Greener approach for synthesis of antibacterial silver

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a Department of Biotechnology, School of Bioengineering, SRM University, Chennai 603 203, .... microscopy (TEM) using JEOL 2100 instrument (with an accel-.
Industrial Crops and Products 66 (2015) 103–109

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Greener approach for synthesis of antibacterial silver nanoparticles using aqueous solution of neem gum (Azadirachta indica L.) Palaniyandi Velusamy a,∗ , Jayabrata Das a , Raman Pachaiappan a , Baskaralingam Vaseeharan b , Kannaiyan Pandian c a

Department of Biotechnology, School of Bioengineering, SRM University, Chennai 603 203, Tamil Nadu, India Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, Tamil Nadu, India c Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India b

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Article history: Received 25 June 2014 Received in revised form 16 December 2014 Accepted 19 December 2014 Keywords: Green synthesis Neem gum Silver nanoparticles Autoclave DNA degradation Antibacterial activity

a b s t r a c t A simple method for the green synthesis of silver nanoparticles (AgNPs) using autoclave assisted gum extract of neem (Azadirachta indica) has been investigated for the first time. Silver nanoparticles were formed due to reduction of silver nitrate solution when mixed with the gum extract after autoclaving at 121 ◦ C and 15 psi. The UV–vis absorption spectrum of the biologically reduced reaction mixture showed the surface plasmon peak at 418 nm which is characteristic peak of silver nanoparticles. The functional biomolecules present in the gum extract and the interaction between the nanoparticles were identified by the Fourier transform infrared spectroscopy (FTIR) analysis. Average diameter of the synthesized nanoparticles was found to be