IHC Products & Protocol Guide

R&D Systems Tools for Cell Biology Research™

IHC Products & Protocol Guide

IHC & ICC cover images using R&D Systems primary antibodies. Delta-like 1 (DLL1) in Embryonic Rat Stomach

Wnt-2 in Human Stomach Cancer

Tie-2 in Embryonic Zebrafish

Catalog # AF3970

Catalog # AF3464

Catalog # AF928

Vanilloid R1 in Rat Dorsal Root Ganglion

Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC)

IL-2 Receptor a in Mouse Spleen

Catalog # AF3066

Catalog # AF2418/MAB1326

Catalog # AF2438

Notch-2 in Rat Choroid Plexis

STAT6 in Human Peripheral Blood Mononuclear Cells (PBMCs)

Glutathione Peroxidase 1 (GPX1) in Rat Liver

Catalog # AF1190

Catalog # MAB2167

Catalog # AF3798

MESDC2 in Adult Mouse Testis

Meprin b Subunit in Mouse Intestine

GFAP in Rat Astrocytes

Catalog # AF4545

Catalog # AF3300

Catalog # AF2594

To view our continuously updated IHC/ICC image gallery, please visit our website at www.RnDSystems.com/go/IHCimages

TABLE OF CONTENTS This guide provides an introduction to the techniques, protocols, and troubleshooting methods that lead to a successful immunohistochemistry (IHC) or immunocytochemistry (ICC) experiment. Contributors include scientists from R&D Systems IHC/ICC core facility who have experience qualifying thousands of antibodies for these applications, and R&D Systems expert technical service team who have guided researchers through a range of IHC/ICC-related issues.

INTRODUCTION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10

IHC/ICC PROTOCOLS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-20

Designing a Successful IHC/ICC Experiment. . . . . . . . . . . . . . . . . . . . . . . . . . . 2

A. Making a 4% Formaldehyde Solution in PBS . . . . . . . . . . . . . . . . . . . . . 12

Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Paraffin-embedded & Frozen Tissues Cell Samples

B. Preparation of Gelatin-coated Slides for Histological Tissue Sections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Fixation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Formaldehyde, Alcohols, & Acetone Fixation of Tissues Fixation of Cultured Cells Preventing Non-specific Staining. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Preventing Non-specific Hydrophobic Interactions Preventing Non-specific Ionic Interactions Endogenous Enzyme Interference Endogenous Biotin Interference Primary Antibody Selection & Optimization. . . . . . . . . . . . . . . . . . . . . . . . . . 6 Selecting a Primary Antibody Supplier Selecting a Monoclonal or Polyclonal Antibody Affinity Purification of Polyclonal Antibodies Optimization of Primary Antibody Incubation Detection & Visualization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-8 Direct or Indirect Detection, & Amplification Fluorescence Detection Chromogenic Detection

C. Chromogenic Staining of Tissue Sections Using HRP-DAB Detection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14-15 Slide Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Chromogenic Staining Using HRP-DAB Detection . . . . . . . . . . . . . . . . . 15 D. IHC Staining of Tissue Sections Using Fluorescence Microscopy. . . . . . 16 E. Antigen Retrieval Using Heat-induced Epitope Retrieval (HIER) . . . . . 17 F. Preparation of Coverslips & Cell Fixation for ICC. . . . . . . . . . . . . . . . . . . 18 G. Fluorescent ICC Staining of Cultured Cells. . . . . . . . . . . . . . . . . . . . . . . . 19 H. Preparation of a Cell Smear for Non-adherent Cell ICC. . . . . . . . . . . . . . 20 TROUBLESHOOTING GUIDE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 ALPHABETICAL LISTING OF R&D SYSTEMS IHC/ICC ANTIBODIES. . . . . . . . . . . . . . . . . . . . . . . . 22-108 SUPPLEMENTAL IHC/ICC REAGENTS. . . . . . . . . . . . . . . . . . . . . . . . . . 109-112

IHC/ICC Controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Endogenous Tissue Background Control No Primary Antibody Control Isotype Control Absorption Control Tissue Type Control Limitations of Western Blot Comparisons Antigen Retrieval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 Antigen Retrieval Techniques Protease-induced Epitope Retrieval (PIER) Heat-induced Epitope Retrieval (HIER) Optimization & Limitations

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INTRODUCTION Designing a Successful IHC/ICC Experiment Immunohistochemistry (IHC) and immunocytochemistry (ICC) are techniques employed to localize antigen expression and are dependent on specific epitopeantibody interactions. IHC refers to the use of tissue sections, whereas ICC describes the use of cultured cells or cell suspensions. In both methods, positive staining is visualized using a molecular label, which can be fluorescent or chromogenic. Briefly, samples are fixed to preserve cellular integrity, and then subjected to incubation with blocking reagents to prevent non-specific binding of the antibodies. Samples are subsequently incubated with primary and secondary antibodies, and the signal is visualized for microscopic analysis. Technically, IHC/ICC are relatively simple and straightforward experimental methods. However, there are many variables which must be recognized and optimized for each individual IHC/ICC study. The most challenging aspect of these techniques is determining the experimental conditions necessary to generate a strong and specific signal for each antigen of interest. For example, detection of an abundant protein in cultured cells may require a short fixation period, minimal blocking, and may be compatible with direct visualization using a flurochrome-conjugated primary antibody. In contrast, detection of a phosphorylation-dependent epitope in a section of frozen tissue may require antigen retrieval and be dependent on amplified chromogenic visualization. This guide provides information on the IHC/ICC variables listed below and is intended to assist in the design, optimization, and troubleshooting of IHC/ICC experiments.

VARIABLES FOR EXPERIMENTAL DESIGN & OPTIMIZATION VARIABLE

FACTORS

Antigen

Species, expression levels, sample types, & subcellular location

Epitope

Dependence on conformation or post-translation modification

Sample Type

Tissue or cells

Sample Preparation

Cells: adhered cell cultures or cell suspensions No primary antibody, isotype control, absorption control, tissue type control

Fixation Method

Perfusion or immersion (with or without freezing)

Fixative*

Formaldehyde, alcohols, or acetone

Blocking Reagent*

Normal serum, bovine serum albumin (BSA), or non-fat milk

Antigen Retrieval*

Protease-induced Epitope Retrieval (PIER) or Heat-induced Epitope Retrieval (HIER)

Detection Method

Direct or indirect (with or without amplification)

Primary Antibody*

Monoclonal or polyclonal

Secondary Antibody*

Species and label

Labeling Method

Fluorescence or chromogenic

Counterstain* Mounting Reagent Visualization & Analysis

Examples of Fluorescence Detection by IHC & ICC. A. Fluorescence detection by IHC was used to visualize V-set and Ig domain-containing protein 1 (VSIG1) in a frozen section of human testis using anti-human VSIG1 affinity-purified polyclonal antibody (Catalog # AF4818). Tissue was stained using NorthernLights-557 anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue). B. Fluorescence detection was used to measure Glial Fibrillary Acidic Protein (GFAP) expression in differentiating cultures of rat cortical stem cells using anti-human GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The cells were stained using anti-sheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

A.

Fluorochromes and spectral properties

B.

Chromogens: 3,3' Diaminobenzidine (DAB), 3-amino-9-ethylcarbazole (AEC) Fluorescence: 4,6'-diamidino-2-phenylindole (DAPI) Chromogenic: hematoxylin Fluorescence: anti-fade mounting medium Chromogenic: aqueous mounting medium Fluorescence microscope or standard light microscope

* These variables require optimization of additional factors including concentration, pH, temperature, incubation time, and diluent. This table is not intended to be comprehensive but represents the most commonly applied IHC/ICC methods at R&D Systems.

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B.

Tissue: embedded or frozen

Appropriate Controls

Label

A.

Examples of Chromogenic Detection by IHC & ICC. A. Chromogenic detection of Interferon Regulator Transcription Factor 8 (IRF8) expression in paraffin-embedded human lymphoma tissue section using anti-human IRF8 affinity-purified polyclonal antibody (Catalog # AF5117). The tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue). B. Chromogenic detection of IFN-g expression in human peripheral blood mononuclear cells, stimulated with calcium ionomycin (0.5 mg/mL) and PMA (50 ng/mL), using anti-human IFN-g affinity-purified polyclonal antibody (Catalog # AF285). The cells were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

For research use only. Not for use in diagnostic procedures.

INTRODUCTION Sample Preparation Tissue and cell samples must be appropriately harvested and prepared for each IHC/ICC study. To facilitate the required incubation steps, whole tissues must be cut into ultra thin (5-10 mm) sections or cut into smaller pieces for whole mount IHC. For ICC experiments, cells must be attached to a microscope slide or coverslip before commencing the staining procedure. Sample preparation is also intimately linked to the method of fixation, which in turn is influenced by the desired detection technique (fluorescence versus chromogenic). In most circumstances, one experimental variable will determine the most appropriate method of sample preparation. For example, tissue that is immersion-fixed in formaldehyde must be paraffin-embedded and cut using a microtome. Alternatively, to detect a phosphorylationdependent epitope, tissue may need to be snap frozen, which requires a cryostat for sample sectioning followed by alcohol fixation.

Paraffin-embedded Tissue

IHC SAMPLE PREPARATION COMPARISON

Paraffin embedding offers the best option for long-term preservation of tissue samples. Tissue must be fixed before being embedded in paraffin. Fixation can be achieved by perfusion or immersion immediately following dissection, and typically requires 4-24 hours. It is not recommended to fix tissue for more than 24 hours because overfixation can cause masking of the antigen (see page 10). If necessary, tissues can be transferred to alcohol after fixation until there is time for the embedding process. Because paraffin is immiscible with water, tissue must be dehydrated be fore adding molten paraffin wax. Dehydration is achieved by immersion in increasing concentrations of alcohol. This approach allows for a gradual change in hydropho bicity and minimizes cell damage. Following dehydration, the tissue is incubated with xylene to clear any remaining ethanol. Paraffin is typi cally heated to 60 ˚C for embedding and is subsequently allowed to harden overnight. The tissue is subsequently cut with a sharp blade into ultra thin slices using a microtome. Sections are then dried onto microscope slides and can be stored at room temperature for extended periods of time. Tissue sections must be rehydrated before commencing the IHC/ICC protocol (see page 14). Paraffin is the cheapest and most commonly used substance for tissue embedding. However, tissue can also be embedded in plastic, which sets harder and allows thinner tissue sections (1.5 mm versus 5 mm).

Frozen Tissue One of the benefits of frozen tissue samples is the time saved by omitting the initial fixation step required for paraffin-embedded tissue. Snap freezing can be particularly beneficial when detecting post-translational modifications such as phosphorylation. Frozen tissue can be prepared by immersing the tissue in liquid nitrogen or isopentane, or by burying it in dry ice. For frozen samples, a short fixation is done after freezing and sectioning. Frozen tissue sections are most often fixed using alcohol, which avoids the possible requirement to retrieve epitopes masked by formaldehyde cross-linking. Frozen tissue is cut on a cryostat and sections can be stored at - 80 ˚C for up to 1 year.

TISSUE FIXATION

Paraffin-embedded

Frozen

Pre-embedding

Post-sectioning

Microtome

Cryostat

Multiple years at room temperature

1 year at - 80 ˚C

ADVANTAGES

Preserves tissue morphology

Preserves enzyme and antigen function

LIMITATIONS

Overfixation can mask the epitope

Formation of ice crystals may negatively affect tissue structure

SECTIONING STORAGE

Cell Samples To perform ICC studies, the cells must first be attached to a solid support, such as a microscope slide or coverslip. This is simple with adherent cells, which can be grown directly on high-grade, sterilized coverslips placed on the bottom of a 6- or 24-well plate. Cell adhesion can be enhanced by precoating the coverslip with a charged polymer like Poly-L-Lysine and/or an extracellular matrix protein such as Laminin, Fibronectin, or Collagen. All steps of the staining process can be performed with the coverslip in the microplate by adding the appropriate solution to each well and removing solutions by aspiration. Cells in suspension can be adhered to a slide by Cytospin® centrifugation or by a 10 minute incubation of the cells on slides that have been coated with Poly-L-Lysine. Note: It is important to be able to recognize artifacts of poorly prepared samples in order to properly interpret results. For example, folds or tears in the tissue, or bubbles between the tissue/cells and the slide, can collect staining reagents non-specifically and obscure positive staining or lead to false-positive signals.

Frozen tissue sections can be processed in a shorter amount of time than paraffin-embedded sections. However, freezing is not adequate for longterm preservation of the tissues and the formation of ice crystals within the cells may negatively affect subcellular detail. In addition, frozen sections are often thicker than paraffin sections. This can increase the potential for lower microscopic resolution and poor images of tissue morphology. Because frozen tissue often retains enzymatic activities, blocking the functions of endogenous enzymes that may affect the IHC detection method is also particularly important (see page 5).

Cytospin is a registered trademark of Thermo Electron Corporation.


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INTRODUCTION Fixation All samples used in IHC/ICC experiments must be fixed to preserve tissue morphology and retain the antigenicity of the target molecules. Fixation alters the chemical composition of tissues and often requires a compromise between preserving tissue structure and preserving the epitope. Incomplete fixation (underfixation) of cells or tissues may allow rapid proteolytic degradation of target proteins within the tissue and can reduce specific immunoreactivity. However, excessive fixation (overfixation) may result in masking of the epitope or in strong non-specific background staining that can obscure specific labeling. As discussed in the previous section, the method and timing of fixation must be considered in terms of sample preparation. In addition, duration, temperature, and pH will all affect the degree of fixation.

Formaldehyde

Acetone

Formaldehyde is the most common fixative used to preserve protein targets within tissues and cells. Formaldehyde-mediated tissue fixation is thought to be dependent on the formation of protein-protein and proteinnucleic acid cross-links involving methylene bridges (-CH2-). Formaldehyde can chemically link NH2 (amino) and CONH (peptide) groups, NH2 and NH, or NH2 and NH2 groups.

Acetone is a strong dehydrating agent, which can cause irreversible pre cipitation of tissue proteins. It is usually used on sections of unfixed, snapfrozen tissues, followed by fixation with alcohols or formaldehyde.

Formaldehyde is a good choice for most IHC/ICC applications but is not a universal fixative. Overfixation with formaldehyde can modify the amino acids that are part of an epitope and can block an antibody from binding to it. However, in most cases the epitope can be unmasked to restore antibody bind ing using antigen retrieval techniques (see page 10). Formaldehyde has been shown to induce intracellular translocation of phosphorylation-dependent epitopes from the membrane to the cytoplasm. In situations like this, ice cold absolute methanol or absolute ethanol are appropriate alternative fixatives. Note: Although there are many different fixatives available, formaldehyde is the most commonly used, and is an appropriate starting point for most IHC/ICC experiments. Formaldehyde-based solutions should be aliquoted and frozen, or stored at 4-8 ˚C for no longer than one month. See page 12 for a protocol for making a typical 4% formaldehyde solution.

Alcohols The most popular alcohols used for cell and tissue fixation are methanol and ethanol. The molecular structures of methanol and ethanol closely resemble that of water. Therefore, they compete with water for protein hydrogen bonds, replacing water molecules in tissues. This causes pro teins to precipitate at their isoelectric points by reducing their dielectric constant, and can block antibody-epitope binding due to changes in conformation. While alcohols affect the tertiary structure of proteins by breaking hydrophobic bonds, it appears that they can stabilize the secondary structure of proteins. However, it is generally thought that alcohols do not preserve tissue morphology as well as formaldehyde-based fixatives. Alcohols do not penetrate as well as formaldehyde and are primarily used to fix frozen tissue sections and cells. Consequently, alcohol fixation is more suitable for membrane surface antigens. Antigen retrieval is not recommended following alcohol fixation as it is generally considered too harsh and will likely compromise the integrity of the tissue sections or cells.

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Fixation of Tissues Whole animal perfusion fixation is often the best way to preserve antigens when studying intact tissues of small animals such as mice, rats, and guinea pigs. It involves replacing the animal’s systemic blood with a fixative solution. However, whole animal perfusion may not be sufficient to fix the tissues of interest. In cases such as these, dissected tissues can be immersed in the fixative. For example, 4% formaldehyde in PBS is a commonly used solution for perfusion and immersion fixation of tissues. To enhance penetration of the fixative during immersion fixation, it is recommended that tissues be no thicker than 10 mm. For complete fixation, the volume of the fixative should be 50-100 times larger than the volume of the tissue. Fixation is usually achieved at room temperature for 4-24 hours. It is very important to optimize fixation conditions since underfixation or overfixation may reduce or abolish tissue immunoreactivity.

Fixation of Cultured Cells In contrast to tissue samples, cultured cells can be fixed for a shorter period of time with a less concentrated fixative solution. For example, fix ation with a 2% formaldehyde solution for 20 minutes at room tempera ture is often sufficient to preserve both cellular morphology and antigenicity. Fixation of cultured cells is usually achieved by simply discarding the culture medium and adding the fixative solution. However, the change in surface tension following removal of the culture medium can damage some cell types. If this is the case, the fixative can be added directly into the culture medium. For example, adding the same volume of 4% form aldehyde as the volume of culture medium will result in a 2% formalde hyde solution, which is strong enough to pre-fix the cells. After 2 minutes, the pre-fixation culture medium should be replaced with a fresh volume of 2% fixative. The pre-fixation step makes the cells more rigid so they can withstand any potential deleterious effects created by changes in surface tension. Note: Fixation can result in hydrophobic cross-linking of tissue proteins. The time, temperature, pH, and fixative used will determine the degree of cross-linking. Once the fixation protocol has been optimized, the same procedure should be used consistently.


INTRODUCTION Preventing Non-specific Staining Once the tissue or cell samples have been appropriately prepared and fixed, the samples are ready to be stained. All IHC/ICC studies are dependent on specific antibody-epitope binding, which is governed by hydrophobic interactions, ionic interactions, hydrogen bonding, and other intermolecular forces. However, the same attractive forces can also result in non-specific staining, i.e. binding of the primary antibody to amino acids other than those within the desired epitope of the antigen. This is a common problem that occurs in IHC/ICC experiments. The challenge is to reduce non-specific interactions without impairing antibody-epitope binding. Causes of non-specific staining include interactions of the primary and secondary antibodies with serum proteins, ionic interactions between antibodies and tissues, and interactions with endogenous molecules capable of affecting the IHC detection system used. These issues can result in high background and an inability to visualize the antigen of interest in its appropriate cellular location. Staining problems of this type can be addressed by blocking non-specific interactions using a blocking reagent. These steps are performed prior to incubation of the sample with the primary antibody.

Preventing Non-specific Hydrophobic Interactions Although hydrophobic interactions play an essential role in epitopeantibody binding, these forces can also promote non-specific binding. Most proteins have some degree of hydrophobicity due to the neutral side chains of several amino acids. Tissue incubation with heat-inactivated normal serum or bovine serum albumin (BSA) is a common procedure used to reduce non-specific hydrophobic binding. Selection of the type of normal serum is important to prevent interactions with the primary or secondary antibodies, or with the tissues/cells being stained. For example, goat serum would not be advised as a blocking reagent for use with a goat-derived primary antibody. Instead, a serum identical to the host animal of the secondary antibody or from an unrelated species is recommended. BSA and non-fat dry milk are also frequently used as blocking reagents. One of these reagents is typically included in the diluents for the primary and secondary antibodies. The addition of non-ionic detergents including 0.3% Triton X-100™ or Tween 20™ can also reduce nonspecific hydrophobic interactions.

Chromogenic detection methods often use a conjugated enzyme to vis ualize epitope-antibody interactions. When using this method of detection, the endogenous activity of the same enzyme must be blocked. For example, protocols that include horseradish peroxidase (HRP) or alkaline phospha tase (AP) may require reagents to prevent non-specific signals. Tissues such as kidney, liver, or vascular areas with red blood cells, contain endogenous peroxidase activity. Peroxidase blocking reagents formulated with 3-10% H2O2 can be used to prevent endogenous peroxidase from cleaving the substrate. Endogenous AP found in intestine, kidney, lymphoid and other tissue can be blocked with 1 mM Levamisole. The intestinal form of AP is unaffected by Levamisole but can be blocked by using 1% acetic acid.

A.

A.

B.

Blocking Non-specific Binding with Serum. A. CD14 was detected in paraffin-embedded human tonsil tissue using anti-human CD14 biotinylated affinity-purified polyclonal antibody (Catalog # BAF383). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using high sensitivity streptavidin conjugated to HRP (HSS-HRP) and DAB, and counterstained with hematoxylin (blue). B. Non-specific background staining is markedly reduced in a parallel experiment which included a blocking step using animal serum for 15 minutes at room temperature prior to incubation with the primary antibody. HSS-HRP and animal blocking serums are included in all R&D Systems Cell and Tissue Staining Kits (see page 112 for available kits).

Preventing Non-specific Ionic Interactions If the antibody employed and the target tissue have net opposite charge, ionic interactions can cause non-specific background staining. For ex ample, non-specific staining may be due to attraction between opposing carboxyl and amino groups. Van der Waals forces, weak electrostatic interactions between dipolar molecules, can also contribute. Increasing the ionic strength of the fixative and/or the antibody diluent buffer can reduce ionic interactions. However, epitope-antibody binding is common ly dependent on ionic forces, so this approach also has the potential to

negatively impact staining specificity. Due to their single epitope speci ficity, increasing ionic strength is more likely to impair monoclonal than polyclonal antibody performance.

Endogenous Enzyme Interference

B.

Quenching Endogenous Peroxidase Activity. A. Failing to quench endogenous peroxidase prior to staining produced a false positive signal in sections of human kidney. Tissue was stained using the anti-goat HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS008; brown). B. Endogenous peroxidase activity was quenched in the same tissue using 3% H2O2 in methanol for 15 minutes at room temperature prior to staining.

Endogenous Biotin Interference Streptavidin binding to a biotinylated primary or secondary antibody is another common detection system that is utilized for IHC/ICC experiments (see page 7). Therefore endogenous biotin must be blocked before strepta vidin incubation. Endogenous biotin is found in many tissues including liver, kidney, heart, brain, and lung. Pre-incubation of the sample with avidin is routinely used to block endogenous biotin. This must be followed by a sub sequent incubation with biotin to block additional biotin binding sites on the avidin molecule. Following these biotin/avidin blocking steps, the sample can be incubated with the primary antibody.

Triton X-100 is a trademark of Dow Chemical Corporation. Tween is a trademark of ICI Americas.


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INTRODUCTION Primary Antibody Selection & Optimization The most important factor when designing an IHC/ICC experiment is selection of the primary antibody. In turn, the critical feature of a primary antibody is specificity for the epitope. All steps of an IHC/ICC experiment must be optimized to visualize specific staining and minimize non-specific background signals. This includes performing initial studies to determine the appropriate incubation conditions for each primary antibody. The working dilution for an antigen affinity-purified polyclonal antibody is generally lower than that of a monoclonal antibody but these values must be determined empirically. To achieve a robust and specific signal, a high quality antibody that exhibits minimal cross-reactivity should be employed.

Selecting a Primary Antibody Supplier When searching for a primary antibody, it is likely that products will be available from several different commercial sources that may offer varying degrees of antibody quality. This initial choice can make the difference between a successful experiment and a missed opportunity. A first step is to look for independent verification. This includes checking for prior successful use of the antibody in the literature. Many commercial suppliers, including R&D Systems, can provide assistance to researchers looking for this information. Another potential question to ask is whether the antibody is actually manufactured by the supplier or is it purchased and resold? Purchasing direct ensures the commercial source has had complete control of manufacturing, quality control processes, and shipping and storage conditions. In addition, the manufacturer may be in the best position to provide quality technical service should questions arise about the use of the antibody.

Selecting a Monoclonal or Polyclonal Antibody The inherent features of monoclonal and polyclonal antibodies are as sociated with advantages and limitations when applied to IHC/ICC. Mono clonal antibodies, produced from a single B cell clone, represent a homo geneous population that bind with high affinity and specificity to a single epitope. This can be particularly useful when detecting a single member of a protein family that shares a high percentage of amino acid identity. Antibody binding is often dependent on the target protein maintaining its native conformational state. Access to a desired epitope can be com promised by interactions with other proteins, post-translational modifica tions, temperature, pH, fixation, and salt concentration. Because polyclonal antibodies are heterogeneous and can recognize multiple epitopes, they are less likely to be affected by changes in protein conformation. In general, polyclonal antibodies are also more stable than monoclonal antibodies over a range of pH and salt concentration. For these reasons, polyclonal anti bodies are more frequently used for IHC/ICC experiments than monoclonal antibodies.

Affinity Purification of Polyclonal Antibodies Polyclonal antiserum contains a mixture of antibodies produced by a large number of B cell clones. Antibodies comprising polyclonal antiserum bind to target epitopes with different specificities and affinities, as well as crossreact with irrelevant target molecules (non-specific interactions). To enrich for antibodies with the highest specificity and affinity toward the antigen of interest, R&D Systems polyclonal antibodies are antigen affinity purified. During this process polyclonal antiserum is passed through an affinity column containing immobilized antigen molecules. Specific antibodies are retained by the immobilized antigen, whereas non-specific antibodies pass through the column and are discarded. The retained antigen affinity purified antibodies are subsequently eluted from the column. Antigen af finity purified antibodies interact predominantly with their target antigen,

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reduce background staining, and produce more consistent results com pared to non-purified antibodies. Monoclonal Antibody Binding

Polyclonal Antibody Binding

Monoclonal and Polyclonal Antibody Binding. Monoclonal antibodies bind only a single epitope, while polyclonal antibodies bind different epitopes on the same protein,

Optimization of Primary Antibody Incubation Primary antibody concentration, diluent, incubation time, and temperature all impact the quality of staining. These variables need to be optimized for each antibody and sample to achieve specific staining and low background. Often optimization is approached by maintaining a constant incubation time and temperature, while varying the antibody concentration to deter mine when an optimal signal is achieved with low background noise. For example, if a high affinity antibody is used, then the antibody can be used at a relatively high concentration for a shorter incubation time. Alternatively, a high affinity antibody at lower antibody concentration may be used with a longer incubation time. Longer incubation durations are often employed to ensure penetration throughout tissue sections used for stereological techniques. To promote specific staining, longer incubation periods are often conducted at lower temperatures (i.e. 4 ˚C versus room temperature). Optimization of R&D Systems staining protocols for tissue sections typi cally begin with an overnight incubation with the primary antibody at 4 ˚C. For staining cells, a 1 hour incubation with the primary antibody at room temperature is a common starting point. The working dilution of an anti gen affinity purified polyclonal antibody is generally lower (from 1.7-15 mg/mL) than that of a monoclonal antibody (5-25 mg/mL). When comparing samples stained with different concentrations of the same antibody, it is important to use a consistent time and temperature for the incubation step. When working with a new antibody for the first time, it is recom mended to conduct preliminary studies testing a broad range of antibody concentrations.

STARTING CONDITIONS FOR PRIMARY ANTIBODY INCUBATION ANTIBODY

SAMPLE

Monoclonal

Polyclonal

Tissue

5-25 mg/mL, overnight at 4 ˚C

1.7-15 mg/mL, overnight at 4 ˚C

Cells

5-25 mg/mL, 1 hour at room temperature

1.7-15 mg/mL, 1 hour at room temperature

Advantage

Single epitope specificity

Lower concentration required

Limitation

Vulnerable to epitope masking

Heterogeneous population


INTRODUCTION Detection & Visualization Following incubation with the primary antibody, antibody binding is visualized using an appropriate detection system. The method of detection can be direct or indirect, and may generate a fluorescent or chromogenic signal. Direct detection involves the use of primary antibodies that are directly conjugated to a label. Indirect detection methods utilize a labeled secondary antibody raised against the primary antibody host species. Indirect methods can also include amplification steps to increase signal intensity. Commonly used labels for the visualization of epitope-antibody interactions include fluorophores and enzymes that convert soluble substrates into insoluble, chromogenic end products. The choice of label is affected by the detection method employed, personal preference, and the type microscope equipment that is available

Direct or Indirect Detection, & Amplification

DETECTION METHODS & SIGNAL INTENSITY

The choice of whether to use direct or indirect detection is often dictated by the level of antigen expression. For example, detection of a highly expressed epitope might be possible using a primary antibody directly conjugated to a label. Advantages of direct detection include the ease of use for multicolor staining and the elimination of concerns regarding nonspecific binding of the secondary antibody. The major disadvantage is that direct detection may lack the sensitivity required to visualize lower expression levels. In rare cases the conjugation process may also have a negative impact on the affinity of the primary antibody.

DIRECT

ADVANTAGE

LIMITATION

Good for multicolor labeling

Lower sensitivity

In contrast, indirect detection methods generally have a higher level of sensitivity and generate a more intense signal. The signal is amplified using the indirect method because of the potential for at least two labeled secondary antibodies to bind to each primary antibody. However, the use of a secondary antibody requires extra blocking steps and additional controls. Further amplification of the signal can be achieved by taking advantage of the strong affinity of avidin and streptavidin to bind biotin. Avidin is a glycoprotein in egg white that combines stoichiometrically with biotin. Streptavidin is purified from the bacterium Streptomyces avidinii, is not glycosylated, and exhibits lower non-specific binding than avidin. Both proteins bind four biotins per molecule. If a biotinylated secondary anti body is employed, the signal can be significantly amplified by subsequent incubation with an avidin-biotin complex (ABC Method), or labeled streptavidin-biotin (LSAB Method). Streptavidin may be conjugated to a detection enzyme (i.e. horseradish peroxidase or alkaline phosphatase) or fluorochrome. Using these amplification methods requires additional steps to prevent non-specific binding (see page 5).

KEY:

INDIRECT

ADVANTAGE

LIMITATION

Higher sensitivity

Potential non-specific binding of secondary antibodies AMPLIFICATION – ABC METHOD

ADVANTAGE

LIMITATION

Increased signal intensity

Requires additional time & controls

Antigen AMPLIFICATION – LSAB METHOD

Primary Antibody Secondary Antibody Label Avidin/Streptavidin Biotin

ADVANTAGE

LIMITATION

Increased signal intensity

Requires additional time & controls


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INTRODUCTION Fluorescence Detection

Multicolor experiments must be designed to limit cross-reactivity between the detection reagents and crossover between the spectral properties of the fluorochromes being used. To limit cross-reactivity between secondary reagents, the primary antibodies should be derived from different species. This allows for the use of species-specific secondary antibodies that recognize only one primary antibody. However, there may be some instances when it is necessary to simultaneously use antibodies from the same species. This can be achieved using a biotinylated form of one of the primary antibodies. When using this technique, tissues must be incubated with the non-biotinylated antibody first, followed by incubation with the fluorophore-conjugated secondary antibody. The tissues are then incubated with the biotinylated primary antibody followed by incubation with a streptavidin-conjugated fluorochrome. This approach will ensure that the streptavidin conjugate will only bind the biotinylated antibody, limiting cross-reactivity.

Emission Filter Dichroic Mirror TYPICAL FLUORESCENCE FILTER SET Excitation Filter Sample

Multicolor Fluorescence Microscopy. A typical immunofluorescence setup that depicts the light source, a suitable filter set for the fluorochrome of interest, and a detection mechanism.

NorthernLights is a trademark of R&D Systems.

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400

450

500

550

600

650

700

Wavelength (nm)

B.

R&D Systems NorthernLights™ Fluorochromes have Non-overlapping Spectral Proper ties that make them Ideal for Multicolor IHC and ICC. A. Emission curves for NorthernLights-493 anti-goat IgG (Catalog # NL003; green) and NorthernLights-557 anti-mouse IgG (Catalog # NL007; red). B. Multicolor detection of Nestin and b-III Tubulin in rat cortical stem cells (Catalog # NSC001) using anti-rat Nestin affinity-purified polyclonal antibody (Catalog # AF2736; green) and anti-neuron specific b-III Tubulin monoclonal (clone TuJ-1) antibody (Catalog # MAB1195; red). Cells were stained with NorthernLights-493 (Catalog # NL003; green) and NorthernLights-557 (Catalog # NL007; red) secondary antibodies and counterstained with DAPI (blue).

Detection

Excitation Light Source

A.

Emission Intensity

Fluorescence detection is based on the use of fluorochromes that emit light when excited by light of a shorter wavelength. The fluorochrome can be conjugated directly to the primary or secondary antibody, or to streptavidin. Immunofluorescence is commonly used for the simultaneous visualization of multiple cellular targets. For example, tissues can be incubated with a mixture of different primary antibodies followed by incubation with secondary reagents conjugated to fluorochromes that emit light at different wavelengths.

Chromogenic Detection To facilitate chromogenic detection, the primary antibody, secondary anti body, or streptavidin is conjugated to an enzyme. When a soluble organic substrate is applied, the enzyme reacts with the substrate to generate an insoluble colored product that is localized to the sites of antigen expression. Commonly used enzymes include horseradish peroxidase (HRP) and alkaline phosphatase, which convert 3,3' diaminobenzidine (DAB) and 3-amino-9-ethylcarbazole (AEC), into brown and red end products, re spectively. DAB is more commonly used than AEC since the latter is soluble in alcohol and is more prone to fade when exposed to excessive light. Aqueous counterstain and mounting media must be used with AEC. Chromogenic detection is considered to be more sensitive than that of immunofluorescence, but is less convenient because it includes more incubation and blocking steps. Like immunofluorescence, chromogenic detection allows for the visualization of multiple antigens, but only if the antigens are confined to different locations in the cell and tissue because overlapping colors may obscure results. An advantage of DAB chromogen ic staining is that the colored precipitate formed during the reaction between HRP and DAB is not sensitive to light and the slides can be stored for many years. Unlike fluorescence microscopy, which requires a specialized light source and filter sets, chromogenic techniques require only a typical light microscope.


INTRODUCTION IHC/ICC Controls Appropriate controls are critical for the accurate interpretation of IHC/ICC results. A satisfactory IHC/ICC experimental design produces results that demonstrate that the antigen is localized to the correct specialized tissues, cell types, or subcellular location. Optimization of fixation, blocking, antibody incubation, and antigen retrieval steps will generate a strong and specific signal. However, IHC/ICC experiments must include positive and negative controls to support the validity of staining and identify experimental artifacts. In addition, variances in antibody specificity, experimental conditions, biological conditions between tissue types, and even researchers may generate inconsistent staining and lead to inaccurate conclusions. To facilitate consistent performance, detailed record keeping is an important factor for IHC/ICC studies. Here, we describe several established controls that can be used to support the specificity of your IHC/ICC results.

Endogenous Tissue Background Control Certain cells and tissues may have inherent biological properties resulting in background staining that could lead to a misinterpretation of the re sults. Before applying primary antibodies, cells and tissues should be in spected under the microscope using either fluorescence (for fluorescent labels) or bright-field (for chromogenic labels) illumination to ensure there is no signal inherent to the tissue itself. For instance, lipofuscin is an endogenous autofluorescent pigment that can be confused with positive staining.

A.

B.

C.

The antigen to antibody mixture should be made at a working dilution of 10:1 (molar ratio) and be pre-incubated overnight at 4 ˚C. The pre-absorbed antibody can then be incubated with tissue in place of the primary antibody alone. The staining pattern produced by the primary antibody can be compared to that produced by the pre-absorbed antibody. Absorption controls work better if the immunogens are peptides. However, if antibodies were raised against the whole protein, addition of the mixture of antibody plus protein may result in higher non-specific staining. Although the mechanism is unclear, the antigen used for pre-absorption may itself bind to the tissue and result in non-specific staining. Thus, it is important to note that an absorption control using whole protein may not always confirm the specificity of an antibody for the protein in the tissue.

A.

B.

Lipofuscin Background in Nervous System Tissue. Lipofuscin is a pigment that accumulates with age in many tissue types. It also has autofluorescent properties that overlap with the excitation and emission spectra of commonly used fluorochromes. Circled in the micrographs above are lipofuscin-containing neurons that may appear labeled using either bright-field microscopy (A) or fluorescence microscopy in the green (B) and red spectrums (C).

No Primary Antibody Control A control in which the tissue is incubated with antibody diluent, without the primary antibody included, is always necessary. This is followed by incubation with secondary antibodies and detection reagents. Staining with detection reagents alone should be negligible to the point that it does not obscure specific staining or resemble the specific staining pattern.

Isotype Control This control can be utilized when working with monoclonal primary antibodies. The sample is incubated with antibody diluent, supplemented with a non-immune immunoglobulin of the same isotype (for example, IgG1, IgG2A, IgG2B, IgM) and concentration as the primary monoclonal anti body. The sample is then incubated with the secondary antibody and de tection reagents. These steps will help ensure that what appears to be specific staining was not caused by non-specific interactions of immuno globulin molecules with the sample. Background staining should be neg ligible and not resemble specific staining.

Absorption Control To demonstrate that an antibody is binding specifically to the antigen of interest, it is first pre-incubated with the immunogen. This should inactivate the antibody and the tissue should show little or no staining.

Absorption Control in Rat Dorsal Root Ganglion. A. A cryostat section of rat dorsal root ganglion stained for phospho-MSK1 (S212) using anti-human Phospho-MSK1 (S212) affinity purified polyclonal antibody (Catalog # AF1036). B. Nuclear staining (indicated by arrows) is abolished if the antibody is first pre-absorbed with the S212 phosphorylated immunogen.

Tissue Type Control Additional controls for IHC/ICC experiments include using tissue samples that are known to express (or not express) the epitope of interest. This strategy can provide a useful reference and may also be utilized during initial optimization studies. Tissue from transgenic animals that overexpress or do not express the antigen can be particularly useful. In addition, tissue samples from different species can be included to support the speciesspecificity of an antibody.

Limitations of Western Blot Comparisons Western blot experiments are often conducted to complement and support IHC/ICC studies. However, changes in protein conformation during denaturation can result in potentially misleading results. For a given antibody, inconsistent results between Western blot and IHC/ICC studies may simply reflect differences in the experimental conditions employed. Since multiple epitopes are recognized, polyclonal antibodies are less vulnerable to such experimental artifacts.


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INTRODUCTION Antigen Retrieval Although fixation is essential for the preservation of tissue morphology, this process can also have a negative impact on IHC/ICC detection. Fixation can alter protein biochemistry such that the epitope of interest is masked and can no longer bind to the primary antibody. Masking of the epitope can be caused by cross-linking of amino acids within the epitope, cross-linking unrelated peptides at or near an epitope, altering the conformation of an epitope, or altering the electrostatic charge of the antigen. Antigen retrieval refers to any technique in which the masking of an epitope is reversed and epitope-antibody binding is restored.

Antigen Retrieval Techniques The need for antigen retrieval depends on multiple variables, including but not limited to, the target antigen, the antibody used, the type of tissue, and the method and duration of fixation. Because they recognize multiple epitopes, polyclonal antibodies are more likely than monoclonal antibod ies to detect a given antigen without the use of antigen retrieval techniques. There are multiple techniques to restore the immunoreactivity of an epitope. Methods as simple as changing the pH or the cation concentration of the antibody diluent can influence the affinity of an antibody for its epitope. For partially masked epitopes it may be appropriate to first try increasing the primary antibody incubation conditions before commen cing antigen retrieval. However, this step will also require further op timization in terms of appropriate antibody concentration, incubation time, and temperature. When discussing antigen retrieval methods, tech niques generally fall into two main categories, protease-induced epitope retrieval (PIER) and heat-induced epitope retrieval (HIER). Once optimized, the effects of antigen retrieval can be pronounced.

Protease-induced Epitope Retrieval (PIER) In the PIER method, enzymes including Proteinase K, Trypsin, and Pepsin have been used successfully to restore the binding of an antibody to its epitope. The mechanism of action is thought to be the cleavage of pep tides that may be masking the epitope. The disadvantages of PIER are the low success rate for restoring immunoreactivity and the potential for destroying both tissue morphology and the antigen of interest.

Heat-induced Epitope Retrieval (HIER)

artifactual staining should always be considered when using any antigen retrieval methodology. The use of controls to demonstrate specific anti body binding should be included since staining is influenced by multiple variables in any given experiment. TIME

ANTIGEN RETRIEVAL SOLUTION pH Acidic

Neutral

Basic

1 minute

Slide #1

Slide #2

Slide #3

5 minutes

Slide #4

Slide #5

Slide #6

10 minutes

Slide #7

Slide #8

Slide #9

Optimization of HIER. A typical experimental set-up to determine optimum HIER incubation time and pH. Results should be compared to a tenth slide, with no HIER. A similar approach can be employed to optimize incubation temperature.

R&D Systems offers reagents to improve tissue antigen detection and enhance immunoreactivity. During initial optimization, investigators can compare samples treated with neutral pH 7.0 Universal Antigen Retrieval Solution (Catalog # CTS015) with a slide of the same tissue without antigen retrieval. Subsequent tests using acidic or basic antigen retrieval solutions may be necessary depending on the tissue. At R&D Systems, we find that treatment with Basic Antigen Retrieval reagent (Catalog # CTS013) is frequently successful. We also offer Acidic (Catalog # CTS014) and Sampler (Catalog # CTS016) Antigen Retrieval Reagents. Acidic and basic antigen retrieval solutions are more likely to affect tissue morphology than a neutral solution.

No HIER

Acidic HIER

Neutral HIER

Basic HIER

HIER is believed to reverse some cross-links and allows for restoration of secondary or tertiary structure of the epitope. The protocol must be opti mized for each tissue, fixation method, and antigen to be studied. In gen eral, HIER has a much higher success rate than PIER. HIER is performed using microwave ovens, pressure cookers, vegetable steamers, autoclaves, or water baths. Microwaves are an increasingly pop ular appliance for HIER. These protocols tend to involve 5 minute periods of heat followed by replacement of the buffer. For all HIER methods, slides must be cooled before commencing IHC/ICC incubations. HIER is especially time-, temperature-, buffer-, and pH-sensitive, and the best method must be determined empirically.

Optimization & Limitations Antigen retrieval may require increased adherence of the sample to the slide or coverslip. In addition, the technique is often too harsh for cryostat tissue sections and alcohol-fixed tissue. The time, temperature, and pH must also be optimized for each appliance (i.e. 5-10 minutes at 92-95 °C in a water bath, versus 1-5 minutes at 120 °C in a pressure cooker). To optimize antigen retrieval preliminary studies must be conducted using a matrix of time, temperature, and pH combinations. The possibility of

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Antigen Retrieval Improves Detection of p27. IHC images show the detection of p27 in paraffinembedded human prostate cancer sections following incubation of tissue for 10 minutes at 95 ˚C in the specified antigen retrieval solution. Compared to no HIER treatment, p27 detection was enhanced following incubation in neutral (pH 7.0) and basic (pH 9.5) (Catalog # CTS013) but not acidic (pH 5.0) (Catalog # CTS014) antigen retrieval solution. p27 was detected using antihuman/mouse/rat p27 monoclonal antibody (Catalog # MAB22561; brown).


IHC/ICC PROTOCOLS

IHC/ICC PROTOCOLS Immunohistochemistry

Immunocytochemistry

BLOCK PRIMARY ANTIBODIES SECONDARY ANTIBODIES LABEL COUNTERSTAIN COVERSLIP

RAGE in Alzheimer’s Brain.

Meteorin in Rat Cortical Stem Cells.


