Gene expression during preimplantation stage - Development - The ...

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1737

Development 127, 1737-1749 (2000) Printed in Great Britain © The Company of Biologists Limited 2000 DEV3150

Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse development Minoru S. H. Ko1,2,*, John R. Kitchen1, Xiaohong Wang1, Tracy A. Threat1, Xueqian Wang1, Aki Hasegawa3, Tong Sun1, Marija J. Grahovac1,2, George J. Kargul1,2, Meng K. Lim1,2, YuShun Cui1, Yuri Sano2, Tetsuya Tanaka2, Yuling Liang1, Scott Mason1, Paul D. Paonessa1, Althea D. Sauls1, Grace E. DePalma1, Rana Sharara1, Lucy B. Rowe4, Janan Eppig4, Chris Morrell5 and Hirofumi Doi3,6,* 1ERATO Doi Bioasymmetry Project, JST, Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI 48202, USA 2Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging, NIH, Baltimore, MD 21224-6820, USA 3ERATO Doi Bioasymmetry Project, JST, WBG Marive East 12F, 2-6 Nakase, Mihama-ku, Chiba 261-7112, Japan 4The Jackson Laboratory, Bar Harbor, Maine 04609, USA 5Statistics and Experimental Design Section, Research Resources Branch, National Institute on Aging, NIH, Baltimore, MD 21224-6820, USA 6Fujitsu Labs Ltd, Mihama-ku, Chiba 261-0023, Japan

*Authors for correspondence (e-mail: [email protected] or [email protected])

Accepted 28 January; published on WWW 21 March 2000

SUMMARY Little is known about gene action in the preimplantation events that initiate mammalian development. Based on cDNA collections made from each stage from egg to blastocyst, 25438 3′-ESTs were derived, and represent 9718 genes, half of them novel. Thus, a considerable fraction of mammalian genes is dedicated to embryonic expression. This study reveals profound changes in gene expression that include the transient induction of transcripts at each

stage. These results raise the possibility that development is driven by the action of a series of stage-specific expressed genes. The new genes, 798 of them placed on the mouse genetic map, provide entry points for analyses of human and mouse developmental disorders.

INTRODUCTION

stage of development mainly on maternally stored mRNAs and proteins to drive the regional differentiation of embryonic cells (Wieschaus, 1996). But mammals significantly modify this program. Some oocyte mRNAs are translated, but fertilization triggers massive mRNA degradation (Piko and Clegg, 1982). Two major events then occur. One is the transcriptional activation of the zygotic genome. The timing of this transition is regulated by a ‘zygotic clock’ (Nothias et al., 1995; Schultz, 1993), and is somewhat species-dependent (at the late one-cell stage in mouse, 4- to 8-cell in human and 8- to 16-cell stage in sheep; Schultz, 1993). The second major event, compaction, occurs at the 8- to 16-cell stage, when cells that were previously loosely associated begin to adhere in the tightly organized cell mass of the morula. This is the starting point for cell differentiation into Inner Cell Mass (ICM), which eventually becomes the embryo, and Trophectoderm, which eventually becomes the placenta. By the 32- to 64-cell stage (blastocyst), the two cell types are clearly distinguishable. Now released from the zona pellucida, the blastocyst is implanted in the uterus. A primary obstacle that has delayed molecular analysis of this developmental program is the difficulty of collecting and

Preimplantation development of mammalian embryos is marked by many critical and unique events, including the start of zygotic transcription, the first cell differentiation and the initiation of specific cell-cell adhesion (reviewed in Hogan et al., 1994; Pedersen, 1986; Rossant, 1986; Watson et al., 1992). Analysis of these processes is fundamental for the understanding of organ formation, for practical techniques such as veterinary cloning of animals (Wakayama et al., 1998; Wilmut et al., 1997), and for clinical applications such as in vitro fertilization (IVF) and the assessment of fetal well-being. In spite of its importance, very little is known about the molecular events during this early phase of development. At a macro level, the outline of the process has become clear for several organisms. During preimplantation development, the embryo, confined within the zona pellucida, does not change in overall size. Rather, an increase in cell number is compensated by a decrease in cell size, giving rise to the terminology of ‘cleavage stages’. In most species studied, including Drosophila, C. elegans, Xenopus, sea urchins and fish, morphological changes and cell differentiation rely at this

Key words: Mouse development, Preimplantation, cDNA analysis, EST, Stage-specific gene, Gene mapping

1738 M. S. H. Ko and others analyzing large numbers of eggs and embryos. In early studies, expression patterns of a limited number of genes observed by several methods led to the idea that, notwithstanding the dynamic changes, gene expression is monotonous: once gene expression has begun, it is not switched off, and the encoded proteins then accumulate as development proceeds to the blastocyst stage (reviewed in Kidder, 1992; Schultz and Heyner, 1992; Watson et al., 1992). Later analyses with highresolution two-dimensional protein gels revealed dynamic changes in quantities of many proteins during the 1- to 4-cell stages (Latham et al., 1991) and the 8-cell to blastocyst stages (Shi et al., 1994), but only a limited number of genes have been identified so far (Schultz, 1999). Similarly some attempts to construct cDNA libraries (Adjaye et al., 1997, 1998; Rothstein et al., 1992, 1993; Sasaki et al., 1998; Taylor and Piko, 1987) and examine gene expression patterns by mRNA differential display (Oh et al., 1999; Schultz, 1999) have provided only short bits of transcripts and fragmentary information. Aiming at a global survey of gene expression and a definition of the number of genes that are preimplantationspecific, we have adapted techniques to generate cDNA libraries from each stage of preimplantation mouse embryos, carried out large-scale sequencing of cDNAs from each stage, and mapped 798 of the novel species on the mouse genome. The results support the inferences that (1) a significant fraction of the genome is dedicated to genes expressed specifically in early development, adding considerably to the nascent catalogue of mammalian genes; (2) genes coexpressed in the same stage tend to cluster in the genome; and (3) the expressed genes include cohorts acting in a stage-specific manner that may suggest a ‘hit and run cascade’ model for the developmental process.

MATERIALS AND METHODS Mouse preimplantation embryo collection Eggs and embryos were collected by standard methods (Hogan et al., 1994). C57BL/6J female mice were superovulated and mated with C57BL/6J male mice. Unfertilized eggs were collected without mating. Embryos from all the other stages were collected by killing the pregnant mice at 0.5, 1.5, 2.5 and 3.5-days post coitum (d.p.c.). Embryos were staged by visual inspection under the stereomicroscope. To avoid undesirable effects of culturing the preimplantation embryos, all the embryos up to the blastocyst stages were collected by flushing the oviduct and uterus. Construction of stage-specific cDNA libraries The seven cDNA libraries were constructed from each of seven stages of preimplantation development in essentially the same manner as previously described (Takahashi and Ko, 1994). The normalization and mechanical shortening of cDNA inserts steps were omitted. In brief, total RNAs were extracted from 1528 unfertilized eggs and double-stranded cDNA was synthesized by a kit (Life Technology, Superscriptase II) with an oligo(dT)NotI primer (5′-pGACTAGTTCTAGATCGCGAGCGGCCGCCC15(T)-3′) from 2.7 µg of total RNA. The double-stranded cDNAs were treated with T4 DNA polymerase and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal3 (LL-Sal3A: 5′-pGCTATTGACGTCGACTATCC-3′, LL-Sal3B: 5′-pGGATAGTCGACGTCAAT-3′). The cDNAs were purified by phenol/chloroform and separated from free linkers by Centricon 100. Then, cDNAs were amplified by long-range high-fidelity PCR using Ex Taq polymerase (Takara) for 25 cycles

under the following conditions: denature at 94°C for 20 seconds, 25 cycles of 94°C for 10 seconds, 68°C for 10 minutes (plus 20 seconds for each additional cycle), and a final extension at 72°C for 10 minutes, on a Perkin-Elmer GeneAmp PCR system 9600. Then, the cDNAs were purified by phenol/chloroform and by Centricon 100. The cDNAs were double-digested with SalI and NotI enzymes. Next, the cDNAs were purified by phenol/chloroform extraction and ethanol-precipitated. Then, the cDNAs were size-selected by Size Fractionation Column (Life Technology, Fraction 8 to 10). The cDNAs were ethanol-precipitated and cloned into the SalI/NotI site of pSPORT1 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by chemical methods. The other libraries were constructed essentially in the same manner. For the fertilized egg library, double-stranded cDNA was synthesized from 5.4 µg of total RNA extracted from 1137 fertilized eggs. For the 2-cell library, double-stranded cDNA was synthesized from 1.2 µg of total RNA extracted from 397 embryos. For the 4-cell library, doublestranded cDNA was synthesized from 2.6 µg of total RNA extracted from 32 embryos. For the 8-cell library, double-stranded cDNA was synthesized from 4.3 µg of total RNA extracted from 230 embryos. For the 16-cell library, double-stranded cDNA was synthesized with an oligo(dT)GC primer 5′-pGACTAGTTCTAGATCGCGAGCGGCCGCGC15(T)-3′ from 2.1 µg of total RNA extracted from 42 embryos. For the blastocyst library, double-stranded cDNA was synthesized with an Oligo(dT)-1 primer 5′-GAGAGAGACTAGTTCTAGATCGCGAGCGGCCGC18(T)-3′ from 1.5 µg of total RNA extracted from 40 embryos. A single-path sequencing of cDNA clones A single-path cDNA sequencing was conducted as described (Ko et al., 1998). The 96-well microtiter plates were thawed and cDNA clones were inoculated into a 1 ml deep-well 96-microtiter plate (Beckman). Plasmid preparations from the cDNA clones were performed with Qiagen’s 96-well format REAL-prep system. The plasmid DNAs were resuspended in 50 µl TE (8.0) buffer. 5 µl of DNA were used for cycle-sequencing reactions. The first 2000 Blastocyst ESTs were sequenced using standard dye primer chemistry (Perkin-Elmer-ABI). The ESTs from all other libraries, and the remaining 4000 Blastocyst ESTs, were sequenced using ET-dye primer chemistry (Amersham). All sequencing reactions were performed by an ABI Prism 877 Integrated Thermal Cycler (PerkinElmer-ABI). Sequence data analyses Clustering of 3′-EST sequences was done using the Blast2 program (Altschul et al., 1990). The criteria for identifying the unique gene set will be described elsewhere (A. H. and H. D., in preparation). In brief, all the 3′-ESTs were searched against each other for sequence similarities. For each EST, hits were sorted according to the score and the difference of scores between that EST and each of the hits were examined. Hits with a score greater than 70% of the highest score (generated by an EST’s homology to itself) in each list were classified to the same group. All the ESTs below this threshold were classified to other gene sets. Estimation of gene expression levels by EST frequency The EST data sets from each cDNA library were subjected to Blast2 analyses against the set of 9718 unique genes. Then, the frequency of EST appearance for each gene was tabulated. The 95% confidence interval for each EST frequency in a total 3000 EST set is as follows: 0 EST matches, sample proportion 0, (0, 0.0012); 1 EST matches, sample proportion 0.0003 (0, 0.0018); 2 EST matches, sample proportion 0.007, (0.0001, 0.0023); 9 EST matches, sample proportion 0.0030, (0.0013, 0.0055). Therefore, differences among 0 matches, 1 match and 2 matches are not statistically significant. According to Fisher’s exact test results, 7 EST matches in one library are required to have a statistically significant difference from 1 EST

