Increased Sensitivity in a Radioimmunoassay for ... - Clinical Chemistry

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Sep 11, 1989 - these fractions represent different effects of CSA in dif- ferent patients. References. 1. Matzke GR, Luke DR. Dialysis and renal transplantation.

mmolJL glycerol 3-phosphate was added to 900 L of dye produced from the cholesterol kit. In the absence of glycerol-3-phosphateoxidase, the glycerol 3-phosphate did not bleach this dye; evidently, the enzyme is indeed required as a catalyst. Further studies revealed that not only the presence but also the activity of glycerol-3-phosphate oxidase was important, because the quinonemonoimine dye was bleached only when the enzyme activity in the assay was 2.5 kUIL. Glycerol-3-phosphate oxidase from three different bacterial sources (Streptococcus thermophilus, Pediococcus spp. and Aerococcus uiridans) behaved similarly. 5. The effect of substituting other redox dyes for the Trinder-type chromogen was investigated. The colorless, oxidized form of iodonitrotetrazolium dye at a concentration of 160 moI/L was reduced by

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