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IHC/ICC PROTOCOLS A. Making a 4% Formaldehyde Solution in PBS The vast majority of IHC/ICC procedures employ fixation of tissues and cells using formaldehyde-based fixatives. The protocol below describes the technique for generating a 4% formaldehyde solution in PBS. The most effective fixative must be determined experimentally. Caution: Formaldehyde is toxic, read the MSDS before working with this chemical. Gloves and safety glasses should be worn, and solutions made inside a fume hood. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS  Deionized H2O  HCl (Dilute)  NaOH (1 N)  Paraformaldehyde powder  PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4

40 g Paraformaldehyde Powder 1 N NaOH UNTIL POWDER DISSOLVES

MATERIALS  Filter units  Glassware and stir bar (Dedicated for formaldehyde solution)  Gloves and eye protection  Hot plate with magnetic stirrer  Thermometer  Ventilated hood

800 mL 1x PBS AT ≈ 60 ˚C

HEAT WHILE STIRRING

PROCEDURE 1. For 1 L of 4% Formaldehyde add 800 mL of 1x PBS to a glass beaker on a stir plate in a ventilated hood. Heat while stirring to approximately 60 ˚C. Take care that the solution does not boil. 2. Add 40 g of paraformaldehyde powder to the heated PBS solution.

COOL & FILTER WITH FILTER PAPER

3. The powder will not immediately dissolve into solution. Slowly raise the pH by adding 1 N NaOH dropwise from a pipet until the solution clears.

ADJUST THE VOLUME TO 1 L WITH 1x PBS

4. Once the paraformaldehyde is dissolved the solution should be cooled and filtered. 5. Adjust the volume of the solution to 1 L with 1x PBS. 6. Recheck the pH and adjust it with small amounts of dilute HCl to approximately 6.9. 7. The solution can be aliquoted and frozen, or stored at 4 ˚C for no longer than one month.

H C

O

H Formaldehyde

H2O

H

OH C

H

OH Methylene Glycol

Formaldehyde Structure. In an aqueous solution, formaldehyde exists primarily as methylene glycol.

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ADJUST THE pH TO 6.9 WITH DILUTE HCl ALIQUOT & FREEZE

The difference between paraformaldehyde, formaldehyde, & formalin. Paraformaldehyde (chemical name is polyoxymethylene) is a powder of polymerized formaldehyde that by itself cannot fix tissues. To be usable as a tissue fixative, paraformaldehyde has to be dissolved in hot water to become a formaldehyde solution. Formalin is a saturated formaldehyde solution in water (37% by weight, 40% by volume) containing 10-15% methanol. Methanol is added to slow down the polymerization of formaldehyde, which reduces the fixing power of formalin. Formalin can also be made in an alcohol-free form from powdered paraformaldehyde.


IHC/ICC PROTOCOLS B. Preparation of Gelatin-coated Slides for Histological Tissue Sections In order for tissue sections to be retained on histological slides during staining and washing steps, slides need to be coated with adhesive compounds. Although there are a variety of such compounds, gelatin is the most frequently used for histological purposes. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED

5 g Gelatin AFTER THE GELATIN DISSOLVES ADD 0.5 g CrK(SO4)2

REAGENTS  Gelatin-coating solution: 1 L deionized H2O, 5 g gelatin, 0.5 g chromium potassium sulfate CrK(SO4)2 MATERIALS  Filter units  Histological slides  Hot plate with magnetic stirrer  Slide racks  Staining dish  Thermometer

HEAT AT 45 ˚C WHILE STIRRING

1 L Deionized H20

PROCEDURE 1. Prepare the gelatin-coating solution by dissolving 5 g of gelatin in 1 L of heated, deionized H2O (temperature should not exceed 45 ˚C). 2. After the gelatin has dissolved, add 0.5 g of chromium potassium sulfate. Chromium potassium sulfate will positively charge the slides allowing them to attract negatively charged tissue sections. 3. Filter this solution and store at 2-8 ˚C until use. It is recommended that this solution be filtered again immediately before use (adjust to room temperature before filtration).

COOL & FILTER AT ROOM TEMPERATURE

ADD Gelatin-coating Solution

4. Place the histological slides into metal racks.

DIP RACKS WITH SLIDES 3 to 5 TIMES, 5 SECONDS EACH

Note: The slides should be cleaned by washing them in soapy water and rinsing them thoroughly, first in tap water and finally in deionized water. 5. Dip the racks containing the slides 3 to 5 times (~5 seconds each) into the gelatin-coating solution. 6. Remove the racks containing the slides and let them drain. Blot excess solution from the racks onto filter paper (gently tap the racks against the filter paper for better drainage).

Staining Dish

Histological Slides

7. Place the racks containing the slides on the lab bench and cover them with paper towels to protect them from dust. 8. Dry at room temperature for 48 hours. 9. Dried slides can be put back into the boxes that they arrived in and stored at room temperature until use. Slides intended for cryostat sections can be stored at -20 ˚C.

DRY AT ROOM TEMPERATURE FOR 48 HOURS

STORE AT ROOM TEMPERATURE OR AT -20 ˚C IF INTENDED FOR CRYOSTAT SECTIONS


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IHC/ICC PROTOCOLS C. Chromogenic Staining of Tissue Sections Using HRP-DAB Detection The following protocol has been developed and optimized by R&D Systems IHC/ICC laboratory using the HRP-DAB Cell and Tissue Staining Kits (see page 112). Protocols may need to be modified depending on the type of tissue used. Each investigator should determine the optimal working dilution of an antibody. If using R&D Systems primary antibodies, refer to the product data sheets to obtain approximate working dilutions. For all other reagents follow the manufacturer’s instructions. Caution: DAB is hazardous. Gloves and safety glasses should be worn, and all steps performed inside a fume hood. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS  Aqueous Mounting Medium (Catalog # CTS011, or equivalent)  Primary Antibodies  Cell and Tissue Staining Kit: (See page 112) Anti-goat (Catalog # CTS008), Anti-mouse (Catalog # CTS002), Anti-rabbit (Catalog # CTS005), Anti-rat (Catalog # CTS017) Kit contents: Peroxidase Blocking Reagent, Avidin Blocking Reagent, Biotin Blocking Reagent, Biotinylated Secondary Antibodies, High Sensitivity Streptavidin-HRP Conjugate (HSS-HRP), DAB Chromogen Solution, Serum Blocking Reagents  Deionized H20  Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton™ X-100, and 0.01% sodium azide  PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4  Wash buffer: PBS (1x)  For Paraffin-embedded tissue sections : Xylenes (mixed isomers) 100% denatured alcohol 95% denatured alcohol 70% denatured alcohol 50% denatured alcohol OPTIONAL REAGENTS  Antigen retrieval reagents (see page 17)  DAB Enhancer (Catalog # CTS010)

If necessary, perform antigen retrieval (see page 17 for protocol). If endogenous peroxidase is present in the tissue 3. Incubate the sample with 1-3 drops peroxidase blocking reagent for 5 minutes Note: This procedure may affect the morphology of unfixed frozen tissue sections. Reduction in the incubation time may be required. 4. Rinse the sample, then gently wash in wash buffer for 5 minutes. 5. Surround the tissue with a hydrophobic barrier using a barrier pen. Proceed to the staining protocol on page 15.

CRYOSTAT SECTIONS THAW SLIDES AT ROOM TEMPERATURE 10-20 MINUTES

MATERIALS  Coverslips  Hydrophobic barrier pen  Slides with paraffin-embedded or cryostat tissue sections

PROCEDURE

PARAFFIN-EMBEDDED TISSUE SECTIONS 95% Alcohol

70% Alcohol

50% Alcohol

2 times 2 times 1 time 10 minutes 10 minutes 5 minutes

1 time 5 minutes

1 time 5 minutes

Xylene

100% Alcohol

REHYDRATE THE SLIDES WITH Wash Buffer FOR 5 MINUTES

1. Slide preparation for Cryostat Sections

RINSE WITH Deionized H20

a. When staining cryostat sections stored in a freezer, thaw the slides at room temperature for 10-20 minutes. Slide preparation for Paraffin-embedded Tissue Sections a. Immerse the slides in xylene (mixed isomers) 2 times for 10 minutes each. b. Immerse the slides in 100% alcohol 2 times for 10 minutes each. c. Immerse the slides in 95% alcohol for 5 minutes. d. Immerse the slides in 70% alcohol for 5 minutes. e. Immerse the slides in 50% alcohol for 5 minutes. f. Rinse the slides with deionized H20. 2. Rehydrate the slides with wash buffer for 10 minutes. Drain the excess wash buffer. If proceeding directly to one of the staining protocols on pages 15 or 16, surround the tissue with a hydrophobic barrier using a barrier pen.

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DRAIN EXCESS Wash Buffer AND PERFORM ANTIGEN RETRIEVAL IF NECESSARY

IHC/ICC PROTOCOLS Chromogenic Staining Using HRP-DAB Detection

PROCEDURE 1. Block the slides with 1-3 drops of serum blocking reagent for 15 minutes. Drain the slides and wipe away any excess blocking reagent before proceeding to the next step. Do not rinse.

BLOCKING (FOLLOW STEPS 1-3)

2. Incubate the sample with 1-3 drops of avidin blocking reagent for 15 minutes. Rinse the sample with wash buffer, drain slides, and wipe away any excess wash buffer. 3. Incubate the sample with 1-3 drops of biotin blocking reagent for 15 minutes. Rinse with wash buffer, drain the slides, and wipe away any excess wash buffer. 4. Incubate the sample with primary antibodies in dilution buffer. Follow manufacturer’s recommendations regarding working dilution, incubation time, and temperature. These variables may need to be modified for your system.

RINSE WITH Wash Buffer, DRAIN SLIDES ADD Primary Antibodies INCUBATE THE SAMPLE ACCORDING TO THE MANUFACTURER’S RECOMMENDATIONS

5. Rinse the sample with wash buffer and then wash 3 times in wash buffer for 5 minutes each and drain the slides. 6. Incubate the sample with 1-3 drops of biotinylated secondary antibodies for 30-60 minutes, adjusting the incubation time depending on the thickness of the section (approximately 30 minutes for 5-10 μm thick sections and 60 minutes for 10-20 μm thick sections).

RINSE 3 TIMES FOR 5 MINUTES EACH WITH Wash Buffer, AND DRAIN ADD Biotinylated Secondary Antibodies

INCUBATE FOR 30-60 MINUTES

7. Rinse the sample with wash buffer then wash 3 times in wash buffer for 15 minutes each and drain the slides.

RINSE 3 TIMES FOR 15 MINUTES EACH WITH Wash Buffer, AND DRAIN

8. Incubate the sample with 1-3 drops of HSS-HRP for 30 minutes. 9. Rinse the sample with wash buffer then wash 3 times in wash buffer for 2 minutes each. 10. Calculate the working volume of DAB Chromogen Solution given that 100-200 μL is required to cover the entire tissue section on a slide. Add 1-5 drops of DAB Chromogen Solution to cover the entire tissue section and incubate for 3-20 minutes. Monitor the intensity of the tissue staining under a microscope. Note: DAB Enhancer can be used to intensify the DAB Chromogen solution. DAB is hazardous. Refer to the MSDS for safe deactivation. 11. Rinse the sample with wash buffer 3 times for 10 minutes each.

ADD HSS-HRP

INCUBATE FOR 30 MINUTES

RINSE AND WASH 3 TIMES IN Wash Buffer FOR 2 MINUTES EACH ADD DAB Chromogen Solution

INCUBATE 3-20 MINUTES MONITORING INTENSITY OF TISSUE STAINING UNDER A MICROSCOPE

12. Rinse in deionized H20 and drain the slides. 13. Stained tissue can be mounted either without counterstaining or counterstained with hematoxylin for better visualization of the tissue morphology. 14. Cover stained tissue with a coverslip of an appropriate size. Place slides vertically on a filter paper or towel to drain excess mounting medium and allow them to dry.

RINSE 3 TIMES FOR 10 MINUTES EACH WITH Wash Buffer & 1 TIME WITH Deionized H2O COUNTERSTAIN IF REQUIRED

15. Visualize tissues under a light microscope.


MOUNT WITH Aqueous Mounting Medium

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IHC/ICC PROTOCOLS D. IHC Staining of Tissue Sections for Fluorescence Microscopy The following protocol has been developed and optimized by R&D Systems IHC/ICC laboratory using the NorthernLights fluorescent secondary reagents (see page 110). Protocols may need to be modified depending on the type of tissue used. Each investigator should determine the optimal working dilution of an antibody. If using R&D Systems primary antibodies, refer to the product data sheets to obtain approximate working dilutions. For all other reagents follow the manufacturer’s instructions. Caution: DAB is hazardous. Gloves and safety glasses should be worn, and all steps performed inside a fume hood. Please read the protocol in its entirety before starting.

ADDITIONAL SUPPLIES FOR FLUORESCENT IHC STAINING REAGENTS  Primary Antibodies  Anti-fade Mounting Medium (i-BRITE Plus, Catalog # SF40000, Neuromics, Inc., or equivalent)  Blocking buffer: 10% normal donkey serum, 0.3% Triton X-100  Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton™ X-100, and 0.01% sodium azide  NorthernLights Fluorescent Secondary Antibodies/Streptavidin Conjugates (or equivalent)  PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4  Wash buffer: PBS (1x)

5. Incubate for 1 hour at room temperature with a NorthernLightsconjugated secondary antibody (or equivalent) in dilution buffer designed to recognize the host species of the primary antibody. Alternatively, a NorthernLights-streptavidin conjugate (or equivalent) can be used if the primary antibody is biotinylated.

MATERIALS  Coverslips  Slides with paraffin-embedded or cryostat tissue sections

7. Mount with an anti-fade mounting medium.

Note: Some tissues may bind non-specifically to biotin-avidin labeling components resulting in high background staining. (See page 5) 6. Wash the slides 3 times for 15 minutes each in wash buffer.

BLOCK THE SLIDE WITH Blocking Buffer (STEP 2)

IHC Staining Using Fluorescence Microscopy

PROCEDURE

INCUBATE FOR 15 MINUTES AT ROOM TEMPERATURE

1. Slide preparation (See Step 1 on page 14) 2. Block the slides using blocking buffer for 15 minutes at room temperature and rinse with wash buffer. 3. Make working dilutions of the primary antibodies in dilution buffer according to manufacturer’s instructions. Add small volumes of these dilutions to the slides and incubate at 4 ˚C overnight, or for 30-60 minutes at room temperature.

RINSE WITH Wash Buffer ADD Primary Antibodies INCUBATE SAMPLE AT 4 ˚C OVERNIGHT OR 1 HOUR AT ROOM TEMPERATURE

Note: Multiple primary antibodies to different antigens can be added in step 5 as long as care is taken to avoid the use of secondary reagents with overlapping spectra. If possible, primary antibodies should also be raised in different species. Please see page 8 for more details. Note: A negative control and/or an isotype-matched control should be included to identify non-specific binding from the secondary antibody. Please see page 5 for more details about IHC/ICC controls.

RINSE 3 TIMES IN Wash Buffer FOR 15 MINUTES ADD Secondary Antibodies

4. Wash the slides 3 times for 15 minutes each in wash buffer.

INCUBATE FOR 1 HOUR AT ROOM TEMPERATURE

RINSE 3 TIMES IN Wash Buffer FOR 15 MINUTES MOUNT WITH Anti-fade Medium Triton X-100 is a trademark of Dow Chemical Corporation.

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IHC/ICC PROTOCOLS E. Antigen Retrieval Using Heat-induced Epitope Retrieval (HIER) Antigen retrieval can reveal epitopes masked during the preparation of tissues for staining. The protocol below describes a technique used by R&D Systems and can be used on both cryostat and paraffin-embedded sections. The protocol can be used with R&D Systems Acidic, Basic, or Universal Antigen Retrieval Reagents. Pretreatment with these solutions may induce a dramatic enhancement of immunoreactivity. However, they also have the potential to affect tissue morphology. The optimal method of antigen retrieval must be determined experimentally. For examples of IHC studies using antigen retrieval, see page 10. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS  Antigen Retrieval Solution (10x): Antigen Retrieval Reagent-Basic (Catalog # CTS013) or Antigen Retrieval Reagent-Acidic (Catalog # CTS014) or Antigen Retrieval Reagent-Universal (Catalog # CTS015)  Deionized H2O  PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4 EQUIPMENT  Polypropylene Coplin staining jar (or equivalent)  Water bath at 92-95 ˚C MATERIALS  Slides with paraffin-embedded or cryostat tissue sections

PROCEDURE 1. Make working dilutions by mixing 1 part of 10x Antigen Retrieval concentrate with 9 parts of deionized water.

HEAT SLIDES IN Antigen Retrieval Solution FOR 2-10 MINUTES 92-95 ˚C

Water bath ALLOW TO COOL AT ROOM TEMPERATURE RINSE WITH Deionized H20 AND THEN WITH PBS

2. Preheat retrieval solution to 92-95 ˚C. This may be achieved by placing a polypropylene Coplin staining jar filled with retrieval solution into a water bath.

AVOID VIGOROUS RINSING

Note: Heating may cause cracking of glass staining dishes. 3. Immerse slides into preheated retrieval solution for 2-10 minutes. Note: Since the effect of antigen retrieval reagents depends on their temperature (90-100 ˚C) and incubation time (up to 30 minutes), optimal conditions should be determined by the individual investigator.

Coplin Staining Jar

Note: Cryostat sections are more sensitive to damage by retrieval solution than paraffin-embedded tissues. To avoid tissue damage, it may be necessary to shorten the incubation time to 2-5 minutes. 4. After the incubation is finished, remove the Coplin jar with retrieval solution and slides from the water bath, and let it cool to room temperature.

BEGIN STAINING PROCEDURE

5. Gently rinse the slides with deionized water and then with PBS. Note: Because tissues may be loosened after the retrieval procedure, avoid vigorous rinsing to prevent detachment from the slides. 6. Tissues are now ready for blocking and primary antibody incubation.


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IHC/ICC PROTOCOLS F. Preparation of Coverslips & Cell Fixation for ICC The fundamental principles of IHC experiments using tissue sections also apply to ICC experiments. However, these protocols must be specially adapted for use with cultured cells or cell suspensions. The following protocols describe how to prepare gelatin-coated coverslips and fix cultured cells for ICC experiments. Please read the protocols in its entirety before starting.

Coverslip Preparation Using Gelatin

Preparation & Fixation of Cells on Coverslips

Many cultured cell types do not adhere well to glass coverslips. There are several techniques used to enhance cell adhesion including the use of polyamino acids such as Polyornithine or Poly-L-Lysine, and/or extracellular matrix proteins such as Laminin or Fibronectin. Adding a thin layer of gelatin to a coverslip is also often used to enhance the adhesion of cultured cells to glass.

SUPPLIES REQUIRED REAGENTS  Fixative: 2-4% formaldehyde in PBS  Wash buffer: 0.1% BSA in 1x PBS  Cell culture medium  PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4

SUPPLIES REQUIRED

MATERIALS

REAGENTS  Gelatin-coating solution: 0.1% gelatin in deionized H2O

 Gelatin-coated coverslips in a 24-well plate

PROCEDURE

MATERIALS  Coverslips (Sterilized)  Cell culture plate (6- or 24-well)

1. Culture cells by adding 0.5 mL of culture media containing approximately 5,000 cells to the wells of a cell culture plate containing gelatin-coated coverslips.

PROCEDURE 1. Sterilize coverslips and place them into the wells of a 24-well plate. 2. Add 400 µL of gelatin-coating solution and incubate the coverslips for 10 minutes at room temperature. 3. Remove the gelatin-coating solution and air dry the coverslips for 15 minutes. 4. The dried coverslips can now be stored at room temperature until use.

PLATE CELLS ON GELATIN-COATED COVERSLIPS

CULTURE TO DESIRED DENSITY/AGE

2. When cells have reached the desired density/age, remove the culture media from each well and wash twice with PBS. 3. Add 300-400 µL of 2-4% formaldehyde to each well and incubate for 20 minutes at room temperature. 4. Wash the wells twice with PBS and then cover with 400 µL of wash buffer. The coverslips can be stored at 2-8 ˚C for up to 3 months or they may be stained immediately. Note: Fixation can result in hydrophobic cross-linking of tissue proteins. The time, temperature, pH, and fixative used will determine the degree of cross-linking. Once the fixation protocol has been optimized, the same procedure should be used consistently.

ADD 4% Formaldehyde FOR 20 MINUTES AT ROOM TEMPERATURE

WASH 2 TIMES WITH PBS

18


ADD 400 µL of Wash Buffer

WASH 2 TIMES WITH PBS

STAIN OR STORE AT 2-8 °C FOR UP TO 3 MONTHS

IHC/ICC PROTOCOLS G. Fluorescent ICC Staining of Cultured Cells The following protocol has been developed and optimized using R&D Systems primary antibodies and NorthernLights fluorescent secondary antibodies (see page 8). This protocol may need to be optimized for each specific ICC experiment. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS  Primary Antibodies  Blocking buffer: 10% normal donkey serum, 0.3% Triton X-100  DAPI (4',6-diamidino-2-phenylindole) solution: add 1 μL of 14.3 mM stock for every 5 mL of PBS. Store any unused DAPI at 2-8 ˚C, wrapped in aluminum foil  Deionized H2O  Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton™ X-100, and 0.01% sodium azide  Anti-fade mounting medium  NorthernLights-conjugated secondary reagents (see page 110), or equivalent  PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4  Wash and antibody dilution buffer: 0.1% BSA in PBS (1x)

10. Carefully remove the coverslips from the wells and blot to remove any excess water. Dispense 1 drop of Anti-fade mounting medium onto the microscope slide per coverslip. Mount the coverslip with the cells facing towards the slide. 11. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Slides can also be stored in a slide box at < -20 ˚C for later examination.

BLOCK

MATERIALS  Cell-covered coverslips in a 6- or 24-well plate  Fine tweezers

RINSE FIXED CELLS WITH Wash Buffer

PROCEDURE Note: This protocol is optimized for cells grown on coverslips in a 6- or 24-well plate but can be adapted accordingly.

INCUBATE SAMPLE 45 MINUTES AT ROOM TEMPERATURE ADD Primary Antibodies

1. Wash the coverslips or slides containing the fixed cells two times in 400 µL of wash buffer

INCUBATE OVERNIGHT AT 2-8 ˚C OR FOR 1 HOUR AT ROOM TEMPERATURE

2. Add 400 µL of blocking buffer and block for 45 minutes at room temperature. 3. Remove blocking buffer. No rinsing is necessary.

ADD Secondary Antibodies

4. Dilute the unconjugated primary antibody (or fluorescenceconjugated primary) in dilution buffer according to the manufacturer’s instructions. Incubate overnight at 2-8 ˚C, or alternatively, incubate at room temperature for 1 hour. Note: Experimentation with both antibody concentration and incubation time may be required to achieve optimal labeling.

NO RINSING IS NECESSARY

RINSE 2 TIMES WITH Wash Buffer

INCUBATE FOR 1 HOUR ADD DAPI Counterstain

5. Wash two times in 400 µL of wash buffer. If using a primary antibody with a direct fluorescent conjugate, go to step 8. 6. Dilute the secondary antibody in dilution buffer according to the manufacturer’s instructions. Add 400 µL to the wells and incubate at room temperature for 1 hour in the dark. From this step forward samples should be protected from light. 7. Wash two times in 400 µL of wash buffer. 8. Add 300 µl of the diluted DAPI solution to each well and incubate 2-5 minutes at room temperature. DAPI binds to DNA and is a convenient nuclear counterstain. It has an absorption maximum at 358 nm and fluoresces blue at an emission maximum of 461 nm.

RINSE 2 TIMES WITH Wash Buffer

INCUBATE 2-5 MINUTES AT ROOM TEMPERATURE RINSE 1 TIME WITH PBS RINSE 1 TIME WITH Deionized H2O REMOVE THE COVERSLIP WITH A FINE TWEEZERS MOUNT WITH THE CELLS FACING THE SLIDE

9. Wash once with PBS and once with water.


19

IHC/ICC PROTOCOLS H. Preparation of a Cell Smear for Non-adherent Cell ICC One method of preparing non-adherent cells for ICC involves smearing the sample across a gelatincoated slide. This technique forms a monolayer of cells that can be easily visualized. Please read the protocol in its entirety before starting.

SUPPLIES REQUIRED REAGENTS  Deionized H2O  Fixative: 4% formaldehyde in PBS (1x)  PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4

FIX CELLS FOR 20 MINUTES AT ROOM TEMPERATURE

SPIN DOWN CELLS FOR 30 SECONDS

RESUSPEND IN 1 mL Deionized H2O

MATERIALS  Microfuge tube (1.5 mL)  Gelatin-coated microscope slides  Hot plate  Hydrophobic barrier pen  Microfuge

PROCEDURE

SPIN DOWN CELLS FOR 30 SECONDS

1. Fix the cells with 4% formaldehyde for 20 minutes at room temperature by adding formaldehyde directly to the culture media (see page 4) and adjust to approximately 1 x 10 6 cells/mL. 2. Add 1 mL of the cell solution to a 1.5 mL microfuge tube. 3. Spin down the cells for 30 seconds in a microfuge. 4. Pour off the supernatant and resuspend the cell pellet in 1 mL of deionized H2O. 5. Spin down the cells for 30 seconds in the microfuge.

ADD 5 µL Cell Suspension TO GELATIN-COATED SLIDE

6. Pour off the supernatant and resuspend the cell pellet in 200 µL of deionized H2O. 7. Add 5 µL of the cell suspension to a gelatin-coated slide (3 spots per slide) and smear with the side of a pipette tip. 8. Place the slide on a hot plate (low heat setting) and allow the liquid to evaporate. 9. Check the slide under a microscope and make sure that there are no salt crystals. If salt crystals are observed, wash the slide with water.

SMEAR WITH A PIPETTE TIP

10. Surround the cell spot with a hydrophobic barrier using a barrier pen and air dry. 11. The slides can be stored at 2-8 ˚C for up to 3 months or they may be stained immediately.

HEAT TO EVAPORATE THE LIQUID

SURROUND CELLS WITH A Hydrophobic Barrier

20


RESUSPEND IN 200 µL Deionized H2O

TROUBLESHOOTING GUIDE Problem: Lack of Staining Possible Source

Test or Action

Lack of antigen.

Check protein expression by in situ hybridization (in some rare cases translation may be blocked even though mRNA is detected).

Antibodies do not work due to improper storage.

Follow storage instructions on the datasheet. In general, aliquot antibodies into smaller volumes sufficient to make a working solution for a single experiment. Store aliquots in a manual defrost freezer (-20 to -70 ˚C) and avoid repeated freeze-thaw cycles.

Inactive primary or secondary antibodies.

Test reporter system independently to assess reagent viability.

Inadequate tissue fixation.

Try increasing the fixation time or try a different fixative.

Tissue overfixation.

Reduce the duration of the immersion or post-fixation steps. If immersion fixation cannot be avoided (for example, collection of postmortem tissues or biopsies in pathology lab), antigens may be unmasked by treatment with antigen retrieval reagents.

Incompatible secondary and primary antibodies.

Use a secondary antibody that will interact with the primary antibody. For example, if the primary antibody was raised in rabbits, use an anti-rabbit secondary antibody.

Antigen was destroyed before incubation with the primary antibody.

If quenching of endogenous peroxidase was done prior to the addition of primary antibodies, block peroxidase after incubation with the primary antibody.

Epitope altered during fixation or embedding procedure.

Try restoring immunoreactivity through various antigen retrieval techniques. Embed tissue at 58 ˚C or below.

Antigen retrieval was ineffective.

Increase the time of treatment or change the treatment solution.

Reagents omitted or used in wrong order.

Repeat staining and confirm that correct reagents are used and are added in the correct order.

Problem: High Background Possible Source

Test or Action

High concentration of primary and or secondary antibodies.

Titer antibody to determine optimal concentration needed to promote a specific reaction of the primary and the secondary antibodies.

Hydrophobic interactions of the antibody and proteins in the tissue.

Lower the ionic strength of the antibody diluent (particularly monoclonal antibodies respond well to reducing the salt concentration).

Non-specific binding of primary and/or secondary reagents to tissues.

Use blocking step just prior to primary antibody incubation (we use 1% bovine serum albumin with 10% normal donkey serum, or non-fat dry milk is another option).

Non-specific binding of secondary antibody.

Use an antibody that has cross-reactive IgG species removed (absorbed against sample species).

Tissue dried out.

Avoid letting the tissue dry during the staining procedure.

Reagents sticking to old or poorly prepared slides.

Start over with freshly prepared or purchased slides.

Background due to ionic interactions.

Increase the ionic strength of the diluent buffer.

Problem: Cell/Tissue Morphology Destroyed Possible Source

Test or Action

Antigen retrieval methods are too harsh.

Empirically determine the conditions that preserve tissue morphology while restoring the immunoreactivity of the antigen.

Tissue sections falling off slide. (more common with frozen sections)

Increase fixation time. Empirically determine an additional or alternative fixative. Use freshly prepared, adequately charged slides.

Tissue section appears torn or folded. Air bubbles under section.

Re-cut sections using a sharp blade, or ignore damaged areas when analyzing the results.

Poor resolution of tissue morphology

Cut thinner tissue sections. Ice crystals may have destroyed morphology of frozen sections.

Underfixation has physically damaged the tissue or cells during the staining process

Increase fixation time and/or add a post-fixation step. Increase the fixative/tissue ratio. Cut smaller pieces of tissue for more thorough immersion fixation.

Autolysis of tissue leading to staining of necrotic debris.

Increase the fixation time, ratio. Consider using cross-linking fixative.

Problem: Staining is Inappropriate

Possible Source

Test or Action

Fixation method is inappropriate for the antigen.

Try a different fixative or increase the fixation time.

Antigen retrieval may be inappropriate for this antigen or tissue.

Try different antigen retrieval conditions.

Electrostatic charge of the antigen has been altered.

Try adjusting the pH or cation concentration of the antibody diluent.

Delay in fixation caused diffusion of the antigen.

Fix tissue promptly. Try a cross-linking fixative rather than organic (alchohol) fixative.


21

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE

2B4/CD244/SLAMF4

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF1039

100 µg

$355

None

Polyclonal

Goat IgG

AF1050

100 µg

$355

Mouse

SIZE

PRICE

Biotin

Polyclonal

Goat IgG

BAF1050

50 µg

$380

Human

None

Polyclonal

Goat IgG

AF838

100 µg

$355

Human

Biotin

Polyclonal

Goat IgG

BAF838

50 µg

$380

4-Hydroxynonenal

Multi-species

None

198960

Mouse IgG2B

MAB3249

100 µg

$240

5HT1B

Human

None

499325

Mouse IgG1

MAB5858

100 µg

$240

None

Polyclonal

Sheep IgG

AF4975

100 µg

$355

None

524731

Mouse IgG1

MAB4975

100 µg

$240

None

Polyclonal

Sheep IgG

AF5049

100 µg

$355

None

2E2

Mouse IgG2B

4354-MC-050

50 µL

$415

None

105

Mouse IgM

MAB1416

100 µg

$295

4-1BB/TNFRSF9/CD137

5T4

Human Mouse

5T4 in Human Placenta. 5T4 was detected in paraffin-embedded human placenta sections using antihuman 5T4 affinity-purified polyclonal antibody (Catalog # AF4975). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

8-oxo-dG A2B5

Human/Mouse/Rat/Chicken

A2B5 in Human differentiating NTera2 Cells. NTera2 neuronal carcinoma cells were differentiated with retinoic acid for 10 days. A2B5 was detected using anti-human/mouse/rat/chicken A2B5 monoclonal antibody (Catalog # MAB1416). The cells were stained using NorthernLights-557 anti-mouse IgG secondary antibody (Catalog # NL007; red) and the nuclei counterstained with DAPI (blue).

a1-Acid Glycoprotein a-2-Macroglobulin

a2u-Globulin

A33

Human Human

Rat

Human Mouse

ABCG2

ACE/CD143

Human

Human

Mouse

None

386131

Mouse IgG2A

MAB3694

100 µg

$240

None

Polyclonal

Goat IgG

AF1938

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1938

50 µg

$380

None

Polyclonal

Goat IgG

AF586

100 µg

$355

Biotin

129736

Mouse IgG1

BAM586

250 µg

$380

None

129736

Mouse IgG1

MAB586

500 µg

$325

None

Polyclonal

Goat IgG

AF3080

100 µg

$355

None

402104

Rat IgG2A

MAB3080

100 µg

$240

None

Polyclonal

Goat IgG

AF2756

100 µg

$355

Biotin

5D3

Mouse IgG2B

BAM995

250 µg

$380

None

5D3

Mouse IgG2B

MAB995

500 µg

$325

None

Polyclonal

Goat IgG

AF929

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF929

50 µg

$380

None

Polyclonal

Goat IgG

AF1513

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1513

50 µg

$380

ACE in Human Kidney. Angiotensin Converting Enzyme (ACE) was detected in paraffin-embedded human kidney tissue sections using anti-human ACE polyclonal antibody (Catalog # AF929). Tissues were stained using the Goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

22



ACE-2

SPECIES

Human

Mouse

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF933

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF933

50 µg

$380

None

171606

Mouse IgG2A

MAB933

500 µg

$325

None

Polyclonal

Goat IgG

AF3437

100 µg

$355

Polyclonal

Sheep IgG

AF4000

100 µg

$355

ACE-2 in Mouse Kidney. Angiotensin Converting Enzyme-2 (ACE-2) was detected in a cryostat section of mouse kidney using anti-mouse affinity-purified ACE-2 polyclonal antibody (Catalog # AF3437; red). The tissue was counterstained green.

Actin

Human/Mouse/Rat

None

Activin A bA subunit

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF338

100 µg

$355

Activin C bC subunit

Human

None

Polyclonal

Goat IgG

AF1629

100 µg

$355

Activin RIA/ALK-2

Human

None

Polyclonal

Goat IgG

AF637

100 µg

$355

None

71412

Mouse IgG1

MAB637

500 µg

$325

Activin RIB/ALK-4

Mouse

None

Polyclonal

Goat IgG

AF1477

100 µg

$355

Activin RIIA

Human

None

Polyclonal

Goat IgG

AF340

100 µg

$355

Activin RIIB

Human

None

Polyclonal

Goat IgG

AF339

100 µg

$355

ADAM8 Ectodomain

Human

None

Polyclonal

Goat IgG

AF1031

100 µg

$355

ADAM9 Ectodomain ADAM10 Ectodomain

ADAM15 Ectodomain

Mouse

None

Polyclonal

Goat IgG

AF949

100 µg

$355

Human

None

Polyclonal

Total Goat IgG

AB936

1 mg

$355

Mouse

None

Polyclonal

Total Goat IgG

AB946

1 mg

$355

None

Polyclonal

Goat IgG

AF935

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF935

50 µg

$380

None

23G9

Mouse IgG1

MAB935

500 µg

$325

None

Polyclonal

Goat IgG

AF945

100 µg

$355

504425

Mouse IgG2A

MAB4974

100 µg

$240

539220

Mouse IgG1

MAB5380

100 µg

$240

Human

Mouse

ADAM15 Expression in Mouse Cerebellum. A dis integin and mettalloprotease domain 15 (ADAM15) was detected in cryostat sections of mouse cerebellar Purkinje cells using anti-mouse ADAM15 polyclonal antibody (Catalog # AF945; red). The tissue was counterstained green.

ADAM23 aa 60-585

Human

None

ADAM23 in Human Spleen. A disintegrin and metalloprotease domain 23 (ADAM23) was detected in paraffin-embedded human spleen sections using anti-human ADAM23 monoclonal antibody (Catalog # MAB4974). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

ADAM32 aa 17-476

Human

None


23

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE ADAMTS1 Propeptide

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Goat IgG

AF3079

100 µg

$355

Polyclonal

Goat IgG

AF1119

100 µg

$355

ADAMTS1 in Human Placenta. A disintegrin-like and mettaloprotease with thrombospondin motifs (ADAMTS1) was detected in a paraffin-embedded human placenta section using anti-human ADAMTS1 affinity-purified polyclonal antibody (Catalog # AF3079). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Adiponectin/Acrp30 Adiponutrin/ADPN

Mouse

None

Rat

None

Polyclonal

Goat IgG

AF3100

100 µg

$355

Human

None

Polyclonal

Sheep IgG

AF5208

100 µg

$355

Polyclonal

Chicken IgY

AF1369

100 µg

$355 $240

ADPN in Human Adipocytes. Adiponutrin (ADPN) was detected in human adipocytes using anti-human ADPN affinity-purified polyclonal antibody (Catalog # AF5208). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and the nuclei counterstained with DAPI (blue).

None

Human a-Fetoprotein/AFP

Aggrecan G1-IGD-G2 Domains

AgRP/ART

None

189506

Mouse IgG1

MAB1369

100 µg

Human/Mouse

None

189502

Mouse IgG1

MAB1368

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF5369

100 µg

$355

None

Polyclonal

Goat IgG

AF1220

100 µg

$355

None

179509

Mouse IgG2B

MAB1220

500 µg

$325

Human

None

Polyclonal

Goat IgG

AF704

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF634

100 µg

$355

None

Polyclonal

Rabbit IgG

AF1457

100 µg

$285

None

Polyclonal

Sheep IgG

AF5824

100 µg

$355 $240

Human

AgRP in Mouse Thalamus. Agouti-Related Protein (AgRP) was detected in a cryostat section of mouse thalamus using anti-mouse AgRP monoclonal antibody (MAB634; red). The tissue was counterstained green.

AIF

Human/Mouse/Rat

a-Internexin

Human

None

529724

Mouse IgG1

MAB4844

100 µg

Akt Pan Specific

Human/Mouse/Rat

None

281046

Mouse IgG2B

MAB2055

100 µg

$240

Phospho-Akt (S473) Pan Specific

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF887

100 µg

$285

Akt1

Human

None

302407

Mouse IgG1

MAB17751

100 µg

$240

Human

None

302501

Mouse IgG1

MAB23152

100 µg

$240

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF23151

100 µg

$355

Human/Mouse

None

188835

Mouse IgG2A

MAB1455

100 µg

$240

None

Polyclonal

Goat IgG

AF1172

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1172

50 µg

$380

None

Polyclonal

Goat IgG

AF577

100 µg

$355

Biotin

118311

Mouse IgG2A

BAM5771

100 µg

$240

Akt2 Serum Albumin ALCAM/CD166

Mouse

ALK-7

Rat

24


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Alkaline Phosphatase/ALPL

SPECIES Human/Mouse/Rat

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Biotin

B4-78

Mouse IgG1

BAM1448

100 µg

$240

None

B4-78

Mouse IgG1

MAB1448

100 µg

$240

Alkaline Phosphatase and Oct-3/4 in Human BG01V Embryonic Stem Cells. Alkaline Phosphatase was detected in the human BG01V embryonic stem cell line using anti-human/mouse/rat Alkaline Phosphatase monoclonal antibody (Catalog # MAB1448). Oct-3/4 was detected using anti-human Oct-3/4 polyclonal antibody (Catalog # AF1759). The cells were stained for Alkaline Phosphatase using NorthernLights-557 anti-mouse secondary antibody (Catalog # NL007; pseudocolored green) and stained for Oct-3/4 using NorthernLights-637 anti-goat secondary antibody (Catalog # NL002; red).

AMIGO2

Human

None

273610

Mouse IgG3

MAB20801

100 µg

$240

AMIGO3

Mouse

None

371301

Rat IgG2A

MAB2375

100 µg

$240

AMIGO3 in Mouse Cerebellum. Amphoterin-induced Gene and ORF 3 (AMIG03) was detected in Purkinje neuron cell bodies in a cryostat section of mouse cerebellum using anti-mouse AMIG03 monoclonal antibody (Catalog # MAB2375).

Aminopeptidase N/ANPEP

Human

None

498001

Mouse IgG2A

MAB3815

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF2335

100 µg

$355

AF4647

100 µg

$355

Aminopeptidase N/CD13 in Human Liver Cancer. Aminopeptidase N (APN) was detected in paraffinembedded human liver cancer sections using antihuman APN monoclonal antibody (Catalog # MAB3815). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Cata log # CTS002; brown) and counterstained with hematoxylin (blue).

Aminopeptidase O/ONPEP

Human

None

Polyclonal

Goat IgG

Aminopeptidase P1/XPNPEP1

Human

None

409124

Mouse IgG1

MAB29701

100 µg

$240

None

Polyclonal

Total Goat IgG

AB-262-NA

1 mg

$355

None

Polyclonal

Goat IgG

AF262

100 µg

$355

Amphiregulin

AMPKa1 aa 340-550

Human Mouse

None

Polyclonal

Goat IgG

AF989

100 µg

$355

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF3197

100 µg

$355

AMPKa2 aa 340-552

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF2850

100 µg

$355

AMPKb1

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF2854

100 µg

$355

Androgen R/NR3C4

Human

None

H7507

Mouse IgG2A

PP-H7507-00

100 µg

$380

Angiogenin

Human

None

Polyclonal

Goat IgG

AF265

100 µg

$355

Angiopoietin-1

Human

None

Polyclonal

Goat IgG

AF923

100 µg

$355

None

171718

Mouse IgG2B

MAB923

500 µg

$325


25

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Angiopoietin-2

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Goat IgG

AF623

100 µg

$355

Polyclonal

Goat IgG

AF738

100 µg

$355

Angiopoietin-2 in Human Gastrointestinal Cancer. Angiopoietin-2 was detected in paraffin-embedded human gastrointestinal cancer sections using anti-human Angiopoietin-2 affinity-purified polyclonal antibody (Catalog # AF623). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Angiopoietin-3

Mouse

None

Angiopoietin-4

Human

None

Polyclonal

Goat IgG

AF964

100 µg

$355

Angiopoietin-like 1

Human

None

Polyclonal

Goat IgG

AF2204

100 µg

$355

Angiopoietin-like 2

Mouse

None

Polyclonal

Goat IgG

AF1444

100 µg

$355

Angiopoietin-like 3

Mouse

None

Polyclonal

Goat IgG

AF136

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF136

50 µg

$380

ANP

Human

None

361101

Rat IgG2B

MAB3366

100 µg

$240

AP-2g

Human

None

Polyclonal

Goat IgG

AF5059

100 µg

$355

APC C-Terminus

Human

None

Polyclonal

Goat IgG

AF3695

100 µg

$355

APJ

Human

None

72133

Mouse IgG3

MAB856

500 µg

$325

APLP-1

Human

None

Polyclonal

Goat IgG

AF3129

100 µg

$355

None

354008

Mouse IgG2B

MAB3129

100 µg

$240

None

Polyclonal

Goat IgG

AF3556

100 µg

$355

None

369717

Mouse IgG2B

MAB4124

100 µg

$240

Apolipoprotein B/ApoB

Human

APP Pan Specific

Human

None

Polyclonal

Goat IgG

AF1168

100 µg

$355

Phospho-APP (T668)

Human/Mouse

None

304904

Mouse IgG1

MAB2508

100 µg

$240

Phospho-Amyloid Precursor Protein (668T) in Human Alzheimer’s Disease Brain. Amyloid precursor protein (APP) phosphorylated at T668 was detected in paraffin-embedded human Alzheimer’s disease brain sections using anti-human phospho-APP monoclonal antibody (Catalog # MAB2508). Tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

ARC aa 176-195

Human/Mouse/Rat

b-Arrestin 1

Human

b-Arrestin 2

Human Human

Artemin

Mouse

None

Polyclonal

Rabbit IgG

AF636

100 µg

$285

None

Polyclonal

Goat IgG

AF4560

100 µg

$355

None

425817

Rat IgG2A

MAB45601

100 µg

$240

None

Polyclonal

Goat IgG

AF4564

100 µg

$355

None

Polyclonal

Goat IgG

AF2589

100 µg

$355

None

Polyclonal

Goat IgG

AF1085

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1085

50 µg

$380

None

185234

Rat IgG2A

MAB10851

500 µg

$325

Arylsulfatase A/ARSA

Human

None

Polyclonal

Goat IgG

AF2485

100 µg

$355

ASK1 aa 1011-1196

Human

None

Polyclonal

Sheep IgG

AF3575

100 µg

$355

Phospho-ATF2 (S490/S498)

Human/Rat

None

Polyclonal

Rabbit IgG

PPS064

100 µL

$355

Axl

Human

None

Polyclonal

Goat IgG

AF154

100 µg

$355

26


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Phospho-Axl (Y779)

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Rabbit IgG

AF2228

100 µg

$285

RA3-6B2

Rat IgG2A

BAM1217

250 µg

$380

Phospho-Axl (Y779) in Human Stomach. PhosphoAxl was detected in paraffin-embedded human stomach sections using anti-human/mouse phospho-Axl affinity-purified polyclonal antibody (Catalog # AF2228). Tissue was stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).

