In Vitro Biocompatibility of Electrospun Chitosan/Collagen Scaffold

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Aug 22, 2013 - [9] W. W. Thein-Han, Y. Kitiyanant, and R. D. K. Misra, “Chitosan as scaffold matrix for tissue engineering,” Materials Science and. Technology ...
Hindawi Publishing Corporation Journal of Nanomaterials Volume 2013, Article ID 958172, 8 pages http://dx.doi.org/10.1155/2013/958172

Research Article In Vitro Biocompatibility of Electrospun Chitosan/Collagen Scaffold Peiwei Wang,1,2 Junli Liu,3 and Teng Zhang1,2 1

Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China 2 Clinical Institute of Integrative Chinese and Western Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai 200437, China 3 Department of Textile and Chemical Engineering, Changzhou Textile Garment Institute, Changzhou 213164, China Correspondence should be addressed to Peiwei Wang; [email protected] and Teng Zhang; [email protected] Received 14 June 2013; Revised 31 July 2013; Accepted 22 August 2013 Academic Editor: Xiaoming Li Copyright © 2013 Peiwei Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Chitosan/collagen composite nanofibrous scaffold has been greatly concerned in recent years for its favorable physicochemical properties which mimic the native extracellular matrix (ECM) both morphologically and chemically. In a previous study, we had successfully fabricated nanofibrous chitosan/collagen composite by electrospinning. In the present study, we further investigate the biocompatibility of such chitosan/collagen composite nanofiber to be used as scaffolds in vascular tissue engineering. The porcine iliac artery endothelial cells (PIECs) were employed for morphogenesis, attachment, proliferation, and phenotypic studies. Four characteristic EC markers, including two types of cell adhesion molecules, one proliferation molecule (PCNA), and one function molecule (p53), were studied by semiquantitative RT-PCR. Results showed that the chitosan/collagen composite nanofibrous scaffold could enhance the attachment, spreading, and proliferation of PIECs and preserve the EC phenotype. Our work provides profound proofs for the applicable potency of scaffolds made from chitosan/collagen composite nanofiber to be used in vascular tissue engineering.

1. Introduction Vascular disease is the main cause of death in Western societies [1]. Coronary artery and peripheral vascular diseases are the leading causes of mortality, necessitating surgical interventions including small-diameter bypass grafting with autologous veins or arteries [2, 3]. However, adequate autologous vessels for bypass conduits are lacking in many patients. Compliance mismatch between veins and arteries contributes to myointimal hyperplasia, particularly at anastomotic sites, whilst thrombosis is a common problem. Thus, the need for compatible tissue-engineered small-diameter (