Jan 24, 2006 - work station (Concept 400, Ruskin Technologies, Leeds, UK; Jouan, Saint ...... Chase, A. J., Bond, M., Crook, M. F., Newby, A. C. (2002) Role of ...
Uncorrected Version. Published on January 24, 2006 as DOI:10.1189/jlb.0605302
Hypoxia reduces the output of matrix metalloproteinase-9 (MMP-9) in monocytes by inhibiting its secretion and elevating membranal association Michal A. Rahat,*,1 Barak Marom,*,† Haim Bitterman,† Lea Weiss-Cerem,† Amalia Kinarty,* and Nitza Lahat* *Immunology Research Unit and †Ischemia-Shock Research Laboratory, Carmel Medical Center, Rappaport Family Institute for Research in the Medical Sciences, and Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel
Abstract: Cellular hypoxia, characterizing tumors, ischemia, and inflammation induce recruitment of monocytes/macrophages, immobilize them at the hypoxic site, and alter their function. To migrate across the extracellular matrix and as part of their inflammatory functions, monocytes and macrophages secrete proteases, including matrix metalloproteinase-9 (MMP-9), whose expression is induced by proinflammatory cytokines [e.g., tumor necrosis factor ␣ (TNF-␣)]. We show that hypoxia (
