against Age-Related NLRP3 Inflammasome Activation and Immune Senescence. Olga Spadaro, Emily L. Goldberg, Christina D. Camell, Yun-Hee Youm, John J.
Cell Reports, Volume 14
Supplemental Information
Growth Hormone Receptor Deficiency Protects against Age-Related NLRP3 Inflammasome Activation and Immune Senescence Olga Spadaro, Emily L. Goldberg, Christina D. Camell, Yun-Hee Youm, John J. Kopchick, Kim Y. Nguyen, Andrzej Bartke, Liou Y. Sun, and Vishwa Deep Dixit
A
WT (3m)
Ghr-/- (3m)
WT (36m)
Ghr-/- (36m)
CD8 CD4
B
CD8+
Figure S1 Related to Figure 1, Spadaro et al
r -/-
LOD Gh
r -/Gh
WT
LOD
IL-5 pg/ml
E
IL-4 pg/ml r -/Gh
WT
r -/Gh
WT
r -/-
3 month
D
TNFα pg/ml
C
Gh
WT
r -/Gh
36 month
WT
3 month
WT
r -/Gh
WT
% of gated
% of gated
CD4+
36 month
-
Gh r-/
T
W
3 month
W
T
-
30 month
3 month
M1
Figure S2 Related to Figure 2, Spadaro et al
M1
W
T
30 month
W
3 month
M2
T 30 month
Chi3l3
30 month
3 month
Gh r-/ -
r-/ -
(Relative expression)
Gh r-/ -
*
NT
TNFα M0
Arg-1
W
T
M2 W T
3 month
M0
Retnla
30 month
r-/ -
WT Gh r-/ W T Gh r-/ WT Gh r-/ W T Gh r-/ -
Gh
W T
(Relative expression)
(Relative expression)
Casp-1
Gh
LPS
-
T
T
3 month 30 month 3 month 30 month
r-/
W
W
M0
(Relative expression)
r-/ -
Gh r-/ -
30 month
Gh
3 month
-
Ccl24 LPS
Gh r-/
3 month 30 month 3 month 30 month
T
(Relative expression)
M0
r-/ -
IL-12b
Gh
LPS
Gh r-/ -
T
WT Gh r-/ W T Gh r-/ WT Gh r-/ WT Gh r-/ -
Gh
W T
Gh r-/ -
3 month
W
(Relative expression)
IRF5
T
-
30 month
W
r-/
T
(Relative expression)
r-/ -
Asc
Gh
iNOS LPS
-
3 month
W
W
M0
Gh r-/
(Relative expression)
LPS
T
30 month 30 month
W
(Relative expression)
M0
r-/ -
IL-1b
-
3 month
Gh
Ccl22 W
r-/
LPS
Gh r-/ -
WT Gh r-/ W T Gh r-/ WT Gh r-/ W T Gh r-/ -
3 month 30 month 3 month 30 month
T
Gh
3 month
T
(Relative expression)
Gh
W T
(Relative expression)
Nlrp3
Gh r-/
T
W
M0
T
(Relative expression)
30 month
W
(Relative expression)
W r-/ -
C
Gh
WT Gh r-/ W T Gh r-/ WT Gh r-/ W T Gh r-/ -
(Relative expression) 3 month
-
E W
r-/
Gh r-/ -
T
W
D T
Gh
(Relative expression)
(Relative expression)
A B NLRC4
*
M0
30 month
WT 3Mo WT 30Mo
LPS M1
M1
IL-6 LPS
3 month 30 month 3 month 30 month
LPS
IL-4R
1000 750
500 400 300 200
500 400 300 200
100
100
Lane 4
Lane 3
Lane 2
Lane 1 1000 750
Figure S3 Related to Figure 3, Spadaro et al
Subcutaneous Adipose Tissue WT (12m)
Ghr-/- (12m)
Gh r -/-
WT
# cells/g
MHCII+
B220+ MHCII+
B220
Visceral Adipose Tissue Ghr-/- Urate (12m)
B220+ MHCII+
B220+
Figure S4 Related to Figure 4, Spadaro et al
SHAM
r -/-
WT
r -/Gh
WT
MHCII+
*
Gh
WT Urate (12m)
# cells/g
WT-Sham (12m)
MSU
1
Figure S1. Related to Figure 1 CD4 and CD8 T cells subsets in the splenocytes of 3Mo-36Mo WT and Ghr -/- mice represented as FACS staining (A) and as percentages of total gated cells (B). (C-E) Luminex analysis of cytokine secretion from CD4+ CD44+ effector-memory sorted and activated T cells from splenocytes in 12 month WT and Ghr -/- mice siblings. (one dot represents one mouse; n=3/group; error bars are representative as mean ± SEM; *p
