propione. Boutdoba. B2bre vibac. K2 kineocoC. Atlanthroba. 2leifsoni me marbeterium. 2 Adidto the ther mobi. Ravermiti. 200elicol. Pjanibact no wardio renkia.
Hayward et al. 2008 thermophi
S. thermophilum glnA
100
99
100 100 83
83 100 63
100
96
100 71 100
100
89 100 88
100
53 83
100
100
100
94 100 100
69 82
94
94 91 100 100
100
100 59 65 58
100
64
53 100 92 89 100
100
90 59
100
100
97
100 50
100 63
3rankia Janibacter 3anibacte M. tuberculosis 337Rv M. avium 3vium glnA3 M. flavescens 3lavescen Mycobacterium 3MS KMS M. smegmatis 3m egmatis M. vanbaalenii 3anbaalen Rhodococcus 3hodococc Rhodococcus 4rhodococ Frankia 4frank ia M. tuberculosis 4h37rv M. avium 4Avium M. marinum 4marine M. flavescens 4flavensc Mycobacterium 4KMS KMS M. smegmatis 4smegm ati glnA4 M. vanbaalenii 4vanbaale S. avermitilis 4avermiti S. coelicolor 4coelicol Nocardioides 4Nocardio A. cellulolyticus 4acidothe Janibacter 4Janibact K. radiotolerans 4kineococ B. linens 4Brevibac Frankia
100
1bifidoba 1frank ia 1Thermobi 1acidothe 1salinisp 1coelicol 1avermiti 1nocardio 1propione 1leprae 1tuberc ul 1Africanu 1avium 1ulcerans 1microtti marinum a KMS a1 1vanbaale 1flavesce 1sm egmati 1Rhodococ 1Nfarcini 1diphther 1jeikeium 1efficien 1glutam ic 1janibact 1radiotol 1brevibac 1Arthroba 1m arine 1leifsoni
B. longum Frankia T. fusca A. cellulolyticus S. tropica S. coelicolor S. avermitilis Nocardioides P. acnes M. leprae M. tuberculosis 100 M. africanum M. avium M. ulcerans 100 98 M. microtti M. marinum Mycobacterium KMS M. vanbaalenii M. flavescens M. smegmatis Rhodococcus N. farcinica C. diphtheria C. jeikeium C. efficiens C. glutamicum B. linens K. radiotolerans Janibacter Arthrobacter marine actinobacterium L. xyli
2leprae 2avium 2tubercul 2africanu 2microtti 2ulcerans 2marinum 2kms 2smegmati 2flavesce 2vanbaali Nocardia farcinica 2farcinic Rhodococcus 2rhodococ C. jeikeium 2jeikeium C. diphtheriae 2diphther C. efficiens 2efficien C. glutamicum 2glutamic P. acnes 2propione B.2bifidoba longum B. linens 2brevibac K. radiotolerans 2kineococ Arthrobacter 2arthroba L.2leifsoni xyli marine actinobacterium 2m arine A. cellulolyticus 2Acidothe T. fusca 2thermobi S. avermitilis 2avermiti S. 2ccoelicolor oelicol Janibacter 2janibact 2nocardio Nocardioides Frankia 2frankia S. tropica 2salinisp M. leprae M. avium M. tuberculosis M. africanum M. microtti M. ulcerans M. marinum Mycobacterium KMS M. smegmatis M. flavescens M. vanbaalenii
glnA2
glnA1
0.1
Supplementary Figure 1: A phylogeny of all Actinobacteria glnA protein sequence homologs were constructed using the glnA sequence of Symbiobacterium thermophilum as out-group. This reconstruction highlights the clustering and evolutionary relationships of the glnA1, glnA2, glnA3 and glnA4 sequences, and shows that the glnA3 and glnA4 sequences are closely related and in turn more related to the glnA2 sequence than the glnA1 sequence. Percentage bootstrap values are shown.
