... Microbiota Modulation on. High-Fructose-Fed Rats. Prasant Kumar Jena, Shilpa Singh,. Bhumika Prajapati, G. Nareshkumar,. Tejal Mehta & Sriram Seshadri ...
Impact of Targeted Specific Antibiotic Delivery for Gut Microbiota Modulation on High-Fructose-Fed Rats Prasant Kumar Jena, Shilpa Singh, Bhumika Prajapati, G. Nareshkumar, Tejal Mehta & Sriram Seshadri Applied Biochemistry and Biotechnology Part A: Enzyme Engineering and Biotechnology ISSN 0273-2289 Appl Biochem Biotechnol DOI 10.1007/s12010-014-0772-y
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Author's personal copy Appl Biochem Biotechnol DOI 10.1007/s12010-014-0772-y
Impact of Targeted Specific Antibiotic Delivery for Gut Microbiota Modulation on High-Fructose-Fed Rats Prasant Kumar Jena & Shilpa Singh & Bhumika Prajapati & G. Nareshkumar & Tejal Mehta & Sriram Seshadri
Received: 26 August 2013 / Accepted: 3 February 2014 # Springer Science+Business Media New York 2014
Abstract The objective of present investigation was to study the effect of gut microbiota alteration by oral administration of targeted delivery of pH sensitive cefdinir microspheres to high-fructose-fed (HFD) rats. Rats were fed with a high-fructose diet with or without cefdinir microsphere administration for 30 days. The fecal microbiota community, oral glucose tolerance, the markers of liver injury, plasma and hepatic lipids profile, and histological evaluation were investigated. The levels of blood glucose, liver injury markers, lipid profile in plasma and liver, and fat tissue were significantly increased in high-fructose-fed rats. However, after pH-sensitive cefdinir microsphere administration, the elevation of these parameters was significantly suppressed. Cef EL significantly lowered the increased AST (p0.05 when compared with HFD group; a p>0.05; b p>0.01; c p>0.001 when compared with CD group
rats (Fig. 6, series 3B) while reduced histological parameters of colonic injury was observed in Cef EL (Fig. 6, series 3C) as well as of control group (Fig. 6, series 3A). Enumeration of Fecal Microbiota Bifidobacteria and lactobacilli have been regarded as beneficial microflora species, whereas some species, Enterobacteriaceae for example, would be harmful as a consequence of their metabolic activities. Quantification of fecal bacteria of experimental animal was showed (Fig. 7). The number of bifidobacteria, lactobacilli, and Enterobacteriaceae in control group was regarded as 100 % for comparison. Our present results indicated that the number of Enterobacteriaceae, lactobacilli, and bifidobacteria in HFD group were 146.56±27.28,
Fig. 6 Effects of Cef EL on HFD treated rats. At the end of the experimental period, the livers, distal ileum, and proximal colon from sacrificed rats were collected. a Control. b HFD group. c HFD Cef EL (in series 1: CV central vein, KC kupffer cell; in series 2: CP crypts, LP lamina propria, SM submucosa, AD adventitia; in series 3: CE crypt epithelium, GC goblet cell, CP crypts)
Author's personal copy Appl Biochem Biotechnol
Fig. 7 In vivo quantification of fecal bacteria from experimental animal. Values are presented as mean±SEM (n=3). Values with superscript letters are significantly compared with control (*p
