Fluorescence Microscopy Filters catalog - Horiba

Filters for Fluorescence and Life Science Instrumentation

Catalog 2009

Omega Optical celebrates 40 years! Omega Optical has been designing and producing optical filters for fluorescence microscopy and instrumentation longer than any other optical filter company. We are grateful for the advancements in science, technology and outer space made with our products. Any project, challenging or simple, we are happy to assist. Our guiding philosophy has always been to find solutions. Optical filters come in many levels of performance, and are produced using various technologies. Omega Optical has 40 years of experience working with laboratories and instrument designers to achieve the required performance. Along with years of experience, we bring a corporate commitment to cooperatively explore, understand and ultimately refine the solution. We have a strong staff of scientists from the biological field, as well as in chemistry and physics that is essential to gain the highest quality of remote analysis. While this catalog mainly represents filters for fluorescence applications, we also manufacture filters for the following: * Medical Diagnostics: Point-of-Care Instrumentation, Hematology, Macular Degeneration, Real-Time Patient Monitoring and others. * Industrial: Environmental Monitoring, Machine Vision Systems, Spectroscopy, Lasers, Color Correction, Source Leveling and others. *D efense, Aerospace and Astronomy: Infra-Red Vision Systems, Targeting Systems, LIDAR , Conflict / Weapon Simulators, Outer Space Based Instrumentation, Ground-Based Telescope Systems, and others. The entire Omega Optical staff, from the Applications Engineering team, to the Sales team, to the Manufacturing teams are anxious to work with you. Our aim is to assure that the most appropriate solution will be provided with the highest quality product.

Pricing We value our relationship with you, our customer. We will strive to produce the best product at the most competitive price, and meet your critical need for performance, quality, and service. While pricing guidelines are reflected on our website, please contact us for best pricing.

Warranty – 3 Years All of the products listed are backed by a MINIMUM 3-year satisfaction warranty. Please note that liability does not include obvious misuse of the product and is limited to repair or replacement of the product purchased. Omega Optical accepts no liability for consequential or incidental damage caused by use of the product.

How to find the right filter inside this catalog Stock and Standard Filters: These are arranged by fluorophore and emission wavelength on pages 12-18. By filter set number: Please refer to Filter Set Reference Table on page 19. By center wavelength (CWL) and cut-on and cut-off: Select from a list of excitation and emission filters, and dichroic beamsplitters on pages 20-21 and 3rd millennium 22-23. By fluorophore: Please see Fluorophore Reference Table on pages 24-26. By application: Fluorescent Proteins (pages 27-28), FRET (page 29), Qdot™ (pages 32-33), M-FISH (page 36), Ratio Imaging (page 37), Multi-band (page 38), Multi-color Imaging (pages 34-35), Real-time Imaging (page 37), Flow Cytometry (pages 43-45).

2

For current product listings, specifications, and pricing: www.glenspectra.co.uk • [email protected] • +44 020 8204 9517

Introduction

General Info . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Technology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Filter Terminology . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Filters For Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 Filter Descriptions & Nomenclature . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Choosing the Optimal Filter or Filter Set . . . . . . . . . . . . . . . . . . . . . . . . . 8 Typical Epi-fluoresence Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Frequently Asked Questions and Answers . . . . . . . . . . . . . . . . . . . . . . . . 10-11

Products

ga

Op

ti

ca

l

Appendix

Light Sources and Detectors. . . . . . . . . . . . . . . . . . . . . . . . . 46 Build-a-Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Curv-o-matic . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 How to order . . . . . . . . . . . . . . . . . . . . . 47

Ome

table of contents

Stock Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12-13 QuantaMAX Filters and Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 Quanta-Vivid (Q-Vivid) Filters and Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 ALPHA Vivid Filters and Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 Standard Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14-18 Filter Sets Reference Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Excitation Filters by Center Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 Dichroic Mirrors by Cut-On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20-21 Emission Filters by Center Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 3rd Millennium Filters by Cut-On and/or by Cut-Off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22-23 Fluorophore and Best Set Reference Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24-26 Fluorescent Protein Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27-28 Fluorescent Protein Multi-band Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 FRET Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Optimizing Filter Sets for FRET Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30-31 Quantum Dot Filters and Filter Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32-33 Pinkel Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34-35 Sedat Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 Photoswitchable Protein Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35 M-FISH Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36 Ratio Imaging Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Multi-band Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 Emission Color Chart for Multi-band Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 Caged Compound Sets. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Multi-Photon Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Beamsplitters & Mirrors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 Neutral Density Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40 IR Blocking Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 IR-DIC Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Polarizing Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Fluorescence Reference Slides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Microscope Cubes and Filter Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 Flow Cytometry Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43-45

General Information Filter Types and Technology QuantaMAX: Omega Optical’s QuantaMAX filters are manufactured using state-of-the-art Energetic Process coating technology for high durability, and low temperature sensitivity. These high throughput filters provide excellent results in all applications. They are especially effective in applications where photons are scarce and exposure times are critical. QuantaMAX filter designs consider not just peak filter transmission and fluorophore probability curves, but also optimized band placement in relation to light sources, and blocking in relation to detector sensitivity. ALPHA Technology: Omega Optical’s proprietary coating process produces extremely steep slopes and precise edge location of cut-on and cut-off wavelengths. In addition, it provides the ability to place transmission and rejection regions exceptionally close together, and high attenuation between the passband and the rejection band. 3rd Millennium Technology: The most critical feature of filter design is the transition from blocking to transmission. Precise location of band edges, coupled with steep slopes, allows for optimal transmission of spectral energy. 3rd Millennium filters are specified by the critical cut-on and cut-off edges, resulting in much more accurate band placement and bandwidth, compared to industry standard filters that are defined by a Center Wavelength (CWL) and a Bandwidth (FWHM). Multi-band Filters: Omega Optical is a co-holder of the patent on all multi-band filters; including dual-band, tripleband, and quad-band designs. Others: Bandpass Filters Edge Filters Dichroic Mirrors Beamsplitters

Full Mirrors Neutral Density Filters Rejection Band Filters Color Correction Filters

Anti-Reflective Coatings Longpass Filters Shortpass Filters Hot/Cold Mirrors

Wideband Filters Multi-band Filters Narrow-band Filters

Custom Filters – OEM Production Quantities For optimum instrument system performance we recommend collaborative engineering for custom filter solutions. Omega Optical is a leading supplier for fluorescence based instruments and applications including: Microplate Readers, DNA Sequencers & Analyzers; Gene Chip Readers; Flow Cytometers and Cell Sorters; Real Time PCR Analyzers; Scanners and Imaging Systems; High-Throughput and High-Content Systems; Fluorescence Microscopes, and others.

Custom Filters – Small Quantities Custom filters in single or small quantities are available to ship in 5 – 10 days from our extensive inventory of components. Visit Build-a-Filter at www.omegafilters.com

Aftermarket filters If you are in need of a filter that did not come with your original equipment, please go to our Build-A-Filter tool on our website. Select your instrument, tell us your filter wave length, or cut-on or cut-off, and we do the rest. No need to specify physical dimensions. See page 47.

4

For current product listings, specifications, and pricing: www.glenspectra.co.uk . [email protected] . +44 020 8204 9517

Omega Optical employs the use of 23 active coating chambers and has always utilized a wide variety of coating technologies for complex high-phase thickness thin films. We utilize Physical Vapor Deposition (PVD) with resistive element heating, Ion assisted e-Beam, and Dual Magnetron Reactive Ion Beam Sputtering; the world’s most advanced sputtering technology. No single technology is ideal for every application. As a world-class manufacturer, Omega Optical knows the attributes and limitations of each and utilizes multiple technologies to achieve best results. In addition, we consider the following to be important considerations when recommending or designing the filter that’s right for your instrument development: angle of incidence, polarization, substrate material, system speed, and temperature effects. We have our own In-House Optical Polishing, Grinding & Configuring and can supply filters in physical dimensions of 5mm – 215mm, Round, Square, Rectangular Standard Dimension Tolerances +0/-0.2mm Custom / Non-Standard Configurations Available

Terminology Angle of Incidence - the principle wavelength of all types of interference filters decreases as AOI increased. Bandwidth or Full Width Half Max (FWHM) – the spectral distance between the cut-on & cut-off wavelengths of a bandpass filter. Blocking – a measure of how well a filter stops energy of a particular wavelength from passing through. Blocking, or rejection, is expressed in optical density. An increase of 1 optical density represents a factor of 10 decrease in transmission. Center Wavelength (CWL) – the wavelength half way between the cut-on & cut-off wavelengths of a bandpass filter. Cut-Off Wavelength – the point on the falling edge of a shortpass filter or the red edge of a bandpass filter where the transmission drops to 50% of its peak value. Cut-On Wavelength – the point on the rising edge of a longpass filter or the blue edge of a bandpass filter where the transmission reaches 50% of its peak value. Polarization - at non-normal AOI, an interference filter's spectral performance in P-polarized light will differ from its performance in S-polarized light. Reflection – for filters that redirect a portion of the spectrum, such as a dichroic, this is the fraction of energy incident upon the filter at any particular wavelength that is reflected off. Substrate Material Considerations - Fused Silica, Sapphire, Silicon, Float Glass, Absorptive Glass System Speed - when filtering a converging rather than collimated beam of light, the spectrum results from the integration of the rays at all of the angles within the cone. The peak wavelength shifts about one-half the value that it would shift in collimated light at the cone's most off angle. Temperature Effects - the performance of an interference filter shifts with temperature changes due to the expansion and contraction of the coating materials. Transmission – the fraction of energy incident upon the filter at any particular wavelength that passes through the filter. Expressed as either a percent (85%) or a fraction of 1 (0.85)

For current product listings, specifications, and pricing: www.glenspectra.co.uk . [email protected] +44 020 8204 9517

5

Filters For Microscopy Filter Set General Features

• Designed for overall optimization of signal-to-noise, brightness, and multi-color discrimination. • Excitation & emission bands optimized for color perception. • Holders/cubes available to fit most microscopes.

Excitation Filter Features

• Bandpass placement maximizes excitation energy while minimizing emission band crossover. • Blocking from UV to the limits of CCD detection (1050nm) to minimize background; >/= OD5 to >/= OD6. • Blocking design in some filters significantly increases filter life by reducing transmission where not needed. • Designed for extreme heat tolerance. • High-phase thickness coatings for superior spectral control. • Not recommended for use as an emission filter as design differences may negatively effect image resolution.

Dichroic Beamsplitter Features

• Steep cut-on slopes optimize excitation and emission performance while maximizing signal. • Anti-reflection coatings to increase transmission and enhance image sharpness. • Fused silica substrates for reduced auto-fluorescence. • Substrates: optically polished for imaging applications.

Emission Filter Features

• Bandpass/longpass placement maximizes emission signal while minimizing excitation band crossover. • Anti-reflection coatings to increase transmission. • Standard thickness to minimize registration shift when switching filter sets. • Blocking design optimized to maximize emission signal. • Not recommended for use as an excitation filter when using a wide spectrum light source (as in a Mercury Burner)due to incomplete blocking across the full range of the source. For sources such as lasers and some LEDs emission filters may perform adequately as excitation.

Zero Pixel Shift ZPS is recommended for multi-color applications and results in better discrimination. ZPS is also recommended for applications such as FISH (fluorescence in-situ hybridization), CGH (comparative genomic hybridization), SKY (spectral karyotyping), and co-localization studies. Please specify when ZPS is required. An additional charge is added to the price of some sets.

6


Filter Descriptions & Nomenclature Excitation & Emission Filters

Center Wavelength Filter Design FWHM/Bandwidth, or Cut On/Cut Off 550XX50

Note: FWHM or Full Width Half Max is defined as the bandwidth at 50% of the maximum transmission of the filter.

Filter Design Bandpass filters vary in the steepness of their edges and the depth of the blocking outside the passband. • QMAX: Surface coated single substrate designs give steep edges, very high transmission and minimal registration shifts. • 3RD: 3RD Millennium Filter designs are manufactured using proprietary ALPHA technology and Omega’s patented hermetic assembly. • AF: ALPHA Filter designs are manufactured using Omega’s proprietary technology resulting in extremely steep edges, precise edge placement, and theoretical attenuation >OD 10. • DF: Discriminating Filter designs have 6 or more interfering cavities, resulting in a bandpass filter with rectangular shape, very steep edges, and very deep blocking up to Optical Density (OD) 6 outside the passband. • WB: Wide-Band Filter designs are 4 & 5 cavity designs with FWHM greater than 30nm and up to several hundred nanometers. • NB: Narrow-Band Filter designs are 2-cavity designs with FWHM typically between 0.2 and 8nm.

Dichroic Beamsplitters

Cut-On Wavelength

Filter Design

550DRLP Note: Cut-On or cut-off wavelength is defined as the wavelength at which the dichroic is at 50% of its maximum transmission value. While defined by the cut-on or cut-off wavelength, the wavelength ranges of the reflection and transmission bands are more critical to dichroic performance.

Filter Design Dichroics are used at non-normal angles of incidence (typically 45 degrees), highly reflecting one specified spectral region while optimally transmitting another. • LP: Longpass designs transmit wavelengths longer than the cut-on and reflect a range of wavelengths shorter than the cut-on. • SP: Shortpass designs transmit wavelengths shorter than the cut-off and reflect a range of wavelengths longer than the cut-off. • DC: Dichroic designs provide wide regions of both transmission and reflection, exhibiting a high degree of polarization along with a somewhat shallow transition slope. • DR: Dichroic Reflector designs provide a steep transition slope, low polarization, a wide region of transmission, and a limited region of reflection. • DCXR: Dichroic designs that provide extended reflection regions.

Miscellaneous • DB: Dual-band filter designs have two passbands and two rejection bands. • TB: Triple-band filter designs have three passbands and three rejection bands. • AR: Anti-reflection coatings are used on many filters to increase transmission and reduce back reflections.


7

Choosing the Optimal Filter Set Fluorophore(s) • Consult our Fluorophore Reference Table (pages 24-26) or the Curv-o-matic on our website for excitation and emission spectra as well as recommended filter sets. Application • What is most important in your application—bright signal, dark background, color discrimination, or high signal-to-noise? While no one design can optimize for all dimensions, many designs provide a solution that gives good overall performance. • Does your application require customized reflection and transmission specifications for your dichroic? Call us for assistance. • Does your application require “imaging quality” filters? All 3rd Millennium and stock/standard filters in this catalog are suitable for all imaging applications. Light Source • What is your light source— halogen, laser, LED, mercury, xenon? Filters are designed to optimize performance for different light sources. Detector • Specify your detector—CCD, PMT, CMOS, film, eye. Filter blocking strategies are designed to optimize performance for different detectors.

Curv-o-matic A web-based interactive database of standard filter set and component spectral curves, along with fluorophore absorption and emission spectra.

8

• Click Curv-o-matic at www.omegafilters.com • Choose a fluorophore and view spectral curve • Choose recommended filter set/component; spectral curve is overlaid; download ASCII data • Order online


Typical Epi-fluoresence Configuration Successful fluorescence imaging requires three filters mounted as a single unit in a filter cube or holder, secured in a fluorescence microscope with the proper lightsource and detector. The excitation filter, positioned normal to the incident light, has a bandpass design that transmits the wavelengths. The filtered excitation light reflects off a long-pass dichroic mirror placed at 45°and excites the fluorophore. The mirror has the unique ability to reflect more than 90 percent of the light within the reflection band while passing more than 90 percent of the light in the transmission region. This directs excitation light and fluorescence emission appropriately within the optical setup. Following excitation, the fluorophore emits radiation at some longer wavelength, which passes through the dichroic mirror and emission filter into a detector. The emission filter blocks all excitation light and transmits the desired fluorescence to produce a quality image with high signal-to-noise ratio (See below).

Detector Emission Filter

Dichroic Mirror Light Source

Excitation Filter Light Path Excitation

Light Path Emission Sample

In a fluorescence filter cube, the incident light passes through the excitation filter. The filtered light reflects off a dichroic mirror, striking the fluorophore. The longer-wavelength fluorescence emission passes through the dichroic mirror and emission filter to the detector. The emission filter blocks stray excitation light, providing bright fluorescence against a dark background.


