Product name. Glycerol Assay Kit (Cell-Based). Tests. 1 x 96 well plate. Sample
type. Cell culture supernatant, Adherent cells, Cell culture media. Assay type.
Product datasheet
Glycerol Assay Kit (Cell-Based) ab133130 2 Images Overview Product name
Glycerol Assay Kit (Cell-Based)
Sample type
Cell culture supernatant, Adherent cells, Cell culture media
Assay type
Cell-based
Species reactivity
Reacts with: Human Predicted to work with: Mammal
Product overview
Glycerol Assay Kit (Cell-Based) (ab133130) provides a convenient tool for studying triglyceride/fatty acid cycling and its regulation in adipocytes or hepatocytes. This kit will allow investigators to screen compounds involved in lipid storage and metabolism. Chloroquine is included in the kit as a positive control for screening compounds that induce lipid droplet accumulation and free glycerol release from hepatocytes.
Notes
In mammals, triglycerides are constantly synthesized from fatty acids and segregated into cytosolic lipid droplets, mainly in adipocytes, as the major energy storage depot. During fasting, triglycerides stored in adipose tissue and liver are hydrolyzed by hormone-sensitive lipase and adipose triglyceride lipase to produce free fatty acids and glycerol. Triglyceride/fatty acid cycling is important in metabolic regulation and heat production, and is highly regulated by enzymes such as phosphenolpyruvate carboxykinase (PEPCK) and lipases. Quantitative changes in the triglyceride/fatty acid cycle have been related to the increased metabolic rate of cachectic patients with esophageal cancer and to metabolic syndrome. Abnormal triglyceride accumulation in the form of lipid droplets can occur in adipocytes and/or hepatocytes of obese mammals. In vitro, dramatic lipid accumulation can be observed in well-differentiated 3T3-L1 cells, or HepG2 cells treated with steatosis-inducing compounds such as chloroquine. Triglycerides stored in these lipid droplets can be hydrolyzed into free fatty acids and glycerol which are subsequently released into the surrounding environment. The amount of glycerol released into the medium is proportional to the triglyceride/fatty acid cycling rate.
Tested applications
Suitable for: Functional Studies
Properties Storage instructions
Store at -20°C. Please refer to protocols.
Components
1 kit
Chloroquine Positive Control (25 mM)
1 vial 1
Components
1 kit
Free Glycerol Assay Reagent (10X)
2 vials
Glycerol Standard Solution
1 vial
Relevance
Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development.
Applications Our Abpromise guarantee covers the use of ab133130 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Functional Studies
Notes Use at an assay dependent concentration.
Images
Glycerol release from HepG2 cells treated with chloroquine. HepG2 cells were seeded at a density of 104 cells/well in a 96-well plate and grown overnight in a 37°C incubator. The next day, cells were treated with vehicle or different doses of chloroquine for 24 hours. At the end of this incubation, supernatants were collected Glycerol Assay Kit (Cell-Based) (ab133130)
and analyzed for free glycerol according to the procedure described in the protocol.
2
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Glycerol Assay Kit (Cell-Based) (ab133130)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3
