AND HARRY W. SNYDER, JRI'3*3~. Laboratories of 1 Viral .... A-MuLV Pl20g~g -~bl was immunoprecipitated from a lysate of A-MuLV- transformed NIH 3T3 ...
J.
gen. Virol. (1985), 66, 2057-2063.
Printedin Great Britain
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Key words: FeSV/A-MuL V/tran,~'[brmingpr~tein/protein kinase
Immunological and Biochemical Characterization of HZ2 Feline Sarcoma Virus and Abelson Murine Leukaemia Virus Translation Products By L Y N N E L E D E R M A N , 1 , 3 t M I T R A C. S I N G H A L , 1 P E T E R B E S M E R , 2"3 E V E L Y N E. Z U C K E R M A N , z W I L L I A M D. H A R D Y , JR 2"3 AND H A R R Y W. S N Y D E R , JRI'3*3~ Laboratories o f 1Viral Oncology and 2 Veterinary Oncology, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021 and 3Molecular Biology and Virology Unit, Cornea University Graduate School o f Medical Sciences, Sloan-Kettering Division, 1275 York Avenue, New York, New York 10021, U.S.A. (Accepted 23 May 1985)
SUMMARY
The extent of homology between the translation products of the HZ2 strain of feline sarcoma virus (HZ2-FeSV) and the Abelson murine leukaemia virus (A-MuLV) was examined immunologically and biochemically. Antiserum prepared against the v-ablencoded determinants of the A-MuLV polyprotein P120ga~-ab~ was also found to precipitate specifically the 98K mol. wt. HZ2-FeSV protein (P98gag-~bl). The basis for this immunological crossreactivity was indicated by the findings that the two proteins had at least six [35S]methionine-containing tryptic peptides and at least eight [35S]methionine-containing chymotryptic peptides in common. Each of the two proteins also had tryptic and chymotryptic peptides which were unique. Both proteins were associated with tyrosyl kinase activities which exhibited some similar biochemical properties in vitro. However, the HZ2-FeSV-associated activity was much more sensitive to competitive inhibition by nucleoside and deoxynucleoside diphosphates than was the A-MuLV-associated activity. These results suggest that, while the gag-abl translation products of these two independent isolates of transforming retrovirus are highly related structurally and functionally, the differences in structure contribute to differences in enzyme activity. Further comparative studies of these two proteins should play an important role in determining their roles in induction of two different types of malignancy: lymphosarcoma in the case of the AMuLV protein and fibrosarcoma in the case of the HZ2-FeSV protein. HZ2 feline sarcoma virus (HZ2-FeSV) is a replication-defective acute transforming retrovirus obtained from a fibrosarcoma of a young pet cat (Hardy et al., 1982; Besmer et al., 1983). This virus contains genetic sequences homologous to those of the v-abloncogene (Besmer et al., 1983), initially described as a component of the Abelson murine leukaemia virus (A-MuLV) genome (Abelson & Rabstein, 1970a; Goffet al., 1980). Southern blot analysis of mink cell-derived HZ2FeSV DNA and normal mink, cat and mouse D N A using a ~,-32p-labelled v-abl probe (Goffet al., 1980) and both high and low stringency washing conditions indicated a likely feline origin for the v-abl sequences in HZ2-FeSV (Besmer et al., 1983). Results also indicated a high degree of relatedness between the cat- and mouse-derived viral sequences. The v-abl sequences in HZ2FeSV were shown to be linked to feline leukaemia virus (FeLV) sequences in the integrated t Present address: Department of Biochemistry, Boston University Medical School, 80 East Concord Street, Boston, Massachusetts 02118, U.S.A. :1:Present address: Immune Response Program, Pacific Northwest Research Foundation, 1102 Columbia Street, Seattle, Washington 98104, U.S.A. 0000-6480© 1985 SGM
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