3

T. Teophile Odjo , D. Silue , Y. Koide , S. 3 4 5 Yanagihaya , M. Sie , T. Kumashiro , Y. 6 Fukuta 1

Faculty of Agricultural Sciences, University of Abomey-Calavi, Cotonou, Benin 2 Plant Pathology, Africa Rice Center, Cotonou, Benin 3 Biological Resource and Psot-harvest Division, JIRCAS, Tsukuba, Japan 4 Plant Breeding, Africa Rice Center, Cotonou, Benin 5 Program1, Africa Rice Center, Cotonou, Benin 6 Biological Resources and Post-harvest Division, JIRCAS, Tsukuba, Japan

P0002 DiVerSiTy OF riCe gerMPLaSM FOr reSiSTanCe anD PaTHOgeniCiTy OF BLaST FUngUS in JaPan 1

A. Tanaka-Kawasaki , Y. Fukuta 1

United Graduate School of Agricultural SAciences, Tottori University, Tottori, Japan 2 Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Japan

To investigate the genetic diversity and relationship among rice accessions from West Africa, the genotyping by SSR markers and phenotyping by blast isolates were carried out. For the classification and diversity study of resistance, 32 standard differential blast isolates from Japan and West Africa, and total of 196 rice accessions including differential varieties and control varieties were used. And a total of 67 SSR markers were used to classify the genetic diversity of rice in West Africa. Cluster analysis was carried out, and these rice accessions were classified into three major groups, A, B, and C.

mutagenesis,

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2

A total of 324 rice (Oryza sativa L.) accessions including landrace, improved and weed types which adapted to the ecosystems, irrigated lowland and upland in eight regions of Japan, were collected and investigated the genetic variation of resistance to 16 standard differential blast (Magnaporthe oryzae B.

327


Couch) isolates from Japan and the Philippines and these genomic components by the polymorphism data of 64 SSR makers distributing in 12 rice chromosomes. And 310 blast isolates from Japan showed a wide variation in virulence to 25 differential varieties harboring 23 kinds of resistance genes and a susceptible control, Lijiangxintuanheigu. Using the three kinds of data for variations of bleat resistance, genomic chromosomal components of rice and blast races, these relationships between geographical distributions of rice varieties and blast races, and chromosomal components and resistance genes in rice varieties in Japan will be demonstrate and discussed with the basis of gene-for gene theory.

years of cultivation. Because of the variation of blast fungus population overtime that eventually lead to breakdown of resistance. One of the effective and practical approaches for durable protection is maybe the use of multiline variety. Two Indica-type varieties, IR49830-7-12-2, an elite rainfed lowland rice, and IR64, a popular irrigated lowland rice, were used as the genetic backgrounds for the development of multiline varieties. To improve the resistance of IR49830-71-2-2, nine near-isogenic lines (NILs) targeting eight resistance genes *Pik, Pi7, Pi3, Pi5(t), Pita-2, Piz5, Pish and Pi9* were developed. The eleven resistance genes *Pish, Piz, Piz5, Pi9, Pii, Pi3, Pik, Pik-h, Pik-p, Pi1, and Pi7(t)* were introduced in the IR64 genetic background and 22 NILs were developed by recurrent backcross breeding. The reaction patterns of developed NILs were investigated using the standard differential blast isolates, and the introgression of the resistance genes from donor varieties were detected by SSR markers. NILs with morphological traits most similar to those of the recurrent parents were selected. These two multiline varieties with Indica-type genetic backgrounds were the first materials for developing durable protection system against rice blast disease in tropical region.

P0003 DeVeLOPMenT OF MULTiLine VarieTieS FOr BLaST reSiSTanCe geneS WiTH inDiCa-TyPe geneTiC BaCKgrOUnDS 1

M. Mary Jeanie Telebanco-Yanoria , Y. 1 2 Yohei Koide , N. Nobuya Kobayashi , Y. 1 Fukuta 1

Biological Resources and Post-harvest Division, JIRCAS, Tsukuba, Japan 2 Rice Research Division, NICS, Tsukuba, Japan

P0004

Rice blast caused by Pyricularia grisea (Cooke) Sacc.is one of the major constraints in rice growing regions worldwide. Resistant variety is effective and economical but only useful after few

eFFeCT OF TeMPeraTUre On reSiSTanCe gene-MeDiaTeD reSPOnSe TO BaCTeriaL BLigHT OF riCe

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1

1

2

J.F. Balidion , I.R. Choi , R.P. Mauleon , 3 4 1 P.H. Goodwin , K. Satoh , I.P.D. Navea , 4 5 6 S. Kikuchi , K. Wydra , J.E. Leach , C.M. 1 Vera Cruz 1

Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Baños, Philippines 2 Crop Research Informatics Laboratory, International Rice Research Institute, Los Baños, Philippines 3 Department of Environmental Biology, University of Guelph, Guelph, Canada 4 Plant Genome Research Unit Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Japan 5 Fachhochschule Erfurt, University of Applied Sciences, Erfurt, Germany 6 Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins CO, USA Variation in disease reaction relative to growing temperatures has been observed in rice. Biological characterization of bacterial blight (BB) resistance (R) genes Xa4 and Xa7 were done to understand how resistance expression patterns are affected by variation in temperature regimes. Disease severity increased at 35/31¡C (day/night) in a near-isogenic line (NIL) with Xa4 (IRBB4) and IR24 but decreased in a NIL with Xa7 (IRBB7) while decreased severity was observed in IR24, IRBB4 and IRBB7 at 29/21¡C. Temperature affected the spread of Xanthomonas oryzae pv. oryzae (Xoo) in resistant lines, but not its multiplication. Time course transcriptome changes in Xoo-infected

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resistant and susceptible plants showed induction of genes involved in defense and stress responses but genes involved in photosynthesis were generally repressed. Changes in expression of receptor kinases for calcium signalling in the cell wall such as LOC_Os07g03920 and LOC_Os03g12470, which were exclusively up-regulated by bacterial infection in IRBB4 and to some extent in IRBB7 at different temperature regimes, suggest that host defense responses are affected by changes in temperature. Meanwhile, in IRBB67 (Xa4+Xa7), the effect of temperature on disease development lessened relative to that of individual R gene. These results suggest that efficiency of major R genes to BB is affected by temperature. With the warming climate, Xa7 may confer more advantage in terms of disease resistance than Xa4 in both temperature regimes. Resistance gene combinations such as in the case of IRBB67 (Xa4+Xa7) may offer a potential stabilizing effect on disease resistance in plants under thermal stress. P0005 STUDy OF FerTiLiTy reSTOraTiOn OF neWLy DeVeLOPeD CMS LineS OF riCe (Oryza SaTiVa L.) anD aSSeSSMenT OF THeir reSiSTanCe TOBrOWn PLanTHOPPer (niLaParVaTa LUgenS STaL.) 1

2

G. Prahalada , N. Shivakumar , B.P. 3 4 Mallikarjuna , D.K. Sidde Gowda , P. 4 Yadav


were found to be highly susceptible for BPH as they scored 9. Hence, MSN-98 and CRAC 4687-52-1-11-82 utilized in developing BPH resistance hybrids being effective restorers and BPH resistant restorers.

1

Genetics and Plant Breeding, University of Agricultural Sciences, Bangalore, India 2 Genetics and Plant Breeding, Zonal Agriculture Research Station V.C.Farm, Mandya, India 3 Genetics and Plant Breeding, University of Agricultural Sciences, Raichur, India 4 Entomology, Zonal Agriculture Research Station V.C.Farm, Mandya, India

P0006 iDenTiFiCaTiOn OF reSiSTanT genOTyPeS TO BaCTeriaL LeaF BLigHT OF riCe (Xanthomonas oryzae pv.oryzae)

The pollen fertility (%) and spikelet fertility (%) traits used to identify maintainers and restorers which is the prime importance of three line hybrid rice breeding programme from elite breeding lines through test crossing programme. Restorers with resistance to biotic stresses like Brown Planthopper (BPH) resistance will still enhance their utilization in Hybrid rice breeding program as BPH is the most severe damage causing pest of rice. The pollen fertility (%) and spikelet fertility (%) of the newly developed rice hybrids were studied, most of the hybrids expressed potential fertility restoration and eighteen hybrids exhibited complete sterility. Among the genotypes tested, MSN-36, MSN-18-17, MSN-98 and CRAC 468752-1-11-82 recognized as effective restorers for all the six cytoplasmic male sterile lines. The effective restorers were assessed for their resistance pattern for BPH under glass house condition. MSN98 and CRAC 4687-52-1-11-82 scored 5 and 3 of 0-9 scale of BPH damage (IRRI SES, 1996) these results depicted that MSN-98 and CRAC 4687-52-1-11-82 are moderately resistant and resistant for BPH infestation respectively. Six CMS lines used in this investigation

R. COUMARASAMY

1

1

DEPARTMEN OF PLANT PATHOLOGY, PANDIT JAWAHARLAL NEHRU COLLEGE OF AGRICULTURE & RESEARCH INSTITUTE, PONDICHERRY, India Identification of Resistant Genotypes to Bacterial Leaf Blight of Rice ( Xanthomonas oryzae pv. oryzae)

C. Rettinassababady*, R. Renuka and C. Jeyalakshmi P. J. N. College of Agriculture & Research Institute, KARAIKAL, U. T. of Puducherry, India *[email protected] Rice (Oryza sativa L.) is the second important cereal crop after wheat in terms of area, production and consumption in the world. Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is a serious disease of the crop causing yield loss of 40-50 per cent in India depending on location, seasonal weather conditions and cultivars.. Therefore, the long term solution is to deploy resistant cultivars

330


which offers an economically and environmentally safe option of managing BLB of Rice. With this backdrop, a total of 164 rice entries were screened against BLB by adopting clip inoculation method under field conditions. Severity of the disease in each entry was graded on 7days after inoculation and Per cent disease Index (PDI) was worked out and the entries were grouped into Immune, Resistant, Moderately resistant and susceptible based on IRRIÕs Standard Evaluation System. The results revealed that twelve entries were found to be immune to BLB infection recording less than 1Grade. About one hundred and twenty four entries showed resistant reaction and the remaining twenty eight entries recorded moderately resistant reaction to BLB infection. Hence, studies on the identification of ÔRÕ genes in the immune genotypes using novel molecular techniques will pave way for developing resistant varieties with good agronomic traits suitable for cultivation.

at the Rice Experiment Station is to incorporate stem rot resistance into California rice varieties. A wild species of rice, Oryza rufipogon, with a high level of resistance was used to develop a California long grain germplasm designated as 87-Y-550. To understand the genetic basis of stem rot resistance, 87-Y-550 was crossed to a medium grain variety, M-206, to develop a population for genetic mapping. The advanced backcross recombinant inbred lines from 87-Y-550/M-206*2 were generated and genotyped using SSR markers. Replicated disease trials were done for two years and in two locations. QTL analyses identified stem rot resistance QTLs in Chromosomes 3, 6, and 10 consistently observed across years and locations. We are in the process of fine mapping the location of these stem rot resistance genes. The identification and development of markers linked to stem rot resistance would be a useful tool for breeding for improved rice varieties.

P0007 MaPPing STeM rOT reSiSTanCe USing an aDVanCeD BaCKCrOSS POPULaTiOn 1

1

P0008 FUnCTiOnaL MOLeCULar MarKerS FOr BaCTeriaL BLigHT reSiSTanCe gene Xa3 in riCe

1

C. Andaya , J. Oster , G. Yeltatzie , V. 1 Andaya

1

1

1

1

J. Lee , Y.J. Hur , J.H. Cho , H.S. Park , 1 1 1 J.U. Jeung , J.Y. Lee , Y.B. Sohn , Y.C. 1 1 1 Song , D.S. Park , M.H. Nam

1

Rice Experiment Station, California Coop. Rice Res. Foundation Inc., Biggs, USA

1

Department of Functional Crop, Natioal Institute of Crop Science, Mirynag, Korea

Stem rot is a very destructive disease in California causing as much as 25% yield reduction in susceptible varieties. Hence, one of the important breeding objectives

331


Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. Oryzae (Xoo) is a destructive disease in rice worldwide. Xa3, which is a gene conferring resistance to BB at the booting stage of the rice plant, has been characterized previously using mapbased cloning. We cloned and sequenced the Xa3/xa3 gene in the Korean cultivars Hwayeong, Ilmi, and Goun and conferred resistance or susceptibility to BB. We detected polymorphisms, and polymerase chain reaction-based functional markers were developed based on the single nucleotide polymorphism from the Xa3 and xa3 nucleotide sequence. Susceptible or resistant individuals from an F2 population developed from a cross between Milyang 244 and Ilmi, near isogenic lines carrying BB resistance genes, were screened with functional markers. The BB3-RF and -RR primers consistently amplified a resistant-specific fragment of 255bp in only resistant plants, whereas the BB3-SF and -SR primers were specific to susceptible plants. Genotyping results were cosegregated with phenotype by conducting the BB resistance test with the K3 race. These markers could be effective for marker-assisted selection of the Xa3 gene in rice breeding programs.


D. BALAKRISHNAN , S. ROBIN , R. 3 4 RABINDRAN , S. SENTHIL , M. 5 6 Raveendran , A.J. JOEL 1

Plant Breeding and Genetics, Directorate of Rice Research-ICAR, HYDERABAD, India 2 Department of Rice, TAMIL NADU AGRICULTURAL UNIVERSITY, Coimbatore, India 3 Centre for plant protection studies, TAMIL NADU AGRICULTURAL UNIVERSITY, Coimbatore, India 4 Department of Plant Molecular Biology & Bioinformatics (DPMB&B), TAMIL NADU AGRICULTURAL UNIVERSITY, Coimbatore, India 5 Department of Plant Biotechnology, TAMIL NADU AGRICULTURAL UNIVERSITY, Coimbatore, India 6 Department of plant genetic resources, TAMIL NADU AGRICULTURAL UNIVERSITY, Coimbatore, India INTRODUCTION Blast is considered as a major limiting factor in the global rice production because of its wide distribution and destructiveness under favourable conditions. The most effective way to control the disease is the use of resistant varieties, which is more economical and environment friendly than chemical control measures. The gene pyramiding improves resistance spectrum and prevent race evolution. METHODOLOGY The main objectives of this project were to screen the Pi genes introgressed CO 39 NILs for blast resistance and to develop a back cross population using blast resistance genes pyramided CO 39 NIL (CT13432-3R) as the donor parent and a high yielding susceptible variety

P0009 iMPrOVeMenT OF POPULar riCe VarieTy aDT 43 By MarKer aSSiSTeD BaCK CrOSS BreeDing By inTrOgreSSiOn OF BLaST reSiSTanCe geneS

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(IASRI), New Delhi, India Department of Rice, TAMIL NADU AGRICULTURAL UNIVERSITY, Coimbatore, India 4 Centre for plant protection studies, TAMIL NADU AGRICULTURAL UNIVERSITY, Coimbatore, India 5 Department of plant genetic resources, TAMIL NADU AGRICULTURAL UNIVERSITY, Coimbatore, India INTRODUCTION

ADT43 as the recurrent parent. Markerassisted backcross breeding was employed to pyramid three blast resistance genes Pi1, Pi2 and Pi33. RESULTS The advanced generations of F1s, the cross between ADT 43 and CT13432-3R was developed and screened for blast resistance both phenotypically under blast nursery and genotypically using SSR markers linked to blast resistance. The pyramided lines showed a higher level of resistance at epiphytotic conditions. Back ground analysis was carried out in these lines using genome wide SSR markers and obtained a genome recovery upto 94.90 percent. DISCUSSIONS AND CONCLUSIONS The BILs with three gene combinations were found to be highly resistant to blast disease. The selected gene pyramided back cross lines, which were screened phenotypically and genotypically for the blast resistance in a desirable back ground can be advanced to further generations to obtain superior resistant high yielding varieties.

3

Blast disease is one of the serious biotic stresses faced by rice production. Several blast resistant genes have been identified and mapped worldwide. The appropriate knowledge on gene action in a population is essential to understand the genetic architecture of the various traits and accurate selection of breeding methodology. METHODOLOGY In the present study, the promising cross between a susceptible high yielding variety ADT 43 and a blast resistant NIL CT13432-3R was selected. Six generations of this cross including P1, P2, F1, F2, B1 and B2 were used to study the nature and magnitude of gene action for blast resistance, yield and other attributes. The values of individual scaling tests and estimates of gene effects for different traits were estimated. RESULTS The epistatic non allelic interaction model was found adequate to explain the gene action in most of the traits. The interaction was complementary for panicle length, 100 grain weight, lesion number and infested leaf area, but duplicate epistasis was observed for the remaining traits. Variation due to dominant genetic effect was predominant for traits viz., panicle length,

P0010 generaTiOn Mean anaLySiS OF yieLD anD BLaST reSiSTanCe reLaTeD TraiTS in riCe (Oryza SaTiVa L.) 1

2

D. BALAKRISHNAN , A. BISWAS , S. 3 4 5 ROBIN , R. RABINDRAN , A.J. JOEL 1

Plant Breeding and Genetics, Directorate of Rice Research-ICAR, HYDERABAD, India 2 Division of Sample survey, DIndian Agricultural Statistics Research Institute

333


100 grain weight, spikelet fertility, harvest index and single plant yield but additive genetic effect was greater for other traits under study. DISCUSSIONS AND CONCLUSIONS Among the genotypes tested under epiphytotic condition at different environments, lines with three or two gene combinations were found to be highly resistant to blast disease than individuals with single genes indicating that the non-allelic genes have a complementary effect when present together.

years. Then, 30 SSR markers linked with 39 blast resistance genes were used to verify the presence of different blast resistant genes. IRRI developed monogenic lines (Lijiangxintuanheigu and CO39 background) were also used to identify the resistance genes. Rice monogenic lines IR85426 (Pi-ta), IRBLsh-S (Pi-sh), IRBLta-K1 (Pita (= Pi 4(t)), IRBLta-K1 (Pita (= Pi 4(t)), IRBLz5CA (Piz5 (= Pi 2(t)), IRBLzt-T (Piz-t) and IR85427 (Piz-5) showed good resistance against leaf blast and neck blast. Blast resistant genes Pi-54, Pi-y2(t), Pi-d(t)1, Pi-z, Pi-a, Pi-k, Pi-y1(t) and Pi-44 were detected based on molecular markers on seven, seven, nine, 11, 20 and 28 rice accessions respectively. Eighteen rice accessions showed presence Pi-b and Pi-g(t), 22 rice accessions showed presence of Pi-29 and Pi-11; and two rice accessions showed presence of Pita and Pi20(t). Nepalese rice gene pool doesnÕt contain resistant gene Pi-d(t)2 and need to incorporate from outside. Therefore, many Nepalese rice landraces can be used on gene pyramiding program for development of durable blast resistance varieties.

P0011 eVaLUaTiOn OF nePaLeSe riCe gerMPLaSM FOr BLaST reSiSTanCe CHaraCTeriSTiCS 1

1

R. Amgai , B. Sah , M. Bhatta


2

1

Biotechnology, Nepal Agricultural Research Council, Lalitpur, Nepal 2 National Agricultural Genetic Resource Centre, Nepal Agricultural Research Council, Lalitpur, Nepal Blast is the major yield limiting biotic stress for rice farming in Nepal that is prevalent all over the country. Resistant variety for one location becomes susceptible to another showing the need of resistant gene pyramiding for yield stability. Identifying blast resistant genes in Nepalese rice germplasm and its use for varietal development is very important. One hundred seventy three Nepalese rice accessions were screened in two locations (Lalitpur and Kaski districts) of Nepal for their reaction with natural pathogen population for two

P0012 TraDiTiOnaL riCe LanD raCeS POTenTiaL reSiSTanCe SOUrCeS FOr BreeDing VarieTieS TO BrOWn PLanTHOPPer, niLaParVaTa LUgenS (STaL.) 1

2

D.K. Sidde Gowda , G.M. Dharshini , N. 3 4 Shivakumar , P. Mahadevu 1

AICRP on Rice, University of Agricultural Sciences Bangalore, ZARS

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VC Farm Mandya, India 2 Dept of Entomology, University of Agricultural Sciences Bangalore, Bangalore, India 3 Hybrid Rice, University of Agricultural Sciences Bangalore, ZARS VC Farm Mandya, India 4 Rice breeding, University of Agricultural Sciences Bangalore, ZARS VC Farm Mandya, India

Ratnachoodi 2, Raibhog, Rajamudi, Selamsanna and Moradde could be utilized in resistan c breeding programme against brown planthopper in rice. P0013 MarKer-aSSiSTeD inTrOgreSSiOn OF BaCTeriaL BLigHT anD BLaST reSiSTanCe geneS inTO eLiTe inDian BaSMaTi VarieTieS

Insect pests are the major biotic constraints in rice production. Among the insect pests, Brown planthopper (BPH) Nilaparvata lugens (Stal.)is the most destructive pest of rice in India causing severe yield loss. The use of resistant cultivars has proven to be one of the most efficient ways to reduce the economic damage caused by BPH. Traditional land races are known to be important reservoirs of desirable resistance genes for various biotic and abiotic stresses. In the present study two hundred and eighty landraces collected from different parts of Karnataka were screened in the glass house for their reaction to BPH populations of Mandya, Karnataka, India during 2011-12. Out of 280 landraces, 22 landraces showed varied levels of resistance(damage score of 1-3) to BPH.All the landraces recorded more number of days to wilt, less number of eggs laid, higher per cent of unhatched eggs, low nymphal survival and more number of honeydew secreted spots as compared to the susceptible check TN1.The present study indicated that, not all the traditional landraces are resistant to BPH but, landraces viz., Anilamanil, Baiganmanji, Honasu 1, JBT 36/14, Kalakolli, Kottayam, Manila, Nazarbatta, PS 339, Ratnachoodi 1,

1

1

G. Varaprasad , S. Srikanth , M. 1 1 Pandey , M. Vijay Kumar , N. Shobha 1 1 1 Rani , R.M. Sundaram , G.S. Laha , M. 1 1 Sheshu Madhav , M. Srinivas Prasad , 1 B.C. Viraktamath 1

Crop Improvement, Directorate of Rice Research-ICAR, HYDERABAD, India

introduction: Bacterial blight (BB) and blast are two important biotic stresses which limit yields of Basmati rice significantly. In the present study, we introgressed multiple BB and blast resistance genes into the genetic background of elite Indian Basmati varieties using molecular markers. Methodology: Taroari Basmati and Basmati 386 * two traditional basmati varieties and Vasumati, an evolved variety were used as recurrent parents (RPs) while Improved Samba Mahsuri and Samba Mahsuri possessing Xa21,xa13 and xa5 genes for BB and Pi2 and Pi54 for blast were used as donor parents. Marker assisted backcross breeding was deployed for introgressing target genes and their linked molecular markers for targeted

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introgression. Seventy two SSR markers polymorphic between the parental lines were used for background selection. results: BC3F6 basmati pyramided lines (BPL) possessing Xa21, xa13 and xa5 and their combinations were developed and evaluated for BB resistance, yield and grain quality. BPLs displayed high level of resistance to all the predominant isolates of BB pathogen prevalent in Basmati growing areas. They possessed yield and grain quality similar to Basmati parent(s) with 93-96% of RP genome resistant introgression. BC2F1 blast introgression lines possessing Pi2 and Pi54 would be crossed with BB resistant lines under convergence program. Discussions and Conclusion: BPLs of elite Basmati varieties resistant to BB are being nominated to multi-location testing under All India Coordinated Rice Improvement Programme. The BB and blast resistant Basmati lines being developed, can provide yield stability to Basmati growing farmers.

Bakanae is an important rice disease caused by Fusarium moniliforme Sheldon (Gibberella fujikuroi) that results in severe yield losses and impairs the quality of the rice grain. Studies of bakanae disease are necessary for the development of rice cultivars. Our large-scale infection method is a useful screening system which is a convenient and accurate diagnostic technique for the analysis of bakanae disease symptoms. We have also established standards for bakanae disease symptoms with which to define healthy and unhealthy plants. Bakanae disease symptoms are differentially controlled by various F. moniliforme Sheldon strains, although similar disease severity are caused specifically by the same disease strain in 10 rice varieties from 500 accessions. Three rice cultivars (Hawn, Nep Huong, and Hanareum2) expressed phenotypes resistant to F. moniliforme strain CF283 and KCC44008, and strain KCC44008 and CF283 were shown to have similar virulence. Bakanae disease symptoms are distinguishable from those induced with gibberellic acid treatment.

P0014 Large-SCaLe SCreening MeTHOD FOr eVaLUaTiOn OF BaKanae DiSeaSe in riCe 1

1

1


P0015 MOLeCULar BaSiS OF riCe- gaLL MiDge inTeraCTiOnS

1

D. Park , M. Kim , S. Lee , T. Kwon , W. 1 1 1 1 Hwang , S. Park , D. Shin , Y. Youn , J. 1 1 1 1 1 Lee , J. Cho , S. Han , U. Yeo , Y. Song , 1 M. Nam

1

2

2

D. Divya , Y. Tunginba Singh , S. Nair , 1 J.S. Bentur 1

Department of Entomology, Directorate of Rice Research-ICAR, Hyderabad, India 2 Plant Molecular Biology, International

1

Department of Functional Crop, National Institute of Crop Science RDA, Mirynag, Korea

336

Centre for Genetic Engineering and Biotechnology, New Delhi, India

between Aganni and Kavya, (HRresistance). It is thus obvious that resistance conferred by different known R genes may involve multiple/different pathways.

The Asian rice gall midge, Orseoliaoryzae is a serious insect pest causing extensive yield loss. The interaction between gall midge resistance rice varieties (possessing one of the 11 known resistance genes) and one of the seven known biotypes of the pest, is known to be on a gene-for-gene basis. Our earlier studies have shown that Gm11 gene conferred Hypersensitive-plus (HR+) type of resistance in Suraksha which is similar to rice defense reaction against pathogens. But Gm1 conferred a novel HR- type of resistance in Kavya. Here we report the molecular basis of resistance in Abhaya (with Gm4 gene) and Aganni (Gm8). Standard sets of plants involving contrasting parents and pre-near isogenic lines derived from them were exposed to avirulent populations of gall midge under controlled greenhouse conditions. Plant tissue was sampled at two time points and analyzed for differential expression of 29 candidate genes using qPCR. These genes were selected based on their map position or from analysis of suppressive subtraction hybridization (SSH) cDNA libraries developed from these rice varieties. Expression levels of the selected genes in the gall midgechallenged plants with reference to those in unchallenged plants showed over expression (≥2 fold) of ÔNBSLRRÕ in Abhaya and ÔProline rich protein 3Õ gene in Aganni. Overall pattern of gene expression brought out similarities in resistance mechanism observed in Abhaya and Suraksha. However, no distinct similarity was seen

P0016 iDenTiFiCaTiOn, MOLeCULar MaPPing anD MarKer-aSSiSTeD inTrOgreSSiOn OF nOVeL BaCTeriaL BLigHT reSiSTanCe geneS in riCe 1

2

R. Sundaram , G.S. Laha , B.C. 3 4 Viraktamath , M.S. Madhav , S.M. 4 4 5 Balachandran , N.S. Rani , T. Ram , P. 6 7 8 Natarajkumar , K. Sujatha , S.K. Hajira , 9 8 A. Yugander , K. Pranathi , C.H. 8 Balachiranjeevi 1

Biotechnology Laboratory, Directorate of Rice Research-ICAR, HYDERABAD, India 2 Plant Pathology Crop Protection Section, Directorate of Rice ResearchICAR, HYDERABAD, India 3 Crop Improvement Section, Directorate of Rice Research-ICAR, HYDERABAD, India 4 Biotechnology Laboratory Crop Improvement Section, Directorate of Rice Research-ICAR, HYDERABAD, India 5 Plant Breeding Crop Improvement Section, Directorate of Rice Research, Hyderabad, India 6 Plant Sciences, University of CaliforniaDavis, Davis CA, USA 7 Genetics and Plant Breeding, College of Agriculture Aswaraopet Acharya NG Ranga Agricultural University, Aswaraopet Andhra Pradesh, India

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Biotechnology Laboratory Crop Improvement Section, Directorate of Rice Research, Hyderabad, India 9 Plant Pathology Crop Protection Section, Directorate of Rice Research, Hyderabad, India


as a new variety, Improved Samba Mahsuri (ISM), while lines of ISM possessing Xa33 and other novel genes are under evaluation. We are also studying the diversity and virulence spectrum of the bacterial blight pathogen across India to identify durable resistance gene combinations.

With an objective of developing durable bacterial blight (BB) resistant rice varieties, we have identified, characterized and utilized novel resistance genes from wild relatives of Oryza along with known major resistance genes.

Bacterial blight resistant versions of Samba Mahsuri possessing the above mentioned resistance genes and possessing durable resistance to BB can provide yield stability to farmers.

Through wide-hybridization, new resistance genes were identified and transferred from wild species and other donors to susceptible varieties. Mapping populations developed from introgression lines were used for molecular mapping of the new resistance genes. Marker-assisted backcross breeding (MABB) was followed for targeted introgression of known and newly resistance genes into the elite variety, Samba Mahsuri.

P0017 iDenTiFiCaTiOn anD CHaraCTerizaTiOn OF TaL eFFeCTOrS (TaLeS) reSPOnSiBLe FOr VirULenCe in Xa13COMPaTiBLe inDian iSOLaTeS OF THe riCe BaCTeriaL BLigHT PaTHOgen, XanTHOMOnaS Oryzae PV. Oryzae (XOO) 1

2

3

R. Sundaram , G.S. Laha , R.V. Sonti , 4 A.J. Bogdanove

A new, broad spectrum resistance gene, Xa33 has been identified from an accession of O. nivara and fine mapped on Chr. 7. Another new gene, xaOr has been identified from an accession of O. rufipogon and mapped on Chr 1. BB resistance in Ajaya was identified to be controlled by two recessive genes, xa5 and a novel gene on Chr. 8, named xaAj. We have introgressed Xa33, xaOr xaAj, xa5, xa13 & Xa21 into the genetic background of Samba Mahsuri through MABB. One of the breeding lines possessing Xa21, xa13 and xa5 has been released for commercial cultivation

1

Biotechnology Laboratory, Directorate of Rice Research-ICAR, HYDERABAD, India 2 Crop Protection, Directorate of Rice Research-ICAR, HYDERABAD, India 3 Group Leader-Genetics, Centre for Cellular and Molecular Biology, HYDERABAD, India 4 Plant Pathology and Plant Microbe Biology, Cornell University, Ithaca, USA Many Indian isolates of the rice bacterial blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo) are compatible with rice

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genotypes possessing the major resistance gene, xa13. The present study was carried out to identify and characterize the pathogen transcription activator-like effectors (TALEs) associated with this compatibility. Four isolates of Xoo compatible with xa13 and belonging to four different Indian pathotypes (IXO93, IXO141, IXO222 and IXO620) were analyzed. The number of TALEs in each isolate was estimated through RFLP and the isolates were analyzed for their induction of known, major susceptibility genes after inoculation to IRBB13. TALE encoding genes were cloned from two geographically distinct isolates IXO93 and IXO141 and putative rice gene targets of these TALEs were identified in-silico. Targets were validated through heterologous expression of the TALEs in ME2, a nonvirulent strain of Xoo, and subsequent inoculation to IRBB13.The four isolates each possessed 14-20 TALEs and significantly induced Os11N3 (a known rice susceptibility gene responsible for computability with xa13). A total of four TALEs cloned from IXO93 and two cloned from IXO141 were predicted to target Os11N3. When delivered through ME2, from IXO93, only tal26aIXO93 (identical to AvrXa7) induced Os11N3 and resulted in infection, while tal28aIXO141 (variant of Pthxo3) and tal26bIXO141 (variant of AvrXa7) from IXO141, induced Os11N3 and resulted in infection. The study indicates that the predominant mechanism in overcoming xa13 is activation of Os11N3 and multiple TALEs have evolved to effect the activation. The study also provides insights that will

guide the choice of deployment of appropriate rice resistance genes. P0018 riCe PHenyLaLanine aMMOnia LyaSe 4 gene (OSPaL4) iS aSSOCiaTeD WiTH BrOaD SPeCTrUM DiSeaSe reSiSTanCe 1

2

B.W. Tonnessen , P. Manosalva , J. 1 1 3 Lang , J. Stephens , M. Baraoidan , A. 3 4 5 Bordeos , J. Oard , S. Hulbert , H. 3 1 Leung , J.E. Leach 1

Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, USA 2 Plant Research, Boyce Thompson Institute, Ithaca, USA 3 Entomology and Plant Pathology, International Rice Research Institute, Metro Manila, USA 4 School of Plant Soil and Environmental Sciences, Louisiana State University, Baton Rouge, USA 5 Plant Pathology, Washington State University, Pullman, USA Most agronomically important traits, including resistance against pathogens, are governed by complex genetic loci. Determining the genes, gene combinations, or genomic regions controlling these traits would contribute to the breeding of cultivars containing durable resistance because it would provide information needed to develop molecular markers. In the last decade, several defense response (DR) genes were successfully used as good predictors and

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Dept of Rice, Tamil Nadu Agricultural University, Coimbatore, India 4 Dept of Plant molecular biology, Delhi university, Delhi, India 5 Lab#40 Dept of Plant Pathology, Tamil Nadu Agricultural University, coimbatore, India

contributors to quantitative trait loci (QTL)-based resistance against several devastating rice diseases. We identified a rice mutant with a 750 bp deletion in the second exon of a member (OsPAL4) of the phenylalanine ammonia lyase gene family. OsPAL4 clusters with three other OsPAL genes that co-localized with a disease resistance QTL on rice chromosome 2. The ospal4 mutant lines exhibited increased susceptibility to bacterial blight and sheath blight diseases of rice. Within the mutant line, expression differences were detected for other OsPAL gene family members, including up-regulation of OsPAL2, which is in the same gene cluster as OsPAL4. These results suggest that OsPAL4 is an important contributor to broad-spectrum resistance in rice and argue its role in the disease resistance governed by the chromosome 2 QTL. Our results unveil OsPAL4 as a target for improving broadspectrum disease resistance in rice.

Rice is one of the important cereal crops. Tungro disease continues to pose a threat for rice production in South and Southeast Asia. Tungro incidence was recorded in farmersÕ fields in Southern districts of Tamil Nadu resulting considerable economic losses. Though management strategies have been reported in India, its impact has not reflected in real sense. Development of Tungro resistant cultivars has been major objective of rice improvement programs. RNA interference (RNAi) is principle in some of successful transgenic resistance against plant viruses, pathogen-derived resistance. In order to obtain transgenic resistance against RTBV, Pusa Basmati-1 was transformed to express the senseantisense construct of the ORF IV of RTBV (Tyagi et al.,2009) of an Indian isolate of RTBV at the University of Delhi.With the aim of diversifying the transgenic resistance of RTBV from Pusa Basmati-1 into agronomically superior but tungro susceptible varieties ASD 16, BPT 5204 & CR 1009 by backcrossing breeding programme was taken up at Paddy Breeding Station, TNAU. In each generation, RTBV resistant transgene positive plants were forwarded to the next generation. The advanced breeding lines of BC3F4 generation of cultivars viz., ASD 16, CR 1009 & BPT 5204 were phenotyped against RTD by Test tube

P0019 Phenotyping of transgenic lines of indian rice cultivars against rice Tungro Disease 1

2

P. Valarmathi , R. Velazhahan , S. 3 3 3 Manonmani , S. Suresh , S. Robin , I. 4 5 Dasgupta , R. Rabindran 1

Lab#40 Dept of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, India 2 Dept of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, India

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inoculation method by Hibino et al.,1990 at IRRI. The transgenic lines exhibited reduced tungro symptoms than the normal lines. The Real Time PCR result also revealed a positive note confirming the efficiency of the transgene imparting resistance against RTD.The transgenic lines were found to be agronomically superior comparing with the respective normal lines.

complete randomized statistical design and infection was carried out by using dry leaves spore suspension after two weeks of field establishment.The required high humidity and moisture level were maintained by covering the entire field to create moisture chambers. Results Total of 15 varieties that were used for the phenotypic screening were susceptible to the fungal infection.The rest of the varieties remained resistant to the rice blast .Tetep, Bg359, Bg304, IR64, Bg352, Bg357 were highly resistant to the rice blast disease. LD125, Bg94/1, Bw276-3, Bw451, Bw400, H7, Bg34-6, H10, IRRI119 were highly susceptible.Some of varieties H7, H10, Bg403, Bw78, Bw400, Bw276-3 have lost their resistance in comparison with previous study.