9

Frequently Asked Questions and Answers Q: What’s a safe Angle of Incidence (AOI) range for an interference filter? A: AOI is a critical parameter to consider when purchasing an interference filter. The primary effect of an increase in the AOI on an interference coating is a shift in spectral performance toward shorter wavelengths. That is, the principle wavelength of the filter decreases as the AOI increases. A typical interference filter will exhibit only minor changes in performance with a tilt of up to 10°. However, for certain narrow band filters and transition edges of dichroics, this slight shift may cause dramatic performance changes. For advice on how tilt affects performance please call one of our engineers. Q: Would a dichroic have better reflection/transmission in S or P? A: Simply put, reflection is better in S polarized light and transmission is better in P. This characteristic is most pronounced at the transition edge, where the dichroic is going from high reflection to high transmission. Q: Why are blocking specs so critical? A: Most people know where their signal of interest lies, but sometimes do not consider potential sources of “noise”. This “noise” could be autofluorescence from the sample, or the signal from another fluorophore, or even energy from their light source. Blocking is the feature of a filter that attenuates this unwanted energy and permits the energy from the signal of interest to pass through. Using filters designed to block unwanted signals can improve signal-tonoise and robustness of the data. Q: I want sharp edges, are the 3rd Millennium filters suitable? A: 3RD M filters are manufactured using Omega Optical's ALPHA technology, which produces very steep edges, capable of handling most application needs. Standard 3RD M filters utilize an ALPHA Gamma edge that has a 3% slope factor. This means that the filter’s cut-on or cut-off edge will go from 50% peak height to OD 5 by the value: 50% peak height wavelength x (0.03). 3RD M filters can also be manufactured with an ALPHA Epsilon edge. This filter has a 1% edge factor and thus will go from 50% peak height to OD 5 by the value: 50% peak height wavelength x (0.01). Q: Do my excitation and emission filters need to transmit at the peaks of a fluorophore’s absorption/ emission probability curve? A: Not necessarily. Although it is usually best to encompass as much of the probability peaks of a fluorophore as possible, sometimes other limiting factors preclude this solution. One example is a sample with multiple labels that have significant overlap of their emission peaks. In this case, moving the emission filter off the longer wavelength fluorophore’s emission peak can improve signal discrimination.

10


Q: How do I clean filters? A: If dust and debris are the primary contaminants, filters can usually be sufficiently cleaned by using dry air (such as a puff from a pipet bulb) or compressed air. If the filters have oily substances that cannot be easily removed, either acetone or isopropanol can be used using a soft, lint free applicator, such as a Q-Tip or soft lens paper. Q: What does the arrow on the side of a filter indicate? A: Omega Optical filters should be oriented with the arrow pointing in the direction of the light path. In other words, the arrow points away from the light source and towards the detector. Q: Can I use an excitation filter as an emitter and vice versa? A: Due to different design strategies used to optimize the performance of a filter as an excitation or an emission filter, it is not recommended to use one in place of the other. Q: Can I use a dichroic from my microscope in a flow cytometer for the same dye? A: Generally, no. Flow cytometers are designed to use dichroic beamsplitters which have different specifications than a fluorescence microscopy dichroic beamsplitter. When inquiring about a particular dichroic not sold as part of a filter set, you should always specify its desired application. Q: I’m using filter set to image Cy5®, but I don’t see any image on the screen and I know I have enough dye loaded. Is the filter working properly? A: Probably, yes. Cy5® is a fluorophore which emits at the far end of the visible spectrum (peak at 670nm), this can make viewing it through the eyepiece of the microscope very difficult and typically a B/W CCD camera or PMT is needed to detect it. Many CCD cameras come with IR blocking filters housed in front of the chip and attenuate light from 650nm upwards. This effectively blocks signal from Cy5® and similar dyes from reaching the detector. Consult your camera’s manual to see if the filter can be switched off line or removed.


11

Products

Stock Filters

are available in 25 mm round for excitation and emission filters, and 25.7x36 mm rectangular for dichroic beamsplitters.

Ships within 24 hours!

QuantaMAX™ filters and sets 

Application

Low photon count fluorescence, confocal, multi-photon microscopy, live cell imaging.

Design Features

QuantaMAX™ filters are designed with consideration of peak filter transmission, fluorophore probability curves, optimized band placement in relation to light sources, and blocking in relation to detector sensitivity. Zero Pixel Shift is a standard feature in these high performance filter sets.

Stock Filters – QuantaMAX Fluorophores

Filter sets are arranged by fluorophores and emission wavelength.

Filter Set #

Applications

Components Type

Part #

Description

Exciter Dichroic Emitter

QMAX/EX QMAX/DI QMAX/EM

355-405 410LP 420-480

eGFP, Alexa Fluor® 488, Cy2®, QMAX-Green FITC



450-490 500LP 510-560

DsRed 2, Alexa Fluor® 546, 555 QMAX-Yellow Emission provides a yellow-orange color to Ds Red2, Cy3®, Rhodamine 2, TRITC TRITC and others.



510-560 560LP 570-600

Hc Red, Alexa Fluor® 568, 594 QMAX-Red Red emission and good discrimination from eGFP Mito-Tracker Red in co-expression systems.



530-570 580LP 600-650

Alexa Fluor® 647, Cy5® QMAX-FarRed



620-650 660LP 670-750

BFP, Alexa Fluor® 350, DAPI, QMAX-Blue Hoescsht 33342 & 33258

Wide exciter filter may cause cellular damage in live cell applications. Please inquire about narrow band excitation to reduce phototoxicity.

QMAX Far Red Set for Cy5 and similar dyes 100 – 90 – 80 – 70 – Transmission (%)



60 – 50 – 40 – 30 –

QMAX Far Red Excitation

20 –

QMAX Far Red Dichroic

10 –

QMAX Far Red Emission

0– 540

560

580

600

620

640

660

680

700

720

740

760

780

Wavelength (nm)

Stock Filters – QuantaMAX Dual Band Fluorophores

Filter Set #


Applications

FITC, GFP, Emerald, Turbo GFP QMAX-FITC/TRITC Dual bandpass design for multiple labeling. TRITC, DsRed, HcRed, J Red NEW!

12

Components Type

Part #

Description


QMAX/DBEX QMAX/DBDR QMAX/DBEM

485-560 485-560 520-610


Zero Pixel Shift (ZPS™)

Pricing

All single band filter sets are available with Zero Pixel Shift. Please contact one of our sales representatives for assistance.

We provide the most competitive prices. For current product pricing, please visit www.glenspectra.co.uk or contact us at:  

NEW!


Stock Filters – Quanta-Vivid Fluorophores

Filter Set #

Quanta-Vivid (Q-Vivid) filters and sets

Components Type

Part #

Description

CFP XF401 NEW!

Exciter Dichroic Emittter

XF1402 XF2034 XF3401

440QV21 455DRLP 480QV30

TRITC XF402 NEW!

Exciter Dichroic Emittter

XF1403 XF2017 XF3402

525QV45 560DRLP 645QV75

Omega Optical continues to improve its fluorescence filter line with the introduction of NEW! Quanta-Vivid filters and sets. Fluorophore specific, with very steep edges for encompassing the excitation and emission maximums of today's most popular fluorophores, and along with superb transmission in the passbands, allows for high throughput and maximizes photon capture. These filters are produced on fused silica substrates for ultimate image quality and registration, along with zero auto-fluorescence.

Alpha Vivid filters and sets 

Application

Best for most imaging applications.



Non Standard Sizes available Call for pricing.

[email protected] +44 020 8204 9517

Design Features

ALPHA Technology excitation and emission filters provide steep edges, increased blocking, and reduced auto-fluorescence.

Stock Filters – Alpha Vivid Fluorophores

Filter Set #

Filter sets are arranged by fluorophores and emission wavelength. Applications

Components

Type

Part #

Components Description

Type

Part #

Description

DAPI XF06 Optimized for Hg lamp. Hoechst 33342 & 33258 AMCA/AMCA-X


XF1005 XF2001 XF3002

365WB50 400DCLP 450AF65

CFP XF114-2 SpectrumAqua®


XF1071 XF2034 XF3075

440AF21 455DRLP 480AF30

eGFP, Cy2® XF115-2 Long pass emission filter may show more Fluorescein (FITC) auto-fluorescence. Alexa Fluor® 488


XF1073 XF2010 XF3086

475AF40 505DRLP 510ALP

eGFP XF116-2 Fluorescein (FITC) Alexa Fluor® 488, Cy2®


XF1072 XF2037 XF3080

475AF20 500DRLP 510AF23


XF1073 XF2010 XF3084

475AF40 505DRLP 535AF45

This set consists of wide bandpass filters for collecting maximal Exciter excitation and emission energy. It is not as discriminating of Dichroic yellow and red emitting fluorophores as the XF100-2 or XF116-2. Emitter

XF1087 XF2077 XF3105

470AF50 500DRLP 545AF75

Narrow band filters can help to reduce sample auto-fluorescence. Also useful for discrimination from red emitting fluorophores such as mRFP.

eGFP, Fluorescein (FITC) XF100-2 Alexa Fluor® 488 Cy2®, DiO, Fluo-4 eGFP, Fluorescein (FITC) XF100-3 Alexa Fluor® 488 Cy2®, DiO, Fluo-4

YFP XF104-2 Rhodamine Green™ Alexa Fluor® 532


XF1068 XF2030 XF3074

500AF25 525DRLP 545AF35

TRITC XF101-2 Long pass emission filter. Cy3®, Alexa Fluor® 555 MitoTracker® Orange


XF1074 XF2017 XF3085


TRITC, Alexa Fluor® 555 XF108-2 Cy3®, DsRed2 MitoTracker® Orange


XF1074 XF2017 XF3083

525AF45 560DRLP 595AF60

Texas Red®/Texas Red®-X XF102-2 Cy3.5® MitoTracker® Red


XF1067 XF2029 XF3081

560AF55 595DRLP 645AF75

Cy5®, Alexa Fluor® 647 XF110-2 APC (allophycocyanin) DiD (DilC18(5))


XF1069 XF2035 XF3076

630AF50 650DRLP 695AF55

It is very difficult to see emissions at these wavelengths with the unaided eye. B/W camera is typically used to capture signal.


13

Standard Filters

Products

are available in the following sizes. Excitation and emission filters: 18, 20, 22, and 25 mm round. Dichroic beamsplitters: 18 x 26, 20 x 28, 21 x 29, and 25.7 x 36 mm rectangular. Dichroics also available as 18, 20, 22, and 25 mm round.

Ships within 5-10 business Days!

Standard Filters Fluorophores

Filter sets are arranged by fluorophores and emission wavelength. Filter Set #

Applications

Components Type

Part #

Description

Deep UV Combinations XF01 Deep UV excitation/near UV emission deep purple color


XF1000 XF2000 XF3000

254WB25 290DCLP 330WB60

DAPI XF02-2 Wide band excitation filter with longpass emission Hoechst 33342 & 33258 filter AMCA/AMCA-X


XF1001 XF2001 XF3097

330WB80 400DCLP 400ALP

DAPI XF05-2 Good with mercury arc lamp Hoechst 33342 & 33258 AMCA/AMCA-X


XF1005 XF2001 XF3097


BFP XF129-2 See XF131 for bandpass emission filter LysoSensor™ Blue (pH 5)


XF1075 XF2004 XF3088


CCF2 (GeneBLAzer™) XF106-2 Combines blue and green emission colors


XF1076 XF2040 XF3088


DAPI XF03 Wide band excitation filter with bandpass emission Hoechst 33342 & 33258 filter AMCA/AMCA-X


XF1001 XF2001 XF3002

330WB80 400DCLP 450AF65

Cascade Blue® XF10-2 LysoSensor™ Blue


XF1094 XF2001 XF3002

380AF15 400DCLP 450AF65

BFP, LysoSensor™ Blue (pH5) XF131


XF1075 XF2004 XF3002

387AF28 410DRLP 450AF65

SpectrumBlue® XF11 Good for Hg lamp Cascade Blue® CPM, Pacific Blue™


XF1006 XF2059 XF3002

400DF15 415DRLP 450AF65

Cascade Yellow™ XF13-2 SpectrumAqua® SYTOX® Blue


XF1008 XF2040 XF3091

405DF40 435DRLP 460ALP

Pacific Blue™ XF119-2


XF1076 XF2040 XF3078

400AF30 435DRLP 465AF30

wtGFP XF170 Longpass emission filter uses primary excitation peak


XF1076 XF2040 XF3087


CFP XF130-2 See XF114-2 for bandpass emission filter SpectrumAqua®


XF1071 XF2034 XF3087


Fura Red™, Lucifer Yellow XF77-2 LysoSensor™ Green (pH5)


XF1031 XF2027 XF3092


AmCyan XF168


XF1101 XF2091 XF3119

440AF40 465DRLP 505AF50

wtGFP XF169 Bandpass emission filter uses primary excitation peak


XF1076 XF2040 XF3080

400AF30 435DRLP 510AF23

14



Pricing


We provide the most competitive prices. For current product pricing, please visit www.omegafilters.com or contact us at:


s [email protected] + 1.802.254.2690 (outside USA) 1 .866.488.1064 (toll free within USA only)



Applications

Components Type

Part #

Description

Fura Red™ (high calcium) XF18-2 Broad excitation filter DiA (4-Di-16-ASP)


XF1012 XF2008 XF3093


Acridine orange (+RNA) XF19-2 Auramine O-Feulgen


XF1013 XF2008 XF3093


Fluoro-Gold™ (high pH) XF08 Brighter emission and background than XF09 LysoSensor™ Yellow/Blue (pH 3)


XF1005 365WB50 XF2001 400DCLP XF3006 OG515

GFP (sapphire) XF76 Cascade Yellow™


XF1008 XF2006 XF3003

405DF40 450DCLP 520DF40

Alexa Fluor® 430 XF14-2 Green emission compared to XF15 Cascade Yellow™ Lucifer Yellow


XF1009 XF2007 XF3105

425DF45 475DCLP 545AF75

Alexa Fluor® 430 XF15 Yellow emission compared XF14 LysoSensor™ Green (pH 5) Quinacrine Mustard


XF1009 425DF45 XF2007 475DCLP XF3006 OG515

CoralHue Azami Green XF171


XF1102 XF2027 XF3003

475AF30 485DRLP 520DF40

Fluorescein (FITC) XF22 Higher signal-to-noise than XF25 Alexa Fluor® 488 Cy2®, BODIPY® FL


XF1099 XF2010 XF3017

485DF20 505DRLP 530DF30

YFP XF105-2 See XF104-2 for bandpass emission filter Rhodamine Green™ Alexa Fluor® 532


XF1068 XF2030 XF3082


Fluorescein (FITC) XF25 Not suitable for multicolor imaging Cy2®, Alexa Fluor® 488 BODIPY® FL


XF1099 485DF20 XF2010 505DRLP XF3018 OG530

Fluoro-Gold™ (high pH) XF09 Excellent for multiwavelength work in red Aniline Blue


XF1005 XF2001 XF3007

365WB50 400DCLP 535DF35

LysoSensor™ Green (pH 5) XF71 Auramine O-Feulgen


XF1031 XF2026 XF3007

450DF55 495DRLP 535DF35

Emerald XF172


XF1072 XF2077 XF3084

475AF20 500DRLP 535AF45

Fluorescein (FITC) XF23 Better photopic color rendition Alexa Fluor® 488 Cy2®, BODIPY® FL


XF1099 XF2010 XF3007

485DF20 505DRLP 535DF35

Spectrum Green® XF144


XF1011 XF2008 XF3007

490DF20 515DRLP 535DF35

SpectrumGreen® #2 XF30 Brighter emission and background than XF99 Alexa Fluor® 532 BODIPY® 530/550


XF1080 510DF25 XF2012 540DRLP XF3021 OG550


15

Standard Filters

Products





Applications

Components Type

Part #

Description

Auramine XF20 Narrow band emission filter


XF1013 XF2008 XF3014

470DF35 515DRLP 560DF15

Alexa Fluor® 532 XF99 Yellow emission filter


XF1080 XF2012 XF3014

510DF25 540DRLP 560DF15

DsRed2 XF111-2


XF1077 XF2015 XF3089


Spectrum Gold® XF145


XF1019 XF2017 XF3022

535DF35 560DRLP 580DF30

TRITC, SpectrumOrange® XF37 Optimized for Hg lamp Cy3®, Alexa Fluor® 555 MitoTracker® Orange


XF1020 XF2062 XF3022

546DF10 555DRLP 580DF30

TRITC, SpectrumOrange® XF32 Yellow emission filter Cy3®, MitoTracker® Orange Alexa Fluor® 555


XF1019 XF2015 XF3024

535DF35 570DRLP 590DF35

TRITC, Cy3®, SpectrumOrange® XF34 Red emission filter MitoTracker® Orange Alexa Fluor® 555


XF1019 535DF35 XF2015 570DRLP XF3016 OG590

TRITC, Cy3®, SpectrumOrange® XF38 Optimized for Hg Lamp Alexa Fluor® 555 MitoTracker® Orange