Gene expression during preimplantation stage 1739 match in another library (1-sided; P=0.035). Similar results were obtained by the formula that has been developed to test the significance of differential gene expression in EST/SAGE projects (Audic and Claverie, 1997; Claverie, 1999). Application of this formula to a total 3000 EST set in each cDNA library indicates a differential gene expression with a probability greater than 0.96 and less than 0.97 in the following combinations of EST matches: 5 EST matches in one library and 0 EST matches in another library, 7 EST matches in one library and 1 EST match in another library, 9 EST matches in one library and 2 EST matches in another library, 11 EST matches in one library and 3 EST matches in another library, 13 EST matches in one library and 4 EST matches in another library, and so on.

on a customized polyacrylamide gel electrophoresis system using 10% non-denaturing polyacrylamide gels. Electrophoresis was performed for 1 hour at 250 volts. The gels were stained with ethidium bromide and photographs were taken on a UV transilluminator. Only the PCR primer pairs that exhibited heteroduplex bands were used in the gene mapping study. Approximately 1000 primer pairs fell into this category. Assembly of the PCR reactions was performed with the Biomek1000 robotic workstation (Beckman). Genotyping of The Jackson Laboratory BSS Interspecific Backcross DNAs (94 N2 animals plus C57BL/6JEi and SPRET/Ei parental DNAs) was scored by visual inspection and analyzed by the Map Manager computer program (Manly, 1993).

RT-PCR analyses For each stage, 10 embryos were collected under the microscope and stored in 10 µl BGJb Medium (Life Technology). Embryos were collected and directly lysed in 0.05% NP40. Samples were sequentially diluted in fivefold steps and subjected to RT-PCR. Reverse transcription and PCR amplification were performed using EzrTth RNA PCR kit (Perkin-Elmer) in 50 µl reaction mixtures containing 25 mM manganese acetate, 0.2 units rTth DNA polymerase, 10 ng/µl primers (for the Alpha03732 gene: 5′-GTTCCAGGAGACTAAGTTTCCGTG3′, 5′-AGGCTGTCCATCAGAAAGTTGCT-3′; for the gamma-actin gene: 5′-TTCCTGCGCAGATCGCAA-3′, 5′-GTGACAATGCCGTGTTCGATAGG-3′), 10 mM dNTPs, 250 mM Bicine (pH 8.2), 575 mM potassium acetate and 10 µl RNAs. Reactions were incubated at 60°C for 30 minutes for reverse transcription, 94°C for 1 minute for preheating, 40 cycles of PCR at 94°C for 15 seconds and 58°C for 30 seconds, followed by the final extension at 58°C for 7 minutes. The PCR products were electrophoresed on a 3% agarose gel and the gel was stained with SYBR Green. The gel was analyzed with a STORM phosphor Imager (Molecular Dynamics).

Data and cDNA clones access All cDNA clones reported in this paper are available from the American Type Culture Collection (ATCC: http://www.atcc.org/) or RIKEN DNA Bank (http://www.rtc.riken.go.jp/DNA/HTML/ engsearch.html and http://www.rtc.riken.go.jp/DNA/mouse_info.html). cDNA sequence information is available through Entrez and BLAST servers at NCBI,

Genetic mapping of new ESTs New ESTs were mapped on the mouse genetic map by using The Jackson Laboratory BSS Interspecific Backcross Panel (Rowe et al., 1994). PCR primer pairs were developed from approximately 350 bp of the most 3′-end of the cDNA sequences to increase the chance of having sequence polymorphisms between C57BL/6J and M. Spretus (Takahashi and Ko, 1993). Primers were designed as a batch in a semiautomatic manner on a Sun Workstation, UNIX platform. The Unix version of PRIMER program developed by the WI/MIT Mouse Genome Center (http://www-genome.wi.mit.edu) was used as a core engine of our primer design program. The front end and the back end of the programs were written by our group. A total of 4500 primer pairs were developed during the course of this work. The primer pairs are available from the Research Genetics (http://www.resgen.com/). To test for sequence polymorphisms, genomic DNAs of C57BL/6J, M. spretus, and an equimolar mixture of C57BL/6J and M. spretus, were amplified by the each primer pair. The PCR products were run

Number of 3′′-ESTs

Fig. 1. Stages of preimplantation development and number of collected 3′-ESTs.

Table 1. Summary of ESTs Embryonic stage Cluster number Classified (Alpha clusters) ESTs matched to named gene ESTs matched to other libraries ESTs not matched to other libraries ESTs not matched to named gene ESTs matched to other libraries ESTs not matched to other libraries Unclassified (Beta clusters) Others (Gamma clusters) Total

9 718 955 888 67 8 763 4 412 4 351 28 2 9 748

Unfertilized Fertilized egg egg 2 823 266 253 13 2 557 1 458 1 099 273 0 3 096

2 983 473 450 23 2 510 1 837 673 331 0 3 314

2-cell

4-cell

8-cell

16-cell

Blastocyst

Total

3 068 432 418 14 2 636 1 824 812 619 0 3 687

2 793 496 485 11 2 297 1 740 557 217 1 3 011

3 194 579 556 23 2 615 2 060 555 249 0 3 443

2 945 536 517 19 2 409 1 918 491 250 0 3 195

5 349 994 978 16 4 355 3 012 1 343 340 3 5 692

23 155 3 776 3 657 119 19 379 13849 5 530 2 279 4 25 438

1740 M. S. H. Ko and others Table 2. Examples of classification of ESTs based on sequence similarity Embryonic stage Unique gene ID

Sequence ID

Alpha03578 Alpha01399 Alpha01806 Alpha06817 Alpha06441 Alpha02151 Alpha03119 Alpha06127 Alpha01784 Alpha01880 Alpha04955 Alpha06934 Alpha01097

gb|AF000982|HSAF000982 gb|U05341|RRU05341 gb|AF016099|AF016099 gb|M18252|MUSIAPL31 gb|U02599|MMU02599 gb|U17088|MMU17088 gb|U94402|MMU94402 gb|U72059|MMU72059 gb|S63728|S63728 emb|Z19581|MMSIAH2A emb|X57413|MMTGFB2 gb|L43326|MUSGC1R gb|U58512|MMU58512

Alpha03973 Alpha00486 Alpha00110 Alpha02232 Alpha02292 Alpha03168 Alpha04477 Alpha05510 Alpha06107 Alpha06532 Alpha01370 Alpha00724 Alpha03605 Alpha02058 Alpha00280 Alpha01214 Alpha03336 Alpha01217 Alpha03842 Alpha04360 Alpha00707 Alpha03821 Alpha03514 Alpha02997 Alpha06386 Alpha06401 Alpha03511 Alpha03762 Alpha00937 Alpha01106 Alpha01861 Alpha01916 Alpha04172 Alpha01332 Alpha01954 Alpha01522 Alpha00165 Alpha01375 Alpha03477

gb|S78271|S78271 gb|M22995|HUMKREV1A dbj|D50264|D50264 gb|U20159|MMU20159 emb|Y15740|MMSV15740 gb|J04022|RATATPSRA gb|U95116|MMU95116 emb|X60672|MMRAD gb|M80631|MUSGNA14A emb|Y08460|MMMDES gb|U53456|MMU53456 emb|X13986|MMPONTIN gb|U27323|MMU27323 gb|U67187|MMU67187 gb|AE000664|MMAE000664 gb|M58567|MUSHSD3B gb|U58883|MMU58883 emb|X62940|MMTSC22 gb|U57343|MMU57343 dbj|D86728|D86728 gb|M14044|MUSCALP gb|M96823|MUSNUCLEOB gb|J03750|MUSBPP9 gb|U49350|MMU49350 emb|X04017|MMSPARCR gb|U96726|MMU96726 gb|U37720|MMU37720 gb|U75361|RNU75361 emb|X51438|MMVIM gb|M21065|MUSIRF1B gb|U39302|MMU39302 emb|X67644|MMGLY96 gb|AF004107|AF004107 emb|X51829|MMMDPRMR gb|S72537|S72537 gb|U16818|MMU16818 dbj|D45860|MUSMDPPB4B dbj|D16262|MUS121A gb|U51907|MMU51907

Alpha00933 Alpha01224 Alpha01260 Alpha02846 Alpha03366 Alpha04823

gb|U32745|U32745 gb|J04596|MUSSPKC gb|M12253|CRUTUBAB gb|M64085|MUSSPI2A gb|S43105|S43105 gb|M84361|RATCSF1A

Alpha01484 Alpha03369 Alpha05298 Alpha00608 Alpha01107 Alpha01532 Alpha01874 Alpha02056 Alpha03281 Alpha03347 Alpha03577

gb|U67187|MMU67187 emb|Z71173|MMIP3R2 gb|U67874|MMU67874 gb|L76155|MUSMHBAT4R emb|X55957|MMINAS gb|U87557|MMU87557 emb|X71327|MMMTF1 emb|X78682|MMBAP32 gb|U01063|MMU01063 gb|U13838|MMU13838 gb|U38690|MMU38690

Alpha04706 Alpha04707 Alpha04749 Alpha05109 Alpha05121 Alpha06366 Alpha06891 Alpha06987 Alpha00574 Alpha02751 Alpha02003 Alpha02539 Alpha04709 Alpha01875 Alpha02801 Alpha00830

dbj|D16333|MUSCPP gb|U36757|MMTHREC02 gb|AF001688|AF001688 gb|AC000399|AC000399 dbj|D38517|MUSDHM1P gb|M29324|MUSL1A1 gb|U48972|MMU48972 gb|U04672|MMU04672 emb|X64070|MMCDMPR emb|X14607|MM24P3 gb|AC000398|AC000398 emb|X57413|MMTGFB2 emb|V00711|MITOMM dbj|D14077|MUSSGP2 emb|X64837|MMOATMR gb|M20495|MUSPROL