P0020 PHenOTyPiC SCreening OF SriLanKan riCe VarieTieS FOr BLaST reSiSTanCe 1

W.A.D. Jayawardana , G.A.U. 1 1 Jayasekera , R.L.C. Wijesundara , 2 D.M.N. Dissanayake 1

Department of Plant Sciences, University of Colombo, Colombo, Sri Lanka 2 Department of agrotechnoloy, Ministry of Agriculture, Colombo, Sri Lanka

Discussion

and

conclusion

The lost of resistance of some varieties were due to the emergence of virulent races with in pathogen population and narrow genetic diversity for blast resistance within in these varieties.The current study was carried out to develop F2 population for marker aided selection of rice blast resistant genes in Srilankan rice germplasm.

Introduction Rice blast is a serious disease affecting rice growing regions in Srilanka and in world,caused by the fungal pathogen Pyricularia grisea .The use of resistant varieties is considered to be the best way to control the rice blast disease.

Key wordsPyricularia grisea, phenotypic screening, blast resistance.

Methodology The selected 34 rice varieties were established in the field of rice research and development institute,Batalagoda upland blast nursery according to a

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P0021

other prefectures. Avirulence gene of Avr-Pi9, Avr-Piz and Avr-Pizt were observed at the highest frequency in blast population from 11 prefectures, and their average frequency achieved more than 80%. Our results implied that genetic diversity of rice is more important than other environment condition. According to average frequencies, the avirulence genes could be categorized into 4 groups. And there was significant difference of frequencies of avirulence genes among different groups, while insignificant difference was observed within the same group. These results will provide useful information for evaluation of evolution avirulence genes, interaction between ariculence genes and resistance genes and effectively management of rice blast disease.

geOgraPHiC DiSTriBUTiOn OF aVirULenCe geneS in MagnaPOrTHe Oryzae in yUnnan PrOVinCe, CHina 1

2

1

1


3

C. Li , J. Li , J. Yang , L. Liu , Y. Fukuta , 1 Y. Zhu 1

Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, China 2 Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China 3 Japan International Research Center for Agricultural Sciences(JIRCAS), Japan International Research Center for Agricultural Sciences(JIRCAS), Ibaraki, Japan

P0022

Knowledge of geographic distribution and frequency of avirulence genes will contribute to develop strategies to effectively utilization the rice varieties carrying various resistances genes, including combination of varieties in mixture cropping system. In this study, we analyzed geographic distribution and frequencies of avirulence genes in rice blast fungus using samples collected from 11 prefectures of Yunnan Province, China. Total of 767 single spore isolates were assayed for pathotypes based on reaction to 20 rice blast resistance monogenic lines. The results show that frequencies of avirulence genes among 10 prefectures showed insignificant difference, except for frequencies of avirulence genes in Xishuangbanna showed significant difference with the

eXPreSSiOn PaTTern OF geneS enCODing SMaLL SeCreTeD PrOTeinS in niTrOgen STarVaTiOn MeDiUM anD DUring inTeraCTiOn OF BLaST FUngUS WiTH riCe 1

1

1

1

1

J. Yang , C. LI , L. LIU , Y. ZHU , Y. DU , 1 Z. SHI 1

Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, China Pathogen compete nutrition with host during pathogen infecting host, which reflect some important features of pathogen such as pathogen depend on

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P0023

and is sensitive to nutrition. Proteins involved in competing nutrition maybe play the role in pathogen infecting, surviving and regulating. Mechanism on what role of small secreted proteins play in pathogen competing nutrition is not still discovered. We analyzed blast genome and obtained 12 candidate genes encoding small secreted proteins with N-terminal signal peptide and no any homologous in NCBI database. The expression level of 12 genes were analyzed when blast strains cultured in complete medium, 1/10-N, nitrogenstarvation medium and at the stage of infection process using real-time RTPCR, the result showed 9 genes appeared different expression in complete medium, but their expression appeared consistent tendency in 1/10-N and nitrogen starvation medium. The gene of MGS1242 appeared great rising at 24h and 48h in 1/10-N and nitrogenstarvation medium. And 7 genes presented great rising at 24hpi and 48hpi during pathogen infecting rice, and the expression level appeared maximum at 48hpi, and began to decline at 72hpi, 7 genes showed up-regulation and were up to one hundred fold. Conclusively, these genes involved in interaction of blast fungus with rice, and appeared sensitive to different nitrogen levels, which indicated expression of pathogenicity-related genes was influenced by nitrogen source in environment. And our results proved these genes without known domain were combined-action factors that were induced when blast strains cultured in nitrogen starvation medium and interacted with rice.

DiSSeCTing THe COrreLaTiOn BeTWeen LeaF BLaST anD PaniCLe BLaST By PHenOTyPing BC4F3 POPULaTiOn, MUTanTS anD MOnOgeniC LineS 1

2

1

3

J. Yang , S. Zhang , X. Zhu , E. Ardales , 4 2 L. Hei , B. Liu 1

Rice Disease, Plant Protection Research Institute Guangdong Academy of Agricultural Sciences, Guangzhou, China 2 Rice Breeding, Rice Research Institute Guangdong Academy of Agricultural Sciences, Guangzhou, China 3 Crop Protection Cluster College Agriculture, University of the Philippines Los Baños, Los Banos, Philippines 4 PBGB, International Rice Research Institute, Los Banos, Philippines Although panicle blast (PB) is more destructive than leaf blast (LB) (Magnaporthe oryzae) in rice, most of the studies are mainly focused on LB. However, inconsistent resistance to LB and PB for a given rice cultivar is always observed in rice production, implying that the mechanisms of resistance may be different. Two populations derived from a rice variety SHZ-2 with durable blast resistance were used to determine the degree of correlation between LB and PB. The two populations consist of a) 218 BC4F3 lines developed by backcrossing SHZ-2 to TXZ-13 (susceptible recurrent parent), and b) 283 EMS-induced SHZ-2 mutants. The two populations were evaluated in blast nurseries in Guangdong Province and IRRI. Significant correlations were found

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between the LB and PB in both BC4F3 population (r=0.44) and EMS mutants(r=0.79). At the same time, 24 rice blast monogenic lines were tested in greenhouse by single isolate inoculation. Our results showed that 21 of 24 rice blast monogenic lines showed consistent disease reactions to LB and PB, while 3 lines carried Pi5(t), Pi3 and Pii exhibited inconsistent reactions to LB and PB. The results suggest that LB and PB are partially correlated. Some Pi genes showed consistent effects on both LB and PB, but some did not. Thus, the degree of correlation for a given variety depends on the Pi genes carried. It is necessary to evaluate PB in addition to LB in rice breeding program. Key words: rice; leaf blast; panicle blast; correlation

marker targeting the 25 bp deletion at 18 bp upstream of the putative TATA box and assayed the allelic status at Xa 27 in 572 rice genotypes. The PCR products with 171 bp band might have the 25 bp at the promoter TATA box site, scored as type ? allele. Type ?allele was observed in high frequency of 39.7% in all accessions, which included 226 cultivars, 1 wild rice. The products of 164 accessions were a smaller fragment, hereafter referred as scored as type ?allele (approximate 146 bp). The 149 bp band pattern (Type ?allele) was unique to the IRBB27. The five O. rufippgon and one O. barthii with 160 bp band were observed and taken as type ?, two O. barthii had the type ? allele amplified 120 bp fragment. Our experiment data suggests that136 cultivars, 12 O. glaberrima and 24 wild relatives were not able to PCR amplify as Nipponbare. The alleles evaluation of the Xa 27 locus obtained by the 191 cultivars tested may contribute to little information of the resistance specificity associated with allele diversity. But we found allele frequency associated with complete resistance in the indica were different from that associated with complete resistance in the japonica.

P0024 aPPLy anD aSSeSSMenT OF a MarKer FOr BaCTeriaL BLigHT reSiSTanCe gene LOCUS Xa 27 in riCe P. Yu


1

1

Chinese National Center for Rice Improvement, China National Rice Research Institute, Hangzhou, China

P0025 Molecular tagging of brown plant hopper resistance locus in rice (Oryza sativa L.)

The bacterial blight gene locus Xa27 in rice has been cloned and two striking differences in the presumed promoter raised resistance/susceptible allele expression, while the allele in Nipponbare has diverged considerably from the gene of IRBB27. In the present study, we used a previous functional

1,2

1

B. Marathi , B. Sreevani , Kshirod K. 2 3 Jena , Jhansi Laxmi V. , Satyanarayana 4 5 P.V. , Suryanarayana Y.

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significantly associated with BPH resistance contributing 40.1% and 25.7% of phenotypic variation respectively. Linkage analysis of two polymorphic markers showed significant linkage between RM19660 and RM20037 with a distance 28.5cM. Simple interval mapping has identified a major QTL between RM 19660 and RM 20037 with a LOD value of 54.39 explaining 32% of phenotypic variation. The present study revealed RM19660 and RM 20037 on chromosome 6 are linked to BPH resistance locus and could be used for marker-assisted transfer of BPH resistance into rice genotypes.

1

Institute of Biotechnology, Acharya N.G. Ranga Agricultural University, Hyderabad, India 2 PBGB, International Rice Research Institute, Los Baños 3 Directorate of Rice Research, Hyderabad, Andhra Pradesh, India 4 Andhra Pradesh Rice Research Institute, Maruteru, West Godavari 5 Agricultural Research Station, Nellore, Andhra Pradesh, India The brown planthopper (BPH) is one of the most serious pests in tropical and temperate Asia. Earlier studies documented tremendous genetic variation for BPH resistance existing in rice germplasm. A line, MTUITJ-206-7-41, derived from MTU4569/ARC6650//Bunnan///IR64 was found to be resistant to biotype 4 of BPH. Phenotypic analysis of 170 F2:3 progenies of a cross between the resistant line MTUITJ-206-7-4-1 and a BPH susceptible line MTU 3626 revealed quantitative and transgressive nature of BPH resistance. Polymorphism survey with 235 SSR markers showed 19.6% polymorphism between parents. Bulked segregant analysis was carried out in resistant and susceptible bulks made from ten extreme resistant and susceptible F2:3 families. Out of 46 polymorphic markers, two markers on chromosome 6, RM 19660 and RM 20037 amplified a resistant parental band in the resistant bulk and a susceptible parental band in the susceptible bulk. Comprehensive genotyping of individual F2 plants with both the primers followed by single marker analysis revealed that the markers RM19660 and RM20037 were

P0026 aVenUeS OF FUngiCiDaL inTerFerenCe DUring inFeCTiOn reLaTeD DeVeLOPMenT OF BiPOLariS Oryzae anD QTLS FOr riCe BrOWn SPOT 1

2

A. KOTASTHANE , T. Agrawal , P.S. 2 3 Nandanwar , U.S. Singh 1

PLANT PATHOLOGY, Indira Gandhi Krishi Vishwavidyalaya, Raipur, India 2 Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, India 3 Office of the South Asia Regional Project Coordinator New Delhi India, IRRI India Office, New Delhi, India introduction: Background/Purpose: Rice brown spot (Cochliobolus miyabeanus) is favored by drought and imbalances mineral nutrition in soil (Ou 1985). It has been associated with two major epidemics in India

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anD eVaLUaTiOn OF gerMPLaSM / BreeDing POPULaTiOn againST riCe SHeaTH BLigHT (rHizOCTOnia SOLani) DiSeaSe

(Chakrabarti 2001; Padmanabhan 1973) and still widely reported in South and South-East Asian countries (Savary et al. 2000a, Chakrabarti 2001, Reddy et al. 2010). Host plant resistance and disruption of infection process with fungicides are two of the several ways of crop protection. Methodology: Stages of appressoria production by Bipolaris fungus and its disruption by fungicides was evaluated in vitro on freshly harvested spores. CT9993-5-10-1-M / IR62266-426-2 population was analyzed for QTLs for brown spot tolerance (Lander et al., 1987). of fungicidal results: Avenues interference revealed abnormalities in Pre-penetration morphogenesis. Three QTLs (CH # 4, 6 & 8) for brown spot tolerance were identified, (LOD score of >3, explained between 9.5 and 18.4% of phenotypic variation) and high resolution map of the QTL regions was predicted in-silico. Discussions and Conclusions: Prepenetration morphogenesis of Cochliobolus spp. and fungal genes involved in these developments have been reported by several (Braun and Howard, 1994, Clay et al., 1997, Ganem et al.,2004, Horwitz et al., 1999) and chemicals preventing these stages will lead to novel strategies for controlling plant diseases. Katara et al. (2010) reported 10 QTLs some of which may be common to the results by Sato et al. (2008).

1

2

A. KOTASTHANE , T. Agrawal , U.S. 3 Singh 1

PLANT PATHOLOGY, Indira Gandhi Krishi Vishwavidyalaya, Raipur, India 2 Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, India 3 Office of the South Asia Regional Project Coordinator New Delhi India, IRRI India Office, New Delhi, India introduction: Background/Purpose: Sheath blight (ShB) disease of rice occurs in all rice production areas of worldwide (Savary et al. 2006). Under conditions favorable for development of the disease, yield losses ranging from 1 to 65% (Marchetti, 1983; Groth, 2005) and 5*10% in Asia (Savary et al. 2000) have been reported. With no genotype identified immune to sheath blight, diverse rice germplasm (Glaszmann 1987; Garris et al. 2005; Bioversity International et al. 2007,) is a critical source for identifying resistance against sheath blight. Labour intensive techniques reported earlier limits large scale screening of germplasm which necessitates a simple technique for screening gemplasm. Methodology and results: We used R solani colonized rice bran which was deposited on the rice plants at maximum tillering stage by sieving. The technique is simple, reliable and provides high contact frequency between tissues and also allows large scale inoculation and

P0027 a SiMPLe inOCULaTiOn TeCHniQUe FOr Large SCaLe SCreening

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screening of rice germplasm / breeding population against sheath blight. Discussions and Conclusions: Different inoculum types and methods have been used for studying rice sheath blight (Willocquet et al., 2000, Singh et al., 2001, Eizenga et al., 2002, Rodrigues et al., 2003, Willocquet et al., 2011), either in the field or under controlled conditions (Yoshimura and Nishizawa 1954; Gangopadhyay and Chakraborty 1982; Wasano et al. 1983; Ou 1985). We propose our simple and reproducible inoculation technique suitable for large scale screening and evaluation of germplasm against rice sheath blight disease.

and rice grassy stunt virus (RGSV), continues to be major biotic stress of rice in Asia. Emergence of new insect biotypes and pathogen populations as a result of climate change are becoming a major threat to rice production. Wild species of Oryza are valuable sources of useful genes particularly resistance to various biotic stresses. Inter-specific hybrids between cultivated rice and wild species are important to create genetic variability in the rice gene pool. Identification of new genetic sources for durable and broad spectrum resistance to these stresses is therefore essential to increase rice production and sustainability. Different wild rice species from ten different genomes were utilized as new sources for stress resistance. A total of 191 disomic introgression lines (DILs) derived from O. officinalis (CC), O. minuta (BBCC), O. latifolia (CCDD), O. australiensis (EE), O. brachyantha (FF), and O. granulata (GG) were evaluated in this study. Laguna BPH colony of the Philippines were used to evaluate for BPH, RRSV, and RGSV resistance during dry and wet seasons of 2012 in IRRI, Los Banos, Philippines. Seventeen (17) DILs from different genomes were identified and showed a broad resistance spectrum that is significantly higher than the existing germplasm with known resistance genes. Selected DILs were crossed with two recurrent parents, IR31917-45-3-2 and IR56, for further genetic studies. Development of mapping population is underway to identify novel genes for durable and broad spectrum resistance to insect and diseases and increase yield of rice.

P0028 iDenTiFiCaTiOn OF nOVeL geneTiC reSOUrCeS FOr BrOWn PLanTHOPPer, riCe raggeD anD graSSy STUnT VirUS reSiSTanCe FrOM WiLD SPeCieS OF riCe 1

1

R.B. Vinarao , J.M. Ramos , M.L. 1 1 Ballesfin , S.S. Hechanova , M.M. Del 1 2 1 Valle , R. Cabunagan , M. Balram , K.K. 1 Jena 1

Novel Gene Resources Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Banos, Philippines 2 Biotic Stress Screening Group Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Banos, Philippines Brown planthopper (BPH) which transmits rice ragged stunt virus (RRSV)

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P0029

identified. Preliminary bioassays for the pyramided lines with different R genecombinations revealed different level of resistance reaction when challenged with virulent races/biotypes of BB, blast and BPH populations of Philippines. These backcross breeding lines with multiple R genes will pave the way to tackle the damage caused by major pests.

Development of pyramided lines with multiple resistance genes in indica rice 1


1

S.S. Hechanova , J.M. Ramos , R.B. 1 1 1 Vinarao , M.M. Del Valle , M. Balram , 1 K.K. Jena 1

Novel Gene Resources Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Banos, Philippines

P0030 PrODUCTiOn OF near-iSOgeniC LineS FOr BPH reSiSTanCe geneS in inDiCa-TyPe riCe VarieTy, ir24

Bacterial blight, blast and brown planthopper (BPH) are major biotic stresses that are severely affecting rice production in South and Southeast Asia. Therefore, breeding for durable and broad spectrum of disease and insect pest resistance in rice is essential to reduce yield losses., We transferred three resistance (R) genes, Xa4, xa5 and Xa21 for BB, one (Pi40) for blast and one (Bph18) for BPH from a japonica breeding line IR 90751-1-14-12 into two elite indica varieties (NSICRc222 and IR72) genetic background through marker-assisted backcross breeding. Initially, F1 plants with confirmed multiple R genes in heterozygous state were backcrossed with the respective recurrent parents and a set of segregating backcross progenies were developed. DNA markers linked to each resistance gene were used for foreground selection. The results revealed the presence of different R gene combinations in the backcross progenies of the two elite cultivars. Selected pyramided lines of BC2F2 progenies were analyzed and some lines with multiple R genes were

1

1

2

K.K. Jena , H. Verdeprado , D. Fujita , 1 1 3 S.S. Hechanova , S. Sierra , D.S. Brar , 4 P.S. Virk 1

Novel Gene Resources Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Banos, Philippines 2 Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Banos, Philippines 3 School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana141004 Punjab, India 4 Crop Development, HarvestPlus, Hyderabad, India Brown planthopper (Nilarparvata lugen Stal, BPH) is one of the most destructive insect pests in rice. Development of Near-Isogenic-Lines (NILs) is an important strategy for genetic analysis of BPH resistance genes and their deployment. In this study, we developed

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functional polymorphisms in a disease resistance gene

fifteen NILs derived from nine resistant donors. These NILs carried bph2, Bph3, bph4, Bph9, Bph10, Bph17, Bph18, Bph20, Bph21, Bph20+Bph21, Bph25, Bph26, Bph25+Bph26 andQbph6, and Qbph4+ Qbph6 in the genetic background of the susceptible indica variety, IR24 through marker-assisted selection (MAS). Known linked markers for each of the resistance genes and QTLs used for foreground selection revealed that chromosome segments were introgressed in the regions where BPH resistance genes were mapped. A total of 307 SSR and 18 STS markers used for background selection showed that most of the chromosome region of each NILs were IR24-type. BPH population from Laguna, Philippines was used to evaluate the insect resistance of the NILs and their respective donor parents. BPH resistance of NILs and donor parents were not significantly different except for NILs with single gene for Bph20, Bph21, Bph25, Bph26, Qbph6. Overall, low phenotypic variations for morphological traits among NILs and IR24 were observed. However, highly significant differences in panicle length of NILs and recurrent parent were noted. These newly developed NILs will be useful not only as genetic tools for BPH resistance study but could be a good source of genes against emerging biotypes of BPH for genetic improvement of rice.

1

2

S. Fukuoka , S. Yamamoto , R. 1 1 Mizobuchi , N. Kitazawa , U. 1 3 4 Yamanouchi , N. Yasuda , Y. Fujita , 5 1 T.T.T. Nguyen , K. Sugimoto , S. 6 7 1 Koizumi , T. Matsumoto , M. Yano 1

Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Japan 2 Genetic Resources Center, National Institute of Agrobiological Sciences, Tsukuba, Japan 3 Agricultural Research Center, National Agriculture and Food Research Organization, Tsukuba, Japan 4 College of Bioresource Sciences, Nihon University, Fujisawa, Japan 5 Science Technology and Environment, Ministry of Agriculture and Rural development, Hanoi, Vietnam 6 Tsukuba International Center, Japan International Cooperation Agency, Tsukuba, Japan 7 Agriculture Forestry and Fisheries Research Council, Ministry of Agriculture Forestry and Fisheries of Japan, Chiyoda-ku, Japan Quantitative trait locus (QTL) for resistance to blast offers a potential source for durable resistance of rice. To gain an understanding of its molecular basis, we cloned Pi35 gene on chromosome 1, whose resistant allele is from a breeding line Hokkai 188. Fine genetic mapping in a population derived from a cross between the Hokkai 188 and a susceptible cultivar DanghangShali delimited the Pi35 locus within a 78-kb interval on a susceptible Nipponbare genome. Comparing DNA

P0031 Map-based cloning of Pi35, a gene for quantitative trait locus controlling blast resistance, identifies multiple

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against Sogatella furcifera (Horvath) made at Paddy Breeding Station (Department of Rice), Tamil Nadu Agricultural University, Coimbatore indicated that PTB 41 was found to be moderately resistant in Seedling Screening technique (SST) and resistant under modified seedling screening technique (MSST) PTB 41 registered more feeding punctures, lower feeding rate, lowest nymphal emergence, higher percentage of unhatched eggs ,lower population buildup, longer nymphal duration and shorter adult longevity The population build-up and lower functional plant loss index compared to TN1 . Stem solidness, tightly packed cells of parenchyma, sclerenchyma and thick epidermis were found to be higher in resistant genotypes compared to TN1. Total chlorophyll content was maximum in leaf blade than in leaf sheath. The induction of peroxidase and polyphenol oxidase was the highest at 1day after infestation while it was 3 days after days for catalase and persisted for a week. There were five, three and two unique isoforms pattern of peroxidase, polyphenol oxidase and catalase, respectively. PTB 41 was also found to be resistant to BPH and GLH .PTB 41 can be used as donor like PTB 33 in future against BPH.

sequences of a BAC clone containing the Pi35 and that of Nipponbare identified an allele of the Pish as the candidate and complementation testing for this gene confirmed improvement of blast resistance. Deduced amino acid sequences of the Pi35 have differences from the Pish in both of nucleotidebinding site (NBS) and leucine-rich repeat (LRRs) regions. Replacing the LRR region of Pish with those of the Pi35conferred partial resistance against multiple fungal isolates, although this resulted loss of Pish-mediated racespecific resistance. The level of resistance of the lines that carries this chimeric gene was not as high as that with thePi35, indicating that variations in both regions are necessary for Pi35mediated resistance. Our result suggests allelic variation in NBS-LRR genes can be potential source for improving QTL-mediated disease resistance. P0032 HOST PLanT reSiSTanCe againST riCe WHiTeBaCKeD PLanTHOPPer, SOgaTeLLa FUrCiFera (HOrVaTH) S. Seetharaman


1

1

Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, India Studies on the identification of resistant sources based on nymphal preference, fecundity, nymphal emergence, egg unhatchability, nymphal duration, adult longevity, feeding marks, feeding rate, population build-up, functional plant loss index and, biochemical analysis

P0033 reSiSTanCe OF PHiLiPPine reLeaSeD riCe VarieTieS TO BaCTeriaL BLigHT 1

1

M. Burgos , I. Oña , C. Vera Cruz 1

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Plant Breeding Genetics and

1

Biotechnology, International Rice Research Institute, Los Baños, Philippines

resistant rice varieties carrying multiple or novel Xa genes associated with resistance can serve as good donors in future breeding programs. Varieties with BSR to BB are currently being evaluated for resistance to multiple rice diseases.

The availability of varieties with multiple resistance to diseases is essential to farmers to reduce yield losses in rice production. An important component in the breeding program, incorporation of multiple resistance to diseases also provides breeders with elite lines/varieties to select from when identifying the best parents to combine with other target traits. In search for possible donors and recipients for improving disease resistance, 139 rice varieties developed at IRRI and the Philippine Rice Research Institute were evaluated for resistance to BB by a combination of confined phenotypic evaluation using 10 diagnostic Xoo races consisting of 14 strains and genotyping with gene-based markers for Xa4, xa5, Xa7, xa13, and Xa21. Most of the varieties carry Xa4 (80.5%) while 5% of them carry xa5. Two varieties, NSIC Rc222 and NSIC Rc286 have combined Xa4+xa5 and Xa4+Xa21, respectively, and showed broadspectrum resistance (BSR) against all Xoo strains. The irrigated varieties PSB Rc82, NSIC Rc154, and NSIC Rc238, saline prone varieties - NSIC Rc184, NSIC Rc190, and a rainfed variety - NSIC Rc274 showed broadspectrum resistance to 810 Xoo races. Interestingly, they may carry novel R genes for BB resistance in addition to the Xa gene(s) detected. Further, different varieties showed varying levels of resistance and susceptibility to particular strains. Combined with superior traits, these

P0034 CySTeine PrOTeaSe gene FrOM BraSSiCa raPa iMPrOVeS reSiSTanCe TO BaCTeriaL LeaF BLigHT in TranSgeniC riCe 1

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1

M.C. Nino , S.E. Abdula , H.J. Lee , D.A. 1 1 1 Yu , S.U. Byeon , F.C. Nogoy , K.K. 3 4 1 Kang , I.S. Nou , Y. Cho 1

Crop Science, Chungbuk National University, Cheongju, Korea 2 Plant Breeding and Biotechnnology, Philippine Rice Research Institute, Midsayap, Philippines 3 Horticulture Science, Hankyong National University, Ansung, Korea 4 Horticulture Science, Sunchon National University, Sunchon, Korea Bacterial blight is a serious problem of rice in irrigated and rainfed lowlands. It is caused by Xanthomonas oryzae pv. oryzae (Xoo) which is represented by many pathotypes, making it difficult to control. Plant proteases are important players in immunity acting either in the execution of attack, in signaling cascade or in perception of invader. This study demonstrates the response of cysteine protease (CP) upon interaction with the pathogen. The cysteine proteaseencoding full-length cDNA was identified and characterized using web-based

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Department of Agriculture, Regional Field Unit I, La Union, Philippines

tools. This gene is 2,267 bp in size with an open reading frame of 1,365 bp that encodes predicted polypeptide of 455 amino acids. The conserved domain of the gene revealed its affinity to Peptidase_CIA family. The full-length cDNA of CP in Brassica rapa was then cloned and overexpressed in rice. Insertion of gene was verified in the transformants through genomic PCR assay. Spatiotemporal expression of the gene was performed in transgenic rice. To evaluate the resistance of CPoverexpression lines to Xoo, transgenic plants were inoculated with two races of Xoo. In plantaanalysis of enzymatic activity of CP was also performed before and after infection by the pathogen. This research was supported by iPET (111098-3), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. *Corresponding author: [email protected]

The first generation of improved lines with bacterial blight resistance gene was carried out successfully, but there were difficulties in converting them into cytoplasmic male sterile lines. This is due to strong fertility restoration of resistance donor parent when crossed to wild abortive cytoplasm. In this study, we sought to develop a new generation maintainer line with two or more multiple resistance genes with no residual fertility effect in converted CMS lines, and with good grain quality. Fifty one maintainer lines assembled at PhilRice were screened by inoculating 13 Xanthomonas oryzae pv. oryzae isolates and confirmed what possible genes were present using gene markers. The parent lines used were IRBB62 and identified maintainer lines showing broader resistance to bacterial blight but with poor morpho-agronomic traits. Grain quality profiles of the selected parent lines were also considered. A combination of forward and marker assisted breeding was used in the breeding process. After six generations, ten advanced lines were already uniform and confirmed having two to three bacterial blight resistance genes. Using four cytoplasmic sources, pollen sterility evaluation of BC1F1 progenies of these advanced lines revealed different reactions to iodine staining. Grain quality of these advanced lines exhibited acceptable value for percent chalkiness, head rice, and amylose content. Therefore, with appropriate selection of cytoplasmic source, the development of CMS lines without fertility residual effect is possible.

P0035 geneTiC iMPrOVeMenT OF MainTainer LineS FOr MULTiPLe BaCTeriaL BLigHT reSiSTanCe geneS anD gOOD grain QUaLiTy FOr PHiLiPPine HyBriDS 1

1

1

I.G. Pacada , F.P. Waing , J.J. Unay , 2 3 C.P. Quinones , L.P. Coloma , D.A. 1 Tabanao 1

Plant Breeding and Biotechnology, Philippine Rice Research Institute, Science City of Muñoz, Philippines 2 Plant Breeding Genetics and Biotechnology, International Rice Research Institute, Los Baños, Philippines

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Moreover, thorough and strict selection of parent lines will further improve maintainer lines gene pool for bacterial blight resistance gene and excellent grain qualities.

BB resistance is largely absent in the rice germplasm currently used in Africa. Our goal is to identify novel, broadspectrum resistance sources to control BB and BLS in rice. We are using a new mapping resource called a Multi-parent Advanced Generation Inter-Cross (MAGIC) population to facilitate identification and mapping of new resistance. The MAGIC population was developed using eight elite indica cultivars with diverse genetic backgrounds as founders. The advantage of MAGIC populations is the increased level of recombination and the precision and resolution to detect QTL. Furthermore, interactions among multiple genomes may lead to transgressive segregation resulting in enhanced disease resistance. In screens with African strains of Xoo and Xoc, the founders exhibited reproducible differential responses, suggesting that they carry different sources of resistance. At the fourth selfed generation (S4), 200 single seed descent lines were genotyped by sequencing. The genotyped collection has been phenotyped for BB and BLS resistance. Genome-wide association analysis will be used to map disease resistance QTL. Because the MAGIC population was derived from elite cultivars, its use in breeding programs will be expedited.

P0036 iDenTiFiCaTiOn OF nOVeL reSiSTanCe SOUrCeS FOr BaCTeriaL DiSeaSeS in riCe USing a MULTi-ParenT reCOMBinanT POPULaTiOn 1

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A.M. Bossa-Castro , C. Raghavan , E.E. 1 2 2 Delorean , C.M. Vera Cruz , H. Leung , 3 4 1 G. Mosquera , V. Verdier , J. Leach 1

Bioagricultural Sciences, Colorado State University, Fort Collins, USA 2 Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Manila, Philippines 3 Agrobiodiversity Program, International Center for Tropical Agriculture, Cali, Colombia 4 Plant Pathology, Institut de Recherche pour le Développement, Montpellier, France Bacterial blight (BB) and bacterial leaf streak (BLS) caused by the pathogens Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), respectively, are widespread diseases of rice in Africa and Asia. In Africa, BLS is an emerging disease, causing considerable losses. So far there is no known source of single gene resistance for Xoc. While many BB resistance genes are known, effective

P0037 14-3-3 PrOTeinS (gF14B anD gF14e) FUnCTiOn in PaniCLe BLaST reSiSTanCe in riCe

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2

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Transgenic Nipponbare overexpressing GF14b and GF14e showed enhanced resistance to panicle blast. Conversely, transgenic rice silenced for GF14b and GF14e were more susceptible to panicle blast. The over-expression of GF14b and GF14e were accompanied by the suppression of a LOX (lipoxygenase, D14000) gene after inoculation. In addition, the expression of GF14b and GF14e were significantly suppressed in rice EMS mutants selected for loss of panicle blast resistance. Our results indicate that GF14b and GF14e play important roles in panicle blast resistance, and point to key novel differences between leaf and panicle blast resistance. Further studies on the mechanisms by which these two 14-3-3 proteins contribute to resistance are under way.

Q. Liu , A.Q. Feng , J.L. Zhao , S.H. 1 2 2 Zhang , J.Y. Yang , X.Y. Zhu , J.E. 3 4 1 Leach , H. Leung , B. Liu 1

Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Rice Research Institute Guangdong Academy of Agricultural Sciences, Guangzhou, China 2 Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China 3 Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, USA 4 Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Metro Manila, Philippines Although 14-3-3 proteins are known to negatively regulate leaf blast resistance in rice, their involvement in resistance to panicle blast, a more destructive type of rice blast disease, has not been reported. To confirm the functions of 14-3-3 proteins during panicle blast disease and resistance, we used the Agilent rice 44K oligonucleotide microarray and realtime PCR to analyze differential gene expression in a resistant advanced backcross line (BC10) and its susceptible recurrent parent (Texianzhan 13) after challenge with the blast pathogen. The functions of differentially expressed genes in panicle blast resistance were confirmed by gene overexpression and silencing analyses. GF14b and GF14e, two 14-3-3 protein family members, were down-regulated during leaf blast disease, but were induced during panicle blast disease. GF14b and GF14e also were highly expressed in the spikes of rice plants.

P0038 aSSOCiaTiOn MaPPing OF reSiSTanCe TO BaCTeriaL BLigHT (XanTHOMOnaS Oryzae PV. Oryzae) in DiVerSe riCe gerMPLaSM 1

1

C.J. Dilla-Ermita , E.S. Tandayu , D. 1 1 1 Lozada , L. Nora , V. Bartolome , C. 1 2 3 Vera Cruz , E.Y. Ardales , M.G. Diaz , 3 4 M.S. Mendioro , M.J. Thomson 1

Plant Breeding Genetics and Biotechnology, International Rice Research Institute, Los Baños, Philippines 2 Crop Protection Cluster, University of the Philippines Los Baños, Los Baños, Philippines 3 Institute of Biological Sciences,

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University of the Philippines Los Baños, Los Baños, Philippines 4 Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Baños, Philippines

and PXO341 (race 10). Conclusions Known loci of resistance genes detected in this study confirm the association of these genes to resistance even in a diverse panel. Novel loci associated with differential resistance to the nine races provide alternative sources of resistance, thus sources of these loci can be included to enrich the core set for BB resistance breeding. In addition, results of this study will facilitate the development of SNP markers for marker-aided selection.

introduction The worldwide collection of rice germplasm covers a wide range of genetic diversity and serves as a reservoir of useful genetic variation for biotic stress resistance. Association analysis of bacterial blight (BB) resistance is valuable in discovering new resistance genes. This study aims to determine genes and alleles that are associated to BB resistance. Methodology About 285 diverse accessions were evaluated for resistance in three replications using nine races of Xanthomonas oryzae pv. oryzae (Xoo). Population structure analysis was determined based on 384-SNP Illumina BeadXpress data. Genome-wide association of differential resistance to the nine Xoo races was performed using genotyping-by-sequencing from the Cornell Institute of Genomic Diversity. results GLM and MLM analyses on TASSEL based on 11,378 filtered SNPs have identified associations of known resistance genes and new loci to differential resistance to the nine races of Xoo. Xa4 and xa5 loci were consistently associated with resistance to several races. Furthermore, novel loci were also found to be associated with resistance to PXO86 (race 2), PXO339 (race 9a), PXO349 (race 9b),

P0039 anaLySiS OF aLLeLiC VariaTiOnS in riCe BLaST reSiSTanCe gene Pi54 anD PaTHOgen aVirULenCe gene aVrPi54 HeLPS in UnDerSTanDing THeir COeVOLUTiOn 1

1

1

T.R. Sharma , S. Thakur , S. Ray , P.K. 1 2 3 Singh , A.K. Singh , M. Variar , U.D. 2 4 5 Singh , R. Rathour , S.K. Prashanthi , 1 N.K. Singh 1

National Research Centre on Plant Biotechnology, Indian Council of Agricultural Research, Delhi, India 2 IARI, Indian Council of Agricultural Research, New Delhi, India 3 CRRI, Indian Council of Agricultural Research, Hazariagh, India 4 Agril Biotechnology, CSHPKV, Palampur, India 5 Agril Biotechnology, UAS, Dharwad, India Rice blast caused by Magnaporthe oryzae is one of the important biotic

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P0040

stresses of rice which results in heavy yield losses throughout the world. This disease can be managed by resistance gene deployment in various rice cultivars. Rice blast resistant gene Pi54 has been cloned from rice line Tetep and is effective against diverse isolates of M. oryzae in different parts of India. To understand the effectiveness of this gene in India, we used allele mining approach to prospect allelic variants of both the resistance gene Pi54 from a set of 92 rice lines and its counterpart AvrPi54 gene from 70 isolates of M. oryzae. We obtained many single nucleotide polymorphisms (SNPs) and InDels of different sizes in all the Pi54 alleles, using comparative sequence analysis of the resistance and susceptible alleles. Based on nucleotide polymorphism of Pi54 alleles many unique haplotypes were identified. We also showed that the selection pressure was high on the nucleotide binding site (NBS) as well as on leucine rich repeats (LRR) regions in disease susceptible alleles compared to the resistance alleles. Sequence analysis of AvrPi54 alleles showed very interesting results since we did not find much variation in different regions of this gene. We concluded that since Pi54 has not yet been deployed in commercial cultivars of rice, M. oryzaepopulation is stably surviving and there is less mutation at AvrPi54 locus due to the coevolution of Pi54 and AvrPi54 under natural environment.

reCeSSiVe anD DOMinanT reSiSTanCe TO TWO TUngrO VirUSeS CO-eXiSTing in riCe CULTiVar UTri MeraH 1

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I.R. Choi , G.A. Atienza-Grande , J.H. 1 1 2 Shim , R.J.A. Macalalad , J.H. Lee , J.R. 1 1 3 Encabo , R.C. Cabunagan , K. Satoh , 1 4 I.H. Slamet-Loedin , Y. Kishima , S. 3 1 Kikuchi , H. Leung 1

Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Baños, Philippines 2 Department of Functional Crop Science, National Institute of Crop Sciences, Milayng, Korea 3 Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Japan 4 Laboratory of Plant Breeding, Hokkaido University, Sapporo, Japan Rice tungro disease is caused by the interaction between Rice tungro spherical virus (RTSV, RNA virus) and Rice tungro bacilliform virus (RTBV, DNA virus). Cultivar Utri Merah is resistant to both RTSV and RTBV, whereas cultivar Taichung Native 1 (TN1) is susceptible to the both viruses. The associations between the reactions to the two viruses and the genetic variations in populations developed between Utri Merah and TN1 were examined to identify genes involved in the resistance traits. Genetic and sequence analyses for RTSV resistance indicated that the RTSV resistance is associated with a single recessive locus (tsv1) containing a gene encoding

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translation initiation factor 4-gamma tsv1 (eIF4G , LOC_Os07g36940) in chromosome 7. Transgenic TN1 plants tsv1 in which the expression of eIF4G was suppressed were resistant to RTSV, confirming the involvement tsv1 of eIF4G in RTSV resistance. Genetic analyses for RTBV resistance indicated that RTBV resistance is controlled by two dominant loci, one (TBV1) of which was mapped within an approximately 200-kb region near 31Mb of chromosome 4. TheTBV1 locus contains two genes encoding Argonautes (OsAGO2 (LOC_Os04g52540) and OsAGO3 (LOC_Os04g52550)) involved in gene silencing. Methylation analysis of the RTBV genome showed that parts of the RTBV genome is more methylated in RTBV-resistant plants than in susceptible plants, suggesting the involvement of the gene silencing defense mechanism in RTBV resistance. Collectively, these results indicated that two independent virus defense mechanisms are functioning against RTSV and RTBV in Utri Merah.

