Hydrolysis of Phosphate Ester Catalyzed by Inorganic

TABLES

Hydrolysis of Phosphate Ester Catalyzed by Inorganic Iron Oxide Nanoparticles Acting as Biocatalysts

Xiao-Lan Huang*.

Table 1 Michaelis-Menten constant (Km) and maximum velocity (Vm) of different phosphorus in a DMT-IO solution (made by Fe(NO3)3 9H2O and NaOH, aged 2 week at 80oC, IO-D). Table 2 Hydrolysis rate (k) of 20 μM G6P and ATP in different DMT-IO solutions Table 3 Effect of storage conditions on the catalysis activity of iron oxide (IO-F) nanoparticle solutions Table 4 Comparison of catalytic parameters of nature peroxidase and different nanozymes

Table 1 Michaelis-Menten constant (Km) and maximum velocity (Vm) of different phosphorus in a DMT-IO solution (made by Fe(NO3)3 9H2O and NaOH, aged 2 week at 80oC, IO-D). Phosphate source G2P G6P ATP Polyphosphate ( poly-Pi) Pyrophosphate (PPi)

Vm (nM S-1) 2.0 3.2 0.9 1.1 2.2

Km ( M) 7.0 8.3 9.2 5.5 1.3

Range ( M) 6 to 200 5 to 100 5 to 50 5 to25 5 to 25

r2 0.99 0.99 0.99 1.00 0.98

Table 2 Hydrolysis rate (k) of 20 μM G6P and ATP in different DMT-IO solutions*

Fe source Fe(NO3)3 Fe(NO3)3 Fe(NO3)3 Fe(NO3)3 FeCl3 Fe(NO3)3 FeCl3 Fe(NO3)3 FeCl3

Manufacturer J T Baker J T Baker J T Baker J T Baker J T Baker Riedel-de Haen Riedel-de Haen Riedel-de Haen Riedel-de Haen

Aged temperature (o C) 5 22 50 80 25 50 50 80 80

Iron oxide (IO) A B C D E H F L G

20 M G6P K (s-1)

t0.5 (h)

1.69E-05 7.03E-05 5.95E-05 1.06E-04 1.38E-05 4.85E-05 2.74E-05 8.07E-05 5.41E-05

11 2.7 3.2 1.8 14 4.0 7.0 2.4 3.6

20 M ATP K (s-1) 1.96E-05 4.04E-05 3.77E-05 7.17E-05 7.00E-06 2.77E-05 1.21E-05 4.58E-05 3.87E-05

* The hydrolysis reaction rate of 20 μM G6P and ATP in DIW is 1.84 x 10-8 and 2.07 x 10-8 s-1, and the corresponding half time is 10450 and 9290 hours.

t0.5 (h) 9.8 4.8 5.1 2.7 28 6.9 16 4.2 5.0

Table 3 Effect of storage conditions on the catalysis activity of iron oxide (IO-F) nanoparticle solutions * 20 MATP

20m G6P Treatment

60min IP (M)

11d: at 22 oC 11 d at 4 oC o

5d frozen (-18 C) and 6 d at 4 oC 5d at 4 oC and 6 d at 22 oC 5d at 50oC and 6 d at 22 oC 5d at 50 oC and 6 d at 4 oC 9d at 50 oC and 2d at 22 oC

300 min IP (M)

Average

Stdev

Average

Stdev

t0.5 ( h)

60min IP (M)

300 min IP (M)

Average

Stdev

Average

Stdev

t0.5 ( h)

11R

5.49

0.004

13.89

0.064

3.2

4.47

0.016

9.87

0.255

6

11L

5.51

0.01

13.64

0.042

3.3

4.36

0.005

9.71

0.066

6.3

5F6L

4.57

0.013

11.48

0.03

4.5

3.76

0.009

7.61

0.043

9.4

5L6R

5.85

0.011

14.1

0.211

3.1

4.51

0.018

10.3

0.123

5.7

5H6R

2.93

0.010

6.68

0.02

11

2.71

0.012

4.92

0.032

19

5H6L

2.62

0.002

5.44

0.001

16

2.48

0.002

3.72

0.04

36

9H2R

2.43

0.005

4.07

0.005

26

2.38

0.016

3.08

0.025

60

IP of the 20 M G6P (DIW, control) after 120 h at the room temperature was changed from 1.67 to 1.83 M. The corresponding half-life was 10450 h. The IP of the 20M ATP (DIW, control) after 120 h at the room temperature was changed from 1.89 to 2.06 M. The corresponding half-life was 9290h.

*The

Table 4 Comparison of catalytic parameters of various inorganic peroxidase TMB Catalyst

Km Vm (mM) (10-8 Ms-1) 0.434 10 HRP (40oC, pH3.5) o 0.098 3.4 Fe3O4 (40 C, pH3.5) Cluster spheres, {311} 0.23 Fe3O4 0.46 (40 °C, pH4.0) Triangular plates, {220} Octahedra , {111} 0.58 Hexagonal prism,110 nm 0.768 0.5 -Fe2O3 Cube-like, 100 nm 0.957 0.63 (37 °C, pH4.0) Rod-1, 30x380 nm 0.257 1.65 Rod-2, 30x330 nm 0.317 1.53 Rod-3, 30x280 nm 0.518 1.06 o 0.67 2.09 MgFe2O4 (45 C, pH4.0) o 0.55 4.57 NiFe2O4 (45 C, pH4.0) 0.85 13.31 ZnFe2O4 (40oC, pH4.0) 0.099 6.6 BM-Fe3O4 (30oC, pH3.8) o 0.009 4.22 FeSe (30 C, pH4.0) 0.008 8.7 FeS (30oC, pH4.0) o 0.548 Fe7S8 (30 C, pH 4.0) 0.525 5.16 Mo2S (30oC, pH6.9). o 2.2 39 CuZnFeS (32 C, pH4.2). 0.007 8.96 CuS(40oC, pH4.0) 0.01 3.57 CdS(40oC, pH4.0) o 1.83 4.31 WS2 (60 C, pH6.9). 3956 376.5 HRP (20oC, pH4.5) Naked 101 432.7 Fe3O4(20oC, pH4.5) Histidine 598 36.2 Alanine 676 83.1 Phenylalanine 608 39.1 Tyroptophan 146 50.2 Methionine 1000 52.5 o {112} 0.09 9.91 Co3O4(25 C, pH6.0) {110} 0.22 7.12 {100} 0.26 6.04 0.018 2.4 NiO (20oC, pH4.0) o 0.023 1.4 Ni(OH) (20 C, pH4.0) o 0.146 131 V2O (20 C, pH5.0) 0.035 73.4 MnSe (20oC, pH5.0) o 0.16 1.15 Fe3S4 (20 C, pH 6.8)

H2O2 Vm Km (mM) (10-8Ms1 ) 3.7 8.71 154 9.78

Reference Gao et.al., 2007 Liu et.al., 2011

Chaudhari et.al. , 2012

4.61 2.6 1.66 50 8.09 9.36 0.9 0.01 0.07 12 3.62 0.24 10.35 458.9 38 226.6 1198 2951 2528 284 455 480 1.77 1.76 1.69 87 1.16

13.46 14.11 7.74 50 6.51 19.2 4.29 0.56 21.9 5.6 4.52 0.069 0.306 0.528 0.445 0.241 0.623 0.468 48.08 39.6 35.81 1.2 1.4 177 4.78 2.17

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