Description Homo sapiens dead box, X isoform (DBX) mRNA, alternative transcript 2 Rattus norvegicus p55CDC mRNA, complete cds Mouse L1 repetitive element; Mus musculus glycine receptor beta-subunit gene, partial cds Mouse retrovirus-like intracistronic type A particle element DNA, clone L31 Mouse A12 mRNA; Mus musculus Balb/c xlr3a mRNA, complete cds Mus musculus MT transposon-like element clone MTi6 Mus musculus ubiquitin conjugating enzyme UBC9 mRNA, complete cds Mus musculus chloride channel regulator Icln (Icln) pseudogene JAK1 protein=protein tyrosine kinase [mice, eye, mRNA, 4191 nt] M. musculus siah-2 protein mRNA Mouse mRNA for transforming growth factor-beta2 Mus musculus coiled-coil protein (CG-1) mRNA, complete cds Mus musculus Rho-associated, coiled-coil forming protein kinase p160 ROCK-1 mRNA, complete cds SB1.8/DXS423E=mitosis-specific chromosome segregation protein SMC1 homolog Human ras-related protein (Krev-1) mRNA, complete cds Mouse mRNA for phosphatidylinositol glycan class F, complete cds Mus musculus 76 kDa tyrosine phosphoprotein SLP-76 mRNA, complete cds Moloney murine sarcoma virus mRNA for mos gene Rat brain Ca+2-ATPase mRNA, complete cds Mus musculus lissencephaly-1 protein (LIS-1) mRNA, complete cds M. musculus mRNA for radixin Mouse G protein alpha subunit (GNA-14) mRNA, complete cds Mus musculus mRNA for Mdes transmembrane protein Mus musculus protein phosphatase 1cgamma (PP1cgamma) mRNA; Mouse mRNA for PP1gamma Mouse mRNA for minopontin; Murine gene for osteopontin Mus musculus Cdc25a (cdc25a) mRNA, complete cds Mus musculus G protein signaling regulator RGS2 (rgs2) mRNA, complete cds Mus musculus TCR beta locus; Mouse virus-like (VL30) retro-element Mus musculus delta-5-3-beta-hydroxysteroid dehydrogenase/delta-5-> delta-4 isomerase (Hsd3b) Mus musculus c-Cbl associated protein CAP mRNA, complete cds M. musculus TSC-22 mRNA Mus musculus homeobox protein Meis2 mRNA, complete cds Mouse mRNA for topoisomerase-inhibitor suppressed, complete cds Mouse mRNA for protein-tyrosine kinase substrate p36 (calpactin I heavy chain), complete cds Mouse nucleobindin mRNA, complete cds Mouse single stranded DNA binding protein p9 mRNA, complete cds Mus musculus CTP synthetase mRNA, complete cds Mouse mRNA for cysteine-rich glycoprotein SPARC; Mouse p2-4 mRNA for SPARC/osteonectin Mus musculus vibrator critical region, phosphatidylinositol transfer protein alpha (Pitpn) Mus musculus CDC42 mRNA, complete cds Rattus norvegicus Munc13-3 mRNA, complete cds Mouse mRNA for vimentin Mouse interferon regulatory factor 1 mRNA, complete cds Mus musculus 26S proteasome subunit 4 ATPase mRNA, complete cds M. musculus gly96 mRNA Mus musculus unknown protein mRNA, complete cds Mouse myeloid differentiation primary response mRNA encoding MyD116 protein zebrin II [mice, C57BL/6J inbred, P20 cerebella, mRNA, 1587 nt] Mus musculus UDP glucuronosyltransferase (UGT1-06) mRNA, complete cds Mouse mRNA for magnesium dependent protein phosphatase (protein phosphatase 2C) beta-4 Mouse mRNA encoding unknown protein, complete cds Mus musculus TRAF family member associated NF-kappa B activator (TANK) mRNA, complete cds Haemophilus influenzae Rd section 60 of 163; E. coli rrnH gene for rRNAs and tRNAs Mouse platelet-derived growth factor-inducible KC protein mRNA, complete cds Chinese hamster alpha-tubulin II mRNA; Mouse alpha-tubulin isotype M-alpha-6 mRNA Mouse spi2 proteinase inhibitor (spi2/eb1) mRNA, 3′ end Cycb1=cyclin B1 [mice, mRNA, 2387 nt] Mouse macrophage colony-stimulating factor (4 kb) mRNA; Rat CSF-1 protein mRNA, complete cds Mus musculus G protein signaling regulator RGS2 (rgs2) mRNA, complete cds M. musculus mRNA for inositol 1,4,5-trisphosphate receptor (type 2) Mus musculus fat facets homolog (Fam) mRNA, complete cds Mus musculus Bat-4 gene, complete cds M. musculus mRNA for inhibin alpha subunit Mus musculus phosphatidylcholine-specific phospholipase D2 (mPLD2) mRNA, complete cds M. musculus mRNA for MRE-binding transcription factor M. musculus mRNA for B-cell receptor associated protein (BAP) 32 Mus musculus pLK serine/threonine kinase mRNA; Mus musculus protein kinase (Plk) mRNA Mus musculus vacuolar adenosine triphosphatase subunit B gene, complete cds Mus musculus DAZ-like putative RNA binding protein mRNA; Homo sapiens dead box, X isoform (DBX) Mouse mRNA for coproporphyrinogen oxidase, complete cds Mus musculus thrombin receptor (Cf2r) gene, exon 2 and complete cds Mus musculus U4/U6 snRNP 90 kDa protein gene, complete cds Genomic sequence from Mouse 9, complete sequence (Mus musculus) Mouse mRNA for Dhm1 protein, complete cds Mouse L1Md-A13 repetitive sequence Mus musculus spindlin (Spin) mRNA, complete cds Mus musculus type I receptor BRK-1 mRNA, complete cds M. musculus gene for cation-dependent mannose-6-phosphate receptor Mouse SV-40 induced 24p3 mRNA Genomic sequence from Mouse 11, complete sequence (Mus musculus) Mouse mRNA for transforming growth factor-beta2 Mouse mitochondrial genome Mouse mRNA for sulfated glycoprotein-2; Mus musculus alpha-clustrin and beta-clustrin mRNA M. musculus Oat mRNA for ornithine aminotransferase Mouse cathepsin L gene; Mouse mRNA for major excreted protein (MEP); Mouse cysteine proteinase

U

F

2

4

8

16

B

5 3 2 2 2 2 3 2 2 2 2 2 4

1 1 0 0 1 1 0 0 1 0 0 0 1

0 0 0 0 0 1 0 1 0 1 1 1 0

1 0 0 0 0 0 0 0 0 0 0 0 0

0 1 0 0 0 0 0 1 1 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 1 0 0 0 1 0 0 0 0 0 0

5 4 3 3 2 2 2 2 2 2 2 3 2 3 3 2 2 3 2 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0

0 0 0 0 0 0 0 0 0 0 0 9 2 3 6 5 3 5 4 2 5 2 3 2 2 2 4 2 4 3 3 6 2 4 2 3 2 2 2

0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 4 2 0 0 0 0 0 0 0 1 1 0 0 0 1 1 0 0 1 1 1 1

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 1 1 1 1 0 0 0 0

0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1 1 1 1 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0

3 3 3 3 3 3

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

0 0 0 0 0 0

1 1 1 0 0 0 0 0 0 0 0

2 2 2 2 2 2 2 2 2 2 2

0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0

2 2 2 2 2 2 2 2 2 2 2 3 3 2 2 3

0 0 0 0 0 0 0 0 3 4 2 2 3 3 2 7

0 0 0 0 0 0 0 0 1 1 1 0 2 6 3 5

0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 6

0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2

0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1

1 1 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Gene expression during preimplantation stage 1741 Table 2. Continued Embryonic stage Unique gene ID Alpha00363 Alpha00210 Alpha00483 Alpha03072 Alpha01538 Alpha04396 Alpha05193 Alpha05193 Alpha01066 Alpha01085 Alpha05436 Alpha02606 Alpha00697 Alpha01730 Alpha03529 Alpha03719 Alpha02659 Alpha03475 Alpha03879 Alpha05210 Alpha01388 Alpha01653 Alpha03727 Alpha04019 Alpha04841 Alpha00121 Alpha00488 Alpha01229 Alpha01281 Alpha02538 Alpha03157 Alpha03263 Alpha04346 Alpha04915 Alpha06067 Alpha06638 Alpha07088 Alpha07324 Alpha00014 Alpha01337 Alpha02878 Alpha01980 Alpha00237 Alpha00237 Alpha00503 Alpha00973 Alpha01389 Alpha00130 Alpha00345 Alpha01052 Alpha00594 Alpha02766 Alpha00839 Alpha05164 Alpha06789 Alpha02706 Alpha05168 Alpha04165 Alpha00438 Alpha01109 Alpha05063 Alpha05758 Alpha01215 Alpha01929 Alpha03573 Alpha02521 Alpha03020 Alpha02095 Alpha00819 Alpha01922 Alpha02006 Alpha03253 Alpha00220 Alpha00259 Alpha02018 Alpha07894 Alpha03403 Alpha00592

Sequence ID

Description

emb|X06406|MML40KD gb|M88335|MUSTUMSEQA gb|U52822|MMU52822 dbj|D88315|D88315 emb|X13605|MMH33REP gb|U94593|MMU94593 gb|U45977|MMU45977 dbj|D50461|D50461 emb|X90875|MMFXR1PRT gb|S71186|S71186 gb|L25255|MUSRANBP1 emb|AJ223794|MMU223794 gb|AF003346|AF003346

