m-AMSA µM. Relative survival %. Ubiquitin conjungation / hydrolysis. 0. 1. 2. 3. 4. 10-1. 100. 101. 102. WT, N = 10. âubc4, N = 3. âubc13, N = 3. âdoa4, N = 3.
Homologous recombination 1
Homologous recombination 1 10 2
10 1
WT, N = 10 ∆rad52, N = 3 ∆rad51, N = 4 ∆rad57, N = 3 ∆rad55, N = 3
10 0 10 -1 10 -2
Relative survival %
Relative survival %
10 2
10 -1
∆rad57, N = 4 ∆rad55, N = 3
10 -3 0
1
2
3
4
0
5
10
15
ICRF-187 mM
m-AMSA µ M
Homologous recombination 2
Homologous recombination 2
20
10 2
10 1
WT, N = 10 10 0
∆rad54, N = 3 ∆rad59, N =3 ∆dmc1, N = 3 ∆sae2, N = 3
10 -1 10 -2
Relative survival %
10 2
Relative survival %
10 0
∆rad52, N = 4 ∆rad51, N = 3
10 -2
10 -3
10 1
WT, N = 9 ∆rad54, N = 3 ∆rad59, N = 3 ∆dmc1, N = 3 ∆sae2, N = 4
10 0 10 -1 10 -2
10 -3
10 -3 0
1
2
3
4
0
5
10
15
ICRF-187 mM
m-AMSA µ M
Homologous and non-homologous recombination
Homologoue and non-homologous recombination
20
10 2
10 1 10 0
WT, N = 10 ∆rad50, N = 6 ∆mre11, N = 3 ∆xrs2, N = 3
10 -1 10 -2 10 -3
Relative survival %
10 2
Relative survival %
WT, N = 9
10 1
10 1 10 0
WT, N = 9 10 -1
∆rad50, N = 3 ∆mre11, N = 3 ∆xrs2, N = 3
10 -2 10 -3
10 -4
10 -4 0
1
2
ICRF-187 mM
3
4
0
5
10
m-AMSA µ M
15
20
NHEJ
NHEJ 10 2
10 1
10 0
WT, N = 10
10 -1
∆yku70, N = 3 ∆yku80, N = 3
Relative survival %
Relative survival %
10 2
0
1
2
3
0
5
10
15
ICRF-187 mM
m-AMSA mM
Checkpoints 1
Checkpoints 1
20
10 2
WT, N = 10
10 1
∆mec3, N = 7 ∆ddc1, N = 3 ∆rad17, N = 3
10 0
10 -1
Relative survival %
Relative survival %
WT, N = 9 ∆yku70, N = 3 ∆yku80, N = 3
10 -1
4
10 2
10 -2
WT, N = 9 10 1
∆mec3, N = 3 ∆ddc1, N = 3 ∆rad17, N = 3
10 0
10 -1
10 -2 0
1
2
3
4
0
5
10
15
ICRF-187 mM
m-AMSA µ M
Checkpoints 2
Checkpoints 2
10 2
20
10 2
10 1
WT, N = 10 ∆rad9, N = 3 ∆tel1, N = 3 ∆chk1, N = 3
0
10 -1
Relative survival %
Relative survival %
10 0
10 -2
10 -2
10
10 1
10 1
10
WT, N = 9 ∆rad9, N = 3 ∆tel1, N = 3 ∆chk1, N = 3
0
10 -1 0
1
2
ICRF-187 mM
3
4
0
5
10
m-AMSA µ M
15
20
Post replication repair
Post replication repair 10 2
WT, N = 10
10 1
∆rad6, N = 5 ∆rad18, N = 8 ∆rev1, N = 3 ∆rev3, N = 3
10 0
10 -1
Relative survival %
Relative survival %
10 2
10 1
WT, N = 10 ∆rad6, N = 4 ∆rad18, N = 3 ∆rev1, N = 4 ∆rev3, N = 3
10 0
10 -1
10 -2
10 -2 0
1
2
3
0
4
5
10
10 2
Relative survival %
Relative survival %
10 2
10 1
WT, N = 10 ∆rad1, N = 5 ∆rad14, N = 3
0
10 -1
10 1
WT, N = 9 10
∆rad1, N = 3 ∆rad14, N = 3
0
10 -1 0
1
2
3
4
0
5
10
15
ICRF-187 mM
m-AMSA µ M
Base excision repair
Base excision repair
20
10 2
10 1
WT, N = 10 ∆apn1, N = 3
10 0
Relative survival 5
10 2
Rerlative Survival %
20
NER
NER
10
15
m-AMSA µ M
ICRF-187 mM
10 1
10 0
WT, N = 9 ∆apn1, N = 3
10 -1
10 -2
10 -1 0
1
2
ICRF-187 mM
3
4
0
5
10
m-AMSA µ M
15
20
Mismatch Repair
Mismatch Repair 10 2
10 1
WT, N = 10 10
10
∆mhl1, N = 4 ∆pms1, N = 3 ∆msh2, N = 3 ∆msh3, N = 4
0
-1
0
1
2
3
Relative survival %
Relative survival %
10 2
WT, N = 9 10
10
∆mlh1, N = 3 ∆pms1, N = 3
0
∆msh2, N = 4 ∆msh3, N = 4
-1
4
0
5
15
m-AMSA µ M
Membrane transport
Membrane transport
20
10 1
WT, N = 10 ∆atr1, N = 3 ∆pdr5, N = 6 ∆yor1, N = 3
10 0
Relative survival %
10 2
10 -1
10 1
10
WT, N = 9 ∆atr1, N = 5 ∆pdr5, N = 3 ∆yor1, N = 3
0
10 -1
0
1
2
3
4
0
5
10
15
20
m-AMSA µ M
ICRF-187 mM
Ubiquitin conjungation / hydrolysis
Ubiquitin conjungation / hydrolysis
10 2
10 2
WT, N = 10 ∆ubc4, N = 3 ∆ubc13, N = 3 ∆doa4, N = 3 ∆qri8, N = 3
10 1
10 0
Relative survival %
Relative survival %
10
ICRF-187 mM
10 2
Relative survival %
10 1
WT, N = 9 ∆ubc4, N = 3 ∆ubc13, N = 3 ∆doa4, N = 3 ∆qri8, N = 3
10 1
10 0
10 -1
10 -1 0
1
2
ICRF-187 mM
3
4
0
5
10
m-AMSA mM
15
20
Ribonucleotide-reductase regulation
Ribonucleotide-reductase regularion
Relative survival %
10 1
WT, N = 10
10
∆rnr3, N = 3 ∆sml1, N = 3
0
10 -1
Relative survival %
10 2
10 2
10 1
WT, N = 9 ∆rnr3, N = 3 ∆sml1, N = 3
10 0
10 -1 0
1
2
ICRF-187 mM
3
4
0
5
10
m-AMSA mM
15
20