XF1020 546DF10 XF2015 570DRLP XF3016 OG590

tdTomato XF173


XF1103 XF2015 XF3083

535AF30 570DRLP 595AF60

Ethidium homodimer-1 (EthD-1) XF28


XF1080 XF2009 XF3019

510DF25 550DCLP 605DF50

TRITC, SpectrumOrange® XF33 Photomicrography Cy3®, MitoTracker® Orange Alexa Fluor® 555


XF1019 XF2015 XF3019

535DF35 570DRLP 605DF50

Cy3.5® XF41 Orange emission filter MitoTracker® Red


XF1022 XF2019 XF3027

560DF40 590DRLP 610DF20

AsRed 2, mStrawberry XF174


XF1104 XF2018 XF3120

555AF30 580DRLP 610AF50

XRITC Cy3.5®, XF40-2 MitoTracker® Red SNARF®-1 (high pH), Alexa Fluor® 568/594


XF1022 XF2019 XF3094


Keima Red XF176 NEW! Long pass emission filter to give a rich red color in single label schemes


XF1071 XF2019 XF3094


Keima Red XF177 NEW! Same excitation and emission filter as XF176, but blue shifted dichroic mirror, multicolor applications


XF1071 XF2007 XF3094

440AF21 475DCLP 610ALP

16



Pricing


We provide the most competitive prices. For current product pricing, please visit www.glenspectra.co.uk or contact us at:  





Applications

Components Type

Part #

Description

Keima Red XF178 NEW! Bandpass version of XF177, multi-label applications


XF1071 XF2007 XF3025

440AF21 475DCLP 615DF45

Rhodamine B, Rhodamine Red™ XF39 X-Rhodamine (XRITC) Nile Red, CryptoLight CF-5


XF1021 XF2018 XF3025

550DF30 580DRLP 615DF45

Spectrum Orange® XF146


XF1017 XF2018 XF3025

555DF27 580DRLP 615DF45

Texas Red®/Texas Red®-X XF43 Alexa Fluor® 594


XF1044 XF2020 XF3028

575DF25 600DRLP 630DF30

Acridine orange (+RNA) XF21 Di-4 ANEPPS


XF1014 XF2009 XF3015

480DF60 550DCLP 635DF55

Propidium Iodide XF35 Ethidium bromide Nile Red


XF1019 XF2016 XF3015

535DF35 560DCLP 635DF55

Texas Red®/Texas Red®-X XF42 SpectrumRed® Alexa Fluor® 594, CryptoLight CF-6


XF1022 XF2019 XF3015

560DF40 590DRLP 635DF55

mCherry XF175


XF1067 XF2020 XF3081

560AF55 600DRLP 645AF75

Propidium Iodide XF103-2 Ethidium bromide Nile Red


XF1074 XF2016 XF3081

525AF45 560DCLP 645AF75

Fura Red™ XF27


XF1011 XF2009 XF3012

490DF20 550DCLP 660DF50

7-Aminoactinomycin D (7-AAD) XF36 SensiLight P-1


XF1019 XF2016 XF3029

535DF35 560DCLP 660DF32

APC (allophycocyanin) XF44 BODIPY® 630/650-X CryptoLight CF-2


XF1024 XF2021 XF3029

590DF45 630DRLP 660DF32

APC (allophycocyanin) XF45 DIPY® 630/650-X CryptoLight CF-2, SensiLight P-3


XF1025 XF2021 XF3030

610DF20BO630DRLP 670DF40

Cy5® XF46 Excitation filter optimal for 633 HeNe laser line BODIPY® 630/650-X Alexa Fluor® 633/647


XF1026 XF2022 XF3030

633NB3.0 640DRLP 670DF40

Cy5® XF47 Non-visual narrow emission BODIPY® 630/650-X Alexa Fluor® 660


XF1027 XF2035 XF3031

640DF20 650DRLP 682DF22

Alexa Fluor® 660/680, Cy5.5® XF138-2 Red Diode & HeNe lasers


XF1085 XF2075 XF3104

680ASP 690DRLP 690ALP


17

Standard Filters

Products



Standard Filters


Fluorophores

Filter Set #

Applications

Components Type

Part #

Description

Cy5® XF70 Non-visual Broad excitation BODIPY® 630/650-X Alexa Fluor® 660


XF1030 XF2023 XF3034

610DF70 660DRLP 690DF40

SpectrumFRed® XF147


XF1027 XF2035 XF3034

640DF20 650DRLP 690DF40

Alexa Fluor® 633/647, Cy5® XF140-2 Hg Arc lamp


XF1082 XF2072 XF3076

607AF75 650DRLP 695AF55

Alexa Fluor® 680, Cy5.5® XF48-2 Non-visual detection An IR sensitive detector must be used


XF1028 XF2024 XF3095


Alexa Fluor® 660/680, Cy5.5® XF141-2 Non-visual detection An IR sensitive detector must be used


XF1095 XF2082 XF3113

655AF50 692DRLP 710AF40

Alexa Fluor® 700 XF142-2 Non-visual detection An IR sensitive detector must be used


XF1096 XF2083 XF3114

685AF30 708DRLP 730AF30

Alexa Fluor® 750, Cy7® XF143-2 Non-visual detection An IR sensitive detector must be used


XF1097 XF2084 XF3115


XF22 Standard Filter Set for FITC Representation of Typical Standard Filter Performance 100 –

XF1015 90 – XF2010 XF3017 80 –

Transmission (%)

70 – 60 – 50 – 40 – 30 – 20 – 10 – 0– 300

350

400

450

500

550

600

650

Wavelength (nm)

18


Note

Pricing

Filter Sets with a dash indicates revision level.

We provide the most competitive prices. For current product pricing, please visit www.glenspectra.co.uk or contact us at:


 

Filter Sets Reference Table Filter Set #

Product Category Page #

Filter Set #


Filter Set #


Filter Set #


QMAX-Blue

QuantaMAX

12

XF144

Vivid Standard

15

XF210

M-FISH

36

XF44

Vivid Standard

17

QMAX-FITC/TRITC QuantaMAX

12

XF145

Vivid Standard

16

XF22

Vivid Standard

15

XF45

Vivid Standard

17

QMAX-FRed

QuantaMAX

12

XF146

Vivid Standard

17

XF23

Vivid Standard

15

XF46

Vivid Standard

17

QMAX-Green

QuantaMAX

12

XF147

Vivid Standard

18

XF231

M-FISH

36

XF47

Vivid Standard

17

QMAX-Red

QuantaMAX

12

XF15

Vivid Classic

15

XF232

M-FISH

36

XF48-2

Vivid Plus

18

QMAX-Yellow

QuantaMAX

12

XF151-2

FRET

29

XF25

Vivid Classic

15

XF50

Multi-band - Dual

XF01

Vivid Standard

14

XF152-2

FRET

29

XF26

Ratio Imaging

37

XF50-1

Pinkel

XF02-2

Vivid Plus

14, 33

XF153

Photoswitchable Protein

35

XF27

Vivid Standard

17

XF51

Multi-band - Dual

38

XF03

Vivid Standard

14

XF154

Multi-band - Triple

28, 38

XF28

Vivid Standard

16

XF52

Multi-band - Dual

28, 38

XF04-2

Ratio Imaging

37

XF154-1

Pinkel

34

XF29

Ratio Imaging

37

XF52-1

Pinkel

XF05-2

Quantum Dots

14, 33

XF155

Sedat

35

XF30

Vivid Classic

15

XF53

Multi-band - Dual

38

XF06

Vivid Standard

13, 36

XF156

Sedat

35

XF300

Quantum Dots

32

XF53-1

Pinkel

34

XF07

Ratio Imaging

37

XF157

Sedat

35

XF301-1

Quantum Dots

32

XF56

Multi-band - Triple

38

XF08

Vivid Classic

15

XF158

FRET

29

XF301-2

Quantum Dots

32

XF57

Multi-band - Quad Set

38

XF09

Vivid Standard

15

XF159

FRET

29

XF302-1

Quantum Dots

32

XF57-1

Pinkel

35

XF100-2 ALPHA Vivid

13, 27

XF16

Ratio Imaging

37

XF302-2

Quantum Dots

32

XF59

Multi-band - Dual

38

XF100-3 ALPHA Vivid

13, 27

XF160

FRET

29

XF303-1

Quantum Dots

32

XF59-1

Pinkel

34

XF101-2 ALPHA Vivid

13, 28

XF162

FRET

29

XF303-2

Quantum Dots

32

XF63

Multi-band - Triple

38

14

XF163

FRET

29

XF304-1

Quantum Dots

32

XF63-1

Pinkel

34

13, 28

XF164

FRET

29

XF304-2

Quantum Dots

32

XF66

Multi-band - Triple

38

XF103-2 ALPHA Vivid

17

XF165

FRET

29

XF305-1

Quantum Dots

32

XF67

Multi-band - Triple

38

XF104-2 ALPHA Vivid

13, 27

XF166

FRET

29

XF305-2

Quantum Dots

32

XF67-1

Pinkel

34

XF105-2 ALPHA Vivid

15, 27

XF167

FRET

29

XF306-1

Quantum Dots

32

XF68

Multi-band - Triple

38

XF10-2

Vivid Standard

XF102-2 ALPHA Vivid

28, 38 34

34

XF106-2 GeneBLAzer

14

XF168 ALPHA Vivid

14, 27

XF306-2

Quantum Dots

32

XF68-1

Pinkel

34

XF108-2 ALPHA Vivid

13, 28

XF169 ALPHA Vivid

14, 27

XF307-1

Quantum Dots

32

XF69

Multi-band - Triple

38

XF11

37

XF307-2

Quantum Dots

32

XF69-1

Pinkel

34

XF110-2 ALPHA Vivid

13, 28

XF170 ALPHA Vivid

14, 27

XF308-1

Quantum Dots

32

XF70

Vivid Standard

18

XF111-2 ALPHA Vivid

16, 28

XF171

15, 27

XF308-2

Quantum Dots

32

XF71

Vivid Standard

15

XF114-2 ALPHA Vivid

13, 27

XF172 ALPHA Vivid

15, 27

XF309-1

Quantum Dots

32

XF72

Ratio Imaging

37

XF115-2 ALPHA Vivid

13, 27

XF173 ALPHA Vivid

16, 28

XF309-2

Quantum Dots

32

XF73-2

Caged Compound

40

XF116-2 ALPHA Vivid

13, 27

XF174 ALPHA Vivid

16, 28

XF31

Ratio Imaging

37

XF74-2

Caged Compound

40

XF119-2 ALPHA Vivid

14

XF175 ALPHA Vivid

17, 28

XF32

Vivid Standard

16

XF75

Caged Compound

XF124 GeneBLAzer

37

XF176 ALPHA Vivid

16, 28

XF320

Quantum Dots

33

XF76

Vivid Standard

XF129-2 ALPHA Vivid

14, 27

XF177 ALPHA Vivid

16, 28

XF33

Vivid Standard

16

XF77-2

Vivid Plus

14

XF130-2 ALPHA Vivid

14, 27

XF178

Vivid Plus

17, 28

XF34

Vivid Classic

16

XF79-2

Ratio Imaging

37

XF131

Vivid Plus

14, 27

XF18-2

Vivid Plus

15

XF35

Vivid Standard

17

XF88-2

FRET

29

XF13-2

Vivid Plus

14

XF19-2

Vivid Plus

15

XF36

Vivid Standard

17

XF89-2

FRET

29

XF133

Ratio Imaging

37

XF20

Vivid Standard

16

XF37

Vivid Standard

16

XF92

Multi-band - Dual

38

XF135

Multi-band - Dual

28, 38

XF201

M-FISH

36

XF38

Vivid Classic

16

XF93

Multi-band - Triple

38

XF135-1

Pinkel

Vivid Standard

14

XF17

Ratio Imaging Vivid Plus

40 15, 27

34

XF202

M-FISH

36

XF39

Vivid Standard

17

XF93-1

Pinkel

34

XF138-2 ALPHA Vivid

17

XF203

M-FISH

36

XF401

Quanta-Vivid

13

XF94

Multi-band - Triple

38

XF140-2 ALPHA Vivid

18

XF204

M-FISH

36

XF402

Quanta-Vivid

13

XF95

Multi-band - Triple

38

XF141-2 ALPHA Vivid

18

XF206

M-FISH

36

XF40-2

Vivid Plus

16

XF99

Vivid Standard

16

XF14-2

15

XF207

M-FISH

36

XF41

Vivid Standard

16

XF142-2 ALPHA Vivid

18

XF208

M-FISH

36

XF42

Vivid Standard

17

XF143-2 ALPHA Vivid

18, 36

XF21

Vivid Standard

17

XF43

Vivid Standard

17

Vivid Plus


19

Stock Filters

Products

Standard Filters

Excitation Filters by CWL

(center wavelength) Descriptor

XF1000

254WB25 330WB80

XF1093

340AF15

QMAX/EX355-405

355-405

XF1004

355HT15

XF1005

365WB50

XF1094

380AF15

XF1401

380DF30

XF1057


(center wavelength)

Stock Standard

XF1001



Excitation Filters by CWL Part #



l

Descriptor

Stock Standard

XF1050

490-550DBEX

l

Descriptor

Stock Standard

XF2000

290DCLP

l l

490-577DBEX

XF2050

385-485-560TBDR

XF1049

490-605DBEX

l

XF2041

385-502DBDR

XF1011

490DF20

l

XF2056

390-510-630TBDR

XF1068

500AF25

XF2003

390DRLP

l

XF1088

500AF30

XF2047

395-540DBDR

l

l

QMAX/EX510-560

510-560

XF2048

400-477-575TBDR

l

l

XF1080

510DF25

l

XF2046

400-485-558-640QBDR

l

385-485-560TBEX

l

XF1018

515NB2.0

l

XF2045

400-485-580TBDR

l

XF1059

386-485-560TBEX

l

XF1203

520AF18

l

XF2061

400-485DBDR

l

XF1075

387AF28

XF1074

525AF45

l

XF2051

400-495-575TBDR

l

XF1052

390-486-577TBEX

l

XF1403

525QV45

l

XF2055

400-535-635TBDR

l

XF1058

390-486-577TBEX

l

QMAX/EX530-570

530-570

l

XF2001

400DCLP

l

XF1054

390-520DBEX

l

XF1103

535AF30

QMAX/DI410LP

410LP

l

XF1055

400-477-580TBEX

l

XF1019

535DF35

XF2004

410DRLP

l

400-495-575TBEX

l

XF1077

540AF30

l

XF2002

415DCLP

l

XF1048

400-500DBEX

l

l l

l

l

l

Part #

XF1051

XF1098

l

Part #

by Cut-On

l l

l

l

l

l l

l

XF1204

546AF18

l

XF2059

415DRLP

l

XF1020

546DF10

l

XF2040

435DRLP

l

XF1062

550-640DBEX

l

XF2065

436-510DBDR

l

XF1021

550DF30

l

XF2090

445-510-600TBDR

l l

XF1065

400-510-630TBEX

l

XF1076

400AF30

l

XF1006

400DF15

XF1053

405-490-555-650QBEX

l

XF1104

555AF30

l

XF2006

450DCLP

XF1008

405DF40

l

XF1043

555DF10

l

XF2034

455DRLP

l

XF1064

415-535-630TBEX

l

XF1017

555DF27

l

XF2091

465DRLP

l

XF1301

415WB100

l

XF1067

560AF55

XF2007

475DCLP

l

XF1009

425DF45

XF1045

560DF15

l

XF2054

485-555-650TBDR

l

XF1100

436-495-582TBEX

l

XF1022

560DF40

l

XF2039

485-555DBDR

l

XF1078

436-510DBEX

l

XF1206

572AF15

l

QMAX/DBDR485-560 485-560DBDR

XF1201

436AF8

l

XF1044

575DF25

l

XF2027

485DRLP

l

XF1079

436DF10

l

XF1207

580AF20

l

XF2043

490-550DBDR

l

XF1071

440AF21

l

XF1024

590DF45

l

XF2044

490-575DBDR

XF1402

440QV21

l

XF1082

607AF75

l

XF2042

490-600DBDR

l

XF1025

610DF20

l

XF2026

495DRLP

l

l

XF1101

l

l

440AF40

l

l

l

QMAX/EX450-490

450-490

XF1030

610DF70

QMAX/DI500LP

500LP

XF1031

450DF55

l

QMAX/EX620-650

620-650

l

XF2037

500DRLP

XF1012

455DF70

l

XF1069

630AF50

l

XF2077

500DRLP

l

XF1087

470AF50

XF1026

633NB3.0

l

XF2010

505DRLP

l

XF1013

470DF35

l

XF1208

640AF20

l

XF2031

505DRLPXR

l

XF1072

475AF20

l

XF1027

640DF20

l

XF2008

515DRLP

l

XF1102

475AF30

l

XF1095

655AF30

l

XF2058

515DRLPXR

l

XF1073

475AF40

XF1046

655DF50

l

XF2030

525DRLP

XF1014

480DF60

XF1210

665AF32

l

XF2013

540DCLP

l

XF1028

670DF20

l

XF2012

540DRLP

l

QMAX/DBEX485-560 485-560DBEX

l

l

l

l l

l

l l

l

XF1063

485-555-650TBEX

l

XF1085

680ASP

l

XF2203

545DRLP

l

XF1202

485AF20

l

XF1096

685AF30

l

XF2009

550DCLP

l

XF1042

485DF15

l

XF1097

730AF45

l

XF2053

555-640DBDR

l

XF1015

485DF22

20

l


Decoding filter descriptions

Pricing

Most filter descriptions are identified by center wavelength, filter design code and full width half maximum. For example, a 490DF20 filter has a 490-nm center wavelength, and a 20-nm bandwidth, or a 480- to 500-nm passband (all values are approximate). A 490DF20 passband is 490 ±10 nm, not 490 ±20 nm.