B220/CD45R

Mouse

Human B7-1/CD80

Mouse Rat Human

B7-2/CD86

Rat

Biotin None

RA3-6B2

Rat IgG2A

MAB1217

500 µg

$325

None

Polyclonal

Goat IgG

AF140

100 µg

$355

None

37711

Mouse IgG1

MAB140

500 µg

$325

None

Polyclonal

Goat IgG

AF740

100 µg

$355

None

Polyclonal

Goat IgG

AF1214

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1214

50 µg

$380

None

Polyclonal

Goat IgG

AF-141-NA

200 µg

$230

None

Polyclonal

Goat IgG

AF1340

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1340

50 µg

$380

B7-H1/PD-L1

Mouse

None

Polyclonal

Goat IgG

AF1019

100 µg

$355

B7-H2

Mouse

None

Polyclonal

Goat IgG

AF158

100 µg

$355

B7-H3

Human

None

Polyclonal

Goat IgG

AF1027

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1027

50 µg

$380

BACE-1 Ectodomain

Human/Mouse

None

137612

Mouse IgG1

MAB931

500 µg

$325

BACE-2 Ectodomain

Human

None

391017

Rat IgG2A

MAB4097

100 µg

$240

Human

None

Polyclonal

Goat IgG

AF124

100 µg

$355

BAFF/BLyS/TNFSF13B

Mouse

Biotin

Polyclonal

Goat IgG

BAF2106

50 µg

$380

BAI1

Human

None

542515

Mouse IgG1

MAB4969

100 µg

$240

BAI3

Human

None

409633

Mouse IgG1

MAB39651

100 µg

$240

BAMBI/NMA

Human

None

Polyclonal

Goat IgG

AF921

100 µg

$355

Bax aa 12-33

Human/Mouse

None

Polyclonal

Rabbit IgG

AF820

30 µg

$285

Bax Minus C-Terminus

Human

None

Polyclonal

Total Goat IgG

AB820

400 µg

$395

Bcl-2 aa 1-208

Human/Mouse

None

Polyclonal

Goat IgG

AF810

100 µg

$355

Bcl-w aa 1-172

Human/Mouse

None

Polyclonal

Goat IgG

AF8241

100 µg

$355

Bcl-xL aa 3-14

Human/Mouse/Rat

None

YTH-2H12

Mouse IgG2A

2300-MC-100

100 µg

$285

None

Polyclonal

Chicken IgY

AF248

100 µg

$355

BDNF

Human

Biotin

Polyclonal

Chicken IgY

BAF248

50 µg

$380

None

35928

Mouse IgG1

MAB248

500 µg

$325

BDNF in Rat Spinal Cord and Dorsal Root Ganglia. Brain-derived neurotrophic factor (BDNF) was detected in rat dorsal root ganglion using anti-human BDNF affinity-purified polyclonal antibody (Catalog # AF248; red) and counterstained green.

Human Betacellulin/BTC

bIG-H3

Mouse Mouse

None

Polyclonal

Goat IgG

AF-261-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF1025

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1025

50 µg

$380

None

Polyclonal

Sheep IgG

AF2559

100 µg

$355


27

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Biglycan

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Goat IgG

AF2667

100 µg

$355

Polyclonal

Sheep IgG

AF5474

100 µg

$355

Biglycan in Human Skin. Biglycan was detected in paraffin-embedded human skin sections using antihuman Biglycan affinity-purified polyclonal antibody (Catalog # AF2667). Tissue was stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

BIK

Human

None

BMI-1

Human

None

Polyclonal

Goat IgG

AF3334

100 µg

$355

BMP-1/PCP

Human

None

Polyclonal

Goat IgG

AF1927

100 µg

$355

Pro BMP-2

Human

None

253717

Mouse IgG2A

MAB2260

500 µg

$325

BMP-2/BMP-4

Human

None

Polyclonal

Goat IgG

AF355

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF355

50 µg

$380

BMP-3

Human

None

Polyclonal

Goat IgG

AF113

100 µg

$355

BMP-3b/GDF-10

Human

BMP-5

Human

BMP-6

Human

None

Polyclonal

Goat IgG

AF1543

100 µg

$355

None

Polyclonal

Goat IgG

AF615

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF615

50 µg

$380

None

Polyclonal

Goat IgG

AF507

100 µg

$355

None

164311

Mouse IgG2B

MAB3541

500 µg

$325

None

164313

Mouse IgG1

MAB3542

500 µg

$325

BMP-7

Human

BMP-8

Human

None

Polyclonal

Goat IgG

AF1073

100 µg

$355

Human

None

317809

Rat IgG2A

MAB2925

100 µg

$240

MAB24061

100 µg

$240

BMP-15/GDF-9B aa 268-392

BMP-15/GDF-9b in Mouse Ovary. Bone Morphogenetic Protein-15 (BMP-15) was detected in a frozen section of adult mouse ovary using anti-human BMP15 monoclonal antibody (Catalog # MAB2925). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (red; Catalog # NL013) and counterstained with DAPI (blue).

BMPR-IA/ALK-3

Human

None

550607

Mouse IgG2B

BMPR-IB/ALK-6

Human

None

477914

Mouse IgG2B

MAB5051

100 µg

$240

None

Polyclonal

Goat IgG

AF811

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF811

50 µg

$380

BMPR-II

Human

BMPR-II in Mouse Embryo. BMPR-II was detected in a frozen section of E15 mouse ribs using anti-human BMPR-II affinity-purified polyclonal antibody (Catalog # AF811; green).

BNP Precursor

Human

None

370124

Rat IgG2B

MAB36041

100 µg

$240

BOC

Mouse

None

Polyclonal

Goat IgG

AF2385

100 µg

$355

28


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Brachyury

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF2085

Brachyury in Differentiated Human Embryonic Stem cells. Brachyury was detected in differentiated human Embryonic Stem cells using anti-human Brachyury affinity-purified polyclonal antibody (Catalog # AF2085). Cells were stained using an anti-goat secondary antibody (green) and counterstained with DAPI (blue). Courtesy of Dr. Frank Solder from the National Institute of Neurological Disorders and Stroke & Stem Cell Unit at NIH.

Brachyury NorthernLights-conjugated

Human

NL557

SIZE

PRICE

100 µg

$355

Brachyury in Embryonic Mouse Notochord. Brachyury was detected in E9.5 mouse notochord using anti-human Brachyury affinity-purified polyclonal antibody (Catalog # AF2085). The cells were stained using NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Polyclonal

Goat IgG

NL2085R

500 µL

$350 $355

Human

None

Polyclonal

Goat IgG

AF2210

100 µg

Human/Mouse/Rat

None

265402

Mouse IgG2A

MAB2210

100 µg

$240

Phospho-BRCA1 (S1423)

Human

None

Polyclonal

Rabbit IgG

AF1386

100 µg

$285

BRCA2

Human

None

234403

Mouse IgG1

MAB2476

100 µg

$240

None

Polyclonal

Sheep IgG

AF4009

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF4009

50 µg

$380

BRCA1

Brevican

Human

Brk aa 1-70

Human

None

Polyclonal

Goat IgG

AF3966

100 µg

$355

BSRP-A

Mouse

None

529805

Rat IgG2A

MAB4916

100 µg

$240

AF2379

100 µg

$355

BSRP-A in Mouse Hippocampus. Brain-specific receptor-like protein A (BSRP-A) was detected in a cryostat section of mouse hippocampus using anti mouse BSRP-A monoclonal antibody (Catalog # MAB4916). The tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

C1q R1/CD93 C4.4A/LYPD3

Cadherin-6/KCAD

Human

None

Polyclonal

Goat IgG

Mouse

None

223437

Rat IgG2B

MAB1696

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF5567

100 µg

$355

None

Polyclonal

Sheep IgG

AF2715

100 µg

$355

None

427909

Mouse IgG1

MAB2715

100 µg

$240

None

427903

Mouse IgG3

MAB27151

100 µg

$240

Human

K-Cadherin in Human Liver Cancer. K-Cadherin was detected in paraffin-embedded human liver cancer sections using anti-human K-cadherin monoclonal antibody (Catalog # MAB27151). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Cadherin-11

Human

None

Polyclonal

Goat IgG

AF1790

100 µg

$355

Cadherin-12

Human

None

343621

Rat IgG1

MAB2240

100 µg

$240


29

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Cadherin-13

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Goat IgG

AF3264

100 µg

$355

None

Polyclonal

Goat IgG

AF1032

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1032

50 µg

$380

None

141713

Mouse IgG1

MAB1032

500 µg

$325

None

Polyclonal

Goat IgG

AF648

100 µg

$355

None

180215

Mouse IgG2B

MAB1838

500 µg

$325

None

180224

Mouse IgG2B

MAB18381

100 µg

$240

None

Polyclonal

Goat IgG

AF748

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF748

50 µg

$380

None

114420

Rat IgG2A

MAB7481

100 µg

$240

Polyclonal

Sheep IgG

AF1388

100 µg

$355

Cadherin-13 in Human Lung Cancer Cells. Cadherin-13 was detected in NCI-H460 human lung cancer cell line using anti-human Cadherin-13 affinitypurified polyclonal antibody (Catalog # AF3264). Cells were stained using NorthernLights-557 antigoat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Cadherin-17

Human

Human E-Cadherin Mouse

E-Cadherin and SOX2 in the BG01V Human Embryonic Stem Cell Line. E-Cadherin and SOX2 were detected in human BG01V cells using antihuman E-Cadherin affinity-purified polyclonal antibody (Catalog # AF648; red) and anti-human/ mouse SOX2 monoclonal antibody (Catalog # MAB2018; green). Cells were stained using anti-goat NorthernLights-557 (Catalog # NL001) and antimouse NorthernLights-493 (Catalog # NL009). Cells were counterstained with DAPI (blue).

N-Cadherin

Human

None

N-Cadherin in Human Hippocampus. N-Cadherin was detected in a paraffin-embedded section of human hippocampus using anti-human N-Cadherin affinity-purified polyclonal antibody (Catalog # AF1388). The tissue was stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).

Human P-Cadherin

VE-Cadherin

Calbindin D

Mouse

Human

Human

None

104805

Mouse IgG1

MAB861

500 µg

$325

None

Polyclonal

Goat IgG

AF761

100 µg

$355

None

106020

Rat IgG2A

MAB761

500 µg

$325

None

Polyclonal

Goat IgG

AF938

100 µg

$355

None

123413

Mouse IgG2B

MAB9381

100 µg

$240

None

Polyclonal

Goat IgG

AF3320

100 µg

$355

None

401025

Mouse IgG2B

MAB3320

100 µg

$240

Calbindin D in Rat Hypothalamus. Calbindin D was detected in a frozen rat hypothalamus section using anti-human calbindin D monoclonal antibody (Catalog # MAB3320). Tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

30


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Calretinin

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF5065

100 µg

$355

AF5244

100 µg

$355

Calretinin/Calbindin-2 in Human Brain Cortex. Calretinin was detected in paraffin-embedded human brain cortex sections using anti-human/mouse/ rat Calretinin affinity-purified polyclonal antibody (Catalog # AF5065). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Calsyntenin-3

Human

None

Polyclonal

Sheep IgG

CaM Kinase IIa

Human

None

525501

Mouse IgG2B

MAB5584

100 µg

$240

CaM Kinase IId aa 402-478

Human

None

Polyclonal

Sheep IgG

AF4176

100 µg

$355

CaMKIIδ in Human Cerebral Cortex. Calcium/ Calmodulin-dependent protein kinase type II delta (CaMKIId) was detected in paraffin-embedded hum a n cerebral cortex tissue sections using anti-human CaMKIId affinity-purified polyclonal antibody (Catalog # AF4176). Tissues were stained using antisheep HRP-DAB detection (brown) and counterstained with hematoxylin (blue).

cAMP

Multi-species

None

250532

Mouse IgG1

MAB2146

500 µg

$325

Cannabinoid R2/CB2/CNR2

Human

None

352114

Mouse IgG2A

MAB3655

100 µg

$240

CAR/NR1I3

Human

None

N4111

Mouse IgG1

PP-N4111-00

100 µg

$380

Carbonic Anhydrase II

Human

None

Polyclonal

Sheep IgG

AF2184

100 µg

$355

None

Polyclonal

Goat IgG

AF2186

100 µg

$355

None

310413

Mouse IgG2B

MAB2186

100 µg

$240

None

Polyclonal

Goat IgG

AF2414

100 µg

$355

Carbonic Anhydrase IV

Human Mouse

Carbonic Anhydrase IX

Carnosine Dipeptidase 1/CNDP1

Human

None

Polyclonal

Goat IgG

AF2188

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF2344

100 µg

$355

None

Polyclonal

Goat IgG

AF2489

100 µg

$355

None

352216

Mouse IgG1

MAB2489

100 µg

$240

None

Polyclonal

Goat IgG

AF163

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF163

50 µg

$380

Human

CART

Human

CART aa 28-116

Human

None

113612

Mouse IgG2B

MAB163

500 µg

$325

Active Caspase-3 aa 163-175

Human/Mouse

None

Polyclonal

Rabbit IgG

AF835

50 µg

$285

Cleaved Caspase-3 (Asp175)

Human/Mouse

None

269518

Rabbit IgG

MAB835

50 µg

$270

Caspase-9 aa 1-134

Human

None

LAP6

Mouse IgG1

MAB8301

100 µg

$240

Caspase-9 aa 139-330

Human

None

Polyclonal

Goat IgG

AF8301

100 µg

$355

Human

None

Polyclonal

Sheep IgG

AF5145

100 µg

$355

Caspr2

Caspr2 in Mouse Neurons. Caspr2 was detected in a frozen section of adult mouse brain using anti-human Caspr2 affinity-purified polyclonal antibody (Catalog # AF5145). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).


31


SPECIES Human

b-Catenin

Human/Mouse/Rat

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

196618

Mouse IgG3

MAB13291-050

50 µg

$150

None

196618

Mouse IgG3

MAB13291-100

100 µg

$240

None

Polyclonal

Goat IgG

AF1329

100 µg

$355

None

Polyclonal

Goat IgG

AF1049

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1049

50 µg

$380

None

191424

Rat IgG2B

MAB11231

500 µg

$325

None

Polyclonal

Goat IgG

AF953

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF953

50 µg

$380

None

Polyclonal

Goat IgG

AF965

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF965

50 µg

$380

None

Polyclonal

Goat IgG

AF1071

100 µg

$355

None

Polyclonal

Goat IgG

AF1034

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1034

50 µg

$380

None

Polyclonal

Goat IgG

AF1014

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1014

50 µg

$380

None

Polyclonal

Goat IgG

AF1029

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1029

50 µg

$380

None

Polyclonal

Goat IgG

AF1294

100 µg

$355

b-Catenin in Human Kidney Cancer. b-Catenin was detected in paraffin-embedded human kidney cancer sections using anti-human/mouse/rat b-Catenin affinity-purified polyclonal antibody (Catalog # AF1329). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Cathepsin A/Lysosomal Carboxypeptidase A

Human Mouse Human

Cathepsin B Mouse Human Cathepsin C/DPPI

Mouse

Human Cathepsin D Mouse

Human Cathepsin E Mouse

Cathepsin F

Human Mouse

Biotin

Polyclonal

Goat IgG

BAF1294

50 µg

$380

None

Polyclonal

Goat IgG

AF1130

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1130

50 µg

$380

None

Polyclonal

Goat IgG

AF2075

100 µg

$355

None

237208

Mouse IgG1

MAB2075

500 µg

$325

None

Polyclonal

Goat IgG

AF2544

100 µg

$355

Polyclonal

Goat IgG

AF1013

100 µg

$355

Cathepsin F in Human Heart. Cathepsin F was detected in paraffin-embedded human heart tissue sections using anti-human Cathepsin F monoclonal antibody (Catalog # MAB2075). Tissues were stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Cathepsin H

32

Mouse

None



SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF952

100 µg

$355

None

204106

Rat IgG2B

MAB952

500 µg

$325

Human/Mouse

None

204101

Rat IgG2A

MAB9521

500 µg

$325

Mouse

None

Polyclonal

Goat IgG

AF1515

100 µg

$355

None

Polyclonal

Goat IgG

AF1174

100 µg

$355

None

Polyclonal

Goat IgG

AF1183

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1183

50 µg

$380

None

Polyclonal

Goat IgG

AF1080

100 µg

$355

None

182016

Mouse IgG1

MAB1080

500 µg

$325

None

Polyclonal

Goat IgG

AF934

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF934

50 µg

$380

None

Polyclonal

Goat IgG

AF1033

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1033

50 µg

$380

Human Cathepsin L

Cathepsin L in Mouse Ovary. Cathepsin L was detected in frozen sections of mouse ovary using anti-mouse Cathepsin L affinity-purified polyclonal antibody (Catalog # AF1515). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Cathepsin O

Human

Cathepsin S

Human

Cathepsin V

Human

Human Cathepsin X/Z/P Mouse CBP

Human/Mouse/Rat

None

257003

Mouse IgG2B

MAB2676

100 µg

$240

Ccd1/DIXDC1

Mouse

None

Polyclonal

Goat IgG

AF5599

100 µg

$355

Human/Rat

None

525222

Mouse IgG1

MAB4499

100 µg

$240

None

Polyclonal

Rabbit IgG

AF2680

50 µg

$285

None

Polyclonal

Goat IgG

AF1774

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1774

50 µg

$380

None

280702

Mouse IgG1

MAB28171

500 µg

$325

None

23002

Mouse IgG2B

MAB2791

500 µg

$325

Biotin

Polyclonal

Goat IgG

BAF270

50 µg

$380

None

93321

Mouse IgG1

MAB270

500 µg

$325

None

Polyclonal

Goat IgG

AF-450-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF-271-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF271

None

Polyclonal

Goat IgG

AF-451-NA

CCK4 aa 31-199

CCK4 in Human Colon Cancer. Colon carcinoma kinase 4 (CCK4) was detected in a paraffin-emedded section of human colon cancer using anti-human CCK4 monoclonal antibody (Catalog # MAB4499). The tissue was stained using the anti-mouse HRPDAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

CCK-A R aa 256-267

CCL2/JE/MCP-1

Human/Mouse/Rat

Canine

Human

CCL3/MIP-1a

Human Mouse Human

CCL4/MIP-1b Mouse Human CCL5/RANTES

Mouse

50 µg

$380

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF451

50 µg

$380

None

Polyclonal

Goat IgG

AF-278-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF478

100 µg

$355

None

53405

Rat IgG2A

MAB478

500 µg

$325


33


SPECIES Human

CCL11/Eotaxin Mouse

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF-320-NA

100 µg

$355

None

43911

Mouse IgG1

MAB320

500 µg

$325

None

Polyclonal

Goat IgG

AF-420-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF420

50 µg

$380

Biotin

42285

Rat IgG2A

BAM420

250 µg

$380

None

42285

Rat IgG2A

MAB420

500 µg

$325

None

Polyclonal

Goat IgG

AF802

100 µg

$355

CCL16/HCC-4

Human

None

70218

Mouse IgG1

MAB328

500 µg

$325

CCL17/TARC

Human

None

Polyclonal

Goat IgG

AF364

100 µg

$355

Human

None

54909

Mouse IgG2B

MAB361

500 µg

$325

Mouse

None

Polyclonal

Goat IgG

AF880

100 µg

$355

None

Polyclonal

Goat IgG

AF360

100 µg

$355

CCL19/MIP-3b

CCL20/MIP-3a

Human

Biotin

Polyclonal

Goat IgG

BAF360

50 µg

$380

CCL21/6Ckine

Human

None

Polyclonal

Goat IgG

AF366

100 µg

$355

CCL24/Eotaxin-2/MPIF-2

Human

None

Polyclonal

Goat IgG

AF343

100 µg

$355 $355

CCL25/TECK

Mouse

None

Polyclonal

Goat IgG

AF-481-NA

100 µg

CCL25/TECK

Mouse

None

89827

Rat IgG2A

MAB4811

500 µg

$325

CCL26/Eotaxin-3

Human

None

Polyclonal

Goat IgG

AF653

100 µg

$355

None

Polyclonal

Goat IgG

AF376

100 µg

$355

None

124308

Mouse IgG1

MAB376

500 µg

$325

None

124302

Mouse IgG2A

MAB3761

500 µg

$325

None

Polyclonal

Goat IgG

AF725

100 µg

$355

Biotin

68706

Rat IgG2A

BAM7251

250 µg

$380

None

68706

Rat IgG2A

MAB7251

500 µg

$325

Human CCL27/CTACK Mouse

CCL27/CTACK in Normal Human Skin. CCL27/ CTACK was detected in a frozen tissue section of normal human skin using anti-human CCL27/CTACK affinity-purified polyclonal antibody (Catalog # AF376). Tissues were stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue).

CCR1

Human

None

53504

Mouse IgG2B

MAB145

500 µg

$325

CCR3

Human

None

61828

Rat IgG2A

MAB155

500 µg

$325

CCR5

Human

None

45523

Mouse IgG2B

MAB181

500 µg

$325 $325

CCR6

CCR7

None

45549

Mouse IgG2B

MAB183

500 µg

Human

None

53103

Mouse IgG2B

MAB195

500 µg

$325

Mouse

None

140706

Rat IgG2A

MAB590

500 µg

$325

Human

None

150503

Mouse IgG2A

MAB197

500 µg

$325

Mouse

None

4B12

Rat IgG2A

MAB3477

100 µg

$240

None

112509

Mouse IgG2A

MAB179

500 µg

$325

None

248621

Mouse IgG2A

MAB1791

500 µg

$325

CCR7 in Human Peripheral Blood Mononuclear Cells. CCR7 was detected in human PBMCs using anti-human CCR7 monoclonal antibody (Catalog # MAB197; red). Cells were counterstained green.

CCR9

34

Human


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE CD2 CD3

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

Human

None

299812

Mouse IgG1

MAB18561

100 µg

$240

Biotin

17A2

Rat IgG2B

BAM4841

250 µg

$380

None

17A2

Rat IgG2B

MAB4841

500 µg

$325

UCHT1

Mouse IgG1

MAB100

500 µg

$325

None

Polyclonal

Goat IgG

AF2410

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2410

50 µg

$380

None

296712

Mouse IgG2B

MAB2410

100 µg

$240

None

Polyclonal

Goat IgG

AF2597

100 µg

$355

Mouse

PRICE

CD3-expressing Cells in Mouse Brain (perivascular cuffing). Cells expressing CD3 were detected in inflamed mouse brain using anti-mouse CD3 affinitypurified (Catalog # MAB4841; green). The nuclei were counterstained with DAPI (blue).

CD3e

Human

None

CD3ε in Human Peripheral Blood Mononuclear Cells (PBMCs). CD3ε was detected in human PBMCs using anti-human CD3ε monoclonal antibody (Catalog # MAB100). Cells were stained using NorthernLights-557 anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

Canine

CD4

Feline

Human

Mouse Human CD5

Mouse Feline

CD8a

372711

Mouse IgG2A

MAB25971

100 µg

$240

Polyclonal

Goat IgG

AF-379-NA

100 µg

$355

None

34930

Mouse IgG1

MAB379

500 µg

$325

Biotin

GK1.5

Rat IgG2B

BAM554

250 µg

$380

None

GK1.5

Rat IgG2B

MAB554

500 µg

$325

None

Polyclonal

Goat IgG

AF1636

100 µg

$355

Biotin

53-7.3

Rat IgG2A

BAM115

250 µg

$380

None

53-7.3

Rat IgG2A

MAB115

500 µg

$325

None

Polyclonal

Goat IgG

AF2598

100 µg

$355

Biotin

53-6.7

Rat IgG2A

BAM116

250 µg

$380

None

53-6.7

Rat IgG2A

MAB116

500 µg

$325

100 µg

$240

CD9

Mouse

None

479608

Rat IgG2A

MAB5218

CD14

Human

Biotin

Polyclonal

Sheep IgG

BAF383

50 µg

$380 $355

CD23/Fce RII

Human

None

Polyclonal

Goat IgG

AF123

100 µg

CD24

Human

None

528807

Mouse IgG2B

MAB5248

100 µg

$240

CD27/TNFRSF7

Human

Biotin

Polyclonal

Goat IgG

BAF382

50 µg

$380

CD27 Ligand/TNFSF7

Human

None

301731

Mouse IgG2B

MAB2738

100 µg

$240

CD28

Human

None

Polyclonal

Goat IgG

AF-342-PB

200 µg

$355

CD30/TNFRSF8

Mouse

None

Polyclonal

Goat IgG

AF852

100 µg

$355

CD31/PECAM-1

Human

None

Polyclonal

Sheep IgG

AF806

100 µg

$355

None

9G11

Mouse IgG1

BBA7

100 µg

$230

CD34

Rat

None

Polyclonal

Goat IgG

AF4117

100 µg

$355

CD38

Human

None

240742

Mouse IgG2A

MAB2404

500 µg

$325

Human

None

82111

Mouse IgG2B

MAB6321

500 µg

$325

Mouse

None

Polyclonal

Goat IgG

AF440

100 µg

$355

CD40/TNFRSF5

Mouse

None None


35


SPECIES Human

CD40 Ligand/TNFSF5 Mouse

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF617

100 µg

$355

None

40804

Mouse IgG2B

MAB617

500 µg

$325

None

Polyclonal

Goat IgG

AF1163

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1163

50 µg

$380

CD43

Human

None

290111

Mouse IgG2B

MAB2038

100 µg

$240

CD44 H

Human

None

2C5

Mouse IgG2A

BBA10

200 µg

$315

CD44 v3

Human

None

3G5

Mouse IgG2B

BBA11

200 µg

$315

CD44 v4/5

Human

None

3D2

Mouse IgG1

BBA25

200 µg

$315

CD44 v6

Human

None

2F10

Mouse IgG1

BBA13

200 µg

$315

Biotin

2D1

Mouse IgG1

BAM1430

100 µg

$295

None

2D1

Mouse IgG1

MAB1430

100 µg

$240

None

Polyclonal

Goat IgG

AF114

100 µg

$355

Biotin

30-F11

Rat IgG2B

BAM114

250 µg

$380

None

30-F11

Rat IgG2B

MAB114

500 µg

$325

None

Polyclonal

Goat IgG

AF2005

100 µg

$355

Human CD45 Mouse

CD46

Human

CD46 in Human Skin. CD46 was detected in paraffin-embedded human skin tissue sections using antihuman CD46 affinity-purified polyclonal antibody (Catalog # AF2005). Tissues were stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

CD47 CD47 N-terminal IgV-like Extracellular Domain

CD48/SLAMF2

Human

None

Polyclonal

Sheep IgG

AF4670

100 µg

$355

None

Polyclonal

Goat IgG

AF1866

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1866

50 µg

$380

Human

None

394620

Mouse IgG2B

MAB36441

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF3327

100 µg

$355

None

Polyclonal

Goat IgG

AF2009

100 µg

$355

Mouse

CD55/DAF

Human

CD58/LFA-3

Human

CD59

Human

Biotin

CD63

Mouse

None

CD68/SR-D1

Human

None

CD69

Human

Mouse CD72

CD83

36

Mouse

Biotin

Polyclonal

Goat IgG

BAF2009

50 µg

$380

None

Polyclonal

Goat IgG

AF1689

100 µg

$355

None

Polyclonal

Goat IgG

AF1987

100 µg

$355

Polyclonal

Goat IgG

BAF1987

50 µg

$380

446703

Rat IgG1

MAB5417

100 µg

$240

298807

Mouse IgG2B

MAB20401

100 µg

$240

None

Polyclonal

Goat IgG

AF2359

100 µg

$355

None

298633

Mouse IgG2A

MAB2359

100 µg

$240

None

298614

Mouse IgG2A

MAB23591

100 µg

$240

None

310116

Rat IgG1

MAB23861

100 µg

$240

None

Polyclonal

Goat IgG

AF1279

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1279

50 µg

$380

Human

None

HB15e

Mouse IgG1

MAB1774

500 µg

$325

Mouse

None

Polyclonal

Goat IgG

AF1437

100 µg

$355


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE CD109

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Sheep IgG

AF4385

100 µg

$355

None

455807

Rat IgG2A

MAB4609

100 µg

$240

None

Polyclonal

Goat IgG

AF2530

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2530

50 µg

$380

None

300903

Mouse IgG1

MAB2530

100 µg

$240

CD109 in Human Squamous Cell Carcinoma. CD109 was detected in paraffin-embedded human squamous cell carcinoma sections using anti-human CD109 affinity-purified polyclonal antibody (Catalog # AF4385). Tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

CD151

CD155/PVR

CD200

CD200 R1

Mouse

Human

None

300907

Mouse IgG1

MAB25301

100 µg

$240

Human

None

Polyclonal

Goat IgG

AF2724

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF3355

100 µg

$355

Human

None

380529

Mouse IgG2B

MAB34141

100 µg

$240

None

Polyclonal

Goat IgG

AF2554

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2554

50 µg

$380 $285

Mouse

Phospho-CDC2 (Y15)

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF888

100 µg

CDC25A

Human/Mouse/Rat

None

336445

Mouse IgG2B

MAB1648

100 µg

$240

CDC25B aa 377-566

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF1649

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF2666

100 µg

$355

CDCP1 CDNF

Mouse

None

Polyclonal

Sheep IgG

AF4515

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF5097

100 µg

$355

CDNF in Human Cortex. Conserved Dopamine Neurotrophic Factor (CDNF) was detected in paraffinembedded human cortex sections using anti-human CDNF affinity-purified polyclonal antibody (Catalog # AF5097). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

CDO

Human

None

Polyclonal

Sheep IgG

AF4384

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF2429

100 µg

$355

None

Polyclonal

Goat IgG

AF3665

100 µg

$355

None

Polyclonal

Goat IgG

AF2807

100 µg

$355

None

322007

Mouse IgG2B

MAB2807

100 µg

$240

None

Polyclonal

Goat IgG

AF2244

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2244

50 µg

$380

None

283324

Mouse IgG1

MAB22441

500 µg

$325

CDO Expression in Embryonic Mouse Retina. CAMrelated/Down-regulated by Oncogenes (CDO) was detected in a cryostat tissue section of embryonic mouse retina (13 d.p.c.) using anti-mouse CDO polyclonal antibody (Catalog # AF2429) and anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). Tissues were counterstained with hematoxylin (blue).

CDX2

Human

CDX4

Human

CEACAM-1/CD66a

Human


37


SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Sheep IgG

AF4128

100 µg

$355

None

487609

Mouse IgG2A

MAB41281

100 µg

$240

CEACAM-5/CD66e

Human

Cerberus 1

Human

None

162836

Mouse IgG2B

MAB1075

500 µg

$325

CFTR C-Terminus

Human

None

24-1

Mouse IgG2A

MAB25031

50 µg

$215

CFTR R Domain

Human

None

13-1

Mouse IgG1

MAB1660

50 µg

$215

Chitinase 3-like 1

Human

None

321806

Rat IgG2B

MAB2599

100 µg

$240

Chk2 aa 1-234

Mouse

None

235303

Rat IgG2A

MAB22851

100 µg

$240

Phospho-Chk2 (T68)

Human

None

Polyclonal

Rabbit IgG

AF1626

100 µg

$285

Human

None

316223

Rat IgG1

MAB2126

100 µg

$240

CHL-1/L1CAM-2

Mouse

None

Polyclonal

Goat IgG

AF2147

100 µg

$355

Choline Acetyltransferase/ChAT Isoform R

Human

None

Polyclonal

Goat IgG

AF3447

100 µg

$355

Chondrolectin

Human

None

396305

Mouse IgG2B

MAB2576

100 µg

$240

Chordin

Mouse

Chordin-Like 2/CHRDL2

Human

None

Polyclonal

Goat IgG

AF758

100 µg

$355

None

Polyclonal

Goat IgG

AF2448

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2448

50 µg

$380 $240

Mouse

None

328905

Rat IgG2A

MAB2520

100 µg

Chorionic Gonadotropin, a Chain (a HCG)

Human

None

381012

Mouse IgG2A

MAB4169

100 µg

$240

CIS-1

Human

None

Polyclonal

Goat IgG

AF3194

100 µg

$355

Claspin

Human

None

Polyclonal

Goat IgG

AF3310

100 µg

$355

Claudin-3

Human

None

385021

Mouse IgG2A

MAB4620

100 µg

$240

Claudin-12

Human

None

393314

Mouse IgG2A

MAB4284

100 µg

$240

Claudin-12 in Murine NS0 Cells. Claudin-12 was detected in a Claudin-12-transfected murine NS0 myeloma cell line using anti-human Claudin-12 monoclonal antibody (Catalog # MAB4284). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

CL-P1/COLEC12

Human

None

Polyclonal

Goat IgG

AF2690

100 µg

$355

Clusterin

Mouse

None

Polyclonal

Goat IgG

AF2747

100 µg

$355

Clusterin Isoform 1

Human

None

Polyclonal

Goat IgG

AF2937

100 µg

$355

CNP

Human

None

Polyclonal

Goat IgG

AF3127

100 µg

$355

CNP Propeptide

Human

None

347625

Rat IgG2A

MAB3739

100 µg

$240

None

Polyclonal

Total Goat IgG

AB-557-NA

1 mg

$355

None

Polyclonal

Goat IgG

AF-557-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF557

None

Polyclonal

Goat IgG

AF-559-NA

Biotin

Polyclonal

Goat IgG

Polyclonal

Goat IgG

CNTF

CNTF Ra

Rat

Rat

50 µg

$380

100 µg

$355

BAF559

50 µg

$380

AF2339

100 µg

$355

CNTF Ra in Rat Brain. Ciliary Neurotrophic Factor Receptor a (CNTF Ra) was detected in a cryostat tissue section of rat brain using biotinylated anti-rat CNTF Ra polyclonal antibody (Catalog # BAF559). Tissues were stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue). Antigen retrieval using Antigen retrieval-Basic Reagent is recommended (Catalog # CTS013).

Coagulation Factor III/Tissue Factor

38

Human

None



Coagulation Factor VII

SPECIES

Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF2338

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2338

50 µg

$380

None

321621

Mouse IgG1

MAB2338

100 µg

$240

Coagulation Factor VII in Human PBMCs. Coagulation Factor VII was detected in PHA-stimulated human peripheral blood mononuclear cells (PBMCs) using anti-human coagulation factor VII monoclonal antibody (Catalog # MAB2338; red). Cells were counterstained (green).

Collagen II

Mouse

None

Polyclonal

Sheep IgG

AF3615

100 µg

$355

Collectrin

Mouse

None

Polyclonal

Sheep IgG

AF4965

100 µg

$355

Complement Component C1qC aa 115-245

Human

None

Polyclonal

Goat IgG

AF3337

100 µg

$355

Complement Component C3d

Mouse

None

Polyclonal

Goat IgG

AF2655

100 µg

$355

Contactin-1

Human

None

Polyclonal

Goat IgG

AF904

100 µg

$355

Contactin-2/TAG1

Human

None

Polyclonal

Goat IgG

AF1714

100 µg

$355

None

372944

Mouse IgG2B

MAB1714

100 µg

$240

Corneodesmosin

Human

None

Polyclonal

Sheep IgG

AF5725

100 µg

$355

COUP-TF I/NR2F1

Human

None

H8132

Mouse IgG2A

PP-H8132-00

100 µg

$380

None

H8132

Mouse IgG2A

PP-H8132-10

100 µg

$380

None

H7147

Mouse IgG2A

PP-H7147-00

100 µg

$380

None

H7147

Mouse IgG2A

PP-H7147-10

100 µg

$380

COUP-TF II/NR2F2

Human

COX-2 aa 18-604

Human/Mouse Human

C-Reactive Protein/CRP

None

Polyclonal

Goat IgG

AF4198

100 µg

$355

None

Polyclonal

Sheep IgG

AF1707

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF1707

50 µg

$380

Mouse

None

Polyclonal

Goat IgG

AF1829

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF1744

100 µg

$355

Polyclonal

Goat IgG

AF2989

100 µg

$355

C-Reactive Protein in Human Kidney Cancer. CReactive Protein (CRP) was detected in paraffinembedded human kidney cancer tissue sections using anti-human CRP affinity-purified polyclonal antibody (Catalog # AF1707). Tissues were stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Human

None

321120

Mouse IgG1

MAB2989

100 µg

$240

CRELD1

Mouse

None

Polyclonal

Goat IgG

AF4116

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF2796

100 µg

$355 $355

CRHBP CRHR-1

Cripto

None

CREB aa 136-341

Mouse

None

Polyclonal

Goat IgG

AF3087

100 µg

Human

None

343919

Mouse IgG2A

MAB3930

100 µg

$240

None

Polyclonal

Sheep IgG

AF1538

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF1538

50 µg

$380

None

237603

Rat IgG2B

MAB1538

150 µg

$240

MAB2771

100 µg

$240

Mouse

Cripto 1

Human

None

89646

Mouse IgG1

CRISP-3

Human

None

295203

Mouse IgG1

MAB23971

100 µg

$240

CRY1

Human/Mouse

None

Polyclonal

Goat IgG

AF3764

100 µg

$355

Cryptic

Mouse

None

221428

Rat IgG2B

MAB18401

100 µg

$240

CSL

Human

None

Polyclonal

Goat IgG

AF4079

100 µg

$355


39

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE CTGF/CCN2 C-Terminus

SPECIES Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF660

100 µg

$355

None

88430

Mouse IgG1

MAB660

500 µg

$325

CTLA-4

Human

None

Polyclonal

Goat IgG

AF-386-PB

100 µg

$355

Cubilin

Human

None

Polyclonal

Sheep IgG

AF3700

100 µg

$355

None

Polyclonal

Goat IgG

AF365

100 µg

$355

None

81513

Mouse IgG1

MAB3651

500 µg

$325

None

Polyclonal

Goat IgG

AF537

100 µg

$355

CX3CL1/Fractalkine Chemokine Domain

Human Rat

CXADR

Mouse

None

Polyclonal

Goat IgG

AF2654

100 µg

$355

CXCL1/GROa/KC/CINC-1

Rat

None

Polyclonal

Goat IgG

AF-515-NA

100 µg

$355

CXCL2/GROb/MIP-2/CINC-3

Mouse

None

Polyclonal

Goat IgG

AF-452-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF452

50 µg

$380

None

Polyclonal

Goat IgG

AF254

100 µg

$355

None

33160

Mouse IgG1

MAB254

500 µg

$325

None

Polyclonal

Goat IgG

AF793

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF793

50 µg

$380

CXCL5/ENA-78

CXCL7/Thymus Chemokine-1

Human

Mouse Rat

Canine

CXCL8/IL-8

Feline

Human

CXCL8/IL-8 NorthernLights-conjugated CXCL9/MIG

Human Human Human

CXCL10/IP-10/CRG-2

CXCL12/SDF-1 CXCL13/BLC/BCA-1

Mouse

None

Polyclonal

Goat IgG

AF1116

100 µg

$355

None

Polyclonal

Goat IgG

AF1608

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1608

50 µg

$380

None

258911

Mouse IgG1

MAB1608

100 µg

$240

None

Polyclonal

Goat IgG

AF2277

100 µg

$355

None

Polyclonal

Goat IgG

AF-208-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF208

50 µg

$380

None

6217

Mouse IgG1

MAB208

500 µg

$325

NL637

6217

Mouse IgG1

NL208V

500 µL

$345

None

Polyclonal

Goat IgG

AF392

100 µg

$355

None

49106

Mouse IgG1

MAB392

500 µg

$325

None

Polyclonal

Goat IgG

AF-266-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF-466-NA

100 µg

$355 $380

Biotin

Polyclonal

Goat IgG

BAF466

50 µg

Human/Mouse

None

79018

Mouse IgG1

MAB350

500 µg

$325

Human

None

Polyclonal

Goat IgG

AF801

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF470

100 µg

$355

None

Polyclonal

Goat IgG

AF976

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF976

50 µg

$380 $240

CXCL16

Human

CXCR4

Feline

None

374606

Mouse IgG2A

MAB4287

100 µg

Human

None

44716

Mouse IgG2B

MAB172

500 µg

$325

Mouse

None

247506

Rat IgG2B

MAB21651

100 µg

$325

CXCR5

Human

None

51505

Mouse IgG2B

MAB190

500 µg

$325

CXCR6

Human

None

56811

Mouse IgG2B

MAB699

500 µg

$325

None

358426

Mouse IgG2A

MAB4227

100 µg

$240

None

11G8

Mouse IgG1

MAB42273

100 µg

$240

None

328303

Mouse IgG1

MAB4314

100 µg

$240

CXCR4

CXCR7/RDC-1

Human

Cyclin D1

Human

40


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Cyr61/CCN1

SPECIES Mouse

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Sheep IgG

AF4055

100 µg

$355

None

466305

Rat IgG2B

MAB4864

100 µg

$240

Cyr61 in Developing Embryonic Mouse Rib. Cyr61 was detected in cryostat sections of E13 mouse embryo using anti-mouse Cry61 affinity-purified polyclonal antibody (Catalog # AF4055). Tissues were stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Cystatin A Cystatin B

Human

None

Polyclonal

Goat IgG

AF1407

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF1408

100 µg

$355

Mouse

None

227807

Rat IgG2A

MAB14091

500 µg

$325

Cystatin B in Mouse Cerebellum. Cystatin B was detected in a frozen section of mouse cerebellum using anti-mouse Cystatin B monoclonal antibody (Catalog # MAB14091). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

Cystatin C

Cystatin E/M

Human

None

Polyclonal

Goat IgG

AF1196

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF1238

100 µg

$355

None

Polyclonal

Goat IgG

AF1286

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1286

50 µg

$380

None

211515

Mouse IgG2B

MAB1286

500 µg

$325

Human

Cytochrome c

Human/Equine

None

2B5F8

Mouse IgG2A

MAB898

100 µg

$240

Cytokeratin 8

Human

None

LP3K

Mouse IgG1

MAB3165

100 µg

$240

Cytokeratin 14

Human

None

LL001

Mouse IgG2A

MAB3164

100 µg

$240

Cytokeratin 19

Human

None

BA17

Mouse IgG1

MAB3506

100 µg

$240

DAN

Human

None

Polyclonal

Goat IgG

AF955

100 µg

$355

DANCE

Human

None

293904

Mouse IgG1

MAB3095

100 µg

$240

DARC

Human

None

358307

Mouse IgG2A

MAB4139

100 µg

$240

DARPP-32 aa 51-204

Mouse/Rat

375604

Rat IgG2A

MAB4230

100 µg

$240

Polyclonal

Goat IgG

AF142

100 µg

$355

DcR3/TNFRSF6B

Human

Biotin

Polyclonal

Goat IgG

BAF142

50 µg

$380

DC-SIGN

Human

None

120507

Mouse IgG2B

MAB161

500 µg

$325

DC-SIGN+DC-SIGNR

Human

None

120612

Mouse IgG2A

MAB1621

500 µg

$325

None

Polyclonal

Goat IgG

AF2378

100 µg

$355

270012

Rat IgG2A

BAM2378

100 µg

$380

DcTRAIL R1/TNFRSF23

Mouse

Biotin None

270012

Rat IgG2A

MAB2378

100 µg

$240

DDR1

Human

None

Polyclonal

Goat IgG

AF2396

100 µg

$355

DDR2

Human

None

Polyclonal

Goat IgG

AF2538

100 µg

$355

DDR2

Human

None

290814

Mouse IgG2B

MAB2538

100 µg

$240

None

290804

Mouse IgG2B

MAB25381

100 µg

$240

DEC-205/CD205

Human

None

Polyclonal

Goat IgG

AF2047

100 µg

$355

None

Polyclonal

Goat IgG

AF143

100 µg

$355

None

115402

Mouse IgG1

MAB143

500 µg

$325

None

Polyclonal

Goat IgG

AF1060

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1060

50 µg

$380

Human Decorin Mouse

None None


41

R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Dectin-1/CLEC7A

MOLECULE

Human

None

259931

Mouse IgG2B

MAB1859

100 µg

$240

b-Defensin 2/BD-2

Human

None

Polyclonal

Goat IgG

AF2758

100 µg

$355

Desert Hedgehog C-Terminus

Mouse

Desert Hedgehog N-Terminus Desmin

Mouse Human

SIZE

PRICE

None

Polyclonal

Goat IgG

AF196

100 µg

$355

None

Polyclonal

Goat IgG

AF733

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF733

50 µg

$380

None

Polyclonal

Goat IgG

AF3844

100 µg

$355

None

Polyclonal

Goat IgG

AF944

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF944

50 µg

$380

None

Polyclonal

Goat IgG

AF1720

100 µg

$355

None

216519

Mouse IgG2B

MAB1720

500 µg

$325

None

Polyclonal

Goat IgG

AF3316

100 µg

$355

Desmin in Human Heart. Desmin was detected in paraffin-embedded human heart sections using antihuman desmin affinity-purified polyclonal antibody (Catalog # AF3844). Tissue was stained using the antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Desmoglein-1 Propeptide

Human

Desmoglein-3

Human

Dishevelled-1

Human

Dishevelled-1 in Human Skeletal Muscle. Dishevelled-1 was detected in a paraffin-embedded human skeletal muscle section using anti-human dishevelled-1 affinity-purified polyclonal antibody (Catalog # AF3316). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Dishevelled-3

Human

None

Polyclonal

Sheep IgG

AF3754

100 µg

$355

None

Polyclonal

Goat IgG

AF1765

100 µg

$355

259631

Rat IgG2B

BAM1765

100 µg

$380

Dkk-1

Mouse

Biotin None

259631

Rat IgG2B

MAB1765

100 µg

$240

Dkk-2

Mouse

None

Polyclonal

Goat IgG

AF2435

100 µg

$355

Dkk-3

Human

None

Polyclonal

Goat IgG

AF1118

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1118

50 µg

$380

DLEC/CLEC4C/BDCA2

Human

None

Polyclonal

Goat IgG

AF1376

100 µg

$355

Human

None

251123

Mouse IgG2B

MAB18181

100 µg

$240

Mouse/Rat

None

Polyclonal

Sheep IgG

AF5026

100 µg

$355

Rat

None

Polyclonal

Sheep IgG

AF3970

100 µg

$355

422208

Mouse IgG2B

MAB4207

100 µg

$240

DLL1

DLL1 Expression in Mouse Embyronic Stomach. Delta-like 1 (DLL1) was detected in a cryostat tissue section of mouse E13.5 embryonic stomach using anti-rat DLL1 affinity-purified polyclonal antibody (Catalog # AF3970). Tissues were stained using NorthernLights-557-conjugated anti-sheep secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

DMC/VCC-1

42

Human

None


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE DMP-1

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Mouse

None

Polyclonal

Sheep IgG

AF4386

100 µg

$355

Polyclonal

Goat IgG

AF3646

100 µg

$355

DMP-1 in Developing Mouse Teeth. Dentin Matrix Protein 1 (DMP-1) was detected in a cryostat section of developing embryonic (E15.5) mouse teeth using anti-mouse DMP-1 affinity-purified polyclonal antibody (Catalog # AF4386). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

Human DNER

DOK4

None Biotin

Polyclonal

Goat IgG

BAF3646

50 µg

$380

None

Polyclonal

Goat IgG

AF2254

100 µg

$355

Biotin

298306

Rat IgG1

BAM2254

100 µg

$380

Mouse

None

298306

Rat IgG1

MAB2254

100 µg

$240

Human

None

Polyclonal

Sheep IgG

AF4826

100 µg

$355

Polyclonal

Goat IgG

AF3564

100 µg

$355

Mouse

DOK4 in Human Kidney. Downstream of kinase 4 (DOK4) was detected in a paraffin-embedded section of human kidney using anti-human DOK4 affinitypurified polyclonal antibody (Catalog # AF4826). Tissues were stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).