Mouse mRNA for translational controlled 40 kDa polyyeptide p40; Mouse laminin receptor mRNA M. musculus mRNA sequence; R. norvegicus putative v-fos transformation effector protein (Fte-1) Mus musculus ornithine decarboxylase antizyme mRNA, complete cds Mouse mRNA for tetracycline transporter-like protein, complete cds Murine mRNA for replacement variant histone H3.3 Mus musculus uncoupling protein homolog (UCPH) mRNA; Mus musculus UCP2 mRNA Mus musculus calcium-binding protein Cab45a mRNA, complete cds Mouse SDF4 mRNA, complete cds M. musculus mRNA for FXR1 protein XPBC/ERCC-3=DNA repair gene [mice, mRNA, 2673 nt] Mus musculus Ran/TC4 binding protein (RanBP1) mRNA Mus musculus CDC10 gene, exon 13 Mus musculus ubiquitin-conjugating enzyme UbcM2 mRNA; Homo sapiens Xp22 BAC GSHB-257G1 dbj|D17571|MUSCYPOR Mouse mRNA for NADPH-cytochrome P450 oxidoreductase, complete cds dbj|D89063|D89063 Mus musculus mRNA for oligosaccharyltransferase, complete cds gb|U62483|MMU62483 Mus musculus ubiquitin conjugating enzyme (ubc4) mRNA, complete cds dbj|D01034|MUSTFIID Mus musculus mRNA for TFIID; Mus musculus domesticus transcription factor IID (Tbp) mRNA gb|S43105|S43105 Cycb1=cyclin B1 [mice, mRNA, 2387 nt] gb|U08440|MMU08440 Mus musculus Balb/c cytochrome c oxidase subunit VIaL mRNA, complete cds gb|U28016|MMU28016 Mus musculus parathion hydrolase (phosphotriesterase)-related protein mRNA, complete cds gb|S59342|S59342 nuclear pore complex glycoprotein p62 [mice, mRNA, 2411 nt] gb|U89506|MMU89506 Mus musculus Mlark mRNA, complete cds gb|U47737|MMU47737 Mus musculus thymic shared antigen-1 (TSA-1) gene; Mus musculus C57BL/6 Sca-2 precursor gb|S82156|S82156 GST-5=glutathione S-transferase-sperm antigen MSAg-5 fusion protein {3′ region} [mice, testis] gb|M35797|MUSTCP1X Mouse t-complex protein (Tcp-1x) mRNA, 3′ end gb|L16846|MUSBTG1X Mouse BTG1 mRNA, complete cds gb|U31758|MMU31758 Mus musculus transcriptional regulator RPD3 homolog mRNA, complete cds gb|U13262|MMU13262 Mus musculus myelin gene expression factor (MEF-2) mRNA, partial cds gb|U58105|MMU58105 Mus musculus Btk locus, alpha-D-galactosidase A (Ags) and Bruton’s tyrosine kinase (Btk) genes gb|U10871|MMU10871 Mus musculus MAP kinase mRNA; Mouse mRNA for p38b gb|L36244|MUSMAT Mus musculus metalloproteinase matrilysin mRNA, complete cds gb|M60474|MUSMARCKS Mouse myristoylated alanine-rich C-kinase substrate (MARCKS) mRNA, complete cds gb|AC003996|AC003996 Mouse Cosmid ma66a097 from 14D1-D2 (T-Cell Receptor Alpha Locus), complete sequence emb|V00711|MITOMM Mouse mitochondrial genome emb|X91144|MMRNAPSGL M. musculus mRNA for P-selectin glycoprotein ligand 1 gb|U57692|MMU57692 Mus musculus N-terminal asparagine amidohydrolase (Ntan1) mRNA, complete cds gb|U13371|MMU13371 Mus musculus clone 1.5 novel mRNA from renin-expressing kidney tumor cell line gb|L11651|RATEIF5 Rattus norvegicus eukaryotic initiation factor 5 (eIF-5) mRNA, complete cds emb|X79233|MMEWS M. musculus EWS mRNA gb|AF011644|AF011644 Mus musculus oral tumor suppressor homolog (Doc-1) mRNA; M.musculus mRNA poly(A) site sequence gb|J03298|MUSULT Mouse uterine lactotransferrin mRNA emb|X81987|MMTAX107 M. musculus mRNA for TAX responsive element binding protein 107 gb|M73436|MUSRSP4 Mouse ribosomal protein S4 (Rps4) mRNA, complete cds emb|X14210|RNRPS4 Rat mRNA for ribosomal protein S4 gb|L04280|MUSRPL12A Mus musculus ribosomal protein (Rpl12) mRNA; Rat mRNA for ribosomal protein L12 emb|X52803|MMCYCM Mouse mRNA for cyclophilin (EC 5.2.1.8); Rat housekeeping protein P31 mRNA emb|X82636|RNUQL40 R. norvegicus mRNA for a fusion protein of ubiquitin and ribosomal protein L40 emb|X68282|RNRPL13A R. norvegicus mRNA for ribosomal protein L13a; Rattus norvegicus hexokinase type III mRNA dbj|D55720|MUSNPTCC Mouse mRNA for nuclear pore-targeting complex; Mus musculus pendulin (pendulin) mRNA dbj|D17653|MUSHBL2B Mouse mRNA for HBp15/L22, complete cds emb|X61433|MMSODPOT M. musculus mRNA for sodium/potassium ATPase beta subunit gb|J03941|MUSFERH Mouse ferritin heavy chain (MFH) mRNA, complete cds emb|Z31553|MMCCTBE M. musculus (129/Sv) Cctb mRNA for CCT (chaperonin containing TCP-1) beta subunit emb|X99395|MMENOGD M. musculus gene encoding endonuclease G gb|L12383|RATADPRF4A Rattus norvegicus ADP-ribosylation factor 4 mRNA, complete cds emb|X02487|MMMURS Mouse retrovirus-related DNA sequence (MuRRS); Mouse middle repetitive LTR-like DNA sequence emb|Z14249|MMERK1 M. musculus mRNA for mitogen activated protein kinase (erk-1) emb|X74856|MMRNAL28 M.musculus L28 mRNA for ribosomal protein L28 gb|U43206|MMU43206 Mus musculus phosphatidylethanolamine binding protein mRNA, complete cds gb|U49351|MMU49351 Mus musculus lysosomal alpha-glucosidase mRNA, complete cds gb|L10652|RATEIF2B Rattus rattus eukaryotic initiation factor (Eif-2) 67 kDa associated protein mRNA, complete cds emb|X60831|MMUBF Mouse ubf gene for transcription factor UBF dbj|D17614|D17614 Rattus norvegicus mRNA for 14-3-3 protein theta-subtype, complete cds gb|AF030343|AF030343 Mus musculus peroxisomal/mitochondrial dienoyl-CoA isomerase ECH1p (Ech1) mRNA gb|M64301|RATERK3 Rat extracellular signal-related kinase (ERK3) mRNA, complete cds gb|M29462|MUSMDHA Mouse malate dehydrogenase mRNA, complete cds gb|U70210|MMU70210 Mus musculus TR2L mRNA, partial cds emb|X52046|MMCOL3A1 M. musculus COL3A1 gene for collagen alpha-I; R. norvegicus mRNA for pro alpha 1 collagen type III gb|S80082|S80082 Murine leukemia virus gag protein; gag...env {provirus} gb|M63245|MUSALASH Mus musculus amino levulinate synthase (ALAS-H) mRNA, 3' end gb|S45012|S44957S7 Tapa-1=integral membrane protein TAPA-1; M. musculus MD3 mRNA gb|U05809|MMU05809 Mus musculus LAF1 transketolase mRNA, complete cds gb|M12660|MUSH Mouse CFh locus, complement protein H gene; Mouse factor H mRNA gb|J02622|MUSASPATM Mouse mitochondrial aspartate aminotransferase isoenzyme mRNA, complete cds gb|M27938|MUSMEA Mouse male-enhanced antigen mRNA (Mea), complete cds gb|U83896|RNU83896 Rattus norvegicus sec7B mRNA, complete cds gb|U47328|MMU47328 Mus musculus MHC class I heavy chain precursor (H-2K(b)) mRNA; Mus musculus PYS-2 mRNA gb|U27457|MMU27457 Mus musculus origin recognition complex protein 2 homolog mORC2L mRNA, complete cds

U, unfertilized egg; F, fertilized egg; 2, 4, 8 and 16, 2-, 4-, 8- and 16-cell embryos; B, blastocyst; cds, coding sequence.

U

F

2

4

8

16

B

0 0 1 0 1 0 0 0 0 0 0 0 0

2 6 5 0 1 0 0 0 0 0 0 0 0

5 3 2 2 3 3 2 2 3 3 2 2 3

4 5 3 0 1 1 1 1 0 0 0 0 1

7 5 3 1 0 0 0 0 0 0 0 1 0

2 5 4 0 0 0 0 0 0 1 1 0 0

19 14 3 1 1 1 1 1 1 0 0 0 0

0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 1 0 0 0 0 1 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1

2 2 5 3 3 3 3 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 3

1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0

0 1 1 1 0 1 0 1 1 1 1 0 0 0 0 0

3 2 4 4 2 2 4 3 3 1 0 1 1 0 0 1

2 7 6 6 4 5 2 2 2 2 5 4 4 2 2 6

0 10 5 5 2 4 5 2 2 0 0 0 0 0 0 0

0 4 13 13 6 4 4 8 6 1 0 0 0 0 0 1

0 17 10 10 7 6 5 3 3 1 1 1 1 1 1 0

0 0 1 0 0 0 0 0 0 0 0 0

1 0 1 0 0 0 0 0 0 1 0 0

0 0 0 0 0 0 0 0 0 0 0 1

2 2 4 3 3 2 7 5 5 4 4 3

0 0 1 1 1 1 0 0 0 0 0 0

1 1 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 1 0 0 0 1 0

0 0 1 1 1 1 1 1 0 0

0 0 1 1 0 0 0 0 0 0

3 3 2 2 2 2 2 2 2 2

0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0

1742 M. S. H. Ko and others NIH (http://www.ncbi.nlm.nih.gov/). Detailed map locations of genes are accessible through The Jackson Laboratory Backcross DNA Mapping Resource [http://www.jax.org/resources/documents/cmdata/ bkmap/BSS.html]. Information about gene clustering and expression profile is available at ERATO Doi Project Home page (http://www.bioa.jst.go.jp/pge/). PCR primer pairs are available from the Research Genetics (http://www.resgen.com/). Finally the detailed information about cDNA clones, sequences, PCR primer pairs, and the library-specific BLAST search is available at the Laboratory of Genetics home page (http://lgsun.grc.nia.nih.gov).

