Abstracts / Journal of Biotechnology 185S (2014) S37–S125
in the studied digestive organs. Results showed the possibility of using the “Ingo-2” in the practical medicine as a new nanosorbent. http://dx.doi.org/10.1016/j.jbiotec.2014.07.163 A new CT procedure for the study of the elbow in the dog Carla Murino 1,∗ , Giuseppina Mennonna 1 , Bruna Santangelo 2 , Francesco Lamagna 2 , Caterina Balestriere 2 , Leonardo Meomartino 1 1 Radiology Veterinary Centre of University of Naples Federico II, Naples, Italy 2 Department of Veterinary Medicine and Animal Production of University of Naples Federico II, Naples, Italy
E-mail address:
[email protected] (C. Murino). Introduction: Previous described canine elbow-CT procedures need the head pulled laterally and caudally. This positioning is stressful for the neck and potentially dangerous in breeds prone to the Wobbler syndrome. Aim: To develop a new non-stressful procedure for canine elbow-CT. Materials and methods: Prospectively, from January 2013, two elbow-CT procedures were used. The described procedure (DP): sternal recumbency with laterally and caudally pulled head and cranially extended forelimbs. The new procedure (NP): lateral recumbency, with forelimbs cranially pulled, very close one to each other and to the neck, in order to obtain the smallest field of view (FOV) and mean pixel dimension (MPD). CT procedures were compared for spatial resolution and presence of artefacts. Results: 18 elbows, from 9 dogs, were included. Mean FOV was: NP 126.6 mm (±14.1); DP 103.3 (±5.16); mean MPD was: NP 0.25 mm (±0.03); DP 0.21 mm (±0.01). FOV and MPD were significantly larger in NP. Streak artefacts were absent in both procedures. Discussion and conclusion: compared to the DP, the NP has a significantly larger FOV and MPD, but a simpler and safer positioning, without streak artefacts. Furthermore, NP permits a more symmetrical positioning of the elbows and therefore to visualize them simultaneously. http://dx.doi.org/10.1016/j.jbiotec.2014.07.164 Genetic diversity of Romanian Grey Steppe cattle based on milk protein polymorphism Daniela Elena Ilie 1,∗ , Steofil Creanga 2 , Andrei Cristian Gradinaru 3 , Silviu Ionut Bors 2 , Dan Lucian Dascalu 2 , David Chirila 4 , Ada Cean 1 1 Research and Development Station for Bovine, Arad, Romania 2 Research and Development Station for Bovine, Iasi, Romania 3 University of Agricultural Sciences and Veterinary Medicine of Iasi, Iasi, Romania 4 Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Timisoara, Romania
E-mail address:
[email protected] (D.E. Ilie). The aim of this study was to determine the polymorphism of the most important lacto-proteins in Romanian Grey Steppe breed, currently included in a genetic resource preservation program. DNA samples from 55 Romanian Grey Steppe individuals, including six bulls, were genotyped for kappa-casein (-CN) and betalactoglobulin (-LG) and only 50 samples for alpha-lactalbumin
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(␣-LA), using the PCR-RFLP technique. The allele and genotype frequencies were calculated by direct counting, and the HardyWeinberg equilibrium was tested using the chi-square test (2 ). In addition, the homozygosity coefficient, based on homozygotic genotype frequencies, was statistically interpreted. A and B variants were identified for -CN and -LG, whereas only the B allele was identified for ␣-LA. The frequencies for A and B alleles were 0.464 and 0.536 for -CN, and 0.409 and 0.591 for -LG, respectively. The AB genotype was the most prevalent for both -CN and -LG (0.491 and 0.564), whereas the AA genotype displayed the lowest frequencies (0.218 and 0.127) for the same proteins. The results of the chi-square test for Hardy-Weinberg equilibrium showed that the investigated individuals are in genetic equilibrium. The calculated homozygosity coefficient was 22.03%, which is representative for every Hardy-Weinberg population. Acknowledgements This work was financed through PNII-PCCA-120/2012 program. http://dx.doi.org/10.1016/j.jbiotec.2014.07.165 Using geographical information system for spatial evaluation of canine disc herniations Constantin Daraban 1 , Carla Murino 2,∗ , Giuseppe Marzatico 2 , Giuseppina Mennonna 2 , Gerardo Fatone 2 , Fabiana Micieli 2 , Vasile Vulpe 1 , Leonardo Meomartino 2 1
Faculty of Veterinary Medicine, Iasi, Romania Department of Veterinary Medicine and Animal Production, Naples, Italy 2
E-mail address:
[email protected] (C. Murino). Background: Geographical Information System (GIS) is used in analyzing and displaying referenced information. GIS applications have never been reported in Veterinary Imaging. Aim: To develop a GISbased vertebral canal (VC) map useful for spatial evaluation of extruded disc herniation (EDH) in dogs. Methods: A GIS software (ArcGIS® ) was used to create 2D and 3D maps in which VC surface was divided in polygons by lines representing the latitude and longitude. Depending on dislocation, EDH could be localized in one of the above regions. The actual dislocations of herniated disc material were assessed using a series of CT studies. Choropleth maps were realized based on frequency of EDH dislocation. Results: A cylindrical model of the VC with 78 latitudinal and 8 longitudinal lines dividing 624 polygons was created. Dislocation of EDH was assessed in 142 dogs with 177 EDH. Most of EDHs were located on the transitional regions (cervico-axial; cervico-thoracic; thoracolumbar; lumbo-sacral) at level of ventro-cranial and ventro-central polygons. Discussions: choropleth maps highlighted both the distribution and the dislocation pattern of EDH throughout the VC. Conclusion: GIS proved to be a valuable tool in analyzing dog’s EDH. Further studies are required for biomechanical analysis of EDH’s dislocation pattern. http://dx.doi.org/10.1016/j.jbiotec.2014.07.166
