In this study the genetic diversity in Turkish native sheep breeds was investigated based on microsatellite DNA loci. In total, 423 samples from 11 native.
GENETIC DIVERSITY OF NATIVE AND CROSSBREED SHEEP BREEDS IN ANATOLIA
A THESIS SUBMITTED TO THE GRADUATE SCHOOL OF NATURAL AND APPLIED SCIENCES OF MIDDLE EAST TECHNICAL UNIVERSITY
BY EVREN KOBAN
IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY IN DEPARTMENT OF BIOLOGY
DECEMBER 2004
Approval of the Graduate School of Natural and Applied Sciences
________________________ Prof. Dr. Canan ÖZGEN Director I certify that this thesis satisfies all the requirements as a thesis for the degree of Doctor of Philosophy.
________________________ Prof. Dr. Semra KOCABIYIK Head of the Department This is to certify that we have read this thesis and that in our opinion it is fully adequate, in scope and quality, as a thesis for the degree of Doctor of Philosophy.
________________________ Prof. Dr. nci TOGAN Supervisor Examining Committee Members
Prof. Dr. Zeki KAYA
(METU, BIO)
_________________
Prof. Dr. nci TOGAN
(METU, BIO)
_________________
Prof. Dr. M. hsan SOYSAL
(Trakya Ünv., TZF)
_________________
Prof. Dr. Mehmet N ZAMLIO LU (Selçuk Ünv., Vet. Fak.) _________________ Prof. Dr. Okan ERTU RUL
(Ankara Ünv., Vet. Fak.) _________________
I hereby declare that all information in this document has been obtained and presented in accordance with academic rules and ethical conduct. I also declare that, as required by these rules and conduct, I have fully cited and referenced all material and results that are not original to this work.
Evren KOBAN
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ABSTRACT GENETIC DIVERSITY OF NATIVE AND CROSSBREED SHEEP BREEDS IN ANATOLIA
Koban, Evren Ph.D., Department of Biology Supervisor: Prof. Dr. nci Togan December 2004, 125 pages
In this study the genetic diversity in Turkish native sheep breeds was investigated based on microsatellite DNA loci. In total, 423 samples from 11 native and crossbreed Turkish sheep breeds (Akkaraman, Morkaraman, Kıvırcık, vesi, Da lıç, Karayaka, Hem in, Norduz, Kangal, Konya Merinosu, Türkgeldi) and one Iraqi breed (Hamdani) were analyzed by sampling from breeding farms and local breeders. After excluding close relatives by Kinship analysis, the genetic variation within breeds was estimated as gene diversities (HE), which ranged between 0.686 and 0.793. The mean number of observed alleles (MNA) ranged between 5.8 and 11.8. The allele frequency distribution across Turkey showed no gradient from east to west expected in accordance with the Neolithic Demic Diffusion model. The differentiation between different samples of Akkaraman, Da lıç and Karayaka breeds was tested by FST index. Akkaraman1 sample from the breeding farm was iv
significantly (P