Group A: Low expession U F

No. No. No. 2 4 8 M B Known Unknown Total % Total 499 6766 7265 74.76%

Group B: Constitutive expession U F

No. 2 4 8 M B Known

Group C: Complex expession U F 2 4 8M B

Global changes of gene expression patterns during preimplantation development For each unique gene, the number of reads from each cDNA library was summed. Table 2 shows an example of this summation, using ‘named genes’; complete results are available through the World Wide Web http://www.bioa.jst.go.jp/pge/. Since the frequency of ESTs in a particular cDNA library corresponds roughly to the expression level of the gene (Okubo et al., 1992), the data compiled here provide a first approximation of gene expression levels at each stage. To assess changes in gene expression, the 9718 unique gene set was grouped into four main patterns based on the EST frequency at each stage (Fig. 2). Since one EST in

No. 143

RESULTS Construction of cDNA libraries and characterization of ESTs cDNA libraries were constructed from each of seven mouse preimplantation stages (Fig. 1). The cDNAs were directionally cloned, with an average size of insert of about 1.5 kb. cDNA clones from each library were arrayed in 96-well microtiter plates and about 400 bp sequenced from 3′ termini. All 25,438 Expressed Sequence Tags (ESTs) obtained were deposited in the public sequence database and have been made available to the scientific community since the summer of 1997 [GenBank accession numbers: C75935-C81630; C85044-C88357, AU014577-AU024803, AU040095-AU046300]. By comparing the EST sequences to the repetitive sequence database, 2279 ESTs containing repeat sequences were identified (Beta clusters in Table 1). ESTs with low complexity sequence information were also identified and excluded from the further analyses (Gamma clusters in Table 1). The rest of the ESTs (23,155, Alpha clusters in Table 1) were condensed to a set of 9718 unique genes based on sequence similarity searches against one another. Similar genes were sought by BlastN search of the non-redundant (nr) public sequence database. Only 10% of the genes (955) showed close matches and were identified as known [named] genes (e.g. Table 2; for more complete information, refer to http://www.bioa.jst.go.jp/pge/). Furthermore, when similar ESTs were sought by the BlastN program against NCBI’s public EST database (dbEST; Boguski et al., 1993), only 55% (5300) showed close matches to at least one EST from human, mouse, or rat. Considering the large number of ESTs (Adams et al., 1991; Hillier et al., 1996; Hwang et al., 1997; Marra et al., 1999, 1998; Okubo et al., 1992) in the dbEST (>1×106 for human, >4×105 for mouse, and >2×105 for rat), the rate of discovery of new ESTs in these cDNA libraries is very high. It supports the notion that many genes expressed in mammalian preimplantation stages have not been otherwise isolated.

No. No. Unknown Total % Total 1 10 11 0.11%

173

316

3.25%

Group D: Single-peak expession U F 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

No. 2 4 8 M B Known 23 7 2 0 45 4 3 1 3 35 3 0 0 8 42 7 0 1 6 59 7 7 49 12 110

No. No. Unknown Total % Total 268 291 2.99% 24 31 0.32% 9 11 0.11% 2 2 0.02% 198 243 2.50% 8 12 0.12% 1 4 0.04% 1 2 0.02% 4 7 0.07% 247 282 2.90% 8 11 0.11% 1 1 0.01% 1 1 0.01% 1 9 0.09% 206 248 2.55% 6 13 0.13% 3 3 0.03% 2 3 0.03% 0 6 0.06% 288 347 3.57% 18 25 0.26% 7 14 0.14% 338 387 3.98% 0 12 0.12% 51 161 1.66%

Fig. 2. Grouping of genes based on the global expression patterns. U, unfertilized; F, fertilized; 2, 4, 8, 2-cell, 4-cell and 8-cell embryos; M, morula; B, blastocyst.

approximately 3000 obtained from each developmental stage may be present by chance particularly for genes with very low level of expression, the initial analyses have focused mainly on genes with relatively abundant expression, i.e. genes represented by more than two independent clones in a cDNA library. Though this criterion is still statistically weak for individual genes (see Materials and Methods section), the groupings provide an indication of global changes. The majority of genes (75%) were in Group A ‘Low expression’ throughout preimplantation development. A very small fraction (0.11%) of genes showed constitutive expression throughout preimplantation development (Group B). Some genes (3.25%) showed complex expression patterns (Group C) that may reflect up-and-down regulation but are probably also affected by sampling statistics. The rest of the genes (22%) were classified in ‘Single-peak expression’ (Group D). This group consists of genes undergoing: (1) gradual degradation from maternally stored mRNAs (3.44%, Groups D1-D4) and (2) constitutive expression once the gene is activated at a certain stage (2.14%, Groups D9, D14, D19, D22, D24 and D25). But it also includes an unexpectedly large number of genes (17.2%) that show apparently stage-specific expression.

Gene expression during preimplantation stage 1743 Fig. 3. RT-PCR analyses of selected genes. For each stage, total RNAs extracted from ten embryos were sequentially diluted in fivefold steps (×25, ×125 and ×625) and subjected to RT-PCR. (A) Alpha03732 gene selected as an example of a 2-cell stage-specific expressed gene from Table 2. (B) Cytoplasmic gamma-actin gene selected as an example of a constitutively expressed gene from Table 2.

A

B

Table 3. Examples of stage-specific expressed genes Gene ID Alpha00603 Alpha02663 Alpha01916 Alpha00797 Alpha00096 Alpha01107 Alpha03732 Alpha01766 Alpha01215 Alpha04688 Alpha01866 Alpha01929 Alpha03573 Alpha01262 Alpha04643 Alpha01192 Alpha06657 Alpha01500 Alpha00739 Alpha01031 Alpha01563 Alpha00199 Alpha00425 Alpha00754 Alpha01891 Alpha03190 Alpha02045 Alpha02117 Alpha02259 Alpha03351 Alpha01216 Alpha01188 Alpha01005 Alpha00537 Alpha00913 Alpha01176 Alpha00893 Alpha00075 Alpha04585 Alpha01597 Alpha00195 Alpha00285

Gene Name

Unfertilized Fertilized Egg Egg (3096) (3314)

Unknown, but similar to Human E2 ubiquitin conjugating enzyme UbcH5C mRNA Unknown, but similar to autoantigen [human, thyroid associated ophthalmopathy] Mouse gly96 mRNA Unknown Unknown Mouse inhibin alpha-subunit exon 2 Unknown Unknown Rat mRNA for 14-3-3- protein theta-subtype protein kinase regulator Unknown, but similar to Human insulin-like growth factor binding protein-4 (IGFBP4) Mouse alpha-1 protease inhitor 5 (alpha-1 PI-5) Mouse peroxisomal/mitochondrial dienoyl-CoA Rat extracellular signal-related kinase (ERK3) mRNA Unknown Mouse gene for sphingomyelin Phosphodiesterase Unknown Unknown Unknown Unknown Mouse mRNA for 2,3-bisphosphoglycerate mutase Unknown Unknown Unknown Unknown Mouse S-adenosyl homocysteine hydrolase Unknown, but similar to bone morphogenetic protein type 1A receptor Mouse zinc finger protein Requiem (req) mRNA Unknown Unknown Unknown M. musculus mRNA for radixin Human ring zinc-finger protein (ZNF127-Xp) gene Mouse mRNA for heparin binding protein-44 Mouse DNA for t-haplotype-specific elements Unknown, but simiar to Mouse gene for topoisomerase I Rat mRNA for CIC-K1 protein M. musculus mRNA for connexin31 Mouse cytoplasmic gamma-actin Mouse mRNA for elongation factor 1-alpha (EF 1-alpha) Mouse mRNA for G protein beta subunit homologue Mouse nucleolar protein N038 mRNA Mus musculus ATP synthase beta-subunit (beta-F1 ATPase)

The total number of ESTs at each embryonic stage are given in parentheses.

2-cell Embryo (3684)

4-cell Embryo (3011)

8-cell Embryo (3444)

16-cell Embryo Blastocyst (3195) (5692)

13

3

5

0

0

0

0

7

1

0

0

0

0

0

0 0 0 0 0 0 0

6 4 5 2 0 0 0

1 0 0 0 7 0 0

1 0 0 0 0 8 7

0 0 0 0 0 0 0

0 0 1 0 0 0 0

0 0 0 0 0 0 0

0

0

0

6

0

0

0

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

6 5 5 5 0 0 0 0 0 0 0 0 0 0 0 0

2 0 0 0 8 6 6 5 2 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 9 7 7 6 6 6 6 6

0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0

0 0 0 0 0 0 0 0 0 0 0 3 4 3 3 2

0 0 0 0 2 0 0 0 0 0 0 5 9 2 1 1

0 0 0 0 0 0 0 0 0 0 0 1 8 3 3 5

0 0 0 0 0 0 2 0 0 0 0 4 25 5 3 3

0 0 0 0 0 1 0 0 0 0 0 4 30 5 6 2

5 5 5 5 3 0 0 0 0 0 0 4 28 6 3 13

0 0 0 0 0 15 14 11 6 6 3 14 71 15 3 10

Chromosome 2

Chromosome 3

D2Ertd168e D2Ertd544e

D1Ertd164e D1Ertd701e

Chromosome 4

D2Wsu23e D1Ertd705e

D6Ertd109e D6Ertd352e D6Ertd439e D6Ertd588e D6Wsu176e

D5Ertd543e

D1Ertd228e

D3Ertd789e

D2Ertd217e

D1Ertd448e D1Wsu40e

Lhx3 D2Ertd97e D2Ertd434e D2Wsu81e D2Wsu129e

D1Ertd799e

D1Ertd84e

D4Ertd389e

D3Ertd740e

D4Ertd510e D4Ertd582e

D3Ertd246e

D2Ertd44e D2Ertd369e D2Wsu17e D2Wsu34e D2Wsu141e

D1Ertd259e

D4Ertd360e

D3Ertd254e

D3Ertd751e

D3Ertd491e D3Wsu174e D3Ertd270e

D2Ertd694e D2Ertd112e D2Ertd145e D2Ertd198e D2Ertd295e D2Ertd612e

D4Ertd58e D4Wsu36e

D1Ertd161e D1Ertd408e D2Ertd120e D2Ertd329e

D2Ertd391e D2Ertd640e D2Ertd706e D2Wsu101e

D1Ertd757e D1Ertd816e D1Wsu66e

D4Ertd279e D4Wsu125e

D3Wsu133e

D4Ertd89e D4Ertd335e D4Ertd346e

D3Ertd207e D3Wsu79e D3Ertd508e D3Ertd598e D3Ertd731e D3Ertd775e Bglap1 D3Ertd299e

D2Ertd468e D1Ertd564e

D3Ertd194e

D2Ertd282e

D1Ertd62e D1Ertd101e D1Ertd399e D1Ertd622e

D2Ertd127e D2Ertd520e

D1Ertd507e

D2Ertd337e

D1Ertd57e D1Ertd347e D1Wsu22e D1Ertd5e D1Ertd273e D1Ertd202e D1Ertd148e D1Ertd182e D1Ertd218e D1Ertd308e D1Ertd367e D1Ertd811e D1Ertd819e D1Wsu198e D1Ertd185e D1Ertd147e D1Ertd475e D1Ertd532e D1Ertd646e D1Ertd251e D1Ertd471e D1Ertd754e

