DMH groups two rats were sacrificed every 4 weeks until week 36. At week 40 all .... Wood CB, Habib NA, Apostolov K, Thompson A et al. (1985) Increase of ...
Col6i ee/al Disease
Int J Colorect Dis (1987) 2:12-14
9 Springer-Verlag 1987
Increased erythrocyte stearic acid desaturation in rats with chemically induced colorectal carcinomas N. A. Habib 1, M . J . Hershman 2, R. Salem 2, W. Barker 3, K. Apostolov 3 and C. B. W o o d 2 1 Department of Surgery, Bristol Royal Infirmary, Bristol, and Departments of 2 Surgery and 3 Virology, Royal Postgraduate Medical School, London, UK
Abstract. Our previous studies have demonstrated a desaturation of stearic acid related to oleic acid in the lipid layer of erythrocytes in patients with malignancies. This study investigated the stearic acid desaturation in red blood cell membranes of rats during the induction of colorectal tumours. Male Sprague-Dawley rats were injected weekly with dimethylhydrazine (DMH) and sacrificed at 4-week intervals. Blood was withdrawn via heart puncture, collected in EDTA bottle and erythrocytes separated by centrifugation. Total lipid extraction was carried out and analysed with gas liquid chromatography. In the control rats (injected with normal saline) the mean of the stearic to oleic acid ratio in erythrocyte membranes was 2.0_+ 0.3 (n = 28, range 1.51-2.62) compared to a mean of 0.94_+0.16 (n=0.5-1.23) in tumour bearing rats (p