Dopa Decarboxylase/DDC

Human

None

Dopamine Transporter C-Terminus

Human/Mouse/Primate

None

Polyclonal

Rabbit IgG

PPS068

100 µL

$355

Dopamine Transporter Extracellular Loop 2

Human/Primate

None

Polyclonal

Rabbit IgG

PPS069

100 µL

$355

DPP6

Human

None

Polyclonal

Goat IgG

AF2360

100 µg

$355

DPPA4

DPPA5/ESG1

Human

None

Polyclonal

Sheep IgG

AF3674

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF3730

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF3125

100 µg

$355

None

Polyclonal

Goat IgG

AF3984

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF3984

50 µg

$380

None

Polyclonal

Goat IgG

AF1180

100 µg

$355

None

Polyclonal

Goat IgG

AF954

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF954

50 µg

$380

None

Polyclonal

Goat IgG

AF2437

100 µg

$355

None

Polyclonal

Goat IgG

AF3666

100 µg

$355

None

399212

Mouse IgG2B

MAB36661

100 µg

$240

329707

Mouse IgG1

MAB3110

100 µg

$240

Mouse Human

DPPIV/CD26

Mouse

DR3/TNFRSF25

Mouse

DSCAM Long Isoform

Human

DSCAM in Human A172 Cells. Down Syndrome Cell Adhesion Molecule (DSCAM) was detected in human A172 glioblastoma cell line using anti-human DSCAM affinity-purified polyclonal antibody (Catalog # AF3666). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

DSPG3

Human

None


43


SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

96201

Mouse IgG1

MAB859

500 µg

$325

None

Polyclonal

Goat IgG

AF759

100 µg

$355

Biotin

109646

Rat IgG1

BAM759

100 µg

$380

None

109646

Rat IgG1

MAB759

500 µg

$325

Phospho-Dynamin (S774)

Human/Mouse/Rat/Bovine/ Canine/Chicken/Primate

None

Polyclonal

Sheep IgG

PPS004

100 µL

$355

DYRK1A aa 438-571

Human/Rat

None

Polyclonal

Sheep IgG

AF5407

100 µg

$355 $355

Dtk

Mouse

SIZE

PRICE

E2F-1

Human

None

Polyclonal

Sheep IgG

AF4825

100 µg

EAR2/NR2F6

Human

None

N2025

Mouse IgG2A

PP-N2025-00

100 µg

$380

ECE-1

Human

None

Polyclonal

Goat IgG

AF1784

100 µg

$355

ECE-2

Human

None

233914

Rat IgG2A

MAB16451

100 µg

$240

ECM-1

Human

None

Polyclonal

Sheep IgG

AF3937

100 µg

$355

None

Polyclonal

Goat IgG

AF236

100 µg

$355

None

10825

Mouse IgG1

MAB236

500 µg

$325

None

10827

Mouse IgG1

MAB636

500 µg

$325

None

Polyclonal

Goat IgG

AF2028

100 µg

$355

ECM-1 in Human Colon Cancer. Extracellular matrix protein-1 (ECM-1) was detected in paraffin-embedded sections of human colon cancer using antihuman ECM-1 affinity-purified polyclonal antibody (Catalog # AF3937). Tissue was stained using antisheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Human EGF Mouse

EGF R/ErbB1

Mouse

Phospho-EGF R/ErbB1 (Y1173)

Human

Phospho-EGF R/ErbB1 (Y845)

Human

EGR1

Human

None

463004

Rat IgG2A

MAB5256

100 µg

$240

None

Polyclonal

Goat IgG

AF1280

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1280

50 µg

$380

None

Polyclonal

Rabbit IgG

AF1095

50 µg

$285

None

Polyclonal

Rabbit IgG

AF3394

50 µg

$285

None

Polyclonal

Goat IgG

AF2818

100 µg

$355

None

406913

Rat IgG2A

MAB2818

100 µg

$240

EGR1 in Human Prostate Cancer. Early growth response 1 (EGR1) was detected in a paraffin-embedded human prostate cancer tissue section using antihuman EGR1 monoclonal antibody (Catalog # MAB2818). Tissues were stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

eIF4E

Human/Mouse/Rat

None

299910

Mouse IgG2B

MAB3228

100 µg

$240

ELF3 aa 375-552

Human/Mouse

None

Polyclonal

Goat IgG

AF5787

100 µg

$355

EMAP-II

Human

None

Polyclonal

Goat IgG

AF1910

100 µg

$355

EMAP-II in Human Colon Cancer. Endothelial Monocyte-activation Polypeptide II (EMAP-II) was detected in a paraffin-embedded human colon cancer section using anti-human EMAP-II affinity-purified polyclonal antibody (Catalog # AF1910). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

44



LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

EMMPRIN

MOLECULE

Human

None

Polyclonal

Goat IgG

AF972

100 µg

$355

EMR1

Mouse

None

521204

Rat IgG2A

MAB5580

100 µg

$240

None

Polyclonal

Goat IgG

AF1097

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1097

50 µg

$380

None

Polyclonal

Goat IgG

AF1320

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1320

50 µg

$380

None

209701

Rat IgG2A

MAB1320

100 µg

$240

None

Polyclonal

Goat IgG

AF1524

100 µg

$355

None

211816

Mouse IgG2A

MAB1524

100 µg

$240

None

Polyclonal

Goat IgG

AF3534

100 µg

$355

Polyclonal

Goat IgG

AF4666

100 µg

$355

Human Endoglin/CD105 Mouse

Endoglycan/PODLX2

Human Mouse

Endoglycan in Mouse Testis. Endoglycan was detected in a cryostat section of mouse testis using antimouse Endoglycan affinity-purified polyclonal antibody (Catalog # AF3534). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Endomucin

Mouse

Endorepellin/Perlecan

Human

Endostatin

Human Mouse

EDNRB/Endothelin R Type B

None None

Polyclonal

Goat IgG

AF2364

100 µg

$355

None

Polyclonal

Goat IgG

AF1098

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1098

50 µg

$380

None

Polyclonal

Goat IgG

AF570

100 µg

$355

Human

None

Polyclonal

Sheep IgG

AF4496

100 µg

$355

Endothelin-1 C-Terminus, aa 53-73 (EDN1) and 15-22 (Mature)

Human

None

3G10

Rat IgG2B

MAB3440

100 µg

$240

Engrailed-2

Human

None

279122

Mouse IgG2B

MAB2600

100 µg

$240

Enolase 2

Human/Mouse

None

Polyclonal

Sheep IgG

AF5169

100 µg

$355

ENPP-7/Alk-SMase

Mouse

EN-RAGE/S100A12

Human

EpCAM/TROP-1

Human

Human EPCR Mouse

None

Polyclonal

Sheep IgG

AF5160

100 µg

$355

None

Polyclonal

Goat IgG

AF1052

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1052

50 µg

$380

None

158210

Mouse IgG2A

MAB960

500 µg

$325

None

Polyclonal

Goat IgG

AF2245

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2245

50 µg

$380

None

304519

Mouse IgG2B

MAB22451

100 µg

$240

None

Polyclonal

Goat IgG

AF2749

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2749

50 µg

$380

EPCR in Normal Human Skin. Endothelial Protein C Receptor (EPCR) was detected in a paraffin-embedded human skin section using anti-human EPCR monoclonal antibody (Catalog # MAB22451). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Human EphA1

Mouse

None

Polyclonal

Goat IgG

AF638

100 µg

$355

None

Polyclonal

Goat IgG

AF3034

100 µg

$355

None

404517

Rat IgG2A

MAB30341

100 µg

$240


45


SPECIES Human

EphA2 Mouse EphA3

Mouse

EphA4

Mouse

EphA5

Rat

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF3035

100 µg

$355

None

371805

Mouse IgG2A

MAB3035

100 µg

$240

None

Polyclonal

Goat IgG

AF639

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF639

50 µg

$380

None

Polyclonal

Goat IgG

AF640

100 µg

$355

None

Polyclonal

Goat IgG

AF641

100 µg

$355

None

Polyclonal

Goat IgG

AF541

100 µg

$355

None

86731

Mouse IgG1

MAB541

500 µg

$325

None

Polyclonal

Goat IgG

AF542

100 µg

$355

None

88512

Mouse IgG1

MAB5421

100 µg

$240

None

Polyclonal

Goat IgG

AF467

100 µg

$355

None

512012

Rat IgG2A

MAB467

100 µg

$240

None

512001

Rat IgG2A

MAB4671

100 µg

$240

Polyclonal

Goat IgG

AF432

100 µg

$355

EphA5 in Human U-118 MG Cells. EphA5 was detected in human U-118 MG glioblastoma cell line using anti-rat EphA5 monoclonal antibody (Catalog # MAB541). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

EphB1

EphB2

Rat

Mouse

EphB2 in Embryonic Mouse Brain. EphB2 was detected in a frozen section of E15 mouse brain using anti-mouse EphB2 affinity-purified polyclonal antibody (Catalog # AF467). Tissues were stained using NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained (green).

EphB3

Mouse Human

EphB4

Ephrin-A2

Mouse Mouse

None None

Polyclonal

Goat IgG

AF3038

100 µg

$355

None

Polyclonal

Goat IgG

AF446

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF446

50 µg

$380

None

Polyclonal

Goat IgG

AF603

100 µg

$355

Ephrin-A2 in Rat Embryonic Neurons. Ephrin-A2 was detected in rat embryonic hippocampal neurons using anti-mouse Ephrin-A2 affinity-purified polyclonal antibody (Catalog # AF603; green). Astrocytes were stained using an anti-GFAP antibody (red).

Ephrin-A5

Human

None

Polyclonal

Goat IgG

AF3743

100 µg

$355

Ephrin-B1

Mouse

None

Polyclonal

Goat IgG

AF473

100 µg

$355

Ephrin-B2

Mouse

None

Polyclonal

Goat IgG

AF496

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF496

50 µg

$380

Ephrin-B3

Human

None

Polyclonal

Goat IgG

AF395

100 µg

$355

Epigen

Mouse

None

Polyclonal

Goat IgG

AF1127

100 µg

$355

46


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Epimorphin/Syntaxin 2

SPECIES Mouse

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF2568

100 µg

$355

None

338930

Rat IgG2A

MAB2568

100 µg

$240

Epimorphin/Syntaxin 2 in Mouse Eye. Epimorphin was detected in frozen embryonic mouse eye cup sections using anti-mouse Epimorphin monoclonal antibody (Catalog # MAB2568). Tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

Epiregulin

ErbB2/Her2

Human

Human

None

Polyclonal

Goat IgG

AF1195

100 µg

$355

None

Polyclonal

Goat IgG

AF1129

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1129

50 µg

$380

None

191924

Mouse IgG2B

MAB1129

500 µg

$325

None

416711

Mouse IgG2B

MAB11291

100 µg

$240

Polyclonal

Rabbit IgG

AF4438

100 µg

$285

Phospho-ErbB2 in Human Breast Cancer. Phospho-ErbB2 was detected in paraffin-embedded human breast cancer tissue sections using anti-human phospho-ErbB2 affinity-purified polyclonal antibody (Catalog # AF1768). Tissues were stained using antirabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue)

Phospho-ErbB2/Her2 (Y1196)

Human

Phospho-ErbB2/Her2 (Y1248)

Human

ErbB3/Her3

ERK1/ERK2

Human

None None

Polyclonal

Rabbit IgG

AF1768

50 µg

$285

None

66219

Mouse IgG1

MAB348

500 µg

$325

None

526806

Mouse IgG2B

MAB3483

100 µg

$240

Mouse

None

Polyclonal

Sheep IgG

AF4518

100 µg

$355

Human/Mouse/Rat

None

216703

Mouse IgG1

MAB1576

100 µg

$240

Polyclonal

Rabbit IgG

AF1018

100 µg

$285

50 µg

$285

Phosphorylated ERK1/ERK2 Expression in Rat Brain Cortex. Phosphorylated ERK1/ERK2 was detected in cryostat tissue sections of rat brain cortex using anti-human/mouse/rat phospho-ERK1/ERK2 affinity-purified polyclonal antibody (Catalog # AF1018). Tissue was stained using the anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).

Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187)

Human/Mouse/Rat

None

ERK1

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF1575

ERK1 aa 1-28

Human

None

250603

Mouse IgG2A

MAB1940

100 µg

$240

None

Polyclonal

Rabbit IgG

AF1230

100 µg

$285

None

Polyclonal

Goat IgG

AF12301

100 µg

$355 $240

ERK2

Human/Mouse/Rat

None

191801

Mouse IgG2A

MAB1230

100 µg

ERK3 aa 344-721

Human

None

335102

Mouse IgG2B

MAB3196

100 µg

$240

ERK4 aa 1-126

Human/Mouse/Rat

None

355810

Rat IgG2A

MAB3914

100 µg

$240

ERK5/BMK1 aa 1-432

Human/Mouse

None

Polyclonal

Goat IgG

AF2848

100 µg

$355

None

H5844

Mouse IgG2A

PP-H5844-00

100 µg

$380

None

H5844

Mouse IgG2A

PP-H5844-10

100 µg

$380

ERRa/NR3B1

Human

ERRb/NR3B2

Human

None

H6705

Mouse IgG2A

PP-H6705-00

100 µg

$380

ERRg/NR3B3

Human

None

H6812

Mouse IgG2A

PP-H6812-00

100 µg

$380


47

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Erythropoietin Erythropoietin R

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

Human

None

Polyclonal

Total Rabbit IgG

AB-286-NA

1 mg

$355

None

Polyclonal

Goat IgG

AF1390

100 µg

$355

Mouse

Human ESAM Mouse

PRICE

Biotin

Polyclonal

Goat IgG

BAF1390

50 µg

$380

None

Polyclonal

Goat IgG

AF2688

100 µg

$355

None

408519

Mouse IgG2B

MAB4204

100 µg

$240

None

408522

Mouse IgG2B

MAB42041

100 µg

$240

None

Polyclonal

Goat IgG

AF2827

100 µg

$355

None

340236

Rat IgG2A

MAB28271

100 µg

$240

ESAM in Human Kidney. Endothelial Cell Adhesion Molecule (ESAM) was detected in paraffin-embedded human kidney sections using anti-human ESAM monoclonal antibody (Catalog # MAB42041). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissue was counterstained with hematoxylin (blue).

ESGP

Mouse

ERa/NR3A1

Human

EZH2

Human/Mouse Human

FABP1 Rat

FABP2

FABP3

Polyclonal

Sheep IgG

AF4580

100 µg

$355

Polyclonal

Sheep IgG

AF5715

100 µg

$355

None

H4624

Mouse IgG2A

PP-H4624-00

100 µg

$380

None

Polyclonal

Goat IgG

AF4767

100 µg

$355

None

Polyclonal

Goat IgG

AF2964

100 µg

$355

None

328607

Mouse IgG2A

MAB2964

100 µg

$240

None

Polyclonal

Goat IgG

AF1565

100 µg

$355

None

220119

Mouse IgG2B

MAB1565

100 µg

$240

Human

None

Polyclonal

Goat IgG

AF3078

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF1486

100 µg

$355

None

Polyclonal

Sheep IgG

AF1678

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF1678

50 µg

$380

None

242305

Rat IgG2A

MAB1678

100 µg

$240

Human

Human FABP4

None None

Mouse

None

Polyclonal

Goat IgG

AF3150

100 µg

$355

None

Polyclonal

Goat IgG

AF1443

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1443

50 µg

$380

None

258417

Rat IgG2A

MAB1443

100 µg

$240

None

Polyclonal

Goat IgG

AF3077

100 µg

$355

None

311215

Rat IgG2A

MAB3077

100 µg

$240

FABP4 in Mouse Mesenchymal Stem Cells. Fatty Acid Binding Protein 4 (FABP4) was detected in mouse mesenchymal stem cells using anti-mouse FABP4 affinity-purified polyclonal antibody (Catalog # AF1443). Cells were stained using anti-goat NorthernLights-557 (Catalog # NL001; red) and counterstained with DAPI (blue).

FABP5FABP5

Human Mouse

None

Polyclonal

Goat IgG

AF1476

100 µg

$355

FABP5

Mouse

Biotin

Polyclonal

Goat IgG

BAF1476

50 µg

$380

FABP6

Human

None

Polyclonal

Goat IgG

AF3880

100 µg

$355

FABP7

Human

FABP9

Mouse

48

Biotin

Polyclonal

Goat IgG

BAF3880

50 µg

$380

None

Polyclonal

Goat IgG

AF3166

100 µg

$355

None

Polyclonal

Goat IgG

AF2750

100 µg

$355

None

324926

Rat IgG2A

MAB2750

100 µg

$240


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE FAK aa 213-412

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

495919

Mouse IgG2B

MAB4467

100 µg

SIZE

PRICE $240

Human/Mouse/Rat

None

Polyclonal

Sheep IgG

AF4467

100 µg

$355

FAK in Human Hippocampus. Focal adhesion kinase 1 (FAK) was detected in paraffin-embedded human brain hippocampus sections using anti-human/ mouse/rat FAK affinity-purified polyclonal antibody (Catalog # AF4467). The tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Fibroblast Activation Protein a/FAP

Human

None

Polyclonal

Sheep IgG

AF3715

100 µg

$355

None

Polyclonal

Goat IgG

AF2267

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2267

50 µg

$380

Human

None

DX2

Mouse IgG1

MAB142

500 µg

$325

Mouse

None

Polyclonal

Goat IgG

AF435

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF2159

100 µg

$355

Feline Fas/TNFRSF6/CD95

Human Fas Ligand/TNFSF6

Polyclonal

Goat IgG

AF126

100 µg

$355

Polyclonal

Goat IgG

AF1858

100 µg

$355

None

252029

Mouse IgG2B

MAB1858

500 µg

$325 $240

FATP1

Human

None

308420

Mouse IgG2B

MAB3304

100 µg

Fcg RI/CD64

Human

None

10.1

Mouse IgG1

MAB1257

500 µg

$325

Fcg RII/RIII (CD32/CD16)

Mouse

None

Polyclonal

Goat IgG

AF1460

100 µg

$355

Fcg RIIB/CD32b

Human

None

Polyclonal

Goat IgG

AF1330

100 µg

$355

Fcg RIIIA/B (CD16)

Mouse

FCRL4/FcRH4

Human

Biotin

Polyclonal

Goat IgG

BAF2426

50 µg

$380

Fetuin A/AHSG

Human

None

Polyclonal

Goat IgG

AF1184

100 µg

$355

FGF acidic

Human

None

Polyclonal

Goat IgG

AF232

100 µg

$355

None

Polyclonal

Total Goat IgG

AB-233-NA

1 mg

$355

FGF basic

Human

None

Polyclonal

Goat IgG

AF-233-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF233

50 µg

$380

None

Polyclonal

Goat IgG

AF1206

100 µg

$355

None

254625

Mouse IgG2A

MAB1206

500 µg

$325

None

Polyclonal

Goat IgG

AF235

100 µg

$355

FGF-3

FGF-4

FGF-5

KGF/FGF-7

Rat

None None

Human

Human

Human Canine

Biotin

Polyclonal

Goat IgG

BAF1330

50 µg

$380

None

Polyclonal

Goat IgG

AF1960

100 µg

$355

None

Polyclonal

Goat IgG

AF2426

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF235

50 µg

$380

None

Polyclonal

Goat IgG

AF-237-NA

100 µg

$355

None

221903

Mouse IgG1

MAB2371

500 µg

$325

None

299026

Mouse IgG1

MAB1957

100 µg

$240 $315

Human

None

Polyclonal

Goat IgG

AF-251-NA

250 µg

FGF-8

Human/Mouse

None

47109

Mouse IgG1

MAB323

500 µg

$325

FGF-8b Isoform

Mouse

None

Polyclonal

Goat IgG

AF-423-NA

100 µg

$355

FGF-9

Human

None

Polyclonal

Goat IgG

AF-273-NA

100 µg

$355

FGF-10

Human

None

Polyclonal

Goat IgG

AF345

100 µg

$355

FGF-17

Human

None

121630

Mouse IgG2B

MAB319

500 µg

$325

FGF-19

Human

None

117601

Mouse IgG1

MAB969

500 µg

$325

None

Polyclonal

Goat IgG

AF2547

100 µg

$355

None

272317

Rat IgG2A

MAB2547

100 µg

$240

FGF-20

Human

FGF-21

Human

None

461804

Mouse IgG2B

MAB25372

100 µg

$240

FGF-23

Mouse

None

283507

Rat IgG2A

MAB26291

100 µg

$240

FGF-BP

Rat

None

Polyclonal

Goat IgG

AF1413

100 µg

$355


49


SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

FGF R2

Human

None

98739

Mouse IgG1

FGF R3 (IIIc)

Human

None

136312

Mouse IgG1

Human

None

240929

None Biotin

FGF R4

Mouse

SIZE

PRICE

MAB6843

500 µg

$325

MAB7662

500 µg

$325

Rat IgG2A

MAB6852

100 µg

$240

Polyclonal

Goat IgG

AF2265

100 µg

$355

Polyclonal

Goat IgG

BAF2265

50 µg

$380

AF1899

100 µg

$355

FGF R5/FGFRL1

Mouse

None

Polyclonal

Goat IgG

Fgr

Human

None

349331

Mouse IgG2A

MAB32071

100 µg

$240

Fgr aa 1-82

Human/Rat

None

Polyclonal

Goat IgG

AF3207

100 µg

$355

None

Polyclonal

Sheep IgG

AF1918

100 µg

$355

None

P1H11

Mouse IgG1

MAB1918

100 µg

$240

None

P1F11

Mouse IgM

MAB19181

100 µg

$240

Fibronectin

Human

Fibronectin in Human WS-1 Cells. Fibronectin was detected in WS-1 human dermal fibroblasts using anti-human Fibronectin monoclonal antibody (Catalog # MAB19181). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

FKBP38 aa 1-326 FKBP51

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF3580

100 µg

$355

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF4094

100 µg

$355

FKBP51 in Human Prostate. FK506 Binding Protein, 51 kDa molecular weight (FKBP51), was detected in paraffin-embedded human prostate sections using anti-human/mouse/rat FKBP51 affinity-purified polyclonal antibody (Catalog # AF4094). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

FKBP52

Human

None

425123

Rat IgG1

MAB4095

100 µg

$240

None

Polyclonal

Goat IgG

AF1255

100 µg

$355 $380

Follistatin-related Gene Protein/FLRG

Mouse

Biotin

Polyclonal

Goat IgG

BAF1255

50 µg

Flt-3/Flk-2

Mouse

None

113308

Rat IgG2A

MAB7681

100 µg

$240

Phospho-Flt-3/Flk-2 (Y591)

Human

None

Polyclonal

Rabbit IgG

AF368

50 µg

$285

Flt-3 Ligand

Feline

Follistatin

Human

None

Polyclonal

Goat IgG

AF2467

100 µg

$355

None

Polyclonal

Goat IgG

AF669

100 µg

$355

None

85918

Mouse IgG2A

MAB669

500 µg

$325

Human

None

Polyclonal

Goat IgG

AF1694

100 µg

$355

Mouse/Rat

None

228208

Rat IgG2B

MAB17381

100 µg

$240

FOLR1

Human

None

548908

Mouse IgG1

MAB5646

100 µg

$240

FosB/G0S3

Human

Follistatin-like 1/FSL1

FoxH1

FoxJ1

50

Human

Human

None

Polyclonal

Goat IgG

AF2214

100 µg

$355

None

Polyclonal

Sheep IgG

AF4248

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF4248

50 µg

$380

None

Polyclonal

Goat IgG

AF3619

100 µg

$355

None

407003

Rat IgG2B

MAB3619

100 µg

$240


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE FoxM1

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Sheep IgG

AF3975

100 µg

$355

FoxM1 in Human U2OS Cells. FoxM1 was detected in human osteosarcoma U2OS cells using antihuman FoxM1 affinity-purified polyclonal antibody (Catalog # AF3975). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained (green).

FoxP2 FoxP3

Human/Mouse

None

Polyclonal

Sheep IgG

AF5647

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF3240

100 µg

$355

FoxP3 in Human Tonsil. FoxP3 was detected in paraffin-embedded human tonsil sections using antihuman FoxP3 affinity-purified polyclonal antibody (Catalog # AF3240). Tissue was stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Human/Mouse Frizzled-1

Mouse

Biotin

162531

Rat IgG2A

BAM11201

100 µg

$240

None

Polyclonal

Goat IgG

AF1120

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1120

50 µg

$380

None

169310

Rat IgG2A

MAB1001-050

50 µg

$150

None

169310

Rat IgG2A

MAB1001-100

100 µg

$240

None

Polyclonal

Goat IgG

AF1001

100 µg

$355

Biotin

169310

Rat IgG2A

BAM1001

100 µg

$295

Biotin

145901

Rat IgG2A

BAM194

100 µg

$240

None

145901

Rat IgG2A

MAB194-050

50 µg

$150

None

145901

Rat IgG2A

MAB194-100

100 µg

$240

None

Polyclonal

Goat IgG

AF194

100 µg

$355

Frizzled-1 Expression in Mouse Intestine. Friz zled-1 was detected in a cryostat tissue section of embryonic mouse intestine (E13) using anti-mouse Frizzled-1 affinity-purified polyclonal antibody (Catalog # AF1120). Tissues were stained using the antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Human/Mouse Frizzled-3 Mouse

Frizzled-4

Human/Mouse

Mouse Frizzled-6

Frizzled-7

Mouse Human/Mouse

None

Polyclonal

Goat IgG

AF1526

100 µg

$355

None

151143

Rat IgG2A

MAB1981-050

50 µg

$150

None

151143

Rat IgG2A

MAB1981-100

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF198

100 µg

$355

Frizzled-8

Mouse

None

Polyclonal

Goat IgG

AF112

100 µg

$355

Frizzled-9

Mouse

None

Polyclonal

Goat IgG

AF2440

100 µg

$355


51

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Frk aa 1-107

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

393812

Rat IgG1

MAB3766

100 µg

SIZE

PRICE $240

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF3766

100 µg

$355

Frk in Human Breast Cancer. Fyn-related kinase (Frk) was detected in paraffin-embedded human breast cancer sections using anti-human Frk monoclonal antibody (Catalog # MAB3766). Tissue was subjected to antigen retrieval using Antigen Retrieval Kit (Catalog # CTS013; brown). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

FRS2

Human

None

Polyclonal

Sheep IgG

AF4069

100 µg

$355

Fucosyltransferase 8/FUT8

Human

None

Polyclonal

Sheep IgG

AF5768

100 µg

$355

Furin

Human

None

222728

Mouse IgG1

MAB15031

500 µg

$325

FXR/NR1H4

Human

None

A9033A

Mouse IgG2A

PP-A9033A-00

100 µg

$380

Fyn

Human

None

Polyclonal

Goat IgG

AF3574

100 µg

$355

G9a/EHMT2

Human/Mouse

None

A8620A

Mouse IgG2A

PP-A8620A-00

100 µg

$380

GABAA R d N-Terminus

Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS090

100 µL

$355

GABAB R2 N-Terminus

Rat

None

Polyclonal

Goat IgG

AF1188

100 µg

$355

GAD1/GAD67 aa 2-97

Human

None

Polyclonal

Goat IgG

AF2086

100 µg

$355

None

252910

Mouse IgG1

MAB2086

100 µg

$240

GAD2/GAD65 aa 2-150

Human

None

Polyclonal

Goat IgG

AF2247

100 µg

$355

Human

Galectin-1

Mouse

Human Galectin-3 Mouse

Galectin-4

None

Polyclonal

Goat IgG

AF1152

100 µg

$355

None

Polyclonal

Goat IgG

AF1245

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1245

50 µg

$380

Biotin

201002

Rat IgG2B

BAM12451

250 µg

$380

None

201002

Rat IgG2B

MAB12451

500 µg

$325

None

Polyclonal

Goat IgG

AF1154

100 µg

$355

None

194804

Mouse IgG2B

MAB1154

500 µg

$325

None

Polyclonal

Goat IgG

AF1197

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1197

50 µg

$380

Human

None

Polyclonal

Goat IgG

AF1227

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF2128

100 µg

$355

None

Polyclonal

Goat IgG

AF1339

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1339

50 µg

$380

None

Polyclonal

Goat IgG

AF1304

100 µg

$355

None

212923

Rat IgG2A

MAB1304

500 µg

$325

None

Polyclonal

Goat IgG

AF1305

100 µg

$355

None

Polyclonal

Goat IgG

AF2045

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2045

50 µg

$380

Human Galectin-7 Mouse Galectin-8

Human

Galectin-9

Human

Galectin-9 Short Isoform

Mouse

None

Polyclonal

Goat IgG

AF3535

100 µg

$355

Gas1

Mouse

None

Polyclonal

Goat IgG

AF2644

100 µg

$355

Gas6

Human

None

Polyclonal

Goat IgG

AF885

100 µg

$355

GASP-1/WFIKKNRP

Human

None

288518

Mouse IgG2B

MAB20701

100 µg

$240

GASP-2/WFIKKN

Human

None

289802

Mouse IgG2B

MAB21363

100 µg

$240

GATA-1

Human

None

234732

Rat IgG2B

MAB1779

100 µg

$240

GATA-2

Human

None

Polyclonal

Goat IgG

AF2046

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2046

50 µg

$380

GATA-3

Human

None

291106

Mouse IgG1

MAB26051

100 µg

$240

GATA-3 aa 135-258

Human

None

Polyclonal

Goat IgG

AF2605

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2605

50 µg

$380

52


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE GATA-4

SPECIES Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF2606

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2606

50 µg

$380

GATA-4 in Differentiated Human Embryonic Stem Cells. GATA-4 was detected in differentiated human Embryonic Stem cells using anti-human GATA-4 polyclonal antibody (Catalog # AF2606; green). The cells were counterstained with DAPI (blue). Courtesy of Dr. Frank Solder from the National Institute of Neurological Disorders and Stroke & Stem Cell Unit at NIH.

GATA-5

Human

None

Polyclonal

Goat IgG

AF2170

100 µg

$355

None

Polyclonal

Goat IgG

AF1700

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1700

50 µg

$380

GATA-6

Human

None

222228

Mouse IgG1

MAB1700

100 µg

$240

GCNF/NR6A1

Human

None

Polyclonal

Goat IgG

AF3180

100 µg

$355

GDF-3

Mouse

None

Polyclonal

Goat IgG

AF958

100 µg

$355

None

Polyclonal

Goat IgG

AF853

100 µg

$355

GDF-5

Mouse

Biotin

Polyclonal

Goat IgG

BAF853

50 µg

$380

Biotin

90509

Rat IgG2A

BAM853

50 µg

$380

None

Polyclonal

Goat IgG

AF788

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF788

50 µg

$380

None

Polyclonal

Goat IgG

AF739

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF739

50 µg

$380

GDF-8/Myostatin

Mouse

GDF-9

Mouse

GDNF

Human/Rat

GFAP

Human

None

Polyclonal

Goat IgG

AF-212-NA

100 µg

$355

None

Polyclonal

Sheep IgG

AF2594

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF2594

50 µg

$380

None

273807

Mouse IgG1

MAB2594

100 µg

$240

Polyclonal

Goat IgG

AF3540

100 µg

$355

GFAP in Rat Astrocytes. Glial fibrillary acidic protein (GFAP) was detected in astrocytes in differentia ting cultures of rat cortical stem cells using antihuman GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The cells were stained using NorthernLights-557 anti-sheep secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

GFI-1

Human Human

GFRa-1/GDNF Ra-1

Rat

None None

Polyclonal

Goat IgG

AF714

100 µg

$355

None

Polyclonal

Goat IgG

AF560

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF560

50 µg

$380

None

81401

Mouse IgG2B

MAB560

500 µg

$325

GFRa-1 and GDNF Expression in Rat Dorsal Root Ganglia. GFRa-1 and GDNF were detected in rat dorsal root ganglia using biotinylated anti-rat GFRa-1 affinity-purified polyclonal antibody (Catalog # BAF560; red) and anti-human GDNF affinitypurified polyclonal antibody (Catalog # AF-212-NA; green).

GFRa-2/GDNF Ra-2

Human

None

Polyclonal

Goat IgG

AF613

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF429

100 µg

$355


53


GFRa-3/GDNF Ra-3

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF670

100 µg

$355

None

Polyclonal

Goat IgG

AF2645

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2645

50 µg

$380

Mouse

SIZE

PRICE

GFRa-3 in Mouse Embryonic Dorsal Root Ganglion (DRG). Detection of Glial cell line-derived Factor Receptor alpha 3 (GFRa-3) in a cryostat section of E15 DRG mouse using anti-mouse GFRa-3 affinity-purified polyclonal antibody (Catalog # AF2645). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS008 and counterstained with hematoxylin (blue).

None

Polyclonal

Goat IgG

AF1677

100 µg

$355

GFRa-4/GDNF Ra-4

Mouse

Biotin

Polyclonal

Goat IgG

BAF1677

50 µg

$380

None

171217

Rat IgG2A

MAB1677

500 µg

$325

GITR/TNFRSF18

Human

None

Polyclonal

Goat IgG

AF689

100 µg

$355

GLI-1

Human/Mouse

None

388516

Rat IgG2A

MAB3324

100 µg

$240

GLI-1 aa 1-234

Human

None

Polyclonal

Goat IgG

AF3324

100 µg

$355

GLI-1 aa 1-237

Human/Mouse

None

Polyclonal

Goat IgG

AF3455

100 µg

$355

GLI-2

Human

None

Polyclonal

Sheep IgG

AF3526

100 µg

$355

GLI-2 aa 1-416

Mouse

Biotin

Polyclonal

Sheep IgG

BAF3526

50 µg

$380

None

Polyclonal

Goat IgG

AF3635

100 µg

$355

GLI-3 aa 1-479

Human/Mouse

None

Polyclonal

Goat IgG

AF3690

100 µg

$355

GLP/EHMT1

Human/Mouse

None

B0422

Mouse IgG2A

PP-B0422-00

100 µg

$380

GLP-1R

Human

None

419208

Mouse IgG2A

MAB28141

100 µg

$240

GLP-2R

Human

None

413801

Mouse IgG2A

MAB4285

100 µg

$240

Glucagon

Human/Mouse

None

181402

Mouse IgG2A

MAB1249

100 µg

$240

Glucagon in bTC6 Cells. Glucagon expression was detected in bTC6 mouse insulinoma cells using antihuman/mouse Glucagon monoclonal antibody (Catalog # MAB1249). The cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody ( Catalog # NL007; red) and counterstained with DAPI (blue).

Glucagon NorthernLights-conjugated

Human/Mouse

NL557

181402

Mouse IgG2A

NL1249R

500 µL

$345

Glut1

Human

None

202915

Mouse IgG2B

MAB1418

200 µg

$270

Human

None

199017

Mouse IgG2A

MAB1414

200 µg

$270

Biotin

205115

Rat IgG2B

BAM1440

200 µg

$380

None

205115

Rat IgG2B

MAB1440

200 µg

$270

Biotin

202017

Mouse IgG2B

BAM1415

200 µg

$315

Glut2

Glut3

Mouse

Human

Glut4

Rat

Glut5

Human

54

None

202017

Mouse IgG2B

MAB1415

200 µg

$270

Biotin

1F8

Mouse IgG1

BAM1262

250 µg

$380

None

1F8

Mouse IgG1

MAB1262

200 µg

$270

Biotin

195205

Mouse IgG2A

BAM1349

200 µg

$380

None

195205

Mouse IgG2A

MAB1349

200 µg

$270


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Glutathione Peroxidase 1

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF3798

100 µg

$355

Polyclonal

Goat IgG

AF4199

100 µg

$355

GPX1 in Rat Liver. Glutathione Peroxidase 1 (GPX1) was detected in a cryostat section of rat liver using anti-human/mouse/rat GPX1 affinity-purified polyclonal antibody (Catalog # AF3798). The tissue was stained using the anti-goat Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Glutathione Peroxidase 3 aa 74-226

Human/Mouse/Rat

None

GPX3 in Human Kidney. Glutathione Peroxidase 3 (GPX3) was detected in paraffin-embedded human kidney sections using anti-human GPX3 affinity-purified polyclonal antibody (Catalog # AF4199). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Glycine R Pan Specific Glycophorin A Glypican 1

Glypican 2

Rat Human

Polyclonal

Rabbit IgG

PPS009

200 µg

$355

R10

Mouse IgG1

BAM12281

250 µg

$380

None

R10

Mouse IgG1

MAB1228

500 µg

$325

Human

None

Polyclonal

Goat IgG

AF4519

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF2304

100 µg

$355

None

Polyclonal

Goat IgG

AF2355

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2355

50 µg

$380

None

300736

Rat IgG2A

MAB2355

100 µg

$240

None

Polyclonal

Sheep IgG

AF2119

100 µg

$355

Mouse

Glypican 3

Human

Glypican 6

Mouse

GM-CSF

None Biotin

Canine

None

Polyclonal

Goat IgG

AF1053

100 µg

$355

None

Polyclonal

Goat IgG

AF1546

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1546

50 µg

$380

Human

None

3209

Mouse IgG1

MAB215

500 µg

$325

GMF-b

Human

None

Polyclonal

Goat IgG

AF1276

100 µg

$355

Goosecoid

Human

None

Polyclonal

Goat IgG

AF4086

100 µg

$355

gp130

Mouse

Biotin

125623

Rat IgG2A

BAM4681

100 µg

$240

None

125623

Rat IgG2A

MAB4681

100 µg

$240

419859

Mouse IgG2A

MAB4617

100 µg

$240

gp130 in Mouse Splenocytes. gp130 was detected in mouse splenocytes using anti-mouse gp130 monoclonal antibody (Catalog # MAB4681; red). Cells were counterstained with DAPI (blue).