D2Ertd504e D2Wsu127e

D3Ertd229e D3Wsu161e

D2Ertd52e D2Ertd473e D2Ertd634e D2Wsu131e

D3Ertd343e

D1Wsu1e

D3Ertd211e D3Ertd425e D3Ertd250e D3Ertd302e D3Ertd330e D3Ertd258e

D4Ertd13e D4Ertd196e D4Ertd442e D4Wsu53e D4Ertd264e D4Ertd478e D4Ertd76e

76.6

D2Ertd63e D2Ertd93e D2Ertd239e

D2Wsu85e

87.2

D1Ertd396e D1Ertd654e

93.6

D7Ertd760e D7Ertd795e D7Wsu69e Cckbr D7Ertd684e Adm D7Ertd70e D7Ertd344e D7Ertd743e D7Ertd805e D7Wsu130e

D6Wsu120e

D7Ertd602e D7Ertd183e D7Ertd629e D7Wsu105e D7Wsu128e

D5Ertd406e D5Ertd460e D5Ertd470e D5Ertd679e D5Ertd159e

D6Ertd263e

D6Ertd385e* D6Ertd35e D6Ertd375e D6Ertd380e D6Wsu147e D6Wsu189e

D5Ertd724e Zp3 D5Ertd655e D5Wsu46e

D6Ertd131e D6Wsu163e

Actb D5Ertd525e D5Ertd559e D5Ertd591e D5Ertd605e D5Ertd683e D5Wsu45e

D6Ertd90e D6Ertd213e D6Wsu113e D6Ertd3e D6Ertd469e D6Ertd474e D6Ertd782e D6Ertd87e

D5Ertd440e D5Ertd505e

79.8

D4Ertd151e D4Ertd792e D4Ertd429e D4Ertd432e D4Ertd571e D4Wsu35e D4Wsu114e D4Wsu124e D4Ertd41e D4Ertd117e D4Wsu188e D4Ertd296e

Fig. 4. For legend see p. 1746.

D7Ertd304e D7Ertd373e D7Ertd388e D7Ertd481e D7Ertd487e D7Ertd495e D7Ertd523e D7Ertd807e D7Wsu86e

D6Ertd47e D6Ertd490e D6Wsu116e

D5Ertd355e

D4Ertd22e D4Wsu27e

D2Ertd173e D2Ertd342e D2Wsu143e

D7Ertd74e

D5Ertd189e D5Ertd255e D5Ertd444e D5Ertd708e D5Wsu150e

D4Ertd20e D4Ertd31e D4Ertd78e D4Ertd111e D4Ertd796e D4Ertd639e D4Ertd222e D4Ertd361e D4Ertd561e D4Ertd617e D4Ertd767e D4Wsu29e D4Ertd669e D4Ertd804e D4Wsu43e D4Ertd314e D4Ertd421e D4Wsu24e D4Wsu33e D4Wsu144e D4Ertd184e D4Ertd100e D4Ertd179e D4Ertd220e D4Ertd632e D4Ertd810e D4Wsu199e

D3Ertd162e D3Ertd568e

D7Ertd152e D7Ertd791e

D6Ertd71e D6Ertd318e D6Ertd349e D6Wsu16e D6Wsu137e

D5Ertd585e D5Ertd689e D5Ertd33e D5Ertd40e D5Ertd483e

D4Ertd274e D4Ertd290e D4Ertd298e D4Ertd765e

D3Ertd108e

D2Ertd186e

D7Ertd783e D7Ertd348e D7Ertd237e D7Ertd257e

D6Ertd32e D6Ertd245e D6Ertd527e D6Ertd538e

D5Ertd431e

D4Ertd618e D4Ertd513e D4Ertd681e

D2Ertd105e D2Ertd397e D2Ertd459e D2Ertd554e D2Ertd691e D2Wsu107e D2Wsu140e D2Ertd501e D2Ertd48e D2Ertd92e D2Ertd113e D2Ertd303e D2Ertd122e D2Ertd695e D2Wsu39e D2Ertd535e D2Wsu58e

D7Ertd715e D7Ertd758e

D5Ertd215e

Ifna8 D4Ertd27e D4Ertd103e D4Ertd321e D4Ertd786e

D3Ertd711e D3Wsu106e D3Wsu167e D3Ertd547e D3Wsu60e

D6Ertd253e D6Ertd286e D6Ertd384e D6Ertd456e D6Ertd746e D6Wsu157e*

D5Ertd66e D5Ertd650e D5Ertd774e D5Wsu111e D5Wsu145e

D3Ertd537e D3Ertd383e D3Ertd801e

Ltk D2Ertd210e D2Ertd435e D2Ertd485e D2Ertd616e D2Ertd750e

D5Ertd102e D5Wsu31e

D6Ertd157e

D3Ertd557e

D1Ertd291e D1Ertd576e

D6Ertd234e D6Ertd365e D6Ertd404e D6Ertd772e

D5Ertd287e D5Ertd577e D5Ertd593e D5Ertd606e

D3Ertd34e

D1Wsu84e

D5Ertd77e D5Ertd163e D5Ertd301e D5Ertd601e D5Ertd700e

D5Ertd236e D5Ertd566e D5Wsu152e

D4Wsu132e

D3Ertd176e D3Ertd300e

D2Ertd794e

Crygb

D7Ertd462e D7Wsu180e D7Ertd565e Cox7a1 D7Ertd146e D7Ertd353e D7Ertd764e D7Ertd1e D7Ertd128e D7Ertd316e D7Ertd351e D7Ertd784e D7Ertd312e D7Ertd685e Klk1 D7Ertd649e D7Ertd671e D7Ertd156e Ldh3 D7Ertd413e D7Ertd526e

D5Ertd135e D5Ertd227e D5Ertd249e

D4Ertd628e

D2Ertd624e

Cd152 D1Ertd8e D1Ertd53e D1Ertd692e D1Wsu158e

D7Ertd59e D7Ertd143e D7Ertd177e D7Ertd445e D7Ertd458e D7Ertd486e D7Ertd595e D7Ertd661e D7Wsu1e D7Wsu21e D7Wsu62e D7Wsu190e

Tcrb D6Ertd160e D6Ertd631e D6Wsu1e

D5Ertd371e D5Ertd615e D5Ertd798e

D4Ertd174e D4Ertd199e D4Ertd659e

Chromosome 7

D6Ertd14e D6Ertd15e D6Ertd313e D6Ertd415e

D5Ertd260e D5Ertd422e D5Ertd477e D5Wsu178e D5Ertd110e D5Ertd521e D5Ertd579e D5Wsu110e D5Wsu148e D5Wsu185e

D3Ertd265e D3Ertd797e

D2Wsu32e

D1Ertd83e D1Ertd702e

D5Ertd121e D5Ertd149e D5Ertd363e

D3Ertd452e

D2Ertd357e D2Wsu88e D2Ertd542e D2Ertd742e

D1Ertd230e D1Ertd309e D1Ertd762e

96.8

D5Ertd560e

D3Ertd555e

D1Ertd433e

D1Ertd704e D1Ertd10e D1Ertd86e D1Ertd578e D1Ertd75e

Chromosome 6

D4Ertd800e D4Wsu139e

Il7 D3Ertd138e D3Ertd552e

D2Ertd623e

Chromosome 5

76.6

Expression Levels 0.2% ≤ x < 1% 0.04% ≤ x < 0.2% 0.008% ≤ x < 0.04% 0% ≤ x < 0.008%

70.2

D7Ertd558e D7Ertd45e D7Ertd443e D7Ertd677e D7Wsu41e D7Wsu87e D7Ertd753e D7Ertd517e D7Ertd680e Cyp2e1 D7Ertd187e H19 Ins2 D7Ertd193e D7Wsu30e D7Wsu37e

1744 M. S. H. Ko and others

Chromosome 1

68.1

Acta1 D8Ertd233e D8Ertd325e D8Ertd580e D8Ertd590e D8Ertd620e D8Ertd713e D8Wsu26e

D8Ertd56e D8Ertd106e D8Ertd370e D8Ertd377e

D8Ertd503e

D8Ertd362e D8Ertd515e D8Ertd633e D8Wsu151e D8Ertd54e D8Ertd698e D8Wsu108e D8Ertd107e

D8Ertd158e D8Ertd374e D8Ertd587e

D8Ertd28e D8Ertd281e D8Ertd323e D8Wsu61e

D8Ertd575e

D8Ertd69e D8Ertd563e D8Ertd572e D8Ertd294e D8Ertd738e D8Ertd812e D8Wsu49e

D8Wsu96e D8Wsu38e D8Ertd769e

D8Ertd268e D8Ertd514e D8Ertd594e D8Ertd124e D8Ertd790e D8Ertd252e D8Ertd381e D8Ertd814e D8Ertd21e D8Ertd674e

D8Ertd531e

D8Ertd82e D8Ertd91e D8Ertd238e D8Ertd766e

D8Ertd2e D8Ertd317e D8Ertd354e D8Ertd419e

D8Ertd67e D8Ertd130e D8Ertd350e D8Ertd457e D8Ertd569e D8Ertd51e D8Ertd319e

D8Ertd387e

D8Ertd118e

Chromosome 8

67.0

D9Ertd192e D9Ertd382e D9Ertd402e D9Ertd662e D9Ertd788e D9Wsu172e

D9Ertd25e

D9Ertd428e

D9Ertd660e

D9Ertd256e D9Ertd393e D9Ertd241e

Dag1 D9Ertd292e D9Ertd418e D9Wsu10e

D9Ertd280e D9Ertd356e

D9Ertd267e D9Ertd305e D9Ertd809e D9Ertd306e*

D9Ertd167e D9Ertd411e D9Ertd464e D9Ertd815e D9Ertd338e D9Wsu18e D9Wsu191e D9Ertd392e D9Ertd414e D9Ertd423e D9Wsu138e Cox7a3 D9Ertd133e D9Wsu20e D9Wsu90e D9Wsu168e

D9Ertd26e D9Ertd85e D9Ertd278e D9Ertd341e D9Ertd394e D9Ertd496e D9Wsu1e D9Wsu74e D9Wsu149e