The rice production and productivity worldwide is often adversely affected due to biotic and abiotic stresses. In biotic stresses, plant hoppers particularly Brown Plant Hopper, is a major pest which contribute significantly to the yield losses in India. Host plant resistance is the most preferred, economically feasible and ecofriendly approach to protect the crop from hopper damage. Andhra Pradesh Rice Research Institute, Maruteru has got distinction in development and release of several popular BPH resistant varieties like MTU5249, MTU1061,MTU1064 ) and it has unique contribution of developing mega varieties like MTU7029 and MTU1010.Maruteru is well known hotspot for plant hopper occurrence and donor involving{(Vajram /Darrington)/Vajram}/I R64 was developed using pedigree method. The identified donar was screened at both field and glass studies at maruteru and across locations by DRR and scored as per standard evaluation system. The line MTU ITJ206-7-4-1(BM71) showed plant hopper damage score Ô1Õ and in subsequent screening done in Rabi 1999 under both field and glasshouse conditions the damage score was Ô1.3Õat Maruteru and DRR,Hyderabad. Genetic analysis indicated that a single dominant gene governs resistance in BM71 (Padmavati et al., 2006 and balakrishna et al.,2012) while the resistance in PTB33 was governed by two dominant complementary genes. Keeping in view the above facts, it can be concluded that BM71 with strong resistance to plant hoppers, simple gene

P0041 MTU iTJ 206-7-4-1, a STrOng DOnOr FOr PLanT HOPPer reSiSTanCe in riCe. 1

P. Venkata satyanarayana , N. 1 1 Chamundeswari , B. Balakrishna , B. 1 1 Ravikumar , K. Vasantha bhanu , M. 1 1 Girijarani , P. Ramanarao , M. Barath 1 laxmi 1

genetics and plant breeding, Andhra Pradesh Rice Research InstituteMaruteru, tanuku, India

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inheritance coupled with good quality and high yield has immense potential in developing rice varieties with strong resistance to hoppers coupled with good quality and yield under typical tropical irrigated ecosystem.

length caused by PXO236 was less severe than PXO524 in rice nearisogenic line, IRBB8. Survey in the genome of PXO236 and PXO524 reveals at least ~4,000 protein-coding genes, including 483 secreted proteins. We were able to predict a total of 67 type III effectors, among which 7 appear to be strain-specific. To investigate which effector contributes to bacterial virulence and triggers susceptibility in rice, we detected transcript accumulation of candidate effector genes and plant defense related genes that are induced during pathogen colonization. We isolated infected tissues at 6 and 24 hours post inoculation and experimentally confirmed a group of candidate type III effectors that are induced during biotrophic colonization. The functional role of these effector genes will be investigated in more detail.

P0042 iDenTiFiCaTiOn OF MOLeCULar DeTerMinanTS OF VirULenCe DUring inTeraCTiOn OF riCe TO TWO STrainS OF XanTHOMOnaS Oryzae PV. Oryzae 1

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1

G. Grande , I. Quibod , S. Dossa , F. 2 1 2 Borja , P. Capistrano , R. Mauleon , C. 1 1 Vera-Cruz , R. Oliva 1

Plant Breeding Genetics and Biotechnology, International Rice Research Institute, Los Baños, Philippines 2 T.T. Chang- Genetic Resources Center, International Rice Research Institute, Los Baños, Philippines

P0043 genetic dissection of resistance to green leafhopper and rice tungro spherical virus in cultivar arC11554

Rice bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo), is an increasing constraint to rice production worldwide. Breeders often resort to resistant rice cultivars in managing the disease; however, the emergence of highly aggressive clones in the pathosystem limits the plant defense mechanisms. In order to gain insights in the virulence variability exist in the plant-bacterial populations, two dominant strains of Xoo were sequencecharacterized and analyzed specifically for the function of bacterial effector proteins in pathogenesis and interaction with the host. Here we observed lesion

1

2

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A. Dela Cruz , J. Lee , R. Cabunagan , 3 3 D. Fujita , G. Atienza-Grande , E. 4 1 5 Mendoza , D. Tabanao , H. Yasui , I. 3 Choi 1

Plant Breeding and Biotechnology Division, Philippine Rice Research Institute, Nueva Ecija, Philippines 2 Department of Functional Crop Science, National Institute of Crop Sciences Rural Development Administration, Gyeongnam, Korea 3 Plant Breeding Genetics and

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Biotechnology Division, International Rice Research Institute, Los Baños, Philippines 4 Institute of Plant Breeding Crop Science Cluster College of Agriculture, University of the Philippines, Los Baños, Philippines 5 Plant Breeding Laboratory Faculty of Agriculture Graduate School, Kyushu University, Fukuoka, Japan

not detected in the plants carrying only the RTSV resistance gene, whereas at least 30% of the plants carrying only the GLH resistance gene were found to be infected with RTSV. The result suggests that the use of resistance to virustransmitting insects may not assure the prevention of virus diseases in rice. P0044

Rice tungro disease is caused by Rice tungro bacilliform virus and Rice tungro spherical virus (RTSV) transmitted by green leafhoppers (GLH). Rice cultivar ARC11554 had been previously found to be resistant to RTSV and GLH. The associations of RTSV and GLH resistance with genotypic variations in populations derived from ARC11554 were examined to identify loci controlling the resistance traits. GLH resistance was found to be controlled by a single dominant locus located within an approximately 300-kb region near 4.4 Mb of chromosome 4. Identification of a locus associated with RTSV resistance was carried out using populations which are susceptible to GLH to avoid masking of RTSV resistance with GLH resistance. RTSV resistance was found to be controlled by a single recessive locus in chromosome 7 containing a nonsusceptible allele of the translation initiation factor 4-gamma gene (LOC_Os07g36940), which has been known to confer RTSV resistance in other rice cultivars. ARC11554-derived plants with different combinations of the GLH resistance gene and the RTSV resistance gene were inoculated with RTSV by GLH to assess the effectiveness of the two genes in prevention of RTSV infection. RTSV was

PCr-based Dna markers for rapid discrimination of single nucleotide polymorphisms in the translation initiation factor 4-gamma gene conferring resistance to rice tungro spherical virus 1

2

1

G. Grande , J. Lee , S. Kim , R. 1 1 1 Cabunagan , A. Dela Cruz , J. Shim , D. 1 1 Fujita , I. Choi 1

Plant Breeding Genetics and Biotechnology, International Rice Research Institute, Los Baños, Philippines 2 Department of Functional Crop Science, National Institute of Crop Sciences Rural Development Administration, Gyeongnam, Korea Rice tungro spherical virus (RTSV) is one of the two viruses causing rice tungro disease. A locus for recessive resistance to RTSV (tsv1) in cultivar Utri Merah was mapped within a 200-kb region near 22.1 Mb of chromosome 7. Sequence analyses of a gene for translation initiation factor 4-gamma tsv1 (eIF4G) located within tsv1 (eIF4G , LOC_Os07g36940) among RTSVsusceptible and -resistant cultivars indicated that single nucleotide

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DeriVeD FrOM an inDiCa riCe CULTiVar aSD7

polymorphisms (SNP) in nucleotide sequences corresponding to the amino acids 1059 and 1060 of the predicted eIF4G are associated with RTSV resistance. To define the variations tsv1 alleles associated with in eIF4G RTSV resistance, tsv1 partialeIF4G sequences surrounding the SNP were examined among more than 100 rice genotypes. The sequence survey revealed that RTSV resistance of cultivars such as Utri Merah, Utri Rajapan, ARC11554, Kinmaze, Adey Selection and TKM6 is associated with one of the 6 resistance (non-susceptible) alleles containing nucleotide substitutions or deletions different from the corresponding sequences in the susceptible allele. PCR-based DNA markers designed to discriminate the susceptible and the non-susceptible tsv1 were developed for alleles of eIF4G rapid selection of RTSV-resistant plants. The DNA markers were able to distinguish among the susceptible and the 6 non-susceptible alleles existing in 20 cultivars. Genotype examination of populations segregating for RTSV resistance with the DNA markers showed co-segregation of the reactions to RTSV with the genotypes manifested by the markers. These results demonstrated the potential usefulness of the DNA markers in marker-aided selection of rice for RTSV resistance.

1

2

T. Mai Van , A. Yoshimura , H. Yasui

2

1

Graduate School Kyushu University, Fac. of Agriculture, Fukuoka 812-8581, Japan 2 Graduate School Kyushu University, Fac. of Agriculture, Fukuoka 812-8581, Japan An accession of indica rice cultivar ASD7 was found to be highly resistant to green rice leafhopper, Nephotettix cincticeps Uhler (GRH) by an antibiosis test at seedling stage. To reveal the genetic basis of the GRH resistance in ASD7, a total of 102 F2 plants derived from a cross between a susceptible japonica cultivar, Taichung 65, and a highly resistant indica cultivar ASD7 were analyzed using 96 SSR markers. The genetic map for the F2 population was constructed using a total of 96 SSR markers distributed across the 12 rice chromosomes by MAPMAKER/Exp. 3.0. Both phenotype and genotype data were used together for QTL analysis. Composite interval mapping was conducted using QGene v4.3.10. A single major QTL (LOD score = 18.1), designated qGRH5, for GRH resistance was detected on chromosome 5, with a contribution of 54.0% to the phenotypic variation. Further mapping ofqGRH5 using 277 BC2F3 plants confirmed the location of the resistance gene and found the more tightly linked the simple sequence repeat markers RM6082, RM3381, and RM249. Near-isogenic plants (BC6F1) carrying the heterozygous chromosome segment of the targeted QTL was developed

P0045 MOLeCULar MaPPing OF a MaJOr gene COnFerring reSiSTanCe TO green riCe LeaFHOPPer, nePHOTeTTiX CinCTiCePS UHLer,

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through advanced backcrossing and marker-assisted selection. The DNA markers found to be closely linked to qGRH5 would be useful for the improvement on host-plant resistance to GRH in rice.

bacterial blight, and thus associated with defense responses. In here, we report the identification of rice lesion mimic and senescence (lms) mutant and the corresponding LMS gene harboring the mutations that are responsible for the lesion mimic phenotypes. The lms mutant shows susceptibility to cold stress, exhibits partial resistance to drought and enhanced resistance after rice blast (Magnaporthe oryzae) infection.

P0046 iSOLaTiOn anD CHaraCTerizaTiOn OF riCe LeSiOn MiMiC anD SeneSCenCe MUTanT (LMS) WiTH enHanCeD reSiSTanCe TO riCe BLaST (MagnaPOrTHe Oryzae) 1

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P0047 DeVeLOPMenT OF near iSOgeniC LineS FOr BLaST reSiSTanCe in BaSMaTi riCe

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J. Undan , M. Tamiru , A. Abe , H. 2 1 2 Takagi , J.Q. Undan , H. Yaegashi 1

1

Biology, Central Luzon State University, Science City of Munoz, Philippines 2 Genetics and Genomics, Iwate Biotechnology Research Center, Kitakami, Japan

1

A. Khanna , A.B. Shikari , S. Gopala 1 2 3 Krishnan , U.D. Singh , T.R. Sharma , R. 4 5 6 Rathour , M. Variar , S.K. Prashanti , 1 A.K. Singh 1

Division of Genetics, Indian Agricultural Research Institute, New Delhi, India 2 Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India 3 Indian Agricultural Research Institute, National Research Centre on Plant Bioechnology, New Delhi, India 4 Department of Agricultural Biotechnology, CSHPKV, Palampur, India 5 Central Rainfed Upland Rice Research Station, CRRI, Hazaribag, India 6 Department of Biotechnology, University of Agricultural Sciences, Dharwad, India

Mutants that show spontaneous cell death or necrotic lesions phenotype in the absence of pathogen and abiotic stresses are commonly called lesion mimic mutants (LMMs). A number of LMMs have been identified in multiple crop species, so far, only few of the genes associated to LMMs phenotypes are already characterized and being studied in details. Based on the identity of these genes, it has been suggested that many of the lesion mimic mutants are involved in plants defense mechanism that alters its physiology resulting to cell death in response to biotic and abiotic stresses. LMMs are also known to confer resistance to multiple isolates of rice blast and

introduction: Basmati rice is popular worldwide owing to its unique grain and

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cooking quality traits. Basmati rice varieties in general and Pusa Basmati 1, one of the most popular semi-dwarf Basmati rice variety is prone to various biotic stresses, among which rice blast caused by Magnaporthe grisea is one of the most damaging diseases causing detrimental effects on grain yield as well as quality. Methodology: DHMAS70Q 164-2a, IRBL9-W, IRBL5-M, IRBLb-B and IRBLz5-CA were used as donors to incorporate blast resistance genes, Pi54, Pita, Pi1, Pi9, Pi5, Pib, Piz5 into Pusa Basmati 1. Foreground selection was carried out using gene linked/ gene based markers followed by rigorous phenotypic selection for agronomic and cooking quality, background selection including the markers linked to genes/ QTLs for quality traits in order to accelerate the reconstitution of recurrent parent phenome and recombinant selection to eliminate linkage drag. results: A set of NILs for seven blast resistant genes was developed in the background of Pusa Basmati 1. The recovery of recurrent parent genome in the NILs was more than 95.0%. Evaluation of agronomic performance and disease resistance confirmed the effectiveness of the NILs in managing blast disease. Discussion and Conclusion: Since, there was no resistance available in the Basmati germplasm, the NILs for seven blast resistant genes with Basmati grain quality would help in managing the disease in Basmati rice as the lines found promising can be released as new cultivar with in-built resistance while they would also serve as valuable donors for blast resistance genes.

MarKer aSSiSTeD PyraMiDing OF MaJOr BLaST reSiSTanCe geneS IN BASMATI RICE VARIETY ‘PUSA BASMATI 1’ 1

1

A.B. Shikari , A. Khanna , S. Gopala 1 2 3 Krishnan , U.D. Singh , T.R. Sharma , R. 4 5 6 Rathour , M. Variar , S.K. Prashanti , 1 1 K.V. Prabhu , A.K. Singh 1

Division of Genetics, Indian Agricultural Research Institute, New Delhi, India 2 Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India 3 Indian Agricultural Research Institute, National Research Centre on Plant Bioechnology, New Delhi, India 4 Department of Agricultural Biotechnology, CSHPKV, Palampur, India 5 Central Rainfed Upland Rice Research Station, CRRI, Hazaribag, India 6 Department of Biotechnology, University of Agricultural Sciences, Dharwad, India introduction: Basmati rice in general and Pusa Basmati 1, a popular semidwarf Basmati rice variety in particular is highly susceptible to rice blast caused by Magnaporthe. Therefore, the present study was undertaken with the objective to incorporate three major genes namely, Pi54, Pi1 and Pita in the genetic background of Pusa Basmati 1. Methods: DHMAS70Q164-2a harboring these three genes was used as donor for marker assisted backcross breeding. The segregating progenies in the backcross generations were subjected to marker assisted foreground

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selection using gene linked markers, the selected plants evaluated for agromorphological traits, grain and cooking quality followed by background selection with 58 polymorphic genome wide SSR markers. Recombinant selection using flanking markers near target genes was carried out to reduce linkage drag. Plants homozygous for the genes Pi54+Pi1+Pita, Pi54+Pi1, Pi54+Pi ta, Pi1+Pita were identified in different backcross generations namely, BC2F6, BC3F5 and BC4F4, respectively. results: The RPG recovery ranged in the three and two gene pyramids from 93.6 to 98.6% and improved lines viz., Pusa-1633-4-2, Pusa-1633170-6, Pusa-1633-30-8, Pusa-1633-1623 and Pusa-1633-101-4, expressed resistance reaction to diagnostic isolates from Basmati growing regions. The improved lines possessed excellent grain and cooking quality besides good agronomic performance, at par with check Pusa Basmati 1. Conclusion: Marker assisted backcross breeding was successfully employed for pyramiding three blast resistance genes into popular Basmati rice cultivar Pusa Basmati 1. The deployment of pyramided lines as improved cultivars will help to achieve durable resistance to rice blast in Basmati growing regions of India.

1

R.K. Ellur , S. Pathania , S. 1 1 Gopalakrishnan , P.K. Bhowmick , M. 2 2 3 Nagarajan , K.K. Vinod , U.D. Singh , R. 4 5 1 Rathour , K.V. Prabhu , A. Singh 1

Division of Genetics, Indian Agricultural Research Institute, New Delhi, India 2 Rice Breeding and Genetics Research Centre, Indian Agricultural Research Institute, Aduthurai, India 3 Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India 4 Department of Agricultural Biotechnology, CSKHPKV, Palampur, India 5 Indian Agricultural Reserach Institute, Division of Genetics, New Delhi, India introduction: Pusa Basmati 1121 (PB1121) is a Basmati rice variety with exceptional grain and cooking quality and fetches highest price in the domestic and international markets. It is susceptible to blast disease caused byMagnaporthe grisea, which affects both yield as well as grain quality. Methodology: Marker assisted simultaneous but step wise backcross breeding (MASS-BB) was used to incorporate the blast resistance genes Piz5 and Pi54 from Pusa 1602 and Pusa 1603 respectively, into PB1121 using gene linked markers. Inter-crossing was done at BC3F1 generation to pyramid both the blast resistance genes. In each backcross generation, the plants selected for respective genes using foreground markers were subjected to rigorous phenotypic and background selection to accelerate the recovery of recurrent parent phenome and genome.

P0049 PyraMiDing OF BLaST reSiSTanCe geneS in THe BaSMaTi riCe VarieTy PUSa BaSMaTi 1121 THrOUgH MarKer aSSiSTeD BaCKCrOSS BreeDing

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results: MASS-BB led to the development of NILs Pusa 1716 (PB1121+ Piz5) and Pusa 1717 (PB1121 + Pi54) with RPG recovery of 93.8 % and 94.7% using 58 and 56 STMS markers representing genome wide coverage, respectively. The pyramided lines (PB1121 + Piz5 + Pi54) and NILs exhibited resistance to blast under artificial inoculation with respective diagnostic isolates. The improved lines showed performance on par with PB1121 for agronomic and cooking quality traits. Discussion and conclusion: Marker assisted backcross breeding was successfully employed for pyramiding two blast resistance genes into popular Basmati rice cultivar Pusa Basmati 1121. The pyramided lines will be evaluated under multilocation trials for release as improved cultivar and as an invaluable source for blast resistance in Basmati rice improvement.

the deployment of resistance (R) genes. Use of resistant rice varieties would be the most effective way to control this disease; therefore, mining the resistant genes might be important foundational work in the breeding program. India is the reservoir of rice biodiversity with huge number of traditional landraces. In the present study three well characterized genes, viz.,Pi1, Pi2 (Piz5) and Pi5 that are known to confer broad spectrum resistance to blast were mined from landrace collections of Karnataka state, India, by using PCR (polymerase chain reaction) based molecular markers. The results indicated the presence of Pi-1 gene in 39 landraces, accounting for 45.24% of 84 landraces analysed. Pi-z5and Pi-5 gene were present in 60 and 59 landraces accounting the distribution frequency of 71.43% and 70.24% respectively. Few landraces showed the presence of all the three genes mined in this study and showed blast resistance in different hotspots tested in India and hence deserve the attention as the natural gene pyramids. They serve as important gene pool for future breeding programme for blast resistance.

P0050 MOLeCULar CHaraCTerizaTiOn OF riCe LanDraCeS OF KarnaTaKa, inDia FOr BLaST reSiSTanCe 1

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P. S.K , K. Ingole , K. PU


P0051

1

MarKer aSSiSTeD reCUrrenT SeLeCTiOn(MarS) FOr iMPrOVing BiOTiC STreSS reSiSTanCe OF geneTiC MaLe STeriLe reSTOrer POPULaTiOn

1

Department of Biotechnology, University of Agricultural SciencesDharwad, Dharwad, India Rice blast, caused by Magnaporthe oryzae (Couch & Kohn, 2002) is one of the most serious diseases of rice in all rice growing regions of the world. The disease can be effectively managed by

1

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P. Revathi , S. Janardhan , S. Arun 1 1 Kumar , P. Senguttuvel , K.B. Kempa 1 1 Raju , R.M. Sundaram , A.S. Hari 1 1 Prasad , B.C. Viraktamath

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improving various target traits like plant height, grain yield and fertility restoration etc.

1

hybrid rice section, directorate of rice research, HYDERABAD, India

One of the interesting methods of improving hybrid rice parental line is through genetic male sterility facilitated recurrent selection, which not only broadens the genetic base of parental lines but also helps in pyramiding the desirable genes. Population improvement programme involving male sterility have made little use of molecular markers, even though they could be very helpful in targeted selection of the desirable recombinants. In the present study IR36 genetic male sterile (GMS) restorer population sterile plants were crossed with donors of Xa21 for BLB resistance, Pikh for blast resistance, Bph 20 & 21 for BPH resistance and Gm 4 for gall midge resistance. The F1s plants were validated and confirmed for the presence of resistant genes with the help of molecular markers. The bulked F2 seeds were raised in isolation for random mating and the sterile plants of the population confirmed for the presence of biotic stress resistant genes by MAS were harvested and bulked to constitute for recurrent selection cycle and in the same way recurrent selections were continued upto 4 cycles. In the fourth cycle around five hundred plants were screened for the presence of BLB, blast, BPH, gall midge resistance and Rf genes by MAS. Harvested male fertile plants identified to carry multiple biotic stress resistance genes are being handled by pedigree method of breeding for restorer line development and marker assisted recurrent selection is in progress for

P0052 PyraMiDing OF MaJOr Pi geneS in riCe FOr DUraBLe reSiSTanCe againST DiVerSe BLaST raCeS in nOrTH eaSTern HiLL regiOn OF inDia 1

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M. Rai , G.F. War , M.R.J. Najiar , B. 1 1 Kumari , W. Tyagi 1

School of Crop Improvement, College of PG Studies Central Agricultural University, Shillong, India

rationale: Marker Assisted Backcrossing (MAB) is an effective approach to introgress and pyramid blast resistance genes in rice to achieve durable resistance against rice blast that causes upto 46 percent yield loss in the North Eastern Hill Region (NEHR) of India. Methodology: Six advanced backcross generations (BC3F1/BC2F1) obtained from crossing recipients Shahsarang, CAUR1 (lowland genotypes) and Bhalum 3 (upland rice genotype) with donors IRBL9W (carrying Pi9 gene) and DHMAS (carrying Pi-1, Pita and Pi54 genes) were screened for donor foreground by using linked Simple Sequence Repeat markers (SSR) viz. RM 206 (Pi54), RM 224 (Pi1), RM 247 (Pita) and RM 12705 (Pi9) polymorphic between parents. results: Out of the 428 BC3F1/BC2F1 progenies screened, 103 plants were found to carry at least one

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Japan Kyushu Okinawa Agricultural Research Center, National Agriculture and Food Research Organization, 2421 Suya Koshi Kumamoto 861-1192, Japan 4 Plant Breeding Laboratory Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki Higashi Fukuoka 812-8581, Japan

donor foreground allele. Seventeen backcross plants of Shahsarang and DHMAS were found to have two genes and three progeny plants carried three gene combination. On the basis of marker assisted foreground selection, disease score, and background reconstitution as revealed by SSR markers and DUS characters BC3F1/BC2F1 lines for all the cross combinations were selected for further pyramiding. Also, twenty blast isolates collected from various locations of the NEHR were pathotyped against 17 monogenic differential lines to find out their virulence status with respect to individual resistance genes. Discussion and conclusion: The backcross progenies identified in the current study along with the pathotyping data obtained using lowland blast isolates will be helpful in designing future breeding strategies against blast disease relevant to rice production in lowland ecosystem of NEHR.

3

The brown planthopper (BPH), Nilaparvata lugens StŒl (Homoptera: Delphacidae), is one of the most economically important insect pests of rice throughout Asia. A linkage map is valuable genomic resources and represents a powerful research tool for identifying the genetic basis of important traits. In this study, we developed the first genetic linkage map for BPH. The linkage map was constructed by integrating linkage data from two backcross populations derived from three inbred BPH strains. The consensus map consists of 474 simple sequence repeats, 43 single-nucleotide polymorphisms, and 1 sequence-tagged site, for a total of 518 markers at 472 unique positions in 17 linkage groups. The linkage groups cover 1093.9 cM, with an average distance of 2.3 cM between loci. The sex-linkage group was identified by exploiting X-linked and Yspecific markers. The linkage map and the newly developed markers used to create it (available at http://sogo/.dna.affrc.go.jp/cgibin/sogo.cgi?class=unka) constitute an essential resource and a useful framework for future genetic analyses in BPH such as genetic mapping of biotype-associated genes, which are now undergoing discovery.

P0053 a FirST generaTiOn MiCrOSaTeLLiTe- anD SnP-BaSeD LinKage MaP OF BrOWn PLanTHOPPer 1

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J. Jairin , T. Kobayashi , M. Matsumura , 4 H. Yasui 1

Ubon Ratchathani Rice Research Center, Rice Department, P.O.Box 65 Muang Ubon Ratchathani 34000, Thailand 2 Division of Insect Sciences, National Institute of Agrobiological Sciences, 1-2 O-washi Tsukuba Ibaraki 305-8634,

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for agro-morphological characters during Rabi, 2012-13. Seed multiplication of these introgressed lines is in progress in order to take up yield evaluation trials.

Development of biotic stress resistant MTU 1010 using marker assisted breeding 1

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P0055

D. Ch.V. , M.D. Jamaloddin , G. Swathi , 1 S. Vanisree 1

Institute Of Biotechnology, Institute Of Biotechnology, HYDERABAD, India MTU 1010, a mega rice variety has been aimed to improve major resistance genes of BB (xa13 and Xa21), blast (Pi54, Pi1 and Pi2) and gall midge (Gm4) through marker assisted backcross breeding. Functional (xa13promo forxa13, Pi54MAS for Pi54 and STS marker pTA248 for Xa21) as well as closely linked (RM547 for Gm4, RM224 for Pi1 and RM527 for Pi2) markers were used for foreground selection. 82 and 83 markers were found to be polymorphic between MTU 1010 vs. GPP2 and MTU 1010 vs. NLR 145, respectively and were used for background selection. GPP2 (xa13, Xa21 and Gm4) and NLR 145 (Pi54, Pi1 and Pi2) were used as donor parents for making two crosses (MTU 1010 x GPP2) and MTU 1010 x NLR145) during Rabi, 2009-10. In BC2F1 generation, a triple positive plant, Xa13xa13 Xa21xa21 Gm4gm4 with 91% MTU 1010 genome, while another triple positive plantPi54pi54 Pi1pi1 Pi2pi2 with 94% MTU 1010 genome were identified. In BC2F2 generation, six and nine plants were found with gene combinations of Xa13Xa13 Xa21Xa21 Gm4Gm4 and Pi54Pi54 Pi1Pi1 Pi2Pi2, respectively were found resistant to BB and blast. These resistant families were evaluated

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The development of transgenic rice lines with potential resistant to yellow stem borer and their impact on biodiversity of non-target organisms in confined field trial 1

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A. Estiati , P. Lisdiyanti , S. Nugroho , Y. 2 3 Rahayuningsih , N. Usyati , T. 4 4 Chrismada , G.P. Yoga 1

Research Center for Biotechnology, Indonesian Institute of Sciences, Indonesia 2 Research Center for Biology, Indonesian Institute of Sciences, Indonesia 3 Indonesia Center for Rice Research, Indonesia, 4 Research Center for Limnology, Indonesian Institute of Sciences, Indonesia Yellow stem borer (Scirpophaga incertulas Walker) is a destructive pest in rice field and become a serious problem in Indonesia. The symptom at vegetative stage called deadhearts while at reproductive stage called whitehead. In Indonesia, the average damage by yellow stem borer in the last 10 years was 84.952 ha. Every 1% whitehead symptoms it is correlated with 1% rice production losses. Unfortunately, until now no rice resistant plants have been found, therefore transgenic techniques offer a useful alternative, besides this


approach is environmental friendly. We have developed Javanica rice lines cv. Rojolele overexpressing the Bt toxin cry1B gene controlled by inducible gene promoter maize proteinase inhibitor (mpi). The overexpresion has been confirmed both by Western blot and Real-Time PCR. Bioassay performed in the biosafety containment indicated increase level of resistance against yellow stem borer. Currently, confined field trial to study the impact of the event on non-target organisms, i.e. predator, collembola, soil insects, soil microbes, plankton and macroavertebrates are underway. The progress of the trial will be presented.

genetic backgrounds form a bottleneck in translating this into variety development. Here we describe using a very large mutagenized population of indica rice, combined with TILLING (Targeted Induced Local Lesions IN the Genome), to harness rice functional genomics into product development. We are identifying new allelic variation in multiple genes, all in the same genetic background, for traits such as yield, abiotic stress tolerance, disease resistance, grain quality, and hybrid development. Methodology Using a combination of EMS and ENU chemical mutagens, we created a very large indica population (variety IR64). DNA was extracted from M2 generation plants and the seeds and corresponding DNA from each plant bar coded and stored as a long-term 'library.' The library is screened for mutation using PCR primers designed to amplify specific single genes and SNPs in those genes identified using an endonuclease-based mismatch detection system. results and Discussion We achieved a mutation frequency of 1 in 250 KB and created library of 10,000 indica plants, resulting in an average of 50 mutations per 1 KB. We demonstrate that this frequency generates allelic series of phenotypes, increasing the likelihood of finding commercial traits. We report on novel alleles identified for several traits, including increasing yield by targeting changes in plant architecture, grain quality, and male sterility for hybrid production in our commercial pipeline.

KeyWords: confined field trial, cry1B gene, GM rice, non-target organisms, yellow stem borer P0056 HarneSSing FUnCTiOnaL genOMiCS & neW aLLeLiC DiVerSiTy in PrODUCT DeVeLOPMenT 1

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V. Knauf , D. Facciotti , C. McGuire , D. 2 Loeffler 1

Research, Arcadia Biosciences, Seattle, USA 2 Research, Arcadia Biosciences, Davis, USA introduction Despite large and diverse functional genomics research on rice, the complexity of mining data, limitations of screening some traits, and difficulty accessing rare mutations in diverse

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P0057

(PIC) lied between 0.455 to 0.934. Pairwise genetic dissimilarity co-efficient showed the lowest genetic dissimilarity in the high yielding Aus rice genotypes and the highest genetic dissimilarity was found in the local Aus landraces. The results of this study would provide valuable information for future breeding program to incorporate drought resistance and early maturity genes into the existing high yielding rice by using these local Aus landraces and thus the total rice production of Bangladesh can be increased.

geneTiC DiVerSiTy OF SOMe BangLaDeSHi aUS riCe genOTyPeS 1

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1

M.E. Hoque , M.M. Islam , S. Sultana , J. 1 1 Ferdous , M.S. Ali 1

Biotechnology Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh

Bangladesh has a long history of rice cultivation having three major ricegrowing seasons, Aus, Aman and Boro resulting in a diverse array of local Bangladeshi rice cultivars. Among them Aus rice is a valuable genetic resource due to its drought tolerance and early maturity. Although some have been incorporated into modern breeding programs, the vast majority of these landraces remain untouched. To better understand this rich source of genetic diversity we have evaluated 94 Bangladeshi Aus rice genotypes, including 6 high yielding varieties and 88 local landraces both at morphological and molecular level. Among the 13 morphological characters evaluated, length-breath (LB) ratio of grain had the highest contribution followed by days to flowering, days to maturity, grain per plant and yield per plant,respectively. Genotypes were further screened with 91 microsatellite markers for molecular diversity analysis. Among the 91 amplified products, 56% have polymorphic bands giving 195 alleles. The number of alleles per locus ranged from 4 to 27, whereas average allele number was 9.76 per locus. The Polymorphism Information Contents

P0058 geneTiC DiVergenCe anaLySiS USing yieLD anD yieLD reLaTeD TraiTS in riCe (Oryza SaTiVa L.) 1

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N.P.S. De Silva , B.P.S. Malik , K.R. 3 Battan 1

Department of Agriculture, Regional Rice Research and Development Centre, Kalutara, Sri Lanka 2 Department of Plant Breeding, CCS Haryana Agricultural University, Hisar, India 3 Department of Plant Breeding, Rice Research Station CCS Haryana Agricultural University, Hisar, India Genetic divergence for grain yield and yield related characteristics in 68 rice genotypes was studied at Rice Research Station, Kaul, Haryana, India during Kharif 2007-08. The analysis of variance revealed significant differences among the genotypes for all the 2 characteristics studied. D statistic was used as a tool for estimating genetic

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P0059

divergence. (Mahalanobis, 1936; Rao,1952). Based on the genetic distance, 68 genotypes were grouped into nine clusters. It indicated existence of wide genetic divergence among genotypes. The characteristics like grain yield/plant, number of grains/panicle, plant height and kernel length/breadth (L/B) ratio contributed the maximum towards genetic diversity. The distribution of genotypes from different eco-regions into various clusters was at random indicating that geographic diversity and genetic diversity were not related. Maximum intra-cluster distance was observed in cluster VII comprising of four rice genotypes. Based on cluster mean, the genotypes in cluster VII had the highest mean performance for number of effective tillers/ plant, panicle length, hulling %, kernel length and kernel L/B ratio. As far as the cluster means were concerned different clusters have higher mean values for different traits indicating that none of the cluster contained genotypes with all the desirable characters. The maximum inter-cluster distance was recorded between cluster V and VII indicating that they were the most divergent clusters. These observations suggest that intercrossing of genotypes from different clusters showing good mean performances may be help in obtaining high yield with better grain quality. Key words: Genetic divergence, grain 2 yield, D analysis, cluster, rice

aLLeLiC DiVerSiTy BaSeD On MiCrOSaTeLLiTe MarKerS LinKeD TO SHeaTH BLigHT reSiSTanCe in riCe (Oryza SaTiVa L.) 1

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M.K. Hossain , M.A.R. Bhuiyan , R. 1 Wickneswari 1

School of Environmental and Natural Resource Sciences Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia Rice sheath blight causes enormous yield loss in rice growing areas all over the world. The yield loss can be reach up to 50% under favorable disease environment. No complete immune source has been identified against the disease in rice germplasm yet. However, there are few major as well as minor quantitative trait loci (QTLs) have been detected for sheath blight resistance. A total of 24 rice genotypes including six designated as moderately resistant to sheath blight (Teqing, Tetep, Jasmine85, Pecos, Azucena and Taducan) four Oryza rufipogon accessions and the remaining of advanced breeding lines and cultivars were genotyped with 15 SSR markers linked to sheath blight resistance to evaluate the predisposition of sheath blight marker alleles for genetic improvement. Fifty nine alleles were detected across the 24 rice genotypes with 2 to 7 alleles per locus. There was no clear distinction of genotypes between the moderately resistant cultivars and the other rice genotypes evaluated in this study with the exception for SSR markers RM245 (qSB-9-2), RM434 (qShB9-1) and

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RM224 (qSBR11-1). UPGMA clustering analysis based on Nei's genetic distance showed five distinct clusters with the moderately resistant cultivars dispersed in three clusters providing sufficient choices for introgression of major QTLs into susceptible rice genotypes.

alleles were amplified for all these rice cultivars using 15 SSR markers. PIC values varied from 0.00 (RM 307) to 0.87 (RM 226). Similarity distance varied from 0.00 to 1.00. Dendrogram generated by UPGMA showed three distinct clusters with Pusu and Laila significantly diverted from the other eight rice cultivars. Discussions and Conclusions: The lowest value of similarity distance as that for Laila and Adan indicated that both could be used in breedings program. High similarity distance value might be caused by different name given by the local farmers when originally they came from same source. Dendrogram generated by UPGMA concluded that Laila and Pusu were more genetically related to each other compare to the other rice cultivars. Keywords: genetic variability, rice cultivars, SSR markers

Keywords: Oryza sativa, QTLs for sheath blight resistance, SSR markers, allelic diversity, moderate resistance. P0060 aSSeSSMenT OF HOW geneTiCaLLy reLaTeD 10 riCe CULTiVarS CULTiVaTeD in BrUnei USing MiCrOSaTeLLiTe MarKerS 1

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N. Ishak , Z. Sulaiman , K. Tennakoon

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Biology, Universiti Brunei Darussalam, Bandar Seri Begawan, Brunei

P0061

Introduction: Limited range for genetic variability and lack of knowledge about genetic info for rice cultivars might probably cause for the low production in rice. Conserved use of genetic variability and diversity may help in improving rice cultivars productivity especially using breeding program. Methodology: In order to study the genetic variation of these cultivars, molecular markers are thought to be a strong tool to do so. Genetic variations of ten different rice cultivars that are cultivated in Brunei were studied using 15 different markers (SSR). Results: Three SSR markers, RM 226, 276 and 333, showed polymorphic alleles while the other 12 were most probably monomorphic. A total of 76

genOMe WiDe aSSOCiaTiOn STUDy (gWaS) FOr agrOnOMiC anD yieLD-COMPOnenT TraiTS USing a SeT OF eLiTe irrigaTeD BreeDing LineS 1

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H. Begum , V. Lopena , V. Bartolome , P. 1 1 2 Virk , G. Gregorio , J. Spindel , S. 2 1 McCouch , B. Collard 1

PBGB, International Rice Research Institute, Los Banos, Philippines 2 Plant breeding and genetics, Cornell University, New York, USA Genome wide association studies (GWAS) are used to identify quantitative

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trait loci (QTLs) for traits of interest. A collection of 440 diverse O. sativa elite lines from the irrigated breeding program at IRRI formed the panel used in this study. These elite breeding lines were derived from crosses between indica x indica, indica x temperate japonica, indica x tropical japonica, and indica x basmati. All lines were phenotypically evaluated during the dry and wet seasons in Los Ba–os, Philippines during 2012. Traits included heading date, plant height, culm length, panicle number and length, spikelets per panicle and flag leaf characteristics. Linear mixed models were used to predict mean trait values and determine variance components using Genstat. There was significant genetic variation for all traits measured. Heading date was a striking example of phenotypic variation, ranging from 70 to 110 days after sowing. A total of 78,286 SNPs, generated using genotyping-bysequencing (GBS) at Cornell University, were used as the basis for associating genotype with phenotype. Principle components analysis (PCA) was used to determine and control for population structure. Preliminary association mapping results will be presented in this paper.

