BCL7A. B-cell CLL/lymphoma 7A. 0.0004. 497.5. 5.6. 0.300. â. 32. 17. 0.0754. 88.07. 0.0576. 251.9. +. 53. 24. 24.13. 237.1. CTLA4. Cytotoxic T-lymphocyte.
Original Article
Gene expression profiling reveals differences in microenvironment interaction between patients with chronic lymphocytic leukemia expressing high versus low ZAP70 mRNA Basile Stamatopoulos,1 Benjamin Haibe-Kains,2,3 Carole Equeter,2 Nathalie Meuleman,1 Anne Sorée,1 Cécile De Bruyn,1 Delphine Hanosset,1 Dominique Bron,1 Philippe Martiat1 and Laurence Lagneaux1 1
Laboratory of Experimental Hematology, Faculty of Medicine, Institut Jules Bordet, Université Libre de Bruxelles (ULB), Brussels; Functional Genomics and Translational Research Unit, Faculty of Medicine, Institut Jules Bordet, Université Libre de Bruxelles (ULB), Brussels, and 3Machine Learning Group, Faculty of Sciences, Université Libre de Bruxelles (ULB), Brussels, Belgium
2
ABSTRACT Background Zeta-associated protein 70 (ZAP70) is a widely recognized prognostic factor in chronic lymphocytic leukemia, but mechanisms by which its higher expression leads to a poor outcome must still be fully explained. Design and Methods In an attempt to unveil unfavorable cellular properties linked to high ZAP70 expression, we used gene expression profiling to identify genes associated with disparities in B cells from chronic lymphocytic leukemia patients expressing high versus low ZAP70 mRNA, measured by quantitative real-time PCR. Two groups of 7 patients were compared, selected on the basis of either high or low ZAP70 mRNA expression. Results Twenty-seven genes were differentially expressed with an FDR