D9Ertd334e

D9Ertd596e

D9Ertd12e D9Ertd60e D9Ertd818e

D9Ertd115e D9Ertd285e D9Ertd720e

Chromosome 9

63.8

D10Ertd625e D10Ertd739e D10Ertd718e D10Wsu1e D10Ertd43e D10Ertd68e D10Ertd284e D10Ertd709e D10Ertd773e D10Ertd516e D10Ertd447e D10Ertd584e D10Ertd664e D10Ertd73e D10Ertd610e D10Wsu93e Dagk1 Admr D10Ertd722e

D10Ertd126e

D10Ertd802e

D10Wsu52e

D10Wsu102e

D10Ertd438e D10Ertd140e D10Ertd494e D10Ertd533e D10Ertd641e D10Ertd17e D10Ertd645e D10Wsu42e Egr2 D10Ertd638e D10Ertd203e D10Ertd214e D10Ertd749e D10Ertd322e Adn D10Ertd116e D10Ertd190e D10Ertd345e D10Ertd378e D10Ertd567e D10Ertd761e

D10Wsu179e D10Ertd276e D10Ertd690e D10Ertd755e D10Wsu136e D10Wsu159e

D10Ertd667e

D10Ertd398e D10Wsu183e

D10Ertd733e

Chromosome 10

54.2

D12Ertd551e

D12Ertd549e D12Wsu95e

D12Ertd771e*

D12Ertd247e D12Ertd7e D12Ertd644e D12Ertd647e

D12Ertd123e D12Wsu1e D12Wsu118e D12Ertd748e D12Wsu28e

D12Ertd673e D12Ertd777e

D12Ertd216e D12Ertd364e D12Ertd522e

D12Ertd19e D12Ertd125e D12Ertd407e D12Ertd512e

Lamb1-1 D12Ertd446e

D12Ertd604e

D12Ertd129e

D12Ertd427e

D12Ertd137e D12Ertd208e D12Ertd553e

D12Ertd482e

Chromosome 12

Fig. 4. For legend see p. 1746.

65.9

D11Ertd729e Csfg Hoxb7 D11Ertd134e D11Ertd307e D11Ertd717e D11Ertd752e D11Ertd99e D11Ertd333e D11Ertd707e D11Ertd736e D11Wsu68e D11Ertd400e D11Ertd726e D11Ertd768e D11Ertd4e D11Ertd498e D11Ertd546e D11Ertd636e D11Wsu47e D11Wsu173e Fasn D11Ertd195e D11Ertd204e D11Ertd712e D11Ertd759e D11Wsu175e D11Wsu197e

D11Ertd379e D11Ertd395e D11Ertd437e D11Wsu48e D11Wsu78e

Nf1 D11Ertd9e D11Ertd18e D11Ertd72e D11Ertd80e D11Ertd530e

D11Ertd172e D11Ertd518e D11Wsu182e D11Ertd672e

D11Wsu80e D11Ertd686e D11Ertd714e

Csfgm D11Ertd142e D11Ertd175e D11Ertd416e D11Ertd461e D11Ertd497e D11Ertd539e

D11Ertd153e

D11Ertd730e D11Wsu99e

Gabra6 D11Ertd603e

D11Ertd506e

D11Ertd49e

D11Ertd326e D11Ertd540e D11Ertd676e

D11Ertd39e D11Ertd166e D11Ertd619e

Chromosome 11

76.6

D13Ertd275e

D13Ertd94e D13Ertd104e D13Ertd608e

D13Ertd699e

D13Wsu121e

D13Ertd776e

D13Ertd37e

D13Ertd688e

D13Ertd324e D13Ertd476e

D13Ertd332e

D13Ertd219e D13Ertd614e D13Wsu156e

D13Ertd570e D13Wsu50e D13Ertd261e D13Ertd328e

Ctla2a Tpbp D13Ertd205e D13Ertd311e D13Ertd297e D13Ertd666e D13Wsu123e

D13Ertd340e D13Ertd372e

D13Ertd376e D13Wsu177e

D13Ertd42e D13Ertd787e D13Ertd548e

D13Ertd150e D13Wsu115e

D13Wsu1e

D13Ertd224e D13Ertd656e Pl1 D13Wsu14e

D13Ertd212e

Plf

D13Wsu64e D13Ertd463e D13Ertd524e

Chromosome 13

58.5 cM

D14Ertd64e D14Wsu89e

D14Ertd16e D14Ertd728e*

D14Ertd24e

D14Ertd426e

D14Ertd581e

D14Ertd611e

D14Ertd310e D14Ertd453e

D14Ertd231e D14Ertd719e D14Ertd732e

D14Ertd484e D14Ertd668e D14Wsu146e

D14Ertd209e D14Ertd500e D14Wsu171e

D14Ertd114e D14Ertd436e

D14Ertd630e

D14Ertd226e

D14Ertd449e D14Ertd574e D14Ertd171e D14Ertd817e D14Ertd420e

Plau D14Ertd170e D14Ertd670e D14Ertd725e D14Ertd813e

Chromosome 14

Gene expression during preimplantation stage 1745

D15Ertd50e D15Ertd136e D15Ertd180e D15Ertd529e D15Ertd597e D15Wsu59e D15Wsu122e D15Ertd600e D15Ertd30e D15Ertd221e D15Ertd806e D15Ertd412e D15Ertd154e D15Ertd586e D15Wsu126e D15Ertd336e

Chromosome 16

Chromosome 17

D16Ertd465e D16Wsu65e D16Wsu194e D16Ertd95e D16Ertd727e D16Ertd780e D16Ertd803e D16Wsu103e D16Ertd6e D16Ertd36e D16Ertd266e D16Ertd607e D16Ertd778e D16Ertd779e D16Wsu119e

D16Ertd248e D16Ertd269e D15Ertd509e D15Ertd556e

D15Ertd417e D15Ertd424e D15Wsu75e D15Wsu192e D15Ertd244e D15Ertd181e D15Ertd320e D15Ertd781e D15Ertd55e D15Ertd466e D15Ertd785e D15Ertd492e

45.7

D15Ertd271e D15Ertd405e D15Ertd528e D15Ertd682e D15Ertd366e D15Ertd735e D15Ertd119e D15Ertd430e D15Ertd697e D15Wsu77e D15Wsu97e

D16Ertd480e D16Ertd642e D16Wsu73e

Chromosome 19

D18Ertd693e D18Ertd139e D18Ertd331e D18Ertd723e

Plg D17Ertd141e D17Ertd648e D17Ertd663e D17Wsu1e D17Wsu19e D17Wsu134e D17Wsu155e D17Ertd197e D17Ertd262e D17Ertd441e D17Ertd657e D17Wsu11e D17Wsu15e D17Wsu51e D17Wsu82e D17Wsu196e D17Ertd455e D17Wsu92e D17Ertd592e D17Ertd165e D17Ertd277e D17Ertd488e D17Ertd562e D17Ertd29e D17Ertd288e D17Ertd716e D17Wsu76e D17Wsu91e D17Ertd710e D17Ertd808e D17Wsu166e D17Wsu193e D17Ertd191e D17Ertd763e

D15Ertd489e D15Ertd621e

D15Ertd23e D15Ertd81e D15Ertd741e D15Ertd747e D15Wsu169e

Chromosome 18

Chromosome X

DXErtd697e DXErtd793e

D19Ertd98e D19Ertd144e D19Ertd283e D19Ertd609e D19Ertd627e D19Ertd703e D19Ertd721e D19Ertd678e D19Wsu55e

D18Ertd289e D18Ertd643e Ttr D18Ertd201e D18Ertd232e D18Ertd293e D18Ertd188e D18Ertd243e D18Ertd390e D18Ertd511e D18Ertd665e D18Ertd734e D18Wsu154e

DXErtd11e

DXErtd770e

Lpc1 D19Ertd756e D19Wsu54e D19Ertd79e D19Ertd744e

D18Ertd451e D18Ertd613e D18Wsu100e D19Ertd410e D19Wsu12e Lmnb1 D18Ertd65e D18Wsu181e

DXWsu72e

D19Wsu57e

D18Ertd169e

D17Wsu94e

D18Ertd155e

D16Ertd450e D17Ertd479e D17Ertd599e D17Wsu104e D17Wsu160e D17Ertd403e

D19Ertd200e

D18Ertd653e

D19Ertd675e D18Wsu70e D18Ertd240e D18Wsu187e

D16Wsu83e

D19Wsu44e

D18Ertd206e D18Wsu98e

D17Wsu164e

D19Ertd386e D19Ertd626e

D16Ertd454e

D19Ertd132e D19Ertd409e

D16Wsu109e D17Ertd178e D17Ertd315e

D16Ertd88e D16Ertd502e

41.5

DXErtd223e

D19Ertd652e D19Wsu162e D19Wsu195e Ins1

D16Ertd519e D16Ertd536e D16Ertd550e D17Ertd96e D17Ertd589e

DXErtd573e D19Ertd737e

D16Ertd472e D16Ertd534e

51.1

DXWsu67e

53.2

D16Ertd272e D16Ertd493e D16Ertd61e

60.6 cM

DXErtd242e

77.6

Fig. 4. Gene map with expression profiles. Expression levels of individual genes are shown in seven small boxes next to locus names and are color coded based on the frequency of ESTs in each cDNA library. The cDNA libraries represented by each box are from right to left: unfertilized egg, fertilized egg, 2-cell embryo, 4-cell embryo, 8-cell embryo, 16-cell embryo and blastocyst. Ertd markers in blue are newly mapped in this

paper. Wsu markers in red are derived from extraembryonic tissues of 7.5-d.p.c. embryos (Ko et al., 1998). Markers in black are some known genes we mapped previously (Ko et al., 1994). The total chromosome length is shown in cM at the bottom of each chromosome.