GPR34

Human

None


55

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE GPR50

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

461129

Mouse IgG2A

MAB4645

100 µg

$240

None

481906

Mouse IgG2A

MAB5239

100 µg

$240

None

H8004

Mouse IgG2A

PP-H8004-00

100 µg

$380

None

H8031

Mouse IgG2A

PP-H8031-00

100 µg

$380

Biotin

RB6-8C5

Rat IgG2B

BAM1037

250 µg

$380

GPR50 in Human Glioblastoma Cells. G proteincoupled receptor 50 (GPR50) was detected in human A172 glioblastoma cell line using anti-human GPR50 monoclonal antibody (Catalog # MAB4645). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

GPRC5A

Human

GR/NR3C1

Human

Gr-1/Ly-6G

Mouse

Granzyme A

Human

None

RB6-8C5

Rat IgG2B

MAB1037

500 µg

$325

None

356412

Mouse IgG2B

MAB29051

100 µg

$240

None

351927

Mouse IgG2A

MAB2906

100 µg

$240

None

Polyclonal

Goat IgG

AF1865

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1865

50 µg

$380

None

Polyclonal

Goat IgG

AF1365

100 µg

$355

None

Polyclonal

Goat IgG

AF1377

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1377

50 µg

$380

None

Polyclonal

Goat IgG

AF3846

100 µg

$355

None

Polyclonal

Goat IgG

AF956

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF956

50 µg

$380

None

Polyclonal

Sheep IgG

AF4339

100 µg

$355

None

Polyclonal

Goat IgG

AF1067

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1067

50 µg

$380

None

Polyclonal

Goat IgG

AF1566

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1566

50 µg

$380

None

222517

Mouse IgG2B

MAB15661

500 µg

$325

Granzyme A in Human Peripheral Blood Mononuclear Cells. Granzyme A was detected in human peripheral blood mononuclear cells using antihuman Granzyme A monoclonal antibody (Catalog # MAB29051). Cells were stained using donkey antimouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and counterstained green.

Human Granzyme B

Mouse

Granzyme D

Mouse

Granzyme H

Human

GRB2

Human

GRB2 in Human Breast Cancer. Growth Factor Receptor Bound Protein 2 (GRB2) was detected in paraffin-embedded human breast cancer sections using anti-human/mouse/rat GRB2 affinity-purified polyclonal antibody (Catalog # AF3846). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). Tissue was counterstained with hematoxylin (blue).

Gremlin

Mouse

GRK2 aa 468-689

Human/Mouse/Rat Human

Growth Hormone Rat

56



SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF1210

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1210

50 µg

$380

None

Polyclonal

Goat IgG

AF1360

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1360

50 µg

$380

Rat

None

Polyclonal

Goat IgG

AF1211

100 µg

$355

Human

None

419605

Mouse IgG1

MAB35841

100 µg

$240

Human Growth Hormone R/GHR

GRP75/HSPA9B

Mouse

GRP75 in Human Fibroblastic Meningioma. Glucose Regulated 75 kDa Protein (GRP75) was detected in a paraffin-embedded human fibroblastic meningioma section using anti-human GRP75 monoclonal antibody (Catalog # MAB35841). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

GRP75/HSPA9B aa 542-679

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF3584

100 µg

$355

GSK-3a aa 90-483

Human

None

288901

Mouse IgG2B

MAB2986

100 µg

$240

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF4125

100 µg

$285

Phospho-GSK-3a (S21)

Phosphorylated GSK-3a in Human Thymus. Phosphorylated Glycogen Synthase kinase-3a (GSK-3a) was detected in paraffin-embedded human thymus tissue sections using anti-human/mouse/rat Phospho-GSK-3a (S27) affinity-purified polyclonal antibody (Catalog # AF4125). Tissues were stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).

Phospho-GSK-3a/b (S21/S9)

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF1590

100 µg

$285

GSK-3b

Human

None

272535

Rat IgG2B

MAB25061

100 µg

$240

HAI-1 aa 30-433

Mouse

None

Polyclonal

Goat IgG

AF1141

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1141

50 µg

$380

None

Polyclonal

Goat IgG

AF1048

100 µg

$355

HAI-1 Ectodomain

HAI-2 Ectodomain

Mouse

Hairless

Human

HAND1

Human

HAND2

Human/Mouse

Biotin

Polyclonal

Goat IgG

BAF1048

50 µg

$380

None

Polyclonal

Goat IgG

AF1107

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1107

50 µg

$380

None

Polyclonal

Goat IgG

AF5708

100 µg

$355

None

Polyclonal

Goat IgG

AF3168

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF3168

50 µg

$380

None

Polyclonal

Goat IgG

AF3876

100 µg

$355

HAPLN4

Human

None

Polyclonal

Goat IgG

AF4085

100 µg

$355

HB-EGF

Human

None

Polyclonal

Goat IgG

AF-259-NA

250 µg

$355

HCRTR2

Human

None

456738

Mouse IgG2A

MAB5246

100 µg

$240

Hepassocin

Rat

None

456723

Mouse IgG2B

MAB52461

100 µg

$240

None

Polyclonal

Goat IgG

AF1105

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1105

50 µg

$380

HES-1 aa 93-280

Human

None

Polyclonal

Goat IgG

AF3317

100 µg

$355

HES-4 aa 94-221

Human

None

Polyclonal

Sheep IgG

AF3600

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF-294-NA

100 µg

$355

HGF HGF Activator

Human

Mouse

None

Polyclonal

Goat IgG

AF2207

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF1514

100 µg

$355


57


SPECIES Human

HGF R/c-MET Mouse Phospho-HGF R/c-MET (Y1003)

Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF276

100 µg

$355

None

95308

Mouse IgG1

MAB3583

100 µg

$240

None

Polyclonal

Goat IgG

AF527

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF527

50 µg

$380

None

Polyclonal

Rabbit IgG

AF4059

100 µg

$285

Phospho-HGF R/c-MET (Y1234/Y1235)

Human

None

Polyclonal

Rabbit IgG

AF2480

50 µg

$285

Phospho-HGF R/c-MET (Y1349)

Human

None

Polyclonal

Rabbit IgG

AF3950

50 µg

$285

HIF-1a aa 575-826

Human/Mouse

None

241812

Mouse IgG1

MAB1935

100 µg

$240

HIF-2a aa 542-874

Mouse

None

Polyclonal

Goat IgG

AF2997

100 µg

$355

Phospho-Histone H2AX (S139)

Human

None

Polyclonal

Rabbit IgG

AF2288

50 µg

$285

HMGA2

Human

None

Polyclonal

Goat IgG

AF3184

100 µg

$355

HNF-3b/FoxA2 aa 242-457

Human

None

Polyclonal

Goat IgG

AF2400

100 µg

$355

None

H1415

Mouse IgG2A

PP-H1415-00

100 µg

$380

None

K9218

Mouse IgG2A

PP-K9218-00

100 µg

$380

PP-N3224-00

100 µg

$380

HNF-3b in Human BG01V Cells. Hepatic Nuclear Factor 3 b (HNF-3b) was detected in endoderm differentiated human BG01V embryonic stem cell line using anti-human HNF-3b affinity-purified polyclonal antibody (Catalog # AF2400). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

HNF-4a/NR2A1

Human

HNF-4g/NR2A2

Human

None

N3224

Mouse IgG2A

HNF-4a/NR2A1 Isoform 7-9

Human

None

H6939

Mouse IgG1

PP-H6939-00

100 µg

$380

HOXA1

Human

None

Polyclonal

Goat IgG

AF5014

100 µg

$355

HSP60

Human/Mouse/Rat

None

264233

Mouse IgG2B

MAB1800

100 µg

$240

HSP70

Human/Mouse

None

242707

Mouse IgG2A

MAB1663

100 µg

$240

HSPH1 aa 595-733

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF4029

100 µg

$355

229926

Mouse IgG2B

MAB1458

100 µg

$240 $285

HSPH1 in Rat Cerebellum. HSP (Heat Shock Protein) H1 was detected in a cryostat section of rat cerebellum using anti-human/mouse/rat HSPHI affinitypurified polyclonal antibody (Catalog # AF4029). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

HTRA2/Omi

Human

None

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF1458

100 µg

HVEM/TNFRSF14

Human

None

94804

Mouse IgG1

MAB3561

500 µg

$325

cIAP-1/HIAP-2

Human

None

Polyclonal

Goat IgG

AF8181

100 µg

$355

cIAP-2/HIAP-1

Human

None

Polyclonal

Goat IgG

AF8171

100 µg

$355

None

315304

Mouse IgG1

MAB817

100 µg

$240

ICA1

Human

None

Polyclonal

Goat IgG

AF4084

100 µg

$355

None

Polyclonal

Goat Serum

BBA17

500 µL

$195

None

BBIG-I1

Mouse IgG1

BBA3

100 µg

$230

None

BBIG-I1

Mouse IgG1

BBA4

1 mg

$840

None

Polyclonal

Goat IgG

AF796

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF796

50 µg

$380

None

Polyclonal

Goat IgG

AF583

100 µg

$355

Human ICAM-1/CD54 Mouse Rat

58



SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF774

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF774

50 µg

$380 $315

ICAM-2/CD102

Mouse

ICAM-3/CD50

Human

None

ICAM-3.3

Mouse IgG1

BBA15

200 µg

ICAM-5

Human

None

283607

Mouse IgG2A

MAB1950

100 µg

$240

None

Polyclonal

Goat IgG

AF1173

100 µg

$355

ICAM-5

Mouse

ICOS

Human

Biotin

Polyclonal

Goat IgG

BAF1173

None

Polyclonal

Goat IgG

AF169

50 µg

$380

100 µg

$355

ID2

Human

None

Polyclonal

Goat IgG

AF4660

100 µg

$355

IFITM1

Human

None

Polyclonal

Goat IgG

AF4827

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF3377

100 µg

$355

None

MMHA-3

Mouse IgG1

21101-1

50 µg

$195

None

MMHA-1

Mouse IgG1

21105-1

50 µg

$195

None

MMHA-8

Mouse IgG2B

21110-1

50 µg

$195

None

MMHA-11

Mouse IgG1

21112-1

50 µg

$250

Fluorescein

MMHA-11

Mouse IgG1

21112-3

50 Tests

$300

None

MMHA-13

Mouse IgG1

21116-1

50 µg

$195

None

MMHA-17

Mouse IgG1

21118-1

50 µg

$195

None

MMHA-6

Mouse IgG1

21125-1

50 µg

$195

None

MMHA-9

Mouse IgG1

21127-1

50 µg

$195

None

MMHA-14

Mouse IgG1

21129-1

50 µg

$195

None

Polyclonal

Sheep Serum

31100-1

100000 U

$235

None

Polyclonal

Rabbit Serum

31101-1

100000 U

$235

None

Polyclonal

Rabbit Serum

31130-1

20000 U

$215

Fluorescein

RMMA-1

Rat IgG1

22100-3

100 µL

$355

IFITM3/Fragilis

IFITM3/Fragilis in Embryonic Mouse Ovary. Interferon-induced transmembrane protein 3 (IFITM3) was detected in an E13.5 mouse embryonic ovary section using anti-mouse IFITM3 affinity-purified polyclonal antibody (Catalog # AF3377). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Human

IFN-a

Mouse

Porcine

None

Polyclonal

Rabbit Serum

32100-1

20000 U

$325

None

K9

Mouse IgG1

27100-1

500 µg

$315

None

F17

Mouse IgG1

27105-1

500 µg

$315

IFN-a/b R1

Mouse

Biotin

Polyclonal

Goat IgG

BAF3039

50 µg

$380

Phospho-IFN-a/b R1 (S535/S539)

Human/Mouse/Rat/Bovine/ Canine/Primate/Sheep

None

Polyclonal

Rabbit IgG

PPS074

100 µL

$355

IFN-a/b R2

Human

None

MMHAR-2

Mouse IgG2A

21385-1

50 µg

$330

None

Polyclonal

Rabbit Serum

31385-1

50 µg

$250


59


SPECIES

Human IFN-b

Mouse

Bovine

Canine Equine IFN-g Human

Mouse

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Fluorescein

MMHB-3

Mouse IgG1

21400-3

50 Tests

$270

None

MMHB-1

Mouse IgG2A

21405-1

50 µg

$195

None

MMHB-12

Mouse IgG1

21450-1

50 µg

$195

None

MMHB-13

Mouse IgG3

21455-1

50 µg

$195

None

MMHB-14

Mouse IgG2A

21460-1

50 µg

$195

None

MMHB-15

Mouse IgG2B

21465-1

50 µg

$195

None

MMHB-16

Mouse IgG1

21470-1

50 µg

$195

None

Polyclonal

Sheep Serum

31400-1

20000 U

$280

None

Polyclonal

Rabbit Serum

31410-1

20000 U

$225

Fluorescein

RMMB-1

Rat IgG1

22400-3

100 µL

$320

None

Polyclonal

Rabbit Serum

32400-1

20000 U

$315

None

Polyclonal

Goat IgG

AF2300

100 µg

$355

None

345025

Rat IgG2A

MAB2300

100 µg

$240

None

Polyclonal

Goat IgG

AF781

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF781

50 µg

$380

None

Polyclonal

Goat IgG

AF1586

100 µg

$355

None

Polyclonal

Goat IgG

AF-285-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF285

50 µg

$380

None

25718

Mouse IgG2A

MAB285

500 µg

$325

None

25723

Mouse IgG2B

MAB2851

500 µg

$325

None

H22

Hamster IgG

MAB4851

500 µg

$325

Porcine

Biotin

Polyclonal

Goat IgG

BAF985

50 µg

$380

Rat

None

Polyclonal

Goat IgG

AF-585-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF673

100 µg

$355

NL557

Polyclonal

Donkey IgG

NL001

500 µL

$115

NL637

Polyclonal

Donkey IgG

NL002

500 µL

$115

NL493

Polyclonal

Donkey IgG

NL003

500 µL

$115

NL557

Polyclonal

Donkey IgG

NL007

500 µL

$115

NL637

Polyclonal

Donkey IgG

NL008

500 µL

$115

NL493

Polyclonal

Donkey IgG

NL009

500 µL

$115

NL557

Polyclonal

Donkey IgG

NL004

500 µL

$115

NL637

Polyclonal

Donkey IgG

NL005

500 µL

$115

NL493

Polyclonal

Donkey IgG

NL006

500 µL

$115

NL557

Polyclonal

Goat IgG

NL013

500 µL

$115

NL637

Polyclonal

Goat IgG

NL014

500 µL

$115

NL493

Polyclonal

Goat IgG

NL015

500 µL

$115

NL557

Polyclonal

Donkey IgG

NL010

500 µL

$115

NL637

Polyclonal

Donkey IgG

NL011

500 µL

$115

NL493

Polyclonal

Donkey IgG

NL012

500 µL

$115

IFN-g in Human Peripheral Blood Mononuclear Cells (PBMC). Interferon g (IFN-g) was detected in PMA-, ionomyocin-, and monensin-activated human PBMC using anti-human IFN-g affinity-purified polyclonal antibody (Catalog # AF-285-NA). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

IFN-g R1/CD119

Human

Goat

Mouse

IgG NorthernLights-conjugated

Rabbit

Rat

Sheep

IgG2A

Mouse

None

133304

Mouse IgG2A

MAB0031

500 µg

$170

IgG2B

Mouse

None

73009

Mouse IgG2B

MAB0042

500 µg

$170

60



SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

NL557

Polyclonal

Goat IgG

NL016

500 µL

$115

Polyclonal

Goat IgG

NL017

500 µL

$115

IgY NorthernLights-conjugated

Chicken

NL637 NL493

Polyclonal

Goat IgG

NL018

500 µL

$115

IGFBP-1

Mouse

None

Polyclonal

Goat IgG

AF1240

100 µg

$355

IGFBP-3

Human

None

Polyclonal

Goat IgG

AF675

100 µg

$355

None

84728

Mouse IgG2B

MAB305

500 µg

$325

None

Polyclonal

Goat IgG

AF804

100 µg

$355

IGFBP-4

IGFBP-5

Human

Human Mouse

Biotin

Polyclonal

Goat IgG

BAF804

50 µg

$380

None

Polyclonal

Goat IgG

AF875

100 µg

$355

None

164503

Mouse IgG2B

MAB875

500 µg

$325

None

Polyclonal

Goat IgG

AF578

100 µg

$355

IGFBP-5 in Mouse Ovary. Insulin-like Growth Factor Binding Protein 5 (IGFBP-5) was detected in a cryostat section of mouse ovary (follicle with oocyte) using anti-mouse IGFBP-5 affinity-purified polyclonal antibody (Catalog # AF578). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

IGFBP-6 IGFBP-L1

IGFBP-rp1/IGFBP-7

IGF-I IGF-I R IGF-II

Mouse Mouse

Human

Polyclonal

Goat IgG

AF776

100 µg

$355

Polyclonal

Goat IgG

AF4130

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF4130

50 µg

$380

None

Polyclonal

Goat IgG

AF1334

100 µg

$355

50 µg

$380

Biotin

Polyclonal

Goat IgG

BAF1334

Mouse

None

292917

Rat IgG1

MAB21201

100 µg

$240

Human

None

Polyclonal

Goat IgG

AF-291-NA

250 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF791

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF-305-NA

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF-292-NA

250 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF792

100 µg

$355

IGF-II R

Human

None

Polyclonal

Goat IgG

AF2447

100 µg

$355

IGSF2/CD101

Mouse

Biotin

307707

Rat IgG2A

BAM3368

100 µg

$380

IGSF4B

Human

None

Polyclonal

Goat IgG

AF3678

100 µg

$355

None

404619

Rat IgG1

MAB4164

100 µg

$240

None

404617

Rat IgG1

MAB41641

100 µg

$240

None

Polyclonal

Goat IgG

AF3117

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF3117

50 µg

$380

IGSF4C

Human

IGSF8

Mouse

Ikaros

Human

None

Polyclonal

Goat IgG

AF4984

100 µg

$355

IKKa aa 375-745

Human/Mouse/Rat

None

Polyclonal

Sheep IgG

AF3768

100 µg

$355

IKKg IKKe

IL-1a/IL-1F1

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF2684

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF3199

100 µg

$355

Human/Mouse/Rat

None

346909

Mouse IgG1

MAB3199

100 µg

$240

None

Polyclonal

Goat IgG

AF-200-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF200

50 µg

$380

None

4414

Mouse IgG2A

MAB200

500 µg

$325

None

Polyclonal

Goat IgG

AF-400-NA

100 µg

$355

Human

Mouse

None None


61


SPECIES Feline

Human IL-1b/IL-1F2

Mouse

Rat

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF1796

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1796

50 µg

$380

None

Polyclonal

Total Goat IgG

AB-201-NA

1 mg

$315

None

Polyclonal

Goat IgG

AF-201-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF201

50 µg

$380

None

8516

Mouse IgG1

MAB201

500 µg

$325

None

2805

Mouse IgG1

MAB601

500 µg

$325

None

Polyclonal

Goat IgG

AF-401-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF-501-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF501

50 µg

$380

None

38139

Mouse IgG1

MAB501

500 µg

$325

IL-1b in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-1b (IL-1b) was detected in LPS-stimulated human PBMCs using anti-human IL-1b affinity-purified polyclonal antibody (Catalog # AF-201-NA). The cells were stained using NorthernLights-557 anti-goat secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

IL-1b/IL-1F2 NorthernLights-conjugated

IL-1ra/IL-1F3

IL-1F5/FIL1d

Human

NL557

Polyclonal

Goat IgG

NL201R

500 µL

$350

Equine

None

Polyclonal

Goat IgG

AF2466

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF-280-NA

100 µg

$355

Porcine

None

Polyclonal

Goat IgG

AF780

100 µg

$355

Human

IL-1F6/FIL1e

Human

IL-1F8/FIL1η

Human

IL-1 RI

Mouse

IL-1 RII

IL-1b in Rat Splenocytes. Interleukin 1b (IL-1b) was detected in LPS-stimulated rat splenocytes using anti-rat IL-1b affinity-purified polyclonal antibody (Catalog # AF-501-NA). Cells were stained using NorthernLights-557 anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained (green).

Human Mouse

None

Polyclonal

Goat IgG

AF1275

100 µg

$355

None

Polyclonal

Goat IgG

AF1078

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1078

50 µg

$380

None

Polyclonal

Goat IgG

AF1099

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1099

50 µg

$380

None

Polyclonal

Goat IgG

AF771

100 µg

$355

None

Polyclonal

Goat IgG

AF-263-NA

100 µg

$355

None

32437

Mouse IgG2A

MAB263

500 µg

$325

None

Polyclonal

Goat IgG

AF563

100 µg

$355

Polyclonal

Goat IgG

AF1007

100 µg

$355

IL-1 RII in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-1 receptor II (IL-1 RII) was detected in human PBMCs by indirect immunofluorescent staining using anti-human IL-1 RII affinitypurified polyclonal antibody (Catalog # AF-263-NA) and counterstained (green).

IL-1 RAPL2/IL-1 R9

62

Human

None



SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Bovine

None

Polyclonal

Goat IgG

AF2465

100 µg

$355

Canine

Biotin

Polyclonal

Goat IgG

BAF1815

50 µg

$380

None

Polyclonal

Goat IgG

AF1613

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1613

50 µg

$380

None

Polyclonal

Goat IgG

AF1890

100 µg

$355

Equine

Feline IL-2 Human

Porcine Rat

Biotin

Polyclonal

Goat IgG

BAF1890

None

Polyclonal

Goat IgG

AF-202-NA

Biotin

Polyclonal

Goat IgG

None

5334

Mouse IgG1

SIZE

PRICE

50 µg

$380

100 µg

$355

BAF202

50 µg

$380

MAB202

500 µg

$325

None

5355

Mouse IgG2A

MAB602

500 µg

$325

None

Polyclonal

Goat IgG

AF652

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF652

50 µg

$380

None

Polyclonal

Goat IgG

AF-502-NA

100 µg

$355

None

22722

Mouse IgG1

MAB223

500 µg

$325

None

24204

Mouse IgG1

MAB623

500 µg

$325

IL-2 in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-2 (IL-2) was detected in PMA- and ionomycin-stimulated human PBMCs using biotinylated anti-human IL-2 affinity-purified polyclonal antibody (Catalog # BAF202; red) and counterstained green.

Human IL-2 Ra

Mouse

None

Polyclonal

Goat IgG

AF2438

100 µg

$355

Rat

None

Polyclonal

Sheep IgG

AF5156

100 µg

$355

IL-2 Ra in Mouse Spleen. Interleukin-2 Receptor a (IL-2 Ra) was detected in cryostat tissue sections of mouse spleen using anti-mouse IL-2 Ra affinity-purified polyclonal antibody (Catalog # AF2438). Tissues were stained using NorthernLights-557 donkey antigoat IgG secondary antibody (Catalog # NL001; red). Tissues were counterstained green.

IL-2 Rb

Human

Human IL-3 Rat IL-3 Ra

Human

None

27302

Mouse IgG1

MAB224

500 µg

$325

None

Polyclonal

Total Goat IgG

AB-203-NA

1 mg

$355

None

Polyclonal

Goat IgG

AF-203-NA

100 µg

$355

None

4806

Mouse IgG1

MAB203

500 µg

$325

None

Polyclonal

Goat IgG

AF2524

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2524

50 µg

$380

None

32703

Mouse IgG1

MAB301

500 µg

$325


63


IL-4

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Bovine

None

Polyclonal

Goat IgG

AF2469

100 µg

$355

Equine

None

Polyclonal

Goat IgG

AF1809

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF204

50 µg

$380

None

3007

Mouse IgG1

MAB304

500 µg

$325

Porcine

None

Polyclonal

Goat IgG

AF654

100 µg

$355

Rat

Biotin

Polyclonal

Goat IgG

BAF504

50 µg

$380

Human

SIZE

PRICE

IL-4 in Human Lymph Node. Interleukin-4 (IL-4) was detected in a cryostat tissue section of frozen human lymph node using anti-human IL-4 monoclonal antibody (Catalog # MAB304). Tissues were stained using anti-mouse secondary antibody (brown) and counterstained with hematoxylin (blue).

IL-4 Ra

IL-5

Human

None

25463

Mouse IgG2A

MAB230

500 µg

$325

Canine

None

Polyclonal

Goat IgG

AF1964

100 µg

$355

Equine

None

Polyclonal

Goat IgG

AF2470

100 µg

$355

Feline

None

Polyclonal

Goat IgG

AF1795

100 µg

$355

Human

None

9906

Mouse IgG1

MAB605

500 µg

$325

Mouse

None

Polyclonal

Goat IgG

AF-405-NA

100 µg

$355

Porcine

None

Polyclonal

Goat IgG

AF3137

100 µg

$355

None

Polyclonal

Goat IgG

AF545

100 µg

$355

None

299298

Mouse IgG1

MAB5452

100 µg

$240

None

Polyclonal

Goat IgG

AF1609

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1609

50 µg

$380

None

247002

Mouse IgG2B

MAB16091

500 µg

$325

Rat

Canine

Equine Feline IL-6 Human

None

Polyclonal

Goat IgG

AF1886

100 µg

$355

None

Polyclonal

Goat IgG

AF2305

100 µg

$355

None

341031

Mouse IgG2B

MAB23051

100 µg

$240

None

Polyclonal

Goat IgG

AF-206-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF206

50 µg

$380

None

1936

Mouse IgG2B

MAB2061

500 µg

$325 $355

Mouse

None

Polyclonal

Goat IgG

AF-406-NA

100 µg

Porcine

None

77830

Mouse IgG1

MAB686

500 µg

$325

Rat

None

Polyclonal

Goat IgG

AF506

100 µg

$355

None

Polyclonal

Goat IgG

AF1830

100 µg

$355

Biotin

255821

Rat IgG2A

BAM1830

100 µg

$380

None

255821

Rat IgG2A

MAB1830

500 µg

$325

None

255820

Rat IgG1

MAB18301

100 µg

$240

IL-6 in Porcine Peripheral Blood Mononuclear Cells. Interleukin-6 (IL-6) was detected in porcine PBMCs using anti-porcine IL-6 monoclonal antibody (Catalog # MAB686). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

IL-6 Ra

64

Mouse



LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

IL-7

MOLECULE

Human

None

7417

Mouse IgG1

MAB207

500 µg

$325

CXCR1/IL-8 RA

Human

None

42705

Mouse IgG2A

MAB330

500 µg

$325

CXCR2/IL-8 RB

Human

None

48311

Mouse IgG2A

MAB331

500 µg

$325

Equine

None

Polyclonal

Goat IgG

AF1605

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF-217-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF693

100 µg

$355

None

262715

Mouse IgG2B

MAB6932

100 µg

$240

Rat

None

Polyclonal

Goat IgG

AF519

100 µg

$355

IL-10 Ra

Human

None

Polyclonal

Goat IgG

AF-274-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF-219-NA

100 µg

$355

IL-12

Human

Biotin

Polyclonal

Goat IgG

BAF219

100 µg

$380

None

24910

Mouse IgG1

MAB219

500 µg

$325

IL-10

Porcine

IL-12 p70

Human

None

24945

Mouse IgG1

MAB611

500 µg

$325

IL-12/IL-23 p40

Feline

None

Polyclonal

Goat IgG

AF1954

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF-413-NA

100 µg

$355

Rat

Biotin

296202

Mouse IgG2B

BAM1945

100 µg

$380

Human

None

Polyclonal

Goat IgG

AF152

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF146

100 µg

$355

IL-13 IL-13 Ra1 IL-13 Ra2

IL-15

IL-15 Ra

Mouse

None

Polyclonal

Goat IgG

AF539

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF315

100 µg

$355

None

Polyclonal

Goat IgG

AF447

100 µg

$355

Mouse Human

Biotin

Polyclonal

Goat IgG

BAF447

50 µg

$380

None

Polyclonal

Goat IgG

AF247

100 µg

$355

IL-16 C-terminal Peptide

Human

None

Polyclonal

Goat IgG

AF-316-PB

100 µg

$355

IL-17

Human

None

Polyclonal

Goat IgG

AF-317-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF1207

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1207

50 µg

$380

None

Polyclonal

Goat IgG

AF1040

100 µg

$355

IL-17B R

Human Mouse

IL-17C IL-17D

Human Human

None

Polyclonal

Goat IgG

AF1234

100 µg

$355

None

Polyclonal

Goat IgG

AF1504

100 µg

$355

None

246018

Mouse IgG2B

MAB15041

100 µg

$240

IL-17D in Human Heart. Interleukin-17D (IL-17D) was detected in a paraffin-embedded human heart section using anti-human IL-17D monoclonal antibody (Catalog # MAB15041). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

IL-17F

Human

None

Polyclonal

Goat IgG

AF1335

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF2057

100 µg

$355


65


IL-17 RD/SEF

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF2275

100 µg

$355

None

Polyclonal

Goat IgG

AF2276

100 µg

$355

None

400210

Rat IgG2A

MAB2276

100 µg

$240

Mouse

IL-17 RD in Mouse Liver. Interleukin-17 Receptor D (IL-17 RD) was detected in frozen mouse liver sections using anti-mouse IL-17 RD monoclonal antibody (Catalog # MAB2276; red) and counterstained green.

Canine IL-18/IL-1F4

IL-18 Ra/IL-1 R5

SIZE

PRICE

IL-17 RD in Human Kidney. Interleukin-17 Receptor D (IL-17 RD) was detected in a paraffin-embedded human kidney sections using anti-human IL-17 RD affinity-purified polyclonal antibody (Catalog # AF2275). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

None

Polyclonal

Goat IgG

AF2924

100 µg

$355

None

314811

Mouse IgG1

MAB29241

100 µg

$240

Porcine

None

Polyclonal

Goat IgG

AF588

100 µg

$355

Rhesus Macaque

None

Polyclonal

Goat IgG

AF2548

100 µg

$355

None

Polyclonal

Goat IgG

AF840

100 µg

$355

None

70625

Mouse IgG1

MAB840

500 µg

$325

Human

IL-18 R in Human Skin. Interleukin-18 Receptor (IL18 R) was detected in frozen sections of human skin using anti-human IL-18 R affinity-purified polyclonal antibody (Catalog # AF840). Tissue was stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue).

IL-20 Ra

Human

None

173707

Mouse IgG1

MAB11761

500 µg

$325

IL-21

Mouse

None

Polyclonal

Goat IgG

AF594

100 µg

$355

IL-21 in Mouse Splenocytes. Interleukin-21 (IL-21) was detected in mouse splenocytes stimulated with concanavalin A using anti-mouse IL-21 affinity-purified polyclonal antibody (Catalog # AF594). Cells were stained using NorthernLights-557 anti-goat secondary antibody (Catalog # NL001; red) and counterstained green.

IL-21 R Subunit

Mouse

None

Polyclonal

Goat IgG

AF596

100 µg

$355

IL-22

Human

None

Polyclonal

Goat IgG

AF782

100 µg

$355

Human

None

305405

Mouse IgG1

MAB2770

100 µg

$240 $240

IL-22 Ra1

IL-24

Mouse

None

496514

Rat IgG2A

MAB42941

100 µg

Human

None

283123

Mouse IgG2B

MAB19651

100 µg

$240

None

Polyclonal

Goat IgG

AF2786

100 µg

$355

Mouse

Biotin

303308

Rat IgG2A

BAM2786

100 µg

$380

IL-27 p28

Mouse

None

Polyclonal

Goat IgG

AF1834

100 µg

$355

IL-28A/IFN-λ2

Human

None

Polyclonal

Goat IgG

AF1587

100 µg

$355

IL-31

Human

None

Polyclonal

Goat IgG

AF2824

100 µg

$355

None

308202

Mouse IgG1

MAB28241

100 µg

$240

IL-31 RA

Human

None

Polyclonal

Goat IgG

AF2769

100 µg

$355

66



IL-33

IL-33 Propeptide

SPECIES Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF3625

100 µg

$355

None

390412

Rat IgG2B

MAB3625

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF3626

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF4810

100 µg

$355

Mouse

None

Polyclonal

Sheep IgG

AF5010

100 µg

$355

IL-33 Propeptide in Human Umbilical Vein Endothelial Cells. Interleukin-33 (IL-33) Propeptide was detected in HUVECs using anti-human IL-33 Propeptide affinity-purified polyclonal antibody (Catalog # AF4810). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Indian Hedgehog N-Terminus

Mouse

None

Polyclonal

Goat IgG

AF1705

100 µg

$355

ING1

Human

None

Polyclonal

Goat IgG

AF5758

100 µg

$355

Insulin

Human/Mouse/Bovine

None

182410

Rat IgG2A

MAB1417

100 µg

$240

Proinsulin

Human/Mouse

Biotin

253627

Mouse IgG2A

BAM13361

100 µg

$240

None

253627

Mouse IgG2A

MAB13361

100 µg

$240

None

Polyclonal

Goat IgG

AF1544

100 µg

$355

None

243524

Mouse IgG2B

MAB1544

100 µg

$240

None

Polyclonal

Goat IgG

AF3055

100 µg

$355

Insulin R/CD220

Human

INSRR

Human

Integrin a1/CD49a

Human

Human Integrin a2/CD49b Mouse Integrin a2b/CD41 Integrin a3/CD49c

Integrin a4/CD49d

Mouse Human

Human

None

Polyclonal

Sheep IgG

AF5676

100 µg

$355

Biotin

HAS3

Mouse IgG2A

BAM1233

100 µg

$295

None

HAS3

Mouse IgG2A

MAB1233

500 µg

$325

None

430903

Rat IgG2A

MAB12332

100 µg

$240

None

Polyclonal

Sheep IgG

AF1740

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF1740

50 µg

$380

None

386629

Rat IgG2A

MAB41181

100 µg

$240

Biotin

IA3

Mouse IgG1

BAM1345

100 µg

$295

None

IA3

Mouse IgG1

MAB1345

500 µg

$325

Biotin

7.2R

Mouse IgG1

BAM1354

100 µg

$295

None

7.2R

Mouse IgG1

MAB1354

500 µg

$325

Integrin a4/CD49d in Human U937 Cells. Integrin a4/CD49d was detected in human U937 lymphoma cell line using anti-human integrin a4/CD49d monoclonal antibody (Catalog # MAB1354). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

Integrin a5/CD49e

Human

None

238307

Mouse IgG1

MAB1864

100 µg

$240

Mouse

None

235112

Rat IgG2B

MAB1754

100 µg

$240

Integrin a5 in Human Fibrosarcoma Cell Line (HT1080). Integrin a5 was detected in HT1080 human fibrosarcoma cells using anti-human integrin a5 monoclonal antibody (Catalog # MAB1219). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).


67

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Integrin a6/CD49f

Integrin a8

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

MP4F10

Mouse IgG2B

MAB1350

500 µg

$325

Human/Mouse/Bovine

None

GoH3

Rat IgG2A

MAB13501

100 µg

$240

None

Polyclonal

Goat IgG

AF4076

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF4076

50 µg

$380

Biotin

ICRF44

Mouse IgG1

BAM1699

100 µg

$240

None

ICRF44

Mouse IgG1

MAB1699

500 µg

$325

None

238446

Mouse IgG2B

MAB16991

100 µg

$240

Biotin

M1/70

Rat IgG2B

BAM1124

250 µg

$380

None

M1/70

Rat IgG2B

MAB1124

500 µg

$325

None

Polyclonal

Goat IgG

AF1219

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1219

50 µg

$380

None

P2W7

Mouse IgG1

MAB1219

500 µg

$325

Mouse

Human Integrin aM/CD11b Mouse

SIZE

PRICE

Integrin aV/CD51

Human

None

273210

Mouse IgG1

MAB12191

100 µg

$240

Integrin aVb3

Human

None

23C6

Mouse IgG1

MAB3050

100 µg

$240

Integrin aVb5

Human

None

P5H9

Mouse IgG1

MAB2528

100 µg

$240

Integrin b1/CD29

Human

None

4B7R

Mouse IgG1

MAB1778

100 µg

$240

None

P5D2

Mouse IgG1

MAB17781

100 µg

$240

None

P4G11

Mouse IgG1

MAB17782

100 µg

$240

Human Integrin b1/CD29 Mouse

None

419127

Mouse IgG2B

MAB17783

100 µg

$240

None

Polyclonal

Goat IgG

AF2405

100 µg

$355

None

265917

Rat IgG2A

MAB2405

100 µg

$240

Integrin b1, Orexin b, and p38a in Mouse Brainstem. Cryostat sections of mouse brainstem were labeled with anti-human Orexin B monoclonal antibody (Catalog # MAB734), anti-human/mouse/rat p38a affinitypurified polyclonal antibody (Catalog # AF8691), and anti-mouse Integrin b1 affinity-purified polyclonal antibody (Catalog #AF2405). Tissue was stained with NorthernLights (NL)-conjugated secondary polyclonal antibodies: NL-493 donkey anti-rabbit (Catalog # NL006; green), NL-557 donkey anti-mouse (Catalog # NL007; red), and NL-637 donkey anti-goat (Catalog # NL002; blue). Immunohistochemical image of integrin b1 expression is pseudo-colored for presentation.

Integrin b2/CD18

Integrin b4/CD104

Human Human Mouse

Integrin b5

Human

Human Integrin b6 Mouse

Integrin b7

68

Human

None

Polyclonal

Goat IgG

AF1730

100 µg

$355

None

Polyclonal

Sheep IgG

AF4060

100 µg

$355

None

422325

Mouse IgG2B

MAB4060

100 µg

$240

None

308601

Rat IgG2A

MAB4054

100 µg

$240

None

Polyclonal

Sheep IgG

AF3824

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF3824

50 µg

$380

None

Polyclonal

Sheep IgG

AF4155

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF4155

50 µg

$380

None

437211

Mouse IgG2B

MAB4155

100 µg

$240

None

Polyclonal

Goat IgG

AF2389

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2389

50 µg

$380

None

268622

Rat IgG2A

MAB2389

100 µg

$240

None

473207

Mouse IgG1

MAB4669

100 µg

$240


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Integrin b8

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

416911

Mouse IgG1

MAB47751

100 µg

$240

Polyclonal

Sheep IgG

AF4254

100 µg

$355

Integrin b8 in Human A172 Cells. Integrin b8 was detected in regions of membrane ruffling in A172 human glioblastoma cells using anti-human Integrin b8 monoclonal antibody (Catalog # MAB4775). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

Intelectin-1/Omentin

Human

None

Intelectin-1 in Mouse Intestine. Intelectin-1 was detected in a section of mouse intestine using antimouse Intelectin-1 affinity-purified polyclonal antibody (Catalog # AF4254; red). The tissue was stained using NorthernLights-557 anti-sheep secondary antibody (Catalog # NL010) and the nuclei counterstained with DAPI (blue).

IRAK4

Human

None

Polyclonal

Goat IgG

AF3919

100 µg

$355

IRF3

Human

None

Polyclonal

Goat IgG

AF4019

100 µg

$355

IRF5 aa 214-395

Human

None

Polyclonal

Sheep IgG

AF4508

100 µg

$355

IRF8

Human

None

Polyclonal

Sheep IgG

AF5117

100 µg

$355

IRS-1

Human

None

395601

Mouse IgG1

MAB39781

100 µg

$240

ISG15/UCRP aa 2-157

Human

None

539442

Mouse IgG2B

MAB4845

100 µg

$240

Islet-1

Human

None

Polyclonal

Goat IgG

AF1837

100 µg

$355

Islet-1 NorthernLights-conjugated

Human

NL557

Polyclonal

Goat IgG

NL1837R

500 µL

$350

Islet-2

Human

None

Polyclonal

Sheep IgG

AF4244

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF4244

50 µg

$380

Islet-2 and Plexin A3 in Embryonic Mouse Spinal Cord. Islet-2 and Plexin A3 were detected in a section of embryonic mouse spinal cord using biotinylated anti-human Islet-2 affinity-purified polyclonal antibody (Catalog # BAF4244) and anti-mouse Plexin A3 (Catalog # AF4075). The tissue was stained using NorthernLights-493 streptavidin (Catalog # NL997; green) and NorthernLights-557 donkey anti-goat IgG affinity-purified polyclonal antibody (Catalog # NL001; red), respectively. The nuclei were stained using DAPI (blue).

ISLR-2

Mouse

None

Polyclonal

Sheep IgG

AF4650

100 µg

$355

Jagged 1

Human

None

Polyclonal

Goat IgG

AF1277

100 µg

$355

Jagged 2

Human

None

Polyclonal

Goat IgG

AF1726

100 µg

$355

Jak1 aa 14-55

Mouse/Rat

None

Polyclonal

Goat IgG

AF602

100 µg

$355

Jak1 aa 20-274

Human/Mouse/Rat

None

413104

Rat IgG2B

MAB4260

100 µg

$240

Jak1 in Human Skin. Jak1 was detected in a section of paraffin-embedded human epidermis using antihuman Jak1 monoclonal antibody (Catalog # MAB4260). Tissue was stained using anti-rat HRP-DAB reagents (brown) and counterstained with hematoxylin (blue).


69

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE JAM-A

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF1103

100 µg

SIZE

PRICE $355

Mouse

None

Polyclonal

Goat IgG

AF1077

100 µg

$355

JAM-C

Mouse

None

Polyclonal

Goat IgG

AF1213

100 µg

$355

JNK Pan Specific

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF1387

100 µg

$285

Phospho-JNK (T183/Y185)

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF1205

50 µg

$285

JNK1/JNK2

Human/Mouse/Rat

None

252323

Mouse IgG2A

MAB2076

100 µg

$240

JNK1

Human/Mouse/Rat

None

228601

Mouse IgG1

MAB17761

100 µg

$240

JNK2

Human/Mouse/Rat

None

252320

Mouse IgG2B

MAB1846

100 µg

$240

c-Jun aa 1-331

Human/Mouse

None

320831

Mouse IgG1

MAB2670

100 µg

$240

Kallikrein 3/PSA

Human

None

Polyclonal

Goat IgG

AF1344

100 µg

$355

Kallikrein 5

Human

None

Polyclonal

Goat IgG

AF1108

100 µg

$355

Kallikrein 6/Neurosin

Human

None

Polyclonal

Goat IgG

AF2008

100 µg

$355

Polyclonal

Goat IgG

AF2624

100 µg

$355

Polyclonal

Goat IgG

AF3486

100 µg

$355

None

Polyclonal

Goat IgG

AF1595

100 µg

$355

None

189308

Mouse IgG3

MAB1595

500 µg

$325

Kallikrein 6 in Human Breast Cancer. Kallikrein 6/ Neurosin was detected in paraffin-embedded human breast cancer tissue sections using anti-human Kallikrein 6 affinity-purified polyclonal antibody (Catalog # AF2008). Tissues were stained using anti-goat HRPDAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Kallikrein 7

Human

None

Kallikrein 7 in Normal Human Skin. Kallikrein 7 (KLK7) was detected in paraffin-embedded normal human skin sections using anti-human KLK7 affinitypurified polyclonal antibody (Catalog # AF2624). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Kallikrein 9

Human

None

Kallikrein 9 in Human Ovarian Cancer. Kallikrein 9 (KLK9) was detected in paraffin-embedded human ovarian cancer sections using anti-human KLK9 affinity-purified polyclonal antibody (Catalog # AF3486). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Kallikrein 11

Human

Kallikrein 12

Human

None

Polyclonal

Sheep IgG

AF3095

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF2625

100 µg

$355

Kallikrein 13

Kallikrein 13 in Human Prostate Cancer. Kallikrein 13 (KLK13) was detected in paraffin-embedded human prostate cancer sections using anti-human KLK13 affinity-purified polyclonal antibody (Catalog # AF2625). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

70


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Kallikrein 15 KLF4

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF2540

100 µg

SIZE

PRICE $355

Human

None

Polyclonal

Goat IgG

AF3640

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF3158

100 µg

$355

KLF5

Human

None

Polyclonal

Goat IgG

AF3758

100 µg

$355

Klotho

Mouse

None

Polyclonal

Goat IgG

AF1819

100 µg

$355

None

236214

Rat IgG2A

MAB1819

500 µg

$325

None

387301

Mouse IgG1

MAB3895

100 µg

$240

None

387310

Mouse IgG1

MAB38951

100 µg

$240

Klotho in Mouse Kidney. Klotho was detected in a frozen section of mouse kidney tissue using antimouse Klotho affinity-purified polyclonal antibody (Catalog # AF1819). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

KOR

Kremen-1 Kremen-1 Laminin a1

Human Human

None

Polyclonal

Goat IgG

AF2127

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF1647

100 µg

$355

Mouse

Biotin

Polyclonal

Goat IgG

BAF1647

50 µg

$380

Mouse

None

AL-4

Rat IgG2A

MAB4656

100 µg

$240

Laminin a1 in Mouse Decidua. Laminin a1 was detected in frozen sections of mouse E10.5 decidua using anti-mouse laminin a1 monoclonal antibody (Catalog # MAB4656; red). Tissue was counterstained with DAPI (blue).

