Jul 11, 2014 - stearate, zinc stearate, boric acid, polyethylene glycol and sodium stearyl fumarate. 8. The biopesticide formulation of claim 2, wherein the said ...
7/11/14
Patent WO2009093261A2 - Formulation of entomopathogenic fungus for use as a biopesticide - Google Patents +AKBAR
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Formulation of entomopathogenic fungus for use as a biopesticide WO 2009093261 A2 ABSTRACT The present invention relates to a biopesticide based on or more entomopathogenic fungi which is formulated as a novel highly compressed tablet and combinations thereof. The formulation exhibits longer shelf life and lesser contamination by saprophytic fungi. It also relates to a process of preparing the biopesticide formulation and modes of application of the same.
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Publication number Publication type Application number Publication date Filing date Priority date
WO2009093261 A2 Application PCT/IN2008/000620 Jul 30, 2009 Sep 26, 2008 Jan 24, 2008
Also published as
WO2009093257A2, WO2009093257A3, WO2009093261A3
Inventors
Divi Satyasayee, Reddy Parine Narsimha, Pathan Akbar Ali Khan, Uma Devi Koduru
Applicant
Divi Satyasayee
Export Citation
BiBTeX, EndNote, RefMan
Patent Citations (3), Classifications (2), Legal Events (2)
DESCRIPTION (OCR text may contain errors) FORMULATION OF ENTOMOPATHOGENIC FUNGUS FOR USE AS A BIOPESTICIDE
External Links: Patentscope, Espacenet
CLAIMS (OCR text may contain errors) Claims
Field of Invention What is claimed is: The present invention relates to a biopesticide based on one or more entomopathogenic fungi, which is formulated as a novel dispersible tablet resulting in longer shelf life preventing contamination with saprophytic fungi and facilitating easy application. It also relates to the process of preparing the formulation and use of it in combination with other pesticides. Background
1. A biopesticide formulation comprising conidia of one or more entomopathogenic fungi and other excipients in the form of a compressed tablet. 2. A biopesticide formulation comprising conidia of one or more entomopathogenic fungi, UV protectant, anti-saprophytic agent, dessicant, lubricant, binding agent, disintegrant and diluent.
The development of a good formulation is important for the successful utilization of commercial biopesticides. Factors affecting commercial formulations include
3. The biopesticide formulation of claim 1 , wherein the said
shorter shelf life, biological and physical properties of the formulation. It is
entomopathogenic fungi is selected from a group consisting of genera
desirable that the strength of the formulation is maintained over the two main
Beauveria, Metarhizium, Paecilomyces, Verticillium and Nomuraea.
cropping seasons and ideally even longer and hence a formulation with a longer shelf life is valuable for the manufacturers and end users alike. Currently available biopesticide formulations in the market are in the form of
4. The biopesticide formulation of claim 2, wherein the said UV protectant is selected from a group consisting of Tinopal, Blankophor BBH and Leucophor AP.
powder, granules and oil which have significant disadvantages namely high level
5. The biopesticide formulation of claim 2, wherein the said anti-saprophytic
contamination by saprophytic fungi and very low viability of the microbes leading
agent is chitin.
to low efficiency. The powder form further attracts moisture leading to contamination and subsequent loss of viability of the active ingredient (conidia) of
6. The biopesticide formulation of claim 2, wherein the said dessicant is
the biopesticide and the oil form suffers from poor spraying characteristics.
dicalcium phosphate.
Moreover, spraying of powder formulations leaves a lot of residual talc (the carrier used in these formulations) on the plant surfaces. Therefore, the available formulations though based on a very effective biocontrol agent have not gained popularity among farmers. These drawbacks should not be allowed to remain as an obstacle for the widespread use of these very promising biopesticides
7. The biopesticide formulation of claim 2, wherein the said lubricant is selected from a group consisting of talc, magnesium stearate, calcium stearate, zinc stearate, boric acid, polyethylene glycol and sodium stearyl fumarate.