1746 M. S. H. Ko and others

Chromosome 15

Gene expression during preimplantation stage 1747 Stage-specific expressed genes Expression patterns with a sharp peak in only one stage were observed for each stage. For successive stages, fertilized eggspecific genes (Group D5) comprised 2.50% of the total RNAs; 2-cell-specific (Group D10), 2.90%; 4-cell-specific (Group D15), 2.55%; 8-cell-specific (Group D20), 3.57%; Morulaspecific (Group D23), 3.98%; and Blastocyst-specific (Group D25), 1.66%. Table 3 shows examples of such stage-specific expressed genes selected for a statistically significant level of expression that is comparable to the level for classical constitutively expressed genes like actin. Of course, the EST frequencies in each cDNA library only roughly correlate with the expression level of genes, and there are two possible artifacts that could make these results deviate from the actual expression level of genes: (1) distortion of levels during PCR amplification of cDNA mixtures, and (2) statistical sampling variations. Two lines of evidence, however, argue against these artifacts influencing our results. First, the expression patterns of known genes that have been previously independently studied are consistent with those reported here. For example, the EST frequency of S-adenosylhomocysteine hydrolase is significantly increased at the 16-cell embryo stage and decreased again at the blastocyst stage (Table 3). This gene has been identified as the causative gene for the mouse lethal nonagouti (ax) mutation and our results are consistent with the reported expression of this gene in preimplantation stages (Miller et al., 1994). Another example is connexin31, a gapjunction protein. Blastocyst-specific expression based on EST analysis (Table 3) is consistent with the previous report (Reuss et al., 1997). High expression of radixin at the Morula stage is also consistent with its function as a cell adhesion molecule and its reported expression pattern (Funayama et al., 1991), and blastocyst-stage specific appearance of placental lactogen II (Jackson et al., 1986) is also consistent with placenta-specific expression of the gene. Second, reverse transcriptase (RT)-PCR analyses on staged embryos confirmed the expression patterns for selected genes, e.g. the gene Alpha03732 (Fig. 3A). Low levels of transcripts were also detected in unfertilized eggs and fertilized eggs, but transcripts were most abundant at the 2-cell stage, confirming the observed EST frequency that showed 2-cell stage expression of this gene. Gamma-actin as a control showed a comparable level of transcripts in all stages (Table 3, Fig. 3B). Genetic mapping of novel ESTs Primer pairs were designed and synthesized for 4500 ESTs, selected from the large fraction of new ESTs that were not ‘named genes’ in the public sequence database. The PCR products of all primer pairs were tested for sequence polymorphisms between C57BL/6J and M. spretus by a heteroduplex assay (Ko et al., 1998). About 800 primer pairs were found to produce sequence polymorphisms, and these were genotyped on The Jackson Laboratory BSS Interspecific Backcross Panel. The map location of these ESTs, along with known genes (Ko et al., 1994) and ESTs (Ko et al., 1998) that we previously mapped on the same panel, are shown in Fig. 4. Fig. 4 also indicates the expression patterns of individual genes, assessed by counting the representation of these ESTs at each stage of development. More detailed map information, including the raw data and the relative positions of these markers to other markers in this mapping cross, is accessible

through the World Wide Web [http://www.jax.org/resources/ documents/cmdata/bkmap/BSS.html]. It is interesting to note that some genes with similar developmental expression patterns have been shown to cluster on the mouse genetic map. For example, there are clusters of blastocyst-specific genes in chromosomes 1 and 8, and unfertilized egg-specific genes in chromosomes 7 and 16 (Fig. 4). DISCUSSION Large-scale cDNA sequencing projects have successfully produced more than 1 million ESTs (Adams et al., 1991; Hillier et al., 1996; Hwang et al., 1997; Marra et al., 1999, 1998; Okubo et al., 1992). However, the majority of cDNA libraries have been derived from adult organs and tissues. Because many genes are only expressed at limited times and places, and often at low levels, the gene catalogue thus remains incomplete. In particular, limited information has been obtained about transcripts in the stages of preimplantation mammalian development (Adjaye et al., 1997, 1998; Rothstein et al., 1992, 1993; Sasaki et al., 1998). The optimization of a PCR-based cDNA library construction method (Takahashi and Ko, 1994) has provided seven cDNA libraries used here, and although the libraries were not normalized, a high rate of new gene discovery was seen (9718 unique genes from a total of 25,438 ESTs). This very likely reflects the high complexity of mRNA species in preimplantation embryos. Furthermore, about 50% of the 9718 unique genes were seen for the first time in this study, presumably because these preimplantation mammalian embryonic stages have not been extensively used in other EST projects. Discussion of the genes showing very interesting expression patterns is largely outside the scope of this paper, but one can easily illustrate some of the usefulness of the data. For example, ERCC3, which is part of TFIIH that has helicase activity and is involved in base excision repair of transcribed DNA (Weeda et al., 1990), is particularly abundant at the 2cell stage (Table 2). This is concurrent with the initiation of zygotic transcription when many nuclear genes are starting to be expressed. Another example is the BMAL1 gene, which was recently identified as a partner for heterodimer formation with CLOCK genes and plays a critical role in mammalian circadian rhythm (Darlington et al., 1998). Transcripts were present in unfertilized eggs, 2-cell and 16-cell stages (Table 2, http://www.bioa.jst.go.jp/pge/). Although further analyses are required, this pattern suggests that the gene is expressed intermittently, skipping some developmental stages. This finding implies that the timing of cell division at the cleavagestage mouse embryos may be controlled by the same pathway as the circadian rhythm in the adult mouse. The newly mapped genes will provide a valuable resource for positional cloning of mouse genes. Given their substantial homology in gene organization, the mouse data should also help the positional cloning of human genes. In addition, many of these genes are apparently unique to early mammalian embryos. Consequently, the gene-mapping efforts presented here will provide a complement to the ongoing large-scale EST mapping projects in human and mouse. Finally, from the EST PCR primer pairs described here, approximately 2500 are now being mapped on the T31 radiation hybrid panel at the MRC UK

1748 M. S. H. Ko and others Mouse Genome Center (Paul Denny and Steve Brown, personal communication, http://www.mgc.har.mrc.ac.uk/est_maps.htm). The 798 genes mapped here in The Jackson Backcross Panel will help to anchor the genetic and radiation hybrid maps. The map also provides a genome-wide view of the distribution of genes with information on expression levels at each stage (Fig. 4). One significant feature of the map is that genes with similar expression patterns appear to cluster on the mouse genome. This support the previous suggestion that mammalian genomes are evolved so that coexpressed genes often tend to cluster (Ko et al., 1998). Physical proximity may provide the embryos with an efficient means of coordinately regulating the expression of many genes. Two general methods for global expression analyses – a high resolution two-dimensional protein gel analysis and mRNA differential display – have been applied to the preimplantation mouse development. Comprehensive two-dimensional gel analyses during the 1-, 2- and 4-cell stages have identified about 1500 protein spots, some 38 of which show a transient increase in a 2-cell stage-specific manner (Latham et al., 1991). Another study has examined 1674 protein spots between compacted 8cell and blastocyst-stage mouse embryos and identified 43 protein spots that are present only at 8-cell stage and 75 protein spots that are present only at blastocyst-stage (Shi et al., 1994). Because the two-dimensional protein gels represent the protein biosynthesis and/or the modification of pre-existing proteins (Oh et al., 1999), the overall pattern changes could reflect differential regulations at the translational or post-translational level. Another inherent drawback is a difficulty in isolating genes that correspond to each protein spot. In fact, only a few protein spots have been identified at a gene level (Schultz, 1999). In contrast, mRNA differential display has been successfully used to identify some 2-cell, 8-cell and blastocystspecific cDNA fragments (Zimmermann and Schultz, 1994). However, only one new gene (eIF-4C) has been identified as a 2-cell stage specific gene (Davis et al., 1996; Schultz, 1999). The mRNA differential display has inherent difficulties for gene identification, because isolated cDNA fragments are usually too short to provide clear identity and too fragmentary to recover full-length cDNA clones. In this sense, the subtraction cDNA library method has been most successfully used to isolate stagespecific expressed genes (Oh et al., 1999), although it does not provide much information about global changes of gene expression patterns. Four new genes that are present in the 1cell and 2-cell embryos, but are absent in the 8-cell embryos, have been identified in this manner (Oh et al., 1999). Considering these facts, the EST approach may have advantages over the other methods, because (1) cDNA clones are readily available for the detailed analysis of genes; (2) the expression levels of genes are monitored as the relative abundance of mRNAs; and (3) it provides the information about global changes of gene expression patterns. The EST data sets from each stage-specific cDNA library provide a first gene-based index of overall gene expression patterns during preimplantation development. For early cleavage-stage embryos, the data presented here confirm earlier analyses based on smaller numbers of genes (reviewed in Kidder, 1992; Schultz and Heyner, 1992; Watson et al., 1992). For example, the results support the previous inference that most maternally stored mRNAs in unfertilized eggs are degraded by the two-cell stage (Piko and Clegg, 1982). The

individually isolated genes identify many known and unknown genes that may contribute to a more detailed understanding of processes like RNA degradation. Approximately 50% of all the genes in our collection initiated expression at the 1-cell and 2cell stages. This is also consistent with previous studies (Piko and Clegg, 1982). Because the majority of studies of zygotic gene activation have been done by analyzing the transcriptional activity of exogenously injected genes (reviewed in Kaneko and DePamphilis, 1998), endogenous genes found here will provide the additional means with which to analyze the zygotic gene activation. An obvious extension of the studies will be to see whether specific cohorts of genes are activated earlier than others. In contrast, the data presented here would suggest that the paradigm for the late cleavage-stage embryos needs to be revised. The existing paradigm is that although the onset of expression of individual genes is varied, almost all of them continue to be expressed once expression starts (Kidder and McLachlin, 1985; Levy et al., 1986; Kidder, 1992; Schultz and Heyner, 1992; Watson et al., 1992). Such findings have prompted researchers to believe that ‘the transcription of most genes in preimplantation development is not temporally linked with the morphogenetic transitions they participate in’ (Kidder, 1992). However, the new finding here of many apparently stage-specific expressed genes may challenge this conventional view of the regulation of gene expression during early mouse development. Preimplantation development usually takes 4 to 6 days, and each cell division is therefore rather slow, and each cell cycle/division has time to generate new and unusual gene expression patterns from the selective destruction and activation of many genes. The existence of many stage-specific expressed genes has two primary implications. First, developmental processes may lead to significant differences in gene expression in the transition from 2 cells to 8 cells, even though the process appears to be simply a division of cells. Second, stage-specific expressed genes may actively promote the advancement of embryos from one stage to the next. Considering the rapid and selective turnover of these particular mRNAs, there is likely to be selection for their rapid degradation. The requirement of function of that particular gene is apparently transient, which suggests a ‘hit-and-run’ type of mechanism for expression of cascades of genes. It will be of interest to see, for example, if specific inactivation of phase-specific genes causes arrest of development at stages of oocyte formation or cleavage. We would like to thank Drs David Schlessinger, Dan Longo, and Ramaiah Nagaraja for critical reading of the manuscript, Ms Shoshana Stern for assisting with the art work, and Ms Mary Barter for assisting with the genetic map figure. This work was supported by Science and Technology Agency in Japan through the ERATO/JST program, and received additional support from NICHD/NIH grant (RO1HD32243) and the NIA/NIH intramural research program.

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