Fluorescencelifetime molecular imaging can ... - Wiley Online Library

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Dec 18, 2013 - Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MA, USA. Key words. Fluorescence ... hemorrhagic ascites, molecular imaging, ovarian cancer ... Funding information.
Fluorescence-lifetime molecular imaging can detect invisible peritoneal ovarian tumors in bloody ascites Takahito Nakajima, Kohei Sano, Kazuhide Sato, Rira Watanabe, Toshiko Harada, Hirofumi Hanaoka, Peter L. Choyke and Hisataka Kobayashi Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MA, USA

Key words Fluorescence lifetime, fluorescence-guided surgery, hemorrhagic ascites, molecular imaging, ovarian cancer Correspondence Hisataka Kobayashi, Molecular Imaging Program, NCI/NIH, Building 10, Room B3B69, MSC 1088, Bethesda, Maryland 20892-1088, USA. Tel: +1-301-435-4086; Fax: +1-301-402-3191; E-mail: [email protected] Funding information Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research Received November 18, 2013; Revised December 17, 2013; Accepted December 18, 2013 Cancer Sci 105 (2014) 308–314 doi: 10.1111/cas.12343

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Blood contamination, such as bloody ascites or hemorrhages during surgery, is a potential hazard for clinical application of fluorescence imaging. In order to overcome this problem, we investigate if fluorescence-lifetime imaging helps to overcome this problem. Samples were prepared at concentrations ranging 0.3–2.4 lM and mixed with 0–10% of blood. Fluorescence intensities and lifetimes of samples were measured using a time-domain fluorescence imager. Ovarian cancer SHIN3 cells overexpressing the D-galactose receptor were injected into the peritoneal cavity 2.5 weeks before the experiments. Galactosyl serum albumin-rhodamine green (GSA-RhodG), which bound to the D-galactose receptor and was internalized thereafter, was administered intraperitoneally to peritoneal ovarian cancerbearing mice with various degrees of bloody ascites. In vitro study showed a linear correlation between fluorescence intensity and probe concentration (r2 > 0.99), whereas the fluorescence lifetime was consistent (range, 3.33  0.15– 3.75  0.04 ns). By adding 10% of blood to samples, fluorescence intensities decreased to