Sep 17, 2008 - Abstract: Salmonella enterica serovar Typhi is a human pathogenic microorganism with a very complex infective cycle, involving the transit of ...
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The Open Infectious Diseases Journal, 2008, 2, 32-38
Open Access
Identification of Salmonella enterica Serovar Typhi DNA Fragments with Transcriptional Activity Under Different Growth Conditions Daniele Saverino*,1, Jennifer McDermott2, Davide Ferrari3, Marta Terrile4,5 and Gabriella Piatti3 1
Università Degli Studi di Genova, Dipartimento di Medicina Sperimentale, DiMeS, Sezione di Anatomia Umana, Italy
2
Advanced Biotechnology Center, ABC, Genova, Italy
3
Università degli Studi di Genova, Dipartimento Interdisciplinare di Scienze Speialistiche, Chirurgiche, di Microbiologia e dei Trapianti d’Organo (DISCMIT), Sezione di Microbiologia “C. A. Romanzi”, Italy 4
Laboratorio di Oncologia Sperimentale F, Istituto Nazionale per la Ricerca sul Cancro, IST, Genova, Italy
5
Università degli Studi di Genova, Dipartimento di Oncologia, Biologia e Genetica, DOBiG, Italy Abstract: Salmonella enterica serovar Typhi is a human pathogenic microorganism with a very complex infective cycle, involving the transit of bacteria across different microenvironments; to optimize the performance of attenuated Salmonella strains suitable as live carriers of heterologous antigens, fine tuning of wild type bacteria gene expression is essential. Several DNA fragments were obtained from a Salmonella enterica serovar Typhi (vi+, fim+) blood isolate and 18 clones were selected according to the dimension of the insert (range