We provide the most competitive prices. For current product pricing, please visit www.glenspectra.co.uk or contact us at: 


[email protected]




Emission Filters by CWL

by Cut-On

Part #

Descriptor

XF2062

555DRLP

Stock Standard

QMAX/DI560LP

560LP

XF2016

560DCLP

XF2017

560DRLP

XF2032

565DRLPXR

XF2015

570DRLP

QMAX/DI580LP

580LP

XF2018

580DRLP

XF2011

Emission Filters by CWL

(center wavelength)

l

l

Part #

Descriptor

XF3000

330WB60

(center wavelength)

Stock Standard

XF3097

400ALP

XF3004

405DF43

QMAX/EM420-480

420-480

l

XF3088

435ALP

l

XF3061

445-525-650TBEM

XF3002

450AF65

l

XF3058

457-528-633TBEM

585DRLP

l

XF3063

XF2019

590DRLP

l

XF2029

595DRLP

XF2020

600DRLP

XF2014

610DRLP

XF2021 XF2022

l

l

Descriptor

Stock Standard

XF3074

545AF35

l

XF3105

545AF75

l

XF3021 OG550

l

XF3014

560DF15

l

l

XF3085

565ALP

l

l

XF3302

565WB20

l

QMAX/EM570-600

570-600

l

l

XF3089

575ALP

460-520-602TBEM

l

XF3022

580DF30

XF3059

460-520-603-710QBEM

l

XF3090

585ALP

XF3054

460-550DBEM

XF3303

585WB20

l

l

XF3069

460-560-690TBEM

l

XF3024

590DF35

l

l

XF3091

460ALP

l

XF3016 OG590

630DRLP

l

XF3118

465-535-640TBEM

l

XF3066

595-700DBEM

l

640DRLP

l

XF3078

465AF30

l

XF3083

595AF60

l

470-530-620TBEM

l

QMAX/EM600-650

600-650

l

470-590DBEM

l

XF3019

605DF50

l

XF3099

475-550DBEM

l

XF3304

605WB20

l

XF3075

480AF30

l

XF3120

610AF50

XF3401

480QV30

l

XF3094

610ALP

l

l

l

l

l

XF3116

l

Part #

l

l

l

l

l l

l

XF2035

650DRLP

XF2072

650DRLP

QMAX/DI660LP

660LP

XF2023

660DRLP

XF2033

675DCSPXR

XF2024

690DRLP

l

XF3087

480ALP

l

XF3027

610DF20

l

XF2075

690DRLP

l

XF3068

495-575-700TBEM

l

XF3110

615AF30

l

XF2082

692DRLP

l

XF3005

495DF20

l

XF3025

615DF45

XF2083

708DRLP

l

XF3092

500ALP

l

XF3020

620DF35

l

XF2084

760DRLP

l

XF3108

502ALP

l

XF3009

625DF20

l

XF3119

505AF50

l

XF3028

630DF30

l

QMAX/EM510-560

510-560

XF3015

635DF55

l

XF3080

510AF23

XF3023

640DF35

l

XF3086

510ALP

l

XF3081

645AF75

l

XF3043

510WB40

l

XF3402

645QV75

l

XF3067

515-600-730TBEM

l

XF3305

655WB20

l

XF3093

515ALP

l

XF3029

660DF32

l

XF3006 OG515

l

XF3012

660DF50

l

XF3056

l

l

l l

l

XF3060

l

520-580DBEM

QMAX/DBEM520-610 520-610DBEM

l

l

l

l

l

QMAX/EM670-750

670-750

l

XF3030

670DF40

l

XF3003

520DF40

l

XF3031

682DF22

l

XF3055

522-637DBEM

l

XF3104

690ALP

l

XF3301

525WB20

l

XF3034

690DF40

l

XF3057

528-633DBEM

l

XF3076

695AF55

XF3082

530ALP

l

XF3095

700ALP

l

XF3017

530DF30

l

XF3113

710AF40

l

XF3018 OG530

l

XF3100

710ASP

l

XF3114

730AF30

l

XF3079

535AF26

l

XF3084

535AF45

l

XF3115

770ALP

XF3011

535DF25

l

XF3307

800WB80

l

XF3007

535DF35

l

XF3308

840WB80

l


l l

21

Products

3rd Millennium Filters A ssembly Diameter – 25mm A ssembly Height – 5.5mm C lear Aperture – 21.3mm S ubstrates – SiO2 substrates with

Design Features H ermetic Assembly F used Silica Substrates A ccurate Band Placement S teeper Edge Slopes

broadband anti-reflection coating

3rd Millenium Filters Filter Type

Wavelength Range

Transmission

Attenuation Range

Attenuation OD

Longpass

Cut-On from 400-700nm >/= 90% peak UV to cut-on OD 12 in theory at every 10nm OD 6 min

Cut-On from 700-800nm at every 20nm

Size

25 mm

Cut-On from 800-1100nm at every 50nm Shortpass

Cut-Off from 400-700nm >/= 90% peak Cut-off to 1.3x cut-off * OD 12 in theory at every 10nm OD 6 min

Cut-Off from 700-800nm at every 20nm

25 mm

Cut-Off from 800-1100nm at every 50nm Bandpass

Cut-On + Cut-Off from

>/= 80% peak

400-700nm at every 10nm

OD 6 min

700-800nm at every 20nm

UV to 1.3x cut-off *

OD 12 in theory 25 mm

800-1100nm at every 50nm * For extended long wavelength attenuation see Attenuation Extension

All 3rd Millennium filters are offered every 10nm from 400-700nm, every 20nm from 700-800nm and every 50nm from 800nm to 1100nm.

Attenuation Extension: When using a silicon detector, in some cases a blocker filter is required to extend the attenuation range to 1100nm nominally. For optimal performance we recommend locating the blocker filter in a position remote from the 3rd Millennium filter. Standard blocker filter is 25mm. Other sizes are available.

22


Pricing We provide the most competitive prices. For current product pricing, please visit www.glenspectra.cO.uk or contact us at:  



Custom Filters – OEM Production Quantities 3rd Millennium filters are specified by the critical cut-on and cut-off edges, resulting in much more accurate band placement and bandwidth. To specify a filter: Longpass – specify a cut-on wavelength Example: Part # 3RD650LP Shortpass – specify a cut-off wavelength Example: Part # 3RD 520SP Bandpass – specify a cut-on & cut-off wavelength Example: Part # 3RD 580LP-600SP

Automated Manufacturing 3rd Millenium filters are produced in custom engineered coating equipment that progresses from raw material to complete assemblies in a shortened manufacturing cycle. The coating chamber is load-locked so that it remains under stable high vacuum conditions between coating cycles. C ontrol and design of manufacturing processes lead to yield and product uniformity. S hort manufacturing cycles results in controlled inventories and shorter lead times. 

Design 3rd Millennium filters are manufactured using Omega Optical’s proprietary ALPHA Technology, a process which produces exceptionally steep cut-on and cut-off slopes. This results in more precise location of cut-on and cut-off points, the ability to place transmission and rejection regions extremely close together, and higher attenuation between the passband and the rejection band. Bandpass filters are made using a combination of an ALPHA longpass (cut-on) and ALPHA shortpass (cut-off). Longpass and shortpass filters are each made using a single surface coating.

Higher Throughput Passbands can be made wider and still achieve the blocking requirements of narrower, less steep designs. Wider ALPHA bandpass filters outperform narrower standard designs by providing improved transmission, better blocking, and higher signal-to-noise. Optical Density Curve

Transmission Curve 16 14

3RD Millennium Bandpass

80 60

Optical Density

Transmission (%T)

100

Standard Fabry Perot

40

Bandpass

3RD Millennium Bandpass

12 10 8 6 4

20

Standard Fabry Perot Bandpass

2 0 500

520

540

560

Wavelength (nm)

580

600

0 425

475

525

575

625

675

Wavelength (nm)


23

Stock Filters



Products

Standard Filters



Fluorophores

Fluorophores

(A-B)

Fluorophore

EX

EM

Best Set

(B-C)

Stock Standard

Fluorophore

EX

EM

Best Set

Stock Standard

BODIPY® 505/515

502

510

QMAX-Green

l

BODIPY® 530/550

533

550

XF108-2

l

AcGFP1

475

505

XF100-2

Acridine Yellow

470

550

XF23

Acridine orange (+DNA)

500

526

XF104-2

l

BODIPY® 558/568

558

568

XF108-2

l

Acridine orange (+RNA)

460

650

QMAX-Blue

l

BODIPY® 564/570

564

570

XF108-2

l

Alexa Fluor® 350

347

442

QMAX-Blue

l

XF BODIPY® 581/591

582

590

XF102-2

l

Alexa Fluor® 405

401

421

XF11

l

BODIPY® 630/650-X

630

650

XF45

Alexa Fluor® 430

434

540

XF14-2

l

BODIPY® 650/665-X

650

665

XF110-2

l

Alexa Fluor® 488

495

519

XF100-2

l

BODIPY® 665/676

665

676

XF110-2

l

Alexa Fluor® 500

503

525

XF104-2

l

BTC

401/464 529

XF133

Alexa Fluor® 532

531

554

XF104-2

l

Calcein

494

517

XF100-2

l

Alexa Fluor® 546

556

573

XF108-2

l

Calcein Blue

375

420

XF06

l

Alexa Fluor® 555

553

568

XF108-2

l

Calcium Crimson™

590

615

XF102-2

l

Alexa Fluor® 568

579

604

XF102-2

l

Calcium Green-1™

506

531

XF104-2

l

Alexa Fluor® 594

591

618

XF102-2

l

Calcium Orange™

549

576

XF108-2

l

Alexa Fluor® 610

612

628

XF102-2

l

Calcofluor® White

350

440

XF06

l

Alexa Fluor® 633

632

647

XF140-2

5-Carboxyfluorescein (5-FAM)

492

518

XF100-2

l

Alexa Fluor® 647

653

669

XF110-2

5-Carboxynaphthofluorescein

598

668

XF102-2

l

Alexa Fluor® 660

663

690

XF141-2

l

6-Carboxyrhodamine 6G

525

555

XF104-2

l

Alexa Fluor® 680

679

702

XF141-2

l

5-Carboxytetramethylrhodamine (5-TAMRA) 542

568

XF108-2

l

Alexa Fluor® 700

702

723

XF142-2

l

Carboxy-X-rhodamine (5-ROX)

574

602

XF102-2

l

Alexa Fluor® 750

749

775

XF143-2

l

Cascade Blue®

400

420

XF06

l

Alexa Fluor® 488/546 FRET

495

573

XF164

FRET

Cascade Yellow™

402

545

XF106

l

Alexa Fluor® 488/555 FRET

495

568

XF164

FRET

CCF2 (GeneBLAzer™)

402

520

XF106

l

Alexa Fluor® 488/Cy3® FRET

495

570

XF165

FRET

Cell Tracker Blue

353

466

XF06

l

Allophycocyanin (APC)

650

660

XF110-2

l

Cerulean

433

475

XF114-2

l

AMCA/AMCA-X

345

445

XF06

l

CFP (Cyan Fluorescent Protein)

434

477

XF114-2

l

AmCyan1

458

489

XF168

l

CFP/DsRed2 FRET

434

583

XF152

FRET

7-Aminoactinomycin D (7-AAD)

546

647

XF103-2

l

CFP/YFP FRET

434

527

XF88

FRET

7-Amino-4-methylcoumarin

351

430

XF06

Chromomycin A3

450

470

XF114-2

l

Aniline Blue

370

509

XF09

l

Cl-NERF (low pH)

504

540

XF104-2

l

ANS

372

455

XF05-2

l

CoralHue Azami Green

492

505

XF171

l

AsRed2

578

592

QMAX-Yellow

CoralHue Dronpa Green

503

518

CALL

l

ATTO-TAG™ CBQCA

465

560

XF18-2

l

CoralHue Kaede Green

508

518

XF153

l

ATTO-TAG™ FQ

486

591

XF115-2

l

CoralHue Kaede Red

572

580

XF153

l

Auramine O-Feulgen

460

550

XF20

l

CoralHue Keima Red

440

620

CALL

l

Azami Green

493

505

QMAX-Green

CoralHue Kusabira Orange (mKO)

552

559

XF108-2

l

BCECF

503

528

XF16

CoralHue Midoriishi-Cyan (MiCy)

472

492

XF116-2

l

BFP (Blue Fluorescent Protein)

382

448

QMAX-Blue

CPM

385

471

XF11

BFP/DsRed2 FRET

382

583

XF159

FRET

6-CR 6G

518

543

XF104-2

BFP/eGFP FRET

382

508

XF89-2

FRET

CryptoLight CF-2

584/642 657

XF44

l

BFP/YFP FRET

382

527

XF158

FRET

CryptoLight CF-5

566

597

XF39

l

BOBO™-1, BO-PRO™-1

462

481

XF114-2

l

CryptoLight CF-6

566

615

XF42

l

BOBO™-3, BO-PRO™-3

570

604

XF102-2

l

CTC Formazan

450

630

XF21

l

BODIPY® FL

505

513

XF100-2

l

Cy2®

489

506

XF100-2

l

BODIPY® TMR

542

574

XF108-2

l

Cy3®

550

570

XF108-2

l

BODIPY® TR-X

589

617

XF102-2

l

Cy3.5®

581

596

XF102-2

l

BODIPY® 492/515

490

515

XF100-2

l

Cy5®

649

670

QMAX-Far Red

l

BODIPY® 493/ 503

500

506

QMAX-Green

l

Cy5.5®

675

694

XF141-2

l

BODIPY® 500/ 510

509

515

XF104-2

l

Cy7®

743

767

XF143-2

l

24

l l

l

l

l

l l

l


l

Ratio Imaging

l

l

Decoding filter descriptions

Pricing

Most filter descriptions are identified by center wavelength, filter design code and full width half maximum. For example, a 490DF20 filter has a 490-nm center wavelength, and a 20-nm bandwidth, or a 480- to 500-nm passband (all values are approximate). A 490DF20 passband is 490 ±10 nm, not 490 ±20 nm.