1

Applied Bioscience, Konkuk University, Seoul, Korea Although a great deal of rice proteomic research has been conducted, there are relatively few studies specifically addressing the rice pollen proteome. Rice pollen is sensitive to temperature changes thus low and high temperature cause sterility of panicles. Here, we performed comparative shotgun proteomic analysis of rice pollen development to construct an in-depth proteome reference map, monitoring the expression patterns of the identified proteins, and to detect proteins that are expressed differentially during pollen development. Anther were harvested in 20 days before flowering, 10 days before flowering, and flowering day. The protein expression patterns were revealed by a quantitative shotgun proteoemic analysis. Extracted proteins were separated in 1D-SDS-PAGE then the gel was sliced into seven. Chopped gels were ingeldigested. Peptides were subjected to mass spectrometry (Q-Exactive) ms/ms spectra were analyzed through Proteome Discoverer. By merging all of the identified proteins in the seven sliced gel samples, total anther proteome reference map was constructed. The total spectra counts assigned to a protein were used for quantification. Proteins expression patterns were investigated by comparing the quantity of protein. The proteins expression patterns were identified in this study were clustered. Clustering analysis and Genome Ontology category enrichment analysis revealed that proteins involved in the metabolic process were enriched through all stages of development. With

P0062 SHOTgUn PrOTeOMiCS aPPrOaCH TO MOniTOr PrOTeOMe eXPreSSiOn PaTTernS OF riCe anTHerS DUring DeVeLOPMenT 1

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H. Kim , J. Cho , J. Lee , J. Lee

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African recipient varieties lacking PSTOL1 need to be identified. Gene-specific markers were used to survey the presence of PSTOL1 and other genes of the Pup1 locus in African mega varieties, NERICA varieties and their O. glaberrima parents. Here, we report the presence of a novel PSTOL1 allele in NERICAs that was inherited from the O. glaberrima parent CG14. This allele is present in most glaberrima accessions evaluated, and showed 35 base pair substitutions when aligned to the Kasalath allele but a fully conserved kinase domain. In silico and marker analysis indicated that many other genes of the Kasalath Pup1 locus were missing in the O. glaberrima genome, including OsPupK20 which was shown to be downstream of OsPSTOL1. We have developed several allele-specific markers for use in MAS to transfer the entire Pup1 locus from Kasalath to African megavarieties including NERICAs.

a label free shotgun proteomic approach, this is the report conducting comprehensive identification of rice anther proteins P0063 SearCHing FOr TOLeranCe TO P DeFiCienCy in aFriCan riCe (O. gLaBerriMa). a SUrVey FOr THe PreSenCe OF P STarVaTiOn TOLeranCe gene (PSTOL1). 1

2

J. Pariasca-Tanaka , K.N. Drame , J.H. 3 3 1 Chin , S. Heuer , M. Wissuwa 1

Crop Livestock & Environment Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Japan 2 Genetic Diversity and Improvement Program, AfricaRice, Cotonou, Benin 3 Plant Breeding Genetics and Biotechnology, IRRI, Los Banos, Philippines The deficiency of phosphorus (P) in soil is a major problem in Sub-Saharan Africa due to general nutrient depletion and the presence of P-fixing soils. The development of rice cultivars with enhanced P efficiency would represent a sustainable strategy to improve the livelihood of resource-poor farmers. Recently the Pup1 locus, a major QTL for tolerance to P deficiency in soil was successfully narrowed-down to a singlecandidate gene, the protein kinaseOsPSTOL1 (P starvation toleranc e). Our target is to improve the tolerance to P deficiency of African mega varieties through marker assisted introgression of PSTOL1 from donor variety ÔKasalathÕ, and as a first step, suitable

P0064 raiSing inTrOgreSSiOn LineS FrOM aa genOMe SPeCieS OF genUS Oryza FOr gene DiSCOVery anD geneTiC iMPrOVeMenT 1

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J. Zhou , P. Xu , J. Li , Y. Zhang , X. 1 1 1 2 Deng , F. Hu , W. Deng , Z. Wu , J. 2 2 2 1 Zhao , Y. Yu , Y. Yang , D. Tao 1

Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China 2 Life-sciences school, Yunnan University, Kunming, China

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P0065

Introgression lines (ILs) are necessary tool to identify traits of yield, resistance to or tolerance for stresses, and detect favorable genes of wild rice. Seventythree accessions of wild rice species with AA-genome, O. bathii, O. rufipogon, O. nivara, O. glumaepatula, O. meridionalis, O. longistaminata, and 160 upland rice varieties or landraces of O. sativa, 59 strains of O. glaberrima as donors, temperature japonica varieties, Dianjingyou 1 and Yundao 1 as recurrent parents (for O. longistaminata, indica cultivar RD23 from Thailand was used as recurrent) were used to cross and backcross. Progenies were self-fertilized from BC2F1 and BC3F1, and screened from BC2F3 and BC3F3 based on phenotype compared with the recurrent parents. At last, 26,763 introgressed BC2 and BC3 families were raised for screening purpose. And 3,273 introgression lines (ILs) in Yundao 1 background, 3,050 ILs in Dianjingyou 1 background, 265 ILs in RD23 background with 19 traits responsible for yield and other traits were raised. Phenotypic variation of the ILs was abundant, and the variation of grain shape was easily to be found in the ILs, which will be useful for gene discovery, breeding utilization, and genetic study of these traits. acknowledgements This research is supported by grants from The National Natural Science Foundation of China (31160275, 31201196, U1036605), Ministry of Agriculture (2009ZX08009107B),PeopleÕs Republic of China?

QTL MaPPing OF PaniCLe TraiTS USing inTrOgreSSiOn LineS FrOM aa-genOMe SPeCieS 1

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1

P. Xu , Y. Yu , J. Zhou , J. Li , Y. 1 1 Zhang , D. Tao 1

Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, China 2 Life-sciences school, Yunnan University, Kunming, China Introgression lines (ILs) are useful tools in identifying the important agronomic traits, and detecting favorable genes from wild rice pecies. In order to mine favorable alleles from the relatives or wild species, 6323 BC2F7 and BC3F6 introgression lines (ILs) with 13 different traits introgresed from 7 AA genome species of Oryza genus were raised in temperature japonica varieties, Dianjingyou 1 and Yundao 1 backgrounds. When 1403 ILs with different traits in Dianjingyou 1 background were genotyped by 168 well distributed SSR markers, 269 QTLs were detected, including grain shape, grain weight, panicle type, plant height, seed color, pericarp color and other agronomic traits. To confirm panicle type QTL detected, an F2 segregation population was raised (O. from BC3F7 IL-2009H3B1378 nivara)/Dianjingyou 1. The results showed that spreading panicle trait was controlled by a single dominant geneSpr3(t) mapped on chromosome 4 flanked by SSR markers RM349 and RM3466. At the same time, a QTL controlling panicle number between

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RM17000 and RM3367 on chromosome 4 and a cluster of QTLs including spikelet number, spikelet density, panicle length on chromosome 7 near marker RM3394 were detected. acknowledgements This research is supported by grants from The National Natural Science Foundation of China (31160275, 31201196, U1036605), Ministry of Agriculture (2009ZX08009107B),Ministry of Science and Technology (U1036605).

the SNP markers was conducted in GAMMA Laboratory of IRRI. The results showed that the gene diversity (0.2398) and PIC (0.1939) value was relatively low. Phylogeny analysis clustered the genotypes into nine groups at genetic distance of 0.1. Among them, some varieties were stood alone, i.e. Dodokan, Silugonggo (new release very early maturing); PB5, Diah Suci, IR42 (old released late maturing); and Fatmawati (new released NPT). Cisadane and Pelita I 1 (old released late maturing) were grouped together. Sarinah and Inpari 6 Jete (new released early maturing) were grouped together. All the rest were grouped together and were new released variety, i.e. Ciherang, IR64, Widas, Inpari 10, Inpari Sidenuk, Cibogo, and Mekongga. Ciherang, the most popular variety, differed only in one loci with IR64. It was derived from four time backcrossing of IR18349-53-1-3-13/3*IR19661-131-3-1-3 to IR64. The genetic variability of the released variety in Indonesia is relatively low. Recent effort to more utilize local varieties, introduce genotypes, trangenics, and mutation hopefully would increase the genetic variability of the forthcoming varieties.

P0066 aSSeSSMenT OF geneTiC VariaBiLiTy OF 17 OLD anD neW reLeaSeD LOWLanD riCe VarieTieS in inDOneSia USing 384 SnP MarKerS 1

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U. Susanto , N.A. Rohmah , P. Lestari , 1 I.M.J. Mejaya 1

Plant Breeding, ICRR of IAARD, Subang, Indonesia 2 Germ Plasm Conservation, ICABIOGRAD of IAARD, Bogor, Indonesia Genetic variability is important to increase itÕs buffering capacity to respond to the environmental dynamics. This study was aimed to see the diversity among old and new released lowland rice varieties in Indonesia. The experiment was conducted during 2011 using seventeen genotypes consisted of 6 old and 11 new released varieties (after 2000 AD). DNA extraction was done in ICABIOGRAD of IAARD using CTAB method and running

P0067 SOrgHUM MUTageneSiS: UnVeiLing geneS OF C4 PHOTOSynTHeSiS 1

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R. Garcia , G. Rizal , K. Acebron , N.A. 1 1 1 Larazo , M. Alcasid , N. Elayda , J. 1 1 1 Salonga , A. Mabilangan , M.J. Dionora , 1 W.P. Quick

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mutant lines and then transgenically tested for efficacy in rice.

1

C4 Rice Center, International Rice Research Institute, Los Banos, Philippines

P0068 DEVELOPMENT OF “KEY MARKER” COnCePT FOr PreDiCTiOn anD VaLiDaTiOn OF HeTerOSiS USing MiCrOSaTeLLiTe MarKerS in ParenTaL LineS OF riCe (Oryza SaTiVa L).

Sorghum bicolor is a model C4 crop with high yield potential even in adverse grow ing conditions. C4 crops have higher nitrogen and waterand use efficiency than C3 crops the genes controlling many aspects of C4 photosynthesis are still unknown. This study aims to screen the mutant sorghum population for loss-ofC4 function traits, to determine the trait inheritance pattern on back-crossed populations and to conduct gene sequencing analyses. Leaf anatomical analysis of mutants showed lower vein density compared to the wild-type (WT). One of the causes of changes in vein spacing was an increase in the number of interveinal mesophyll cells (i.e. 4-5 cells) which are distinct characteristics of anatomy. Gas exchange C3 leaf measurements of low-vein-density (LVD) mutants revealed a significant decrease in carboxylation efficiency (CE) and maximum photosynthetic (Pmax) rate as compared to WT. These changes in CE and Pmax suggest a malfunction in the photosynthesis of LVD mutants. The F2 back-crossed progenies followed a Mendelian segregation pattern of 1:3: LVD: WT VD ratio indicating LVD phenotype is controlled by a single recessive trait. Gene sequencing analysis of the back-crossed population show 5 candidate genes with nucleotide base substitutions that lead to nonsynonymous amino acid changes. These are currently being tested for alterations in expression in the

1

1

S. SONI , R.M. SUNDARAM , V.K. 2 3 Yadav , V.P. Bhadana 1

Biotechnology, Directorate of Rice Research, HYDERABAD, India 2 Genetics and Plant Breeding, C.S.Azad Univ. of Agril. & Tech. Kanpur, Kanpur, India 3 Genetics and Plant Breeding, Directorate of Rice Research, HYDERABAD, India Heterosis has been widely exploited in a number of crops, and contributed significantly to agricultural production worldwide by increasing yield broadening the adaptability of hybrid varieties. Therefore, an approach to enhance the efficiency of hybrid breeding especially in rice parental lines by predicting and validating the hybrid performance to formulate the Òkey markerÓ concept was undertaken with fifteen genotypes comprising 10 japonica as lines and 3indica as testers along with two reference varieties Jyothi (indica) and Nipponbare (Japonica) at Biotechnology Laboratory, Crop Improvement Section, DRR, Rajendranagar, Hyderabad (AP) during 2013. Among 43 studied HRM markers, 40 generated 131 bands with an

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Univ. of Agril. & Tech. Kanpur U.P., Kanpur, India 3 Genetics and plant Breeding, Directorate of Rice Research, HYDERABAD, India

average of 3.28 alleles per locus indicated that all SSR markers were polymorphic. Significant and positive correlation of 0.350* was observed between the number of alleles and PIC values with average PIC value of 0.542 indicated presence of substantial genetic diversity. The primer HRM25796 displayed the highest PIC value of 0.775 considered as highly informative marker.The standard heterosis ranged from -78.11 to 30.61 over sarjoo52 and -80.73 to 14.95 over NDR-359 for TJ * 3950 × PB-1, and TJ * 11010 × PB-1having CMP value of 0.744 and 0.658 respectively, indicated strong association between the marker polymorphism and heterosis but not in the direction. The correlation between CMP and heterosis was low over three standard varieties indicating, markers selected for the present study may not be sufficient for such correlation analyses and formulating highly informative Òkey markerÓ concept. Therefore, need to include more markers and more hybrid combinations.

Under Variety Protection Act 2001, India need to verify the identity of a variety to establish distinctness, has become very critical for variety registration. DNA markers offers fast and accurate identification and purity for variety. Hence 40 world mini core collections for genetic relatedness and uniqueness along with two reference varieties Jyothi (indica) and Nipponbare (Japonica) were carried out at Biotechnology Laboratory, Crop Improvement Section, DRR, Rajendranagar, Hyderabad (AP) during 2013. Out of 43 studied HRM markers, 35 generated 118 bands with an average of 3.37 alleles per locus, the primer HRM 28110 displayed the highest PIC value of 0.778 revealed that HRM28110 might be the best marker for identification and similarity estimation among world mini core rice collections. Dendrogram was divided into two sub-clusters i.e. cluster I A1 and cluster I A2. Highest 88% similarity was observed between PR33319-9-1-1-5-35-4-1 and PR-114 genotypes. Cluster second consisted of two genotypes TJ39050 and Nipponber was distinct from the remaining genotypes and most suited to initiate the heterosis breeding programme. Principal Component Analysis resulted if TJ 50836 and TJ 35724 having similarity of 54% are crossed with PR33319-9-1-1-5-3-5-4-1 and PR-114 there be greater chance of finding high heterotic crosses. HRM 28110 amplified a unique allele 900 bp

P0069 genOTyPing OF WOrLD Mini COre COLLeCTiOnS, THeir UniQUe iDenTiFiCaTiOn anD geneTiC SiMiLariTy USing MiCrOSaTeLLiTe MarKerS in riCe (Oryza SaTiVa L.). 1

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S. SONI , R.M. SUNDARAM , V.K. 2 3 Yadav , V.P. Bhadana 1

Biotechnology, Directorate of Rice Research, HYDERABAD, India 2 Genetics and plant Breeding, C.S.Azad

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in BARKE 3004, HRM 26369 (700bp), HRM 17600 (500bp), HRM26329 (190bp), HRM 26213 (800bp), HRM 20196 (600bp) amplified four alleles and HRM 24542 (150bp), HRM16006 (600bp) amplified three alleles, produced unique amplification pattern in the IR80859-561-2-2, TJ-71511, TJ35724 TJ-25892 and Vaishakh respectively for easy identification. P0070 aLLeLe LaDDer FOr PHiLiPPine riCe VarieTaL iDenTiFiCaTiOn 1

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V. Dalusong , L. Santos , L. Perez 1

Plant Breeding and Biotechnology Division, Philippine Rice Research Institute, Science City of Muñoz, Philippines 2 Crime Laboratory, Philippine National Police, Quezon City, Philippines 3 Genetic Resources Division, Philippine Rice Research Institute, Science City of Muñoz, Philippines Sequence Tandem Repeats (STR) allelic ladders are widely used in forensic identification for humans and in some animals and crop species. In rice, the use of allelic ladder for varietal identification is being developed. A survey of alleles was conducted on 186 Philippine rice varieties using the 53 selected STR markers previously evaluated for important characteristics including stability, sensitivity and species specificity. STR fragments of different sizes were selected, pooled and verified by sequence characterization for each STR markers.


Allele ladder in each of the selected rice STR markers were successfully constructed using monoplex and multiplex PCR strategy. Each ladder consists initially of 3-5 alleles and was named according to the number of core repeats it represents. Sequence data of each allele detected in 10 STR markers were already verified. Allele alignment of 186 rice varieties using the ladder revealed the presence of other allele not distinguishable by alignment without ladder. New alleles identified will be incorporated into a standard allelic ladder. The constructed allelic ladders have DNA fragments of known length and sequence and are efficient in distinguishing bands with even 2 bp differences thus, assignment of allele scores is more accurate than with the use of size markers alone. The correct assignments of allele scores are very important in establishing the genotype profile database of Philippine rice varieties. From this study, the use of allele ladder provides a simple, reliable and accurate method of allele scoring for rice varietal identification using STR.

P0071 CUrrenT DiVerSiTy OF ParenTaL geneTiC STOCKS USeD in riCe BreeDing in THe PHiLiPPineS 1

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L.M. Perez , M.I.C. Calayugan , T.E. 2 2 Mananghaya , J.A. Orcino , T.F. 2 Padolina 1

Genetic Resources Division, Philippine Rice Research Institute, Science City of Muñoz, Philippines

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2

Plant Breeding and Biotechnology Division, Philippine Rice Research Institute, Science City of Muñoz, Philippines Genetic variation is the foundation of breeding and the future of agricultural production and food security. The extent of diversity of parental genetic stocks used in current rice breeding programs in the Philippines was investigated in terms of yield enhancing and stabilizing traits and genomic DNA polymorphisms. Total of 434 rice germplasm in 12 discrete breeding groups including released rice varieties, elite breeding lines, green super rice, introductions/traditional rice, blast resistance, bacterial blight/rice tungro virus resistance, brown plant hopper resistance, acid sulphate tolerance, drought/submergence and salinity tolerance, cold tolerance, micronutrient and special purpose rice, Tropical Japonica varieties, and miscellaneous rice varieties were used. Phenotypic variation based on Shannon-Weaver Diversity Index (HÕ) was evaluated on 12 morphological and yield component traits in 2013DS. Percent fertility and 1000 grain weight ranged from 45.2 to 96.3% and 14.9 to 36.7 g, respectively, indicating wide variation and good genetic background of parental germplasm for enhancing yield and productivity in improved varieties. Mean harvest index of the population was 0.4 while plant height ranged from 70-161 cm. Genetic diversity per trait (HÕ) was high (0.73 to 0.86) in all parameters except maturity and culm diameter with low (HÕ = 0.15) and medium (HÕ = 0.35) diversity indices. Molecular genetic variation is being investigated using

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selected microsatellite loci and functional markers associated with resistance to biotic and abiotic stresses and grain quality. The results of this study will explain the current status of genetic variation existing in parental breeding materials of rice in the Philippines. P0072 eVaLUaTiOn OF geneTiC DiVerSiTy aMOng riCe VarieTieS in CaMBODia 1

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2

C. Orn , R. Ishikawa , S. Sakhan , M. 2 1 Ouk , T. Ishii 1

Graduate School of Agriculture Science, Kobe University, Kobe, Japan 2 Plant Breeding Department, Cambodian Agricultural Research and Development Institute, Phnom Penh, Cambodia Cambodia has a wealth of rice varieties that fit into different ecosystems. Although molecular markers have contributed to the success of genome researches, little study on their genetic diversity has been done so far. Here we examined the genetic diversity among 336 Cambodian rice varieties which consist of four groups of local varieties (floating rice, rice in dry season, common non-glutinous rice and glutinous rice groups that were collected in 1950s) and two of modern varieties (domestic and foreign lines currently cultivated). Their total DNA samples were analyzed with 12 SSR markers. In total, 117 electromorph bands were detected and they were treated as


alleles at the molecular loci. Among six rice groups, the foreign imported lines of modern varieties showed a higher average gene diversity value of 0.63 than other Cambodian originated groups (value ranging from 0.40 to 0.57). This higher genetic variation may be coursed by the different origins of the imported lines. The overall diversity value of Cambodian local varieties was 0.55, whereas modern domestic lines gave 0.46. Through the time passed with different histories from 1950s to present, the genetic diversity found to be decreased. This result indicates that modern plant breeding/pure line selection and varieties abandoning causes the reduction of genetic variation. In contrast, the overall gene diversity (0.61) in modern varieties including domestic and foreign lines was higher than that in local varieties suggesting that wide genetic variation of the current rice cultivars in Cambodian is maintained by the introduction of foreign lines.

diversity to accelerate gene discovery and promote precision breeding. Central to this project is the development of a panel of genetic resources that represents the range of diversity in rice germplasm. Initially, the panel was designed to cover ~10% of the total ~117,000 accessions of the International Rice Genebank (IRG). A semi-stratified selection scheme was adopted taking into consideration factors including genome type and species, eco-cultural type and varietal grouping, source country, usage determined by tracing pedigrees in IRIS, and even coverage of name space taking into account potential duplicates (within a country). The resulting panel, dubbed as at the Ò12K coreÓ was complemented by inclusion of nominations from PBGB (IRRI), CIRAD, and CIAT. Genetic stocks were generated from the panel by single seed descent purification followed by seed multiplication under field conditions. The panel was further extended by activities under other projects so that now it comprises approximately 15,000 entries. Subsets of the 15K core have been subjected to genotyping including SSR analysis of a composite selection of about 2,700 accessions, highresolution genotyping of 2,000 accessions with 700,000 SNPs (http://www.ricesnp.org), and re-sequencing of 3,000 accessions using next-generation sequencing (NGS) technology. Subset panels are being phenotyped by a global consortium of partners for impact traits specifically those affected by climate change. Genome-wide association analyses will reveal key SNPs and haplotypes associated with phenotypes. Alleles and their donor accessions can then be

P0073 BUiLDing THe 12K COre FOr THe riCe geneTiC DiVerSiTy PLaTFOrM 1

1

M.E.B. Naredo , S.M.Q. Mercado , F.C. 1 1 1 De Guzman , R.A. Reaño , G.S. Capilit , 1 1 N.R. Sackville Hamilton , K.L. McNally 1

T.T. Chang Genetic Resources Center, International Rice Research Institute, Los Banos, Philippines The Rice Genetic Diversity Platform envisions the efficient use of rice

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targeted to marker assisted breeding programs.

ratio in Brgy. Umiray, Dinggalan, Aurora. Initial results on yield showed NSIC Rc214, PSB Rc82 and NSIC Rc216 as potential combination for cultivar mixture approach. Traditional varieties were selected based on aroma, pigmented pericarp, yield, and DNA polymorphism. Molecular analysis showed Galo, Kasturi, Agsuhay and Ekbi were aromatic rice cultivars. DNA polymorphism using 42 SSRs generated 191 alleles with 4.55 (mean) alleles per locus. Average genetic diversity index was 0.5052 indicating moderately high genetic variation among the varieties. We selected 6 varieties: Galo, Kasturi, Agsuhay, Ekbi, Balatinaw and Palawan for evaluation as cultivar mixtures in Brgy Caragsakan, Dinggalan, Aurora. The potential of use of cultivar mixtures will give options to farmers to increase yield in non-irrigated rice areas in the Philippines.

P0074 aSSeSSMenT OF geneTiC VariaTiOn anD DeVeLOPMenT OF CULTiVar MiXTUreS FOr rainFeD anD UPLanD riCe eCOSySTeM 1

1

L.M. Perez , R.M.F. Cabanting , V.G. 2 2 Dalusong , I.G. Pacada 1

Genetic Resources Division, Philippine Rice Research Institute, Science City of Muñoz, Philippines 2 Plant Breeding and Biotechnology Division, Philippine Rice Research Institute, Science City of Muñoz, Philippines Varietal mixtures have been used as epidemic control strategy for sustainable productivity in agriculture. Rice yields are at risk and often confronted with impacts of environmental variations particularly in rainfed lowland and upland ecosystem. With the largely monoculture rice cultivation in the Philippines, this study attempts to investigate the potential of cultivar mixtures for yield stability of rice in rainfed lowland and upland areas. We used 20 improved varieties and 39 traditional cultivars to develop cultivar mixtures for rainfed lowland and upland, respectively. Based on morphological parameters and genetic diversity of the improved varieties based on 105 SSRs, 4 varieties were selected, PSB Rc82, NSIC Rc214, NSIC Rc216, and NSIC Rc222. These were further evaluated in 42 combinations following 1:1 and 1:1:1

P0075 riCe SHeaTH BLigHT: iDenTiFiCaTiOn OF QUanTiTaTiVe reSiSTanCe THrOUgH aSSOCiaTiOn geneTiCS 1

2

E.G. Oreiro , G. BELIGAN , J. 2 3 AGUIRRE , S. SRINIVASACHARY , S. 4 4 3 SAVARY , L. WILLOCQUET , R. OLIVA 1

Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Banos, Philippines 2 Crop Protection, BASF Philippines Inc., Bay, Philippines 3 Plant Breeding Genetics and Biotechnology, International Rice

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Research Institute, Los Banos, Philippines 4 AGIR, UMR INRA-INP-ENSAT AGIR, TOLOSAN, France

P0076

Rice sheath blight (ShB), caused by Rhizoctonia solani KŸhn [teleomorph: Thanatephorus cucumeris (Frank) Donk] has been an economically important disease of rice worldwide causing yield loss of about 612%. Over the years, platforms for screening of ShB resistance has been established, but to date no rice accession has been found to be immune against the disease. It appears that ShB resistance is quantitatively governed by many minor genes and is affected by two factors: physiological resistance and disease escape. In our study, a set of 130 rice accessions with different backgrounds were phenotyped using detached tiller assay (DTA) method under controlled condition. Each rice accession was screened for lesion height, number of lesions and maximum lesion length. In addition, we explore a genome wide association approach based on 3798 single nucleotide polymorphism (SNP) variants, to speed up the identification of quantitative trait loci controlling resistance to ShB using Trait Analysis by aSSociation, Evolution and Linkage (TASSEL) program. SNPs with significant associations on the selected traits of this rice germplasm and the contribution of this approach to rice breeding strategies will be discussed.

I. CANICOSA , G. Dimayuga , J. 1 1 1 Chatterjee , M. Dionora , W.P. Quick

CaPTUring VariaTiOn in riCe PHOTOSynTHeSiS 1

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C4 Rice Center, International Rice Research Institute, Los Banos, Philippines Rice is currently the most important crop species and the primary food crop for more than half of the worldÕs population. Presently, there is a tremendous need to increase rice yield to cope with the increasing food requirement. To do this, rice needs to have a more efficient photosynthetic system in a less water and less fertilizer condition. Hence, a thorough study of the photosynthetic nature of Oryza family for both the domesticated and wild rice species is necessary to understand how to achieve this goal. In this study, photosynthesis and other related traits were measured using the LI-6400 Infra-Red Gas Analyzer. Results revealed a huge variation in the photosynthesis and transpiration of different species in Oryza. This variation is partly due to the different growing habit and partly due to the domestication effect. Overall, there are approximately two-fold changes in the light saturated maximum photosynthesis (Pmax) among the species that have the highest or lowest photosynthetic rates. Related traits also vary accordingly. Highest Pmax of IR64 suggests that the cultivated rice has reached its full photosynthetic potential. A reconstruction of structure and

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biochemistry is needed to further boost rice yield capacity.

plant type varieties as a donor parent. QTLs for yield-related traits were successfully detected. improving root system development Improving root system development which is less attention to that of plant type is expected to be effective trait for stable rice production. The strategy, i.e., finding novel QTL/genes and developing materials for breeding, will be described. nutrient efficiency and problem soil tolerance Genome-wide association studies are used to identify novel genetic sources for enhanced nutrient efficiency. Breeding efforts for improved phosphorus uptake using recently identified P uptake gene OsPSTOL1 will be focused on. Multi-location trial with ir64 introgression lines A multi-location trial is ongoing to evaluate yield performance in Asia and Africa using IR64 introgression lines under the collaboration of JIRCAS-IRRIAfricaRice. Mitigation of heat-induced sterility at anthesis Early-morning flowering trait is effective approach to mitigate heat-induced spikelet sterility in the era of global warming. The breeding activity and research network will be described.

P0077 JirCaS reSearCH aCTiViTieS reLaTeD TO riCe geneTiCS in griSP 1

2

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T. Ishimaru , T. Ishimaru , Y. Fukuta , N. 2 3 2 Kobayashi , N. Kobayashi , M. Obara , 2 2 M. Wissuwa , S. Yanagihara 1

Plant Breeding Genetics and Biotechnology Division, International Rice Research Institute, Los Baños, Philippines 2 Biological Resources Division, Japan International Research Centre for Agricultural Sciences (JIRCAS), Ibaraki, Japan 3 Institute of Crop Science, National Agriculture and Food Research Organization, Ibaraki, Japan Japan International Research Center for Agricultural Sciences (JIRCAS), as a strategic partner of the Global Rice Science Partnership (GRiSP), plays a role as focal point in Japan and encourages rice genetic research collaboration of GRiSP program. The following JIRCAS research activities will be presented. rice blast research network "Blast research network for stable rice production" has been conducted for targeting development of differential system for pathogenicity of blast isolates and resistance of rice variety in Asia. enhancement of yield potential To improve yield potential, introgression lines of IR64 were developed using new

P0078 Dna anaLySiS reVeaLS HigH aMOUnT OF MOLeCULar VariaTiOn anD POPULaTiOn DiFFerenTiaTiOn in arOMaTiC riCe LanDraCeS (Oryza SaTiVa L. SSP inDiCa) FrOM UTTar PraDeSH, inDia

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landraces, four Basmati and two genotypes of O. nivara and O. rufipogon. The eight base-pair deletions (8-bp del) in the exon-7 of BADH2 gene along with three SNPs were found to occur both in long grained Basmati and medium grained non-Basmati aromatic rices. The findings of present study are more relevant to rice improvement specially the aromatic types as there is large amount of genetic variation is present in the accessions analyzed.

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M. Yadav , M. Mittal , S. Tiwari , V. 2 3 Yadav , K. Srinivasan 1

Division of Genomic Resources, National Bureau of Plant Genetic Resources, Delhi, India 2 Department of Genetics and Plant Breeding, CS Azad University of Agriculture and Technology, Kanpur, India 3 Division of Germplasm Conservation, National Bureau of Plant Genetic Resources, Delhi, India

P0079

Rice (Oryza sativa L.) is one of the most important staple food crops feeding more than half of the worldÕs population. There has been a wide range of interest in identifying the genetic variation between Basmati and non-Basmati aromatic types. A panel of 44 landraces of aromatic rice (O. sativaL. ssp. indica) currently being grown in the states of Uttar Pradesh and Manipur were molecularly analyzed using 38 microsatellite loci. The 38 primer-pairs amplified a total of 285 alleles with an average of 7.5 alleles per microsatellite locus. The average frequency of unique or private alleles was 0.151, whereas the frequency of rare alleles was 0.204. A large number of genotypes (287) exhibited two alleles per genotype, while 14 genotypes showed amplification of three alleles per genotype. The presence of two or more alleles per SSR locus within an accession reveals the heterogeneous nature of landrace, which was due to its genetic composition. The partial region of exon-7 of aroma gene BADH2 was amplified from 50 accessions of aromatic rices that included 44 non-Basmati aromatic

TaLUSi: an HDF5 TOOL FOr eFFiCienT STOring anD QUerying OF Large SnP DaTaSeTS 1

1

analysis involves complicated steps using various software that has its own input and output formats. We implented Minokawa to simplify the steps for effecient comparative genome analyis. Methodology: Minokawa is a pipeline for automated, high-throughput comparative analyses of whole genome sequences. It is designed to support marker development for breeding and genetic diversity studies. Minokawa includes standard open source software applications for read filtering and quality trimming (fastx toolkit), read mapping (bowtie, bwa), de novo genome assembly (SOAPdenovo, velvet) and visualization of genome data (gbrowse, gbrowse_syn). After setting the parameters for each software through a configuration file, the pipeline can be ran unattended and output an annotated draft genome. Minokawa can be installed locally and run via the command line interface or can be integrated into galaxy as a workflow. results: Input to Minokawa are short sequence reads in fastq or fasta formats while the output is an annotated draft genome ready for visualization. On the average the pipeline can produce an annotated draft genome in eigth to sixteen hours depending on the sequencing depth and the hardware used. Discussions and Conclusions: Minokawa provides an easy to use interface for comparative analyses of draft genomes. The pipeline saves time and increases the efficiency for analyses.

