(LH) and follicle-stimulating hormone. (FSH);. 2) hypothalamic content of gonadotropin releasing hormone. (GnRH); and. 3) numbers of receptors for GnRH were.
BIOLOGY
OF
REPRODUCTION
25,938-944(1981)
GnRH-Receptor Interaction. VI. Effect of Progesterone and Estradiol on Hypophyseal Receptors for GnRH, and Serum and Hypophyseal Concentrations of Gonadotropins in Ovariectomized Ew& G. E. MOSS,2
M. E. CROWDER
Department
of Physiology
and and
T. M. NETT3 Biophysics,
Colorado State University, Fort Collins, Colorado 80523
ABSTRACT The effects of estradiol and progesterone on 1) hypophyseal and serum concentrations of luteinizing hormone (LH) and follicle-stimulating hormone (FSH); 2) hypothalamic content of gonadotropin releasing hormone (GnRH); and 3) numbers of receptors for GnRH were examined in ovariectomized ewes. Three days after ovariectomy, ewes were given 16 mg progesterone (n 5), 0.7 mg estradiol (n 5) or 16 mg progesterone plus 0.7 mg estradiol (n 5) s.c. in ethanol twice daily for 3 weeks prior to slaughter. Ten control ewes were given injections of ethanol according to the same schedule. Five intact ewes were killed 12 days after an induced ovulation. Blood samples were collected daily for 4 days prior to slaughter, and the serum was analyzed for concentrations of progesterone, estradiol, LH, and FSH. Hypothalami were removed after sacrifice and the amount of GnRH present in the preoptic area, median eminence, and hypothalamus proper was determined. In addition, the pituitary gland from each animal was removed and the number of receptors for GnRH and concentrations of LH, FSH, and prolactin were measured. Steroid treatment produced concentrations of estradiol and progesterone similar to those observed during late pregnancy in ewes. Administration of either progesterone or estradiol, suppressed (P