We provide the most competitive prices. For current product pricing, please visit www.glenspectra.cO.uk or contact us at: 

+44 020 8204 9517

Fluorophores

Fluorophores

(C-G)

Fluorophore


 i[email protected]

(G-M)

EX

EM

Best Set

Stock Standard

Cy3®/Cy5.5® FRET

550

694

XF167

Cy Pet

435

477

XF114-2

EX

EM

Best Set

eGFP/Rhod-2 FRET

488

571

XF151-2

HcRed

591

613

XF102-2

l

Cycle 3 GFP

395/478 507

XF76

Dansyl cadaverine

335

518

XF02-2

l

HiLyte Fluor™ 488

497

525

XF100-2

l

l

HiLyte Fluor™ 555

550

566

XF108-2

Dansylchloride

380

475

XF10-2

l

l

HiLyte Fluor™ 647

649

672

XF140-2

DAPI

358

461

QMAX-Blue

l

HiLyte Fluor™ 680

688

700

XF141-2

Dapoxyl®

373

574

XF05-2

l

l

HiLyte Fluor™ 750

750

782

XF143-2

DiA (4-Di-16-ASP)

491

613

XF21

l

l

Hoechst 33342 & 33258

352

461

QMAX-Blue

l

DiD (DilC18(5))

644

665

QMAX-Far Red

DIDS

341

414

XF03

7-Hydroxy-4-methylcoumarin (pH 9)

360

449

XF06

l

1,5 IAEDANS

336

482

XF02-2

Dil (DiIC18(3))

549

565

XF108-2

l

l

Indo-1

330

401

XF07

DiO (DiOC18(3))

484

501

XF100-2

l

l

JC-1

498/593 525/595 XF115-2

l

DiR (DiIC18(7))

750

779

XF143-2

Di-4 ANEPPS

488

605

XF21

l

6-JOE

525

555

XF104-2

l

l

JOJO™-1, JO-PRO™-1

529

545

XF104-2

Di-8 ANEPPS

468

635

XF21

l

l

JRed

584

610

QMAX-Red

DM-NERF (4.5–6.5 pH)

510

536

XF104-2

l

l

Keima Red

440

620

XF177

FRET

l

l

l l

Fluorophore

Stock Standard FRET

l

DsRed2 (Red Fluorescent Protein)

558

583

QMAX-FITC/TRITC

l

Kusabira Orange

548

559

QMAX-Yellow

l

DsRed-Express

557

579

XF108-2

l

Lissamine rhodamine B

570

590

XF102-2

l

DsRed Monomer

556

586

XF108-2

l

LOLO™-1, LO-PRO™-1

565

579

XF39

l

ELF® -97 alcohol

345

530

XF09

l

Lucifer Yellow

428

536

XF14-2

l

Emerald

487

509

XF172

l

LysoSensor™ Blue (pH 5)

374

424

XF131

l

EmGFP

487

509

XF100-2

l

LysoSensor™ Green (pH 5)

442

505

XF100-2

Eosin

524

544

XF104-2

l

LysoSensor™ Yellow/Blue (pH 4.2)

384

540

XF129-2

Erythrosin

529

554

XF104-2

l

LysoTracker® Green

504

511

XF104-2

l

Ethidium bromide

518

605

XF103-2

l

LysoTracker® Red

577

592

QMAX-Red

l

Ethidium homodimer-1 (EthD-1)

528

617

XF103-2

l

LysoTracker® Yellow

465

535

XF18-2

l

Europium (III) Chloride

337

613

XF02-2

l

Mag-Fura-2

330

491

XF04-2

l

5-FAM (5-Carboxyfluorescein)

492

518

XF100-2

l

Mag-Indo-1

330

417

XF07

l

Fast Blue

365

420

XF06

l

Magnesium Green™

506

531

XF104-2

l

Fluorescein (FITC)

494

518

XF100-2

l

Marina Blue®

365

460

XF06

l

FITC/Cy3® FRET

494

570

XF162

FRET

mBanana

540

553

CALL

FITC/Rhod 2 FRET

494

571

XF162

FRET

mCherry

587

610

QMAX-Red

l

FITC/TRITC FRET

494

580

XF163

FRET

mCitrine

516

529

XF104-2

l

Fluo-3

506

526

XF104-2

l

4-Methylumbelliferone

360

449

XF06

l

Fluo-4

494

516

XF100-2

l

mHoneydew

487

537

CALL

FluorX®

494

519

XF100-2

l

Midorishii Cyan

472

495

XF116-2

Fluoro-Gold™ (high pH)

368

565

XF09

l

Mithramycin

395

535

XF14-2

l

Fluoro-Gold™ (low pH)

323

408

XF05-2

l

Mitofluor Far Red

680

650-773 XF142-2

l

Fluoro-Jade

475

525

XF100-2

l

Mitofluor Green

490

516

XF100-2

l

FM® 1-43

479

598

XF115-2

l

Mitofluor Red 589

588

622

XF102-2

l

Fura-2

335

505

XF04-2

Mitofluor Red 594

598

630

XF102-2

l

Fura-2/BCECF

335/503 505/528 XF79-2

Ratio Imaging

MitoTracker® Green

490

516

XF100-2

l

Fura Red™

436

XF17

Ratio Imaging

MitoTracker® Orange

551

576

XF108-2

l

Fura Red™/Fluo-3

472/506 672/527 XF26

Ratio Imaging

MitoTracker® Red

578

599

XF102-2

l

GeneBLAzer™ (CCF2)

402

520

XF124

Ratio Imaging

MitoTracker® Deep Red

644

655

XF110-2

l

GFP wt

395/ 475 509

XF169

l

mOrange

548

562

XF108-2

l

eGFP

488

508

XF116-2

mPlum

590

649

XF110-2

l

GFP (sapphire)

395

508

XF76

l

mRaspberry

598

625

XF102-2

l

eGFP/DsRed FRET

470

585

XF151-2

FRET

mRFP

584

607

QMAX-Red

l

637

l

l


l l

l

l

l

25

Stock Filters



Products

Standard Filters



Fluorophores

Fluorophores

(S)

Fluorophore

(S-Z)

EX

EM

Best Set

EX

EM

Best Set

mStrawberry

574

596

XF174

mTangerine

568

585

XF108-2

SpectrumAqua®

433

480

XF201

SpectrumBlue®

400

450

XF06

mTFP

462

492

XF168

NBD

465

535

XF18-2

l

SpectrumGold®

530

555

XF203

MFISH

l

SpectrumGreen®

497

524

XF202

Nile Red

549

628

XF103-2

MFISH

l

SpectrumOrange®

559

588

XF204

Oregon Green® 488

496

524

XF100-2

MFISH

l

SpectrumRed®

587

612

XF207

Oregon Green® 500

503

522

MFISH

XF104-2

l

SpectrumFRed®

655

675

XF208

Oregon Green® 514

511

MFISH

530

XF104-2

l

SYTO® 11

508

527

XF104-2

l

Pacific Blue™ PBF1

410

455

XF119-2

l

SYTO® 13

488

509

XF100-2

l

334

504

XF04-2

l

SYTO® 17

621

634

XF44

C-phycocyanin

620

648

XF45

l

SYTO® 45

452

484

XF114-2

l

R-phycocyanin

618

642

XF102-2

l

SYTOX® Blue

445

470

XF114-2

l

R-phycoerythrin (PE)

565

575

XF108-2

l

SYTOX® Green

504

523

XF104-2

l

Phi YFP

525

537

XF104-2

l

SYTOX® Orange

547

570

XF108-2

l

PKH26

551

567

XF108-2

l

5-TAMRA (5-Carboxytetramethylrhodamine) 542

568

XF108-2

l

POPO™-1, PO-PRO™-1

434

456

XF114-2

l

tdTomato

554

581

XF173

POPO™-3, PO-PRO™-3

534

572

XF108-2

l

Tetramethylrhodamine (TRITC)

555

580

XF108-2

l

Propidium Iodide

536

617

XF103-2

l

Texas Red®/Texas Red®-X

595

615

XF102-2

l

PyMPO

415

570

XF15

l

Thiadicarbocyanine

651

671

XF47

l

Pyrene

345

378

XF02-2

l

Thiazine Red R

510

580

XF28

l

Pyronin Y

555

580

XF108-2

Thiazole Orange

453

480

XF114-2

l

Qdot™ 525 Conjugate

UV

525

XF301-1

Qdot

Topaz

514

527

XF104-2

l


UV

565

XF302-1

Qdot

T-Sapphire

399

511

XF76


UV

585

XF303-1

Qdot

TOTO®-1, TO-PRO®-1

514

533

XF104-2

l


UV

605

XF304-1

Qdot

TOTO®-3, TO-PRO®-3

642

660

XF110-2

l


UV

625

XF309-1

Qdot

TO-PRO®-5

748

768

XF143-2


UV

655

XF305-1

Qdot

Turbo RFP

553

574

XF108-2

l


UV

705

XF306-1

Qdot

Turbo YFP

525

538

XF104-2

l


UV

800

XF307-1

Qdot

Venus

515

528

XF104-2

l

Quinacrine Mustard

423

503

XF14-2

l

WW 781

605

639

XF45

Resorufin

570

585

XF102-2

l

X-Rhodamine (XRITC)

580

605

XF102-2

l

Red Fluorescent Protein (DsRed2)

561

585

XF108-2

l

YFP (Yellow Fluorescent Protein)

513

527

XF104-2

l

RH 414

500

635

XF103-2

YFP/Cy3® FRET

513

570

XF167

FRET

Rhod-2

550

571

XF108-2

l

YFP/TRITC FRET

513

580

XF166

FRET

Rhodamine B

555

580

XF108-2

l

YOYO®-1, YO-PRO®-1

491

509

XF100-2

l

Rhodamine Green™

502

527

XF104-2

l

YOYO®-3, YO-PRO®-3

612

631

XF102-2

l

Rhodamine Red™

570

590

XF102-2

l

Ypet

517

530

XF104-2

l

Rhodamine Phalloidin

542

565

XF108-2

l

ZsGreen1

493

505

XF100-2

l

Rhodamine 110

496

520

XF100-2

l

ZsYellow1

529

539

XF104-2

l

Rhodamine 123

507

529

XF104-2

l

5-ROX (carboxy-X-rhodamine)

574

602

XF102-2

l

SBFI

334

525

XF04-2

l

SensiLight P-1

550

664

XF36

l

SensiLight P-3

609

661

XF45

l

SITS

337

436

XF03

l

SNAFL®-1

576

635

XF29

Ratio Imaging

SNAFL®-2

525

546

XF29

Ratio Imaging

SNARF®-1

575

635

XF72

Ratio Imaging

Sodium Green™

507

535

XF104-2

26

Stock Standard l

l

l

l

Fluorophore

l


Stock Standard MFISH

l

l

l

l

l

l

Fluorescent Protein Sets 



Application High signal-to-noise imaging applications



Excitation Filters ALPHA bandpass designs



Design Features


ALPHA Technology excitation and emission filters provide steep edges, increased blocking, and reduced auto-fluorescence



Emission Filters ALPHA bandpass/longpass designs

Fluorescent Protein Sets Fluorophores


Hard-surface & AR coated

Filter Set #

Filter sets are arranged by fluorophores and emission wavelength. Application

Components

Type BFP QMAX-Blue

Wide exciter filter may cause cellular damage in live cell applications. Please inquire about excitation to reduce phototoxicity

BFP, eBFP XF129-2

Longpass emission filter will collect light Exciter from most of all UV excitable sources that Dichroic emit in the visible, including autofluorescence Emitter


Part #

Stock

Description


355-405 410LP 420-480

XF1075 XF2004 XF3088


l

l

l l l

BFP, eBFP XF131 Bandpass emission filter


XF1075 XF2004 XF3002

387AF28 410DRLP l 450AF65

CFP, eCFP, mCFPm, Cerulean, CyPet XF114-2 Bandpass emission filter


XF1071 XF2034 XF3075

440AF21 455DRLP 480AF30

Longpass emission filter is Exciter likely to have higher background Dichroic than bandpass emission filter Emitter

XF1071 XF2034 XF3087

l 440AF21 l 455DRLP 480ALP

CFP, eCFP, mCFPm, Cerulean, CyPet XF130-2

Standard

l l

l

l l l

l

wtGFP XF170 Longpass emission filter uses primary excitation peak


XF1076 XF2040 XF3087


l

AmCyan XF168


XF1101 XF2091 XF3119

440AF40 465DRLP 505AF50

l

eGFP, CoralHue Azami Green QMAX-Green Emerald



450-490 500LP 510-560

l

CoralHue Midoriishi-Cyan, eGFP XF116-2 For multiple labeling with DsRed2, Exciter Fluorescein (FITC) minimizing cellular auto-fluorescence Dichroic Alexa Fluor® 488, Cy2® Emitter

XF1072 XF2037 XF3080

475AF20 500DRLP 510AF23

l

eGFP XF115-2

XF1073 XF2010 XF3086


l


l l

l l

l l

l l

l l

wtGFP XF169 Bandpass emission filter uses primary excitation peak


XF1076 XF2040 XF3080

400AF30 435DRLP l 510AF23

l

CoralHue Azami Green XF171


XF1102 XF2027 XF3003

475AF30 485DRLP 520DF40

l

GFP (Sapphire), T-Sapphire, GFPuv XF76 UV Excitation, green emission color


XF1008 XF2006 XF3003

405DF40 450DCLP 520DF40

l


XF1068 XF2030 XF3082

l 500AF25 l 525DRLP 530ALP

YFP XF105-2

Emerald XF172


XF1072 XF2077 XF3084

475AF20 500DRLP 535AF45

l

ZsGreen1, eGFP, Fluorescein (FITC) XF100-2 Alexa Fluor® 488 Cy2®, DiO, Fluo-4


XF1073 XF2010 XF3084

475AF40 505DRLP 535AF45

l

eGFP XF100-3


XF1087 XF2077 XF3105

470AF50 500DRLP 545AF75

l

YFP, ZsYellow1 XF104-2 Bandpass emission filter


XF1068 XF2030 XF3074

500AF25 525DRLP 545AF35

l


l

l l

l l

l

l l

l l

l l

l l

27

Fluorescent Protein Sets

Products





High signal-to-noise imaging applications 

Fluorophores

Filter Set #

Dichroic Beamsplitters Hard-surface & AR coated



Emission Filters ALPHA bandpass/longpass designs

Filter sets are arranged by fluorophores and emission wavelength. Application

Components Type

DsRed2, DsRed-Express XF101-2



Design Features ALPHA Technology excitation and emission filters provide steep edges, increased blocking, and reduced auto-fluorescence

Fluorescent Protein Sets

Excitation Filters ALPHA bandpass designs

Application


Part #

Stock

Standard

Description

XF1074 XF2017 XF3085


• • • • • •

QMAX-Yellow Emission provides a DsRed2, mTangerine yellow-orange color to Ds Red2, TRITC and others



510-560 560LP 570-600

XF111-2 DsRed2, DsRed-Express


XF1077 XF2015 XF3089


XF108-2 Bandpass emission filter CoralHue Kusabira Orange DsRed2, DsRed-Express mOrange, mTangerine


XF1074 XF2017 XF3083

525AF45 560DRLP 595AF60

XF173 tdTomato


XF1103 XF2015 XF3083

535AF30 570DRLP 595AF60 •

Hc Red, mCherry, JRed QMAX-Red



530-570 580LP 600-650

AsRed 2, mStrawberry XF174


XF1104 XF2018 XF3120

555AF30 580DRLP 610AF50

• • •

XF176 NEW! Long pass emission filter Keima Red to give a rich red color in single label schemes


XF1071 XF2019 XF3094

440AF21 • 590DRLP 610ALP

• •

Keima Red XF177 NEW! Same excitation and emission filter as Exciter XF176, but blue shifted dichroic Dichroic mirror, multicolor applications Emitter

XF1071 XF2007 XF3094

440AF21 • 475DCLP 610ALP

• •

XF178 NEW! Bandpass version of XF177, Keima Red best for multi-label applications with other blue excited fluorophores


XF1071 XF2007 XF3025

440AF21 • 475DCLP 615DF45

• •

mCherry XF175


XF1067 XF2020 XF3081

560AF55 • 600DRLP 645AF75 •

HcRed, HcRed1, mRaspberry, MRFP1 XF102-2


XF1067 XF2029 XF3081

560AF55 595DRLP 645AF75

• • •

mPlum XF110-2


XF1069 XF2035 XF3076

630AF50 650DRLP 695AF55

• • •

Longpass emission filter, brighter signal, but potentionally higher background than bandpass emission filter