1

R.R. Fuentes , K. McNally , R. Mauleon , 1 1 V.M. Juanillas , N. Alexandrov

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vcftools. Using HDF5 data model, variant calls with rich annotations can be managed with well-structured and compressed storage and fast I/O. By integrating FastQuery, data can be indexed for conditional queries on large sets of samples. results: Talusi supports comparisons, subset queries, merging, and conditional searches with significant performance gain and size reduction. Discussion and Conclusions: With Talusi, we also demonstrate how HDF5 platform can efficiently handle large SNP genotyping data sets with hundreds of thousands of markers even with a common desktop machine. Migrating data to HDF5 gives better and flexible platform for future tool development. P0080

1

TTC- Genetic resource center, International Rice Research Institute, Los Baños, Philippines

MinOKaWa: a PiPeLine FOr COMParaTiVe anaLySeS OF DraFT genOMeS

introduction: Data formats like VCF and Hapmap are developed, together with tools for specific analysis and queries, to store DNA polymorphism data such as SNPs. As genotyping datasets grow in size and complexity, new approaches that provide faster access and more efficient storage are needed. Hierarchical Data Format (HDF5), a technology suite used by the scientific community to manage extremely large and complex data collections, can serve as alternative method to store genotype data. Methodology: Talusi is a suite of tools that loads VCF or Hapmap to HDF5 and enables queries like that provided by

V.J. Ulat , F.N. Borja , J. Detras , V. 1 1 Juanillas , R.R. Fuentes , R.J.R. 1 1 2 Santos , R. Mauleon , P. Tanger

1

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TTC- Genetic resource center, International Rice Research Institute, Los Baños, Philippines 2 Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, USA introduction (Background/Purpose): Comparative genome anlysis enables scientists and researchers to identify regions of similarity and difference between different rice varieties. However the

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P0081

documented as well. From the population that we studied, a correlation between some of the anatomical and morphological alterations was observed. Back-crossing of mutants with wild type and polymorphic parent are being done to clean up the gene pool before genome sequencing to identify candidate genes.

FinDing C4 TO C3 reVerTanTS in a MUTageniSeD SeTaria ViriDiS POPULaTiOn By STUDying anaTOMiCaL LeaF aBerraTiOnS 1

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N. Larazo , G. Rizal , K. Acebron , R. 1 1 Garcia , W.P. Quick 1

C4 Rice Center, International Rice Research Institute, Los Baños, Philippines

P0082 LOSS-OF-FUnCTiOn OF an UBiQUiTin-reLaTeD MODiFier rUrM1 PrOMOTeS THe MOBiLizaTiOn OF THe aCTiVe MiTe MPing

It is common knowledge that C4 plants are more efficient than C3 plants in terms of photosynthetic activities and water-use under high light intensity and high temperature conditions. These efficiencies are being of more importance nowadays when threats to worldÕs food security such as global warming and climate change are palpable. One factor for C4 plantÕs photosynthetic efficiency is its unusual leaf structure known as Kranz anatomy. It has been proven many times that Kranz anatomy plays a key role in the photosynthetic process of C4 plants in which it provides a site where CO2 can be concentrated around RuBisCO, thus avoiding photorespiration. While our focus is to find C4 to C3 revertants for C4 gene identification that are responsible for Kranz anatomy, we came across a number of phenotypes expressed by Setaria viridis mutants. Using Gamma ray and N-Nitroso-Nmethylurea as mutagens, we started studying about 4,400 mutated lines of S. viridis. Among these, we found at least 59 lines that deviate from the normal C4 leaf structure. Morphological phenotypes from these 59 lines were observed and

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T. Tsukiyama , S. Teramoto , K. Yasuda , 2 1 1 A. Horibata , N. Mori , Y. Okumoto , M. 1 1 1 Teraishi , H. Saito , A. Onishi , K. 1 1 Tamura , T. Tanisaka 1

Graduate School of Agriculture, Kyoto University, Kyoto, Japan 2 Faculty of Biology-Oriented Science and Technology, Kinki University, Wakayama, Japan Transposable elements (TEs) are able to promote evolution by causing genome rearrangements and by altering the structure and regulation of individual genes. Among various kinds of TEs, miniature inverted-repeat transposable elements (MITEs) are widespread in both prokaryotic and eukaryotic genomes, where their copy numbers can attain several thousands. Little is known, however, about the genetic factor(s) affecting their transpositions. Here we show that disruption of a gene encoding ubiquitin-like protein markedly enhances

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the transposition activity of a MITE mPing in intact rice plants without any exogenous stresses. We found that the transposition activity of mPing is far higher in the lines harboring a nonfunctional allele at the Rurm1 (Rice ubiquitin-related modifier-1) locus than in the wild-type line. Although the alteration of cytosine methylation pattern triggers the activation of transposable elements under exogenous stress conditions, the methylation degrees in the whole genome, the mPing-body region, and the mPing-flanking regions of the nonfunctional Rurm1 line were unchanged. Our results clarify one of the transposition strategies of MITEs in nature, namely, MITEs can actively mobilize when the host cells suffer not only from exogenous stress but also from endogenous stress. As such, this study provides experimental evidence for one of the models of genome shock theory that genetic accidents within cells enhance the transposition activities of transposable elements.

Introduction: Background/Purpose Rice (Oryza sativa L.) suffers both drought stress and sheath blight infection caused by Rhizoctoniasolani. Genetic overlaps between the drought tolerance (DT) and sheath blight resistance (SBR)were found in breeding materials, however, thepossible genetic mechanism remains unclear. Methodology A pyramiding line, PT94 with DT and SBR and its recurrent parents, IR64, as well as the relatedpedigrees materials were used for phenotyping and genotyping following the standard methods.Overlapping dissection for DT and SBR were carried out by both QTL mapping and transcriptional profiling. Results Based on the QTL mapping results, of the 15 SBRrelated bins, the percentage of overlap between SBR and DT would be 40%. In transcriptional profiling, of the significantly transcribed genes under SB inoculation, much lower overlaps were found in IR64 (4.2%) and a pyramiding lines, PT94 (1.6%). Of the 40 genes overlapped between DT and SB in IR64, of them 7 (17.5%) were located within the overlap regions of DT and SBR QTL identified. Discussions and Conclusions Overlaps were found at both the QTL and the transcriptional profiling levels; the correspondences between evidences from these two levels would help elucidating the functional genes underlying the genetic overlaps between DT and SBR. However, itÕs notable that fewer differential expressed genes were found in PT94 than IR64. This could

P0083 geneTiC OVerLaPS BeTWeen DrOUgHT TOLeranCe anD SHeaTH BLigHT reSiSTanCe in riCe (Oryza SaTiVa L.) 1

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T. Zheng , W. Wang , L. Hoang , F. Li , L. 1 1 1 1 1 Huang , L. Zhu , Y. Sun , J. Xu , Z. Li 1

Molecular Biology, Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences, Beijing, China

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be another possible mechanism underlying the overlap of DT and SBR in PT94.

Association analysis Based on the mixed linear model considering population structure and family relatedness [i.e. the (Q + K) model], was done. The False Discovery Rate (FDR) method was used for correction multiple testing.The present study reports nineteen significant marker-trait associations, involving 11markers for eleven morphologic traits.5 markers were associated with flag leaf traits (flag leaf length, flag leaf width and flag leaf area) whereas three marker was responsible for all three grain traits (grain length, grain width and Grain length/ width ratio) variation. Moreover we found markers which associated with days to heading, Plant Height, 1000grain weight and number of total and filled grain per panicle. These markers can be informative for rice breeding programs through marker-assisted selection.

P0084 aSSOCiaTiOn MaPPing OF yieLD anD DiFFerenT agrOnOMiC TraiTS in STrUCTUreD POPULaTiOn OF riCe genOTyPeS 1


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M. Jahani , G. Nematzadeh , G. 2 Mohammadi-Nejad 1

Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, Iran 2 Plant Breeding, Shahid Bahonar University of Kerman, Kerman, Iran Association mapping is a different approach to identify quantitative trait loci (QTLs) by examining the marker-trait associations in populations based on linkage disequilibrium. In this study. Association analysis was performed in a global panel of rice accessions, consisting of 100 rice; lines, cultivars and genotypes. The population was phenotyped for 15 morphological traits and genotyped using 81 molecular markers including: sixty-seven SSRs markers located on the 12 chromosomes and mainly around qFL1,qFLW4 and GS3 regions. Seven sequence tagged site (STS) markers adjacent to qpn1, two Indel, two CAPS (cleaved amplified polymorphic sequence), two SSR and a PCRbased SNP marker related to GS3 were used. A model-based population structure analysis divided the rice materials into three subpopulations.

P0085 reSearCH FOr BreeDing OF eXPOrT riCe VarieTieS FOr VieTnaM 1

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L. NGUYEN THI , L.U.Y. Trinh thi Luy , 2 2 X.A. Tran thi Thanh , P. Tam bui , C.H.I. 2 Buu bui 1

plant breeding and genetics, Institute of Agricultural Sciences for Southern Vietnam, Ho Chi Minh, Vietnam 2 plant breeding and genetics, Cuulong delta rice research Insistue, Ho Chi Minh, Vietnam A total of 332 rice varieties (300 traditional rice varieties and 32 short

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P0086

duration and high-yielding rice varieties) including introduced rice varieties involving to the quality of rice plant were analyzed. The result of analysis indicated that amylose content was recorded at low (16-22%) and average (25%) level whereas gel consistency revealed low variability from 48-72mm. It is also interesting to mention that alkali spreading value was variable at 3-7 level. Based on above mentioned results about gel consistency, such as a suggestion, gel consistency improvement in short duration and highyielding rice varieties need to be made. Selection of elite rice varieties was on single-cross and backcross method as well as on the basis of quantitative genetics and interaction of rice varieties evaluated through genetic index in breeding. The analysis of hybrid generations of each BCF2, BCF2:3 populations has gained new knowledge and horizons in the number of genes controlling agronomically important traits such as yield and chalkiness. The valuable contributions in genetic basis of genes rice quality and other important ones: (1) increasing high quality rice varieties, (2) improving rice yield considerably from 7-8 tons, (3) inducing genetic bases to be difficult to control. Two hundreds crossing and 12.600 lines from various generations were selected. Mostly, rice varieties produced in this project had long grains, high filled-grain ratio as OM6377, OM4900, and OM5930. Rice varieties with low amylase content and having aroma were also identified as OM4900, OM6900, OM6161, OMCS2009; and OM4900, OM 6600, OM 6161 respectively.

aSSOCiaTiOn BeTWeen aLLeLeS OF THe FragranCe gene anD arOMaTiC COMPOUnDS in aSian riCe (Oryza SaTiVa L.) 1

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J.C.T. Concepcion , C.C. Llorente , V.D. 2 1 Daygon , N. Sreenivasulu , M.A. 2 Fitzgerald 1

Grain Quality and Nutrition Center, International Rice Research Institute, Los Baños, Philippines 2 School of Agriculture and Food Sciences, University of Queensland, Brisbane, Australia Rice aroma is highly-valued by many even in an evolving global rice market and consumer preferences. Due to the appealing sensory quality, aromatic rices are recognized at large and are continuously given priority despite of the higher cost as compared to nonaromatic rices. The demand for quality rices has driven various stakeholders to breed for aromatic rices in high-yielding backgrounds. This method is actively pursued with the accurate identification of the genetic basis of rice fragrance and development of allele-specific molecular markers that can detect the introgression of the fragrance gene. Molecular markers has aided in detecting a novel mutation in Asian rice (Oryza sativa L.) and validated the th biggest alteration on the 7 coding region of rice chromosome 8. This 806base pair deletion across exons 4 and 5 was found to be the cause of the higher levels of the volatile compound 2-acety1-pyrroline (2AP) as detected by coupled Gas Chromatography and Mass

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Spectrometry (GC-MS) in the varieties containing the mutation as compared to those carrying the common mutation. Volatile metabolic compounds responsible for the fragrance of F7 recombinant-inbred lines of two Lao PDR traditional varieties were screened by GC-GC-MS. This study will elucidate on the gene discovery, marker validation and untargeted discriminative metabolomic analysis of the basis of rice fragrance. With a single gene, different alterations provide unique blends of metabolites, thus, scent. It is an opportunity to dissect the fragrance trait at wider perspective to filter into IRRI's rice improvement strategies.

of parboiled rice specially kernel whiteness, hardness, broken grain percentage and head rice yield. The quality of parboiled rice varies from place to place, in spite of paddy variety, parboiling treatments such as soaking procedures and steaming procedures are similar. Soaking water quality is varying in place to place and country to country due to its dissolved chemical such as CaCO3, Cl, NO3 and Fluoride irons. However, CaCO3 is the most commonly dissolved chemical in ground water. It can be varied 250mg/L to 3800mg/L according to the place and country. Hence, research forced mainly on investigating, how dissolved CaCO3 in soaking water affects the quality of parboiled rice. The results in revealed, CaCO3 concentration soaking water significantly changes the whiteness of rice kernel of parboiled rice. CaCO3 concentration in the soaking water did not significantly affect other quality parameters such as kernel hardness, broken grain percentage and head rice yield percentage. However, increase of CaCO3 concentration in soaking water significantly improved kernel whiteness up to some extent, but the relationship was not linear.

P0087 eFFeCT OF DiSSOLVeD CaLCiUM CarBOnaTe in SOaKing WaTer FOr ParBOiLeD riCe QUaLiTy C. Gunathilake

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Research and Development Center, Institute of Post Harvest Technology, Anuradhapura, Sri Lanka

Generally, parboiling process of rough rice/paddy consists of three stages: soaking paddy in water to saturate the gain with water, gelatinization of rice starch by adding heat to the moist kernels through steaming, and drying up to moisture content suitable for milling or storage. The demand of the parboiled rice depends on quality characteristics such as kernel whiteness and hardness (texture). Soaking water quality, soaking procedures and steaming procedures are main factors for altering the qualities

P0087 eFFeCT OF DiSSOLVeD CaLCiUM CarBOnaTe in SOaKing WaTer FOr ParBOiLeD riCe QUaLiTy C. Gunathilake 1

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Research and Development Center, Institute of Post Harvest Technology, Anuradhapura, Sri Lanka

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kernel whiteness up to some extent, but the relationship was not linear.

Generally, parboiling process of rough rice/paddy consists of three stages: soaking paddy in water to saturate the gain with water, gelatinization of rice starch by adding heat to the moist kernels through steaming, and drying up to moisture content suitable for milling or storage. The demand of the parboiled rice depends on quality characteristics such as kernel whiteness and hardness (texture). Soaking water quality, soaking procedures and steaming procedures are main factors for altering the qualities of parboiled rice specially kernel whiteness, hardness, broken grain percentage and head rice yield. The quality of parboiled rice varies from place to place, in spite of paddy variety, parboiling treatments such as soaking procedures and steaming procedures are similar. Soaking water quality is varying in place to place and country to country due to its dissolved chemical such as CaCO3, Cl, NO3 and Fluoride irons. However, CaCO3 is the most commonly dissolved chemical in ground water. It can be varied 250mg/L to 3800mg/L according to the place and country. Hence, research forced mainly on investigating, how dissolved CaCO3 in soaking water affects the quality of parboiled rice. The results in revealed, CaCO3 concentration soaking water significantly changes the whiteness of rice kernel of parboiled rice. CaCO3 concentration in the soaking water did not significantly affect other quality parameters such as kernel hardness, broken grain percentage and head rice yield percentage. However, increase of CaCO3 concentration in soaking water significantly improved

P0088 anTiOXiDanT anD anTiMiCrOBiaL PrOPerTieS OF CHaK-HaO - a DarK PUrPLe gLUTinOUS arOMaTiC riCe OF ManiPUr, inD ia. M. Kangabam

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Medicinal plant and Horticultural Division, Institute of Bioresources and Sustainable Development, IMPHAL, India introduction: Dark purple rice is considered to be a healthy food in Asia. 'Chak-hao', a dark purple glutinous aromatic rice of Manipur is famous for its aesthetic and neutraceutical properties. The anthocyanin content of purple rice has got a strong biological activity. Methodology: Different extracts of the local purple rice were evaluated for their antimicrobial activity against the human pathogenic Gram positive and Gram negative bacterial and fungal pathogens using the disc*diffusion method. Antioxidant activity was determined by DPPH method (Shimada et.al. 1992). results: The MIC of the methanol and petroleum extract against the tested bacterial and fungal pathogen was found to be 5 µg and 12 µg respectively. The methanolic extract of Chak-hao gives promising antioxidant activity with IC50 8.5 µg/ml as

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Institute, Science City of Munoz, Philippines

compared with the standard sample Lascorbic acid with IC50 4.5 µg/ml. Discussion and Conclusion: The petroleum and methanol extract showed strong antibacterial activity against Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus with the inhibition zone diameter ranging from 11 to 22 mm whereas water extract showed less activity against the test pathogens. No activity was found against P. aeruginosa and S. aureus. Methanol extracts of purple rice exhibited strong activity against Candida albicans and Aspergillus flavus. The results obtained in this study confirm that purple rice has got a promising source of bioactive molecules having antimicrobial activity.

Introgression lines of betacaroteneenriched rice 'Golden Rice' event R (GR2R) in the background of IR64 and PSB Rc82 were obtained from IRRI and subjected to confined field testing at PhilRice, Science City of Mu–oz with the approval and under the regulatory supervision of the Department of Agriculture-Bureau of Plant Industry. The materials were composed of 38 introgression lines of IR64 at BC3F3, 32 lines of PSB Rc82 at BC3F1, and 10 lines of PSB Rc82 at BC2F3. Evaluation focused on the following parameters: morpho-agronomic characteristics, reaction to insect pests and diseases, genetic similarity to the recurrent parent based on 373 genome-wide SNP markers, and total carotenoid content after two months of storage at ambient temperature. Among the IR64-GR2R lines, 32 were selected as closely resembling the wild type IR64 based on phenotypic acceptability and morphoagronomic characteristics. The selected IR64-GR2R lines had 75 to 126 cm plant height, 80 to 86 heading days, 101 to 106 maturity days, and 5.17 to 11.20 ug/g total carotenoid content as opposed to 7.65 ug/g in the donor and negligible amount (0.42ug/g) in the recurrent parent. Among the PSB Rc82-GR2R lines, 13 were identified as similar to wild type PSB Rc82, having 92 to 132 cm plant height, 81 to 86 heading days, 104 to 111 maturity days, and 80.5 to 90.5% recurrent parent genome recovery. Based on these results, selected lines were subjected to generation advance, phenotypic selection of plants within lines, and multi-location field evaluation

P0089 COnFineD FieLD TeST OF PrOViTaMin a enriCHeD 'gOLDen riCe' eVenT gr2-r inTrOgreSSeD LineS OF ir64 anD PSB rC82 1

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A.A. Alfonso , J.J. Somera , E.O. 1 1 1 Espejo , R.T. Miranda , E.S. Avellanoza , 1 1 D.A. Tabanao , E.S. Nazareno , N.C. 3 3 Ramos , M.V. Romero 1

Plant Breeding and Biotechnology Division, Philippine Rice Research Institute, Science City of Munoz, Philippines 2 Research and Development Department, Monsanto Philippines, General Santos City, Philippines 3 Rice Chemistry and Food Science Division, Philippine Rice Research

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to evaluate agronomic performance and to obtain regulatory data.

sticky cooked rice. To resolve these issues, an attempt to introgress beta carotene gene/locus from japonica transgenic Golden rice into BRRI dhan29, the most popular irrigated rice variety of Bangladesh was undertaken and successfully completed. The selected introgression lines were found identical to BRRI dhan29 by 97% to 99% for SSR and 91.4% to 96.2% for SNP marker loci. Some of these lines were imported from IRRI and evaluated in a contained screenhouse in Bangladesh. Carotenoids contents in polished grains of these lines were up to 12 µg/g at 2 months of storage at ambient temperature, which is sufficiently enough to meet at least 50% of the average requirement of vitamin A for VAD affected people in Bangladesh. If Golden Rice is found to be safe as food and feed, and efficacious, this can be used as a sustainable approach to reduce VAD among vulnerable simultaneously with existing other approaches

P0090 MarKer aSSiSTeD inTrOgreSSiOn OF gOLDen riCe TraiT inTO a Mega riCe VarieTy OF BangLaDeSH 1

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P. Biswas , M.A.H. M. A. Hossain , M.I. 2 2 M. Inabangan , J.M. J. Macabenta , V.A. 2 2 V. Aldemita , V.L. V. Lacorte , L.T. L. 2 2 3 Torrizo , M.S. M. Samia , J.T. J. Tan , 2 4 R.B. R. Boncodin , T.S.B. T. S. Bharaj , 2 4 G.B. G. Barry , P.V. P. Virk 1

Plant Breeding Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh 2 PBGB, International Rice Research Institute, Los Banos, Philippines 3 CAS Lib, CAS, Hunan, China 4 Harvest Plus, ICRISAT, Patancheru Andhra Pradesh, India

P0091

Rice is the staple food grain in Bangladesh. A major portion of the population takes more than 400g rice daily. But rice does not contain beta carotene, the precursor of vitamin A, which results severe vitamin A deficiency (VAD) among poor people, especially children and pregnant women, who have limited access to other non-rice vitamin A-enriched foods. Golden rice (GR), a new type of rice developed by Prof. Ingo Potrykus and Peter Bayer contains beta carotene. But these Japonica-type GR varieties are not acceptable to farmers and consumers due to poor agronomic performances in tropical conditions of Bangladesh and

riCe BiOTeCHnOLOgy: aPPLiCaTiOn OF MiCrOSaTeLLiTe MarKerS FOr SCreening anD iDenTiFiCaTiOn OF irOn riCH riCe genOTyPeS 1

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L. HASSAN , G.S. Jahan , S.N. Islam , 1 1 M.S. Alam , R. Gain 1

Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh, Bangladesh Rice (Oryza sativa L.) is the most important food crop of th

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e developing world and the staple food of more than half the global population. Widespread production and consumption of such crop could reduce micronutrient defici encies, improve health outcomes, and provide economic benefits. Unfortunately, mineral contents are low in many of the staple food crops.The deficiency of micronutrients such as iron (Fe) in staple food crops is the most common nutritional disorder specially for pre-school children and pregnant woman in Bangladesh. Therefore, screening of iron rich rice genotypes is an essential study on the context of Bangladesh.In this study 52 rice genotypes were analyzed for iron (Fe) concentration by using Atomic Absorbance Spectrophotometer (AAS) at 248.33 nm. Digestion of rice grain samples were carried out by diacid mixture of HNO3 and HCIO4 (2:1) Iron concentration ranged from 1.32 ppm to 100.45 ppm. The highest iron concentration was found in Lal Gotal and the lowest was in Jota Balam. The local landraces had the highest iron (Fe) concentration. Molecular screening of iron rich rice genotypes was carried out by using ten SSR markers. Among the ten markers, RM17, RM21 and RM400 had showed the polymorphism with 52 rice germplasm. Similar to chemical analysis, local landraces were also performed better in iron content than the cultivated varieties. Thus, local landraces can be the good source for biofortification of popular rice cultivars using crop improvement programs.


P0092

P0093

Fine MaPPing OF THe LOWaMyLOSe CHaraCTeriSTiCS OF riCe CULTiVarS BaegJinJU

TarOM geneTiCaLLy MODiFieD riCe iS nOn aLLergeniC

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M.H. Nam , J. Lee , Y.J. Hur , S.Y. Kim , 1 1 1 D.S. Park , J.H. Cho , J.Y. Lee , Y.B. 1 1 Sohn , Y.C. Song 1

Department of Functional Crop, Natioal Institute of Crop Science, Mirynag, Korea

Control of the amylose content is a major strategy for breeding rice with improved eating quality. To identify the low-amylose gene of the low-amylose rice cultivar Baejinju derived from MNU treatment, Genetic analysis was conducted on 200 F2 indiviual plants derived from a cross between the japonica cultivars Milyang244 and Baegjinju 1(a donor parent of Baegjinju). of Individual F2 plants Milyang244/Baegjinju were classified as wild type or mutant type(opaque grain) based on the grain appearance of brown rice. A total of 200 F2 plants from Milyang244/Baegjinju segregated into 155 wild type , and 45 mutant type, 2 which fit to a 3:1 ratio and (χ = 0.667, df = 1, p = 0.414), suggesting the inheritance of a recessive gene for amylase content. The low amylose gene locus du1(t) was finely mapped within an approximately 800kb region between 17.5 to 18.8Mb on the short arm of chromosome 10, and simple sequence repeat(SSR) markers RM25648 was cosegregating with the locus. This DNA marker developed in the candidate region of the du1(t) locus are useful tools for low amylose gene.

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A. Najaf

neither significant alignment nor similarity of Cry1Ab protein at full sequence, domain, and epitope level with any of the known allergens. Our results indicate Cry1Ab protein has no allergenic property based on bioinformatics analysis.

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Bioinformatics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

P0094 TOCOPHerOL anD TOCOTrienOL COnTenT in KOrean JaPOniCa anD TOngiL TyPe riCe

Rice (Oryza sativa) is the staple food in most countries. So safety evaluation of genetically modified (GM) rice is very important for consumers. One of the most important issues in the consumption of Genetically Modified Plants (GMPs) is to ensure safety, health and non-allergenicity of them. Allergy is an important health issue, which is caused by certain food proteins, including proteins derived from GMPs. Tarom GM rice has been created using Cry1Abgene transfer from Bacillus thuringiensis. Cry1Ab gene expresses a delta-endotoxin protein which is toxic to rice stem borer larvas. The use of bioinformatics screening for allergenicity assessment of novel proteins is recommended by the world health organization, the European Food Safety Authority (EFSA), and the US Environmental Protection Agency. Therefore, Bioinformatics analysis was implemented in order to study allergenicity of Cry1Ab protein. Cry1Ab protein sequence was aligned to seven allergen databases including FARRP, SDAP, ADFS, Allergome, Algpred, PSD, and Allermath. Sequence alignment was performed with the allergen proteins by three approaches including the full sequence, the 80 amino acids, and the 6-8 amino acids. The results showed

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J.S. Lee , M.R. Yoon , J. Kwak , J.H. 1 2 1 3 Lee , S.W. Yoon , A. Chun , J. Lee , I.H. 2 Kim 1

Rice Research Division, National Institute of Crop Science Rural Development Administration, Suwon, Korea 2 Korea University, Department of Food and Nutrition, Seoul, Korea 3 Chungbuk National University, Department of Food Science and Technology, Cheongju, Korea Functional rice oils are known as tocopherols and tocotrienols. Tocotrienols have received greater attention than tocopherols because of the biological activities such as cholesterol reduction, skin protection and antioxidant activity. By the previous research, tocotrienols is 4-40 times more effective as antioxidant than vitamin E, α-tocopherol. The content of tocols in the 23 Japonica type and 4 Tongil type of rice cultivars harvested between 2010 and 2011 were investigated. The rice oil was extracted from 1 kg of brown rice flour using 2.5 L of n-hexane for 3 h at

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ambient temperature. n-Hexane was evaporated by a rotary evaporator o (Eyela, Japan) at 30 C. HPLC chromatographic separation was performed by a normal phase Lichrospher Si-60 column (250 mm × 4.6 mm i.d Merck Co.) and each peak was detected at an excitation wavelength of 298 nm and an emission wavelength of 325 nm by a JASCO FP1520 fluorescent detector (Jasco Co., Japan). Four tocopherol homologues, i.e., α-, β-, γ-, and δtocopherol, and three tocotrienol homologues, i.e., α-, γ- and δ-tocotrienol were identified. The total content of tocols in the 27 cultivars were detected in a range 14.86~38.19mg/kg brown rice. The tocopherols content of Japonica type cultivars were higher than those of Tongil type cultivars. Meanwhile, tocotrienols content of Tongil type cultivars were higher than those of Japonica type cultivars. The major tocols homologues in the 23 cultivars of Japonica type were αtocopherol, while that in Tongil type cultivars namely Dasan 1, Hanareum, Segyuejinmi, and Cheonseog was γ-tocotrienol.

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RIce Research Division, National Institute of Crop Science(NICS) Rural Development Administration(RDA), Suwon, Korea

Rice (Oryza sativa L.), as a staple food in Korea, is usually blended with white rice and brown rice for healthy eating habits and due to its nutritional value. Ten (10) Korean rice varieties of Japonica type suitable for brown rice blending with low-energy input at cooking were eavaluated. In 2011 cropping season, all cultivars were grown and harvested under the same environmental conditions at the National Institute of Crop Science, Rural Development Administration, Korea. Brown rice was obtained by dehulling rough rice with a dehuller (SY88-TH, SSANG YOUNG, Korea). The physicochemical compounds, water absorption rate, hardness of brown rice kernels and the texture properties of cooked brown rice were also measured using Tensipresser (My Boy Systems model, Japan) by cultivar. Amylose, protein, and dietary fiber contents of brown rice cultivars ranged from 5~20%, 6.6-8.7%, 6.3~12.4%, respectively. The optimum soaking time of brown rice before cooking was minimum six hours, with 90% moisture content and lower kernel hardness. As regards the textural properties of cooked brown rice, the hardness of Boseokchal, Baekjinju and Seolgaeng exhibited the higher value among the rice cultivars. Based on the results, relatively low hardness of brown rice with good eating quality can contribute to reduce the cooking time. Therefore, Boseokchal, Baekjinju and Seolgaeng are suited for

P0095 inVeSTigaTiOn OF WaTer aBSOrPTiOn, COOKing anD TeXTUre PrOPerTieS OF BrOWn riCe FOr LOW-energy inPUT USing Ten KOrean CULTiVarS OF JaPOniCa TyPe 1

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A. Chun , M.R. Yoon , J.H. Lee , S.K. 1 1 1 1 Oh , J. Kwak , J.S. Lee , B.K. Kim

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results: A major QTL controlling GT, named qGT-6, explaining ~ 40% of phenotypic variance has been discovered on Chr. 6 in the marker interval, RM217-RM276, spanning a physical distance of ~ 2 Mb. Later, the QTL has been fine mapped to a smaller genomic interval of ~ 1 Mb in between the markers, RM217-RM19562. The QTL was detected in another population segregating for GT and was observed to be highly stable, when tested in multiple environments. We also discovered QTLs controlling GC, AC and L/B ratio and these are being validated in alternate populations. Discussions and Conclusion: A novel and stable QTL, qGT-6 has been reported for the first time in this study with a high PV. The QTLs discovered in our study can be deployed in markerassisted breeding programs aimed at grain quality improvement.

brown rice blending because of their low-energy input at cooking, rapid water absorption capacity, short cooking time, lower hardness of brown rice, and lower gelatinization temperature. P0096 iDenTiFiCaTiOn anD MOLeCULar MaPPing OF MaJOr QTLS COnTrOLLing PHySiCO-CHeMiCaL QUaLiTy TraiTS in riCe 1

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S. MAGANTI , S. Ramadevi , B. 1 1 1 Venkaiah , S.K. Hajira , K. Pranathi , S. 1 1 Srikanth , V. Ravindra Babu , R.M. 1 1 Sundaram , N. Shobha Rani , B.C. 1 Viraktamath 1

BIOTECHNOLOGY, Directorate of Rice Research-ICAR, HYDERABAD, India introduction: Improvement of rice grain quality is considered as an important breeding objective, next to yield. Rice quality is defined by various physicochemical parameters and their genetic control is polygenic. In the present study, we have attempted to map major QTLs controlling the grain quality traitsgelatinization temperature (GT), gel consistency (GC), amylose content (AC), kernel length, length/breadth (L/B) ratio and kernel elongation after cooking (KLAC). Methodology: RIL mapping populations developed from crosses between indica parents differing significantly for above mentioned traits were used. Molecular mapping was carried out using ~ 500 SSR markers covering the whole genome using the software Mapmaker/QTL.

P0097 iMPrOVing OrganiC PHOSPHaTe aCQUiSiTiOn in TranSgeniC riCe 1

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M. Nam , S. Park , D. Shin , D. Park , D. 2 1 Kim , W. Hwang 1

Department of Functional Crop, Natioal Institute of Crop Science, Mirynag, Korea 2 Department of Genetic Engineering, Dong-A University, Busan, Korea Phosphorus is essential element for plant growth and development. The orthophosphate (Pi) content in soil for plant growth is usually very low, organic phosphate (Po) which plant cannot use

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account for 30~80% of total P in agricultural soils. Here we developed two kinds of transgenic rice which can hydrolyze Po by over-expressing an acid phosphatase 1 (ACP1-OX) and puple acid phosphatase (PAP-OX). We evaluated the activity of phosphatase, growth characteristics and phosphate acquisition of ACP1-OX and PAP-OX under Po condition. As result of staining of acid phosphatase activity in root, both transgenic rices showed much darker and more intense purple color than wildtype plant under Po treated nutrient solution. By analyzing remained total phosphate content of nutrient solution after three days cultivation, both transgenic rices uptake more phosphate than wild-type plant under Po treated nutrient solution. As the result of pot treatment, the growth characters of both transgenic rice and wild-type plant were similar in control and no Pi treated condition. The tiller number and plant height of wild-type plant in Po treated condition were similar as those in no Pi treated condition. However, tiller number and plant height of transgenic rice were similar as those in control condition. These results suggest that overexpression of acid phosphatase 1 and puple acid phosphatase gene is an effective approach to improve organic phosphate acquisition in rice.

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Department of Agriculture, Rice Research and Development Institute, Ibbagamuwa, Sri Lanka

Basmathi rice constitutes a small but special group of rice which is considered best in quality. This rice has long been popular in the orient, and now becoming more popular in the Middle East, Europe and the United States. One of the major constraints on the development of Basmathi rice is the low yield potential due to the traditional plant type. Incorporation of semi dwarf plant type to existing plant type is difficult due to high sterility because of the incompatibility between semi dwarf indica and the traditional basmathi varieties. To overcome this problem, mutation breeding and biotechnological approaches have been utilized by the breeders and a number of basmathi lines with semi dwarf background have been developed. These newly improved varieties are more compatible than semi dwarf indica types with the traditional basmathi cultivars. We evaluate newly developed semi dwarf basmathi rice germplasm from international rice research institute, Philippines for aroma, intermediate amylose content, acceptable kernel length and breath, standard kernel elongation ratio and gelatinization temperature etc. Several lines such as IR 75482-41-1-2, IR 75482-235-2, IR 75484-101-1, IR 75484-189-2, IR 75490-79-1, IR 7286024-2, IR 71731-20-4, IR 71146-407-2, IR 74717-207-2, IR 75478-34-1 and IR 67406-6-3 were selected as superior basmathi lines which can be utilized as parental material in basmathi rice

P0098 evaluation of semi dwarf Basmathi rice (Oryza sativa L.) germplasm for quality rice improvement G. Abeysundara Hewawasamge

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improvement breeding programs in future. Key words: semi dwarf, basmathi rice, incompatibility, grain quality

and carbon dioxide content rose to 2.4% and 2.7 % in the hermetic IRRI-super bag after 4½ and 9 months of storage respectively. The results showed that MC of the poly-sack stored paddy increased (p 80%), stigma length (> 2.0 mm), stigma breadth (>0.35 mm) and anther length (>2.0 mm). Other promising TGMS lines identified were TNAU 19S, TNAU 50S, F4-17S, F4-32S, CBTS 0282-5, MS 1,

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U. Verma , O.P. Verma , M. Kumar , G.P. 1 1 1 1 Verma , P.K. Singh , A. Devi , Y. Kumar , 1 1 1 P.K. Yadav , S. Dwivedi , R.R. Tiwari , 1 D.K. Dwivedi 1

Genetics and Plant Breeding, N.D. University of Agriculture and Technology, Faizabad, India

The efficacy of few selected combinations for stimulatory heterotic effects on yield and its contributory components in hybrid rice has been tested following line X tester analysis. The analysis of variance revealed that mean sum of squares due to treatments, parents and crosses had significant differences, whereas, significance of parents vs. crosses indicated the presence of heterotic effects in crosses. Results revealed that based on relative pollen sterility and filled grains percentage all the three CMS lines (IR58025A, IR68897A and IR79156A) having ÒWAÓ cytoplasm were found stable. Wide range of heterotic effects were observed for various quantitative

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traits indicating involvement of diverse genotypes in cross combinations. The top five restores identified were IR55179-3B-11-3, NDRK 50035, NDRK 5059, CSR 28 and NarendraUsarDhan 2008. Hybrids viz., IR68897A x AT401, IR58025A xCSR28, IR68897AxIR72049B-R-22-3-1-1, IR79156Ax IR55179-3B11-3 and IR79156Ax IR72049-B-R-22-31-1 exhibited significant heterobeltiosis for yield and its components. Hybrids viz., IR68897A xAT 401, IR58025AxAT 401, IR68897Ax IR72049-B-R-22-3-1-1, IR79156AxIR55179-3B-11-3 and IR58025 AXIR55179-3B-11-3 exhibited standard heterosis over NarendraUsar 3 for yield component traits. IR68897AxAT 401 and IR58025AxAT 401 exhibited standard heterosis over KRH 2 for the traits viz.,flag leaf area, plant height, panicle length and grain yield per plant. In the present study , the identified above top five restorers certainly had potentiality for generating high heterotic cross combinations for most of the yield contributing traits and be utilized to enhance yield potential in sodic soil. The significant values of heterosis or hybrid vigour obtained in these crosses have been attributed to non additive types of genetic interaction.

