Dec 16, 2016 - that the recombinant Bacillus subtilis B.S.-HA could be sampled by ..... R. Genetic system constructed to overproduce and secrete proinsulin in ...
www.nature.com/scientificreports
OPEN
received: 21 June 2016 accepted: 17 October 2016 Published: 16 December 2016
Immune Responses Induced by Recombinant Bacillus Subtilis Expressing the Hemagglutinin Protein of H5N1 in chickens Chunxiao Mou, Liqi Zhu, Jingjing Yang, Wenwen Xu, Xiaoying Cheng & Qian Yang To develop an effective, safe, and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI) H5N1, we have constructed a recombinant Bacillus subtilis strain (B.S.-HA) expressing the hemagglutinin (HA) protein. Then we evaluated the immune function in chicken bone marrow derived dendritic cells (BM-DCs), and the immune response after oral immunization. Our results show that the recombinant Bacillus subtilis B.S.-HA could be sampled by BM-DCs in vitro and increase the BM-DCs major histocompatibility complex (MHC) II phenotype. The weight, height of the small intestine villus, and lymphoid tissue area of the ileum increased significantly in B.S.-HA immunized chickens (P