Red emission and good discrimination from eGFP in co-expression systems

Fluorescent Protein Multi-band Sets Fluorophores

Filter Set #

• • •

• • • • •

• • •

•


Application

Components Type

Part #

Stock

Standard

Description

BFP/eGFP XF50 Dual Band


XF1048 XF2041 XF3054

400-500DBEX 385-502DBDR 460-550DBEM

• • •

CFP/YFP XF135 Dual Band


XF1078 XF2065 XF3099

436-510DBEX 436-510DBDR 475-550DBEM

• • •

eGFP/DsRed2 XF52 Dual Band


XF1050 XF2043 XF3056

490-550DBEX 490-550DBDR 520-580DBEM

• • •

CFP/YFP/DsRed2 XF154 Triple Band


XF1100 XF2090 XF3118

436-495-582TBEX 445-510-600TBDR 465-535-640TBEM

• • •

28


FRET Sets 



Application Determining physical proximity in a sample



Excitation Filters Bandpass designs





Hard-surface & AR coated

Design Features Multiple emission filters



Emission Filters Bandpass designs

FRET Sets


Fluorophores Donor

Filter Set # Acceptor

Components Type

Part #

Stock

Standard

Description

BFP eGFP XF89-2

Exciter Dichroic Emitter 1 Emitter 2

XF1005 XF2001 XF3002 XF3084

365WB50 400DCLP 450AF65 535AF45

BFP YFP XF158


XF1005 XF2001 XF3002 XF3079

365WB50 • 400DCLP • 450AF65 • 535AF26

•

BFP DsRed2 XF159


XF1005 XF2001 XF3002 XF3019

365WB50 • 400DCLP • 450AF65 • 605DF50

•

CFP YFP XF88-2


XF1071 XF2034 XF3075 XF3079

440AF21 • 455DRLP • 480AF30 • 535AF26 •

CFP DsRed2 XF152-2


XF1071 XF2034 XF3075 XF3022

440AF21 • 455DRLP • 480AF30 • 580DF30 •

Midoriishi Cyan Kusabira Orange XF160


XF1071 XF2027 XF3005 XF3302

440AF21 • 485DRLP 495DF20 565WB20

eGFP DsRed2 or Rhod-2 XF151-2


XF1072 XF2077 XF3080 XF3083

475AF20 500DRLP 510AF23 595AF60

FITC TRITC XF163


XF1073 XF2010 XF3017 XF3083

475AF40 • 505DRLP • 530DF30 595AF60 •

FITC Rhod-2 or Cy3 XF162


XF1073 XF2010 XF3007 XF3083

475AF40 • 505DRLP • 535DF35 595AF60 •

Alexa 488 Alexa 546 or 555 XF164


XF1087 XF2077 XF3084 XF3083

470AF50 500DRLP • 535AF45 • 595AF60 •

Alexa 488 Cy3 XF165


XF1073 XF2010 XF3084 XF3083

475AF40 505DRLP 535AF45 595AF60

•

YFP TRITC or Cy3 XF166


XF1068 XF2030 XF3074 XF3083

500AF25 525DRLP 545AF35 595AF60

•

Cy3 Cy5 or Cy5.5 XF167


XF1074 XF2017 XF3083 XF3076

525AF45 560DRLP 595AF60 695AF55

•


• • • •

• • •

• • • •

•

• •

• • • • • • • • •

29

Overview

Application Note

Optimizing Filter Sets for FRET Applications FILTERS & MICROSCOPE CONFIGURATIONS The filter components required for FRET experiments are not esoteric. As in any fluorescence microscopy application, an excitation filter is required for exciting the donor fluorophore, and a dichroic mirror is required for separating donor excitation energy from both donor and acceptor emission energy. Unlike other fluorescence applications, however, two emission filters are required, one for the acceptor fluorophore, or FRET emission, and one for the donor fluorophore in order to correct for SBT. As for choosing specific filters, the same filter components and sets can be applicable for FRET as those which are matched to specific fluorophores and used in other single color, epi fluorescence applications. More important in the selection of filters is an understanding of the physical configuration of the microscope hardware to be used in the FRET experiment. At issue are critical experimental variables, such as time and image registration. While the ideal set-up may not be affordable or available to all researchers interested in FRET studies, it is nonetheless important to understand the pros and cons of the available hardware and filter set configurations.

1. Multi-View Configurations Most ideal for the viewing and measurement of molecular, proteinprotein interactions with critical spatial and temporal characteristic is a set-up which allows for simultaneous viewing of both donor and acceptor emission energy. This is only possible using a device which provides a simultaneous split-screen view of the sample. These multiview accessories are mounted to the microscope in front of the detector and use filters integrated into the unit to split the donor and acceptor emission fluorescence into two images. When FRET viewing is handled this way, the two critical variables— time and registration—are eliminated. The time of donor and acceptor imaging is simultaneous, and given a properly aligned unit, the image registration is identical, providing a duplicate view of the sample. The only difference between the two images is that one image is captured with an emission filter for donor emission while the other image uses an emission filter for acceptor emission.

2. Emission Filter Wheels When multi-view accessories are not available, an automated emission filter wheel is the next best alternative. With this configuration, a filter cube/holder with a donor excitation filter and dichroic mirror are placed in the microscope. The emission filters for both the donor and acceptor fluorophores, in turn, are mounted in the emission filter wheel, which can be rapidly switched from one to the other.

30

FRET, or Forster Resonance Energy Transfer, is a phenomenon where closely matched pairs of fluorophores are used to determine spatial or temporal proximity and specificity in molecular, proteinprotein interactions. More specifically, this energy transfer occurs when the emission energy of one fluorophore—the donor—is nonradiatively transferred to the second fluorophore—the acceptor— producing a secondary emission. When this occurs, donor fluorescence is quenched and acceptor fluorescence increases. Biologically, in order for this transfer to occur, the cellular conditions need to be such that the distance between the molecules being measured is no more than 1-10nm. Spectrally, the fluorophores being used need to have a large overlap, which while creating the conditions for effective energy transfer, also results in spectral bleed through (SBT), defined as the overlap of the donor and acceptor emission spectra, and can be a problem in FRET measurements. The development of SBT correction techniques have been critical to the evolution of FRET as a useful and more widely used application. These SBT correction techniques—which include software development, fluorescence lifetime imaging (FLIM) correlation, and photo bleaching techniques—have reached a degree of sophistication that improves the efficacy of FRET. Similarly, the development of microscopy techniques such as one-photon, two-photon (multi-photon), confocal, and TIRF are all contributing to the growing effectiveness and ease of FRET experiments. While much has been written about the physical and biological aspects of FRET, as summarized above, this application note will review the best suited fluorophore pairs and summarize the considerations surrounding the hardware configuration and selection of optical filters required for successful capture, differentiation, and measurement of FRET.

Collecting donor and acceptor emission energy using this hardware configuration, while not simultaneous, can be accomplished with time delays of only 40-75msec (depending on make and model), given the state-of-the-art of automated filter wheels as well as camera and detector technology. Both temporal changes in the sample during live cell imaging and registration shift resulting from equipment movement, while almost negated, must still be considered when analyzing experimental results.

3. Separate Filter Cubes Without a multi-view attachment or emission filter wheel, researchers must fall back on a third hardware configuration for FRET, which involves using a separate filter cube for both donor and acceptor fluorescence. In this configuration, while each cube has an exciter, dichroic and emitter, it is extremely important to remember that the exciter and dichroic in both sets are identical and are those filters that are typically used with the donor fluorophore. While this third filter configuration allows for the discreet collection of donor and acceptor fluorescence, it is the configuration most susceptible


to the time and image registration variables mentioned previously. The time variable can be minimized as a result of the automated turrets, which are a standard feature on many new microscopes but may not be typical on older, installed models. In addition, alignment of filters within cube tolerances allows more room for registration error than in the two other configurations. The time and resolution variables that are inherent with this configuration must be thoughtfully weighed when using a spatially and temporally sensitive technique such as FRET.

FLUOROPHORE PAIRS While certain fluorophore pairs such as CFP/YFP, have dominated the scientific literature and provided the foundation for successful FRET studies to date, there has been a flurry of activity recently surrounding the development of new monomeric fluorescent proteins which have applicability for FRET experiments. These fluorophore developments have been stimulated by the refinement of procedures and ratio correction techniques, as well as microscopy applications that are FRET friendly.

FRET FILTER SETS

The products listed in the catalog include the most commonly used FRET fluorophore pairs, as well as those recently developed pairs that are worthy of attention. For each FRET fluorophore pair the chart lists a filter set that is useful in the emission filter wheel configuration. These sets are comprised of the exciter and dichroic for the donor fluorophore and emitters for both the donor and acceptor fluorophores. Individual filter set part numbers for both donor and acceptor fluorophores are also listed so components can be purchased individually, dependent on the specifics of the hardware set-up. If purchasing filters individ ually, it is important to remember that the exciter and dichroic from the acceptor fluorophore set are never used. It is important that you provide hardware details and related mounting instructions when ordering filters for FRET applications

On the most basic level, the success of any given pair of fluorophores centers on their spectral characteristics. First, there must be sufficient separation of excitation spectra for selective stimulation of the donor. Second, there must be sufficient overlap (>30%) between the emission spectrum of the donor and the excitation spectrum of the acceptor in order to obtain efficient energy transfer. And third, there must be sufficient separation of the donor and the acceptor emission spectra so that the fluorescence of each fluorophore can be collected independently. Development of new fluorescent proteins has centered on meeting these criteria, while producing new colors and fluorophores that bind to varied proteins and biological molecules. The newest developments are cited in the links and references to recent literature listed below:


t Op

al ic

Ome ga

Fluorophore References Wallrabe, H., and Periasamy, A. (2005) FRET-FLIM microscopy and spectroscopy in the biomedical sciences. Current Opinion in Biotechnology. 16: 19-27. Karasawa, S., Araki, T., Nagai, T., Mizuno, H., Miyawaki, A. (2004) Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Biochemical Journal, April 5. Shaner, N., Campbell, R., Steinbach, P., Giepmans, B., Palmer, A., Tsien, R. (2004) Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology, Vol. 22, Number 12, December. pp.1567-1572.

31

Products

Quantum Dot Filters 



Application For imaging all UV-excited QDot™ conjugates





Design Features

Sets include a choice of two exciters, a single dichroic, and emitters optimized for each QDot

Quantum Dot (Qdot™) Filters & Filter Sets Fluorophores

Excitation Filters

Wide-band excitation filter for maximum signal or narrower bandpass design to minimize photodamage


Single dichroic functions for all colors 

Emission Filters

Bandpass designs for high signal-to-noise and discrimination, are AR coated and precision polished


Filter Set #

Components Type

Part #

Stock Standard Description

Qdot™ All Conjugates XF300

Exciter 1 Exciter 2 Dichroic Emitter 1 Emitter 2 Emitter 3 Emitter 4 Emitter 5 Emitter 6 Emitter 7 Emitter 8 Emitter 9

XF1009 XF1301 XF2007 XF3301 XF3302 XF3303 XF3304 XF3305 XF3113 XF3307 XF3308 XF3009

425DF45 415WB100 475DCLP 525WB20 565WB20 585WB20 605WB20 655WB20 710AF40 800WB80 840WB80 625DF20

l

Qdot™ 525 Conjugate XF301-1 or XF301-2 (Substitute Exciter 2 for Exciter 1)

Exciter 1 Exciter 2 Dichroic Emitter 1

XF1009 XF1301 XF2007 XF3301

425DF45 415WB100 475DCLP 525WB20

l



XF1009 XF1301 XF2007 XF3302

425DF45 415WB100 475DCLP 565WB20

l



XF1009 XF1301 XF2007 XF3303

425DF45 415WB100 475DCLP 585WB20

l



XF1009 XF1301 XF2007 XF3304

425DF45 415WB100 475DCLP 605WB20

l


XF1009 XF1301 XF2007 XF3009

425DF45 415WB100 475DCLP 625DF20

l



XF1009 XF1301 XF2007 XF3305

425DF45 415WB100 475DCLP 655WB20

l



XF1009 XF1301 XF2007 XF3113

425DF45 415WB100 475DCLP 710AF40

l

Qdot™ 800 Conjugate XF307-1 For single color or XF307-2 (Substitute Exciter 2 for Exciter 1)


XF1009 XF1301 XF2007 XF3307

425DF45 415WB100 475DCLP 800WB80

l

Qdot™ 800 Conjugate XF308-1 For multiplexing or XF308-2 (Substitute Exciter 2 for Exciter 1)


XF1009 XF1301 XF2007 XF3308

425DF45 415WB100 475DCLP 840WB80

l


32

XF309-1 NEW! or XF309-2 NEW! (Substitute Exciter 2 for Exciter 1)


l l l l l l l l l l l

l l l

l l l

l l l

l l l

l l l

l l l

l l l

l l l

l l l


Pricing


We provide the most competitive prices. For current product pricing, please visit www.glenspectra.co.uk or contact us at:


 

+44 020 8204 9517

Quantum Dot (Qdot™) Filters & Filter Sets Fluorophores

Filter Set #

Components Type

Part #

Stock

Standard

l

Description

For simultaneous XF320 multi-color viewing to minimize DAPI


XF1009 XF2007 XF3086

425DF45 475DCLP 510ALP

For simultaneous XF02-2 multi-color viewing with Xenon excitation


XF1001 XF2001 XF3097


For simultaneous XF05-2 multi-color viewing with Hg excitation


XF1005 XF2001 XF3097


l

l

l

l

l

l l

l

Multi-Q-Dot Emissions

Transmission (%)

100 –

XF1009 90 – XF2007 XF3301 80 – XF3302 XF3303 70 – XF3304 XF3305 60 – XF3307 50 – 40 – 30 – 20 – 10 – 0– 400

600

800

1000

Wavelength (nm)


33

Products

Pinkel Sets 

Application





Excitation Filters



Fluorophores

Emission Filters

Multi-band designs with multiple passbands and reflection bands

Multiple filters must be mounted in a filter wheel or slider

Pinkel Sets


Multi-band designs with multiple passbands and rejection bands

For multi-color high discrimination applications


Components Type

Part #


Dual Band DAPI/FITC XF50-1 BFP/eGFP

Exciter 1 Exciter 2 Dichroic Emitter

XF1006 XF1099 XF2041 XF3054

400DF15 485DF20 385-502DBDR 460-550DBEM

l

FITC/TRITC XF52-1 Cy2®/Cy3® eGFP/DsRed2


XF1099 XF1043 XF2043 XF3056

485DF20 555DF10 490-550DBDR 520-580DBEM

l

FITC/Texas Red® XF53-1


XF1099 XF1044 XF2044 XF3057

485DF20 575DF25 490-575DBDR 528-633DBEM

l

DAPI/TRITC XF59-1


XF1094 XF1045 XF2047 XF3060

380AF15 560DF15 395-540DBDR 470-590DBEM

l

CFP/YFP XF135-1


XF1079 XF1080 XF2065 XF3099

436DF10 510DF25 436-510DBDR 475-550DBEM

l

Triple BanD DAPI/FITC/Texas Red® XF63-1

Exciter 1 Exciter 2 Exciter 3 Dichroic Emitter

XF1006 XF1099 XF1044 XF2048 XF3061

400DF15 485DF20 575DF25 400-477-575TBDR 445-525-650TBEM

l

DAPI/FITC/Texas Red® XF67-1 DAPI/Alexa Fluor® 488/546 DAPI/Cy2®/Cy3®


XF1006 XF1099 XF1044 XF2045 XF3058

400DF15 485DF20 575DF25 400-485-580TBDR 457-528-633TBEM

l

DAPI/FITC/TRITC XF68-1 DAPI/FITC/Cy3®


XF1006 XF1099 XF1045 XF2050 XF3063

400DF15 485DF20 560DF15 385-485-560TBDR 460-520-602TBEM

l

DAPI/FITC/MitoTracker Red XF69-1


XF1006 XF1099 XF1044 XF2051 XF3116

400DF15 485DF20 575DF25 400-495-575TBDR 470-530-620TBEM

l

FITC/Cy3®/Cy5® XF93-1 FITC/TRITC/Cy5®


XF1099 XF1043 XF1046 XF2054 XF3067

485DF20 555DF10 655DF30 485-555-650TBDR 515-600-730TBEM

l

CFP/YFP/DsRed2 XF154-1


XF1201 XF1099 XF1044 XF2090 XF3118

436AF8 485DF20 575DF25 455-510-600TBDR 465-535-640TBEM

l

34


l l l

l l l

l l l

l l l

l l l

l l l l

l l l l

l l l l

l l l l

l l l l

l l l l

Sedat Sets 



Application

For multi-color imaging 


Stationary dichroic must be mounted in a filter cube. 

Excitation Filters


Emission Filters

Pinkel Sets Continued Fluorophores


Components Type

Quad BanD DAPI/FITC/TRITC/Cy5® XF57-1

Part #

Exciter 1 Exciter 2 Exciter 3 Exciter 4 Dichroic Emitter

Sedat Sets Fluorophores


Multiple single band filters in automated filter wheel

XF1006 XF1099 XF1045 XF1046 XF2046 XF3059

Stock Standard Description 400DF15 485DF20 560DF15 655DF30 400-485-558-640QBDR 460-520-603-710QBEM

l l l l l l


Dual BanD FITC/ TRITC XF156

Components

Type Exciter 1 Exciter 2 Dichroic Emitter 1 Emitter 2

Part #


XF1042 XF1043 XF2043 XF3084 XF3024

485DF15 555DF10 490-550DBDRLP 535AF45 590DF35

Triple BanD DAPI/FITC/TRITC XF157

Exciter 1 Exciter 2 Exciter 3 Dichroic Emitter 1 Emitter 2 Emitter 3

XF1006 XF1009 XF1045 XF2050 XF3002 XF3084 XF3025

400DF15 485DF20 560DF15 400-485-580TBDR 450AF65 535AF45 615DF45

Quad BanD XF155 DAPI/FITC/TRITC/Cy5 DAPI/FITC/TRITC/ Alexa Fluor®647

Exciter 1 Exciter 2 Exciter 3 Exciter 4 Exciter 5 Dichroic Emitter 1 Emitter 2 Emitter 3 Emitter 4

XF1005 XF1006 XF1099 XF1045 XF1208 XF2046 XF3002 XF3084 XF3024 XF3076

365WB50 400DF15 485DF20 560DF15 640AF20 400-485-558-640QBDR 450AF65 535AF45 590DF35 695AF55

l l l

l

l

l l l l

l l

l

l

l l l l l

l l

l

l

Photoswitchable Protein Set Fluorophores

Filter Set #

Components Type

Green to Red Conversion of XF153 Kaede, Eos, Kikumi

Exciter Red Exciter Green Conversion - Exciter UV R to G Dichroic Conversion - Dichroic UV R to G Emitter Red Emitter Green

Part # XF1072 XF1021 XF1401 XF2038 XF2001 XF3017 XF3089

Stock Standard Description 475AF20 550DF30 380DF30 430DRLP 400DCLP 530DF30 575ALP

l l l l l l l

The set includes seven filters which allows for both green and red visualization, as well as UV conversion. This technique is useful in live cell applications as an optical marking technique for studying cell dynamics.