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Genetics and Plant Breeding, N.D. University of Agriculture and Technology, Faizabad, India

Sodicity is a major environmental stress impediment for growth and production of rice. Three cytoplasmic-genetic male sterile (CMS) lines of rice having wild abortive (WA) cytoplasmic male sterility source were crossed with twelve diverse genotypes to identify their fertility restorer and / or maintainer nature. The experiment was laid out in randomized complete block design with three H replications at p =9.6 and EC= 2.21 -1 dSm . The data was recorded on thirteen quantitative traits. Results revealed that majority of the genotypes expressed differential fertility restoration estimates when crossed with these CMS lines. Among the genotypes tested twenty-five were found to be common as effective restorers for all the CMS lines. The promising top ten in order of merit were IR 58025A × AGAMI MI, IR 58025A × NDRK 50035, IR 79156A × IR 551793B-11-3, IR 68897A × Narendra Usar Dhan 2008, IR68897A × AT 401, IR 58025A × IR 55179-3B-11-3, IR 68897A × IR555179-3B-11-3, IR 68897A × CSR 28, IR68897A × IR 55179-3B-11-3 and IR 79156A × Narendra Usar Dhan 2008. These promising genotypes may be tested for hybridity for development of rice hybrids under sodicity. Remaining eleven genotypes produced partial restorer hybrids with all the three CMS lines which may be utilized for developing novel sources moving towards male sterile lines under the salt affected soil.

P0209 iDenTiFying SUPeriOr FerTiLiTy reSTOrerS anD MainTainerS FOr CMS LineS OF riCe (Oryza SaTiVa L.) UnDer SODiCiTy 1

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U. Verma , O.P. Vrema , G.P. Verma , M. 1 1 1 Kumar , P.K. Singh , D. Dwivedi

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SV1; crosses IR 68897A × Narendra usar 3, IR 58025A × Narendra usar 2, IR 80555A × Narendra usar 2, IR 58025A × NDRK 5018 and IR 80555A × Susk Samrat over SV2 and crosses IR 58025A × Narendra usar 2, IR 68897A × Narendra usar 3, IR 80555A × Narendra usar 2, IR 58025A × NDRK 5018 and IR 80555A over SV3 for grain yield and some other contributing traits. These crosses are suggested for cultivation in target environments after testing and release.

STUDieS On HeTerOTiC POTenTiaL in riCe (Oryza SaTiVa L.) FOr yieLD anD iTS COMPOnenTS USing CMS SySTeM 1

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S. Dwivedi , P.K. Singh , O.P. Verma , A. 1 1 2 Devi , R. Dwivedi , D. Dwivedi 1

Genetics and Plant Breeding, N.D. University of Agriculture and Technology, Faizabad, India 2 Biotechnology, N.D. University of Agriculture and Technology, Faizabad, India

P0211 aSSOCiaTiOn OF QUanTiTaTiVe TraiTS in riCe (Oryza SaTiVa L.) UnDer SODiC COnDiTiOn

The investigation was carried out to study the heterosis in rice in a set of 54 hybrids (F1s) and their 21 parents along with three checks for seventeen characters under sodic condition (pH 9.8 -1 and EC 2.34 dSm ) in randomized block design with three replications during Kharif, 2011. Heterobeltiosis ranged from -100.00 to 239.99% and standard heterosis varied from -100.00 to 274.40% over Pusa RH 10 (SV1), from -100.00 to 206.14% over Narendra 118 (SV2) and -100.00 to 157.78% over Sarjoo 52 (SV3) for grain yield per plant. The best five cross combinations were IR 68897A × Narendra Usar 3, IR 68897A × Pusa Sugandha 3, IR 58025A × Narendra Usar 2, IR 68897A × NDRK 5018 and IR 80555A × Narendra Usar 2 which showed significant positive heterobeltiosis; crosses IR 68897A × Narendra usar 3, IR 58025A × Narendra usar 2, IR 80555A × Narendra usar 2, IR 58025A × NDRK 5018 and IR 80555A × Susk Samrat showed significant and positive standard heterosis over

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O.P. Verma , A.H. Khan , D. Dwivedi

line x tester mating design to develop the experimental material. Results revealed that overall epistasis and its Ò j Ó and Ò l Ó types components were significant for majority of the traits. The fixable additive x additive (i) type epistasis was significant for panicle bearing tillers per plant, L/B ratio, biological yield and harvest index. The additive as well as the dominance components of genetic variation was significant for spikelets per panicle, filled spikelets per panicle, biological yield, days to 50% flowering, plant height, 1000- grain weight , spikelets fertility and panicle length. Additive component was significant for all the traits except harvest index, indicating thereby considerable scope of improvement in these traits by following conventional breeding procedure leading to development of pure line varieties. Dominance component was significant for majority of the traits suggested that improvement in these traits may be greater through heterosis breeding. All the traits showed partial dominance possessing more than one value of degree of dominance.The directional element of dominance was non significant for majority of the traits, suggested ambidirectional nature of dominance except positive and significant value for panicle length, days to 50% flowering and 1000- grain weight, indicating directional dominance with alleles having increasing effect with mostly recessive in nature.

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1

Genetics and Plant Breeding, N.D. University of Agriculture and Technology, Faizabad, India 2 Crop Physiology, N.D. University of Agriculture and Technology, Faizabad, India 3 Biotechnology, N.D. University of Agriculture and Technology, Faizabad, India Correlation and path coefficients for 12 quantitative traits involving fifty diverse rice genotypes with different genetic background and maturity groups were H evaluated in three replications at p =9.7 -1 and EC= 2.67 dSm at MES, NDUAT, Faizabad during wet season 2012. The -1 grain yield plant was found to be associated positively and significantly at both genotypic and phenotypic levels -1 with biological yield plant and flag leaf

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area. Further, the perusal of data revealed that certain traits were intercorrelated with each other. Moderate to high estimates of broad sense heritability and genetic advance -1 was observed for biological yield plant , -1 number of spikelets panicle and 1000 grain weight which indicates involvement of additive gene action. Path coefficient analyses revealed that biological yield serves as the most important grain yield contributing traits substantiated by indirect effect through flag leaf area, spikelet fertility, effective tillers plant 1 and panicle length. Hence, planed selection for the above quantitative traits would be the positive motivations to increase biological yield vis-a-vis enhanced grain yield productivity of sodic soil. P0212 gene aCTiOn FOr grain yieLD anD iTS COMPOnenTS in riCe (Oryza SaTiVa L.) UnDer SODiC SOiL 1

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G. Dharwal , O.P. Verma , G.P. Verma , 1 1 1 P.K. Singh , D.K. Dwivedi , Y. Kumar , 1 1 P.K. Yadav , R.R. Tiwari 1

Genetics and Plant Breeding, N.D. University of Agriculture and Technology, Faizabad, India The modified triple test cross analysis was applied to estimate additive, dominance and epistatic components of genetic variance for twelve traits in rice. Three testers CSR10, Jaya and their hybrid CSR10x Jaya, were crossed with seven diverse strains of rice following

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P0213

NDRK 50027, IR 68897A× NDRK 50026, IR 58025A × NDRK 50035 and IR 58025A× NDRK 50033, indicated the involvement of non-additive gene action. These cross combinations possess high × high and high × low indicating the preponderance ofadditive as well as non-additive gene actions. Higher magnitude of sca variance than gca variance, greater degree of dominance and lower predictability ratio further confirmed the significant role of nonadditive gene action for majority of the quantitative traits. The above cross combinations possessing high heterotic effects needs to be tested on large scale for commercial exploitation of heterotic potential to improve yield potential under sodic soil.

COMBining aBiLiTy FOr yieLD anD iTS COnTriBUTing COMPOnenTS in riCe (Oryza SaTiVa L.) UnDer SODiC SOiL 1

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P.K. Yadav , O.P. Verma , G.P. Verma , 1 2 1 P.K. Singh , D.K. Dwivedi , K.K. Mishra , 1 1 V.K. Mishra , A. Devi 1

Genetics and Plant Breeding, N.D. University of Agriculture and Technology, Faizabad, India 2 Biotechnology, N.D. University of Agriculture and Technology, Faizabad, India Combining ability analysis for yield and its components was carried out following line × tester (2 ×12) analysis to sort out desirable parents and/ or crosses for commercial exploitation under sodic soil. The analysis of variance revealed that both general as well as specific combining ability variances were significant for all the t quantitative characters, indicating wide genetic variability among the parents along with cross combinations. Results further revealed that estimates of gca was higher than the estimates ofsca indicating preponderance of additive gene action for majority of the characters. Among the female lines IR 58025A was found to be good general combiner for yield and its attributes. Among the male lines NDRK 50026, NDRK 50038, NDRK 50033, NDRK 50032 were judged as good general combiners for grain yield and its components. The promising good specific combiners, identified were IR 68897A× NDRK 50019, IR 98897A×

P0214 geneTiC VariaBiLiTy anD COrreLaTiOn STUDieS in inDigenOUS anD eXOTiC geneTiC STOCKS OF riCe (Oryza SaTiVa L.) 1

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R. Yadav , O.P. Verma , G.P. Verma , 2 1 1 D.K. Dwivedi , P.K. Singh , K. Singh , 1 1 1 R.K. Chaudhary , P.K. Yadav , A. Devi , 1 R.R. Tiwari 1

Genetics and Plant Breeding, N.D. University of Agriculture and Technology, Faizabad, India 2 Biotechnology, N.D. University of Agriculture and Technology, Faizabad, India A field experiment with 35 diverse genotypes of rice including Sarjoo 52 and Narendra Usar Dhan 3 as check was evaluated in randomized

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P0215

block design with 3 replications. Analysis of variance revealed existence of wide genetic variability amongst the genetic stock. High magnitude of genotypic and phenotypic coefficient of variation was observed for biological yield per plant followed by plant height and grain yield per plant. Results revealed that least variations were observed between the genotypic as well as phenotypic coefficient of variability in majority of the quantitative traits, indicating the least influence of the environmental factors. High magnitude of heritability coupled with high genetic advance was observed for biological yield per plant followed by plant height, grain yield per plant and flag leaf area indicating involvement of additive gene action; suggesting direct selection of these traits for rice improvement in sodic soil. Grain yield per plant exhibited a very strong significant positive association with biological yieldper plant followed by flag leaf area and spikelet fertility percentage at phenotypic as well as genotypic levels.Further, path coefficient analysis reflected that biological yield per plant, harvest index, days to 50% flowering and spikelets per panicle were the major direct contributors while flag leaf area, effective tillers per plant, spikelets per panicle, spikelet fertility percentage, days to maturity, plant height, panicle length and 1000-grain weight showed main indirect contributors for grain yield. Hence, emphasis should be given to select these traits to enhance the production and productivity of rice in salt affected soil.

COMBining aBiLiTy FOr yieLD anD iTS COMPOnenTS in riCe (Oryza SaTiVa L.) USing CMS SySTeM 1

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S. Dwivedi , P.K. Singh , O.P. Verma , A. 1 1 2 Devi , R. Dwivedi , D. Dwivedi 1

Genetics and Plant Breeding, N.D. University of Agriculture and Technology, Faizabad, India 2 Biotechnology, N.D. University of Agriculture and Technology, Faizabad, India A set of 54 F1s and their 21 parents along with three checks were evaluated for seventeen characters under sodic -1 condition (pH 9.8 and EC 2.34 dSm ) in randomized block design with three replications during Kharif, 2011. On the basis of general combining ability effects, the testers Narendra Usar 2, Narendra Usar 3, Susk Samrat, NDRK 5018, Pusa Sugandha 3, T 9 and NDR 6262 and line IR 68897A were identified as a good general combiners for yield and several other important traits to emerge as valuable parents for hybridization program for obtaining high yielding hybrid varieties. The top five cross combinations viz., IR 58025A × NDR-1119, IR 58025A × Narendra usar 2, IR 58025A × NDRK 5018, IR 58025A × IR 64 and IR 58025A × IR 29 showed significant and positive sca effects for grain yield as well as for some other yield components. The crosses exhibiting high order of significant and desirable sca effects for different characters involved parents having all types of combinations of gca effects such as high × high, high ×

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average, high × low, average × average, average × low and low × low general combiner parents, indicating the involvement of all types of gene actions . The foregoing observations clearly indicated that there was no particular relationship between positive and significant sca effects of crosses with gca effects of their parents for the characters under study.

resources culminating in development and release of modern-day high yielding varieties. In the present study 2,962 rice germplasm accessions were characterized for 18 qualitative and 13 quantitative traits as per the standard evaluation system of IRRI. The data was subjected to simple correlation analysis and the correlation coefficients for various morphological traits were determined. The results revealed that seedling height was positively correlated with leaf length, days to 50 percent flowering, plant height, grain length, grain width and test weight and negatively correlated with leaf width, number of productive tillers and grain yield. Similarly, plant height recorded significant positive correlation with leaf length (0.486), leaf width (0.250), days to 50 % flowering (0.326), panicle length (0.349) and grain length (0.168) and negatively correlated with number of productive tillers and grain yield. Summarising the results, the criteria of preferring/selecting shorter seedlings to longer seedlings which often lodge at maturity saves valuable land resources and increases the selection efficiency.

P0216 COrreLaTiOn anaLySiS OF riCe gerMPLaSM anD iTS BreeDing aPPLiCaTiOnS 1

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C. Uddaraju , L.V. Subba Rao , G. Shiva 1 1 1 Prasad , K. Suneetha , N. Shobha Rani , 2 2 N.K. Gautam , S. Kalyani 1

Crop improvement, Directorate of rice research, HYDERABAD, India 2 Evaluation division, NBPGR, New Delhi, India Rice is the basic dietary requirement for majority of the world population particularly in Asia and also the most diverse crop cultivated in the world with more than 1,08,000 germplasm collections in the Genebank of International Rice Research Institute and an equal number i.e 95,000 accessions in the national genebank of NBPGR, New Delhi. The objective of the experiment is to characterise the available germplasm and utilise the data to establish meaningful correlations among the agro morphological traits which can be further utilised in establishing a selection mechanism thus conserving time and

P0217 yieLD reSPOnSeS OF riCe genOTyPeS FOr DrOUgHT TOLeranCe in rainFeD LOWLanD SiTUaTiOn


P0218

Drought at reproductive stage regularly affects the productivity of rice in Bihar where more than seventy percent rice area comes under rain-fed situation. An experiment comprised of fifteen rice genotypes including local checks of medium maturing groups were evaluated in kharif, 2012 at the university farm under normal and reproductive stage drought situations in Alpha-lattice design with three replications in 20x20 centimetre spacing. Significant differences among the genotypes were found for yield and other characters under study. Maximum tensiometer reading was found 37 and water table depth 90.5 centimetres during the stress condition. During the flowering month (October) the total rain was 41mm. The highest yield was found for the genotype IR 87753-11-2-12 followed by IR 84895-B-127-CRA-5-11, IR 87751-20-3-2-1, IR 83387-B-B-274 and IR 83373-B-B-24-3. These all five genotypes exhibited significantly higher yielder in comparison to best local check Rajendra Sweta (2113 kilogram/hectare) under stress situation. None of the genotypes exhibited significantly high yield than the best check. Minimum yield reduction of 15.06% was observed for IR 83387-B-B-27-4 under stress in comparison to normal situation; however it was highest in check Shambha Mahsuri (71.17%). These genotypes may fulfil the gap of availability of drought tolerant varieties for the farmers of Bihar.

eVaLUaTiOn OF THe eFFeCT OF SPr3 LOCUS CaUSing SPreaDing On yieLD-reLaTeD TraiTS in riCe 1

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Department of Agronomy College of Agriculture and Life Sciences, Chungnam National University, Daejeon, Korea

introduction: Introduction of agronomically important genes from wild relatives and traditional rice varieties to modern rice varieties are often faced with linkage drag problem. Here, we report the linkage of the durable blast resistance gene Pi45(t) with SPR3 causing spreading panicle and the effect of SPR3 locus to yield-related traits in rice. Methodology: Pi45(t) was previously mapped using a NIL of Ilpumbyeo containing a segment of Moroberekan at chromosome 4. To further delimit the gene region, the NIL was backcrossed with Ilpumbyeo. Using CNRB3 and RM17579 markers linked to Pi45(t) and SPR3, respectively, we genotyped F-2 population and selected recombinant plants. Progenies of selected F2 plants were planted at two different locations, having two replicates per location. Traits such as panicle length, spikelet per panicle, number of primary branch per panicle, number of secondary branch per panicle and 1,000-grain weight will be evaluated this season.

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S. Singh 1

Plant Breeding and Genetics, Bihar Agricultural University, Bhagalpur, India

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M.E. Fabreag , D.M. Kim , S.M. Yeo , 1 S.N. Ahn

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results: Based on the genotypes of 304 F2 plants, the genetic distance between Pi45(t) and SPR3 loci is ~6.4cM, which agrees with the genetic distance inferred from the physical distance between the two loci, which is 1.87Mb. Agronomic traits will be evaluated this season. Discussions and Conclusions: These results suggest the linkage, but not complete, of Pi45(t) and SPR3. This provides the opportunity to utilize Pi45(t) in breeding programs.

were showed that panicle lenght, width and lenght of leaf blade, plant height and yied have a high of heritability. Mostly of promising lines have average higher than CD value of between check and variety. It is indicate that they were significantly superior than check. The top three of best lines were BP10622FBB4-15-BB4, Weed Tol Rice 1 and Zhongzu14. They were produced yield around 4.67, 4.48 and 4.37 t/ha, respectivelly. The best line were well adapted in the rainfed area.

the yield of 6.0 to 9.0 tons/ha from hybrid giving distinct advantage of 15 to 20%. Still hybrid area coverage is less than 5%, only 2.0 mha out of 44.0 mha area under rice since 1994. BAU has started developing new generation of hybrids with higher yield, better quality and higher seed yield. Some of the promising three-line hybrid rice varieties preferred by the farmers across the agro-climatic zones in the State are Arize Tej, PAC 801, 27P31, Arize 6444, PHB-71 and KRH-2.

P0219

P0220

P0221

geneTiC ParaMeTer STUDy aMOng riCe PrOMiSing LineS

CUrrenT STaTUS anD FUTUre PrOSPeCTS OF HyBriD riCe in JHarKHanD, inDia

HyBriD riCe reSearCH anD DeVeLOPMenT in egyPT

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I. Rumanti , I. Mulsanti

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M. Pandey , D.N. Singh

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Plant Breeding, Indonesian Center for Rice Research, Subang, Indonesia Hundred promising inbred lines were developed through conventional breeding to anticipate the climate change and fulfill feed for people in Indonesia. The lines were breed using some of donor such as ultra early maturity, good quality, resistant to brown plant hopper and bacterial leaf blight, tolerant to drought and acid sulphate toxicity. The research was conducted during 2012 in Sukamandi, West Java. Augmented design with 9 check varieties were used in this research. The major purpose of the research was to study the genotypic and phenotypic coefficient variation, and heritability value of broad sense. The analysis was conducted followed Sharma et al (2006) method. The result

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cultivar (Giza 178) regarding tolerance to biotic and abiotic stresses, and grain quality expressed as grain appearance, milling recovery, nutrient value and panel taste test. Year 2005 is the start year for hybrid rice cultivation in Egypt on a commercial basis. In addition to these hybrids, second and third stage hybrid combinations are in the development pipeline. In Egypt, the three-line breeding method for hybrid rice is used; however, the towline methos Thermo-Sensitive Genic Male Sterility (TGMS) and Photoperiod Sensitive Genic Male Stirility (PGMS) is used too. Hybrid rice seed production is a tedious work and requires high level of accuracy and to obtain cost effective seed production. The environment in Egypt appears to favor high out-crossing, seed setting and seed quality. The average seed yield under experiment plots is now 2.8 t/ha.

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H. El-Mowafi , A.M.R. Reda , R. 1 1 1 Abdallah , E. Arafat , O. El-Badawi

1

Department of Plant Breeding and Genetics, Birsa Agricultural University, RANCHI, India

1

Rice Research and Training Center, Field Crops Research Institute, Kafr ElSheikh, Egypt

Since start of hybrid rice research in December 1989, India has earned the distinction of being the second largest hybrid rice growing country. Jharkhand state has more than 1.8 million ha are under rice with productivity of 2.0 tons/ha. A total of 29 and 31 hybrids are developed respectively, by public and private institutions in India and more than 95% hybrid seeds are produced by private sector. The current area under hybrid rice in the state is more than 3.5 lakh ha. BAU has tested about 200 hybrid varieties under three diverse agro-climatic zones during past three years. A large number of demonstrations in farmersÕ field explicitly demonstrate

The hybrid rice research program in Egypt was started in 1995 supported by collaborative projects involving USA, FAO and IRRI. By 2004, almost all components of the hybrid rice technology program were completed by producing the certified hybrid rice seed, which was made available for commercial cultivation of hybrid rice in farmerÕs fields during 2005. The program resulted in the development of tow promising hybrid rice combinations; SK 2034H (EHR 1) and SK2046 (EHR 2), which out yielded the current commercial cultivars by 1530 %, meanwhile possessed a similar performance to that of the most popular

P0222 iDenTiFiCaTiOn OF arOMaTiC HyBriD riCe USing MiCrOSaTeLLiTe (SSr) MarKerS in egyPT. 1

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H. El-Mowafi , A.M.R. Reda , R. 1 Abdallah 1

Rice Research and Training Center, Field Crops Research Institute, Kafr ElSheikh, Egypt Aromatic rices constitute a small special group of rices which are considered best in quality. If aromatic hybrid rice is

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preferred, it is suggested that both parents of hybrids should be aromatic. Four microsatellite (SSR) markers (RG28, RM8264, RM7049 and RM23120) linked to fragrance (fgr) gene were used in this study to identify 12 aromatic and non aromatic parents (five CMS and seven restorers) and some F1 aromatic hybrids comparing with Basmati 370 as aromatic check variety. The genotypes PR78R, PR2R, IR68888A and IR70368A (the first two genotypes are considered as restorer and the last two genotypes are considers as CMS) gave aromatic banding pattern with tow SSR markers ( RG28 and RM23120) similar to Basmati 370 indicating that these nonpredicted aromatic genotypes are have the same allele related to the fragrance gene. On the other hand, the nonpredicted aromatic genotypes are having superior agronomic performance particularly earliness and grain yield. The combinations PR78R/ IR68888A and PR2R/ IR68888A gave aromatic banding pattern with the same tow markers similar to Basmati 370. Results showed that SSR markers were found to be highly polymorphic between parents and F1 hybrids.

1

Plant Breeding Genetics & Biotechnology, International Rice Research Institute, Los Baños, Philippines

Existing varieties currently available for direct seeding are mostly prone to poor seedling establishment when there is flooding immediately after sowing or when there is poor, uneven land preparation before seeding. Hence, developing rice varieties having tolerance for anaerobic germination stress under zero or minimal oxygen during flooding will be beneficial to farmers practicing direct seeding as these varieties can germinate, elongate, and thrive even under flooded conditions. Breeding for anaerobic germination stress has been intensified in the past few years. Intercrosses were made among traditional lines, varieties or near isogenic lines with some popular varieties and improved IRRI lines. These were generally semi-dwarf to intermediate in stature, have erect plant type, and have good grain quality, tolerance to some other abiotic stresses such as submergence, salinity or drought, and tolerance to some biotic stresses including bacterial leaf blight and tungro. In our trait-based breeding program, F2 and F3 populations have been screened for anaerobic germination stress under field conditions. Only the selected survivals are advanced to the next generations for further selection in the pedigree nurseries. At present over 600 F6 lines are ready for further screening for anaerobic germination tolerance under field conditions. Yield testing will also be

P0223 DeVeLOPMenT OF iMPrOVeD LineS WiTH TOLeranCe TO FLOODing aT gerMinaTiOn STage FOr DireCT SeeDeD riCe eCOSySTeMS 1

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C. Casal , J.A. Tarun , J.C. Ignacio , A. 1 1 1 Pamplona , D.J. Mackill , A.M. Ismail , 1 E.M. Septiningsih

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conducted in the coming plantings. Hopefully, our products can be utilized by IRRI core breeders or directly evaluated and further advanced by our national partners giving rise to new varieties that can thrive in flood-prone direct seeded areas.

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SPONSORS & EXHIBITORS

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SPONSORS’ PROFILES DuPont Pioneer Company Profile DuPont Pioneer is the world’s leading developer and supplier of advanced plant genetics, providing high-quality seeds to farmers in more than 90 countries. Pioneer provides agronomic support and services to help increase farmer productivity and profitability and strives to develop sustainable agricultural systems for people everywhere. In Asia, Pioneer began its hybrid rice breeding program in India in 1987 following the successful adoption of hybrid rice technology and increased rice yields in China. Today, Pioneer offers commercial hybrids of differing maturities, areas of adaptation, and grain quality preferences across India, Indonesia, Philippines, Pakistan and Vietnam.

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EXHIBITION OPENING HOURS

EXHIBITORS’ PROFILES

The commercial exhibition is open on the following dates and time:

DuPont Pioneer

Date

1 Harbour Front Place #11-01 Harbour Front Tower One, Singapore Tel: +65 6586 3015 Fax: +65 6272 7494 Contact: Prabdeep Bajwa E-mail: [email protected]

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Tuesday, 5 November

09:00 – 18:30


08:00 – 18:30


08:00 – 18:30

Friday, 8 November

Stand No. #04

CLOSED

Company Profile

EXHIBITORS LIST Company

Stand No.

DuPont Pioneer

#04

Guill-Bern Corporation

#A3

International Rice Research Institute (IRRI)

#03

Japan International Research Center for Agricultural Sciences

#05

KETT Electric Laboratory

#A1

DuPont Pioneer is the world’s leading developer and supplier of advanced plant genetics, providing high-quality seeds to farmers in more than 90 countries. Pioneer provides agronomic support and services to help increase farmer productivity and profitability and strives to develop sustainable agricultural systems for people everywhere. In Asia, Pioneer began its hybrid rice breeding program in India in 1987 following the successful adoption of hybrid rice technology and increased rice yields in China. Today, Pioneer offers commercial hybrids of differing maturities, areas of adaptation, and grain quality preferences across India, Indonesia, Philippines, Pakistan and Vietnam. Guill-Bern Corporation

Stand No. #A3

14 San Francisco cor. San Rafael Sts., Bo. Kapitolyo, Pasig City Philippines 1603 Tel: +632 746 1470 Fax: +632 746 0886 Contact: Bernadette D.Sia E-mail: [email protected] Company Profile Guill-Bern Corporation, proudly a wholly Filipino-owned company, is a distributor of scientific and analytical equipment for microbiology, biotechnology, life science, quality assurance and environmental laboratories since 1991. With partner suppliers from Europe, US, Japan, Korea and China, we aim to provide the most advanced, high quality products and systems. We aim to work in excellence and strive to exceed the expectations of our customers by providing the highest levels of service and customer care.

492

493


International Rice Research Institute (IRRI)

Stand No. #03

Los Baños, Philippines Tel: +63 2 580 5600 (ext 2204 or 2587) Email: prstaff _AT_ irri.org


KETT Electric Laboratory

Stand No. #A1

1403, 1-Chome, 4-8, Nishi-gotanda, Sinagawa-ku, Tokyo 141-0031, Japan Contact: Norihiro Yoshida E-mail: [email protected]

Company Profile The International Rice Research Institute (IRRI) is a non-profit independent research and training organization. IRRI is a member of the CGIAR Consortium. IRRI develops new rice varieties and rice crop management techniques that help rice farmers improve the yield and quality of their rice in an environmentally sustainable way. We work with our public and private sector partners in national agricultural research and extension systems in major rice-growing countries to do research, training, and knowledge transfer. Our social and economic research also informs governments to help them formulate policy to improve the equitable supply of rice. Japan International Research Center for Agricultural Sciences

Company Profile Kett measuring devices are indispensable pieces of equipment that reliably perform important jobs in all fields in which accuracy and precision are important. Many Kett products have been adopted as standard measuring devices in Japan and are being used in a wide range of fields. We like to think of our measuring devices as “extensions of the 5 human senses” and continue to strive in our efforts to provide reliable products for all measuring requirements.

Stand No. #05

1-1 Ohwashi, Tsukuba, Ibaraki Ken, Tsukuba Tel: 63-2-580-5600 Fax: 63-2-580-5699 Contact: Tsutomu Ishimaru E-mail: [email protected] Company Profile As a strategic partner of the Global Rice Science Partnership (GRiSP), JIRCAS serves as a focal point in Japan and encourages rice genetic research collaboration as part of its GRiSP program. Its research activities focuses on rice blast research network, enhancement of yield potential, improving root system development, nutrient efficiency and problem soil tolerance, multi-location trial with IR64 introgression lines and mitigation of heat-induced sterility at anthesis.

494

495


AUTHORS INDEX

497

1


A. Henry 57, 59, 63, 66, 271, 279, 300, 312 A. Higashitani 99, 447 A. Horibata 85, 386 A. Hussaini 60, 284 A. Ismail 14, 15, 46, 53, 69, 70, 226, 253, 323, 324, 325 A. Jha 12, 133 A. Joomwong 93, 421 A. Kerhornou 13, 135 A. Khanna 78, 79, 361, 362 A. Kohli 12, 13, 41, 59, 210, 279 A. Konaté 14, 140 A. Kotasthane 57, 271 A. KOTASTHANE 75, 345, 346 A. Kumar 7, 25, 45, 55, 57, 58, 59, 66, 67, 117, 157, 223, 264, 271, 275, 279, 312, 313 A. KUMAR 105, 473 A. La Hoang 69, 321 A. Lahiri Majumder 14, 139 A. Lazaro 39, 199 A. Mabilangan 31, 32, 82, 101, 172, 176, 375, 455 A. Macovei 45, 223 A. Maji 14, 140 A. Marshall 35, 186 A. Masuduzzaman 57, 273 A. McClung 96, 433 A. Min 41, 210 A. Miyao 42, 211 A. Mokhtar 33, 178 A. Mori 22, 51, 148, 248 A. MS 63, 300

A A. Abe 78, 361 A. Amparado 45, 92, 224, 418 A. Amresh Kumar Nayak 58, 276 A. Anandan 7, 117 A. Anupam 57, 271 A. AUDEBERT 54, 257 A. Aumeeruddy 24, 156 A. Aye Min 59, 279 A. Azhar 48, 235 A. Banerjee 30, 171 A. Barretto 50, 241 A. Beena 51, 247 A. Birah 97, 438 A. BISWAS 72, 333 A. Bordeos 74, 339 A. Chun 87, 89, 395, 396, 404, 405 A. DARDOU 54, 257 A. De Luna 36, 37, 42, 188, 194, 213 A. DeBures 3, 114 A. Dela Cruz 78, 358, 359 A. Devi 106, 107, 476, 478, 480, 481 A. DIEVART 44, 54, 220, 257 A. Dobermann 1 A. Elmido-Mabilangan 39, 200 A. Erban 25, 158 A. Estiati 80, 367 A. Francisco 39, 44, 199, 220 A. Goodyear 25, 157 A. Grover 15, 142 A. Hairmansis 52, 250 A. Han 43, 218 A. Hartana 22, 149

497 499


A.A. Efisue 104, 467 A.A. Thant 59, 280 A.A.T. Johnson 24, 153 A.A.Y. Amarasinghe 62, 291 A.B. Shikari 78, 79, 361, 362 A.C. De Luna 39, 44, 199, 220 A.D. Barretto 51, 61, 245, 290 A.G. Sajise 50, 241 A.G.C. Sajise 51, 61, 62, 64, 245, 290, 295, 302 A.H. Khan 49, 106, 239, 478 A.J. Bogdanove 73, 338 A.J. Greenberg 8, 26, 124, 161 A.J. JOEL 72, 332, 333 A.K. Biswals 59, 279 A.K. Dohling 65, 308 A.K. Misra 30, 171 A.K. Singh 7, 55, 65, 68, 77, 78, 79, 105, 117, 262, 265, 304, 317, 355, 361, 362, 473 A.K. Tripathi 69, 322 A.K. Tyagi 39, 201 A.K.P. Sivaranjani 24, 154 A.L. Baniqued 43, 216 A.M. Bossa-Castro 76, 353 A.M. Corales 94, 423 A.M. Delos Reyes 31, 172 A.M. Ismail 55, 61, 64, 65, 66, 108, 262, 263, 288, 301, 307, 308, 486 A.M. McClung 26, 161 A.M.R. Gatehouse 41, 210 A.M.R. Reda 108, 485 A.N. Monroy 32, 176 A.N. Vispo 50, 241 A.P. Bentota 97, 100, 436, 451 A.Q. Feng 77, 354

A. Nabipour 36, 188 A. Najaf 87, 395 A. Nakada 40, 204 A. Olson 13, 135 A. Onishi 85, 386 A. Palanog 55, 264 A. Pamplona 108, 486 A. Pandey 7, 117 A. Parco 10, 129 A. Pareek 14, 69, 92, 139, 322, 323, 418 A. Pattanayak 30, 171 A. Pereira 19, 120 A. Pérez-Quintero 20, 145 A. Prasad 50, 244, 294 A. Prasad Babu 60, 62, 286 A. Ramanantsoanirina 9, 127 A. Ramli 33, 94, 178, 426 A. Sajise 33, 179 A. Sharan 14, 139 A. Shikari 60, 284 A. Singh 10, 55, 58, 62, 79, 128, 265, 275, 292, 363 A. Siti 88, 399 A. Srividhya 56, 266 A. Tagle 38, 198 A. Tanaka-Kawasaki 71, 95, 327, 431 A. Tomita 53, 257 A. Treumann 41, 210 A. Tsuyu 44, 221 A. Velmurugan 55, 262 A. YAMAUCHI 48, 234 A. Yoshimura 21, 29, 37, 78, 147, 165, 166, 193, 360 A. Yugander 21, 73, 146, 337 A. Zannati 70, 325 A.A. Alfonso 27, 86, 163, 392


A.S. Hari Prasad A.S. Hariprasad A.S. Vidyarthi

B. Matthews 44, 219 B. Niranjan 68, 317 B. Panda 33, 177 B. Phuoc Tam 70, 325 B. RADHA 59, 279 B. Ravikumar 77, 357 B. Sah 72, 334 B. Sena Munuswamy 39, 50, 201, 242 B. Shaw 33, 177 B. Siddiqua 48, 235 B. Sreevani 75, 344 B. Srikanth 33, 180 B. Szurek 20, 145 B. Venkaiah 87, 397 B. Wanjala 48, 234 B. Yuan 20, 145 B.A. VEERESHA 37, 104, 192, 469 B.C. Marandi 49, 239 B.C. VIRACKTMATH 37, 192 B.C. Viraktamath 10, 11, 24, 33, 38, 53, 73, 79, 87, 88, 154, 180, 196, 254, 335, 337, 364, 397, 401 B.C. VIRAKTMATH 91, 414 B.C. Viratktamath 97, 437 B.C.V. Viraktamath 21, 146 B.D.R. Prasantha 88, 399 B.E. Huang 103, 463 B.G.D.S. Weerasinghe 62, 291 B.I.N. Liu 58, 277 B.I.N.H. Nguyen Hoang Thai 105, 471 B.K. Kim 87, 89, 396, 404, 405 B.L. Hu 88, 401 B.L. Meena 66, 312 B.N. Naresh 65, 304 B.N. Rajeev 15, 141 B.P. BHATT 65, 105, 306, 473