Laminin a1 N-Terminus Domain VI

Human

None

Polyclonal

Goat IgG

AF4187

100 µg

$355

None

Polyclonal

Goat IgG

AF3837

100 µg

$355

Laminin a4

Mouse

Biotin

Polyclonal

Goat IgG

BAF3837

50 µg

$380

Laminin g1

Human/Rat

None

D18

Mouse IgG2A

MAB2139

100 µg

$240

Laminin S

Human/Rat/Chicken

None

C4

Mouse IgG1

MAB2066

100 µg

$295

Laminin-1

Mouse

None

AL-2

Rat IgG1

MAB2549

100 µg

$240

None

AL-3

Rat IgG1

MAB25491

100 µg

$240

Laminin-5

Human

None

P3H9-2

Mouse IgG1

MAB2144

250 µg

$240

Laminin-5 in Human MCF10A Cells. Laminin-5 was detected in human MCF10A breast epithelial cell line using anti-human Laminin-5 monoclonal antibody (Catalog # MAB2144). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

LAMP1/CD107a Lumenal Domain

Human

None

Polyclonal

Sheep IgG

AF4800

100 µg

$355

Langerin/CD207

Human

None

Polyclonal

Goat IgG

AF2088

100 µg

$355

Lck

Human

None

355713

Mouse IgG2B

MAB3704

100 µg

$240

Human LDL R

Mouse

None

Polyclonal

Goat IgG

AF2148

100 µg

$355

None

Polyclonal

Goat IgG

AF2255

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2255

50 µg

$380


71

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE LEDGF Leptin/OB

Leptin R

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF3468

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF398

100 µg

$355

Mouse Human Mouse

SIZE

PRICE

None

Polyclonal

Goat IgG

AF498

100 µg

$355

None

Polyclonal

Goat IgG

AF389

100 µg

$355

None

52263

Mouse IgG2B

MAB867

500 µg

$325

None

Polyclonal

Goat IgG

AF497

100 µg

$355

None

Polyclonal

Goat IgG

AF-250-NA

100 µg

$355

None

9824

Mouse IgG2B

MAB250

500 µg

$325

Leptin Receptor in Rat Brain. Leptin Receptor was detected in cryostat tissue sections of rat brain using anti-mouse Leptin Receptor affinity-purified polyclonal antibody (Catalog # AF497). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

LIF

Human Mouse

None

Polyclonal

Total Goat IgG

AB-449-NA

1 mg

$355

LILRC1

Rat

None

Polyclonal

Goat IgG

AF4074

100 µg

$355

LIM1

Human

None

320416

Mouse IgG2A

MAB2725

100 µg

$240

Limitin

Mouse

None

Polyclonal

Goat IgG

AF597

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF597

50 µg

$380

LIN-28

Human

None

Polyclonal

Goat IgG

AF3757

100 µg

$355

LIN-41

Human

None

Polyclonal

Sheep IgG

AF5104

100 µg

$355 $355

LINGO-1

Human

None

Polyclonal

Goat IgG

AF3086

100 µg

LINGO-1 aa 40-556

Human

None

332237

Mouse IgG1

MAB30861

100 µg

$240

None

Polyclonal

Goat IgG

AF3679

100 µg

$355

None

382007

Mouse IgG2A

MAB36791

100 µg

$240

None

Polyclonal

Goat IgG

AF3885

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF3885

50 µg

$380

LINGO-2

Human

Lipin 1

Human

Lipin 3

Mouse

Lipocalin-2/NGAL

Human

LMIR3/CD300LF

Human

LMO2 LRRC4

Human Human

None

Polyclonal

Goat IgG

AF4064

100 µg

$355

None

Polyclonal

Goat IgG

AF1757

100 µg

$355

None

220310

Rat IgG2A

MAB1757

500 µg

$325

None

234903

Rat IgG2B

MAB2774

100 µg

$240

None

Polyclonal

Goat IgG

AF2726

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2726

50 µg

$380

None

Polyclonal

Sheep IgG

AF4995

100 µg

$355

LRRC4 in Human Cerebellum. Leucine rich repeat/ LRR-containing protein 4 (LRRC4) was detected in paraffin-embedded sections of human cerebellum using anti-human LRRC4 affinity-purified polyclonal antibody (Catalog # AF4995). Tissue was stained using anti-sheep HRP-DAB secondary reagents (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

72


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE LRRN1/NLRR-1

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Sheep IgG

AF4990

100 µg

$355

Polyclonal

Sheep IgG

AF5589

100 µg

$355

LRRN1 in Mouse Somites. Leucine rich repeat neuronal 1 (LRRN1) was detected in a frozen section from E9.5 mouse somite using anti-human LRRN1 affinitypurified polyclonal antibody (Catalog # AF4990). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

LRRTM2 Lumican

LXRa/NR1H3 Ligand Binding Domain

Lymphotoxin bR/TNFRSF3

Lyn aa 1-67

Lyp

Human/Mouse Human

None

Polyclonal

Goat IgG

AF2846

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF2745

100 µg

$355

None

PPZ0412

Mouse IgG2A

PP-PPZ0412-00

100 µg

$380

Human

Mouse

None

PPZ0412

Mouse IgG2A

PP-PPZ0412-10

100 µg

$380

None

Polyclonal

Goat IgG

AF1008

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1008

50 µg

$380 $355

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF3206

100 µg

Human/Rat

None

338716

Mouse IgG2B

MAB3206

100 µg

$240

None

Polyclonal

Goat IgG

AF3428

100 µg

$355

None

340113

Mouse IgG2B

MAB3428

100 µg

$240

None

Polyclonal

Goat IgG

AF2089

100 µg

$355

Human

Human LYVE-1 Mouse

MAdCAM-1

Mouse

MafF

Human

MAG/Siglec-4a

None

Rat

Biotin

Polyclonal

Goat IgG

BAF2089

50 µg

$380

None

Polyclonal

Goat IgG

AF2125

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2125

50 µg

$380

None

223322

Rat IgG2A

MAB2125

500 µg

$325

None

Polyclonal

Goat IgG

AF993

100 µg

$355

None

Polyclonal

Goat IgG

AF3917

100 µg

$355

None

Polyclonal

Goat IgG

AF538

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF538

50 µg

$380

None

Polyclonal

Goat IgG

AF2567

100 µg

$355

None

290622

Rat IgG2A

MAB2567

100 µg

$240

None

Polyclonal

Goat IgG

AF3044

100 µg

$355

None

Polyclonal

Goat IgG

AF932

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF932

50 µg

$380

None

542808

Mouse IgG2B

MAB828

100 µg

$240

MAG/Siglec-4a in Rat Cerebellum. Myelin-associated Glycoprotein (MAG/Siglec-4a) was detected in cryostat sections of rat cerebellum using anti-rat MAG affinity-purified polyclonal antibody (Catalog # AF538). Tissues were stained using the anti-goat HRP DAB Cell and Tissue Staining Kit (Catalog # CTS008); brown) and counterstained with hematoxylin (blue).

Mash1

Mouse

Matrilin-2

Human

MCAM/CD146

Human

Mcl-1 aa 1-230

Human

M-CSF R

Mouse

None

Polyclonal

Sheep IgG

AF3818

100 µg

$355

Phospho-M-CSF R (Y723)

Human

None

Polyclonal

Rabbit IgG

AF3268

50 µg

$285

MD-1

Human

None

Polyclonal

Goat IgG

AF925

100 µg

$355

MD-2

Human

Biotin

Polyclonal

Sheep IgG

BAF1787

50 µg

$380

MDM2

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF1244

100 µg

$285

MEK1/MEK2

Human/Mouse/Rat

None

239416

Mouse IgG2B

MAB2678

100 µg

$240

Phospho-MEK1/MEK2 (MEK1 S218/S2220, MEK2 S222/S226)

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF2506

100 µg

$285


73


LABEL

CLONE

HOST/TYPE

CATALOG #

MEK2

MOLECULE

Human/Mouse/Rat

None

300317

Mouse IgG2A

MAB2855

100 µg

$240

MELK

Human

None

Polyclonal

Sheep IgG

AF4820

100 µg

$355

Melusin

Human

None

Polyclonal

Goat IgG

AF2740

100 µg

$355

None

308705

Mouse IgG2B

MAB2740

100 µg

$240

Meprin a Subunit/MEP1A

Human

None

Polyclonal

Goat IgG

AF3220

100 µg

$355

Meprin b Subunit/MEP1B

SIZE

PRICE

Human

None

Polyclonal

Goat IgG

AF2895

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF3300

100 µg

$355

Meprin b Subunit in Mouse Intestine. Meprin b Subunit (MEP1B) was detected in a frozen section of mouse intestine using anti-mouse MEP1B affinitypurified polyclonal antibody (Catalog # AF3300). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

MESDC2

Human

None

Polyclonal

Sheep IgG

AF5577

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF4545

100 µg

$355

MESDC2 in Adult Mouse Testis. Mesoderm development candidate 2 (MESDC2) was detected in section of mouse testis using anti-mouse MESDC2 affinity-purified polyclonal antibody Catalog # AF4545). The tissue was stained using anti-goat NorthernLight-557 secondary antibody (Catalog # NL001, red) and the nuclei were counterstained with DAPI (blue).

Mesothelin Isoform 1, aa 296-580 Meteorin

Human Mouse

None

420404

Rat IgG2A

MAB32651

100 µg

$240

None

Polyclonal

Goat IgG

AF3475

100 µg

$355

None

347505

Rat IgG2B

MAB3475

100 µg

$240

Meteorin in Rat Cortical Stem Cells. Meteorin was detected in 7-day differentiated rat cortical stem cells using anti-mouse Meteorin monoclonal antibody (Catalog # MAB3475). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).

MFRP

Human

None

291204

Rat IgG1

MAB19151

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF3445

100 µg

$355

mGluR1a C-Terminus

Rat

None

Polyclonal

Rabbit IgG

PPS077

100 µL

$355

mGluR3

Human

None

Polyclonal

Sheep IgG

AF4668

100 µg

$355

mGluR5

Human

None

Polyclonal

Sheep IgG

AF4514

100 µg

$355

None

464823

Mouse IgG2B

MAB45141

100 µg

$240

mGluR5 in Human Brain (Putamen). Metabotropic glutamate receptor 5 (mGluR5) was detected in a paraffin-embedded human brain (putamen) section using anti-human mGluR5 affinity-purified polyclonal antibody (Catalog # AF4514). The tissue was stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).

74



LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

mGluR5/1a

MOLECULE

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS079

100 µL

$355

mGluR8

Human

None

476410

Mouse IgG2B

MAB5277

100 µg

$240

MIA

Human

None

Polyclonal

Goat IgG

AF2050

100 µg

$355

None

294203

Mouse IgG2B

MAB20501

100 µg

$240

None

Polyclonal

Goat IgG

AF1300

100 µg

$355

MICA

MICL/CLEC12A

Human Human

Biotin

Polyclonal

Goat IgG

BAF1300

50 µg

$380

None

Polyclonal

Goat IgG

AF2946

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF2950

100 µg

$355

Midkine

Human

None

Polyclonal

Goat IgG

AF-258-PB

100 µg

$355

Mineralocorticoid R/NR3C2

Human

None

H3122

Mouse IgG2A

PP-H3122-00

100 µg

$380

Mineralocorticoid R/NR3C2 aa 1-670

Human

None

385707

Mouse IgG2B

MAB4369

100 µg

$240

MIS/AMH

Rat

None

Polyclonal

Goat IgG

AF1446

100 µg

$355

MIS/AMH Propeptide MIS RII

Human

None

Polyclonal

Goat IgG

AF2748

100 µg

$355

Human

None

Polyclonal

Sheep IgG

AF4749

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF1618

100 µg

$355

MITF

Human

None

Polyclonal

Goat IgG

AF5769

100 µg

$355

MIXL1

Human

None

319919

Mouse IgG2A

MAB2610

100 µg

$240

MKK3

Human/Mouse/Rat

None

275909

Mouse IgG2B

MAB2515

100 µg

$240

MKK4

Human

None

323042

Mouse IgG2B

MAB3390

100 µg

$240

Phospho-MKK4 (S257/T261)

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF2990

100 µg

$285

MKK6

Human/Mouse/Rat

None

304328

Rat IgG2B

MAB1604

100 µg

$240

MKK7

Human

None

Polyclonal

Goat IgG

AF3579

100 µg

$355

None

324302

Mouse IgG2B

MAB3579

100 µg

$240

MLK4a aa 560-570

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF3435

100 µg

$285

Pan MLK-4 in Human Colon Cancer. Mixed lineage Kinase-4 (MLK-4) was detected in paraffin-embedded human colon cancer sections using anti-human/ mouse/rat pan MLK-4 affinity-purified polyclonal antibody (Catalog # AF3435). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using antirabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).

MLX

Human

MMP-1

Human

Pro MMP-1

Human

MMP-2

Human Mouse/Rat

MMP-3

Human

None

Polyclonal

Goat IgG

AF4186

100 µg

$355

None

Polyclonal

Goat IgG

AF901

100 µg

$355

None

36665

Mouse IgG1

MAB901

200 µg

$295

None

36660

Mouse IgG1

MAB900

200 µg

$295

None

Polyclonal

Goat IgG

AF902

100 µg

$355

None

36006

Mouse IgG1

MAB902

200 µg

$295

None

Polyclonal

Goat IgG

AF1488

100 µg

$355

None

Polyclonal

Goat IgG

AF513

100 µg

$355

None

50647

Mouse IgG1

MAB513

500 µg

$325

None

10D6

Mouse IgG1

MAB905

200 µg

$295

MMP-3 in Human Osteosarcoma Cell Line (MG63). Matrix Metalloproteinase-3 (MMP-3) was detected in MG-63 cells using anti-human MMP-3 monoclonal antibody (Catalog # MAB513). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).


75


MMP-7

Pro MMP-7

MMP-8

SPECIES Human

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Polyclonal

Goat IgG

AF907

100 µg

$355

None

111433

Mouse IgG2B

MAB9071

500 µg

$325 $355

Mouse

None

Polyclonal

Goat IgG

AF2967

100 µg

Human

None

6A4

Mouse IgG1

MAB907

200 µg

$295

None

Polyclonal

Goat IgG

AF908

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF908

None

100608

Mouse IgG2A

MAB9081

None

Polyclonal

Total Goat IgG

None

Polyclonal

Human

Human MMP-9

Mouse

MMP-10

LABEL None

Human

50 µg

$380

500 µg

$325

AB911

1 mg

$355

Goat IgG

AF911

100 µg

$355 $295

None

4H3

Mouse IgG1

MAB911

200 µg

None

36020

Mouse IgG1

MAB936

500 µg

$325

None

Polyclonal

Goat IgG

AF909

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF909

50 µg

$380

None

Polyclonal

Goat IgG

AF910

100 µg

$355

None

110304

Mouse IgG1

MAB910

500 µg

$325

None

117239

Mouse IgG2B

MAB9101

500 µg

$325

None

110316

Mouse IgG1

MAB9102

500 µg

$325

MAB919

500 µg

$325

MMP-12 Catalytic Domain

Human

None

82902

Mouse IgG2B

MMP-12 Hemopexin Domain

Human

None

4D2

Mouse IgG1

MAB917

200 µg

$295

None

Polyclonal

Goat IgG

AF511

100 µg

$355

None

87512

Mouse IgG1

MAB511

500 µg

$325

MMP-13

Human

MMP-13 in Human Ovarian Cancer Tissue. Matrix metalloproteinase-13 (MMP-13) producing cells in human ovarian cancer tissue were detected using anti-human MMP-13 affinity-purified antibody (Catalog # AF511). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and Hematoxylin (blue) counterstain.

Pro MMP-13

Human

None

87518

Mouse IgG2B

MAB913

500 µg

$325

None

Polyclonal

Goat IgG

AF918

100 µg

$355

None

128527

Mouse IgG2B

MAB9181

500 µg

$325

MMP-14

Human

MMP-14 aa 165-175

Human

None

5H2

Mouse IgG1

MAB918

500 µg

$325

MMP-15

Human

None

130522

Mouse IgG1

MAB9161

500 µg

$325

MMP-15 Catalytic Domain

Human

None

130516

Mouse IgG1

MAB916

500 µg

$325 $355

MMP-24/MT5-MMP

Human/Mouse

None

Polyclonal

Total Rabbit IgG

AB924

200 µg

MMP-25/MT6-MMP Hemopexin Domain

Human

None

141825

Mouse IgG1

MAB11421

500 µg

$325

None

Polyclonal

Goat IgG

AF2534

100 µg

$355

None

309210

Rat IgG2A

MAB2534

100 µg

$240 $355

MMR

MOG

Human Human

None

Polyclonal

Goat IgG

AF2395

100 µg

Human/Mouse

Biotin

296829

Rat IgG2B

BAM2439

100 µg

$380

None

Polyclonal

Goat IgG

AF2439

100 µg

$355

Mouse MRP1 MSK1

Biotin

Polyclonal

Goat IgG

BAF2439

50 µg

$380

Human

None

QCRL

Mouse IgG1

MAB19291

100 µg

$240

Human

None

252608

Mouse IgG2B

MAB2518

100 µg

$240

Human/Mouse

None

Polyclonal

Goat IgG

AF2518

100 µg

$355

Phospho-MSK1 (S212)

Human

None

Polyclonal

Rabbit IgG

AF1036

100 µg

$285

Phospho-MSK2 (S196)

Human/Mouse

None

Polyclonal

Rabbit IgG

AF189

100 µg

$285

MUCDHL

Human

None

Polyclonal

Goat IgG

AF3826

100 µg

$355

76


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Musashi-1

SPECIES Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF2628

100 µg

$355

None

282613

Mouse IgG2B

MAB2628

100 µg

$240

Musashi-1 in Human Intestine. Musashi-1 was detected in a paraffin-embedded human intestine section using anti-human Musashi-1 affinity-purified polyclonal antibody (Catalog # AF2628). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Mxi1

Human

None

Polyclonal

Goat IgG

AF4185

100 µg

$355

c-Myc aa 66-201

Human

None

Polyclonal

Goat IgG

AF3696

100 µg

$355

None

Polyclonal

Goat IgG

AF2928

100 µg

$355 $240

Human MyD88

Myeloperoxidase/MPO Myeloperoxidase/MPO aa 279-745

None

316603

Rat IgG2A

MAB2928

100 µg

Mouse

None

316902

Rat IgG2A

MAB3109

100 µg

$240

Mouse/Rat

None

Polyclonal

Goat IgG

AF3109

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF3174

100 µg

$355

Human/Mouse

None

Polyclonal

Goat IgG

AF3667

100 µg

$355

Human/Mouse

None

392105

Mouse IgG2B

MAB3174

100 µg

$240

Myeloperoxidase in Human Spleen. Myeloperoxidase (MPO) was detected in paraffin-embedded human spleen tissue sections using anti-human/mouse MPO monoclonal antibody (Catalog # MAB3174) and Antigen Retrieval Reagent - Basic (Catalog # CTS013). The tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Myosin Heavy Chain

Human

None

MF20

Mouse IgG2B

MAB4470

100 µg

$240

NAALADase-like 2/NAALADL2

Human

None

Polyclonal

Goat IgG

AF4665

100 µg

$355

None

Polyclonal

Goat IgG

AF1997

50 µg

$355

Nanog

Human Mouse

Biotin

Polyclonal

Goat IgG

BAF1997

50 µg

$395

None

Polyclonal

Goat IgG

AF2729

100 µg

$355

NL1997G

500 µL

$350

Nanog in Human BG01V Embryonic Stem Cells. Nanog was detected in human BG01V embryonic stem cells using anti-human Nanog affinity-purified polyclonal antibody (Catalog # AF1997). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Nanog NorthernLights-conjugated

Human

NL493

Polyclonal

Goat IgG

NCAM-1/CD56

Human

None

301021

Mouse IgG2B

MAB24081

100 µg

$240

NCAM-L1

Human

None

Polyclonal

Goat IgG

AF277

100 µg

$355

Nectin-3

Human

None

Polyclonal

Goat IgG

AF3064

100 µg

$355

Nectin-4

Human

None

Polyclonal

Goat IgG

AF2659

100 µg

$355

None

337506

Mouse IgG2A

MAB26591

100 µg

$240


77

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE NEDD8

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human/Mouse/Rat

None

Polyclonal

Sheep IgG

AF4936

100 µg

$355

Polyclonal

Goat IgG

AF1079

100 µg

$355

NEDD8 in Human Skeletal Muscle. Neural precursor cell-expressed developmentally down-regulated gene 8 (NEDD8) was detected in paraffin-embedded human skeletal muscle sections using anti-human/ mouse/rat NEDD8 affinity-purified polyclonal antibody (Catalog # AF4936). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Neogenin Nephrin

Mouse

None

Human

None

Polyclonal

Sheep IgG

AF4269

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF3159

100 µg

$355

Polyclonal

Goat IgG

AF1182

100 µg

$355 $380

Nephrin in Mouse Kidney. Nephrin was detected in frozen sections of mouse kidney using anti-mouse nephrin affinity-purified polyclonal antibody (Catalog # AF3159). Tissue was stained using anti-goat IgG NorthernLights-557-conjugated secondary antibody (Catalog # NL001; red) and counterstained green.

None

Human Neprilysin/CD10

Nestin

Biotin

Polyclonal

Goat IgG

BAF1182

50 µg

Human/Mouse

None

200103

Rat IgG2B

MAB1126

500 µg

$325

Mouse

None

Polyclonal

Goat IgG

AF1126

100 µg

$355

Human

None

196908

Mouse IgG1

MAB1259

100 µg

$240

None

Polyclonal

Goat IgG

AF2736

100 µg

$355

None

307501

Mouse IgG2A

MAB2736

100 µg

$240

$345

Rat

Nestin in Undifferentiated Rat Neural Stem Cells. Nestin was detected in rat cortical stem cells using anti-rat Nestin monoclonal antibody (Catalog # MAB2736). The cells were stained using anti-mouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and the nuclei counterstained with DAPI (blue).

Nestin NorthernLights-conjugated

Human

NL637

196908

Mouse IgG1

NL1259V

500 µL

Rat

NL557

307501

Mouse IgG2A

NL2736R

500 µL

$345

None

Polyclonal

Goat IgG

AF1254

100 µg

$355

Human Netrin-4 Mouse

Biotin

Polyclonal

Goat IgG

BAF1254

50 µg

$380

None

Polyclonal

Goat IgG

AF1132

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1132

50 µg

$380

349724

Rat IgG2A

MAB27441

100 µg

$240

Netrin-4 in Mouse Brain. Netrin-4 was detected in frozen sections of mouse brain (cerebellum) using anti-mouse Netrin-4 affinity-purified polyclonal antibody (Catalog # AF1132). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Netrin-G2a

78

Mouse

None



LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

NRG1-a/HRG1-a EGF Domain

MOLECULE

Human

None

Polyclonal

Goat IgG

AF-296-NA

100 µg

PRICE $355

NRG1-b1/HRG1-b1 EGF Domain

Human

None

Polyclonal

Goat IgG

AF-396-NA

100 µg

$355

Neuregulin-3/NRG3 EGF-like Domain

Mouse

None

Polyclonal

Goat IgG

AF753

100 µg

$355

Neurexin 3/NRXN3

Human/Mouse

None

Polyclonal

Sheep IgG

AF5269

100 µg

$355

Neuritin

Human

None

Polyclonal

Goat IgG

AF283

100 µg

$355

Neurocan

Mouse/Rat

NeuroD1

Human

NF-H

Human

None

Polyclonal

Sheep IgG

AF5800

100 µg

$355

None

Polyclonal

Goat IgG

AF2746

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2746

50 µg

$380

None

Polyclonal

Goat IgG

AF3108

100 µg

$355

Neurofilament-H in Human Brain. Neurofilament-H (NF-H) was detected in paraffin-embedded human brain (cerebellum) tissue sections using anti-human NF-H affinity-purified polyclonal antibody (Catalog # AF3108). Tissues were stained using anti-goat HRPDAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

NF-L

Human

None

268014

Mouse IgG2B

MAB2216

100 µg

$240

NF-M

Human

None

Polyclonal

Sheep IgG

AF3029

100 µg

$355

Neurogenin-2

Human/Rat

None

7G4

Mouse IgG2A

MAB3314

100 µg

$240

Human

None

Polyclonal

Sheep IgG

AF5685

100 µg

$355

Mouse/Rat

None

Polyclonal

Goat IgG

AF5665

100 µg

$355

Neuroglycan C/CSPG5

Neuroglycan C in Mouse Cerebellum. Neuroglycan C was detected in a section of adult mouse cerebellum using anti-mouse/rat Neuroglycan affinity-purified polyclonal antibody (Catalog # AF5665). The cells were stained using NorthernLights-557 anti-goat lgG secondary antibody (Catalog # NL001; red) and the nuclei counterstained with DAPI (blue).

Neuronal Pentraxin R

Human

None

Polyclonal

Sheep IgG

AF4414

100 µg

$355

Neurophysin II

Human

None

Polyclonal

Goat IgG

AF009

100 µg

$355

Human

None

Polyclonal

Sheep IgG

AF3870

100 µg

$355

None

Polyclonal

Goat IgG

AF566

100 µg

$355

None

130604

Mouse IgG1

MAB5661

500 µg

$325

Polyclonal

Goat IgG

AF2215

100 µg

$355

Neuropilin-1/BDCA4

Rat

Neuropilin-1 Expression in Mouse Embryonic Spinal Cord. Neuropilin-1 was detected in a cryostat section of mouse embryonic spinal cord (E15) using anti-rat Neuropilin-1 affinity-purified polyclonal antibody (Catalog # AF566). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Neuropilin-2

Human

None


79

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Neuroplastin Isoform 1

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Sheep IgG

AF5174

100 µg

$355

None

Polyclonal

Goat IgG

AF5360

100 µg

$355

None

Polyclonal

Goat IgG

AF1235

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1235

50 µg

$380

None

Polyclonal

Goat IgG

AF387

100 µg

$355

Neuroplastin in Human Cerebellum. Neuroplastin was detected in a paraffin-embedded human cerebellum section using anti-human Neuroplastin affinity-purified polyclonal antibody (Catalog # AF5174). The tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Neuroplastin 65

Human/Mouse

Neurotrimin

Human

Neurturin

Human Human/Mouse

None

Polyclonal

Goat IgG

AF477

100 µg

$355

NF2/Merlin aa 363-578

Human

None

Polyclonal

Goat IgG

AF5616

100 µg

$355

NFκB2 aa 1-447

Human

None

291319

Mouse IgG1

MAB28881

100 µg

$240

NG2/MCSP

Human

None

LHM-2

Mouse IgG1

MAB2585

100 µg

$240

Human

None

Polyclonal

Goat IgG

AF-256-NA

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF-556-NA

100 µg

$355

Human

None

74902

Mouse IgG1

MAB367

100 µg

$240

None

Polyclonal

Goat IgG

AF1157

100 µg

$355

b-NGF

NGF R/TNFRSF16

Mouse

Nicastrin Isoform 1

Human

Nidogen-1/Entactin

Human

Biotin

Polyclonal

Goat IgG

BAF1157

50 µg

$380

None

Polyclonal

Sheep IgG

AF5378

100 µg

$355

None

Polyclonal

Goat IgG

AF2570

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2570

50 µg

$380

None

302117

Mouse IgG1

MAB2570

100 µg

$240

None

Polyclonal

Goat IgG

AF3385

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF3385

50 µg

$380

AF5056

100 µg

$355

Nidogen-2

Human

Ninjurin-2

Human

None

Polyclonal

Sheep IgG

NKG2D/CD314

Mouse

Biotin

191004

Rat IgG2B

BAM1547

100 µg

$380

None

Polyclonal

Goat IgG

AF1850

100 µg

$355

None

195314

Mouse IgG2B

MAB1850

500 µg

$325

None

Polyclonal

Goat IgG

AF2444

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2444

50 µg

$380

None

259416

Mouse IgG1

MAB2444

100 µg

$240

NKp46/NCR1

Human

NKX2.5

Human

NKX6.1

Human/Mouse

None

Polyclonal

Goat IgG

AF5857

100 µg

$355

NMDA R, NR1 Subunit C1 Splice Variant

Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS080

25 µg

$355

NMDA R, NR1 Subunit C2 Splice Variant

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS082

25 µg

$355

Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS081

25 µg

$355

NMDA R, NR1 Subunit N1 Splice Variant

Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS083

25 µg

$355

NMDA R, NR2A Subunit

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS012

10 µg

$355

NMDA R, NR2B Subunit

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS013

10 µg

$355 $355

NMDA R, NR2C Subunit

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS033

10 µg

N-Me-6,7-diOH-TIQ

Multispecies

None

106814

Mouse IgG2B

MAB167

500 µg

$325

Noggin

Mouse

None

Polyclonal

Goat IgG

AF719

100 µg

$355

Nogo-A aa 2-172

Rat

None

Polyclonal

Goat IgG

AF3098

100 µg

$355

80


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Nogo-A aa 566-748

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Sheep IgG

AF3515

100 µg

$355

499825

Mouse IgG3

MAB5766

100 µg

$240

Nogo-A in differentiated rat cortical stem cells. Nogo-A was detected in 7-day differentiated rat cortical stem cells using anti-human Nogo-A affinitypurified polyclonal antibody (Catalog # AF3515). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

Nogo-C

Human

Norrin

Human

None None

Polyclonal

Goat IgG

AF3014

100 µg

$355

None

Polyclonal

Goat IgG

AF950

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF950

50 µg

$380

eNOS aa 1030-1203

Human

iNOS aa 781-798

Human

None

2D2-B2

Mouse IgG1

MAB9502

100 µg

$240

nNOS

Human

None

Polyclonal

Goat IgG

AF2416

100 µg

$355

Notch-1

Rat

None

Polyclonal

Goat IgG

AF1057

100 µg

$355

Notch-1 Intracellular Domain, aa 2428-2556

Human

None

433802

Mouse IgG2A

MAB3647

100 µg

$240

None

Polyclonal

Sheep IgG

AF3647

100 µg

$355

Polyclonal

Goat IgG

AF1190

100 µg

$355

Notch-1 in Human SAOS-2 Cells. Notch-1 was detected in human SAOS-2 osteosarcoma cells using anti-human Notch-1 affinity-purified polyclonal antibody (Catalog # AF3647). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

Notch-2

Rat

None

Notch-2 in Rat Brain. Notch-2 was detected in cryostat sections of rat brain (choroid plexus) using anti-rat Notch-2 affinity-purified polyclonal antibody (Catalog # AF1190). Tissues were stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Notch-2 Intracellular Domain

Human

Notch-3

Mouse

Notch-4 Intracellular Domain

Human

None

Polyclonal

Goat IgG

AF3735

100 µg

$355

None

Polyclonal

Goat IgG

AF1308

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1308

50 µg

$380

None

Polyclonal

Goat IgG

AF3847

100 µg

$355

None

411913

Rat IgG2A

MAB3847

100 µg

$240

None

Polyclonal

Goat IgG

AF1640

100 µg

$355 $380

NOV/CCN3

Human

Biotin

Polyclonal

Goat IgG

BAF1640

50 µg

NRAGE

Human

None

353124

Mouse IgG2B

MAB3835

100 µg

$240

NrCAM Isoform 3

Human

None

Polyclonal

Goat IgG

AF2034

100 µg

$355


81

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE NT-3

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Goat IgG

AF-267-NA

100 µg

$355

NT-3 in Rat Cerebellum. Neurotrophin-3 (NT-3) was detected in a cryostat section of rat cerebellar Purkinje neurons using anti-human NT-3 affinitypurified polyclonal antibody (Catalog # AF-267-NA). Tissues were stained with AEC substrate (red) and counterstained with hematoxylin (blue).

NT-4 Nucleostemin

Human

None

Polyclonal

Goat IgG

AF-268-NA

100 µg

$355

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF1638

100 µg

$355

Nucleostemin in Human U2OS Cells. Nucleostemin was detected in the nucleoli of the U2OS human osteosarcoma cell line using anti-human/mouse/ rat Nucleostemin affinity-purified polyclonal antibody (Catalog # AF1638). Cells were stained using NorthernLights-557 anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Numb

Nurr-1/NR4A2

None

Polyclonal

Sheep IgG

AF4338

100 µg

$355

Human

Biotin

Polyclonal

Sheep IgG

BAF4338

50 µg

$380

None

447716

Mouse IgG2A

MAB4338

100 µg

$240

Human

None

N1404

Mouse IgG2A

PP-N1404-00

100 µg

$380

Human/Mouse

None

Polyclonal

Goat IgG

AF2156

100 µg

$355

N-WASP

Human

None

Polyclonal

Goat IgG

AF3854

100 µg

$355

OAS2

Human

None

HLS56/3

Mouse N/A

MAB1925

100 µg

$240

OBCAM

Human

None

Polyclonal

Goat IgG

AF2777

100 µg

$355

None

Polyclonal

Goat IgG

AF1759

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1759

50 µg

$380

None

240408

Rat IgG2B

MAB1759

100 µg

$240

Oct-3/4

Human Human/Mouse

Oct-3/4 and Alkaline Phosphatase in Human BG01V Cells. Oct-3/4 was detected in BG01V embryonic stem cells using anti-human Oct-3/4 affinity-purified polyclonal antibody (Catalog # AF1759). Alkaline phosphatase (ALPL) was detected using anti-human/mouse/rat ALPL mono clonal antibody (Catalog # MAB1448). Cells were stained for Oct-3/4 using NorthernLights-637 donkey antigoat IgG secondary antibody (Catalog # NL002; red) and stained for ALPL using NorthernLights-557 donkey anti-mouse IgG secondary antibody (pseudo-stained green; Catalog # NL007).

Oct-3/4 NorthernLights-conjugated

Human/Mouse

Olig1

Human

Olig 1, 2, 3

Human

82

NL493

240408

Rat IgG2B

NL1759G

500 µL

$345

None

Polyclonal

Goat IgG

AF2417

100 µg

$355

None

257219

Mouse IgG2B

MAB2417

100 µg

$240

None

257224

Mouse IgG2A

MAB2230

100 µg

$295


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Olig2

SPECIES Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF2418

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2418

50 µg

$380

None

Polyclonal

Goat IgG

AF2356

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2356

50 µg

$380

None

257934

Mouse IgG1

MAB2456

100 µg

$240

Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC). Olig2 and O4 were detected in 7 day differentiated RCSC using anti-human Olig2 affinity-purified polyclonal antibody (Catalog # AF2418) and anti-human/mouse/rat/chicken O4 monoclonal antibody (Catalog # MAB1326). Cells were stained for Olig2 using NorthernLights-637 anti-goat IgG secondary antibody (Catalog # NL002; red) and for O4 using an anti-mouse IgM Secondary antibody (green).

Olig3

Human Human/Mouse

Oligodendrocyte Marker O1


None

O1

Mouse IgM

MAB1327

50 µg

$200

Oligodendrocyte Marker O4


None

O4

Mouse IgM

MAB1326

50 µg

$2040

NL637

O4

Mouse IgM

NL1326V

500 µL

$345

Biotin

97505

Mouse IgG1

BAM763

250 µg

$380

Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC). Oligodendrocyte marker O4 (O4) was detected in 7-day differentiated RCSC using anti-human/mouse/rat/chicken O4 monoclonal antibody (Catalog # MAB1326). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

Oligodendrocyte Marker O4 NorthernLights-conjugated


Orexin A

Human/Mouse/Rat

Orexin B

Human

None

97505

Mouse IgG1

MAB763

500 µg

$325

None

145202

Mouse IgG1

MAB734

500 µg

$325

$355

Orexin B, p38a, & Integrin b1 in Mouse Brain Stem. Orexin B, p38a, and Integrin b 1 were detected in frozen sections of mouse brain stem using antihuman Orexin B monoclonal antibody (Catalog # MAB734), anti-human/mouse/rat p38a affinity-purified polyclonal antibody (Catalog # AF8691), and anti-mouse Integrin b1 affinity-purified polyclonal antibody (Catalog # AF2405). Tissue was stained with NorthernLights (NL) secondary polyclonal antibodies: NL-493 anti-rabbit IgG (Catalog # NL006; green), NL-557 anti-mouse IgG (Catalog # NL007; red), and NL-637 anti-goat IgG (Catalog # NL002; blue).

Oncostatin M/OSM

OSM Rb

Mouse

None

Polyclonal

Goat IgG

AF-495-NA

100 µg

Human

None

469221

Mouse IgG1

MAB4389

100 µg

$240

None

Polyclonal

Goat IgG

AF662

100 µg

$355

Biotin

118125

Rat IgG2A

BAM662

100 µg

$240

None

118125

Rat IgG2A

MAB662

100 µg

$240

Mouse

Osteoactivin/GPNMB

Human

None

303822

Mouse IgG2B

MAB25501

100 µg

$240

Osteocalcin

Human

None

190125

Mouse IgG1

MAB1419

100 µg

$240

None

Polyclonal

Goat IgG

AF1433

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1433

50 µg

$380

None

190312

Mouse IgG2B

MAB1433

100 µg

$240

Osteopontin/OPN

Human

Mouse

Osteoprotegerin/TNFRSF11B

Human Mouse

None

Polyclonal

Goat IgG

AF808

100 µg

$355

None

Polyclonal

Goat IgG

AF805

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF805

50 µg

$380

Biotin

Polyclonal

Goat IgG

BAF459

50 µg

$380


83


SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF1979

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1979

50 µg

$380

Otx2

Human

None

246826

Mouse IgG2B

MAB1979

100 µg

$240

OV-6

Human/Rat

None

OV-6

Mouse IgG1

MAB2020

100 µg

$295

Human

None

159403

Mouse IgG1

MAB10541

500 µg

$325

OX40 Ligand/TNFSF4

p21/CIP1/CDKN1A

Mouse

Human

None

Polyclonal

Goat IgG

AF1236

100 µg

$355

None

Polyclonal

Goat IgG

AF1047

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1047

50 µg

$380

None

195720

Mouse IgG2A

MAB1047

200 µg

$240

CTS2256

p21 in Human Breast Cancer. p21 was detected in paraffin-embedded human breast cancer tissue sections using anti-human p21 affinity-purified polyclonal antibody (Catalog # AF1047). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with haematoxylin (blue).

p27/Kip1 Phospho-p38 MAP Kinase (T180/Y182)

Human

None

Polyclonal

50 Tests

$425

Human/Mouse/Rat

None

341116

Rat IgG2A

MAB22561

100 µg

$240

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF869

100 µg

$285

None

Polyclonal

Rabbit IgG

AF8691

100 µg

$285

None

142102

Mouse IgG2B

MAB869

100 µg

$240

Phosphorylated p38 in Embryonic Mouse Eye. Phosphorylated p38 was detected in a frozen section of E15 mouse eye cup using anti-phospho-p38 (T180/Y182) affinity-purified polyclonal antibody (Catalog # AF869). The tissue was stained using antirabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).

p38a

Human/Mouse/Rat

p38b

Human

None

280071

Mouse IgG2B

MAB3274

100 µg

$240

p38g

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF1347

100 µg

$285

p38d

Human

None

Polyclonal

Rabbit IgG

AF1519

100 µg

$285

Phospho-p53 (S15)

Human

None

Polyclonal

Rabbit IgG

AF1043

100 µg

$285

None

261366

Mouse IgG1

MAB18391

100 µg

$240

Phospho-p53 (S46)

Human

None

Polyclonal

Rabbit IgG

AF1489

100 µg

$285

None

Polyclonal

Goat IgG

AF1916

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1916

50 µg

$380

None

241631

Mouse IgG2B

MAB1916

100 µg

$240

None

Polyclonal

Rabbit IgG

AF8962

100 µg

$285

None

Polyclonal

Goat IgG

AF8964

100 µg

$355

None

215247

Mouse IgG1

MAB8962

100 µg

$240

None

Polyclonal

Goat IgG

AF896

100 µg

$285

p63/TP73L

p70 S6 Kinase

Phospho-p70 S6 Kinase (T229)

84

Human

Human/Mouse/Rat

Human


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE PAK7

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human/Rat

None

444703

Mouse IgG2B

MAB4696

100 µg

$240

Polyclonal

Goat IgG

AF3995

100 µg

$355

100 µg

$355

PAK7 in Human Cerebellum. p21-activated kinase 7 (PAK7) was detected in a paraffin-embedded human cerebellum section using anti-human PAK7 monoclonal antibody (Catalog # MAB4696). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Park7/DJ-1

Human

None

Parkin

Human

None

Polyclonal

Goat IgG

AF1438

PARP

Multi-species

None

C2-10

Mouse IgG1

4338-MC-50

Parvalbumin a

Human/Mouse/Rat

None

Polyclonal

Sheep IgG

413220

Polyclonal

50 µg

$195

AF5058

100 µg

$355

Rat IgG2A

MAB41051

100 µg

$240

Goat IgG

AF4078

100 µg

$355 $355

Parvalbumin a in Human Cortex. Parvalbumin a was detected in paraffin-embedded sections of human cortex using anti-human/mouse/rat Parvalbumin a monoclonal antibody (Catalog # AF5058). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Patched (First Extracellular Loop)

Mouse

None

Patched in Embryonic Mouse Spinal Cord. Patched was detected in ventral E13.5 mouse spinal cord using anti-mouse Patched monoclonal antibody (Catalog # MAB41051; red). Tissue was counterstained with DAPI (blue).