35

Products

M-FISH Sets 



For maximizing multi-color labeling 



Design Features

Filter edges are steep and bandwidth is narrow allowing maximization of both emission energy and color discrimination

Excitation Filters

Narrow bandpass designs to maximize discrimination

Application


Steep transition designs, hard-surface and AR coated 

Emission Filters

Narrow bandpass designs to maximize discrimination

M-FISH Fluorophores


Components Type

Part #

Stock

Standard

Description

DAPI, AMCA, Cascade Blue® XF06 SpectrumBlue®


XF1005 XF2001 XF3002

365WB50 400DCLP 450AF65

l

DAPI/FITC/Texas Red® XF232


XF1005 XF1006 XF1099 XF1044 XF2045 XF3058

365WB50 400DF15 485DF20 575DF25 385-485-560TBDR 457-528-633TBEM

l

DAPI/FITC/TRITC XF231


XF1005 XF1006 XF1099 XF1045 XF2050 XF3063

365WB50 400DF15 485DF20 560DF15 400-485-580TBDR 460-520-602TBEM

l

SpectrumAqua®, CFP, DEAC XF201


XF1201 XF2034 XF3075

436AF8 455DRLP 480AF30

l

SpectrumGreen®, FITC, EGFP, Cy2® XF202 Alexa Fluor® 488, Oregon Green® 488 Rhodamine GreenTM


XF1202 XF2010 XF3017

485AF20 505DRLP 530DF30

l

l

SpectrumGold®, Alexa Fluor® 532 XF203 YFP


XF1203 XF2203 XF3302

520AF18 545DRLP 565DF20

l

Cy3®, TRITC, Alexa Fluor® 546 XF204 5-TAMRA, BODIPY® TMR/X SpectrumOrange®


XF1204 XF2062 XF3022

546AF10 555DRLP 580DF30

l

Cy3.5® XF206


XF1206 XF2019 XF3020

572AF15 590DRLP 620DF35

l

SpectrumRed®, Texas Red® XF207 Alexa Fluor® 568, BODIPY® TR/X Alexa Fluor® 594


XF1207 XF2020 XF3028

580AF20 600DRLP 630DF30

l

Cy5®, BODIPY® 650/665-X XF208 Alexa Fluor® 647


XF1208 XF2035 XF3031

640AF20 650DRLP 682DF22

l

l

l

Cy5.5® XF210


XF1210 XF2082 XF3112

665AF32 692DRLP 710AF40

l

Cy7® XF143-2


XF1097 XF2084 XF3115


l

36


l l

l l l l l

l

l

l l l l

l

l

l l

l l

l l

l l

l l

l l

Ratio Imaging Sets 

Application Ratiometric measurements of dyes



esign Features D Multiple excitation and multiple emission filter sets



Excitation Filters Bandpass designs



Dichroic Beamsplitters Hard-surface and AR coated



mission Filters E Bandpass designs

Ratio Imaging Sets Fluorophores



Components Type

Part #


Single Dye Excitation Sets Fura-2, Mag-Fura-2 XF04-2 PBFI, SBFI


XF1093 XF1094 XF2002 XF3043

340AF15 380AF15 415DCLP 510WB40

BCECF XF16


XF1071 XF1011 XF2058 XF3011

440AF21 490DF20 515DRLPXR 535DF25

l

Fura Red™ XF17


XF1071 XF1011 XF2009 XF3012

440AF21 490DF20 550DCLP 660DF50

l

l

SNAFL®-1, SNAFL®-2 XF29


XF1080 XF1017 XF2011 XF3020

510DF25 555DF27 585DRLP 620DF35

l

BTC XF133


XF1006 XF1099 XF2061 XF3011

400DF15 485DF20 400-485DBDR 535DF25

l

Single Dye Emission Sets Indo-1, Mag-Indo-1 XF07 LysoSensor™ Yellow/Blue (pH 4.2)

Exciter Dichroic 1 Dichroic 2 Emitter 1 Emitter 2

XF1004 XF2003 XF2006 XF3004 XF3005

355HT15 390DRLP 450DCLP 405DF43 495DF20

l

BODIPY® FL-Ceramide, JC-1 XF26


XF1099 XF2010 XF3016 XF3017

485DF20 505DRLP 0G590 530DF30

l

SNARF®-1 XF31 Confocal


XF1018 XF2013 XF2014 XF3022 XF3023

SNARF®-1 XF72 Widefield


GeneBLAzer™ (CCF2) XF124 Dual Dye Set Fura-2/BCECF XF79-2

l l l l

l l l

l l

l l l

l l l

l l l l

l

l

515NB2.0 540DCLP 610DRLP 580DF30 640DF35

l

XF1080 XF2013 XF2014 XF3022 XF3023

510DF25 540DCLP 610DRLP 580DF30 640DF35

l


XF1006 XF2059 XF3002 XF3007

400DF15 415DRLP 450AF65 535DF35

l


XF1093 XF1094 XF1071 XF1011 XF2031 XF3007

340AF15 380AF15 440AF21 490DF20 505DRLPXR 535DF35


l

l l l l

l l l l

l

l

l

l l

l

l l l

37

Multiband Sets

Products



Application



Simultaneous viewing of multiple fluorophores 

Design Features

Excitation & Emission Filters, as well as Dichroic Beamsplitters are multi-band designs with multiple passbands and rejection bands

Dual, triple & quad bandpass designs

Multi-band Sets Fluorophores


Filter Set #

Application

Components Type

Part #


Dual BanD DAPI/FITC XF50 BFP/eGFP


XF1048 XF2041 XF3054

400-500DBEX 385-502DBDR 460-550DBEM

l

DAPI/TRITC XF59


XF1054 XF2047 XF3060

390-520DBEX 395-540DBDR 470-590DBEM

l

CFP/YFP XF135


XF1078 XF2065 XF3099

436-510DBEX 436-510DBDR 475-550DBEM

l

FITC/TRITC XF52 eGFP/DsRed2


XF1050 XF2043 XF3056

490-550DBEX 490-550DBDR 520-580DBEM

l

FITC/APC XF51


XF1049 XF2042 XF3055

490-605DBEX 490-600DBDR 522-637DBEM

l

FITC/Texas Red® XF53


XF1051 XF2044 XF3057

490-577DBEX 490-575DBDR 528-633DBEM

l

Cy3®/Cy5® XF92


XF1062 XF2053 XF3066

550-640DBEX 555-640DBDR 595-700DBEM

l

Triple BanD Real time visual detection DAPI/FITC/Texas Red® XF63


XF1055 XF2048 XF3061

400-477-580TBEX 400-477-575TBDR 445-525-650TBEM

l

DAPI/FITC/Texas Red® XF56 Real time visual imaging with a CCD camera or color film


XF1052 XF2045 XF3058

390-486-577TBEX 400-485-580TBDR 457-528-633TBEM

l

DAPI/FITC/Texas Red® XF67 Real time visual detection


XF1058 XF2045 XF3058

390-486-577TBEX 400-485-580TBDR 457-528-633TBEM

l

DAPI/FITC/TRITC XF66 Real time visual imaging with a CCD camera or color film


XF1057 XF2050 XF3063

385-485-560TBEX 385-485-560TBDR 460-520-602TBEM

l

DAPI/FITC/TRITC XF68 Real time visual detection


XF1059 XF2050 XF3063

386-485-560TBEX 385-485-560TBDR 460-520-602TBEM

l

DAPI/FITC/Cy5® XF95


XF1065 XF2056 XF3069

400-510-630TBEX 390-510-630TBDR 460-560-690TBEM

l

DAPI/FITC/Propidium Iodide XF69


XF1098 XF2051 XF3116

400-495-575TBEX 400-495-575TBDR 470-530-620TBEM

l

DAPI/Cy3®/Cy5® XF94


XF1064 XF2055 XF3068

415-535-630TBEX 400-535-635TBDR 495-575-700TBEM

l

CFP/YFP/DsRed2 XF154


XF1100 XF2090 XF3118

436-495-582TBEX 445-510-600TBDR 465-535-640TBEM

l

FITC/Cy3®/Cy5® XF93


XF1063 XF2054 XF3067

485-555-650TBEX 485-555-650TBDR 515-600-730TBEM

l

Quad BanD DAPI/FITC/TRITC/Cy5® XF57 DAPI/FITC/TRITC/ Alexa Fluor®647


XF1053 XF2046 XF3059

405-490-555-650QBEX 400-485-558-640QBDR 460-520-603-710QBEM

l

38


l l

l l

l l

l l

l l

l l

l l

l l

l l

l l

l l

l l

l l

l l

l l

l l

l l

l l

Pricing We provide the most competitive prices. For current product pricing, please visit www.glenspectra.co.uk or contact us at:  

Emission Color Chart 350



excitation

emission

400

450

500

550

600

650

700

750

400

450

500

550

600

650

700

750

Dual BanD XF50 XF59 XF135 XF52 XF51 XF53 XF92

Triple BanD XF63 XF56 XF67 XF66 XF68 XF95 XF69 XF94 XF154 XF93

Quad BanD XF57

350


39

Products

Caged Compound Sets 



Application

UV excitation uncages fluorophores for controlled viewing 



Excitation Filters

Fluorophores

Emission Filters

Emission must be mounted in a filter holder


Caged Compound Sets


Extended reflection design. Dichroic beamsplitters must be mounted in a filter holder


Components Type

Stock

Part #

Standard

Description

FITC XF73-2

Uncaging Exciter Dye Exciter Dichroic Dye Emitter

XF1001 XF1073 XF2031 XF3084

330WB80 475AF40 505DRLPXR 535AF45

l

TRITC XF74-2


XF1001 XF1074 XF2032 XF3085

330WB80 525AF45 565DRLPXR 565ALP

l

FITC/TRITC XF75


XF1001 XF1050 XF2039 XF3056

330WB80 490-550DBEX 485-555DBDR 520-580DBEM

l

l l l

l l l

l l l

Extended Reflection Dichroic Only l FITC Extended Reflection Dichroic XF2031 505DRLPXR l TRITC Extended Reflection Dichroic XF2032 565DRLPXR l FITC/TRITC Dual Dichroic with UV Reflection XF2039 485-555DBDR

Multiphoton filters are used in conjunction with two- and three-photon IR laser excitation of fluorophores for imaging deeper into samples with minimal photobleaching and photodamage to cells.

Multi-Photon Filters

Components Type Dichroic Laser Blocking Filter

Part # XF2033 XF3100

Stock

Standard

Description 675DCSPXR 710ASP

Beamsplitters & Mirrors Part #

Description

XF121 XF122 XF123 XF125

50/50 Beamsplitter 70/30 Beamsplitter 30/70 Beamsplitter Reflecting Mirror

Neutral Density Filters

l l

Available in standard dichroic sizes and designed to function at 45 degree angle of incidence from 400–700nm. Application

Size

50%T, 50%R 70%T, 30%R 30%T, 70%R Opaque backing prevents transmission, >= 90% Reflection

Standard dichroic Standard dichroic Standard dichroic Standard dichroic

For reducing excitation energy.

Sizes

18Ø

25Ø

32Ø

45Ø

50Ø

50 x 50

Apertures Apertures:

13Ø

20Ø

27Ø

40Ø

45Ø

45 x 45

XB193/45R XB13/45R XB194/45R XB14/45R XB195/45R XB15/45R XB196/45R XB197/45R XB198/45R XB16/45R XB17/45R XB27/45R XB28/45R XB199/45R


XB193/50S XB13/50S XB194/50S XB14/50S XB195/50S XB15/50S XB196/50S XB197/50S XB198/50S XB16/50S XB17/50S XB27/50S XB28/50S XB199/50S

Description

Part #

ND 0.05 ND 0.1* ND 0.2 ND 0.3* ND 0.4 ND 0.5* ND 0.6 ND 0.7 ND 0.8 ND 1.0* ND 2.0* ND 3.0* Set of 6 - includes items with * Set of 12

40






Used to reduce infrared energy from the light source in the excitation path (XF83) or in front of the detector in the emission path (XF85 and XF86).

IR Blocking Filters Part #

Description



Application

Typical T%

Size

XF83 KG5 Blocks infrared energy at the light source 80% avg.

12R, 18R, 20R, 22R, 25R 32R, 45R, 50R, 50S

XF85 550CFSP 99+% Near IR Attenuation, 600-1200nm >75%T

12R, 18R, 20R, 22R, 25R 32R, 45R, 50R, 50S

XF86 700CFSP 99+% Near IR Attenuation, 750-1100nm >90%T

12R, 18R, 20R, 22R, 25R 32R, 45R, 50R, 50S

IR-DIC Filters Part #

Description

Used for simultaneous capture of fluorescence and infrared DIC images. Application

Size

XF117/32R

780DF35

Capture of fluorescence and infrared DIC images

32mm

XF117/45R

780DF35

Capture of fluorescence and infrared DIC images

45mm

Polarizing Filters Part #

Description

XF120

Polarizing Filter

Used to polarize incident light in both the excitation and emission path. Application Polarize light in both the excitation and emission path

Set of 4 slides

50 x 50 mm

This set of slides helps to: center and adjust the fluorescence illuminator; verify uniformity of fluorescence staining; monitor and adjust laser output and PMT settings; and avoid microspheres and photobleaching.

Fluorescence Reference Slides Part #

Size

Description

XF900

Blue emission

DAPI/Indo-1/Fura

Green emission

FITC/GFP

Yellow emission Acridine Orange

Red emission

Rhodamine/Texas Red®


41

Products

Microscope Cubes and Filter Holders Omega Optical offers holders or cubes for the most common fluorescence microscopes offered by Nikon, Olympus, Leica, and Zeiss. We can mount filters either in a factory supplied holder or a customer supplied holder.