38, 79, 196, 364 97, 437 67, 313

B B. Akter 43, 218 B. Balakrishna 77, 357 B. Berger 52, 250 B. BHATTACHARJEE 39, 201 B. Bui Chi 103, 464, 465 B. BUI CHI 93, 419 B. Bui Chi Buu 103, 464 B. Buu 69, 323 B. Cheah 63, 296 B. Chi Buu 69, 70, 324, 325 B. ChiBuu 93, 421 B. Collard 26, 27, 68, 81, 96, 162, 319, 371, 432 B. Cottyn 95, 428 B. Courtois 9, 26, 56, 127, 162, 270 B. COURTOIS 54, 104, 257, 467 B. Dedicova 97, 439 B. Du 6, 115 B. Gupta 69, 322 B. Kaur 20, 143 B. Kim 43, 102, 218, 458, 459 B. Kofi 26, 159 B. Kovacevik 67, 314 B. Kumari 79, 365 B. Li 32, 177 B. Liu 58, 74, 77, 100, 277, 343, 354, 452 B. Maji 49, 239 B. Manneh 26, 159 B. Marandi 7, 117 B. Marathi 10, 75, 130, 344

498

499

500

501


B.P. M Swamy B.P. Mallikarjuna B.P.M. Swamy B.P.S. Malik B.R. Raju B.U.U. Bui Chi B.W. Tonnessen Babu Brajendra

C. Li 31, 74, 172, 342 C. LI 74, 342 C. Liang 42, 211 C. Manito 45, 92, 224, 418 C. McGuire 23, 81, 152, 368 C. Moehs 95, 429 C. Nakamura 43, 216 C. NARNE 90, 410 C. Ogata 29, 166 C. Orn 83, 379 C. PERIN 44, 54, 220, 257 C. Pesce 20, 145 C. QUINONES 59, 281 C. Raghavan 18, 33, 76, 118, 179, 353 C. ramakrishna 258 C. Ramakrishna 54 C. Ranganathan 51, 247 C. SOLIS 59, 281 C. Sugiyama 96, 435 C. Sullivan 13, 135 C. Tung 55, 262 C. Uddaraju 107, 482 C. Vera Cruz 19, 20, 21, 27, 76, 77, 96, 120, 162, 350, 354, 432 C. Vera-Cruz 78, 358 C. Vijayalakshmi 60, 284 C. Xinbo 47, 232 C. Yang 102, 458, 459 C. Ye 15, 52, 54, 55, 93, 141, 251, 260, 261, 265, 422 C. Zamora 60, 101, 282, 454 C. Zhai 20, 145 C.B. Andaya 26, 159 C.B. Dela Viña 51, 245 C.C. Cardenas 46, 227 C.C. Llorente 86, 389

45, 224 71, 329 55, 92, 264, 418 81, 369 59, 278 105, 471 74, 339 294 60, 62, 286, 294

C C. Adeva C. Alarcón C. Andaya C. Basavanneyya C. Bermudez C. Billot C. Blanchard C. Boualaphanh C. Brondani C. Cantos C. Casal C. Charbonnel C. Cobo Castillo C. Cortaga C. Dalid C. Dilla-Ermita C. Dizon C. Duenas C. Duenas Jr C. Grenier C. Gunathilake C. He C. Jahn C. Lee

24, 91, 153, 415 99, 445 72, 95, 331, 430 92, 417 97, 439 9, 127 24, 156 25, 158 97, 439 45, 223 108, 486 3, 114 29, 168 67, 316 54, 259 104, 468 96, 432 44, 222 24, 153 26, 162 86, 390 97, 436 19, 120 56, 269


D. Deborah 90, 406 D. Divya 73, 336 D. Do 46, 226 D. Dwivedi 106, 107, 477, 478, 481 D. Facciotti 23, 81, 152, 368 D. Fujita11, 21, 35, 37, 38, 63, 75, 78, 131, 147, 184, 194, 195, 198, 297, 298, 348, 358, 359 D. Fuller 1, 111 D. Huang 33, 40, 179, 204 D. Johnson 59, 280 D. Kavithamani 104, 470 D. Kim 88, 397 D. Lee 43, 216 D. Loeffler 23, 81, 152, 368 D. Lozada 77, 354 D. Luo 47, 229 D. Mackill 53, 68, 253, 321 D. Malarvizhi 60, 98, 284, 443 D. Mei 33, 179 D. MIEULET 44, 104, 220, 467 D. Mishra 95, 428 D. Nurdiani 70, 325 D. Pakr 50, 244 D. Park 73, 88, 336, 397 D. Raj R 8, 125 D. Rathnasekera 100, 451 D. Ratnasekera 9, 126 D. Sanchez 68, 321 D. SHAH 59, 281 D. Shin 50, 73, 88, 244, 336, 397 D. SHOBA 66, 310 D. Silue 71, 327 D. Subrahmanyam 33, 50, 60, 62, 180, 242, 286, 294 D. Sudhakar 41, 209

C.F. Pantouw 70, 325 C.H. Balachiranjeevi 73, 337 C.H. Su 105, 470 C.H.A.U. Thanh Nha 93, 421 C.H.I. Buu bui 86, 388 C.J. Dilla 105, 472 C.J. Dilla-Ermita27, 77, 96, 101, 162, 354, 432, 454 C.J. Liu 99, 448 C.L. Diaz 31, 174 C.M. Lee 60, 285 C.M. Marfori 35, 185 C.M. Vera Cruz 71, 76, 95, 99, 329, 353, 428, 449 C.N. Neeraja33, 35, 88, 97, 180, 186, 401, 437 C.P. Moehs 23, 152 C.P. Quinones 76, 352 C.P. Ubales 47, 231 C.P.D. Rajan 101, 456 C.S. Bueno 56, 270 C.S. Wang 105, 470 C.W. Tung 56, 57, 269, 274 C.Y. Li 42, 212

D D. Abeysiriwardena D. Arreza D. BALAKRISHNAN D. Bhatia D. Bolser D. Brunel D. Burman D. Ch.V. D. Chen

61, 289 94, 427 72, 332, 333 20, 143 13, 135 9, 127 49, 239 80, 367 34, 182

500

501

502

503


D. Tabanao 78, 358 D. Tao 11, 29, 52, 82, 98, 132, 167, 249, 373, 374, 440 D. Voytas 45, 223 D. Ware 13, 135 D. Xue 3, 113 D. Zeng 3, 113 D.A. Tabanao 27, 76, 86, 163, 352, 392 D.A. Yu 76, 90, 351, 407 D.A. YU 57, 272 D.A.K. Deborah 56, 266 D.J. Kim 89, 405 D.J. Mackill 108, 486 D.K. Dwivedi 106, 107, 476, 479, 480 D.K. Sharma 49, 239 D.K. Sidde Gowda 71, 72, 329, 334 D.L.E. Waters 41, 207 D.M. Kim 36, 61, 108, 190, 290, 483 D.M. Pandey 45, 67, 223, 313 D.M.N. Dissanayake 74, 88, 341, 399 D.N. Lozada 96, 432 D.N. Singh 7, 108, 117, 484 D.P. Singh 7, 49, 117, 239 D.R. A. Ismail 57, 273 D.R. D. J. Mackill 57, 273 D.R. E. Septingsih 57, 273 D.R. H. U. Ahmed 57, 273 D.R. Huang 88, 401 D.R. S. Heurer 57, 273 D.S. Brar 20, 75, 143, 348 D.S. Kakulandara 50, 243 D.S. Park 72, 87, 331, 394 D.V.N. Chakravarthi 62, 293 D.W. Jang 51, 57, 90, 246, 272, 407

E E. Ahn 102, 458, 459 E. Angeles 37, 193 E. Arafat 108, 485 E. Ardales 74, 343 E. Brewer 44, 219 E. Ella 53, 254 E. GUIDERDONI 44, 54, 104, 220, 257, 467 E. Gutiérrez 99, 445 E. Hanzawa 99, 447 E. Hoque 48, 233 E. Huang 18, 118 E. Jeong 102, 458, 459 E. Jobet 3, 114 E. Kariali 33, 177 E. Kharitonov 35, 56, 185, 269 E. Koh 42, 43, 214, 218 E. Kokiladevi 41, 209 E. Kostadinovska 67, 314 E. Lombi 24, 153 E. Matthus 54, 259 E. Mendoza 78, 358 E. Mercado 94, 427 E. Mullins 97, 439 E. Nissila 1, 27, 101, 104, 162, 454, 468 E. Redona 18, 54, 260 E. Redoña 33, 54, 55, 179, 261, 265 E. Sanchez 68, 317 E. Septiningshih 7, 117 E. Septiningsih 46, 53, 62, 68, 226, 253, 292, 319, 321 E. Shakiba 26, 161 E. Simon 37, 63, 194, 298 E. Suiton 68, 321


E. Sulaiman 94, 426 E. Sunian 33, 178 E. Tako 24, 153 E. Tandayu 104, 468 E. Tecson-Mendoza 54, 259 E. THOMAS 54, 257 E. Yamamoto 2, 52, 98, 113, 253, 442 E. Zuniga 97, 439 E.A. Barlaan 64, 300 E.A. Siddiq 56, 90, 266, 406 E.C. Arocena 27, 163 E.C. Borresen 25, 157 E.D. Pascual 51, 245 E.D. Redoña 52, 93, 251, 422 E.E. Delorean 76, 353 E.G. Oreiro 84, 381 E.H. Vargas 94, 423 E.L.D.A. Siriwardena 50, 243 E.M. Septiningsih 48, 61, 66, 108, 233, 288, 308, 486 E.M.A.N. Fahmy 38, 198 E.M.R.D. Edirisinghe 88, 399 E.O. Espejo 86, 392 E.P. Greenberg 90, 409, 410 E.P. Ryan 25, 157 E.S. Avellanoza 86, 392 E.S. Ladia 47, 231 E.S. Nazareno 86, 392 E.S. Tandayu 77, 96, 354, 432 E.S. Zaplin 24, 156 E.U.U. Rathnathunga 31, 103, 173, 462 E.Y. Ardales 77, 354 E.Y. Danquah 96, 433

F F. Auguy 20, 145 F. Borja 78, 358 F. CISSE 95, 427 F. Danila 36, 37, 39, 44, 188, 194, 199, 200, 220 F. DIVOL 44, 220 F. Dkhar 65, 308 F. Entila 53, 254 F. Hu 8, 11, 29, 30, 82, 123, 132, 167, 170, 373 F. Jodari 26, 95, 159, 430 F. Li 85, 387 F. Lin 20, 145 F. Montecillo36, 39, 42, 44, 188, 199, 213, 220 F. Pérez de Vida 34, 99, 182, 445 F. Pontvianne 3, 114 F. Tenorio 54, 55, 260, 261, 265 F. Tesoro 45, 92, 224, 418 F. Wang 99, 448 F. White 7, 18, 117 F. Yang 52, 249 F.A. Tenorio 52, 251 F.C. De Guzman 83, 380 F.C. Nogoy 51, 76, 246, 351 F.J. Agosto-Perez 26, 161 F.J. Maathuis 61, 289 F.K. Fukai Shu 46, 228 F.M. Arines 24, 91, 153, 415 F.N. Borja 85, 385 F.P. Waing 76, 352 F.Y. Hu 42, 214

502

503

504

505


G. Prahalada 71, 329 G. Pushpalatha 60, 62, 286, 294 G. Rahayu 22, 149 G. Ravi Kumar 39, 50, 201, 242 G. Ren 47, 229 G. REN 94, 424 G. Rizal 32, 82, 85, 176, 375, 386 G. Samarasinghe 50, 243 G. Sanchez 104, 468 G. Sanghera 60, 284 G. Senanayake 90, 409, 410 G. Shiva Prasad 107, 482 G. Singh 20, 143 G. Singleton 59, 280 G. Swathi 80, 367 G. Thomas 8, 125 G. Uday 21, 148 G. Varaprasad 73, 335 G. Varghese 8, 125 G. Wang 18, 119 G. Wu 13, 135 G. Xue 68, 318 G. Yeltatzie 72, 95, 331, 430 G. Yi 94, 425 G. Zhang 3, 58, 113, 277 G. Zhu 100, 450 G.A. Atienza-Grande 77, 356 G.A. DeClerck 26, 161 G.A.F. Cariño 96, 432 G.A.U. Jayasekera 74, 341 G.B. G. Barry 86, 393 G.B. Gregorio 49, 51, 54, 55, 61, 62, 64, 104, 239, 240, 245, 259, 262, 290, 293, 295, 302, 467 G.C. Eizenga 26, 161 G.D.A. Priyantha 62, 291

G G. Abeysundara Hewawasamge 88, 398 G. Akinwale 104, 467 G. An 20, 143 G. Anuradha 90, 406 G. Atienza-Grande 78, 358 G. Barry24, 44, 45, 92, 105, 153, 222, 224, 416, 418, 474 G. BELIGAN 84, 381 G. Borja 92, 416 G. Dharwal 107, 479 G. Diaz 50, 241 G. Dimayuga 32, 84, 175, 382 G. Dong 3, 113 G. Donoso 60, 283 G. Droc 9, 127 G. DROC 104, 467 G. Grande 78, 358, 359 G. Gregorio 8, 18, 33, 50, 59, 68, 81, 125, 179, 241, 280, 320, 371 G. Haberer 30, 169 G. He 6, 115 G. Lee 20, 43, 143, 218 G. Lorenzana 36, 39, 42, 188, 199, 213 G. Malitic 27, 104, 162, 468 G. Mohammadi-Nejad 85, 388 G. Mosquera 76, 353 G. Nematzadeh 85, 388 G. Nirmala Devi 91, 412 G. Padma Vathi 91, 412 G. Padmavathi 49, 68, 239, 320 G. Padmawathi 7, 117 G. Parray 60, 284 G. PAULUZZI 44, 220


H. Koh26, 43, 44, 160, 215, 216, 217, 218, 219 H. Kumara 90, 409, 410 H. Le Huy 69, 321 H. Leung 18, 23, 33, 74, 76, 77, 103, 118, 152, 179, 339, 353, 354, 356, 463 H. Li 52, 249 H. Lin 36, 39, 188, 200 H. Liu 19, 120 H. Nakanishi 24, 155 H. Nguyen Van 103, 465 H. Park 20, 106, 143, 475 H. Parkinson 13, 135 H. Ratnayake 50, 243 H. Saito 22, 85, 102, 150, 386, 461 H. Sakakibara 41, 206 H. Sato 64, 300 H. Takagi 78, 361 H. Takehisa 102, 461 H. Thein 54, 260, 261 H. Tokairin 102, 461 H. Tsunematsu 2, 113 H. Verdeprado 75, 348 H. Wang 34, 183 H. Watanabe 65, 305, 306 H. Yaegashi 78, 361 H. Yasui 21, 29, 37, 78, 80, 147, 166, 193, 358, 360, 366 H. Zhang 40, 204 H.A. Alamele 90, 408 H.A. Pham Thi Thu 103, 465 H.B. Diwakar 59, 278 H.C. Lin 37, 39, 44, 194, 199, 220 H.H. Kim 38, 197 H.H. Le 101, 457 H.I.E.U. Nguyen Van 105, 471

G.F. War 79, 365 G.J. King 41, 207 G.J. Tiwari 24, 156 G.K. Prajapati 45, 223 G.K. Sahi 20, 143 G.M. Bueno 91, 415 G.M. Dharshini 72, 334 G.P. Verma 106, 107, 476, 477, 479, 480 G.P. Yoga 80, 367 G.S. Capilit 83, 380 G.S. Jahan 87, 393 G.S. Laha 21, 24, 73, 97, 146, 154, 335, 337, 338, 437 G.S. Varaprasad 24, 154 G.V. Vergara 64, 302 G.Z. Doblas 64, 302

H H. Adu-Dapaah 96, 433 H. Ahmed 67, 315 H. Beard 44, 219 H. Begum 81, 371 H. Doucouré 20, 145 H. El-Mowafi 38, 108, 198, 485 H. Hirabayashi 63, 64, 66, 297, 300, 311 H. Hirochika 42, 211 H. HOSSEINI MONFARED 68, 318 H. Iwata 98, 442 H. Ji 20, 143 H. Kang 47, 94, 229, 424 H. Kato 2, 113 H. Khatun 67, 315 H. Kim 20, 44, 82, 143, 219, 372 H. Kitano 36, 99, 189, 446

504

505

506

507


I. Tsutomo 15, 141 I. Wonni 20, 145 I. Yukihiro 41, 208 I. Yukimoto 44, 221 I.A. Abreu 13, 136 I.A. Rumanti 102, 462 I.DP. Gangodawatta 9, 126 I.F. Dalmacio 64, 302 I.G. Pacada 76, 84, 352, 381 I.H. Islamet-Loedin 44, 222 I.H. Kim 87, 395 I.H. Slamet-Loedin 24, 59, 77, 91, 153, 282, 356, 415 I.M.J. Mejaya 82, 375 I.P. Navea 60, 282 I.P.D. Navea 71, 329 I.R. Choi 19, 31, 71, 77, 120, 175, 329, 356 I.S. Nou 51, 57, 76, 90, 246, 272, 351, 407 I.S. Pires 13, 136 I.W. Mulsanti 102, 462

H.J. Koh 42, 60, 214, 282, 285 H.J. Lee 51, 57, 90, 246, 272, 351, 407 H.S. Balyan 62, 292 H.S. Lee 36, 61, 91, 190, 290, 411 H.S. Park 72, 331 H.T.T. Vu 43, 216 H.U. Ahmed 66, 309 H.W. Thein 52, 251 Hu H 14, 138

I I. Abdelbagi 53, 103, 254, 464, 465 I. Abdelbagi Ismail 105, 471 I. Ando 66, 311 I. Apri Rumanti 100, 450 I. Canicosa 32, 175 I. CANICOSA 84, 382 I. Choi 78, 98, 102, 358, 359, 444, 458 I. Dasgupta 74, 340 I. INDRANIL DASGUPTA 42, 211 I. Ismail 68, 318 I. Karov 67, 314 I. Lobina 33, 179 I. Mulsanti 108, 484 I. Oña 76, 350 I. Quibod 78, 358 I. Rumanti 108, 484 I. Simon-Kiss 104, 466 I. Slamet-loedin 105, 474 I. Slamet-Loedin24, 25, 36, 38, 39, 42, 44, 45, 54, 92, 188, 198, 199, 213, 220, 223, 259, 416 I. Subhakara Rao 35, 186 I. Subhakararao 33, 180 I. Takamure 40, 204

J J. AGUIRRE 84, 381 J. Ale 99, 445 J. Ali 35, 66, 67, 185, 309, 315 J. Balindong 24, 92, 153, 416 J. Beslin 41, 209 J. Borero 24, 153 J. Carandang 68, 319, 321 J. Chang 89, 404 J. Chatterjee 32, 84, 101, 175, 382, 455 J. Chen 32, 40, 177, 204 J. Chin 60, 104, 282, 468 J. Cho 73, 82, 336, 372


J. Newmah 35, 186 J. Niones 48, 234 J. Oard 74, 339 J. Oster 72, 95, 331, 430 J. Pariasca Tanaka 53, 257 J. Pariasca-Tanaka 22, 51, 54, 82, 97, 148, 248, 259, 373, 436 J. Park 106, 475 J. Pauk 104, 466 J. Pholtaisong 47, 229 J. Preece 13, 135 J. PUIG 44, 220 J. Ramos 36, 191 J. Ranada 32, 175 J. Rey 45, 92, 224, 418 J. Reyes 36, 39, 42, 188, 199, 213 J. Sáez-Vásquez 3, 114 J. Sagun 36, 37, 188, 194 J. Salonga 82, 375 J. Schoerring 25, 158 J. Shim 78, 359 J. SHIM 98, 444 J. Singh 7, 117 J. SOEWARTO 54, 257 J. Spindel 81, 96, 371, 433 J. Stangoulis 24, 153 J. Stein 13, 135 J. Stephens 74, 339 J. Suh 106, 475 J. TAILLEBOIS 34, 183 J. Tanaka 2, 113 J. Thomason 13, 135 J. Tohme 24, 26, 153, 162 J. Undan 78, 361 J. Ward 25, 158 J. Xu 3, 85, 113, 387

J. Cobb 96, 433 J. Derera 53, 255 J. Detras 85, 385 J. Dionora 37, 101, 194, 455 J. Domingo 62, 295 J. Dufey 46, 226 J. Ferdous 48, 81, 233, 369 J. Frouin 9, 127 J. Glaszmann 9, 127 J. Goncharova 35, 56, 185, 269 J. Harvey 48, 234 J. Hernandez 2, 46, 226 J. Hibberd 36, 39, 42, 44, 188, 199, 213, 220 J. Hu 3, 113 J. Itoh 40, 205, 206 J. Jairin 21, 80, 147, 366 J. Jannink 8, 124 J. JHA 59, 281 J. Kang 47, 229 J. Karthick 58, 274 J. Kim 14, 102, 137, 458 J. Kopka 25, 158 J. Krishna 15, 141 J. Kwak 87, 89, 395, 396, 404, 405 J. Lamo 53, 255 J. Lang 74, 339 J. Leach 20, 21, 25, 76, 157, 353 J. Lee 50, 51, 72, 73, 76, 78, 82, 87, 102, 106, 244, 331, 336, 358, 359, 372, 394, 395, 458, 475 J. Lewis 95, 429 J. Li 11, 29, 31, 52, 74, 82, 98, 132, 167, 172, 249, 342, 373, 374, 440 J. Lyu 8, 123 J. Mezey 8, 124

506

507

508

509


J.J. Oster 26, 159 J.J. Somera 86, 392 J.J. Unay 76, 352 J.K. Jung 26, 161 J.K. Sohn 94, 425 J.K. Tripathy 14, 139 J.L. Dwivedi 7, 117 J.L. Katara 7, 117 J.L. Xu 35, 185 J.L. Zhao 77, 354 J.M. Anguete 14, 140 J.M. J. Macabenta 86, 393 J.M. Jamago 22, 62, 151, 293 J.M. Lang 95, 428 J.M. Mitchel 46, 228 J.M. Niones 56, 268 J.M. Pasuquin 22, 150 J.M. Ramos 10, 75, 130, 347, 348 J.N. Cobb 26, 161 J.N. Reddy 7, 117 J.P. DOSSMANN 34, 183 J.Q. Undan 78, 361 J.R. Encabo 31, 77, 175, 356 J.R. Ramirez 58, 275 J.S. Bentur 73, 336 J.S. Jeong 14, 137 J.S. Lee 87, 89, 395, 396, 404, 405 J.S. Lore 20, 143 J.S. Verma 7, 117 J.S. Yumnam 65, 308 J.T. J. Tan 86, 393 J.T. Lee 94, 423 J.U. Jeung 72, 331 J.V. Sagun 44, 220 J.W. Kang 36, 61, 190, 290 J.Y. Lee 72, 87, 331, 394

J. Yang 42, 74, 212, 342, 343 J. Ying 33, 40, 179, 204 J. Yonemaru 2, 113 J. You 14, 138 J. Zhang 8, 123 J. Zhao42, 58, 82, 100, 212, 277, 373, 452 J. Zhou 11, 29, 52, 82, 98, 132, 167, 249, 373, 374, 440 J. Zhuang 33, 40, 179, 204 J.A. Egdane 65, 307 J.A. Orcino 83, 378 J.A. Tarun 66, 108, 308, 486 J.C. Amas 50, 51, 61, 241, 245, 290 J.C. Ignacio 108, 486 J.C. Ontoy 58, 275 J.C.I. Ignacio 66, 308 J.C.T. Concepcion 86, 389 J.E. Hernandez 49, 51, 55, 61, 62, 93, 240, 245, 264, 290, 295, 422 J.E. Leach 19, 71, 74, 77, 95, 120, 329, 339, 354, 428 J.E. Revilleza 35, 185 J.F. Balidion 71, 329 J.F. Balidon 19, 120 J.G. Mezey 26, 161 J.H. Chin 27, 54, 82, 96, 101, 162, 259, 373, 432, 454 J.H. Cho 72, 87, 94, 331, 394, 426 J.H. Lee 77, 87, 89, 91, 94, 356, 395, 396, 404, 405, 411, 426 J.H. Shim 77, 356 J.I. Ignacio 61, 288 J.I. Itoh 41, 206 J.I. Yonemaru 98, 442 J.I.N.G. Cai 30, 170 J.J. Mi 99, 448


J.Y. Yang J.Z. Ying Jhansi Laxmi V

K. McNally 9, 22, 23, 84, 127, 150, 152, 384 K. Miura 8, 30, 99, 123, 168, 446 K. Mohanasundaram 98, 104, 443, 470 K. Motomura 40, 202, 203 K. Nadarajah 63, 296 K. Naito 22, 150 K. Numaguchi 8, 30, 123, 168 K. Ogasawara 56, 267 K. Oshima 40, 204 K. Paramasivam 58, 274 K. Paramsivam 7, 117 K. Prabhu 10, 128 K. Pranathi 73, 87, 337, 397 K. PU 79, 364 K. R. M 61, 287 K. Rajareddy 90, 410 K. Saito 14, 140 K. Sasaki11, 36, 37, 63, 99, 131, 190, 194, 195, 297, 298, 447 K. Satoh 71, 77, 329, 356 K. Sidarath 64, 304 K. Singh 20, 68, 107, 143, 317, 480 K. Songyikhangsuthor 14, 138 K. Srinivasan 84, 384 K. Sugimoto 76, 349 K. Sujatha 21, 73, 146, 337 K. Sumanthkumar 59, 278 K. Suneetha 24, 107, 154, 482 K. Surekha 33, 88, 180, 401 K. Svyatyna 12, 132 K. Tamura 85, 386 K. Tennakoon 81, 371 K. Thiyagarajan 60, 284 K. Titong 60, 101, 104, 282, 454, 468 K. Toriyama 40, 41, 202, 203, 208

77, 354 88, 401 75, 344

K K. Acebron 32, 82, 85, 175, 176, 375, 386 K. Anuradha 62, 293 K. Anwar 14, 139 K. Aslam 99, 449 K. Bashir 24, 155 K. Chen 47, 229 K. Chickkakariappa 68, 317 K. Dartey 14, 140 K. Doi 29, 165 K. Fernando 31, 103, 173, 462 K. Fujino 46, 56, 225, 267 K. Fukuroi 40, 204 K. Furukawa 98, 442 K. FUTAKUCHI 95, 427 K. Ha 106, 475 K. Hibara 40, 206 K. Hidemi 44, 221 K. Ichinose 102, 461 K. Iftekharuddaula 48, 233 K. Igarashi 40, 202, 203 K. Ingole 79, 364 K. Ishii 98, 102, 441, 461 K. Izumi 44, 221 K. Jayaramulu 38, 196 K. Jeong 41, 207 K. Kumar 47, 97, 230, 438 K. Loke 68, 318 K. Matsubara 2, 113 K. Mayer 30, 169 K. McKenzie 95, 430

508

509

510

511


K.S. Lee K.S. McKenzie K.T. Win K.V. Mendez K.V. Prabhu Kim Kshirod K. Jena

K. Tran Dang 69, 321 K. Trijatmiko 92, 416 K. Vasantha bhanu 77, 357 K. Vinod 10, 128 K. WIN 29, 166 K. Wydra 71, 329 K. Yamamoto 96, 435 K. Yasuda 22, 85, 150, 386 K. Yoshida 8, 30, 123, 168 K. Youens-Clark 13, 135 K.A. Mosa 47, 230 K.A.C. Rasanjali 62, 291 K.B. Kempa Raju 79, 364 K.B. Kemparaju 38, 196 K.C. Bansal 30, 171 K.D. Patil 49, 239 K.G.P.B. Karunarathne31, 103, 173, 462 K.I. Hibara 41, 206 K.J. Yashavantha 105, 472 K.K. Hwu 105, 470 K.K. Jena 10, 20, 36, 63, 75, 99, 130, 143, 191, 297, 347, 348, 447 K.K. Kang 51, 57, 76, 90, 246, 272, 351, 407 K.K. Kumar 41, 209 K.K. Manohara 49, 239 K.K. Mishra 107, 480 K.K. Sahoo 69, 322 K.K. Vinod 65, 79, 304, 363 K.L. McNally 30, 83, 170, 380 K.L. Titong 96, 432 K.N. Drame 26, 82, 159, 373 K.N. Dramé 14, 140 K.O. Koyanagi 31, 175 K.R. Battan 81, 369 K.R. Trijatmiko 24, 38, 91, 153, 198, 415

91, 411 26, 159 29, 165 38, 196 65, 79, 304, 362, 363 73, 78, 102 viii, 75, 344

L L. Arul 41, 209 L. Cao 34, 182, 183 L. Cardenas 39, 200 L. Conrad 41, 210 L. CROMER 104, 467 L. Gabunada 53, 253 L. Guo 3, 40, 113, 204 L. HASSAN 49, 87, 237, 393 L. Hei 74, 343 L. Heszky 104, 466 L. Hoang 85, 387 L. Hua 20, 145 L. Huang 85, 387 L. Le 46, 228 L. Le Hung 69, 321 L. Li 30, 170 L. Liu 42, 74, 212, 342 L. LIU 74, 342 L. Nguyen thi 105, 471 L. Nguyen Thi 103, 465 L. NGUYEN THI46, 86, 93, 227, 388, 421 L. Nguyen Thi Lang 103, 464 L. Nguyen Van 69, 321 L. Nora 77, 354 L. PEREIRA 104, 467 L. Perez 83, 378


L. Poulin 20, 145 L. Romeo V 103, 464, 465 L. Romeo V Labios 105 L. Romeo V. Labios 471 L. Santos 83, 378 L. Stein 13, 135 L. Subbalakshmi 98, 443 L. Tan 68, 318 L. Torrizo 24, 91, 92, 153, 415, 416 L. Wang 20, 146 L. WILLOCQUET 84, 381 L. Xiong 14, 138 L. Zhu 3, 6, 85, 113, 115, 387 L.A.F. Lambio 93, 423 L.A.N.G. NGUYEN THI 93, 419 L.B. Madrid 46, 93, 94, 227, 423 L.B. Wu 54, 259 L.M. Pamugas 94 4 L.M. Pamugas 423 L.M. Perez 27, 31, 56, 83, 84, 163, 174, 268, 378, 381 L.P. Coloma 76, 352 L.P. Tiwari 64, 304 L.R. Vemireddy 56, 266 L.R. VEMIREDDY 90, 406 L.T. L. Torrizo 86, 393 L.U.Y. Trinh thi Luy 86, 388 L.V. Kochian 26, 161 L.V. Subba Rao 60, 62, 107, 286, 294, 482 L.V. Subbarao 53, 254

M. Acoba 36, 39, 42, 188, 199, 213 M. Alcasid 82, 375 M. Arceta 50, 241 M. Arif 99, 449 M. Ashikari 8, 30, 37, 99, 123, 168, 193, 446 M. Asilo 92, 418 M. Baek 106, 475 M. Balram 75, 99, 347, 348, 447 M. Baltazar 61, 288 M. Baraoidan 74, 339 M. Barath laxmi 77, 357 M. Becker 54, 259 M. Bernardo 94, 427 M. Bhatta 72, 334 M. Burgos 76, 350 M. Calingacion 25, 158 M. Casimero 59, 280 M. Chen 32, 177 M. Cook 12, 134 M. Datta 66, 312 M. De Ocampo 65, 307 M. Dela Rosa 39, 42, 199, 213 M. Devarajan 60, 284 M. Diaz 49, 240 M. Dingkuhn 22, 38, 150, 196 M. Dionisio-Sese 39, 92, 200, 416 M. Dionora 44, 84, 220, 382 M. Dubey 50, 244 M. Dwiyanti 27, 162 M. Edwards 41, 210 M. Ferrer 50, 241 M. Fitzgerald 25, 88, 158, 400 M. Frei 54, 259 M. Garladinne 39, 201 M. Girijarani 77, 357

M M. Ab Ghaffar M. Abou-Ellail

33, 178 3, 114

510

511

512

513


M. Nakamura 99, 446 M. Nakata-Kano 48, 234 M. Nam 50, 73, 88, 244, 336, 397 M. Narayanamma 101, 456 M. Ndjiondjop 26, 159 M. Ndomondo 48, 234 M. Nigorikawa 98, 442 M. Norouzi 36, 188 M. Norvie 94, 425 M. Obara 11, 34, 35, 84, 95, 99, 131, 181, 184, 383, 431, 447 M. Okamura 41, 42, 208, 211 M. Okazaki 40, 202 M. Oost 46, 226 M. Ouk 83, 379 M. PAL 59, 281 M. Pandey 73, 108, 335, 484 M. Paredes 60, 99, 283, 445 M. Pelayo 53, 253 M. Pichiayya pillai 92, 417 M. Prias 97, 439 M. Rahman 100, 452 M. Rai 7, 65, 79, 117, 308, 365 M. Rapolu 92, 417 M. Raveendran 72, 332 M. Reveche 104, 468 M. Riemann 12, 132 M. RIVARD 104, 467 M. Roanae 59, 279 M. Rosa 36, 188 M. Sabar 49, 99, 237, 449 M. Sakata 29, 165 M. Salam 48, 236 M. Sapasap 45, 223 M. Sarker 55, 263 M. Shakila 49, 239

M. Hashim 33, 94, 178, 426 M. Hazman 12, 132 M. He 29, 98, 167, 440 M. Hossain 91, 413 M. Hutin 20, 145 M. Ikeda 36, 189 M. Islam 8, 125 M. Jahani 85, 388 M. Jamal 33, 178 M. Jose 8, 125 M. Kangabam 86, 391 M. Keays 13, 135 M. Kim 102, 336, 458 M. Kojima 41, 206 M. Kotaro 44, 221 M. Kumar 106, 476, 477 M. Kumaraswamy 53, 254 M. Leyton 60, 283 M. Lorieux 97, 436 M. M. Saeri 100, 453 M. MacDonald 44, 219 M. Maekawa 25, 99, 157, 447 M. Malabayabas 38, 196 M. Manzanilla 91, 92, 415, 416 M. Mary Jeanie Telebanco-Yanoria 71, 328 M. Matsumura 21, 80, 147, 366 M. Maziah 88, 399 M. Mendioro 50, 92, 241, 416 M. MIMURA 40, 205 M. Mishra 92, 418 M. Mittal 84, 384 M. Mohd Yusof 94, 426 M. Mombeini 67, 315 M. Monthatong 47, 229 M. Nagarajan 10, 65, 79, 128, 304, 363


M.B. CHETTI 37, 104, 192, 469 M.B. Ines 22, 150 M.C. De Peralta 47, 231 M.C. Ferrer 31, 174 M.C. Luu 101, 457 M.C. Nino 51, 57, 76, 246, 272, 351 M.C. Yadav 64, 304 M.C.N. Banaticla-Hilario 30, 170 M.D. Asante 96, 433 M.D. Jamaloddin 80, 367 M.E. Fabreag 108, 483 M.E. Guttman 12, 134 M.E. Hoque 81, 369 M.E.B. Naredo 83, 380 M.G. Acoba 44, 220 M.G. Borja 91, 415 M.G. Diaz 39, 77, 200, 354 M.G. Gayathri 38, 196 M.G. Jee 51, 90, 246, 407 M.G. Rani 7, 117 M.G.Q. Diaz 61, 64, 290, 302 M.H. Nam 72, 87, 331, 394 M.H. Wright 26, 161 M.H.R. Nguyen 95, 428 M.I. M. Inabangan 86, 393 M.I.C. Calayugan 31, 83, 174, 378 M.J. Dionora 32, 82, 176, 375 M.J. Thomson 27, 55, 61, 64, 66, 77, 96, 97, 101, 162, 263, 288, 301, 308, 354, 432, 436, 454 M.J.A. DIONORA 32, 175 M.J.N. Fuentes 22, 151 M.K. Hossain 81, 370 M.K. K Monaco 13, 135 M.K. Kim 91, 411 M.K. Reddy 39, 201