Patched 2 (First Extracellular Loop)

None

Human

None

Polyclonal

Goat IgG

AF2457

100 µg

Human/Mouse

None

274212

Mouse IgG2A

MAB2457

100 µg

$240

Pax4

Human

None

Polyclonal

Goat IgG

AF2614

100 µg

$355

Pax5

Human

Pax3

Pax6

Mouse/Rat/Chicken

Pax7


PBR

PD-1

PD-ECGF

PDGF-AA

PDGF-C

Human

None

Polyclonal

Goat IgG

AF3487

100 µg

$355

Biotin

PAX6

Mouse IgG1

BAM1260

100 µg

$260

None

PAX6

Mouse IgG1

MAB1260

100 µg

$240

None

PAX7

Mouse IgG1

MAB1675

100 µg

$240

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

6361-PC-100

100 µL

$310

Mouse/Rat

None

Polyclonal

Rabbit IgG

6362-PC-100

100 µL

$310

Human

None

Polyclonal

Goat IgG

AF1086

100 µg

$355

None

Polyclonal

Goat IgG

AF1021

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1021

50 µg

$380

None

Polyclonal

Goat IgG

AF1143

100 µg

$355

Mouse

Human

Human

Biotin

Polyclonal

Goat IgG

BAF1143

50 µg

$380

None

Polyclonal

Total Goat IgG

AB-221-NA

1 mg

$355

None

Polyclonal

Goat IgG

AF-221-NA

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF1055

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF1560

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF1447

100 µg

$355


85

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE PDGF-D

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF1159

100 µg

$355

None

Polyclonal

Goat IgG

AF-307-NA

100 µg

$355

None

35248

Mouse IgG1

MAB322

500 µg

$325

None

Polyclonal

Goat IgG

AF1062

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1062

50 µg

$380

Human PDGF Ra Mouse

SIZE

PRICE

Phospho-PDGF Ra (Y742)

Human

None

Polyclonal

Rabbit IgG

AF2114

50 µg

$285

Phospho-PDGF Ra (Y762)

Human

None

Polyclonal

Rabbit IgG

AF21141

50 µg

$285

None

Polyclonal

Goat IgG

AF385

100 µg

$355

None

PR7212

Mouse IgG1

MAB1263

250 µg

$230

None

Polyclonal

Goat IgG

AF1042

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1042

50 µg

$380

None

Polyclonal

Goat IgG

AF1224

100 µg

$355

None

176611

Mouse IgG2B

MAB1224

500 µg

$325

None

Polyclonal

Goat IgG

AF1022

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1022

50 µg

$380

None

Polyclonal

Goat IgG

AF2419

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2419

50 µg

$380

None

267712

Mouse IgG2B

MAB2419

100 µg

$240

Human PDGF Rb Mouse

PDGF Rb in Mouse Embryo. Platelet-derived Growth Factor Receptor b (PDGF Rb) was detected in a cryostat tissue section of E13 mouse spinal cord using anti-mouse PDGF Rb affinity-purified polyclonal antibody (Catalog # AF1042). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Human PD-L2 Mouse

PDX-1/IPF1

Human Human/Mouse

PDZK1

Human/Mouse

None

Polyclonal

Sheep IgG

AF4997

100 µg

$355

PEDF R

Human/Mouse/Rat

None

Polyclonal

Sheep IgG

AF5365

100 µg

$355

PER1 aa 159-365

Human

None

Polyclonal

Goat IgG

AF4565

100 µg

$355

Periostin/OSF-2

Mouse

None

345613

Rat IgG2A

MAB3548

100 µg

$240

Periostin/OSF-2 Isoform 2

Mouse

None

Polyclonal

Goat IgG

AF2955

100 µg

$355

Persephin

Human

None

275508

Mouse IgG2B

MAB2388

100 µg

$240

PIBF1

Human

None

Polyclonal

Sheep IgG

AF5559

100 µg

$355

pIgR

Mouse

None

Polyclonal

Goat IgG

AF2800

100 µg

$355

PIN

Human

None

Polyclonal

Goat IgG

AF877

100 µg

$355

Pin1 aa 2-163

Human/Mouse

None

257417

Mouse IgG2B

MAB2294

100 µg

$240

PINK1

Human

None

420920

Rat IgG2A

MAB4357

100 µg

$240

PKCb1

Human/Rat

None

Polyclonal

Goat IgG

AF3759

100 µg

$355

PKCι/λ PKC ι/λ/ζ Cross-reactive, aa 446-587

Human/Mouse/Rat

None

Polyclonal

Sheep IgG

AF4465

100 µg

$355

PKR

Human

None

HL71/10

Mouse IgG1

MAB1980

100 µg

$295

Plasminogen Kringle 5

Mouse

None

Polyclonal

Goat IgG

AF742

100 µg

$355

PLC-g2 aa 400-600

Human/Mouse

None

346404

Mouse IgG1

MAB3716

100 µg

$240

Plexin A1

Mouse

None

408305

Rat IgG2A

MAB4309

100 µg

$240

86


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Plexin A3

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Mouse

None

Polyclonal

Goat IgG

AF4075

100 µg

$355

None

Polyclonal

Goat IgG

AF3749

100 µg

$355

None

Polyclonal

Sheep IgG

AF5329

100 µg

$355

None

537237

Mouse IgG2A

MAB5329

100 µg

$240

Plexin A3 Expression in Embryonic Spinal Cord. Plexin A3 was detected in mouse embryonic (E13.5) spinal cord using anti-mouse Plexin A3 affinity-purified polyclonal antibody (Catalog # AF4075). Cells were stained using a NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Plexin B1

Human

Plexin B2

Human

Plexin B2 in Embryonic Mouse Choroid Plexus. Plexin B2 was detected in a section of embryonic (E13.5) mouse choroid plexus using anti-human Plexin B2 affinity-purified polyclonal antibody (Catalog # AF5329). The tissue was stained using antisheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and the nuclei counterstained with DAPI (blue).

PNR/NR2E3

Human

Human Podocalyxin Mouse

Plexin B2 in Human Colon Cancer. Plexin B2 was detected in paraffin-embedded human colon cancer sections using anti-human Plexin B2 affinity-purified polyclonal antibody (Catalog # AF5329). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown). The tissue was counterstained with hematoxylin (blue).

None

H7223

Mouse IgG2A

PP-H7223-00

100 µg

$380

None

Polyclonal

Goat IgG

AF1658

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1658

50 µg

$380

None

222328

Mouse IgG2A

MAB1658

100 µg

$240

None

Polyclonal

Goat IgG

AF1556

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1556

50 µg

$380

None

192703

Rat IgG2B

MAB1556

100 µg

$240

Podocalyxin in Human Kidney. Podocalyxin was detected in a paraffin-embedded human kidney tissue section using anti-human Podocalyxin affinity-purified polyclonal antibody (Catalog # AF1658). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Podocalyxin NorthernLights-conjugated Podocan

Podoplanin Potassium Channel Kv2.2

Podocalyxin in Human Embryonic Stem Cells (BGO1V). Podocalyxin was detected in the BGO1V human embryonic stem cell line cultured on mouse embryonic fibroblasts using anti-human Podocalyxin monoclonal antibody (Catalog # MAB1658). The cells were stained with anti-mouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).

Human

NL557

222328

Mouse IgG2A

NL1658R

500 µL

Human

None

390117

Mouse IgG2B

MAB42201

100 µg

$345 $240

Mouse

None

Polyclonal

Goat IgG

AF3104

100 µg

$355

Human

None

Polyclonal

Sheep IgG

AF3670

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF3244

100 µg

$355

Rat/Xenopus

None

Polyclonal

Rabbit IgG

PPS015

100 µL

$355

Phospho-Potassium Channel Kv3.1 (S503)

Mouse/Rat

None

Polyclonal

Rabbit IgG

PPS016

100 µL

$355

PP2A Catalytic Subunit, aa 209-309

Human

None

234532

Rat IgG2B

MAB16531

100 µg

$240

PP2Cg/PPM1G

Human

None

528322

Mouse IgG1

MAB5595

100 µg

$240

PPARg/NR1C3

Human

None

A3409A

Mouse IgG2A

PP-A3409A-00

100 µg

$380

None

Polyclonal

Goat IgG

AF166

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF166

50 µg

$380

None

Polyclonal

Goat IgG

AF149

100 µg

$355

None

121121

Mouse IgG2B

MAB149

500 µg

$325

Biotin

Polyclonal

Goat IgG

BAF197

50 µg

$380

Presenilin-1 C-Terminal Fragment, aa 298-407

Human

Presenilin-1 N-Terminal Fragment, aa 1- 80

Human

Presenilin-2 C-Terminal Fragment, aa 322-388

Human


87


LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Presenilin-2 N-Terminal Fragment, aa 1- 80

MOLECULE

Human

None

Polyclonal

Goat IgG

AF153

100 µg

$355

PRL/PTP4A Pan Specific

Human

None

334407

Mouse IgG2B

MAB32191

100 µg

$240

None

A9621A

Mouse IgG2A

PP-A9621A-00

100 µg

$380

None

1154

Mouse IgG1

PPS017

100 µL

$355

Human/Mouse/Rat/Primate

None

608

Mouse IgG1

PPS018

100 µL

$355

Human

None

H5344

Mouse IgG2A

PP-H5344-00

100 µg

$380

Progesterone R/NR3C3

Progesterone R/NR3C3 B

Human

Progesterone Receptor-B/NR3C3 in Human Endometrium. Progesterone Receptor isoform B was detected in paraffin-embedded human endometrial epithelial cells using anti-human Progesterone Receptor isoform B monoclonal antibody (Catalog # PPH5344-00). Tissues were stained with a 0.01% DAB solution (brown) and counterstained with hematoxylin (blue).

Human Progranulin

Mouse

Prohibitin

Human

Prolactin

Mouse

Prolactin R Prominin 2 Prostasin/Prss8

Human

None

Polyclonal

Goat IgG

AF2420

100 µg

$355

None

Polyclonal

Sheep IgG

AF2557

100 µg

$355

None

333731

Rat IgG2A

MAB25571

100 µg

$240

None

Polyclonal

Goat IgG

AF3470

100 µg

$355

None

Polyclonal

Goat IgG

AF1445

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1445

50 µg

$380

None

Polyclonal

Goat IgG

AF1167

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF1112

100 µg

$355

Human

None

244029

Mouse IgG2B

MAB2024

100 µg

$295

Human

None

Polyclonal

Sheep IgG

AF4599

100 µg

$355

Polyclonal

Sheep IgG

AF5064

100 µg

$355

Polyclonal

Goat IgG

AF5899

100 µg

$355

Prostasin in Human Prostate Cancer. Prostasin was detected in a paraffin-embedded human prostate cancer section using anti-human Prostasin affinity-purified polyclonal antibody (Catalog # AF4599). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). The section was stained using the antisheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Protocadherin a1

Human

None

Protocadherin a1 in Mouse Cerebellum. Proto cadherin a1 was detected in a section of adult mouse cerebellum using anti-mouse affinity-purified polyclonal antibody (Catalog # AF5064). The tissue was stained using anti-sheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and the nuclei counterstained with DAPI (blue).

Protocadherin-1

88

Human

None


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Protogenin

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Mouse

None

Polyclonal

Sheep IgG

AF4919

100 µg

$355

Polyclonal

Goat IgG

AF2727

100 µg

$355 $355

Protogenin in Embryonic Neural Tube. Protogenin (PRTG) was detected in a section of E9 mouse neural tube using anti-mouse PRTG affinity-purified polyclonal antibody (Catalog # AF4919). The tissue was stained using NorthernLights-557 anti-sheep polyclonal antibody (Catalog # NL101; red) and counterstained with DAPI (blue).

Prox1

Human

None

PSMA/FOLH1/NAALADase I

Human

None

Polyclonal

Sheep IgG

AF4234

100 µg

PTEN

Human/Mouse/Rat

None

217702

Mouse IgG1

MAB847

100 µg

$240

PTEN aa 385-403

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF847

100 µg

$285

PTH1 R

Human

None

Polyclonal

Sheep IgG

AF5709

100 µg

$355

PTP1B

Human

None

Polyclonal

Rabbit IgG

AF1366

100 µg

$285

PTP b/ζ aa 302-754

Human

None

279245

Rat IgG2A

MAB26881

100 µg

$240

PTP-MEG2

Human/Mouse/Rat

None

291835

Mouse IgG2B

MAB2668

100 µg

$240

PTPRg aa 111-541

Human

None

Polyclonal

Goat IgG

AF4608

100 µg

$355

Polyclonal

Goat IgG

AF3430

100 µg

$355

PTPRg in Human Kidney. Protein Tyrosine Phosphatase, Receptor type g (PTPRg) was detected in paraffin-embedded normal human kidney tissue sections using anti-human PTPRg affinity-purified polyclonal antibody (Catalog # AF4608). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

PTPRσ aa 30-1260

Human

None

RACK1

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF3434

100 µg

$355

Rae-1g

Mouse

None

Polyclonal

Goat IgG

AF1136

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF1145

100 µg

$355

RAGE

Mouse

None

Polyclonal

Goat IgG

AF1179

100 µg

$355

Mouse/Rat

None

175410

Rat IgG2A

MAB1179

500 µg

$325

80707

Mouse IgG2A

MAB6831

500 µg

$325

RAGE Expression in Human Alzheimer’s Brain. Receptor for Advanced Glycation End products (RAGE) was detected in paraffin-embedded Alzheimer’s disease human cerebellum sections using anti-human RAGE affinity-purified polyclonal antibody (Catalog # AF1145). Tissue was stained using the anti-goat HRPDAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

RANK/TNFRSF11A

Human

None

Mouse

None

Polyclonal

Goat IgG

AF692

100 µg

$355

Rap1A/B

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF3767

100 µg

$355

RARRES1

Mouse

None

Polyclonal

Goat IgG

AF4657

100 µg

$355

Reelin

Mouse

None

Polyclonal

Goat IgG

AF3820

100 µg

$355

Reg II

Mouse

None

Polyclonal

Goat IgG

AF2035

100 µg

$355

Reg 2/PAP

Rat

None

293523

Mouse IgG1

MAB1996

100 µg

$240


89

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Reg IIIb

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Mouse

None

Polyclonal

Sheep IgG

AF5110

100 µg

$355

518818

Rat IgG2A

MAB5678

100 µg

$240

Reg IIIb in Mouse Small Intestine. Reg IIIb was detected in adult mouse small intestine sections using anti-mouse Reg IIIb affinity-purified polyclonal antibody (Catalog # AF5110). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

Reg IIId

Mouse

None

Reg IV

Human

None

Polyclonal

Goat IgG

AF1379

100 µg

$355

RelB aa 382-579

Human

None

315206

Mouse IgG2B

MAB2698

100 µg

$240

321422

Rat IgG2A

MAB4606

50 µg

$240

RelB in Human Lymphoma. RelB was detected in a paraffin-embedded human lymphoma section using anti-human RelB monoclonal antibody (Catalog # MAB2698). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

c-Rel c-Rel aa 1-588

Human

None

Human/Mouse

None

Polyclonal

Goat IgG

AF2699

100 µg

$355

Mouse

Biotin

290512

Rat IgG2A

BAM2699

100 µg

$380

c-Rel in Mouse Splenocytes. c-Rel was detected in mouse splenocytes using anti-mouse c-Rel biotinylated monoclonal antibody (Catalog # BAM2699).Cells were stained using NorthernLights Streptavidin-557 (Catalog # NL999; red) and counterstained with DAPI (blue)..

Renin

Human

None

Polyclonal

Sheep IgG

AF4090

100 µg

$355

Renin 1

Mouse

None

Polyclonal

Goat IgG

AF4277

100 µg

$355

Renin 1 Propeptide

Mouse

None

434703

Rat IgG2A

MAB42771

100 µg

$240

Renin Propeptide

Human

None

411507

Mouse IgG1

MAB4447

100 µg

$240

Resistin

Mouse

None

Polyclonal

Goat IgG

AF1069

100 µg

$355

Ret REVERBb/NR1D2

Rex-1

Human

None

Polyclonal

Goat IgG

AF1485

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF482

100 µg

$355

Human

Human

None

H2729

Mouse IgG3

PP-H2729-00

100 µg

$380

None

Polyclonal

Sheep IgG

AF3598

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF3598

50 µg

$380

None

373722

Rat IgG2A

MAB3598

100 µg

$240

Rex-1 in Human NTera2 Cells. Rex-1 was detected in human NTera2 embryonic carcinoma cell line using antihuman monoclonal antibody (Catalog # MAB3598). Cells were stained using NorthernLights-557 goat antirat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).

90



Repulsive Guidance Molecule A/RGM-A

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF2459

100 µg

$355

None

Polyclonal

Goat IgG

AF2458

100 µg

$355

Mouse

RGM-B

SIZE

PRICE

Biotin

Polyclonal

Goat IgG

BAF2458

50 µg

$380

Human/Mouse

None

Polyclonal

Sheep IgG

AF3597

100 µg

$355

Mouse

Biotin

Polyclonal

Sheep IgG

BAF3597

50 µg

$380

RGM-B in Mouse Brain. Repulsive Guidance Molecule B (RGM-B) was detected in a cryostat section of mouse brain (trigeminal ganglia) using anti-mouse RGM-B affinity-purified polyclonal antibody (Catalog # AF3597). Tissues were stained using anti-sheep HRP-DAB detection (brown) and counterstained with hematoxylin (blue).

Rheb

Human/Mouse/Rat

None

344912

Mouse IgG2B

MAB3426

100 µg

$240

Phospho-Ribosomal Protein S6 (S235/S236)

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF3918

100 µg

$285

None

Polyclonal

Goat IgG

AF1749

100 µg

$355

None

Polyclonal

Goat IgG

AF3147

100 µg

$355

None

356001

Mouse IgG1

MAB3147

100 µg

$240

None

Polyclonal

Goat IgG

AF3076

100 µg

$355 $240

Phospho-RPS6 in Human Colon Cancer. PhosphoRibosomal Protein S6 (S235/S236) was detected in paraffin-embedded human colon cancer tissue sections using anti-human/mouse/rat affinity-purified phospho-RPS6 (S235/S236) affinity-purified polyclonal antibody (Catalog # AF3918). The tissue was stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).

ROBO1

Rat

ROBO2

Human

ROBO3

Human

None

327111

Mouse IgG2B

MAB3076

100 µg

ROR/NR1F1-3 Pan Specific

Human

None

H3925

Mouse IgG2A

PP-H3925-00

100 µg

$380

Phospho-RSK (S380) Pan Specific

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF889

100 µg

$285

Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227)

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF892

100 µg

$285

RSK1

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF992

100 µg

$285

RSK2

Human/Mouse/Rat

None

Polyclonal

Rabbit IgG

AF1518

50 µg

$285

Phospho-RSK3 (S218) RUNX2/CBFA1

Human/Mouse

None

Polyclonal

Rabbit IgG

AF893

100 µg

$285

Human

None

Polyclonal

Goat IgG

AF2006

100 µg

$355

Human/Mouse

None

232902

Rat IgG2B

MAB2006

100 µg

$240

RUNX3/CBFA3 aa 186-415

Human

None

Polyclonal

Goat IgG

AF3765

100 µg

$355

RXRa/NR2B1

Human

None

K8508

Mouse IgG2A

PP-K8508-00

100 µg

$380

None

K8508

Mouse IgG2A

PP-K8508-10

100 µg

$380

Ryk

Mouse

None

Polyclonal

Sheep IgG

AF4649

100 µg

$355

S100A2

Human

None

Polyclonal

Goat IgG

AF4870

100 µg

$355

S100A4

Mouse

None

438709

Rat IgG2B

MAB4138

100 µg

$240


91


S100A8

SPECIES

Mouse

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF3059

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF3059

50 µg

$380

None

335806

Rat IgG2B

MAB3059

100 µg

$240

S100A8 in Mouse NIH-3T3 Cells. S100A8 was detected in mouse NIH-3T3 embryonic fibroblast cell line using anti-mouse S100A8 monoclonal antibody (Catalog # MAB3059; red). Cells were counterstained with DAPI (blue).

S100A9

Human

None

474315

Mouse IgG2B

MAB5578

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF2065

100 µg

$355

S100A9 in Mouse XB2 Cells. S100A9 was detected in mouse XB2 teratoma cell line using anti-mouse S100A9 affinity-purified polyclonal antibody (Catalog # AF2065). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Human S100A10

Mouse

None

Polyclonal

Goat IgG

AF1698

100 µg

$355

None

Polyclonal

Goat IgG

AF2377

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2377

50 µg

$380

None

344015

Rat IgG2A

MAB2377

100 µg

$240

S100A11

Human

None

Polyclonal

Goat IgG

AF4874

100 µg

$355

S100A13

Mouse

None

Polyclonal

Goat IgG

AF4328

100 µg

$355

Polyclonal

Sheep IgG

AF5180

100 µg

$355

Polyclonal

Goat IgG

AF1820

100 µg

$355

S100A13 in Embryonic Mouse Cartilage. S100A13 was detected in a cryostat section of developing embryonic mouse (E13) cartilage using anti-mouse S100A13 affinity-purified polyclonal antibody (Catalog # AF4328). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

S100A16

Human

None

S100A16 in Human Brain Cortex. S100A16 was detected in a paraffin-embedded human brain cortex section using anti-human S100A16 affinity-purified polyclonal antibody (Catalog # AF5180). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). The section was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

S100B

92

Human

None


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE S100P

SPECIES Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF2957

100 µg

$355

None

357517

Mouse IgG1

MAB2957

100 µg

$240

S100P in Human Liver. S100P was detected in paraffin-embedded human liver sections using antihuman S100P affinity-purified polyclonal antibody (Catalog # AF2957). Tissue was stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

SALL1 aa 258-499

Human

None

K9814

Mouse IgG2A

PP-K9814-00

100 µg

$380

d-Sarcoglycan

Human

None

Polyclonal

Goat IgG

AF2778

100 µg

$355

None

Polyclonal

Goat IgG

AF1226

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1226

50 µg

$380

Biotin

177228

Rat IgG2A

BAM1226

250 µg

$380

None

177228

Rat IgG2A

MAB1226

500 µg

$325

Mouse

None

462002

Rat IgG2A

MAB4754

100 µg

$240

Canine

None

Polyclonal

Goat IgG

AF2278

100 µg

$355

Feline

None

Polyclonal

Goat IgG

AF2268

100 µg

$355

Sca-1/Ly6

SCARA5 SCF/c-kit Ligand Phospho-SCF R/c-kit (Y730)

SCF R/c-kit

Mouse

Human

None

Polyclonal

Rabbit IgG

AF3527

50 µg

$285

Human

None

Polyclonal

Goat IgG

AF332

100 µg

$355

None

Polyclonal

Goat IgG

AF1356

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1356

50 µg

$380

Biotin

180627

Rat IgG2A

BAM1356

250 µg

$380

None

180627

Rat IgG2A

MAB1356

500 µg

$325

50 µg

$285

Mouse

SCF R in Mouse Cerebellum. Stem Cell Factor Receptor (SCF R) was detected in frozen sections of mouse cerebellum using anti-mouse SCF R monoclonal antibody (Catalog # MAB1356). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

SCF R/c-kit aa 716-726

Human

None

Polyclonal

Rabbit IgG

AF3267

SCGF/CLEC11a

Human

None

239029

Mouse IgG2A

MAB1904

500 µg

$325

SCL/Tal1

Human

None

Polyclonal

Goat IgG

AF3360

100 µg

$355

SCL/Tal1

Human

None

333803

Mouse IgG2B

MAB3360

100 µg

$240

Human SDNSF/MCFD2

Mouse

Human E-Selectin/CD62E Mouse Rat L-Selectin/CD62L

Rat

None

294301

Mouse IgG2B

MAB2357

100 µg

$240

None

Polyclonal

Goat IgG

AF2556

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2556

50 µg

$380

None

300812

Rat IgG2A

MAB2556

100 µg

$240

None

BBIG-E6

Mouse IgG1

BBA1

100 µg

$230

None

BBIG-E4

Mouse IgG1

BBA16

200 µg

$230

None

Polyclonal

Goat Serum

BBA18

500 µL

$195

None

BBIG-E4

Mouse IgG1

BBA2

None

Polyclonal

Chicken IgY

AF575

1 mg

$840

100 µg

$355

Biotin

Polyclonal

Chicken IgY

BAF575

50 µg

$380

None

Polyclonal

Goat IgG

AF977

100 µg

$355

None

Polyclonal

Goat IgG

AF1534

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1534

50 µg

$380


93

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE P-Selectin/CD62P

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

9.00E+01

Mouse IgG1

BBA30

200 µg

SIZE

PRICE $315

None

Polyclonal

Sheep IgG

AF1728

100 µg

$355

Semaphorin 3C

Mouse

Biotin

Polyclonal

Sheep IgG

BAF1728

50 µg

$380

Semaphorin 3E

Human

None

Polyclonal

Goat IgG

AF3239

100 µg

$355

Semaphorin 4D/CD100

Mouse

None

Polyclonal

Sheep IgG

AF5235

100 µg

$355

Human Semaphorin 6A

Mouse

Semaphorin 6B

Human

Semaphorin 6C

Mouse

Serpin A4/Kallistatin

Human

None

Polyclonal

Goat IgG

AF1146

100 µg

$355

None

Polyclonal

Goat IgG

AF1615

100 µg

$355

None

201932

Rat IgG2A

MAB1615

500 µg

$325

None

Polyclonal

Goat IgG

AF2094

100 µg

$355

None

Polyclonal

Goat IgG

AF2108

100 µg

$355

None

256631

Rat IgG2A

MAB2108

500 µg

$325

None

Polyclonal

Goat IgG

AF1669

100 µg

$355

Serpin A6

Mouse

None

Polyclonal

Goat IgG

AF4065

100 µg

$355

Serpin A8/Angiotensinogen

Human

None

Polyclonal

Goat IgG

AF3156

100 µg

$355

Serpin E1/PAI-1

Human

None

Polyclonal

Goat IgG

AF1786

100 µg

$355

Serpin F1/PEDF

Human

None

Polyclonal

Goat IgG

AF1177

100 µg

$355

None

187003

Mouse IgG2B

MAB1177

500 µg

$325

None

Polyclonal

Goat IgG

AF1239

100 µg

$355

Serpin F2/a2-Antiplasmin

Mouse

Biotin

Polyclonal

Goat IgG

BAF1239

50 µg

$380

Serum Amyloid A1

Mouse

None

Polyclonal

Goat IgG

AF2948

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF3856

100 µg

$355

Serum Amyloid A4

Serum Amyloid A4 in Human Liver. Serum amyloid A4 (SAA4) was detected in paraffin-embedded human liver tissue sections using anti-human serum amyloid A4 affinity-purified polyclonal antibody (Catalog # AF3856) and Antigen Retrieval Reagent - Basic (Catalog # CTS013). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

SF-1/NR5A1

Human

None

N1665

Mouse IgG1

PP-N1665-00

100 µg

$380

None

Polyclonal

Goat IgG

AF592

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF592

50 µg

$380

None

Polyclonal

Goat IgG

AF2656

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2656

50 µg

$380

257812

Rat IgG2A

MAB2317

100 µg

$240

sFRP-3

Mouse

SHBG

Human

SHIP aa 874-941

Human/Mouse/Rat

None

Human

None

255402

Rat IgG2A

MAB1878

100 µg

$240

Human/Mouse/Rat

None

427043

Mouse IgG2B

MAB18781

100 µg

$240

AF3790

100 µg

$285

SHP-1 aa 205-595

SHP-1 in Human Thymus. Src-Homology 2 domain Phosphatase-1 (SHP-1) was detected in a paraffinembedded human thymus section using antihuman/mouse/rat SHP-1 monoclonal antibody (Catalog # MAB18781). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Phospho-SHP-2 (Y542) Siglec-2/CD22 Siglec-5

94

Human/Mouse

None

Polyclonal

Rabbit IgG

Human

None

219934

Mouse IgG1

MAB1968

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF2296

100 µg

$355

Human

None

194117

Mouse IgG2B

MAB1072

500 µg

$325


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Siglec-5/14 Siglec-7

Siglec-9

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

Human

None

Polyclonal

Goat IgG

AF1072

100 µg

$355

None

Polyclonal

Goat IgG

AF1138

100 µg

$355

None

194212

Mouse IgG2B

MAB1138

500 µg

$325

None

Polyclonal

Goat IgG

AF1139

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1139

50 µg

$380

Human

Human

SIZE

PRICE

SKI

Human

None

Polyclonal

Goat IgG

AF4025

100 µg

$355

SLAIN1 aa 262-483

Human/Mouse

None

Polyclonal

Goat IgG

AF5638

100 µg

$355

Polyclonal

Goat IgG

AF2798

100 µg

$355

SLAIN1 in Mouse D3 Cells. SLAIN1 was detected in the D3 mouse embryonic stem cell line using antihuman/mouse SLAIN1 affinity-purified polyclonal antibody (Catalog # AF5638). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

Sleeping Beauty Transposase

Multi-species

SLITRK1

Human

SLPI

Human

None None

324622

Mouse IgG1

MAB2798

100 µg

$240

None

327829

Mouse IgG2B

MAB30091

100 µg

$240

None

Polyclonal

Total Goat IgG

AB-260-NA

1 mg

$355

None

Polyclonal

Goat IgG

AF1274

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1274

50 µg

$380

SMAC/Diablo

Human

None

Polyclonal

Goat IgG

AF7891

100 µg

$355

Smad1 aa 2-454

Human

None

Polyclonal

Goat IgG

AF2039

100 µg

$355

Smad2 aa 20-108

Human

None

376520

Mouse IgG2B

MAB4037

100 µg

$240

Smad3 aa 2-230

Human

None

378611

Rat IgG1

MAB4038

100 µg

$240

$355

Smad3 in Human Pancreatic Cancer. Smad3 was detected in paraffin-embedded human pancreatic cancer sections using anti-human Smad3 monoclonal antibody (Catalog # MAB4038). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissue was counterstained with hematoxylin (blue).

Smad4 aa 139-332

Human

None

Polyclonal

Goat IgG

AF2097

100 µg

Smad4 aa 139-332

Human

None

253343

Mouse IgG2B

MAB2097

100 µg

$240

Smad5 aa 155-258

Human

None

Polyclonal

Goat IgG

AF2250

100 µg

$355

Smad5 in Human Colon. Smad5 was detected in paraffin-embedded human colon tissue sections using anti-human Smad5 affinity-purified polyclonal antibody (Catalog # AF2250). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).


95

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Smad7 aa 320-398

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human/Mouse/Rat

None

293039

Mouse IgG2B

MAB2029

100 µg

$240

Polyclonal

Goat IgG

AF2309

100 µg

$355

Smad7 in Human Liver Cancer. Smad7 was detected in paraffin-embedded human liver cancer sections using anti-human/mouse/rat Smad7 monoclonal antibody (Catalog # MAB2029). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

Smad8 aa 158-223 SMAGP

Human

None

Human

None

Polyclonal

Goat IgG

AF3959

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF3967

100 µg

$355

1A4

Mouse IgG2A

MAB1420

100 µg

$240

SMAGP in Human Colon. Small Transmembrane and Glycosylated Protein (SMAGP) was detected in paraffin-embedded human colon sections using antihuman SMAGP affinity-purified polyclonal antibody (Catalog # AF3959). Tissue was stained using the antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

a-Smooth Muscle Actin N-terminal Synthetic Decapeptide

Human

None

Snail

Human

None

Polyclonal

Goat IgG

AF3639

100 µg

$355

SNF1LK2

Human

None

Polyclonal

Goat IgG

AF5737

100 µg

$355

SOCS-3 aa 1-225

Human/Mouse

None

516919

Mouse IgG2B

MAB5696

100 µg

$240

SOCS-5

Human

None

Polyclonal

Goat IgG

AF4796

100 µg

$355

SOD1/Cu-Zn SOD

Human

None

348808

Mouse IgG2A

MAB3418

100 µg

$240

SOD2/Mn-SOD aa 25-222

Human/Mouse/Rat

None

349810

Mouse IgG1

MAB3419

100 µg

$240

Somatostatin R2/SSTR2

Human

None

402038

Mouse IgG2A

MAB4224

100 µg

$240

Sonic Hedgehog C-Terminus

Mouse

Sonic Hedgehog N-Terminus

Mouse

None

Polyclonal

Goat IgG

AF445

100 µg

$355

None

Polyclonal

Goat IgG

AF464

100 µg

$355

None

171018

Rat IgG2A

MAB4641

500 µg

$325

SorCS1 aa 111-1099

Human

None

Polyclonal

Goat IgG

AF3457

100 µg

$355

SorCS2

Mouse

None

Polyclonal

Sheep IgG

AF4237

100 µg

$355

SorCS3

Mouse

None

Polyclonal

Goat IgG

AF3067

100 µg

$355

SorCS3 aa 34-1122

Mouse

None

339615

Rat IgG2A

MAB30671

100 µg

$240

SorLA

Human

None

525122

Mouse IgG1

MAB5699

100 µg

$240

Human

None

Polyclonal

Goat IgG

AF3154

100 µg

$355

Sortilin

Mouse

SOST/Sclerostin

Mouse

SOX1

Human

SOX2

Human Human/Mouse

96

None

Polyclonal

Goat IgG

AF2934

100 µg

$355

None

Polyclonal

Goat IgG

AF1589

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1589

50 µg

$380

None

Polyclonal

Goat IgG

AF3369

100 µg

$355

None

Polyclonal

Goat IgG

AF2018

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2018

50 µg

$380

None

245610

Mouse IgG2A

MAB2018

100 µg

$240



SPECIES

SOX2 NorthernLights-conjugated

Human/Mouse

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

NL493

245610

Mouse IgG2A

NL2018G

500 µL

$345

NL557

245610

Mouse IgG2A

NL2018R

500 µL

$345

NL637

245610

Mouse IgG2A

NL2018V

500 µL

$345

None

Polyclonal

Goat IgG

AF2569

100 µg

$355

None

287403

Mouse IgG2A

MAB2569

100 µg

$240

SOX2 in Human BG01V Cells. SOX2 was detected in the human BG01V embryonic stem cell line using anti-human/mouse SOX2 NorthernLights-637-conjugated monoclonal antibody (Catalog # NL2018V; red). Cells were counterstained with DAPI (blue). The human BG01V embryonic stem cell line was grown on Irradiated Mouse Embryonic Fibroblasts (iMEF; Catalog # PSC001).

SOX3

Human

SOX7

Human

SOX9

Human

None

Polyclonal

Goat IgG

AF2766

100 µg

$355

None

Polyclonal

Goat IgG

AF3075

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF3075

50 µg

$380

SOX9 in Human HEK293 Cells. SOX9 was detected in human HEK293 embryonic kidney cell line using anti-human SOX9 affinity-purified polyclonal antibody (Catalog # AF3075). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).


Human

NL557

Polyclonal

Goat IgG

NL3075R

500 µL

$350

Human

None

Polyclonal

Goat IgG

AF2864

100 µg

$355

Human/Rat

None

20B7

Mouse IgG1

MAB2864

100 µg

$240


Human/Rat

NL557

20B7

Mouse IgG1

NL2864R

500 µL

$345

SOX15

Human

None

Polyclonal

Sheep IgG

AF4070

100 µg

$355

None

Polyclonal

Goat IgG

AF1924

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1924

50 µg

$380

None

245013

Mouse IgG3

MAB1924

100 µg

$240

NL557

Polyclonal

Goat IgG

NL1924R

500 µL

$350

SOX10

SOX17

Human

SOX17 NorthernLights-conjugated, aa 177-144

Human

SOX17 in Mouse Melanoma Cells. SOX17 was detected in the B16 mouse melanoma cell line using anti-human SOX17 affinity-purified polyclonal antibody (Catalog # AF1924). The cells were stained with NorthernLights-557 anti goat secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

SOX21

SPARC/Osteonectin

Spinesin

Phospho-Src (Y419)

Human Human

None

Polyclonal

Goat IgG

AF3538

100 µg

$355

None

Polyclonal

Goat IgG

AF941

100 µg

$355

None

122511

Mouse IgG1

MAB941

500 µg

$325

Mouse

None

124413

Rat IgG2B

MAB942

500 µg

$325

Human

None

Polyclonal

Goat IgG

AF2495

100 µg

$355

None

Polyclonal

Goat IgG

AF1928

100 µg

$355

Mouse Human

Biotin

Polyclonal

Goat IgG

BAF1928

50 µg

$380

None

Polyclonal

Rabbit IgG

AF2685

100 µg

$285


97


LABEL

CLONE

HOST/TYPE

CATALOG #

Src

MOLECULE

Human

None

327554

Mouse IgG2A

MAB3807

100 µg

SIZE

PRICE $240

Src aa 1-79

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF3389

100 µg

$355

Src in Human Colon. Src was detected in paraffinembedded normal human colon sections using antihuman/rat/mouse Src affinity-purified polyclon al antibody (Catalog # AF3389). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

SSEA-1

Human/Mouse

None

MC-480

Mouse IgM

MAB2155

100 µg

$240

SSEA-1 NorthernLights-conjugated

Human/Mouse

NL557

MC-480

Mouse IgM

NL2155R

500 µL

$345

SSEA-3

Human/Mouse

None

MC-631

Rat IgM

MAB1434

100 µg

$230

SSEA-4

Human/Mouse

Biotin

MC-813-70

Mouse IgG3

BAM1435

100 µg

$295

None

MC-813-70

Mouse IgG3

MAB1435

100 µg

$230

STAT1

Human

None

246523

Rat IgG2B

MAB1490

100 µg

$240

STAT2

Human

None

545103

Mouse IgG2B

MAB16661

100 µg

$240

Phospho-STAT3 (Y705)

Human

None

Polyclonal

Rabbit IgG

AF4607

100 µg

$285

Polyclonal

Sheep IgG

AF17991

100 µg

$355

Phospho-STAT3 in Human Daudi Cells. PhosphoSignal Transducer and Activator of Transcription 3 (STAT3) was detected in human INF-a-treated Daudi human cells (Y705) treated with IFN-a using antihuman phospho-STAT3 affinity-purified polyclonal antibody (Catalog # AF4607). Cells were stained using NorthernLights-557 donkey anti-rabbit IgG secondary antibody (Catalog # NL004; red) and counterstained with DAPI (blue).

None

STAT3 aa 1-175

Human

None

232209

Mouse IgG2B

MAB1799

100 µg

$240

STAT3 aa 688-770

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF1799

100 µg

$355

STAT3 NorthernLights-conjugated

Human/Mouse/Rat

NL557

232209

Mouse IgG2B

NL1799R

500 µL

$345

Human

None

Polyclonal

Rabbit IgG

AF4190

100 µg

$285

Phospho-STAT5a/b (Y699)

Phospho-STAT5 (Y699) in IFN-α-treated Daudi cells. Phospho-Signal Transducer and Activator of Transcription 5 (Y699) was detected in IFN-a treated Daudi BurKitt’s lymphoma human cells using antihuman/mouse phosphor-STAT5 (Y699) polyclonal antibody (Catalog # AF4190). The cells were stained using NorthernLights-557 anti-rabbit secondary antibody (Catalog # NL004; red) and counterstained with DAPI (blue).

STAT5a aa 774-794 STAT6 aa 342-640

Human

None

251610

Mouse IgG1

MAB21741

100 µg

$240

Human/Mouse

None

251619

Mouse IgG3

MAB2174

100 µg

$240

Human

None

253906

Mouse IgG2A

MAB2167

100 µg

$240

STAT6 in Human Peripheral Blood Mononuclear Cells (PBMCs). Signal Transducer and Activator of Transcription 6 (STAT6) was detected in PHA-stimulated human PBMCs using anti-human/mouse STAT6 monoclonal antibody (Catalog # MAB2167; red). Cells were counterstained (green).

98



SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF2566

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2566

50 µg

$380 $240

Stella/Dppa3

Mouse

None

283910

Rat IgG2A

MAB2566

100 µg

STRA6

Human

None

496613

Mouse IgG1

MAB5904

100 µg

$240

STRO-1

Human

None

STRO-1

Mouse IgM

MAB1038

100 µg

$295

Substance P

Human/Mouse/Rat

None

266815

Mouse IgG1

MAB4375

100 µg

$240

SUMO3

Human

None

401513

Rat IgG2A

MAB2959

100 µg

$240

Survivin

Human

None

Polyclonal

Rabbit IgG

AF886

100 µg

$285

SUZ12

Human

None

448909

Mouse IgG1

MAB4184

100 µg

$240

Synapsin I Pan Specific

Human/Mouse/Rat/Bovine

None

Polyclonal

Rabbit IgG

PPS035

10 µg

$355

Synaptophysin

Human

None

Polyclonal

Goat IgG

AF5555

100 µg

$355

Synaptotagmin

Rat

None

ASV48

Mouse IgG2B

MAB4364

100 µg

$240

None

ASV30

Mouse IgG2A

MAB43641

100 µg

$240

Synaptotagmin I in Rat Spinal Cord. Synapto tagmin I was detected in a cryostat section of rat spinal cord using anti-rat Synaptotagmin I monoclonal antibody (Catalog # MAB43641; red). The tissue was counterstained green.

Syndecan-1/CD138

Human Human

Syndecan-1/CD138 Mouse

Syndecan-2

Syndecan-3

Human Human

Polyclonal

Goat IgG

AF2780

100 µg

$355

Polyclonal

Goat IgG

BAF2780

50 µg

$380

None

359103

Rat IgG1

MAB2780

100 µg

$240

None

Polyclonal

Goat IgG

AF3190

100 µg

$355

None

300506

Rat IgG1

MAB2966

100 µg

$240

None

Polyclonal

Goat IgG

AF2965

100 µg

$355

None

305507

Rat IgG2A

MAB29651

100 µg

$240

None

374412

Rat IgG2A

MAB35391

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF2734

100 µg

$355

Syndecan-3 Isoform 1

Human

None

Polyclonal

Goat IgG

AF3539

100 µg

$355

Syndecan-4

Human

None

Polyclonal

Goat IgG

AF2918

100 µg

$355

Syntaxin-BP1

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF5675

100 µg

$355

Synuclein-a

Human

None

Polyclonal

Goat IgG

AF1338

100 µg

$355

Synuclein-b

Human

None

Polyclonal

Sheep IgG

AF5528

100 µg

$355

None

516505

Mouse IgG2B

MAB5528

100 µg

$240

Synuclein-g

Human

None

514304

Mouse IgG1

MAB5745

100 µg

$240

TAB1 aa 7-159

Human/Mouse

None

Polyclonal

Goat IgG

AF3578

100 µg

$355

TACE/ADAM17 Cytosolic Domain

Human

None

Polyclonal

Goat IgG

AF2129

100 µg

$355

TACE/ADAM17 Ectodomain

Human

None

Polyclonal

Chicken IgY

AF930

100 µg

$355

Biotin

Polyclonal

Chicken IgY

BAF930

50 µg

$380

None

Polyclonal

Goat IgG

AF174

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF174

50 µg

$380

None

165609

Mouse IgG1

MAB174

500 µg

$325

None

Polyclonal

Goat IgG

AF1041

100 µg

$355

Human TACI/TNFRSF13B Mouse

None Biotin

Biotin

Polyclonal

Goat IgG

BAF1041

50 µg

$380

Tau

Multi-species

None

Polyclonal

Goat IgG

AF3494

100 µg

$355

T-bet/TBX21

Human

None

525803

Mouse IgG1

MAB5385

100 µg

$240

TBX2

Human

None

393720

Rat IgG2A

MAB50401

100 µg

$240

TBX3 aa 321-483

Human

None

Polyclonal

Goat IgG

AF4509

100 µg

$355


99

R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE TBX6

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

Polyclonal

Goat IgG

AF4744

100 µg

$355

Polyclonal

Goat IgG

AF3605

100 µg

$355

TBX6 in Mouse Embryonic Mesoderm. T-box Protein 6 (TBX6) was detected in E9.5 mouse embryonic tailbud presomitic mesoderm sections using antihuman TBX6 affinity-purified polyclonal antibody (Catalog # AF4744). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

TC21/R-Ras2

Human/Rat

None

TCCR/WSX-1

Human

None

Polyclonal

Goat IgG

AF1479

100 µg

$355

TCF-2/HNF-1b

Human

None

Polyclonal

Goat IgG

AF3330

100 µg

$355

TCF-7/TCF-1

Human

None

Polyclonal

Goat IgG

AF5596

100 µg

$355

TCL1A

Human

None

Polyclonal

Goat IgG

AF4847

100 µg

$355

TEM8 Isoform 1

Human

None

Polyclonal

Goat IgG

AF3886

100 µg

$355

None

Polyclonal

Goat IgG

AF3358

100 µg

$355 $240

Tenascin C

Tenascin R

Human

None

391819

Rat IgG2A

MAB3358

100 µg

Human/Mouse

None

578

Rat IgG2A

MAB2138

100 µg

$240

Human

None

Polyclonal

Goat IgG

AF3865

100 µg

$355

Mouse/Rat

None

619

Mouse IgG1

MAB1624

100 µg

$240

Biotin

TER-119

Rat IgG2B

BAM1125

250 µg

$380

None

TER-119

Rat IgG2B

MAB1125

500 µg

$325

None

415909

Mouse IgG1

MAB4407

100 µg

$240

Tenascin R in Human Brainstem. Tenascin R was detected in paraffin-embedded human brainstem (medulla) tissue sections using anti-human Tenascin R affinity-purified polyclonal antibody (Catalog # AF3865). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

TER-119 TFF3

Mouse Human

TFF3 in Human Colon. Trefoil factor 3 (TFF3) was detected in a paraffin-embedded human colon section using anti-human TFF3 monoclonal antibody (Catalog # MAB4407). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).