(microbes) in biological control. The present invention aims to develop an
8. The biopesticide formulation of claim 2, wherein the said binding agent is
improved biopesticidal formulation. The said formulation is an entomopathogenic
selected from a group consisting of microcrystalline cellulose, cellulose
fungal-based biopesticide formulation, which shows considerably longer shelf life,
acetate, carrageenan, dextrin, glucose, ethyl cellulose and
minimized contamination and absorbance of moisture during storage and
polyvinylpyrrolidone.
handling. The tabletted nature of the biopesticide formulation which is safely packed (contamination resistant packing ex: strip, bottle etc) is first of its kind in
9. The biopesticide formulation of claim 2, wherein the said disintegrant is
fungal based biopesticides. The quick water dispersible nature of the formulation
selected from a group consisting of primogel, sodium starch glycolate,
with no or negligible contamination, long shelf life and components that ensure its
crospovidone and croscarmellose sodium.
effectivity in field conditions, all make it a valuable biopesticide addition handy in integrative crop insect management programmes. Summary
10. The biopesticide formulation of claim 2, wherein the said diluent is water. 11. A biopesticide formulation comprising by weight of about 20% of conidia of Beauveria bassiana, about 0.5 to 2% Tinopal LPW UNPA GX, about 2% to
The present invention relates to a biopesticide formulation comprising conidia of
10% of chitin, about 4% to 6% Primogel, about 50% to 60% dicalcium
one or more entomopathogenic fungi and other excipients in the form of a
phosphate, in the range of 2% to 5% magnesium sterate, about 10% to 16%
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7/11/14
Patent WO2009093261A2 - Formulation of entomopathogenic fungus for use as a biopesticide - Google Patents
compressed tablet. It also relates to the process of preparing the formulation and use of it in combination with other pesticides. In one aspect, the invention provides a biopesticide formulation comprising of conidia of one or more entomopathogenic fungi, UV protectant, anti saprophytic agent, dessicant, lubricant, binding agent, disintegrant and diluent. In another aspect, the invention provides a biopesticide formulation comprising by weight of about 20% conidia oϊBeauveria bassiana, about 0.5 to 2% Tinopal LPW UNPA GX, about 2 to 10% of chitin, about 4% to 6% Primogel, about 50% to 60% dicalcium phosphate, about 2 to 5% magnesium sterate, about 10% to 16% micro crystalline cellulose and 3% to 8% residual water content. The invention also provides application of the biopesticide formulation in combination with other pesticides selected from the group consisting of microbial, chemical and botanical insecticides. In yet another aspect, the invention provides a highly compressed tablet formulation of biopesticide packaged in moisture resistant strips or bottles to minimize the chance of contamination. The invention also provides a formulation
micro crystalline cellulose and 3% to 8% residual water content. 12. The biopesticide formulation of claim 1, wherein the said compressed tablet is packaged in moisture resistant strips or bottles. 13. A biopesticide formulation of claim 1 , wherein the said tablet is applied in combination with one or more pesticides selected from a group consisting of microbial, chemical and botanical insecticides. 14. The biopesticide formulation of claim 1, wherein the shelf life of the said formulation is at least one year at a temperature ranging from 00C to 38°C. 15. The biopesticide formulation of claim 1, wherein the shelf life of the said formulation is at least one year at a temperature of about 25 ± 2°C. 16. A method of killing an insect comprising administering the biopesticide formulation of claim 1 dispersed in water and sprayed on plants or dispensed directly in irrigation water or stagnant water. 17. A process of preparing the formulation of claim 1 comprising
with a shelf life of at least one year at a temperature ranging from O0C to 380C,
(a) mass culturing one or more entomopathogenic fungi through biphasic
more preferably at a temperature of about 25 ± 20C.
solid state fermentation to obtain a dry powder of conidia containing at least 1012 CFU of conidia per gram;
In yet another aspect, the invention provides the process of preparation of the biopesticide formulation. The processs comprises the steps of:
(b) geometrical mixing of the conidia with binders, lubricants, diluents, disintegrating agents, UV protectants, dessicants and anti saprophytic
(a) mass culturing one or more entomopathogenic fungi through biphasic solid
agents;
state fermentation to obtain a dry powder of conidia containing at least 1012 CFU conidia per gram;
(c) reducing the water content of the mixture up to
