Aug 7, 2013 - tigated for over 100 years, and Aeromonas hydrophila, which belongs to the .... Aeromonas salmonicida subsp. salmonicida A449 (GenBank.
Appl Microbiol Biotechnol DOI 10.1007/s00253-013-5189-y
GENOMICS, TRANSCRIPTOMICS, PROTEOMICS
Genomic study of polyhydroxyalkanoates producing Aeromonas hydrophila 4AK4 Xue Gao & Jia Jian & Wen-Jie Li & Yu-Cheng Yang & Xiao-Wen Shen & Zhi-Rong Sun & Qiong Wu & Guo-Qiang Chen
Received: 25 June 2013 / Revised: 7 August 2013 / Accepted: 11 August 2013 # Springer-Verlag Berlin Heidelberg 2013
Abstract The complete genome of Gram-negative Aeromonas hydrophila 4AK4 that has been used for industrial production of poly(3-hydroxybutyrate-co -3-hydroxyhexanoate) was sequenced and annotated. Its chromosome is 4,527,993 bp in size encoding 4,272 genes, including 28 rRNA genes and 104 tRNA genes. Comparative analysis indicated that genome of A. hydrophila 4AK4 was similar to that of the A. hydrophila ATCC 7966T, an intensively studied aeromonad for its pathogenicity related to its genomic information. Genes possibly coming from other species or even other genus were identified in A. hydrophila 4AK4. A large number of putative virulent genes were predicted. However, a cytotonic enterotoxin (Ast) is absent in A. hydrophila 4AK4, allowing the industrial strain to be different from other A. hydrophila strains, indicating possible reduced virulence of strain 4AK4, which is very important for industrial fermentation. Genes involved in polyhydroxyalkanoate (PHA) metabolism were predicted and analyzed. The resulting genomic information is useful for improved production of PHA via metabolic engineering of A. hydrophila 4AK4. Keywords Aeromonas hydrophila . PHB . Genome . Virulence . Polyhydroxyalkanoates
Xue Gao and Jia Jian contributed equally to this paper. X. Gao : J. Jian : W.