Microscope Cubes and Filter Holders Type Omega Optical Part #

Manufacturer & Model

Nikon

Exciter

Emitter

XC100 Original (Labophot, Diaphot, Optiphot, Microphot, TMD, FXA) 18mm 18 x 26mm 18mm XC101 Modified (Labophot, Diaphot, Optiphot, Microphot) 20mm 18 x 26mm 22mm XC102 Quadfluor, Eclipse (E Models; TE 200/300/800; LV 150/150A/100D, Diaphot 200 & 300, Labophot 2 and Alphaphot 2 ) 25mm 25.7 x 36mm 25mm XC104 TE2000, Eclipse 50i, 80i, LV- series 25mm 25.7 x 36mm 25mm XC106 TE2000 plastic cube, compatible with AZ100 25mm 25.7 x 36mm 25mm Olympus XC110 IMT-2 22mm 21 x 29mm 20mm XC111 BH2 (cube style—not barrel, BHT, BHS, BHTU, AHBS 3, AHBT 3) 18mm 18 x 26mm 18mm XC113 BX2 (BX, IX, AX) 25mm 25.7 x 36mm 25mm XC114 CK-40 (CK Models 31/40/41, CB Models 40/41, CKX 31/41) 20mm 21 x 29mm 20mm XC115 SZX-FL Stereo Exciter Holder (Holds 1 exciter filter) 25mm N/A N/A XC116 SZX-FL Stereo Emission Holder (Holds 2 emission filters) N/A N/A 20mm Leica XC120 Ploemopak (DMIL, Diaplan, Dialux, Diavert, Fluovert, Labolux, Labovert, Orthoplan, Ortholux) 18mm 18 x 26mm 18mm XC121 DM (DML, DMR, DMLB, DMLM, DMLFS, DMLP) 22mm 21 x 29mm 22mm XC122 DMIRB (DMIL, DMRXA2, DMLS, DMICHB, DMLSP) 20mm 18 x 26mm 20mm XC123 DM2000, DM2500, DM3000, DM4000, DM5000, DM6000 22mm 21 x 29mm 22mm XC124 MZ FL III Stereo (Holds 2 emission filters) 18mm N/A 18mm Zeiss XC131 Axio Exciter Slider (for exciters or ND filters, 5 ports) 18mm N/A N/A XC132 Axioskop 2 Cube (Axioplan 2, Axioskop 2, Axiovert 25, Axioskop 2FS) 25mm 25.7 x 36mm 25mm XC133 Axiovert 3FL Slider 25mm 25.7 x 36mm 25mm XC134 Axioskop 4FL Slider (Axiovert 100/135, Axioplan 1, Axioskop 1, Axioskop FS 1) 25mm 25.7 x 36mm 25mm XC135 Axioskop 6FL Slider (Axiovert 100/135, Axioplan 1, Axioskop 1, Axioskop FS 1) 25mm 25.7 x 36mm 25mm XC136 Axio 2 Push-and-Click 25mm 25.7 x 36mm 25mm XC137 Axioskop 5FL Slider (Axiovert 100/135, Axioplan 1, Axioskop 1, Axioskop FS 1) 25mm 25.7 x 36mm 25mm XC138 Axioskop 8FL Slider 25mm 25.7 x 36mm 25mm XC139 Standard 2FL Slider (Axiovert 100/135, Axioplan 1, Axioskop 1, Axioskop FS 1) 25mm 25.7 x 36mm 25mm

Nikon XC106

Nikon XC104

Olympus XC111

Zeiss XC132

42

Dichroic

Olympus XC113

Leica XC121

Zeiss XC131


Leica XC122

Leica XC123

Zeiss XC136

Filters for Flow Cytometry Omega Optical offers flow cytometry filters to support a wide variety of fluorophores, tandems, and multi-color cell sorting and analysis applications. To meet the demands of the increasing number of lasers and fluorescent detection channels offered by current flow cytometers, the filters are designed for maximum multiple dye discrimination. Filters designed for use in flow cytometry must meet performance specifications suitable for laser applications. Dichroic mirrors used for beam directing/splitting are made of durable, hard, or semi-hard coatings for long lifetimes, and substrates can be ground and polished to meet parallelism specifications. Emission filters have precisely placed transmission bands and are designed to attenuate energy at the excitation laser line to optical density (OD) 5 or better. We work with researchers in the flow cytometry community to continually provide optimal filter selections for both standard methodologies and customized set-ups. Often the default filters supplied with the instrument are not optimal for discriminating between closely matched fluorophores, and a novel filter arrangement is required for the separation. For instance, three or four fluorescent proteins can be simultaneously detected using two lasers for excitation (407 and 488nm, or 413 and 514nm) and several narrow band discriminating filters for emission.1 See Figure 1 for filter set up for three (A) and four (B) fluorescent protein detection. 1

Methods in Molecular Biology: Flow Cytometry Protocols, 2nd ed. Chp 12. Edited by Hawley, T.S and Hawley, R.G. Humana Press Inc. 2004.

Figure 1A

Figure 1B

Dual Excitation Wavelengths 514 nm + 413 nm

Dual Excitation Wavelengths 488 nm + 407 nm

YFP

GFP 510/20

546/20

525 SP

YFP 550/30

610/20 560 SP

610 SP

DsRed

470/20 CFP

610/20 DsRed

470/20 3-way split mirror

CFP

3-way split mirror

514 nm primary 413 nm tertiary


488 nm primary 407 nm tertiary

43

Products v i o l e t U l t r a v i o l e t

excitation laser

Flow Cytometry Filters 




Size: 25, 15, and 12.5 mm. Shape: Specify round and/or square. Thickness: Ring 6.7 mm AOI: Specify dichroic AOI 45° or 11.25°

Flow Cytometry Emission Filters Dyes

Part #

355

Indo-1 (bound Ca 2+) Indo-1 (bound Ca 2+) DAPI, AMCA, Hoechst 33342 and 32580, Alexa Fluor® 350, Marina Blue®

XCY-390DF30 XCY-405DF20 XCY-424DF44

405

Cascade Blue®, Pacific Blue™, Hoechst 34580

XCY-440DF10 NEW! XCY-440DF40

Cascade Blue®, Pacific Blue™ DAPI, AMCA, Hoechst 33342 and 32580, Alexa Fluor® 350, Marina Blue ®, BFP Alexa Fluor® 405, Pacific Blue™ Chromomycin A3 Indo-1 (free Ca+2), CFP Indo-1 (free Ca+2), CFP Pacific Orange 405/488

XCY-450DF50 XCY-475ABLP XCY-485DF22 XCY-495DF20 NEW! XCY-550DF40

r e d b l u e

Filter created under collaboration with Dr. Tomas Kalina, Charles University, Prague.

Quantum Dot Emission Filters

GFP (for separation from YFP, also for separation from Qdots 545 and higher) GFP, FITC, Alexa Fluor® 488, Oregon Green® 488, Cy2®, ELF®-97, PKH2, PKH67, Fluo3/Fluo4, LIVE/DEAD Fixable Dead Cell Stain GFP, FITC, Alexa Fluor® 488, Oregon Green® 488, Cy2®, ELF-97, PKH2, PKH67, YFP YFP (for separation from GFP)

XCY-530DF30 XCY-535DF45 XCY-550DF30

XCY-630DF22 XCY-635DF40 NEW! XCY-660DF35 XCY-680AGLP

532

PE-Cy5.5®, PE-Alexa Fluor® 700

NEW! XCY-710DF40

633

APC, Alexa Fluor® 633, CF-1, CF-2, PBXL-1, PBXL-3 Cy5® (for separation from Cy7® and conjugates) Cy5®, Alexa Fluor® 647, TO-PRO-3

488 or 633

Cy5®, Alexa Fluor® 647, Alexa Fluor® 660, PE-Alexa Fluor® 647, PE-Cy5®, TO-PRO-3 Cy5.5®, Alexa Fluor® 680, PE-Alexa Fluor® 680, APC-Alexa Fluor® 680, PECy5.5® APC-Cy5.5® Cy7® (for separation from Cy5® and conjugates) PE-Cy7®, APC-Cy7® Cy7® Cy7®, APC-Alexa Fluor® 750

525WB20 565WB20 585WB20 605WB20 655WB20 715AF40

XCY-510DF21

PE, PI, Cy3®, CF-3, CF-4, TRITC, PKH26 Mithramycin PE, PI, Cy3®, CF-3, CF-4, TRITC, PKH26 Lissamine Rhodamine B, Rhodamine Red™, Alexa Fluor® 568, RPE-Texas Red®, Live/Dead Fixable Red Stain Lissamine Rhodamine B, Rhodamine Red™, Alexa Fluor® 568, RPE-Texas Red®, Live/Dead Fixable Red Stain RPE-Texas Red®, Texas Red®, Alexa Fluor® 594 PE-Alexa Fluor® 610 PE-Cy5® PE-Cy5®, PE-Cy5.5®, PE-Cy7®

633

Wider emission band for greater signal.

XCY-575DF26 XCY-575ABLP XCY-585DF22 XCY-610DF20 XCY-610DF30

XCY-660DF20 XCY-670DF40 XCY-675DF20 XCY-682DF22 XCY-710DF20 XCY-700DF20 XCY-740ABLP XCY-748ALP XCY-770ABLP XCY-787DF43

green

488 or 532

Description

XCY-450DF20

The 405 laser is optimal for excitation of Quantum Dots, but the 488 line laser can also be used. Qdot 525 XF3301 Qdot 565 XF3302 Qdot 585 XF3303 Qdot 605 XF3304 Qdot 655 XF3305 Qdot 705 XF3113 488

Emission Filters

Size: 25, 15, and 12.5 mm. Shape: Specify round and/or square. Thickness: Ring 6.7 mm Blocking: UV-900nm, OD5

44



Flow Cytometry Filter Sets Filter Set #

Laser

NEW!

405

XCY-502

457

NEW! XCY-503 XFY-500

NEW! XCY-504

NEW! XCY-505 XCY-501

457

488

488

488

633

Fluorophores CFP/YFP

CFP/GFP/YFP

EGFP/YFP

GFP/YFP/DsRed GFP/YFP DsRed/DsRed2 Cy5®/Cy7®



Flow Cytometry Dichroic Filters Type

Part #

Components

Description

Emitter 1

XCY-470DF20

XCY-380DRLP

Emitter 1

XCY-485DF22

XCY-425DRLP

Separation of DAPI from FITC and GFP

Emitter 2

XCY-550DF30

XCY-450DRLP

Separation of Cascade Blue® and Pacific Blue™ from FITC

Dichroic Emitter 1

XCY-505DRLPXR XCY-485DF22

XCY-460DRLPXR

Extended Reflection Longpass; Indo-1 ratiometric analysis (extended

Emitter 2

XCY-510DF21

Emitter 3 Emitter 1

XCY-550DF30 XCY-510DF21

Emitter 1

XCY-550DF30

Dichroic Emitter 1

XCY-525DCSP XCY-510DF21

Detection of side scatter during Indo-1 analysis

reflection) and Separation of Hoechst 33258 from Chrononycin A3 Separation of Cascade/Pacific Blue from Qdots and longer wavelength XCY-480DRLP

dyes to 700nm Separation of Cascade Blue® from Cascade Yellow® of ELF®-97

XCY-505DRLPXR

Extended Reflection Longpass; Reflects 451nm, 457nm, 477nm, 488nm and UV laser lines, Transmits > 525nm

Emitter 2

XCY-550DF30

XCY-525DCSP

Emitter 3 Emitter 1

XCY-610DF20 XCY-530DF30

XCY-560DRSP

Shortpass; Separation of FITC from PE

XCY-570DRLP

Separation of Mithramycin from Ethidium Bromide

Emitter 2

XCY-610DF20

XCY-610DRSP

Shortpass; Separation of FITC or PE from PE-Cy5®; Hoechst SP analysis

Dichroic Emitter 1

XCY-560DRSP XCY-670DF40

NEW! XCY-630DRLP

Separation of PE-Cy5® from Cy5.5® and Cy7® and their conjugates

Emitter 2

XCY-740ABLP

XCY-640DRLP

Separation of APC from dyes with shorter wavelength

Dichroic

XCY-710DMLP

Shortpass; Separation of GFP from YFP

NEW! XCY-680DRLP

Separation of PE-Cy5® and PE-Cy5.5

XCY-690DRLP

Separation of APC from APC-Cy5.5® or APC-Cy7®

XCY-710DMLP

Separation of PE and Cy5® from PE-Cy5.5® or PE-Cy7®

XCY-760DRLP

Separation of Cy5.5® from Cy7® and their conjugates

Application Note

Testing the optimal filters for Pacific Orange conjugates in polychromatic flow cytometry experiments. Tomas Kalina, CLIP – Cytometry Department of Paediatric Hematology/Oncology 2nd Medical School Charles University Prague, Czech Republic

Rationale

Currently used polychromatic flow cytometers (BD LSR2, Dako Cyan ADP, BD Canto 2) are equipped with two detectors for 405 nm laser excited fluorochromes. BD recommends using Pacific Blue and AmCyan conjugates, whereas Dako recommends Pacific Blue and Cascade Yellow conjugates for respective instruments. Both dyes for the 2nd Violet channel have their drawbacks: AmCyan dye, while the brightest, has significant spillover to the Pacific Blue, FITC and PE channel, thus after appropriate compensation it creates the signal of negative events. Cascade Yellow has no such unwanted spillover but is relatively dimmer than alternative dye Pacific Orange (Molecular Probes/Invitrogen). Based on previous comparison tests we selected Pacific Orange dye as a second violet dye for our nine (9) color experiments. Pacific Orange has peak emission at 551 nm. But the emission curve is rather wide so it can be measured in range of wavelengths from 510 nm to 620 nm. We initially found the detection at 585/42 nm slightly superior to 525/50 nm, mainly due to lower background. Surprisingly, we detected contaminating signal from FITC and from PE.

Methods

We measured single stained tubes with CD4 and CD8 Pacific Orange to test signal/ noise ratio of Pacific Orange stained peripheral blood cells in following standard emission filters 525/50, 585/42 and compared them to newly designed emission filters 550/20, 550/30, 550/40 by Omega Optical, Inc.

Summary

For the BD LSR2 instrument we found the 550/40 filter to be the best compromise solution. It eliminates a negative impact of spillover from Pacific Blue and minimizes the spillover from FITC and PE, while still improving the signal/background ratio over the default 525/50 filter. For the Dako Cyan ADP instrument we evaluated more limited number of samples and did not find any improvement over the 530/40 filter used by default.


45

ApPendix Lasers

Detectors

Helium-neon 200nm

300

400

Argon/Krypton

543 500

346 351

200nm

300

400

594

633

600

700

457 476 488 514

647 676

500

600

10

0

10

-2

700 10

-1

Argon Ion

346 351

200nm

350nm

457 476 488 514

300

400

500

600

450

550

650

yellow Dye Scotopic Eye Response Magenta Dye Photopic Eye Response Cyan Dye

Relative spectral response of the commonly used detectors: photopic human eye, scotopic human eye and color film.

700

750

Arc Lamps 100 10 0

Mercury

313 334 365

405 436

546

577 10 50

-2

10 0

-1

200nm

300

400

500

600

700

350nm

450

550

650

Bi-alkali PMT S20 PMT Extended Red PMT CCD Si Photodiode

Normalized response of bi-alkali PMT detector, extended red PMT detector, silicon detector, S20 PMT and CCD.

Xenon

750

LED 400

500

600

700

800

900

1000

precisExcite LED options

1100 fluorophore

200nm 300

mCherry Texas Red

CFP

Cy3.5 YFP

Cy3

Cy5

TRITC mRFP

400

500

600

700

800

900

LAMs

375

400

400

244-3403

425

450

475

445

500

490

244-3406

244-3413

465

244-3402

46

Alexa594

Alexa488

Normalised

Metal Halide

300nm

GFP FITC Pacific Blue

spectrum

Exfo™

DAPI Hoechst

505

244-3405

525

550

525

244-3401

535

244-3410


575

565

244-3411

600

595

244-3407

585

244-3412

625

650

635

244-3408

675

General Information Filter Types and Technology QuantaMAX: Omega Optical’s QuantaMAX filters are manufactured using state-of-the-art Energetic Process coating technology for high durability, and low temperature sensitivity. These high throughput filters provide excellent results in all applications. They are especially effective in applications where photons are scarce and exposure times are critical. QuantaMAX filter designs consider not just peak filter transmission and fluorophore probability curves, but also optimized band placement in relation to light sources, and blocking in relation to detector sensitivity. ALPHA Technology: Omega Optical’s proprietary coating process produces extremely steep slopes and precise edge location of cut-on and cut-off wavelengths. In addition, it provides the ability to place transmission and rejection regions exceptionally close together, and high attenuation between the passband and the rejection band. 3rd Millennium Technology: The most critical feature of filter design is the transition from blocking to transmission. Precise location of band edges, coupled with steep slopes, allows for optimal transmission of spectral energy. 3rd Millennium filters are specified by the critical cut-on and cut-off edges, resulting in much more accurate band placement and bandwidth, compared to industry standard filters that are defined by a Center Wavelength (CWL) and a Bandwidth (FWHM). Multi-band Filters: Omega Optical is a co-holder of the patent on all multi-band filters; including dual-band, tripleband, and quad-band designs. Others: Bandpass Filters Edge Filters Dichroic Mirrors Beamsplitters

Full Mirrors Neutral Density Filters Rejection Band Filters Color Correction Filters

Anti-Reflective Coatings Longpass Filters Shortpass Filters Hot/Cold Mirrors

Wideband Filters Multi-band Filters Narrow-band Filters

Custom Filters – OEM Production Quantities For optimum instrument system performance we recommend collaborative engineering for custom filter solutions. Omega Optical is a leading supplier for fluorescence based instruments and applications including: Microplate Readers, DNA Sequencers & Analyzers; Gene Chip Readers; Flow Cytometers and Cell Sorters; Real Time PCR Analyzers; Scanners and Imaging Systems; High-Throughput and High-Content Systems; Fluorescence Microscopes, and others.

Custom Filters – Small Quantities Custom filters in single or small quantities are available to ship in 5 – 10 days from our extensive inventory of components. Visit Build-a-Filter at www.omegafilters.com

Aftermarket filters If you are in need of a filter that did not come with your original equipment, please go to our Build-A-Filter tool on our website. Select your instrument, tell us your filter wave length, or cut-on or cut-off, and we do the rest. No need to specify physical dimensions. See page 47.

4
