peritoneal healing. 105 ..... This work was supported by the American Heart Association .... chemiluminescence produced by actived equine neutrophils. We.
INFLAMMATION
5. Inflammation 104 DIFFERENTIAL EXPRESSION OF MYELOPEROXIDASE (MPO) IN FIBROBLASTS ISOLATED FROM NORMAL PERITONEAL AND ADHESION TISSUES Ghassan Saed1, Michael Diamond1, and Husam Abu-Soud1 1 Wayne State University Injuries to the peritoneum, either because of infection, pathologic processes, and/or tissue trauma induced during surgical procedures result in development of adhesions in a vast majority of patients. MPO is a heme enzyme which uses hydrogen peroxide to produce HOCl, a highly reactive species with potent microbicidal and cytotoxic properties. While the generation of HOCl is of fundamental significancefor combating infection, sustained levels of this oxidant areassociated with side effects of inflammation, such as cell injuryand tissue damage. The objective of this study is to determine whether there is a difference in the expression levels of MPO in fibroblasts isolated from normal peritoneum and from adhesions, and whether this expression is modulated by hypoxia. We have obtained fibroblast primary cultures from normal peritoneum and adhesion tissues of the same patients. MPO synthesis by these fibroblasts was evaluated at mRNA utilizing the real-time RT/PCR and at the protein levels utilizing MPO ELISA kit. Fibroblasts were cultured under normal (20% O2) and hypoxic (2% O2) condition for 24 h. The studiesherein define normal fibroblasts to have higher MPO mRNA (32%) and protein (28%) levels than adhesion fibroblasts. Hypoxia decreased MPO mRNA by (35%) and protein levels by (40%; p