TFPI-2 TGF-a

Human Human

LAP TGF-b1

Human

None

243220

Mouse IgG2A

MAB2545

500 µg

$325

None

Polyclonal

Total Goat IgG

AB-239-NA

1 mg

$355

None

Polyclonal

Goat IgG

AF-239-NA

100 µg

$355

None

Polyclonal

Total Goat IgG

AB-246-NA

1 mg

$355

None

Polyclonal

Goat IgG

AF-246-NA

100 µg

$355

TGF-b1

Multi-species

None

9016

Mouse IgG1

MAB240

500 µg

$325

TGF-b1, 2, 3

Multi-species

None

1D11

Mouse IgG1

MAB1835

500 µg

$325

TGF-b2

Multi-species

None

8607

Mouse IgG2B

MAB612

500 µg

$325

TGF-b2/1.2

Multi-species

TGF-b3

100

Multi-species

None

Polyclonal

Goat IgG

AF-302-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF-243-NA

100 µg

$355

None

20724

Mouse IgG1

MAB243

500 µg

$325

None

44922

Mouse IgG1

MAB643

500 µg

$325



LABEL

CLONE

HOST/TYPE

CATALOG #

TGF-b RI/ALK-5

MOLECULE

Human

None

Polyclonal

Goat IgG

AF3025

100 µg

SIZE

PRICE $355

TGF-b RII

Human

None

Polyclonal

Goat IgG

AF-241-NA

250 µg

$355 $355

Thioredoxin-1

Human

None

Polyclonal

Goat IgG

AF1970

100 µg

Thioredoxin Reductase 2/TRXR2 aa 268-393

Human/Mouse

None

575322

Mouse IgG1

MAB5815

100 µg

$240

Thimet Oligopeptidase/THOP1

Human

None

Polyclonal

Sheep IgG

AF3439

100 µg

$355

Human

None

501733

Mouse IgG1

MAB3947

100 µg

$240

None

Polyclonal

Goat IgG

AF3894

100 µg

$355

Thrombomodulin/THBD/CD141

Mouse

Thrombospondin-1

Human

Tie-1

Human

Phospho-Tie-2 (Y1100)

Human/Mouse

Tie-2

None

461714

Rat IgG2B

MAB3894

100 µg

$240

None

301221

Mouse IgG2B

MAB3074

500 µg

$325

None

Polyclonal

Goat IgG

AF619

100 µg

$355

None

88016

Mouse IgG1

MAB619

500 µg

$325

None

Polyclonal

Rabbit IgG

AF3909

100 µg

$285

Human

None

Polyclonal

Goat IgG

AF313

100 µg

$355

Zebrafish

None

Polyclonal

Goat IgG

AF928

100 µg

$355

Tie-2 in Developing Blood Vessels of Zebrafish. Tie-2 was detected in the blood vessels of an embryonic zebrafish using anti-zebrafish Tie-2 affinity-purified polyclonal antibody (Catalog # AF928). The tissue was stained using NorthernLights-557 donkey antigoat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).

TIM-1/KIM-1/HAVCR

Human

None

219211

Mouse IgG2B

MAB1750

500 µg

$325

Rat

None

Polyclonal

Goat IgG

AF3689

100 µg

$355

63515

Mouse IgG2B

MAB970

500 µg

$325

TIM-1 in Rat Kidney. T cell Immunoglobulin and Mucin domain-containing (TIM-1), also known as Kidney Injury Molecule 1 (KIM-1), was detected in frozen sections of rat kidney using anti-rat TIM-1 affinity-purified polyclonal antibody (Catalog # AF3689). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Human TIMP-1

TIMP-2

None

Mouse

None

Polyclonal

Goat IgG

AF980

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF580

100 µg

$355

None

Polyclonal

Goat IgG

AF971

100 µg

$355

None

89025

Mouse IgG1

MAB971

500 µg

$325

Human

TIMP-2 in Human Ovarian Cancer. Tissue Inhibitor of Metalloproteinase 2 (TIMP-2) was detected in a paraffin-embedded tissue section of human ovarian cancer using anti-human TIMP-2 affinity-purified polyclonal antibody (Catalog # AF971). Tissues were stained with the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown), and counterstained with hematoxylin (blue).

TIMP-3

Human

None

183551

Mouse IgG1

MAB973

500 µg

$325

TIMP-4

Human

None

153934

Mouse IgG1

MAB974

500 µg

$325

TLR4

Human

TMEFF1/Tomoregulin-1

Mouse

None

Polyclonal

Goat IgG

AF1478

100 µg

$355

None

Polyclonal

Goat IgG

AF1995

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1995

50 µg

$380


101


SPECIES Bovine

Canine

Equine TNF-a Human

Mouse Porcine

TNF-b/TNFSF1

TNF RI/TNFRSF1A

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF2279

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF2279

50 µg

$380

None

Polyclonal

Goat IgG

AF1507

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1507

50 µg

$380

None

236812

Mouse IgG1

MAB1507

500 µg

$325

None

Polyclonal

Goat IgG

AF1814

100 µg

$355

None

Polyclonal

Goat IgG

AF-210-NA

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF210

50 µg

$380

None

28401

Mouse IgG1

MAB610

500 µg

$325

None

Polyclonal

Goat IgG

AF-410-NA

100 µg

$355

None

Polyclonal

Goat IgG

AF690

100 µg

$355

None

103302

Mouse IgG1

MAB6903

100 µg

$240 $355

Rat

None

Polyclonal

Goat IgG

AF-510-NA

100 µg

Human

None

5802

Mouse IgG1

MAB211

500 µg

$325

Mouse

Biotin

Polyclonal

Goat IgG

BAF749

50 µg

$380

Human

None

Polyclonal

Goat IgG

AF225

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF-425-PB

100 µg

$355

Polyclonal

Total Goat IgG

AB-226-PB

1 mg

$355

TNF RI in Human Peripheral Blood Lymphocytes. Tumor Necrosis Factor Receptor I (TNF RI) was detected in human peripheral blood lymphocytes using anti-human TNF RI affinity-purified polyclonal antibody (Catalog # AF225; red) and cells were counterstained green.

TNF RII/TNFRSF1B

TOR

None

Human

None

22221

Mouse IgG2A

MAB226

500 µg

$325

Mouse

None

Polyclonal

Goat IgG

AF-426-PB

100 µg

$355

Human/Mouse

None

303728

Rat IgG2A

MAB1537

100 µg

$240

TOR in HEK293 cells. Target of rapamycin (TOR) expression was detected in HEK293 human embryonic kidney cells using anti-human TOR monoclonal antibody (Catalog # MAB1537). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).

TRa/NR1A1

Human

None

H2804

Mouse IgG2A

PP-H2804-00

100 µg

$380

TR4/NR2C2

Human

None

H0107B

Mouse IgG2A

PP-H0107B-00

100 µg

$380

Biotin

TRA-1-85

Mouse IgG1

BAM3195

100 µg

$240

TRA-1-85

Human

TRADD

Human

Human TRAIL/TNFSF10 Mouse

None

TRA-1-85

Mouse IgG1

MAB3195

100 µg

$240

None

Polyclonal

Goat IgG

AF2658

100 µg

$355

None

Polyclonal

Goat IgG

AF375

100 µg

$355

None

75411

Mouse IgG1

MAB375

500 µg

$325

None

75402

Mouse IgG1

MAB687

500 µg

$325

None

Polyclonal

Goat IgG

AF1121

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1121

50 µg

$380

TRAIL R1/TNFRSF10A

Human

None

Polyclonal

Goat IgG

AF347

100 µg

$355

TRAIL R2/TNFRSF10B

Mouse

None

118929

Rat IgG2A

MAB1540

500 µg

$325

102


R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE TRAIL R3/TNFRSF10C

SPECIES Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF630

100 µg

$355

None

90905

Mouse IgG1

MAB6301

500 µg

$325

$355

TRAIL Receptor 3 in Human Spleen. Tumor Necrosis Factor-related Apoptosis-inducing Ligand Receptor 3 (TRAIL R3) was detected in paraffin-embedded human spleen tissue sections using anti-human TRAIL Receptor 3 monoclonal antibody (Catalog # MAB630). Tissues were subjected to antigen retrieval using basic reagent (Catalog # CTS013) and stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissues were counterstained with hematoxylin (blue).

TRAIL R4/TNFRSF10D

Human

None

Polyclonal

Goat IgG

AF633

100 µg

TRANCE/TNFSF11/RANK L

Human

None

70525

Mouse IgG2B

MAB626

500 µg

$325

TRANCE/TNFSF11/RANK L

Mouse

None

Polyclonal

Goat IgG

AF462

100 µg

$355

TfR (Transferrin R)

Human

TREM-1

Mouse

Human TREM-2 Mouse Human TrkA

Rat Human

TrkB

Mouse

Human TrkC Mouse

None

Polyclonal

Goat IgG

AF2474

100 µg

$355

None

Polyclonal

Goat IgG

AF1187

100 µg

$355

Biotin

174031

Rat IgG2A

BAM1187

100 µg

$380

None

174031

Rat IgG2A

MAB1187

500 µg

$325

None

Polyclonal

Goat IgG

AF1828

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1828

50 µg

$380

None

Polyclonal

Sheep IgG

AF1729

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF1729

50 µg

$380

None

Polyclonal

Goat IgG

AF175

100 µg

$355

None

Polyclonal

Goat IgG

AF1056

100 µg

$355 $380

Biotin

Polyclonal

Goat IgG

BAF1056

50 µg

None

75133

Mouse IgG2B

MAB397

500 µg

$325

None

Polyclonal

Goat IgG

AF1494

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1494

50 µg

$380

None

225105

Rat IgG2B

MAB1494

500 µg

$325

None

Polyclonal

Goat IgG

AF373

100 µg

$355

None

75219

Mouse IgG2B

MAB373

500 µg

$325

None

Polyclonal

Goat IgG

AF1404

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1404

50 µg

$380

TrkC in Rat Spinal Cord. TrkC was detected in a cryostat tissue section of rat spinal cord using antihuman TrkC monoclonal antibody (Catalog # MAB373; red).


103


SPECIES Human

TROP-2 Mouse

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF650

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF650

50 µg

$380

None

Polyclonal

Goat IgG

AF1122

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1122

50 µg

$380

None

200805

Mouse IgG2A

MAB1874

100 µg

$240

None

Polyclonal

Goat IgG

AF723

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF723

50 µg

$380

Troponin T (Cardiac)

Human

TROY/TNFRSF19

Mouse

Trypsin Pan Specific (PRSS1/2/3)

Human

None

Polyclonal

Sheep IgG

AF3586

100 µg

$355

Trypsin 1/PRSS1

Human

None

Polyclonal

Sheep IgG

AF3848

100 µg

$355

Trypsin 3/PRSS3

Mouse

None

Polyclonal

Goat IgG

AF3565

100 µg

$355

None

419911

Rat IgG1

MAB3565

100 µg

$240

Trypsin 3 in Mouse Pancreas. Trypsin 3 was detected in a frozen section of mouse pancreas using antimouse Trypsin-3 monoclonal antibody (Catalog # MAB3565). Tissues were stained using anti-rat HRPDAB reagents (brown) and counterstained with hematoxylin (blue).

Trypsin-3 in Mouse Kidney. Trypsin-3 was detected in frozen mouse kidney sections using anti-mouse Trypsin-3 monoclonal antibody (Catalog # MAB3565). Tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).

Tryptophan Hydroxylase Pan Specific

Multi-species

None

Polyclonal

Sheep IgG

PPS038

100 µL

$355

Tryptophan Hydroxylase 1

Human

None

Polyclonal

Goat IgG

AF5276

100 µg

$355

TSG

Mouse

TSG

Mouse Human

TSG-6

Mouse

None

Polyclonal

Goat IgG

AF756

100 µg

$355

Biotin

120024

Rat IgG2A

BAM756

250 µg

$380

None

120024

Rat IgG2A

MAB756

500 µg

$325

None

Polyclonal

Goat IgG

AF2104

100 µg

$355

None

Polyclonal

Goat IgG

AF2326

100 µg

$355 $380

Biotin

Polyclonal

Goat IgG

BAF2326

50 µg

TSH b

Human

None

512913

Mouse IgG1

MAB5794

100 µg

$240

TSLP R

Human

None

Polyclonal

Goat IgG

AF981

100 µg

$355

TSPAN8

Human

None

458811

Rat IgG2B

MAB4734

100 µg

$240

Biotin

TuJ-1

Mouse IgG2A

BAM1195

100 µg

$380

None

TuJ-1

Mouse IgG2A

MAB1195

100 µg

$240

Neuron-specific b-III Tubulin

Multi-species

b-III Tubulin and Nestin in Rat Cortical Stem Cells. b-III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using anti-neuron specific b-III Tubulin monoclonal (clone TuJ-1) antibody (Catalog # MAB1195; red) and anti rat Nestin affinitypurified polyclonal antibody (Catalog # AF2736; green). Cells were stained with NorthernLights-557 donkey anti-mouse IgG (Catalog # NL007) and NorthernLights-493 donkey anti-goat IgG (Catalog # NL003) secondary antibodies. Tissue was counterstained with DAPI (blue).

Neuron-specific b-III Tubulin NorthernLights-conjugated

Multi-species

TWEAK/TNFSF12

Human

TWEAK R/TNFRSF12

Phospho-Phospho-Tyrosine

104

b-III Tubulin and GFAP in Adult Mouse Brain. Neuronal b-III Tubulin and astrocytic GFAP were detected in adult mouse brain sections using anti-b-III Tubulin monoclonal antibody (Catalog # MAB1195; Tuj1) and anti-human GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The tissue was stained using NorthernLights-493 donkey anti-mouse (Catalog # NL009; green) and NorthernLights-557 donkey anti-sheep IgG (Catalog # NL010; red) secondary antibodies, respectively. The nuclei were stained using DAPI (blue)

NL557

TuJ-1

Mouse IgG2A

NL1195R

500 µL

NL637

TuJ-1

Mouse IgG2A

NL1195V

500 µL

$345 $345

Biotin

Polyclonal

Goat IgG

BAF1090

50 µg

$380

Human

None

Polyclonal

Goat IgG

AF1199

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF1610

100 µg

$355

None

179003

Mouse IgG1

MAB1676

500 µg

$325

None

216954

Rat IgG2A

MAB16761

500 µg

$325

Multi-species



LABEL

CLONE

HOST/TYPE

CATALOG #

Phospho-Tyrosine Hydroxylase (S19)

MOLECULE

Rat

None

Polyclonal

Rabbit IgG

PPS087

100 µL

SIZE

PRICE $355


Mouse/Rat/Primate

None

Polyclonal

Rabbit IgG

PPS088

100 µL

$355


Rat

None

Polyclonal

Rabbit IgG

PPS041

100 µL

$355

None

TH-2

Mouse IgG1

MAB1423

100 µg

$240

None

6D7

Mouse IgG1

PPS040

100 µL

$355 $240

Tyrosine Hydroxylase

Multi-species

Ubiquitin/Ubiquitin+1

Human

None

83406

Mouse IgG2B

MAB701

100 µg

Ubiquitin+1

Human

None

82001

Mouse IgG2A

MAB703

100 µg

$240

ULBP-2

Human

None

Polyclonal

Goat IgG

AF1298

100 µg

$355

UNC5H1

Rat

None

Polyclonal

Goat IgG

AF1405

100 µg

$355

UNC5H2

Rat

None

Polyclonal

Goat IgG

AF1006

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1006

50 µg

$380

None

Polyclonal

Goat IgG

AF1005

100 µg

$355

UNC5H3

Human

None

200414

Mouse IgG2B

MAB1005

500 µg

$325

u-Plasminogen Activator (uPA)/Urokinase

Human

None

Polyclonal

Goat IgG

AF1310

100 µg

$355

uPAR

Mouse

None

Polyclonal

Goat IgG

AF534

100 µg

$355

Human

None

Polyclonal

Sheep IgG

AF5144

100 µg

$355

Uromodulin

Uromodulin in Human Kidney. Uromodulin was detected in paraffin-embedded human kidney sections using anti-human Uromodulin affinity-purified polyclonal antibody (Catalog # AF5144). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).

Uteroglobin/SCGB1A1

Human

None

394324

Rat IgG1

MAB4218

100 µg

$240

UTF1

Human

None

Polyclonal

Goat IgG

AF3958

100 µg

$355

Human/Mouse

None

Polyclonal

Goat IgG

AF4828

100 µg

$355

VAMP-1

VAMP-1 in Mouse Brain. Vesicle-associated membrane protein 1 (VAMP-1) was detected in frozen sections of mouse brain (spinal cord) using anti-human/ mouse VAMP-1 affinity-purified polyclonal antibody (Catalog # AF4828). The tissue was stained using antigoat IgG NorthernLights-557-conjugated secondary antibody (Catalog # NL001; red) and counterstained green.

Vang-like Protein 1/VANGL1

Human

None

497707

Mouse IgG2B

MAB5476

100 µg

$240

Vanilloid R1/TRPV1

Rat

None

Polyclonal

Goat IgG

AF3066

100 µg

$355

Vanilloid R1 in Rat Dorsal Root Ganglion. Vanilloid R1 was detected in a frozen section of rat dorsal root ganglion using anti-rat Vanilloid R1 affinity-purified polyclonal antibody (Catalog # AF3066; red) and counterstained green.

Vanilloid R-like 3/TRPV3

Human

None

Polyclonal

Sheep IgG

AF4167

100 µg

$355

VASA

Human

None

Polyclonal

Goat IgG

AF2030

100 µg

$355


105


VCAM-1/CD106

VDR/NR1I1 VEGF

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat Serum

BBA19

500 µL

$195

Human

None

BBIG-V1

Mouse IgG1

BBA5

100 µg

$230

None

BBIG-V1

Mouse IgG1

BBA6

1 mg

$840

Mouse

None

Polyclonal

Goat IgG

AF643

100 µg

$355

Human

None

H4537

Mouse IgG2A

PP-H4537-00

100 µg

$380 $325

Canine

None

247109

Mouse IgG1

MAB1603

500 µg

Human

None

26503

Mouse IgG2B

MAB293

500 µg

$325

None

Polyclonal

Goat IgG

AF1603

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF1603

50 µg

$380

Canine VEGF 164

Mouse

None

Polyclonal

Goat IgG

AF-493-NA

100 µg

$355

Rat

None

Polyclonal

Goat IgG

AF564

100 µg

$355

VEGF in Mouse Kidney. Vascular Endothelial Growth Factor (VEGF) was detected in a cryostat section of mouse kidney glomerulus using anti-mouse VEGF affinity-purified polyclonal antibody (Catalog # AF493-NA; red) and counterstained green.

VEGF in Rat Kidney. Vascular Endothelial Growth Factor (VEGF) was detected in a cryostat section of rat kidney glomerulus using anti-rat VEGF 164 affinitypurified polyclonal antibody (Catalog # AF564). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

None

Polyclonal

Total Goat IgG

AB-293-NA

1 mg

$355

None

Polyclonal

Goat IgG

AF-293-NA

100 µg

$355

Human

None

56-1

Mouse IgG1

MAB3045

500 µg

$325

Human/Mouse

None

58013

Mouse IgG1

MAB751

500 µg

$325

VEGF 165

Human

VEGF 165b VEGF-B 167/186 VEGF-B 186

Mouse

None

Polyclonal

Goat IgG

AF767

100 µg

$355

VEGF-C

Human

None

Polyclonal

Goat IgG

AF752

100 µg

$355

None

Polyclonal

Goat IgG

AF286

100 µg

$355

None

78923

Mouse IgG1

MAB286

500 µg

$325

None

Polyclonal

Goat IgG

AF469

100 µg

$355

None

Polyclonal

Goat IgG

AF321

100 µg

$355 $380

VEGF-D

Human Mouse

VEGF R1/Flt-1

Human Mouse Human

VEGF R2/KDR/Flk-1 Mouse

Biotin

Polyclonal

Goat IgG

BAF321

50 µg

None

141515

Rat IgG2A

MAB471

500 µg

$325

None

Polyclonal

Goat IgG

AF357

100 µg

$355

None

89115

Mouse IgG1

MAB3571

500 µg

$325

None

Polyclonal

Goat IgG

AF644

100 µg

$355

None

522302

Rat IgG1

MAB4432

100 µg

$240

None

Polyclonal

Goat IgG

AF349

100 µg

$355

None

54703

Mouse IgG1

MAB3491

500 µg

$325

VEGF R3/Flt-4

Human

Versican Isoform V0

Human

None

Polyclonal

Goat IgG

AF3054

100 µg

$355

VG5Q

Mouse

None

368011

Rat IgG2A

MAB30481

100 µg

$240

VHR

Human

None

237018

Mouse IgG1

MAB16541

100 µg

$240

None

Polyclonal

Goat IgG

AF2105

100 µg

$355

None

280618

Rat IgG2A

MAB2105

100 µg

$240

Vimentin

Human

Vimentin in Human Lung Carcinoma Cells. Vimentin was detected in cultured A549 human lung carcinoma cells cells using anti-human Vimentin monoclonal antibody (Catalog # MAB2105). The Cells were stained using NorthernLights-493 anti-rat secondary antibodies (Catalog # NL015; green) and the nuclei counterstained with DAPI (blue).

106


IHC/ICC SUPPLEMENTAL REAGENTS MOLECULE VIP R2 Vitronectin VLDL R VSIG1

SPECIES

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

Human

None

476031

Mouse IgG1

MAB5416

100 µg

$240

Human

None

342603

Mouse IgG2B

MAB2349

100 µg

$240

Mouse

None

347317

Rat IgG2A

MAB38751

100 µg

$240

Mouse

None

Polyclonal

Goat IgG

AF2258

100 µg

$355

Human

None

Polyclonal

Sheep IgG

AF4818

100 µg

$355

VSIG1 in Human Testis. V-set and Ig domain-containing protein 1 (VSIG1) was detected in frozen a section of human testis using anti-human VSIG1 affinity-purified polyclonal antibody (Catalog # AF4818). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).

WASP

WIF-1

Human

None

336925

Mouse IgG1

MAB3070

100 µg

$240

Human/Mouse

None

Polyclonal

Goat IgG

AF3070

100 µg

$355

None

133015

Mouse IgG1

MAB134-050

50 µg

$150

Human Human

WISP-1/CCN4

Wnt-1 Wnt-2

Mouse

None

133015

Mouse IgG1

MAB134-500

500 µg

$325

None

Polyclonal

Goat IgG

AF1627

100 µg

$355

None

Polyclonal

Sheep IgG

AF1680

100 µg

$355

Biotin

Polyclonal

Sheep IgG

BAF1680

50 µg

$380

Mouse

None

Polyclonal

Goat IgG

AF1620

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF3464

100 µg

$355

Wnt-2 in Human Stomach Cancer. Wnt-2 was detected in a paraffin-embedded human stomach cancer section using anti-human Wnt-2 affinity-purified polyclonal antibody (Catalog # AF3464). The section was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). The tissue was counterstained with hematoxylin (blue).

Wnt-2b

Mouse

None

Polyclonal

Goat IgG

AF3900

100 µg

$355

Wnt-4

Mouse

None

Polyclonal

Goat IgG

AF475

100 µg

$355

Mouse

None

Polyclonal

Goat IgG

AF645

100 µg

$355

Wnt-5a

Wnt-5a in Embryonic Mouse Rib. Wnt-5a was detected in a paraffin-embedded tissue section of E15 mouse rib using anti-mouse Wnt-5a affinity-purified polyclonal antibody (Catalog # AF645). The tissue was stained using the anti-goat HRP-DAB (brown) Cell and Tissue Staining Kit (Catalog # CTS008) and counterstained with hematoxylin (blue).

Wnt-5b

Mouse

None

325004

Rat IgG2A

MAB3006

100 µg

$240

Wnt-6

Human

None

Polyclonal

Sheep IgG

AF4109

100 µg

$355

Wnt-7a

Human

None

Polyclonal

Goat IgG

AF3008

100 µg

$355

Wnt-7b

Human

None

Polyclonal

Goat IgG

AF3460

100 µg

$355

Wnt-8a

Mouse

None

Polyclonal

Goat IgG

AF2248

100 µg

$355

Wnt-8b

Human/Mouse

None

341504

Rat IgG2A

MAB3367

100 µg

$240


107

IHC/ICC SUPPLEMENTAL REAGENTS MOLECULE Wnt-9a

SPECIES Human

LABEL

CLONE

HOST/TYPE

CATALOG #

SIZE

PRICE

None

Polyclonal

Goat IgG

AF3157

100 µg

$355

None

326707

Mouse IgG2A

MAB31571

100 µg

$240

Wnt-9a in Human Breast Cancer. Wnt-9a was detected in a paraffin-embedded human breast cancer section using anti-human Wnt-9a monoclonal antibody (Catalog # MAB31571). The section was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). The tissue was counterstained with hematoxylin (blue).

Wnt-9b

Mouse

None

Polyclonal

Goat IgG

AF3669

100 µg

$355

Wnt-11

Mouse

None

Polyclonal

Goat IgG

AF2647

100 µg

$355

XCL1/Lymphotactin

Mouse

None

Polyclonal

Goat IgG

AF486

100 µg

$355

Biotin

Polyclonal

Goat IgG

BAF486

50 µg

$380

XCR1

Human

None

Polyclonal

Goat IgG

AF857

100 µg

$355

Human

None

117318

Mouse IgG1

MAB8221

100 µg

$240 $355

XIAP

Yes aa 1-88 ZAP70 aa 163-254

Human/Mouse/Rat

None

Polyclonal

Goat IgG

AF8221

100 µg

Human/Mouse/Rat

None

339827

Mouse IgG2B

MAB32051

100 µg

$240

Human/Rat

None

Polyclonal

Goat IgG

AF3205

100 µg

$355

Human

None

Polyclonal

Goat IgG

AF3709

100 µg

$355

Polyclonal

Goat IgG

AF4978

100 µg

$355

ZAP70 in Human Lymphoma. Zeta-chain (TCR) associated protein kinase 70 (ZAP70) was detected in a paraffin-embedded human lymphoma section using anti-human ZAP70 affinity-purified polyclonal antibody (Catalog # AF3709). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). The section was counterstained with hematoxylin (blue).

ZIC1

Human/Mouse

None

ZIC1 in Embryonic Mouse Brain. Zinc-finger protein of the cerebellum 1 (ZIC1) was detected in a frozen section of E13 mouse brain using anti-human ZIC1 affinity-purified polyclonal antibody (Catalog # AF4978). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

ZNF24

Human

None

Polyclonal

Goat IgG

AF3637

100 µg

$355

ZNF206

Human

None

Polyclonal

Goat IgG

AF4020

100 µg

$355

108


IHC/ICC SUPPLEMENTAL REAGENTS Secondary & Control Antibodies for IHC/ICC SECONDARY ANTIBODIES analyte Chicken IgY

Goat IgG

Goat IgG (H+L)

Hamster IgG

type

applications

catalog #

size

price

Chicken IgY specific polyclonal goat IgG

Western blot

AF010

500 µg

$75

Biotin

Chicken IgY specific polyclonal goat IgG

Western blot

BAF010

250 µg

$75

None

Goat IgG specific polyclonal donkey IgG

Western blot

AF109

500 µg

$75

Biotin


Western blot

BAF109

250 µg

$75

Biotin

Goat IgG specific polyclonal rabbit IgG

Western blot

BAF017

250 µg

$75

Biotin

Goat IgG specific polyclonal chicken IgY

Western blot

BAF019

250 µg

$75

HRP


Western blot

HAF109

1 mL

$95

HRP


Western blot

HAF017

1 mL

$95

HRP

Goat IgG specific polyclonal chicken IgG

Western blot

HAF019

1 mL

$95

APC


Flow Cytometry

F0108

100 Tests

$220

Fluorescein


Flow Cytometry

F0109

100 Tests

$140

PE


Flow Cytometry

F0107

100 Tests

$175

None


Western blot

R-401-C-ABS

PerCP

Goat IgG specific polyclonal donkey IgG (H+L) chains

Flow Cytometry

APC

Monoclonal mouse IgG2B, clone # MAH1.12

PE


None Biotin

500 µg

$75

F0124

100 Tests

$190

Flow Cytometry

F0121

100 Tests

$220

Flow Cytometry

F0120

100 Tests

$175


Western blot

MAB011

500 µg

$165


Western blot

BAM011

250 µg

$180

Human IgG Fc

None

Human IgG Fc specific polyclonal goat IgG

Western blot

G-102-C

100 µg

$355

Human IgG1 Fc

None

Human IgG1 Fc specific monoclonal mouse IgG1

Western blot

MAB110

500 µg

$325

Human IgM

None

Human IgM specific polyclonal goat IgG

Western blot

G-105-C

100 µg

$355

Human IgE

None

Human IgE specific polyclonal goat IgG

Western blot

G-107-C

100 µg

$355

Human IgG (H+L)

None

Human IgG specific polyclonal goat IgG (H+L) chains

Western blot

G-101-C-ABS

500 µg

$75

PerCP

Mouse IgG specific polyclonal goat IgG F(ab')2 (H+L) Fragment

Flow Cytometry

F0114

100 Tests

$155

None

Mouse IgG specific polyclonal goat IgG

Western blot

AF007

500 µg

$99

Biotin


Western blot

BAF007

250 µg

$100

Biotin

Mouse IgG specific polyclonal donkey IgG

Western blot

BAF018

250 µg

$75

HRP


Western blot

HAF007

1 mL

$105

HRP


Western blot

HAF018

1 mL

$95

Mouse IgG Fc

None

Mouse IgG Fc specific polyclonal goat IgG

Western blot

G-202-C

100 µg

$355

Mouse IgG

None


Western blot

D-201-C-ABS2

500 µg

$75

APC

Mouse IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment

Flow Cytometry

F0101B

100 Tests

$175

Fluorescein


Flow Cytometry

F0103B

100 Tests

$105

PE


Flow Cytometry

F0102B

100 Tests

$135

None

Rabbit IgG specific polyclonal goat IgG

Western blot

AF008

500 µg

$75

Biotin


Western blot

BAF008

250 µg

$75

HRP


Western blot

HAF008

1 mL

$95

Mouse IgG

Mouse IgG F(ab’)2 (H+L)

Rabbit IgG

Rat IgG

Rat IgG F(ab’)2 Rat IgG F(ab’)2 (H+L)

label None

APC


Flow Cytometry

F0111

100 Tests

$220

Fluorescein


Flow Cytometry

F0112

100 Tests

$140

PE


Flow Cytometry

F0110

100 Tests

$175

None

Rabbit IgG specific polyclonal donkey IgG

Western blot

D-301-C-ABS2

HRP

Rat specific polyclonal goat IgG

Western blot

HAF005

None

Rat IgG specific polyclonal goat IgG

Western blot

PerCP

Rat IgG specific polyclonal goat IgG F(ab')2 (H+L) Fragment

Biotin

Rat IgG specific polyclonal goat IgG

APC

500 µg

$75

1 mL

$105

AF005

500 µg

$100

Flow Cytometry

F0115

100 Tests

$190

Western blot

BAF005

250 µg

$100

Rat IgG specific polyclonal goat IgGF(ab’)2 (H+L) Fragment

Flow Cytometry

F0113

100 Tests

$210

Fluorescein

Rat IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment

Flow Cytometry

F0104B

100 Tests

$120

PE

Rat IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment

Flow Cytometry

F0105B

100 Tests

$175


109

IHC/ICC SUPPLEMENTAL REAGENTS SECONDARY ANTIBODIES analyte

Sheep IgG

Mouse IgM

label

type

applications

catalog #

size

price

Biotin

Sheep IgG specific polyclonal donkey IgG

Western blot

BAF016

250 µg

$75

HRP


Western blot

HAF016

1 mL

$95

None


Western blot

D-501-C-ABS

500 µg

$75

APC

Mouse IgM specific polyclonal goat IgG

Flow Cytometry

F0117

100 Tests

$220

Fluorescein


Flow Cytometry

F0118

100 Tests

$140

PerCP


Flow Cytometry

F0119

100 Tests

$190

PE


Flow Cytometry

F0116

100 Tests

$175

NorthernLights™ A line of fluorochrome-conjugated secondary reagents for IHC/ICC NorthernLights secondary antibodies are bright and fade-resistant, recognizing mouse, goat, sheep, rat, and rabbit IgG and chicken IgY. Like all R&D Systems antibodies, NorthernLights antibody conjugates are specific and deliver a high signal-to-noise ratio. Also available are streptavidin conjugates for labeling biotinylated antibodies. description

conjugate

catalog #

NorthernLights-557 Donkey Anti-Goat IgG, Cross-adsorbed

NorthernLights-637

Donkey Anti-Rabbit IgG, Cross-adjusted

Donkey Anti-Mouse IgG, Cross-adsorbed

size

price

NL001

500 µL

$115

NL002

500 µL

$115

NorthernLights-493

NL003

500 µL

$115

NorthernLights-557

NL004

500 µL

$115

NorthernLights-637

NL005

500 µL

$115

NorthernLights-493

NL006

500 µL

$115

NorthernLights-557

NL007

500 µL

$115

NorthernLights-637

NL008

500 µL

$115

NorthernLights-493

NL009

500 µL

$115

NorthernLights-557

NL010

500 µL

$115

NorthernLights-637

NL011

500 µL

$115

NorthernLights-493

NL012

500 µL

$115

NorthernLights-557

NL013

500 µL

$115

NorthernLights-637

NL014

500 µL

$115

NorthernLights-493

NL015

500 µL

$115

NorthernLights-557

NL016

500 µL

$115

NorthernLights-637

NL017

500 µL

$115

NorthernLights-493

NL018

500 µL

$115

Streptavidin NL493

NorthernLights-493

NL997

500 µL

$115

Streptavidin NL637

NorthernLights-637

NL998

500 µL

$115

Streptavidin NL557

NorthernLights-557

NL999

500 µL

$115

Donkey Anti-Sheep IgG, Cross-adsorbed

Goat Anti-Rat IgG

Goat Anti-Chicken IgY

ANTIBODY CONTROLS control

Goat IgG

label

type

catalog #

size

price

Fluorescein

Normal goat IgG

IC108F

200 Tests

$165

PE

Normal goat IgG

IC108P

200 Tests

$165

PerCP

Normal goat IgG

IC108C

200 Tests

$165

None

Normal goat IgG

AB-108-C

1 mg

$75

Biotin

Normal goat IgG

BAF108

100 µg

$75

Human IgG1 Fc

None

NS0-expressed recombinant protein

110-HG-100

100 µg

$325

Human IgG

None

Normal human IgG

1-001-A

1 mg

$75

None

Normal sheep IgG

5-001-A

1 mg

$75

APC

Normal sheep IgG

IC016A

200 Tests

$165

Fluorescein

Normal sheep IgG

IC016F

200 Tests

$165

Sheep IgG

110


IHC PROTOCOL GUIDE ANTIBODY CONTROLS, continued control

Mouse IgG1

Mouse IgG2A

Mouse IgG2A Fc

Mouse IgG2B

Mouse IgG3

Rabbit IgG

Rat IgG

Rat IgG1

Rat IgG2A

Rat IgG2B

label

type

catalog #

size

price

PerCP

Normal mouse IgG1, clone # 11711

IC002C

200 Tests

$165

APC


IC002A

200 Tests

$165

None


MAB002

500 µg

$170

Biotin


IC002B

200 Tests

$165

Fluorescein


IC002F

200 Tests

$165

PE


IC002P

200 Tests

$165

None

Normal mouse IgG2B, clone # 20102

MAB003

500 µg

$170

None


MAB0031

500 µg

$170

PerCP


IC003C

200 Tests

$165

APC


IC003A

200 Tests

$165

Biotin


IC003B

200 Tests

$165

Fluorescein


IC003F

200 Tests

$165

PE


IC003P

200 Tests

$165

None

NS0-expressed recombinant protein

4460-MG-100

100 µg

$325 $170

None


MAB004

500 µg

None


MAB0041

500 µg

$170

None

Normal mouse IgG2BB, clone # 73009

MAB0042

500 µg

$170

APC


IC0041A

200 Tests

$165

Fluorescein


IC0041F

200 Tests

$165

PE


IC0041P

200 Tests

$165

PerCP


IC0041C

200 Tests

$165

None


MAB007

500 µg

$170

APC

Normal Mouse IgG3, clone # 133316

IC007A

200 Tests

$165

Fluorescein


IC007F

200 Tests

$165

PE


IC007P

200 Tests

$165

APC

Normal rabbit IgG

IC105A

200 Tests

$165

Fluorescein

Normal rabbit IgG

IC105F

200 Tests

$165

PE

Normal rabbit IgG

IC105P

200 Tests

$165

None

Normal rabbit IgG

AB-105-C

1 mg

$75

None

Normal rat IgG

6-001-A

1 mg

$75

None

Normal rat IgG1, clone # 43414

MAB005

500 µg

$170

APC


IC005A

200 Tests

$165

Biotin


IC005B

200 Tests

$165

Fluorescein


IC005F

200 Tests

$160

PE


IC005P

200 Tests

$165

None

Normal rat IgG2A, clone # 54447

MAB006

500 µg

$170

APC


IC006A

50 µg

$165

Biotin


IC006B

50 µg

$165

Fluorescein


IC006F

200 Tests

$165

PE


IC006P

200 Tests

$165

PerCP

Normal rat IgG, clone # 54447

IC006C

200 Tests

$165

PerCP

Normal rat IgG, clone # 141945

IC013C

200 Tests

$165

APC

Normal rat IgG2B, clone # 141945

IC013A

200 Tests

$165

Biotin


IC013B

200 Tests

$165

Fluorescein


IC013F

200 Tests

$165

PE


IC013P

200 Tests

$165

None


MAB0061

500 µg

$170

Mouse IgG3

PerCP

Normal mouse IgG, clone # 133316

IC007C

200 Tests

$165

Mouse IgM

PerCP

Normal mouse IgM

IC015C

200 Tests

$165

Chicken IgY

None

Normal chicken IgY

AB-101-C

1 mg

$75


111

IHC PROTOCOL GUIDE Staining Kits & Reagents for Cells & Tissues Cell and Tissue Staining Kits are intended for localization of antigens in a broad range of histological and cytological specimens. These kits are based on the formation of the Avidin-Biotin Complex (ABC) with primary antibodies that react with the tissue antigens under study. The kits are made in ready-to-use format including prediluted Secondary Biotinylated Antibodies, and HSS-HRP that eliminates the need for extra steps in the procedure, minimizes hands-on time, maximizes convenience, and reduces the risk of erroneous calculations. Cell & Tissue Staining Kits species

label

components

catalog #

Size

Price

Anti-goat Anti-mouse Anti-rabbit Anti-rat Anti-sheep

HRP-DAB System

Secondary Biotinylated Antibody, Streptavidin-HRP Conjugate, DAB Chromogen, DAB Chromogen Buffer, Blocking Reagents

CTS008 CTS002 CTS005 CTS017 CTS019

50 Tests

$240

Anti-goat Anti-mouse Anti-rabbit Anti-rat Anti-sheep

HRP-AEC System

Secondary Biotinylated Antibody, Streptavidin-HRP Conjugate, AEC Chromogen, AEC Chromogen Buffer, Blocking Reagents

CTS009 CTS003 CTS006 CTS018 CTS020

50 Tests

$240

product

description

catalog #

Size

Price

p27/Kip1 IHC Detection Kit

Includes reagents for antigen retrieval, blocking reagent, detection reagents, and a specific monoclonal primary antibody for human p27 antigen

CTS2256

50 Tests

$425

Size

Price

p27 in Human Prostate Cancer. p27 was detected in paraffin-embedded human prostate cancer sections using the Human p27 Immunohistochemistry Detection Kit (Catalog # CTS2256). Tissue was stained using anti-mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).

Cell & Tissue Staining Reagents product

description

catalog #

Basic Antigen Retrieval Reagent

Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens.

CTS013

50 mL

$60

Acidic Antigen Retrieval Reagent


CTS014

50 mL

$60

Universal Antigen Retrieval Reagent


CTS015

50 mL

$60

Antigen Retrieval Reagent Sampler

All three reagents (CTS013, CTS014, CTS015) in one convenient pack.

CTS016

50 mL

$120

Avidin-Fluorescein

For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated primary labeling reagents.

F0030

100 Tests

$65

Streptavidin-Allophycocyanin


F0050

100 Tests

$85

Streptavidin-Phycoerythrin


F0040

100 Tests

$75

Streptavidin-Alkaline Phosphatase

For use in ELISA or Western blot assays.

AR001

1 mL

$145

DAB Enhancer

DAB Enhancer is a concentrated 50X metal based solution used to intensify the DABChromogen (Catalog Numbers # CTS002, CTS005, CTS008, CTS017). In the presence of the DAB Enhancer, the DAB Chromogen produces a clear brown-black staining with minimal background.

CTS010

3 mL

$45

Mounting Medium

Permanent aqueous mounting medium

CTS011

15 mL

$35

Control

112

Antigen Retrieval

Antigen Retrieval Improves Detection of Neuroplastin 65. Neuroplastin 65 was detected in human cerebellar tissue using anti-human/mouse Neuroplastin 65 affinity-purified polyclonal antibody (Catalog # AF5360). Tissue was pretreated with Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the anti-Goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).


Ordering Information Customers IN Usa & CANADA Online ordering is available for customers in the U.S.A. and Canada. After finding your products, click the shopping cart symbol to create an account or to make an online purchase. Website: www.RnDSystems.com Phone: 1-800-343-7475 Fax:

1-612-656-4400

Mail:

R&D Systems, Inc. 614 McKinley Place NE Minneapolis, MN 55413

International Distributors Please visit www.RnDSystems.com for a full list of international distributors.

For all purchases, please include the following information with your order • Name & Contact Information • Name of Institution • Delivery Address • Invoice Address • Purchase Order Number* or Credit Card Number • Quantity and Product Size • Product Catalog Number and Description • Name of Principal Investigator (optional) • Telephone Number • E-mail Address (optional) For European Customers: VAT Exemption Certificate (UK) or E.C. equivalent * New customers wishing to use a P.O. number, please call customer service at 1-800-343-7475 to set up an account.

New IHC/ICC Screensavers!

To download R&D Systems IHC/ICC image screensavers, please visit our website at: www.RnDSystems.com/go/Screensavers

USA & Canada R&D Systems, Inc. 614 McKinley Place NE Minneapolis, MN 55413, USA TEL: (800) 343-7475 (612) 379-2956 FAX: (612) 656-4400 E-MAIL: [email protected] www.RnDSystems.com

Europe R&D Systems Europe Ltd. 19 Barton Lane, Abingdon Science Park Abingdon OX14 3NB, UK TEL: +44 (0)1235 529449 FAX: +44 (0)1235 533420 E-MAIL: [email protected] www. RnDSystems.com

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International Distributors Please visit www.RnDSystems.com/go/Distributors for a full list of international distributors. CA103_IHCprotocol_MAR