M. Sheshu Madhav 24, 73, 154, 335 M. Sheshumadhav 38, 196 M. Sie 14, 71, 140, 327 M. SIE 95, 427 M. Sow 26, 159 M. Sreevalli Devi 61, 101, 287, 456 M. Srinivas Prasad 24, 73, 154, 335 M. Suchandranath Babu 62, 293 M. Tamaoki 10, 128 M. Tamiru 78, 361 M. Teraishi 22, 85, 150, 386 M. Tester 13, 52, 136, 250 M. Thomson 18, 49, 68, 104, 105, 240, 321, 468, 472 M. Uddin 34, 181 M. Umadevi 91, 102, 106, 415, 460, 476 M. Variar57, 63, 77, 78, 79, 105, 271, 300, 355, 361, 362, 473 M. Vijay Kumar 73, 335 M. Win 59, 280 M. Wissuwa 22, 51, 53, 54, 82, 84, 97, 148, 248, 257, 259, 373, 383, 436 M. Woo 44, 219 M. Wright 27, 162 M. Yadav 84, 384 M. Yamasaki 8, 30, 123, 168 M. Yano 2, 76, 98, 113, 349, 442 M. Yoon 106, 475 M.A. Asilo 45, 224 M.A. Fitzgerald 86, 389 M.A. Newaz 55, 263 M.A. Salam 64, 301 M.A.H. M. A. Hossain 86, 393 M.A.R. Bhuiyan 81, 370 M.A.R. Orbase 47, 231 M.A.Y. Reveche 101, 454

512

513

514

515


M.T. Rose M.T. SHARMA M.T. Sta. Cruz M.V. Arceta M.V. Mohankumar M.V. Romero M.V. Sapasap M.Y. Reveche Mauleon. R Motilal

M.L. Ballesfin 75, 347 M.L. Barry 14, 140 M.L. Raorane 41, 210 M.L.S. Jubay 61, 290 M.M. Del Valle 75, 347, 348 M.M. Islam 48, 81, 236, 369 M.M. Oliveira 13, 136 M.M. Sun 90, 407 M.P. Catolos 58, 275 M.P. de Ocampo 263 M.P. De Ocampo 55 M.P. Rajanna 59, 68, 278, 317 M.R. A. Pamplona 57, 273 M.R. Islam 64, 301, 302 M.R. Karim 48, 236 M.R. Yoon 87, 89, 395, 396, 404, 405 M.R.C. Laza 38, 196 M.R.G. Burgos 95, 428 M.R.J. Najiar 79, 365 M.R.S. D. Sanchez 57, 273 M.R.V. Priya 21, 146 M.S. Alam 87, 393 M.S. Ali 81, 369 M.S. Anantha 66, 312 M.S. Choi 42, 214 M.S. Dwiyanti 96, 432 M.S. Kim 51, 57, 246, 272 M.S. M. Samia 86, 393 M.S. Madhav 21, 73, 97, 146, 337, 437 M.S. Mendioro 51, 61, 64, 77, 245, 288, 290, 302, 354 M.S. Prasad 97, 437 M.S. Rahman 64, 301 M.S. Ratnakar 59, 278 M.S. Sheshshayee 59, 278 M.S. Tun 59, 280


51, 248 63, 299 58, 275 62, 295 59, 278 86, 392 24, 38, 153, 198 27, 96, 162, 432 23, 152 53, 254

N N. Abd Karim 33, 178 N. Ahlgren 90, 409, 410 N. Ahmadi 9, 26, 56, 127, 162, 270 N. Alexandrov 12, 23, 84, 133, 152, 384 N. Aoki 41, 42, 208, 211 N. Bandillo 18, 33, 103, 118, 179, 463 N. C N 61, 287 N. Chamundeswari 77, 357 N. Desamero 47, 231 N. Dissanayake 31, 103, 173, 462 N. Durut 3, 114 N. Elayda 82, 375 N. Fujita 25, 157 N. Hieu van 93, 421 N. Hirotsu 7, 116 N. Hoque 8, 125 N. Ishak 81, 371 N. Iwata 46, 225 N. Kanno 52, 253 N. Kitazawa 76, 349 N. Kobayashi 11, 34, 35, 37, 38, 63, 84, 131, 181, 184, 194, 195, 198, 297, 298, 383

N. Yasuda 76, 349 N. Zenna 26, 48, 159, 234 N. ZENNA 95, 427 N.A. Ansari 35, 186 N.A. Larazo 82, 375 N.A. Rohmah 82, 375 N.B. Lucob 56, 268 N.C. Ramos 86, 392 N.G. Hanamaratti 105, 472 N.G. HANAMARATTI 37, 63, 91, 104, 192, 299, 413, 414, 469 N.K. Gautam 107, 482 N.K. Nishizawa 24, 155 N.K. Saha 48, 236 N.K. Singh7, 55, 62, 65, 77, 117, 265, 292, 304, 355 N.K. Tsakirpaloglou 41, 210 N.L. Manigbas 27, 163 N.N. Baliuag 47, 231 N.N. Giao 29, 165 N.N.A. Baliuag 93, 422 N.P. Mandal 7, 66, 117, 312 N.P.S. De Silva 81, 369 N.R. Sackville Hamilton 23, 30, 83, 152, 170, 380 N.S. Kottearachchi 67, 314 N.S. Rani 73, 88, 337, 401 N.T. Quilloy 99, 447 N.V. Desamero 27, 163 Nilanjay 7, 117

N. Kottearachchi 49, 95, 238, 430 N. Kumar 55, 262 N. Lakra 14, 69, 139, 323 N. LANAU 44, 220 N. Larazo 32, 85, 176, 386 N. Mandal 57, 63, 271, 300 N. Manigbas 26, 27, 46, 93, 94, 227, 423 N. Mattes 59, 282 N. Mori 43, 85, 216, 386 N. Nobuya Kobayashi 71, 328 N. Nyo Nyo Mar 88, 399 N. Oliva 24, 105, 153, 474 N. Pandey 51, 249 N. Sailaja 33, 180 N. Sarala 68, 317 N. Sarla 60, 62, 68, 286, 294, 320 N. Sattar 100, 452 N. Shamsudin 55, 264 N. Shivakumar 71, 72, 329, 334 N. Shobha Rani 21, 24, 38, 73, 87, 97, 107, 146, 154, 196, 335, 397, 437, 482 N. Singh 10, 14, 15, 20, 26, 128, 143, 161 N. Sofi 60, 284 N. Sravan Raju 38, 196 N. Sreenivasulu 24, 25, 86, 389 N. Takano-Kai 29, 165 N. Tanaka 41, 206 N. Thai Binh 69, 323 N. Thi Lang 69, 70, 323, 324, 325 N. Tran Thi 103, 105, 464, 471 N. Tsakirpaloglou 38, 91, 198, 415 N. Usyati 80, 367 N. Van Hieu 69, 70, 324, 325 N. Vieira 104, 468 N. Yadav 7, 117 N. Yao 20, 145

O O. Adeniran O. Dhankher O. El-Badawi

514

515

516

517

90, 408 47, 230 108, 485


P. Jeyaprakash 66, 310 P. Kersey 13, 135 P. Koteshwar Rao 38, 196 P. Kumar 59, 279 P. Latha 61, 101, 287, 456 P. Lestari 26, 82, 160, 375 P. Lisdiyanti 80, 367 P. Lumanglas 36, 63, 190, 297 P. Mahadevu 72, 334 P. Malabanan 92, 416 P. Manimaran 39, 50, 201, 242 P. Manosalva 74, 339 P. MAYONNOVE 104, 467 P. Mohapatra 33, 177 P. Natarajkumar 21, 73, 146, 337 P. Nick 12, 132 P. Okori 53, 255 P. Pablico 32, 37, 176, 194 P. Pesaresi 54, 259 P. Quick 39, 44, 199, 220 P. Raghuveer Rao 53, 254 P. Raja Sekhar 101, 456 P. Ramanarao 77, 357 P. Revathi 33, 38, 79, 97, 180, 196, 364, 437 P. S.K 79, 105, 364, 472 P. Sanchez 47, 232 P. Santosh 90, 406 P. satishkumar 258 P. Satishkumar 54 P. Satyanarayana 90, 410 P. Sawain 7, 117 P. Seguttuvel 53, 254 P. Senadheera 61, 289 P. Senguttuvel 38, 79, 196, 364 P. SENGUTTUVEL 104, 469

O. KAJIHAUSA 90, 408 O. Manangkil 43, 216 O. Myint 46, 59, 226, 280 O. Omar 33, 94, 178, 426 O. SOULEYMANE 56, 267 O.E. Manangkil 27, 163 O.N. Singh 7, 39, 117, 201 O.N. SINGH 65, 105, 306, 473 O.P. Veram 49, 239 O.P. Verma 106, 107, 476, 478, 479, 480, 481 O.P. Vrema 106, 477 O.Y. Jeong 91, 411

P P. Balasubramanian 41, 209 P. BC 8, 125 P. Bhowmick 10, 128 P. Biswas 86, 393 P. Cabas 60, 283 P. Capistrano 78, 99, 358, 449 P. Chadha-Mohanty 24, 44, 45, 91, 153, 222, 223, 415 P. Chintahala 92, 417 P. Comella 3, 114 P. Cruz 46, 226 P. Cuong 37, 193 P. D’Eustachio 13, 135 P. Dharmawardhana 13, 135 P. Francisco 24, 153 P. FRANCISCO 44, 222 P. G.K.Koutu 102, 460 P. Hedayati 68, 318 P. Jaiswal 13, 135 P. Jayaramireddy 90, 410


P.S. Reddy P.S. Saha P.S. Virk P.T. Maturan P.T. Thanh P.T. Thu Ha P.V. Hemachandra P.V. P. Virk

P. Singh 10, 49, 55, 129, 265 P. Soni 14, 139 P. Sta Cruz 68, 319 P. Subudhi 10, 129 P. Sudhakar 61, 101, 287, 456 P. SURENDRA 63, 91, 299, 413 P. Tam bui 86, 388 P. Tanger 85, 385 P. Valarmathi 74, 340 P. Veerabadhiran 91, 415 P. Venkata satyanarayana 77, 357 P. Vijayalakshmi 33, 180 P. Virk 45, 81, 92, 224, 371, 418 P. Westhoff 42, 213 P. Xu 11, 29, 52, 82, 98, 132, 167, 249, 373, 374, 440 P. Yadav 71, 329 P. YOGA MEENAKSHI 66, 310 P. Yu 74, 344 P.C. Sharma 7, 117 P.C. Sta Cruz 62, 93, 295, 422 P.C. Sta. Cruz 55, 264 P.H. Goodwin 71, 329 P.J. Suryendra 38, 196 P.K. Bhowmick 65, 79, 304, 363 P.K. Singh 7, 55, 77, 97, 106, 107, 117, 262, 355, 438, 476, 477, 478, 479, 480, 481 P.K. Yadav 106, 107, 476, 479, 480 P.M. SALIMATH 37, 91, 104, 192, 413, 414, 469 P.N. Harathi 101, 456 P.R. Rao 7, 117 P.R.O.F. Chandragiri cheralu 93, 420 P.S. Biswas 91, 413 P.S. Nandanwar 75, 345

39, 201 48, 236 75, 348 58, 275 30, 168 69, 70, 324, 325 97, 436 86, 393

Q Q. Liu Q. Pan Q. Qian Q. Truong

24, 58, 77, 156, 277, 354 20, 145 3, 99, 113, 446 96, 433

R R. Abdallah 38, 108, 198, 485 R. Abdol Ali Gilani 67, 315 R. Amgai 72, 334 R. BAHUGUNA 59, 281 R. Bedre 68, 317 R. Bernardo 2, 112 R. Brendel 12, 132 R. Bryant 96, 433 R. Cabunagan 75, 78, 98, 347, 358, 359, 444 R. Chai 20, 146 R. Chen 6, 115 R. Chitra 103, 463 R. Coe 39, 101, 200, 455 R. Cooke 3, 114 R. Corral 19, 120 R. COUMARASAMY 71, 330 R. Dhakarey 12, 132

516

517

518

519


R. Ohsugi 41, 42, 208, 211 R. Oliva 78, 358 R. OLIVA 84, 381 R. Panigrahi 33, 177 R. Perraju 7, 117 R. Petryszak 13, 135 R. Piao 42, 43, 214, 217 R. Pottepalem 61, 101, 287, 456 R. Pushpam 66, 98, 102, 310, 443, 460 R. PUSHPAM 106, 476 R. Puskur 92, 417 R. Rabeson 14, 140 R. Rabindran 74, 98, 340, 443 R. RABINDRAN 72, 332, 333 R. Raj Kumar gautam 58, 276 R. Rakesh Kumar Singh 58, 276 R. Ramanathan 66, 311 R. Rangappa 68, 317 R. Rathour 77, 78, 79, 105, 355, 361, 362, 363, 473 R. RAVI KANT 42, 211 R. Reinke 69, 70, 324, 325 R. Reinkee 69, 323 R. Ronanki 56, 266 R. Russel 103, 465 R. Russel Reinke103, 105, 464, 465, 471 R. Santos 105, 472 R. Sebidos 67, 316 R. Singh 7, 117 R. Sundaram 73, 97, 337, 338, 437 R. Sunderam 92, 417 R. Sunkar 12, 135 R. Suralta 50, 241 R. SURALTA 48, 234 R. Swati Sharma 102, 460 R. Takahashi 24, 155

R. Dikkumburage 67, 314 R. Dwivedi 106, 107, 478, 481 R. Edilberto 15, 141 R. ELANCHEZHIAN 65, 105, 306, 473 R. Gain 87, 393 R. Gamuyao 54, 259 R. Gannaban 37, 63, 194, 297, 298 R. Garcia 32, 82, 85, 176, 375, 386 R. Goodman 105, 474 R. Hafeel 88, 399 R. Hall 25, 158 R. Henry 2, 24, 25, 111 R. Ikeda 64, 300 R. Ishikawa 8, 30, 83, 123, 168, 379 R. JC 8, 125 R. Joshi 68, 317 R. Karan 10, 129 R. Khare 55, 265 R. Koebnik 20, 145 R. Kumar 7, 51, 117, 249 R. Kwayu 48, 234 R. Labios 59, 69, 70, 280, 323, 324, 325 R. Laza 22, 150 R. Libiternos 37, 44, 194, 220 R. Liu 31, 175 R. Mahajan 20, 143 R. Malo 59, 282 R. Matsushima 25, 157 R. Mauleon 18, 19, 78, 84, 85, 104, 105, 118, 120, 358, 384, 385, 468, 472 R. MERCIER 104, 467 R. Mizobuchi 2, 76, 98, 113, 349, 442 R. Mogul 32, 175 R. Mohammadi 50, 241 R. Morita 98, 102, 441, 461 R. Mumm 25, 158


R.K. Chaudhary 107, 480 R.K. Ellur 79, 363 R.K. Gautam 49, 55, 97, 239, 262, 438 R.K. PEIRIS 95, 430 R.K. Sarkar 7, 39, 117, 201 R.K. Singh 18, 33, 49, 50, 55, 59, 61, 179, 239, 240, 241, 262, 280, 289 R.K. Tridjatmiko 44, 222 R.L.C. Wijesundara 74, 341 R.M. Kathiresan 7, 117 R.M. Malabanan 99, 447 R.M. Sundaram 21, 24, 38, 39, 50, 73, 79, 87, 146, 154, 196, 201, 242, 335, 364, 397 R.M. SUNDARAM 83, 376, 377 R.M.F. Cabanting 84, 381 R.P. Glahn 24, 153 R.P. Laude 64, 302 R.P. Mauleon 27, 71, 162, 329 R.P. Sharma 68, 317 R.R. Fuentes 84, 85, 384, 385 R.R. Suralta 27, 56, 163, 268 R.R. Tiwari 106, 107, 476, 479, 480 R.S. Zeigler 19, 122 R.T. Clark 26, 161 R.T. Miranda 86, 392 R.V. Cortes 22, 151 R.V. Sonti 21, 73, 146, 338 R.W. McCombie 18, 120 R.Y. Acil 61, 290

R. Tapia 36, 42, 44, 188, 213, 220 R. TAPIA 39, 199 R. Terauchi 8, 30, 123, 168 R. Thilmony 12, 134 R. unan 252 R. Unan 52 R. Velazhahan 74, 340 R. Vemula 92, 417 R. Vemuri 88, 401 R. Venuprasad 14, 140 R. Vinarao 36, 191 R. VODOUHE 95, 427 R. Wassmann 69, 70, 103, 323, 324, 325, 464 R. Wickneswari 81, 370 R. Wing 19, 22, 23, 121 R. Yadav 107, 480 R. Yasmeen 48, 233 R. Youssef 44, 219 R.A. Nepomuceno 101, 455 R.A. Reaño 83, 380 R.A.G. Saludares 62, 293 R.A.O. K. V 61, 287 R.B. Angeles-Shim 99, 447 R.B. R. Boncodin 86, 393 R.B. Vinarao 75, 99, 347, 348, 447 R.C. Cabunagan 77, 356 R.E. Zantua 65, 307 R.G. Van den Berg 30, 170 R.H. Oane 41, 210 R.H. Onae 59, 279 R.H. Wang 99, 448 R.H. Wening 102, 462 R.J. Melgar 59, 282 R.J.A. Macalalad 77, 356 R.J.R. Santos 85, 385

S S. Ahn S. AJ S. Akhtar

518

519

520

521

26, 161 105, 472 67, 315


S. Hassan 38, 198 S. Hechanova 36, 191 S. HENRY 44, 220 S. Heuer 41, 54, 67, 82, 207, 259, 313, 373 S. Hittalmani 21, 89, 148, 403 S. HITTALMANI 91, 413 S. Hsu 55, 262 S. Hulbert 74, 339 S. I 61, 287 S. Ishiguro 56, 267 S. Ishikawa 24, 155 S. Janardhan 79, 364 S. Jang 43, 218 S. Jebakumar Prince 51, 247 S. Jing 6, 115 S. JOLIVET 104, 467 S. Kadiri 60, 62, 286, 294 S. Kadkhodaei 68, 318 S. Kalyani 107, 482 S. Kamal 39, 199 S. Kambalimath 68, 317 S. KARIKATTI 63, 299 S. Karki36, 37, 39, 42, 188, 194, 199, 200, 213 S. Kawai 52, 253 S. Khatiwada 52, 251 S. Kikuchi 19, 71, 77, 120, 329, 356 S. Kim 20, 43, 60, 143, 215, 285, 359 S. Kobayashi 2, 113 S. Kogure 98, 102, 441, 461 S. Koizumi 76, 349 S. Kojima 41, 208 S. Kuchi 92, 417 S. KUCHI 61, 287 S. KUMAR 65, 105, 306, 473

S. ANURAJ 63, 299 S. Arun Kumar 38, 79, 196, 364 S. B 61, 245, 287 S. Baek 106, 475 S. Banerjee 50, 244 S. Barthakur 51, 249 S. Baufumé 20, 145 S. Begum 8, 125 S. Catausan 54, 259 S. CESARI 44, 220 S. Chen 31, 175 S. Chhikara 47, 230 S. Covshoff 36, 37, 39, 44, 188, 194, 199, 200, 220 S. Cunnac 20, 145 S. Dam Roy 55, 262 S. Deka 51, 53, 249, 256 S. Dipti 88, 400 S. Dixit 55, 58, 264, 275 S. Dossa 78, 358 S. Dow 25, 157 S. Dwivedi 106, 107, 476, 478, 481 S. Ee 68, 318 S. Eram 55, 265 S. Fox 13, 135 S. Fujii 41, 208 S. Fukuoka 52, 76, 253, 349 S. Geekiyanage 31, 90, 103, 173, 409, 410, 462 S. Geetha 41, 209 S. Ghosal 48, 233 S. Gopala Krishnan 10, 65, 78, 79, 128, 304, 361, 362 S. Gopalakrishnan 79, 363 S. Han 50, 73, 244, 336 S. Hashim 94, 426


S. Ramadevi 87, 397 S. Ray 77, 355 S. REBEIRA 95, 430 S. Robin 66, 68, 74, 90, 98, 102, 104, 106, 310, 317, 340, 408, 443, 460, 470, 476 S. ROBIN 72, 332, 333 S. Roy 30, 52, 171, 250 S. Sakhan 83, 379 S. Samonte 95, 430 S. Sanjay kumar singh 102, 460 S. satishreddy 258 S. Satishreddy 54 S. Satoto 100, 102, 450, 462 S. SAVARY 84, 381 S. Seetharaman 76, 350 S. Segami 99, 446 S. Senaweera 31, 103, 173, 462 S. SENTHIL 72, 332 S. Shamsul Amri 33, 178 S. Shuhei 44, 221 S. Sierra 75, 348 S. Singh 107, 482 S. SINGH 65, 306 S. Singla-Pareek 69, 92, 322, 323, 418 S. Sobrizal 29, 165 S. Soni 64, 303, 304 S. SONI 83, 376, 377 S. Srikanth 24, 53, 73, 87, 154, 254, 335, 397 S. SRINIVASACHARY 84, 381 S. Sultana 81, 369 S. Suresh 74, 98, 340, 443 S. Suyamto 100, 453 S. Teramoto 85, 386 S. Thakur 77, 355 S. Thein 49, 240

S. Kumari 13, 135 S. Lee 73, 336 S. Lin 57, 274 S. Listiyowati 22, 149 S. Madamsetty 92, 417 S. Madavallam 68, 317 S. MAGANTI 87, 397 S. Manonmani 60, 74, 90, 91, 98, 102, 104, 106, 284, 340, 408, 415, 443, 460, 470, 476 S. McCouch 1, 8, 19, 27, 81, 96, 97, 111, 124, 162, 371, 433, 436 S. Mesapogu 60, 62, 286, 294 S. Misman 94, 426 S. Mitrev 67, 314 S. Mouritala 14, 140 S. N 61, 287 S. Nadaradjan 58, 274 S. Nadarajan 7, 117 S. Nair 73, 336 S. Naithani 13, 135 S. Narayana Naik 89, 403 S. Natarajan 66, 311 S. Neelamraju 62, 293 S. Negrao 13, 136 S. Nethi 92, 417 S. Nugroho 70, 80, 325, 367 S. ON 8, 125 S. Pantha 52, 251 S. Park 50, 73, 88, 244, 336, 397 S. Pasternak 13, 135 S. Pathania 79, 363 S. Patil 56, 105, 266, 472 S. Qamarunnisa 48, 235 S. Rahman 24, 64, 156, 301 S. Rajeswari 98, 443

520

521

522

523


S.K.Z. Ahmed 97, 438 S.L. Carandang 96, 432 S.L. Jin 100, 450 S.L. Krishna murthy 68, 320 S.L. Krishnamurthy 7, 49, 117, 239 S.L. Singla-Pareek 14, 139 S.L. Zhang 42, 214 S.M. Balachandran 21, 73, 97, 146, 337, 437 S.M. Jang 42, 214 S.M. Quatadah 57, 271 S.M. Thein 96, 432 S.M. Yeo 108, 483 S.M. Yu 31, 172 S.M.Q. Mercado 83, 380 S.N. Ahn 36, 38, 61, 91, 108, 190, 197, 290, 411, 483 S.N. Islam 87, 393 S.N. Ryu 89, 405 S.O. Samonte 26, 159 S.P. DAS 66, 312 S.P. Singh 7, 117 S.R. Das 68, 319 S.R. Durbha 88, 401 S.R. Kim 36, 191 S.R. McCouch 26, 161 S.R. Voleti 33, 50, 53, 62, 180, 242, 254, 293 S.S. Hechanova 75, 347, 348 S.S. Jena 56, 266 S.S. SINGH 105, 473 S.T. Arold 13, 136 S.U. Byeon 76, 351 S.V. Jagadish 55, 265 S.V. Ngachan 30, 66, 171, 312 S.V.K. Jagadish 52, 59, 251, 282

S. Thirumani 49, 239 S. Thirumeni 7, 58, 117, 274 S. Tiwari 49, 84, 239, 384 S. Vanisree 80, 367 S. Verulkar 7, 50, 117, 244 S. Wanchana 32, 176 S. Wani 60, 284 S. Wei 13, 135 S. Wijesekara 49, 238 S. Yamamoto 76, 349 S. Yanagihara11, 34, 35, 84, 95, 131, 181, 184, 383, 431 S. Yanagihaya 71, 327 S. Yeo 38, 197 S. Zhang 8, 58, 74, 100, 123, 277, 343, 452 S.A. Islam 91, 413 S.A. Naveed 99, 449 S.A.H.U. Nihar 68, 320 S.E. Abdula 51, 76, 90, 246, 351, 407 S.F. Lo 31, 172 S.G.J.N. Senanayake 100, 451 S.H. Kim 60, 282 S.H. Park 51, 246 S.H. Zhang 77, 354 S.J. Han 89, 405 S.K. Chetia 7, 117 S.K. Hajira 21, 73, 87, 146, 337, 397 S.K. Offei 96, 433 S.K. Oh 87, 89, 396, 404, 405 S.K. Prashanthi 77, 355 S.K. Prashanti 78, 79, 361, 362 S.K. Sharma 7, 49, 117, 239 S.K. Sopory 69, 322 S.K. Srivastava 19, 120 S.K. Zamir Ahmed 55, 262


S.V.K. JAGADISH S.W. Kwon S.W. Yoon S.Y. Kim Satyanarayana P.V Suh J Suryanarayana Y

T. Kobayashi 80, 366 T. Kretzschmar 53, 59, 253, 282 T. Kumashiro 71, 327 T. Kwon 73, 336 T. Lafarge 56, 270 T. Li 42, 211 T. Liu 32, 177 T. Longvah 88, 401 T. Mai Van 78, 360 T. Matsumoto 8, 30, 76, 123, 168, 349 T. Mizubayashi 52, 253 T. Mohapatra 7, 10, 12, 13, 68, 117, 128, 317 T. Myint 59, 280 T. Odjo 95, 431 T. Ogawa 66, 311 T. Ookawa 96, 435 T. Pangirayi 53, 255 T. Ram 7, 35, 60, 62, 64, 68, 73, 117, 186, 286, 294, 304, 320, 337 T. Sato 61, 99, 102, 290, 447, 461 T. Sharma 10, 128 T. Shibukawa 98, 441 T. Sigari 47, 231 T. Somasekar 58, 274 T. Sonoki 98, 442 T. Takai 10, 130 T. Tanabata 98, 442 T. Tanaka 30, 98, 169, 442 T. Tanisaka 22, 85, 150, 386 T. Teophile Odjo 71, 327 T. Terao 42, 211 T. Thi Nhien 93, 421 T. Tsukiyama 22, 85, 150, 386 T. Umemoto 88, 399 T. Vanaja 65, 304

59, 281 89, 403 87, 395 87, 394 75, 344 20, 143 75, 344

T T. Abe 98, 102, 441, 461 T. Agrawal 57, 75, 271, 345, 346 T. Ahmad 7, 117 T. Ansari 67, 315 T. Anuradha 7, 117 T. Borromeo 50, 241 T. Bui Phuoc 103, 465 T. Cermak 45, 223 T. Chrismada 80, 367 T. DeLeon 10, 129 T. Fukuda 22, 51, 148, 248 T. Gideon 98, 444 T. Hansen 25, 158 T. Haque 64, 301 T. Hirasawa 96, 435 T. Hirose 41, 42, 208, 211 T. Hoang 46, 226 T. Hobo 36, 189 T. Imbe 64, 300 T. Ishii 8, 30, 83, 123, 168, 379 T. Ishimaru 11, 34, 35, 36, 37, 38, 63, 66, 84, 131, 181, 184, 190, 194, 195, 198, 297, 298, 311, 383 T. Kanbe 66, 311 T. Kazama 40, 41, 202, 203, 208 T. Kim 20, 143

522

523

524

525


T. Vo van 93, 421 T. Yamagishi 42, 211 T. Yamamoto 2, 96, 98, 113, 435, 442 T. Yang 58, 100, 277, 452 T. Zheng 85, 387 T.A.M. Bui Phuoc 105, 471 T.E. Mananghaya 83, 378 T.F. Padolina 27, 47, 83, 163, 231, 378 T.H. ANSARI 66, 309 T.H. Borromeo 55, 264 T.H. Ham 89, 405 T.H. Kim 94, 425 T.J. Rose 41, 51, 207, 248 T.L. Sage 31, 172 T.L.S. Tirimanne 61, 289 T.M. Htun 8, 30, 123, 168 T.N. SATHISHA 91, 413, 414 T.N.H. Luu 101, 457 T.R. Sharma 77, 78, 79, 105, 355, 361, 362, 473 T.S.B. T. S. Bharaj 86, 393 T.T. He 100, 450 T.T. Pham 8, 123 T.T. Tuan 20, 145 T.T.T. Nguyen 76, 349 T.V. Cao 26, 162

U. Prasadarao 54 U. Susanto 82, 375 U. Verma 106, 476, 477 U. Vineetha 101, 456 U. Yamanouchi 76, 349 U. Yeo 73, 336 U. Yoon 20, 143 U.D. Singh 77, 78, 79, 105, 355, 361, 362, 363, 472 U.D. Suh 94, 426 U.H. Yoon 51, 246 U.I.P. Perera 100, 451 U.S. Ismail 7, 117 U.S. Singh 7, 57, 75, 117, 271, 345, 346 U.W. Suharsono 22, 149

V V. Agreda 47, 231 V. Amarasinghe 13, 135 V. Andaya 72, 95, 331, 430 V. B. C 287 V. B. C. 61 V. Bartolome 77, 81, 354, 371 V. Becerra 60, 99, 283, 445 V. Bhadana 64, 303, 304 V. Dalusong 83, 378 V. Gracen 96, 433 V. Gupta 20, 143 V. Juanillas 27, 85, 104, 162, 385, 468 V. Knauf 23, 81, 95, 152, 368, 429 V. Lacorte 103, 463 V. Lella 92, 417 V. Lopena 81, 92, 371, 418 V. P 61, 287 V. Palanisamy 37, 193

U U. Gowik U. Hwang U. Hyun U. Kumar U. Makarla U. Musofa U. prasadarao

42, 213 50, 244 102, 458, 459 38, 196 68, 317 70, 325 258


V. Rai 60, 62, 286, 292, 294 V. Ramaiah 26, 159 V. Ravichandran 98, 443 V. Ravindra Babu 87, 91, 397, 412 V. RAVINDRA BABU 91, 414 V. Roja 90, 406 V. S. R 61, 287 V. Sangtong 93, 421 V. Srinivasarao 90, 410 V. Sundaresan 41, 210 V. Thakur 32, 176 V. Vakeswaran 37, 193 V. Vengadessan 58, 274 V. Verdier 19, 20, 76, 95, 120, 145, 353, 428 V. Vighneswaran 59, 279 V. Vishnu Prasanth 62, 293 V. Yadav 64, 84, 303, 384 V.A. V. Aldemita 86, 393 V.C. Andaya 26, 159 V.D. Daygon 25, 86, 158, 389 V.G. Dalusong 84, 381 V.G.N. Tripura Venkata 62, 293 V.J. Ulat 85, 105, 385, 472 V.K. Mishra 49, 107, 239, 480 V.K. Yadav 64, 83, 304, 376, 377 V.L. V. Lacorte 86, 393 V.M. Butardo 24, 156 V.M. Juanillas 84, 105, 384, 472 V.N. Singh 7, 117 V.P. Bhadana 83, 376, 377 V.R. Mangu 68, 317

W W. Deng 11, 29, 82, 98, 132, 167, 373, 440 W. Ha 98, 444 W. He 8, 123 W. Hurtada 88, 400 W. Hwang 73, 88, 336, 397 W. Kavita 91, 412 W. Kim 106, 475 W. Liu 20, 146 W. Pattanagul 47, 229 W. Quick 36, 42, 45, 101, 188, 213, 223, 455 W. Ratnam 63, 296 W. Reiner 103, 465 W. RERKSIRI 47, 232 W. Sakamoto 25, 157 W. Tyagi 65, 79, 308, 365 W. Wang 8, 85, 123, 387 W.A.D. Jayawardana 74, 341 W.D.I.P. Wimalasena 50, 243 W.E.N. Wang 30, 170 W.G. Chaminda Lal Kumara 97, 436 W.H. LI 100, 450 W.H. Ra 89, 403 W.L.G. Samarasinghe 62, 67, 291, 314 W.P. Quick 6, 31, 32, 37, 39, 82, 84, 85, 115, 172, 175, 176, 194, 200, 375, 382, 386 W.Y. Song 91, 411

X X. Deng 11, 29, 82, 98, 132, 167, 373, 440 X. Duong 101, 454

524

525

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527


X. He X. Hu X. Wang X. Xu X. Yin X. Zhang X. Zhu X.A. Tran thi Thanh X.I.N. Li X.I.N. Liu X.L. Tan X.Q. Wang X.U.N. Xu X.Y. Zhu

Y. Jia 31, 172 Y. Jiao 13, 135 Y. Kato 68, 319 Y. Kawagoe 25, 157 Y. Kim 20, 143 Y. Kishima 31, 40, 56, 77, 175, 204, 267, 356 Y. Kitomi 41, 206 Y. Koide 71, 95, 327, 431 Y. Kumar 106, 107, 476, 479 Y. Lee 42, 60, 214, 285 Y. Liao 32, 100, 177, 452 Y. Masahiro 44, 221 Y. Nagamura 98, 441 Y. Nagato 40, 41, 205, 206 Y. Okumoto 22, 85, 102, 150, 386, 461 Y. Ospina 26, 162 Y. Rahayuningsih 80, 367 Y. Raj 95, 428 Y. Sato 56, 267 Y. Singh 7, 117 Y. Song 73, 336 Y. Sun 85, 387 Y. Suraynarayana 7, 117 Y. Suryanarayana 90, 101, 410, 456 Y. Takemoto 66, 311 Y. Takeuchi 66, 311 Y. Tunginba Singh 73, 336 Y. Uga 6, 52, 99, 115, 253, 447 Y. Uma devi 68, 320 Y. Venkateswara Rao 62, 293 Y. Widyastuti 100, 450 Y. Won 102, 106, 458, 459, 475 Y. Xin 35, 187 Y. Yamagata 29, 165, 166 Y. Yang 11, 29, 82, 98, 132, 167, 373, 440

100, 452 3, 113 58, 100, 277, 452 34, 183 39, 45, 199, 223 32, 177 74, 343 86, 388 30, 170 30, 170 100, 450 89, 403 30, 170 77, 354

Y Y. Adachi 65, 305, 306 Y. AGNOUN 95, 427 Y. Aung 89, 402 Y. Chen 40, 100, 204, 452 Y. Cheng 100, 452 Y. Cho 57, 76, 106, 272, 351, 475 Y. DU 74, 342 Y. Fan 33, 40, 179, 204 Y. Fujishiro 36, 189 Y. Fujita 76, 349 Y. Fukuta11, 20, 34, 35, 37, 53, 63, 71, 74, 84, 95, 99, 131, 144, 181, 184, 195, 257, 297, 327, 328, 342, 383, 431, 447 Y. Hayashi 102, 461 Y. INUKAI 48, 234 Y. Ishimaru 24, 155 Y. Ito 98, 442 Y. Iwasaki 446 Y. Iwasaki (Japan) 99


Y.S. Kim Y.S. Lee Y.T. Yun Y.Y. Fan

Y. Yash Pal Singh 58, 276 Y. Yohei Koide 71, 328 Y. Youn 73, 336 Y. Yu 43, 52, 54, 55, 82, 216, 251, 261, 265, 373, 374 Y. Zhang 11, 20, 29, 30, 34, 52, 82, 98, 132, 145, 167, 170, 182, 183, 249, 373, 374, 440 Y. Zhu 33, 40, 74, 179, 204, 342 Y. ZHU 74, 342 Y.A. Jeon 91, 411 Y.A.N.G. Dong 30, 170 Y.A.P.K. Dayasena 62, 291 Y.B. Sohn 72, 87, 331, 394 Y.C. Kim 89, 403 Y.C. Song 72, 87, 94, 331, 394, 426 Y.G. Cho 51, 90, 246, 407 Y.H. Cho 89, 403 Y.J. Hur 72, 87, 331, 394 Y.J. Jung 90, 407 Y.J. Park 89, 403 Y.J. Won 91, 411 Y.J. Zhu 88, 401 Y.L. Du 42, 212 Y.M. Gao 35, 185 Y.O.Y.E. Yu 54, 260 Y.P. Singh 239

14, 137 89, 403 38, 197 88, 401

Z Z. Chen 100, 452 Z. Gao 3, 113 Z. Gonzaga 68, 319, 321 Z. Guo 14, 138 Z. Hua 99, 448 Z. Huang 58, 277 Z. Hussain 33, 178 Z. Jewel 96, 434 Z. Li 24, 85, 156, 387 Z. Liu 20, 145 Z. SHI 74, 342 Z. Sulaiman 81, 371 Z. Wu 11, 29, 82, 98, 132, 167, 373, 440 Z. Xianwen 47, 232 Z. Zainal 68, 318 Z. Zhang 35, 187 Z.H. Sun 100, 450 Z.I. Seraj 64, 301 Z.K. Li 35, 185 Z.R. Moni 48, 233

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